Lag0037422 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0037422
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionHXXXD-type acyl-transferase family protein
Locationchr2: 6077956 .. 6081645 (-)
RNA-Seq ExpressionLag0037422
SyntenyLag0037422
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGGCGGCGGAAATAGCCTGATTTCCGGCCTGAAATTTTCGTCGGTGGTTCCGGCGAAGGCGACCGGCGAGAACAAGGTCCAGGAACTGACGGCGATCGATCTTGCGATGAAGCTTCATTACATTCGAGGCGTTTATTTCTTCAGGCCGAGCGAAGAGGTGAGGAATTTGACGATTTATGATCTGAAAAAACCTATGTTCCTGTTGCTGGAGCCGTACTACGTCGTTTCGGGAAGGATTCGACGGAGGATCGAAGATGGAGATCGGCCGTTCATCAAGTGTAATGATAGTGGAGTGAGAATCGTTGAAGCAGATTGTGAAAAAACGATCGACGAGTGGCTTTCGATCGAAGATGATGTGATTTCGCATCGCGATGGCGGTTTGGTTCACAGTCGAGCGATTGGTCCTGATCTTGGATTCTCCCCTCTCGTCTTCATACAGGTACGCTCATGTGCGTTGATTCGTTTTCATAACTTTTAACATAAATTCATTAAATTATTTTAAAAAGAAAAAAAAACATAAATTCATTAAAGATTTCTCTAAGTTTATTTATTTATTTATTTTAATTTCAAACTGATGATAATATATGCATGGCTTTAAAAAAAAAAAAAAAAAAACTGTGAAGATGAAAGTTTGTGAACGTTGAAGGTTTGAAGATTGAGTTTTCTAACCATAATACATAATACATAAAAGGTGAAGATTATAACCAAATGAAATTATCATTTGATTTTTAAAAATTGTGGTGGTTTTCTCTAAATCTTGATTTTCATTTTTGTTAAAGAGACATTTGAGTTTTTAGAAAACACTTTTTTTAAAACACTTTTTTTTTTTTTCAAAATTTGGTTTGGTTTTTTTAAAACATGAGGAAGGATGTAGATAAAAAAAAAAAAAAACAAATGAGTTTTGGATAAAAATAGTTTTTATAAACTTAATTTTCAAAAACAACAAACAAAGTGGTTATCAAACGGAGCCTTAGTCAATTGAATTTATCATATCTTTGGTTAGTTCAACTTTCATAACTTCCATATATTAAAAGGTTGGGTATTGGAATCTCTACTTTATATATTGTCAAACAAGAAAATTTGGGTTTTTTTTAGTTCAACAATATTTGAGTTGGGGAATTGACCTCTAATTCTCTTGACGAGATATATATGTCTCGAGTTATGTCTTGGCTATTCGACGGTCGACAAATTCTTAGTTTTTTTTTAGGAACAAATTCTCTTAATTATTTTATTGATTATTGTTTTGAAATTAATTATTGTAAAGTTTAAACGGGTGGATGGTTCATATCATTGATGTTATTTGTCATGTCCGATTCATTAGGATTTTAGGAATATATAATTTTAAGAACCAATGTTTAAAATTTGAATTAAATATCATTAACTAAAACTATAGTTTAAAAAACTTAACTATATTTCTTCTTTAAAAAATTTAAAAAAAAAAAACTAACAAACTATGGGATAAAAATTTAAGAGACCAAAACTATAGCAAATTTGAATAGTAAATTAATTAACGACAATCTATCATCTAAAATGGATGAGTGGAGCCTATCATATGGAGTTTTTTATTTTTAGAAAGCCTACCATATGGAGTTAATTTCATTAATGAAAGAAGATATATATATTTTATCTCTTAATTAAGAAGATAAGAAAGAAATTTGGAAATGAAATGATTAATTGTGAATATGACAACATGAACTATATGAACCGTGCAATAATATGTGATATTCGTTTTATATATATTAATGTCAAAATTGTTGAAGCTACAGCTTACAGGTTCCTCTTTAATGTCGGCGTTGGGGAAATTTCTCTTTCTCCACCTATCACAAAATCTCACGTGATATTTTTTTTTTTTTTTTTTTTTTGTTTAAGTATGAAAATATTGATTAAAAAACACATTTTTTTTTTGGTAAATATAATATCATTAAAATTGAATAGAGATACAACCACCCTCAATTAATTGAGGAAAAAATTAGTCTTTCTATTTTCATTTTTATAATCGTTTAGTCAACATGTAACAATTTTATTCTTGTACTCTAATATTGGTAACAATTTGATTTTTGTCGTCAATTAAACACAAAATCAAACCAAATATAATAGATATTTTTTTAAAAGATACTATACAAAGAATAAACATTTGAAGGTAGAGCTTTGTTTTTTTTTTTTTTTTTTTTTTTTTATAAATTTTGTTTAAAAGAATTTTCTGGCAAAGATTGAATCGTGAGATTTCAATTTCATATTTTTAAATTGATTCTAGGTTGATAACTATTTGGTTTTTTTTTTTTAATTTAAATTAAATTTGTTTTCATCTCATTTATTTTACATTTAATTAAAAACTAATGTATTATTAAATTATTATTTTAATAATTTATTTAGACACATAGACATGTCATGTCATTTATACACAATCTTTATGAAAAAAAAAAAACTTGAAATAACACAAGATTTATTATTATTATTTTTTATGTGCACGAATCAAATTTTGTCAATGACCTGATTGGGTTTCACGTATAGGTAGCCTCTAAACAATAGATATACTTGTTCAAATATGCCTCTAAAACAATGACTTTTTCCAATATTTAACAAGCTTTTAACATAAGGCCATCAAATGAGTATAACTCGACGATAATTGTTATGTACTTTAAATTTTAACCTCTCACCCCAAATATTGTTGAAAAAAAATGAATGTCCTAATTTAATCATAAGGTTTTTAACACACGAAGGGTAAATTAAAAAAAAAAATTAAAGATTAATACTCCAAAATCCATATAGTGAAAAATAATAATAAGTGAAAATGGATTTTGCCTGCACGCAGCTGACTCGGTTCAAGTGCGGCGGACTCGCCGTAGGCCTCAGTTGGGCTCACGTCCTCGGCGATATCTTCTCCGCCTCGACCTTCATCAACACATTGGGTCACATCATGAAAAACCGCCCGGTCGACCAACTCCGCCCACCGGCTGTGGCGGCGCCCCTCATCCGGCCGTCTCGCTCAACCGGACGGTCCACATTGCCAGTCAAGAGGCTCGATTCGACCGGAGACCTGTGGATCGGCTCGAGCGACTTCAAAATGGCGACGCGGTCGTTTCGGATCACGGAGGAGCAACTGGATCGAATCTTCAGCGTCGTCGGCCAGAATCGAGCGGCGAACTTCTCCAATTTCGAAGCTATTGCTGCGATTTTCTGGAAATCTCTGTCGAAAATACGGCTGGAGGACTCGGATTCCAAGACGATCTCGATCTATTCGACGAATTGCCCTAACAGAGAGGGCGAATCACCGAGGAACGGAATGGTGATGAGCGCCGTCGAGGCCGATTTTCCGGTCGCCGGAGCGGAGGAAGGTGCGCTGGCGGAGATGATTATGAAGAAGAAAATCGATGAGGGCGGAGAAATCGTGGAATTGGTGGAGAAAGAAAGGGAGGAGTCGGATTTCATAGCGTACGGAGCAAGATTGACGTTCGTTGATTTGGAAGAAGCGAATCTCTACGGCTTTGAATTGGAAGGACAGAAGCCAGTTCATGTGAATTATGAAACTGGAGGAGTTGGTGAAAACGGCGCCGTTTTGGTTCTACCAGGGCCGCCGCGTGGCGACGGGAGAGACGGCGGTGGCCGGACTGTGACGGTTATCTTGCCGGAGAAACAGCTGCCGGACTTGATCGATGAACTGCAGAAGCAATGGGCGATTGTTTGA

mRNA sequence

ATGGACGGCGGCGGAAATAGCCTGATTTCCGGCCTGAAATTTTCGTCGGTGGTTCCGGCGAAGGCGACCGGCGAGAACAAGGTCCAGGAACTGACGGCGATCGATCTTGCGATGAAGCTTCATTACATTCGAGGCGTTTATTTCTTCAGGCCGAGCGAAGAGGTGAGGAATTTGACGATTTATGATCTGAAAAAACCTATGTTCCTGTTGCTGGAGCCGTACTACGTCGTTTCGGGAAGGATTCGACGGAGGATCGAAGATGGAGATCGGCCGTTCATCAAGTGTAATGATAGTGGAGTGAGAATCGTTGAAGCAGATTGTGAAAAAACGATCGACGAGTGGCTTTCGATCGAAGATGATGTGATTTCGCATCGCGATGGCGGTTTGGTTCACAGTCGAGCGATTGGTCCTGATCTTGGATTCTCCCCTCTCGTCTTCATACAGCTGACTCGGTTCAAGTGCGGCGGACTCGCCGTAGGCCTCAGTTGGGCTCACGTCCTCGGCGATATCTTCTCCGCCTCGACCTTCATCAACACATTGGGTCACATCATGAAAAACCGCCCGGTCGACCAACTCCGCCCACCGGCTGTGGCGGCGCCCCTCATCCGGCCGTCTCGCTCAACCGGACGGTCCACATTGCCAGTCAAGAGGCTCGATTCGACCGGAGACCTGTGGATCGGCTCGAGCGACTTCAAAATGGCGACGCGGTCGTTTCGGATCACGGAGGAGCAACTGGATCGAATCTTCAGCGTCGTCGGCCAGAATCGAGCGGCGAACTTCTCCAATTTCGAAGCTATTGCTGCGATTTTCTGGAAATCTCTGTCGAAAATACGGCTGGAGGACTCGGATTCCAAGACGATCTCGATCTATTCGACGAATTGCCCTAACAGAGAGGGCGAATCACCGAGGAACGGAATGGTGATGAGCGCCGTCGAGGCCGATTTTCCGGTCGCCGGAGCGGAGGAAGGTGCGCTGGCGGAGATGATTATGAAGAAGAAAATCGATGAGGGCGGAGAAATCGTGGAATTGGTGGAGAAAGAAAGGGAGGAGTCGGATTTCATAGCGTACGGAGCAAGATTGACGTTCGTTGATTTGGAAGAAGCGAATCTCTACGGCTTTGAATTGGAAGGACAGAAGCCAGTTCATGTGAATTATGAAACTGGAGGAGTTGGTGAAAACGGCGCCGTTTTGGTTCTACCAGGGCCGCCGCGTGGCGACGGGAGAGACGGCGGTGGCCGGACTGTGACGGTTATCTTGCCGGAGAAACAGCTGCCGGACTTGATCGATGAACTGCAGAAGCAATGGGCGATTGTTTGA

Coding sequence (CDS)

ATGGACGGCGGCGGAAATAGCCTGATTTCCGGCCTGAAATTTTCGTCGGTGGTTCCGGCGAAGGCGACCGGCGAGAACAAGGTCCAGGAACTGACGGCGATCGATCTTGCGATGAAGCTTCATTACATTCGAGGCGTTTATTTCTTCAGGCCGAGCGAAGAGGTGAGGAATTTGACGATTTATGATCTGAAAAAACCTATGTTCCTGTTGCTGGAGCCGTACTACGTCGTTTCGGGAAGGATTCGACGGAGGATCGAAGATGGAGATCGGCCGTTCATCAAGTGTAATGATAGTGGAGTGAGAATCGTTGAAGCAGATTGTGAAAAAACGATCGACGAGTGGCTTTCGATCGAAGATGATGTGATTTCGCATCGCGATGGCGGTTTGGTTCACAGTCGAGCGATTGGTCCTGATCTTGGATTCTCCCCTCTCGTCTTCATACAGCTGACTCGGTTCAAGTGCGGCGGACTCGCCGTAGGCCTCAGTTGGGCTCACGTCCTCGGCGATATCTTCTCCGCCTCGACCTTCATCAACACATTGGGTCACATCATGAAAAACCGCCCGGTCGACCAACTCCGCCCACCGGCTGTGGCGGCGCCCCTCATCCGGCCGTCTCGCTCAACCGGACGGTCCACATTGCCAGTCAAGAGGCTCGATTCGACCGGAGACCTGTGGATCGGCTCGAGCGACTTCAAAATGGCGACGCGGTCGTTTCGGATCACGGAGGAGCAACTGGATCGAATCTTCAGCGTCGTCGGCCAGAATCGAGCGGCGAACTTCTCCAATTTCGAAGCTATTGCTGCGATTTTCTGGAAATCTCTGTCGAAAATACGGCTGGAGGACTCGGATTCCAAGACGATCTCGATCTATTCGACGAATTGCCCTAACAGAGAGGGCGAATCACCGAGGAACGGAATGGTGATGAGCGCCGTCGAGGCCGATTTTCCGGTCGCCGGAGCGGAGGAAGGTGCGCTGGCGGAGATGATTATGAAGAAGAAAATCGATGAGGGCGGAGAAATCGTGGAATTGGTGGAGAAAGAAAGGGAGGAGTCGGATTTCATAGCGTACGGAGCAAGATTGACGTTCGTTGATTTGGAAGAAGCGAATCTCTACGGCTTTGAATTGGAAGGACAGAAGCCAGTTCATGTGAATTATGAAACTGGAGGAGTTGGTGAAAACGGCGCCGTTTTGGTTCTACCAGGGCCGCCGCGTGGCGACGGGAGAGACGGCGGTGGCCGGACTGTGACGGTTATCTTGCCGGAGAAACAGCTGCCGGACTTGATCGATGAACTGCAGAAGCAATGGGCGATTGTTTGA

Protein sequence

MDGGGNSLISGLKFSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTIYDLKKPMFLLLEPYYVVSGRIRRRIEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDDVISHRDGGLVHSRAIGPDLGFSPLVFIQLTRFKCGGLAVGLSWAHVLGDIFSASTFINTLGHIMKNRPVDQLRPPAVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMATRSFRITEEQLDRIFSVVGQNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNREGESPRNGMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGARLTFVDLEEANLYGFELEGQKPVHVNYETGGVGENGAVLVLPGPPRGDGRDGGGRTVTVILPEKQLPDLIDELQKQWAIV
Homology
BLAST of Lag0037422 vs. NCBI nr
Match: XP_022980996.1 (protein ECERIFERUM 2 [Cucurbita maxima])

HSP 1 Score: 753.4 bits (1944), Expect = 1.1e-213
Identity = 382/439 (87.02%), Postives = 403/439 (91.80%), Query Frame = 0

Query: 1   MDGGGNSLISGLKFSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTI 60
           MDG GN LIS +KFSSVVPAKATGENKV EL+AIDLAMKLHYIRGVYFFRPSEEVRNLTI
Sbjct: 1   MDGSGNDLISEVKFSSVVPAKATGENKVHELSAIDLAMKLHYIRGVYFFRPSEEVRNLTI 60

Query: 61  YDLKKPMFLLLEPYYVVSGRIRRRIEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDD 120
           YDLKKPMF LLE YYVVSGRIRRRIED DRPFIKCNDSGVRIVE +CEKTIDEWLSI+DD
Sbjct: 61  YDLKKPMFPLLEIYYVVSGRIRRRIEDRDRPFIKCNDSGVRIVEVECEKTIDEWLSIDDD 120

Query: 121 VISHRDGGLVHSRAIGPDLGFSPLVFIQLTRFKCGGLAVGLSWAHVLGDIFSASTFINTL 180
            ISHRDG LV+SRAIGPDLGFSPLVFIQLTRF+CGGL+VGLSWAHVLGDIFSASTFIN  
Sbjct: 121 KISHRDGDLVYSRAIGPDLGFSPLVFIQLTRFRCGGLSVGLSWAHVLGDIFSASTFINAW 180

Query: 181 GHIMKNRPVDQLRP-PAVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMATRSFR 240
           GH+MKNRPV++LRP PA AA LIRPS STGRST P+KRLDSTGDLWI S+D KMA RSFR
Sbjct: 181 GHVMKNRPVERLRPLPATAADLIRPSGSTGRSTSPIKRLDSTGDLWIASNDCKMAKRSFR 240

Query: 241 ITEEQLDRIFSVVGQNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNREG 300
           I EEQL RI SVV +NR+AN+S+FEAIAAIFWK LSKIRLEDSDSKTISIYST CP+REG
Sbjct: 241 IMEEQLVRILSVVDRNRSANYSSFEAIAAIFWKCLSKIRLEDSDSKTISIYSTKCPDREG 300

Query: 301 ESPRNGMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGAR 360
           + PRNGMVMSAVEADFPVAGAEEGALAE+I+KKKIDE  EI ELVEKEREESDFIAYGAR
Sbjct: 301 QIPRNGMVMSAVEADFPVAGAEEGALAELIVKKKIDEDEEIKELVEKEREESDFIAYGAR 360

Query: 361 LTFVDLEEANLYGFELEGQKPVHVNYETGGVGENGAVLVLPGPPRGDGRDGGGRTVTVIL 420
           LTFVDLEEANLYG ELEGQ+PVHVNYE GGVGENG VLVLPGPPR DG  GGGRTVTVIL
Sbjct: 361 LTFVDLEEANLYGLELEGQRPVHVNYEIGGVGENGVVLVLPGPPRCDGTPGGGRTVTVIL 420

Query: 421 PEKQLPDLIDELQKQWAIV 439
           PEKQLPDLIDELQKQWAIV
Sbjct: 421 PEKQLPDLIDELQKQWAIV 439

BLAST of Lag0037422 vs. NCBI nr
Match: KAG6608131.1 (Protein ECERIFERUM 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 751.5 bits (1939), Expect = 4.0e-213
Identity = 378/439 (86.10%), Postives = 405/439 (92.26%), Query Frame = 0

Query: 1   MDGGGNSLISGLKFSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTI 60
           MDG GN LIS +KFSSVVPAKATGENKV EL+AIDLAMKLHYIRGVYFFRPSEE+RNLTI
Sbjct: 1   MDGSGNELISEVKFSSVVPAKATGENKVHELSAIDLAMKLHYIRGVYFFRPSEEMRNLTI 60

Query: 61  YDLKKPMFLLLEPYYVVSGRIRRRIEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDD 120
           YDLKKPMF LLE YYVVSGRIRRRIED DRPFIKCNDSGVRIVE +CEKTIDEWLSI+DD
Sbjct: 61  YDLKKPMFPLLEIYYVVSGRIRRRIEDRDRPFIKCNDSGVRIVEVECEKTIDEWLSIDDD 120

Query: 121 VISHRDGGLVHSRAIGPDLGFSPLVFIQLTRFKCGGLAVGLSWAHVLGDIFSASTFINTL 180
            ISHRDG LV+SRAIGPDLGFSPLVFIQLTRF+CGGL+VGLSWAHVLGDIFSASTFIN  
Sbjct: 121 KISHRDGDLVYSRAIGPDLGFSPLVFIQLTRFRCGGLSVGLSWAHVLGDIFSASTFINAW 180

Query: 181 GHIMKNRPVDQLRP-PAVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMATRSFR 240
           GH+MKNRPV++LRP PA AA LIRPS STGRST P+KRLDSTGDLWI S+D KMA RSFR
Sbjct: 181 GHVMKNRPVERLRPLPATAADLIRPSGSTGRSTSPIKRLDSTGDLWIASNDCKMAKRSFR 240

Query: 241 ITEEQLDRIFSVVGQNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNREG 300
           ITEE+L RI SVV +NR+AN+S+FEAIAAIFWK LSKIRLEDSDSKTISIYST CP+REG
Sbjct: 241 ITEEKLVRILSVVDRNRSANYSSFEAIAAIFWKCLSKIRLEDSDSKTISIYSTKCPDREG 300

Query: 301 ESPRNGMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGAR 360
           + PRNGMVMSA+EADFPVAGAEEGALAE+I+KKKIDEG EI ELVEKERE+SDFIAYGAR
Sbjct: 301 QIPRNGMVMSAIEADFPVAGAEEGALAELIVKKKIDEGEEIKELVEKEREDSDFIAYGAR 360

Query: 361 LTFVDLEEANLYGFELEGQKPVHVNYETGGVGENGAVLVLPGPPRGDGRDGGGRTVTVIL 420
           LTFVDLEEANLYG E+EGQ+PVHVNYE GGVGENG VLVLPGPPR DGR GG RTVTVIL
Sbjct: 361 LTFVDLEEANLYGLEMEGQRPVHVNYEIGGVGENGVVLVLPGPPRCDGRPGGDRTVTVIL 420

Query: 421 PEKQLPDLIDELQKQWAIV 439
           PEKQLP+LIDELQKQWAIV
Sbjct: 421 PEKQLPELIDELQKQWAIV 439

BLAST of Lag0037422 vs. NCBI nr
Match: XP_022940107.1 (protein ECERIFERUM 2 [Cucurbita moschata] >KAG7031769.1 Protein ECERIFERUM 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 750.4 bits (1936), Expect = 9.0e-213
Identity = 379/439 (86.33%), Postives = 404/439 (92.03%), Query Frame = 0

Query: 1   MDGGGNSLISGLKFSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTI 60
           MDG GN LIS +KFSSVVPAKATGENKV EL+AIDLAMKLHYIRGVYFFRPSEEVRNLTI
Sbjct: 1   MDGSGNELISEVKFSSVVPAKATGENKVHELSAIDLAMKLHYIRGVYFFRPSEEVRNLTI 60

Query: 61  YDLKKPMFLLLEPYYVVSGRIRRRIEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDD 120
           YDLKKPMF LLE YYVVSGRIRRRIED DRPFIKCNDSGVRIVE +CEKTIDEWLSI+DD
Sbjct: 61  YDLKKPMFPLLEIYYVVSGRIRRRIEDRDRPFIKCNDSGVRIVEVECEKTIDEWLSIDDD 120

Query: 121 VISHRDGGLVHSRAIGPDLGFSPLVFIQLTRFKCGGLAVGLSWAHVLGDIFSASTFINTL 180
            ISHRDG LV+SRAIGPDLGFSPLVFIQLTRF+CGGL+VGLSWAHVLGDIFSASTFIN  
Sbjct: 121 KISHRDGDLVYSRAIGPDLGFSPLVFIQLTRFRCGGLSVGLSWAHVLGDIFSASTFINAW 180

Query: 181 GHIMKNRPVDQLRP-PAVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMATRSFR 240
           GH+MKNRPV++LRP PA AA LIRPS STGRST P+KRLDSTGDLWI S+D KMA RSFR
Sbjct: 181 GHVMKNRPVERLRPLPATAADLIRPSGSTGRSTSPIKRLDSTGDLWIASNDCKMAKRSFR 240

Query: 241 ITEEQLDRIFSVVGQNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNREG 300
           ITEE+L RI SVV +NR+AN+S+FEAIAAIFWK LSKIRLEDSDSKTISIYST CP+REG
Sbjct: 241 ITEEKLVRILSVVDRNRSANYSSFEAIAAIFWKCLSKIRLEDSDSKTISIYSTKCPDREG 300

Query: 301 ESPRNGMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGAR 360
           + P NGMVMSA+EADFPVAGAEEGALAE+I+KKKIDEG EI ELVEKERE+SDFIAYGAR
Sbjct: 301 QIPINGMVMSAIEADFPVAGAEEGALAELIVKKKIDEGEEIKELVEKEREDSDFIAYGAR 360

Query: 361 LTFVDLEEANLYGFELEGQKPVHVNYETGGVGENGAVLVLPGPPRGDGRDGGGRTVTVIL 420
           LTFVDLEEANLYG ELEGQ+PVHVNYE GGVGENG VLVLPGPPR DGR GG RTVTVIL
Sbjct: 361 LTFVDLEEANLYGLELEGQRPVHVNYEIGGVGENGVVLVLPGPPRCDGRPGGDRTVTVIL 420

Query: 421 PEKQLPDLIDELQKQWAIV 439
           PEKQLP+LIDELQKQWAIV
Sbjct: 421 PEKQLPELIDELQKQWAIV 439

BLAST of Lag0037422 vs. NCBI nr
Match: XP_023523388.1 (protein ECERIFERUM 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 745.3 bits (1923), Expect = 2.9e-211
Identity = 380/439 (86.56%), Postives = 400/439 (91.12%), Query Frame = 0

Query: 1   MDGGGNSLISGLKFSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTI 60
           MDG GN LIS +K SSVVPAKATGENKV EL+AIDLAMKLHYIRGVYFFRPSEEVRNLTI
Sbjct: 1   MDGSGNDLISEVKLSSVVPAKATGENKVHELSAIDLAMKLHYIRGVYFFRPSEEVRNLTI 60

Query: 61  YDLKKPMFLLLEPYYVVSGRIRRRIEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDD 120
           YDLKKPMF LLE YYVVSGRIRRRIED DRPFIKCNDSGVRIVE +CEKTIDEWLSI+DD
Sbjct: 61  YDLKKPMFPLLEIYYVVSGRIRRRIEDRDRPFIKCNDSGVRIVEVECEKTIDEWLSIDDD 120

Query: 121 VISHRDGGLVHSRAIGPDLGFSPLVFIQLTRFKCGGLAVGLSWAHVLGDIFSASTFINTL 180
            ISHR G LV+SRAIGPDLGFSPLVFIQLTRF+CGGL+VGLSWAHVLGDIFSAS FIN  
Sbjct: 121 KISHRHGDLVYSRAIGPDLGFSPLVFIQLTRFRCGGLSVGLSWAHVLGDIFSASNFINAW 180

Query: 181 GHIMKNRPVDQLRP-PAVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMATRSFR 240
           GH+MKNRPV++LRP PA AA LIRPS STGRST PVKRLDSTGDLWI S+D KMA RSFR
Sbjct: 181 GHVMKNRPVERLRPLPATAADLIRPSGSTGRSTSPVKRLDSTGDLWIASNDCKMANRSFR 240

Query: 241 ITEEQLDRIFSVVGQNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNREG 300
           ITEEQL RI SVV +NR+AN+S+FEAIAAIFWK LSKIRLEDSDSKTISIYST  P+REG
Sbjct: 241 ITEEQLVRILSVVDRNRSANYSSFEAIAAIFWKCLSKIRLEDSDSKTISIYSTKFPDREG 300

Query: 301 ESPRNGMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGAR 360
           + PRNGMVMSAVEADFPVAGAEEGALAE+I+KKKIDEG EI ELVEKEREESDFIAYGAR
Sbjct: 301 QIPRNGMVMSAVEADFPVAGAEEGALAELIVKKKIDEGEEIKELVEKEREESDFIAYGAR 360

Query: 361 LTFVDLEEANLYGFELEGQKPVHVNYETGGVGENGAVLVLPGPPRGDGRDGGGRTVTVIL 420
           LTFVDLEEANLYG ELEGQ+PVHVNYE GGVGENG VLVLPGPPR DG  GG RTVTVIL
Sbjct: 361 LTFVDLEEANLYGLELEGQRPVHVNYEIGGVGENGVVLVLPGPPRCDGTPGGDRTVTVIL 420

Query: 421 PEKQLPDLIDELQKQWAIV 439
           PEKQLPDLIDELQKQWAIV
Sbjct: 421 PEKQLPDLIDELQKQWAIV 439

BLAST of Lag0037422 vs. NCBI nr
Match: XP_038898516.1 (protein ECERIFERUM 2 [Benincasa hispida])

HSP 1 Score: 722.2 bits (1863), Expect = 2.6e-204
Identity = 370/437 (84.67%), Postives = 386/437 (88.33%), Query Frame = 0

Query: 2   DGGGNSLISGLKFSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTIY 61
           DG  N+LIS LKFSSVVPAKATG+NKV+ELTAIDLAMKLHYIRGVYFFR SEEVRNLTIY
Sbjct: 3   DGSKNTLISELKFSSVVPAKATGDNKVKELTAIDLAMKLHYIRGVYFFRASEEVRNLTIY 62

Query: 62  DLKKPMFLLLEPYYVVSGRIRRRIEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDDV 121
           DLKKP+F LLE YYVVSGRIRRRIED DRPFIKCNDSGVRIVEADCEKTI+EWLSI DD 
Sbjct: 63  DLKKPLFQLLELYYVVSGRIRRRIEDEDRPFIKCNDSGVRIVEADCEKTIEEWLSIGDDK 122

Query: 122 ISHRDGGLVHSRAIGPDLGFSPLVFIQLTRFKCGGLAVGLSWAHVLGDIFSASTFINTLG 181
           ISHRD  LVHS+AIGPDLGFSPL FIQLTRFKCGGL+VGLSW HVLGDIFSASTFIN  G
Sbjct: 123 ISHRDDCLVHSQAIGPDLGFSPLAFIQLTRFKCGGLSVGLSWTHVLGDIFSASTFINVWG 182

Query: 182 HIMKNRPVDQLRPPAVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMATRSFRIT 241
           +IM NRP D L P    A L RPSRST  ST PVKRLD TGDLWIGSSD KMATRSFRIT
Sbjct: 183 YIMNNRPADHLLP--APAALTRPSRSTRLSTPPVKRLDPTGDLWIGSSDCKMATRSFRIT 242

Query: 242 EEQLDRIFSVVGQNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNREGES 301
            EQLDRI  V G+NRA NFS FEAIAAIFWK LSKIRLEDS+S+TISIYST  PNREGE 
Sbjct: 243 AEQLDRILGVFGRNRAVNFSTFEAIAAIFWKFLSKIRLEDSNSRTISIYSTKFPNREGEI 302

Query: 302 PRNGMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGARLT 361
           PRNGM MS VEA+FPVAGA EG LAEMI+KKKIDEGGEI  LVEKER+ESDFIAYGARLT
Sbjct: 303 PRNGMEMSGVEAEFPVAGAAEGELAEMIVKKKIDEGGEIGGLVEKERDESDFIAYGARLT 362

Query: 362 FVDLEEANLYGFELEGQKPVHVNYETGGVGENGAVLVLPGPPRGDGRDGGGRTVTVILPE 421
           FVDLEEAN+YGFELEGQKPVHVNYE GGVGENG VLVLPGPP GDGRDGGG TVTVILPE
Sbjct: 363 FVDLEEANIYGFELEGQKPVHVNYEIGGVGENGVVLVLPGPPCGDGRDGGGLTVTVILPE 422

Query: 422 KQLPDLIDELQKQWAIV 439
           K+LPDLIDELQKQW IV
Sbjct: 423 KELPDLIDELQKQWGIV 437

BLAST of Lag0037422 vs. ExPASy Swiss-Prot
Match: Q39048 (Protein ECERIFERUM 2 OS=Arabidopsis thaliana OX=3702 GN=CER2 PE=1 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 5.9e-82
Identity = 175/435 (40.23%), Postives = 262/435 (60.23%), Query Frame = 0

Query: 7   SLISGLKFSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTIYDLKKP 66
           S ++ ++ SSVVPA   GENK ++LT +DLAMKLHY+R VYFF+ +   R+ T+ D+K  
Sbjct: 4   SPVTSVRLSSVVPASVVGENKPRQLTPMDLAMKLHYVRAVYFFKGA---RDFTVADVKNT 63

Query: 67  MFL---LLEPYYVVSGRIRRRIEDGDR-----PFIKCNDSGVRIVEADCEK-TIDEWLSI 126
           MF    LL+ Y+ VSGRIR    D D      P+I+CNDSG+R+VEA+ E+ T+++WL +
Sbjct: 64  MFTLQSLLQSYHHVSGRIRMSDNDNDTSAAAIPYIRCNDSGIRVVEANVEEFTVEKWLEL 123

Query: 127 EDDVISHRDGGLVHSRAIGPDLGFSPLVFIQLTRFKCGGLAVGLSWAHVLGDIFSASTFI 186
           +D  I HR   LV+   +GPDL FSPLVF+Q+T+FKCGGL +GLSWAH+LGD+FSASTF+
Sbjct: 124 DDRSIDHR--FLVYDHVLGPDLTFSPLVFLQITQFKCGGLCIGLSWAHILGDVFSASTFM 183

Query: 187 NTLGHIMKNRPVDQLRPPAVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMATRS 246
            TLG ++        +P     P +          + ++++DS G+ W+ ++  KM    
Sbjct: 184 KTLGQLVSGHA--PTKPVYPKTPELTSHARNDGEAISIEKIDSVGEYWLLTNKCKMGRHI 243

Query: 247 FRITEEQLDRIFSVVGQNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNR 306
           F  +   +D + +     R   FS  + + A+ WKSL  IR E +++  I+I    C  +
Sbjct: 244 FNFSLNHIDSLMAKY-TTRDQPFSEVDILYALIWKSLLNIRGE-TNTNVITI----CDRK 303

Query: 307 EGESPRN-GMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAY 366
           +  +  N  +V+S VE +  + G  E  LA +I  +K +E G I  ++E+++  SDF  Y
Sbjct: 304 KSSTCWNEDLVISVVEKNDEMVGISE--LAALIAGEKREENGAIKRMIEQDKGSSDFFTY 363

Query: 367 GARLTFVDLEEANLYGFELEGQKPVHVNYETGGVGENGAVLVLPGPPRGDGRDGGGRTVT 426
           GA LTFV+L+E ++Y  E+ G KP  VNY   GVG+ G VLV P       +    R V+
Sbjct: 364 GANLTFVNLDEIDMYELEINGGKPDFVNYTIHGVGDKGVVLVFP-------KQNFARIVS 416

Query: 427 VILPEKQLPDLIDEL 432
           V++PE+ L  L +E+
Sbjct: 424 VVMPEEDLAKLKEEV 416

BLAST of Lag0037422 vs. ExPASy Swiss-Prot
Match: Q9LIS1 (Protein ECERIFERUM 26-like OS=Arabidopsis thaliana OX=3702 GN=CER26L PE=2 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 7.5e-69
Identity = 157/429 (36.60%), Postives = 237/429 (55.24%), Query Frame = 0

Query: 5   GNSLISGLKFSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTIYDLK 64
           G+  + G + S+V  +  +      E T +DLAMKLHY++ VY +  +   R+LT+ D+K
Sbjct: 8   GSGPVHGFRLSTVSASLPSETGTTHEPTGLDLAMKLHYLKAVYIY-SAGTARDLTVMDVK 67

Query: 65  KPMFLLLEPYYVVSGRIRRRIEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDDVISH 124
            P+F +      + GR RR   +  RP++KCND G R VE+ C+ T++EWL + D  +  
Sbjct: 68  APLFSVFYQIPCIIGRFRR--HESGRPYLKCNDCGTRFVESHCDLTVEEWLRVPDRSV-- 127

Query: 125 RDGGLVHSRAIGPDLGFSPLVFIQLTRFKCGGLAVGLSWAHVLGDIFSASTFINTLGHIM 184
            D  LV+ + +GPDL FSPL++IQ+TRF CGGLA+GLSWAH++GD FS S F N      
Sbjct: 128 -DESLVYHQPVGPDLAFSPLLYIQMTRFSCGGLALGLSWAHIMGDPFSLSHFFNLWAQAF 187

Query: 185 KNRPVDQLRPPAVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMATRSFRITEEQ 244
               +   +          P+ ST +    VK++D  GDLW+  ++ KM T SF +T   
Sbjct: 188 AGGKIYCPKTSVTERDFQNPT-STFKKPDSVKQVDLVGDLWVAPNNSKMTTFSFNLTVND 247

Query: 245 LDRIFSVVGQNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNREGESPRN 304
           L   F V G         FE +  I WK ++ +R E S   TI++  ++    +  + RN
Sbjct: 248 LKTHFPVNGD------GEFEILTGIIWKCVATVRGE-SAPVTITVIRSDPKKLKPRAVRN 307

Query: 305 GMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGARLTFVD 364
           G ++S++  DF VA A    + + I + K DE   I E+V+   + SDFI YGA LTFVD
Sbjct: 308 GQMISSIHVDFSVAEASLEEIVKSIGEAK-DERVVIDEIVD---DVSDFIVYGANLTFVD 367

Query: 365 LEEANLYGFELEGQKPVHVNYETGGVGENGAVLVLPGPPRGDGRDGGGRTVTVILPEKQL 424
           + E + Y  ++ G+ P  V     G+G++GAV+VLPG    +      R VTV LP  ++
Sbjct: 368 MSEVDFYEAKVMGKSPESVYCNVQGIGDDGAVVVLPGVVEEE------RVVTVTLPVDEI 412

Query: 425 PDLIDELQK 434
             +  E++K
Sbjct: 428 EKVKWEMKK 412

BLAST of Lag0037422 vs. ExPASy Swiss-Prot
Match: Q9SVM9 (Protein ECERIFERUM 26 OS=Arabidopsis thaliana OX=3702 GN=CER26 PE=2 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 1.0e-65
Identity = 152/432 (35.19%), Postives = 233/432 (53.94%), Query Frame = 0

Query: 3   GGGNSLISGLKFSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTIYD 62
           G G   +  ++ S+V   + T      E T +DLAMKLHY++  Y +  +E  R+LT+  
Sbjct: 8   GQGQGPVHSIRLSTVGATRPTETGTTHEPTGLDLAMKLHYLKAAYIY-SAETARDLTVRH 67

Query: 63  LKKPMFLLLEPYYVVSGRIRRRIEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDDVI 122
           LK+ MF+L +     +GR  RR  D  RP+IKCND G R VE  C  T++EWLS  D  +
Sbjct: 68  LKEAMFMLFDQIAWTTGRFSRR--DSGRPYIKCNDCGTRFVEGQCNLTVEEWLSKPDRSV 127

Query: 123 SHRDGGLVHSRAIGPDLGFSPLVFIQLTRFKCGGLAVGLSWAHVLGDIFSASTFINTLGH 182
              D  LV+   IGP+L FSPL+++Q+TRFKCGGL +GLSWA+++GD FS     N    
Sbjct: 128 ---DEFLVYHHPIGPELTFSPLIYVQMTRFKCGGLGLGLSWANIIGDAFSLFYAFNLWAK 187

Query: 183 IMKNRPVDQLRPPAVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMATRSFRIT- 242
            +    +     P++     +    T +  + +KR++  GDLW+  +D K+A   F ++ 
Sbjct: 188 AITGEKIYAPTTPSIGERRFQSPNPTVKDPVSIKRVEPVGDLWVTPNDKKLANYCFNLSV 247

Query: 243 EEQLDRIFSVVGQNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNREGES 302
            +Q+   F   G +   +   FE +A I WK ++K+R+E     T++I   +  + +  +
Sbjct: 248 ADQISPHFPAKGDD---SIPVFEILAGIIWKCIAKVRVEPK-PVTVTIIKKDPNDLKLNA 307

Query: 303 PRNGMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGARLT 362
            RN  V+S+V  DFPVA A    L + + + K DE   I E+ E      DF+ YGA+LT
Sbjct: 308 IRNSQVISSVSVDFPVAEATVEELVKAMGEAK-DERCGIEEIGESCDGNLDFVVYGAKLT 367

Query: 363 FVDLEEANLYGFELEGQKPVHVNYETGGVGENGAVLVLPGPPRGDGRDGGGRTVTVILPE 422
           F+DL   +LY  ++ G+ P  V     G+GE G V+V       +      R VTV LPE
Sbjct: 368 FLDLTGEDLYEAKVMGKSPESVYCNVEGIGEEGLVVVYAAAKSEE------RVVTVTLPE 422

Query: 423 KQLPDLIDELQK 434
           +++  +  E +K
Sbjct: 428 EEMERVKLEFKK 422

BLAST of Lag0037422 vs. ExPASy Swiss-Prot
Match: A0A2H5AIZ1 (Hydroxycinnamoyltransferase OS=Narcissus pseudonarcissus OX=39639 GN=HCT PE=2 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 9.2e-19
Identity = 91/332 (27.41%), Postives = 150/332 (45.18%), Query Frame = 0

Query: 8   LISGLKFSSVV-PAKATGENKVQELTAIDLAMKLHYIRGVYFF-RPSEEVRNLTIYDLKK 67
           +I  +K +++V P++ T   ++   + +DL +   +   VYF+ RP           LK+
Sbjct: 1   MIINIKETTMVRPSQPTPSQRLWN-SNLDLVVPRFHTPSVYFYRRPGNASDFFDARVLKE 60

Query: 68  PMFLLLEPYYVVSGRIRRRIEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDDVISHR 127
            +   L P+Y ++GR+ R  EDG R  I CN  GVR V A+ +  IDE+      +   +
Sbjct: 61  ALGRALVPFYPMAGRLARD-EDG-RVEIDCNGEGVRFVVAETDSAIDEFGDFAPTMELKK 120

Query: 128 DGGLVHSRAIGPDLGFSPLVFIQLTRFKCGGLAVGLSWAHVLGDIFSASTFINTLGHIMK 187
              L+     G D+   PL+ +Q+T FKCGG ++G+   H + D  S   FIN+   I  
Sbjct: 121 ---LIPKVEYGDDISAFPLLVLQITHFKCGGTSLGVGMQHHVADGASGLHFINSWSDIA- 180

Query: 188 NRPVDQLRPPAVAAPLIR---------------PSRSTGRSTLPVKRLDSTGDLWIGSSD 247
            R +D   PP +   L+R               P+ S   S  P++      D  + S  
Sbjct: 181 -RGLDIAVPPFIDRSLLRARDPPSPSFPHIEYQPAPSMNTSPAPIQ------DPTVKSDP 240

Query: 248 FKMATRSFRITEEQLDRIFSVVGQNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIY 307
              A   F++T++QLD + S V    +A +S++  +A   W+  S  R    D +T    
Sbjct: 241 TATAVSIFKLTKQQLDLLKSRV----SAKYSSYALVAGHVWRCTSIARGLPDDQRTKLYC 300

Query: 308 STNCPNREGESPRNGMVMSAVEADFPVAGAEE 323
           +T+   R      +G   + +    PVA A E
Sbjct: 301 ATDGRARLQPPLPSGYFGNVIFTATPVADAGE 314

BLAST of Lag0037422 vs. ExPASy Swiss-Prot
Match: Q9FI78 (Shikimate O-hydroxycinnamoyltransferase OS=Arabidopsis thaliana OX=3702 GN=HST PE=1 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 7.3e-16
Identity = 73/278 (26.26%), Postives = 128/278 (46.04%), Query Frame = 0

Query: 34  IDLAMKLHYIRGVYFFRPSEEVRNLTIYDLKKPMFLLLEPYYVVSGRIRRRIEDGDRPFI 93
           +DL +   +   VYF+RP+          +K+ +   L P+Y ++GR++R  +D  R  I
Sbjct: 27  VDLVIPRFHTPSVYFYRPTGASNFFDPQVMKEALSKALVPFYPMAGRLKR--DDDGRIEI 86

Query: 94  KCNDSGVRIVEADCEKTIDEW------LSIEDDV--ISHRDGGLVHSRAIGPDLGFSPLV 153
            CN +GV  V AD    ID++      L++   +  + H  G  +HS          PL+
Sbjct: 87  DCNGAGVLFVVADTPSVIDDFGDFAPTLNLRQLIPEVDHSAG--IHS---------FPLL 146

Query: 154 FIQLTRFKCGGLAVGLSWAHVLGDIFSASTFINTLGHIMKNRPVDQLRPPAVAAPLIRPS 213
            +Q+T FKCGG ++G+   H   D FS   FINT   +   R +D   PP +   L+R +
Sbjct: 147 VLQVTFFKCGGASLGVGMQHHAADGFSGLHFINTWSDMA--RGLDLTIPPFIDRTLLR-A 206

Query: 214 RSTGRSTLPVKRLDSTGDLWIGSSDFKMATRS-----FRITEEQLDRIFSVVGQN-RAAN 273
           R   +             + I     K    +     F++T +QL  + +   ++    +
Sbjct: 207 RDPPQPAFHHVEYQPAPSMKIPLDPSKSGPENTTVSIFKLTRDQLVALKAKSKEDGNTVS 266

Query: 274 FSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNR 298
           +S++E +A   W+S+ K R   +D +T    +T+  +R
Sbjct: 267 YSSYEMLAGHVWRSVGKARGLPNDQETKLYIATDGRSR 288

BLAST of Lag0037422 vs. ExPASy TrEMBL
Match: A0A6J1IV89 (protein ECERIFERUM 2 OS=Cucurbita maxima OX=3661 GN=LOC111480277 PE=3 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 5.1e-214
Identity = 382/439 (87.02%), Postives = 403/439 (91.80%), Query Frame = 0

Query: 1   MDGGGNSLISGLKFSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTI 60
           MDG GN LIS +KFSSVVPAKATGENKV EL+AIDLAMKLHYIRGVYFFRPSEEVRNLTI
Sbjct: 1   MDGSGNDLISEVKFSSVVPAKATGENKVHELSAIDLAMKLHYIRGVYFFRPSEEVRNLTI 60

Query: 61  YDLKKPMFLLLEPYYVVSGRIRRRIEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDD 120
           YDLKKPMF LLE YYVVSGRIRRRIED DRPFIKCNDSGVRIVE +CEKTIDEWLSI+DD
Sbjct: 61  YDLKKPMFPLLEIYYVVSGRIRRRIEDRDRPFIKCNDSGVRIVEVECEKTIDEWLSIDDD 120

Query: 121 VISHRDGGLVHSRAIGPDLGFSPLVFIQLTRFKCGGLAVGLSWAHVLGDIFSASTFINTL 180
            ISHRDG LV+SRAIGPDLGFSPLVFIQLTRF+CGGL+VGLSWAHVLGDIFSASTFIN  
Sbjct: 121 KISHRDGDLVYSRAIGPDLGFSPLVFIQLTRFRCGGLSVGLSWAHVLGDIFSASTFINAW 180

Query: 181 GHIMKNRPVDQLRP-PAVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMATRSFR 240
           GH+MKNRPV++LRP PA AA LIRPS STGRST P+KRLDSTGDLWI S+D KMA RSFR
Sbjct: 181 GHVMKNRPVERLRPLPATAADLIRPSGSTGRSTSPIKRLDSTGDLWIASNDCKMAKRSFR 240

Query: 241 ITEEQLDRIFSVVGQNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNREG 300
           I EEQL RI SVV +NR+AN+S+FEAIAAIFWK LSKIRLEDSDSKTISIYST CP+REG
Sbjct: 241 IMEEQLVRILSVVDRNRSANYSSFEAIAAIFWKCLSKIRLEDSDSKTISIYSTKCPDREG 300

Query: 301 ESPRNGMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGAR 360
           + PRNGMVMSAVEADFPVAGAEEGALAE+I+KKKIDE  EI ELVEKEREESDFIAYGAR
Sbjct: 301 QIPRNGMVMSAVEADFPVAGAEEGALAELIVKKKIDEDEEIKELVEKEREESDFIAYGAR 360

Query: 361 LTFVDLEEANLYGFELEGQKPVHVNYETGGVGENGAVLVLPGPPRGDGRDGGGRTVTVIL 420
           LTFVDLEEANLYG ELEGQ+PVHVNYE GGVGENG VLVLPGPPR DG  GGGRTVTVIL
Sbjct: 361 LTFVDLEEANLYGLELEGQRPVHVNYEIGGVGENGVVLVLPGPPRCDGTPGGGRTVTVIL 420

Query: 421 PEKQLPDLIDELQKQWAIV 439
           PEKQLPDLIDELQKQWAIV
Sbjct: 421 PEKQLPDLIDELQKQWAIV 439

BLAST of Lag0037422 vs. ExPASy TrEMBL
Match: A0A6J1FIP6 (protein ECERIFERUM 2 OS=Cucurbita moschata OX=3662 GN=LOC111445827 PE=3 SV=1)

HSP 1 Score: 750.4 bits (1936), Expect = 4.4e-213
Identity = 379/439 (86.33%), Postives = 404/439 (92.03%), Query Frame = 0

Query: 1   MDGGGNSLISGLKFSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTI 60
           MDG GN LIS +KFSSVVPAKATGENKV EL+AIDLAMKLHYIRGVYFFRPSEEVRNLTI
Sbjct: 1   MDGSGNELISEVKFSSVVPAKATGENKVHELSAIDLAMKLHYIRGVYFFRPSEEVRNLTI 60

Query: 61  YDLKKPMFLLLEPYYVVSGRIRRRIEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDD 120
           YDLKKPMF LLE YYVVSGRIRRRIED DRPFIKCNDSGVRIVE +CEKTIDEWLSI+DD
Sbjct: 61  YDLKKPMFPLLEIYYVVSGRIRRRIEDRDRPFIKCNDSGVRIVEVECEKTIDEWLSIDDD 120

Query: 121 VISHRDGGLVHSRAIGPDLGFSPLVFIQLTRFKCGGLAVGLSWAHVLGDIFSASTFINTL 180
            ISHRDG LV+SRAIGPDLGFSPLVFIQLTRF+CGGL+VGLSWAHVLGDIFSASTFIN  
Sbjct: 121 KISHRDGDLVYSRAIGPDLGFSPLVFIQLTRFRCGGLSVGLSWAHVLGDIFSASTFINAW 180

Query: 181 GHIMKNRPVDQLRP-PAVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMATRSFR 240
           GH+MKNRPV++LRP PA AA LIRPS STGRST P+KRLDSTGDLWI S+D KMA RSFR
Sbjct: 181 GHVMKNRPVERLRPLPATAADLIRPSGSTGRSTSPIKRLDSTGDLWIASNDCKMAKRSFR 240

Query: 241 ITEEQLDRIFSVVGQNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNREG 300
           ITEE+L RI SVV +NR+AN+S+FEAIAAIFWK LSKIRLEDSDSKTISIYST CP+REG
Sbjct: 241 ITEEKLVRILSVVDRNRSANYSSFEAIAAIFWKCLSKIRLEDSDSKTISIYSTKCPDREG 300

Query: 301 ESPRNGMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGAR 360
           + P NGMVMSA+EADFPVAGAEEGALAE+I+KKKIDEG EI ELVEKERE+SDFIAYGAR
Sbjct: 301 QIPINGMVMSAIEADFPVAGAEEGALAELIVKKKIDEGEEIKELVEKEREDSDFIAYGAR 360

Query: 361 LTFVDLEEANLYGFELEGQKPVHVNYETGGVGENGAVLVLPGPPRGDGRDGGGRTVTVIL 420
           LTFVDLEEANLYG ELEGQ+PVHVNYE GGVGENG VLVLPGPPR DGR GG RTVTVIL
Sbjct: 361 LTFVDLEEANLYGLELEGQRPVHVNYEIGGVGENGVVLVLPGPPRCDGRPGGDRTVTVIL 420

Query: 421 PEKQLPDLIDELQKQWAIV 439
           PEKQLP+LIDELQKQWAIV
Sbjct: 421 PEKQLPELIDELQKQWAIV 439

BLAST of Lag0037422 vs. ExPASy TrEMBL
Match: A0A6J1C6H6 (protein ECERIFERUM 2 OS=Momordica charantia OX=3673 GN=LOC111008780 PE=3 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 2.0e-194
Identity = 354/440 (80.45%), Postives = 383/440 (87.05%), Query Frame = 0

Query: 1   MDGGGNSLISGLKFSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTI 60
           MDG  +SLISG+KFSSVVPAKA+GENKVQELTA+DL MKLHYIRGVYFFRPSEE+RNLTI
Sbjct: 1   MDGASDSLISGVKFSSVVPAKASGENKVQELTAMDLVMKLHYIRGVYFFRPSEEIRNLTI 60

Query: 61  YDLKKPMFLLLEPYYVVSGRIRRRIE-DGDRPFIKCNDSGVRIVEADCEKTIDEWLSIED 120
           YDLKKPMF LLE Y+VVSGRIRRRIE  G+RPFIKCNDSGVRIVEADC+K+IDEWLS+ED
Sbjct: 61  YDLKKPMFPLLELYFVVSGRIRRRIEAGGNRPFIKCNDSGVRIVEADCQKSIDEWLSMED 120

Query: 121 DVISHRDGGLVHSRAIGPDLGFSPLVFIQLTRFKCGGLAVGLSWAHVLGDIFSASTFINT 180
           +  SHRD GLVHSRA+GPDLGFSPLVFIQLTRFKCGGLAVGLSWAHVLGDIFSAS FIN 
Sbjct: 121 EKYSHRDHGLVHSRALGPDLGFSPLVFIQLTRFKCGGLAVGLSWAHVLGDIFSASAFINV 180

Query: 181 LGHIMKN-RPVDQLRPPAVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMATRSF 240
            GHIM N RPV QLR       + RPS ST ++  PVKRLD TGDLWIGSSD KMATRSF
Sbjct: 181 WGHIMNNRRPVCQLR--THTPTICRPSGSTTQTRSPVKRLDQTGDLWIGSSDCKMATRSF 240

Query: 241 RITEEQLDRIFSVVGQNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNRE 300
           RI EEQL+RI S+V +NRA  FSNFEA+AAIFWKSLSKIR  DSD KTISIYST CPNRE
Sbjct: 241 RIMEEQLERILSIVRRNRAVAFSNFEALAAIFWKSLSKIR-PDSDLKTISIYSTKCPNRE 300

Query: 301 GESPRNGMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGA 360
           GE P NGMVMSAV+AD PV GAEEGALAE+I++K+IDEGGEI ELVEKE EESDFIAYG 
Sbjct: 301 GEIPMNGMVMSAVKADAPVTGAEEGALAELILEKRIDEGGEIEELVEKEMEESDFIAYGG 360

Query: 361 RLTFVDLEEANLYGFELEGQKPVHVNYETGGVGENGAVLVLPGPPRGDGRDGGGRTVTVI 420
           RLTFVDLEEAN+YG EL GQKPVH NYE GGVG++G VLVLPGPPR  G  GGGR VTVI
Sbjct: 361 RLTFVDLEEANIYGLELGGQKPVHANYEIGGVGDDGVVLVLPGPPRDGGGYGGGRNVTVI 420

Query: 421 LPEKQLPDLIDELQKQWAIV 439
           +PEKQLP LIDELQKQWAI+
Sbjct: 421 IPEKQLPALIDELQKQWAII 437

BLAST of Lag0037422 vs. ExPASy TrEMBL
Match: A0A0A0LEQ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G892170 PE=3 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 8.5e-193
Identity = 346/434 (79.72%), Postives = 381/434 (87.79%), Query Frame = 0

Query: 9   ISGLKFSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTIYDLKKPMF 68
           +S L+FSSVVPAKATGE++V+ELTA+DLAMKLHYIRGVYFFR SEEVRNLT+YDLKKP+F
Sbjct: 10  VSDLRFSSVVPAKATGEDEVKELTAMDLAMKLHYIRGVYFFRASEEVRNLTVYDLKKPLF 69

Query: 69  LLLEPYYVVSGRIRRRI--EDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIED-DVISHR 128
           LLLE YYVVSGRIRRRI  +DGDR FIKCNDSGVRIVEADCEKTI+EWLSIED D I +R
Sbjct: 70  LLLEKYYVVSGRIRRRIVGDDGDRAFIKCNDSGVRIVEADCEKTIEEWLSIEDGDKILNR 129

Query: 129 DGGLVHSRAIGPDLGFSPLVFIQLTRFKCGGLAVGLSWAHVLGDIFSASTFINTLGHIMK 188
           DG LVHS+AIGPDLGFSPL FIQLTRFKCGGL+VGLSW HVLGDIFSASTFIN  GHIM 
Sbjct: 130 DGCLVHSQAIGPDLGFSPLAFIQLTRFKCGGLSVGLSWTHVLGDIFSASTFINVWGHIMN 189

Query: 189 NRPVDQLRP-PAVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMATRSFRITEEQ 248
           NRP+ QLRP PA   P  R SR    ST P+KRLD TGDLWIGS+D KMATRSFRIT  Q
Sbjct: 190 NRPLHQLRPAPATHIPTSRSSRLI--STPPLKRLDPTGDLWIGSTDCKMATRSFRITSAQ 249

Query: 249 LDRIFSVVGQNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNREGESPRN 308
           LDRI SVVG+NR+ NFS FE+I A+FW SLSKIRLEDSDS+TISIYST CPNRE E P N
Sbjct: 250 LDRITSVVGRNRSMNFSTFESITAVFWNSLSKIRLEDSDSRTISIYSTKCPNRESEIPSN 309

Query: 309 GMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGARLTFVD 368
           GM MS VEADFPVAGA EG LAE+I+KK+IDEGGE+ ELVE + ++SDFIAYGARLTFVD
Sbjct: 310 GMEMSGVEADFPVAGAAEGELAEVIVKKRIDEGGEVEELVETKMDDSDFIAYGARLTFVD 369

Query: 369 LEEANLYGFELEGQKPVHVNYETGGVGENGAVLVLPGPPRGDGRDGGGRTVTVILPEKQL 428
           LEEA++YGFELEGQK VHVNYE GGVGENG V+VLPGPPR DGRDGGGRTVTVILPEK++
Sbjct: 370 LEEADVYGFELEGQKAVHVNYEIGGVGENGVVMVLPGPPRSDGRDGGGRTVTVILPEKEV 429

Query: 429 PDLIDELQKQWAIV 439
           PDLIDE++KQW IV
Sbjct: 430 PDLIDEMEKQWGIV 441

BLAST of Lag0037422 vs. ExPASy TrEMBL
Match: A0A1S3CQJ9 (protein ECERIFERUM 1 OS=Cucumis melo OX=3656 GN=LOC103503654 PE=3 SV=1)

HSP 1 Score: 680.2 bits (1754), Expect = 5.5e-192
Identity = 354/446 (79.37%), Postives = 383/446 (85.87%), Query Frame = 0

Query: 1   MDGGGNSL-ISGLKFSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLT 60
           MDG   +  IS LKFSSVVPAKATGE++V+ELTA+DLAMKLHYIRGVYFFR SEEV+NLT
Sbjct: 1   MDGVAKTTPISDLKFSSVVPAKATGEDEVKELTAMDLAMKLHYIRGVYFFRASEEVKNLT 60

Query: 61  IYDLKKPMFLLLEPYYVVSGRIRRRI---EDGDRPFIKCNDSGVRIVEADCEKTIDEWLS 120
           IYDLKKP+FLLLE YYVVSGRIRRRI    DGDR FIKCNDSGVRIVEADCEKTI+EWLS
Sbjct: 61  IYDLKKPLFLLLEKYYVVSGRIRRRIIGDNDGDRAFIKCNDSGVRIVEADCEKTIEEWLS 120

Query: 121 IE---DDVISHRDGGLVHSRAIGPDLGFSPLVFIQLTRFKCGGLAVGLSWAHVLGDIFSA 180
           IE   DD I +RDG LVHS+AIGPDLGFSPL FIQLTRFKCGGL+VGLSW HVLGDIFSA
Sbjct: 121 IEDDDDDKILNRDGCLVHSQAIGPDLGFSPLAFIQLTRFKCGGLSVGLSWTHVLGDIFSA 180

Query: 181 STFINTLGHIMKNRPVDQLRP-PAVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFK 240
           STFIN  GHIM NRP+ QLRP PA   P    SR    ST P+KRLD TGDLWIGS+D K
Sbjct: 181 STFINVWGHIMNNRPLHQLRPAPATHIPPSTSSRLI--STPPLKRLDPTGDLWIGSTDCK 240

Query: 241 MATRSFRITEEQLDRIFSVVGQNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYST 300
           MATRSFRIT EQLDRI SVVG+NR+ NFS FE+I A+FWKSLSKIRLEDS S+TISIYST
Sbjct: 241 MATRSFRITSEQLDRITSVVGRNRSVNFSTFESITAVFWKSLSKIRLEDSGSRTISIYST 300

Query: 301 NCPNREGESPRNGMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESD 360
             PNREGE P NGM MS VEADFPVAGA EG LAE+I+KKKIDEGGEI ELVE +  +SD
Sbjct: 301 KFPNREGEIPSNGMEMSGVEADFPVAGAAEGELAEVIVKKKIDEGGEIEELVETKMNDSD 360

Query: 361 FIAYGARLTFVDLEEANLYGFELEGQKPVHVNYETGGVGENGAVLVLPGPPRGDGRDGGG 420
           FIAYGARLTFVDLEEA++YGFELEGQK VHVNYE GGVGENG VLVLPGPPR DGRDGGG
Sbjct: 361 FIAYGARLTFVDLEEADIYGFELEGQKAVHVNYEIGGVGENGVVLVLPGPPRSDGRDGGG 420

Query: 421 RTVTVILPEKQLPDLIDELQKQWAIV 439
           RTVTVILPEK+LP+LIDE++KQW IV
Sbjct: 421 RTVTVILPEKELPELIDEMEKQWGIV 444

BLAST of Lag0037422 vs. TAIR 10
Match: AT4G24510.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 306.2 bits (783), Expect = 4.2e-83
Identity = 175/435 (40.23%), Postives = 262/435 (60.23%), Query Frame = 0

Query: 7   SLISGLKFSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTIYDLKKP 66
           S ++ ++ SSVVPA   GENK ++LT +DLAMKLHY+R VYFF+ +   R+ T+ D+K  
Sbjct: 4   SPVTSVRLSSVVPASVVGENKPRQLTPMDLAMKLHYVRAVYFFKGA---RDFTVADVKNT 63

Query: 67  MFL---LLEPYYVVSGRIRRRIEDGDR-----PFIKCNDSGVRIVEADCEK-TIDEWLSI 126
           MF    LL+ Y+ VSGRIR    D D      P+I+CNDSG+R+VEA+ E+ T+++WL +
Sbjct: 64  MFTLQSLLQSYHHVSGRIRMSDNDNDTSAAAIPYIRCNDSGIRVVEANVEEFTVEKWLEL 123

Query: 127 EDDVISHRDGGLVHSRAIGPDLGFSPLVFIQLTRFKCGGLAVGLSWAHVLGDIFSASTFI 186
           +D  I HR   LV+   +GPDL FSPLVF+Q+T+FKCGGL +GLSWAH+LGD+FSASTF+
Sbjct: 124 DDRSIDHR--FLVYDHVLGPDLTFSPLVFLQITQFKCGGLCIGLSWAHILGDVFSASTFM 183

Query: 187 NTLGHIMKNRPVDQLRPPAVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMATRS 246
            TLG ++        +P     P +          + ++++DS G+ W+ ++  KM    
Sbjct: 184 KTLGQLVSGHA--PTKPVYPKTPELTSHARNDGEAISIEKIDSVGEYWLLTNKCKMGRHI 243

Query: 247 FRITEEQLDRIFSVVGQNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNR 306
           F  +   +D + +     R   FS  + + A+ WKSL  IR E +++  I+I    C  +
Sbjct: 244 FNFSLNHIDSLMAKY-TTRDQPFSEVDILYALIWKSLLNIRGE-TNTNVITI----CDRK 303

Query: 307 EGESPRN-GMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAY 366
           +  +  N  +V+S VE +  + G  E  LA +I  +K +E G I  ++E+++  SDF  Y
Sbjct: 304 KSSTCWNEDLVISVVEKNDEMVGISE--LAALIAGEKREENGAIKRMIEQDKGSSDFFTY 363

Query: 367 GARLTFVDLEEANLYGFELEGQKPVHVNYETGGVGENGAVLVLPGPPRGDGRDGGGRTVT 426
           GA LTFV+L+E ++Y  E+ G KP  VNY   GVG+ G VLV P       +    R V+
Sbjct: 364 GANLTFVNLDEIDMYELEINGGKPDFVNYTIHGVGDKGVVLVFP-------KQNFARIVS 416

Query: 427 VILPEKQLPDLIDEL 432
           V++PE+ L  L +E+
Sbjct: 424 VVMPEEDLAKLKEEV 416

BLAST of Lag0037422 vs. TAIR 10
Match: AT3G23840.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 262.7 bits (670), Expect = 5.3e-70
Identity = 157/429 (36.60%), Postives = 237/429 (55.24%), Query Frame = 0

Query: 5   GNSLISGLKFSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTIYDLK 64
           G+  + G + S+V  +  +      E T +DLAMKLHY++ VY +  +   R+LT+ D+K
Sbjct: 8   GSGPVHGFRLSTVSASLPSETGTTHEPTGLDLAMKLHYLKAVYIY-SAGTARDLTVMDVK 67

Query: 65  KPMFLLLEPYYVVSGRIRRRIEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDDVISH 124
            P+F +      + GR RR   +  RP++KCND G R VE+ C+ T++EWL + D  +  
Sbjct: 68  APLFSVFYQIPCIIGRFRR--HESGRPYLKCNDCGTRFVESHCDLTVEEWLRVPDRSV-- 127

Query: 125 RDGGLVHSRAIGPDLGFSPLVFIQLTRFKCGGLAVGLSWAHVLGDIFSASTFINTLGHIM 184
            D  LV+ + +GPDL FSPL++IQ+TRF CGGLA+GLSWAH++GD FS S F N      
Sbjct: 128 -DESLVYHQPVGPDLAFSPLLYIQMTRFSCGGLALGLSWAHIMGDPFSLSHFFNLWAQAF 187

Query: 185 KNRPVDQLRPPAVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMATRSFRITEEQ 244
               +   +          P+ ST +    VK++D  GDLW+  ++ KM T SF +T   
Sbjct: 188 AGGKIYCPKTSVTERDFQNPT-STFKKPDSVKQVDLVGDLWVAPNNSKMTTFSFNLTVND 247

Query: 245 LDRIFSVVGQNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNREGESPRN 304
           L   F V G         FE +  I WK ++ +R E S   TI++  ++    +  + RN
Sbjct: 248 LKTHFPVNGD------GEFEILTGIIWKCVATVRGE-SAPVTITVIRSDPKKLKPRAVRN 307

Query: 305 GMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGARLTFVD 364
           G ++S++  DF VA A    + + I + K DE   I E+V+   + SDFI YGA LTFVD
Sbjct: 308 GQMISSIHVDFSVAEASLEEIVKSIGEAK-DERVVIDEIVD---DVSDFIVYGANLTFVD 367

Query: 365 LEEANLYGFELEGQKPVHVNYETGGVGENGAVLVLPGPPRGDGRDGGGRTVTVILPEKQL 424
           + E + Y  ++ G+ P  V     G+G++GAV+VLPG    +      R VTV LP  ++
Sbjct: 368 MSEVDFYEAKVMGKSPESVYCNVQGIGDDGAVVVLPGVVEEE------RVVTVTLPVDEI 412

Query: 425 PDLIDELQK 434
             +  E++K
Sbjct: 428 EKVKWEMKK 412

BLAST of Lag0037422 vs. TAIR 10
Match: AT4G13840.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 252.3 bits (643), Expect = 7.2e-67
Identity = 152/432 (35.19%), Postives = 233/432 (53.94%), Query Frame = 0

Query: 3   GGGNSLISGLKFSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTIYD 62
           G G   +  ++ S+V   + T      E T +DLAMKLHY++  Y +  +E  R+LT+  
Sbjct: 8   GQGQGPVHSIRLSTVGATRPTETGTTHEPTGLDLAMKLHYLKAAYIY-SAETARDLTVRH 67

Query: 63  LKKPMFLLLEPYYVVSGRIRRRIEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDDVI 122
           LK+ MF+L +     +GR  RR  D  RP+IKCND G R VE  C  T++EWLS  D  +
Sbjct: 68  LKEAMFMLFDQIAWTTGRFSRR--DSGRPYIKCNDCGTRFVEGQCNLTVEEWLSKPDRSV 127

Query: 123 SHRDGGLVHSRAIGPDLGFSPLVFIQLTRFKCGGLAVGLSWAHVLGDIFSASTFINTLGH 182
              D  LV+   IGP+L FSPL+++Q+TRFKCGGL +GLSWA+++GD FS     N    
Sbjct: 128 ---DEFLVYHHPIGPELTFSPLIYVQMTRFKCGGLGLGLSWANIIGDAFSLFYAFNLWAK 187

Query: 183 IMKNRPVDQLRPPAVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMATRSFRIT- 242
            +    +     P++     +    T +  + +KR++  GDLW+  +D K+A   F ++ 
Sbjct: 188 AITGEKIYAPTTPSIGERRFQSPNPTVKDPVSIKRVEPVGDLWVTPNDKKLANYCFNLSV 247

Query: 243 EEQLDRIFSVVGQNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNREGES 302
            +Q+   F   G +   +   FE +A I WK ++K+R+E     T++I   +  + +  +
Sbjct: 248 ADQISPHFPAKGDD---SIPVFEILAGIIWKCIAKVRVEPK-PVTVTIIKKDPNDLKLNA 307

Query: 303 PRNGMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGARLT 362
            RN  V+S+V  DFPVA A    L + + + K DE   I E+ E      DF+ YGA+LT
Sbjct: 308 IRNSQVISSVSVDFPVAEATVEELVKAMGEAK-DERCGIEEIGESCDGNLDFVVYGAKLT 367

Query: 363 FVDLEEANLYGFELEGQKPVHVNYETGGVGENGAVLVLPGPPRGDGRDGGGRTVTVILPE 422
           F+DL   +LY  ++ G+ P  V     G+GE G V+V       +      R VTV LPE
Sbjct: 368 FLDLTGEDLYEAKVMGKSPESVYCNVEGIGEEGLVVVYAAAKSEE------RVVTVTLPE 422

Query: 423 KQLPDLIDELQK 434
           +++  +  E +K
Sbjct: 428 EEMERVKLEFKK 422

BLAST of Lag0037422 vs. TAIR 10
Match: AT4G29250.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 96.7 bits (239), Expect = 5.0e-20
Identity = 116/454 (25.55%), Postives = 201/454 (44.27%), Query Frame = 0

Query: 16  SVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSE--EVRNLTIYDLKKPMFLLLEP 75
           +VV  ++    ++ +L+ +D  M+ ++IR VY++R S+  E   +T   L++ +   L  
Sbjct: 21  TVVSTRSIEPGRLYQLSVLDHVMEPNHIRLVYYYRCSKTREPGEIT-KKLRESLAYTLNC 80

Query: 76  YYVVSGRIRRRIED-------GDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDDVISHRD 135
           Y +V+GR+ + ++          R  +K ND+GVR+VEA    ++ EWL     V    +
Sbjct: 81  YPIVTGRLVKEVDGMEENKDLSQRWMVKSNDAGVRMVEARATGSVKEWLR---SVNREEE 140

Query: 136 GGLVHSRAIGPDLGFSPLVFIQLTRFKCGGLAVGLSWAHVLGDIFSASTFINTLGHIMKN 195
             LVH   +     +     +Q+T F+ GGLA+GLS +H+L D   A  FI     +  +
Sbjct: 141 LKLVHWEDMYHLQYYWSTFCVQVTEFESGGLAIGLSCSHLLADPVCAMMFIRAWADLTLS 200

Query: 196 RPVDQLRPPAVAAPLIRPSRSTGRSTLPVKRLDSTGDL---WIGSSDFKMATRSFRITEE 255
           R +       +A PL  P      S    +RL S   L   +I S    +      +TE+
Sbjct: 201 RSM-------MAPPLFHPLPPRRFSN---QRLISNNQLLSHYIKSC--SLTASPSNMTED 260

Query: 256 QLDRIFSV-------VGQNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISI------- 315
            +  +  +        G+N     S FE +A +FW  +S+ + + ++   +S+       
Sbjct: 261 HMVTVTFLFPDPLVRAGENE-PRISTFEILAGLFWVCVSRAKGKRNELMDMSLCLDVRKL 320

Query: 316 ------YSTNCPNREGESPRNGMVMSAVEADFPVAGAEE---GALAEMIMKKKIDEGGEI 375
                 Y  NC           MV   V    PV   ++    A+ E+    K  +   +
Sbjct: 321 LRLDQSYFGNC-----------MVYHKVPYSKPVKTKDKLLFHAVQEIESITKRLDYDTV 380

Query: 376 VELVEKEREESDFIAYGARLTFVDLE---EANLYGFELEGQKPVHVN-YETGGVGENGAV 431
           ++L+E     +  I+ G+ L   +LE    +    FE E     HV+ Y  G V   G V
Sbjct: 381 MDLIEWLSSNNGAISNGSDLVCTNLENMSHSRPMMFE-EDLALSHVSCYVEGPVAGGGQV 440

BLAST of Lag0037422 vs. TAIR 10
Match: AT5G48930.1 (hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase )

HSP 1 Score: 86.7 bits (213), Expect = 5.2e-17
Identity = 73/278 (26.26%), Postives = 128/278 (46.04%), Query Frame = 0

Query: 34  IDLAMKLHYIRGVYFFRPSEEVRNLTIYDLKKPMFLLLEPYYVVSGRIRRRIEDGDRPFI 93
           +DL +   +   VYF+RP+          +K+ +   L P+Y ++GR++R  +D  R  I
Sbjct: 27  VDLVIPRFHTPSVYFYRPTGASNFFDPQVMKEALSKALVPFYPMAGRLKR--DDDGRIEI 86

Query: 94  KCNDSGVRIVEADCEKTIDEW------LSIEDDV--ISHRDGGLVHSRAIGPDLGFSPLV 153
            CN +GV  V AD    ID++      L++   +  + H  G  +HS          PL+
Sbjct: 87  DCNGAGVLFVVADTPSVIDDFGDFAPTLNLRQLIPEVDHSAG--IHS---------FPLL 146

Query: 154 FIQLTRFKCGGLAVGLSWAHVLGDIFSASTFINTLGHIMKNRPVDQLRPPAVAAPLIRPS 213
            +Q+T FKCGG ++G+   H   D FS   FINT   +   R +D   PP +   L+R +
Sbjct: 147 VLQVTFFKCGGASLGVGMQHHAADGFSGLHFINTWSDMA--RGLDLTIPPFIDRTLLR-A 206

Query: 214 RSTGRSTLPVKRLDSTGDLWIGSSDFKMATRS-----FRITEEQLDRIFSVVGQN-RAAN 273
           R   +             + I     K    +     F++T +QL  + +   ++    +
Sbjct: 207 RDPPQPAFHHVEYQPAPSMKIPLDPSKSGPENTTVSIFKLTRDQLVALKAKSKEDGNTVS 266

Query: 274 FSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNR 298
           +S++E +A   W+S+ K R   +D +T    +T+  +R
Sbjct: 267 YSSYEMLAGHVWRSVGKARGLPNDQETKLYIATDGRSR 288

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022980996.11.1e-21387.02protein ECERIFERUM 2 [Cucurbita maxima][more]
KAG6608131.14.0e-21386.10Protein ECERIFERUM 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022940107.19.0e-21386.33protein ECERIFERUM 2 [Cucurbita moschata] >KAG7031769.1 Protein ECERIFERUM 2 [Cu... [more]
XP_023523388.12.9e-21186.56protein ECERIFERUM 2-like [Cucurbita pepo subsp. pepo][more]
XP_038898516.12.6e-20484.67protein ECERIFERUM 2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q390485.9e-8240.23Protein ECERIFERUM 2 OS=Arabidopsis thaliana OX=3702 GN=CER2 PE=1 SV=1[more]
Q9LIS17.5e-6936.60Protein ECERIFERUM 26-like OS=Arabidopsis thaliana OX=3702 GN=CER26L PE=2 SV=1[more]
Q9SVM91.0e-6535.19Protein ECERIFERUM 26 OS=Arabidopsis thaliana OX=3702 GN=CER26 PE=2 SV=1[more]
A0A2H5AIZ19.2e-1927.41Hydroxycinnamoyltransferase OS=Narcissus pseudonarcissus OX=39639 GN=HCT PE=2 SV... [more]
Q9FI787.3e-1626.26Shikimate O-hydroxycinnamoyltransferase OS=Arabidopsis thaliana OX=3702 GN=HST P... [more]
Match NameE-valueIdentityDescription
A0A6J1IV895.1e-21487.02protein ECERIFERUM 2 OS=Cucurbita maxima OX=3661 GN=LOC111480277 PE=3 SV=1[more]
A0A6J1FIP64.4e-21386.33protein ECERIFERUM 2 OS=Cucurbita moschata OX=3662 GN=LOC111445827 PE=3 SV=1[more]
A0A6J1C6H62.0e-19480.45protein ECERIFERUM 2 OS=Momordica charantia OX=3673 GN=LOC111008780 PE=3 SV=1[more]
A0A0A0LEQ18.5e-19379.72Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G892170 PE=3 SV=1[more]
A0A1S3CQJ95.5e-19279.37protein ECERIFERUM 1 OS=Cucumis melo OX=3656 GN=LOC103503654 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G24510.14.2e-8340.23HXXXD-type acyl-transferase family protein [more]
AT3G23840.15.3e-7036.60HXXXD-type acyl-transferase family protein [more]
AT4G13840.17.2e-6735.19HXXXD-type acyl-transferase family protein [more]
AT4G29250.15.0e-2025.55HXXXD-type acyl-transferase family protein [more]
AT5G48930.15.2e-1726.26hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 229..429
e-value: 1.6E-13
score: 52.3
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 9..217
e-value: 1.2E-38
score: 134.7
IPR003480TransferasePFAMPF02458Transferasecoord: 16..425
e-value: 2.5E-32
score: 112.1
NoneNo IPR availablePANTHERPTHR31642:SF259PROTEIN ECERIFERUM 2coord: 8..435
NoneNo IPR availablePANTHERPTHR31642TRICHOTHECENE 3-O-ACETYLTRANSFERASEcoord: 8..435

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0037422.1Lag0037422.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups