Lag0037419 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0037419
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionArgininosuccinate lyase
Locationchr2: 6052929 .. 6056610 (-)
RNA-Seq ExpressionLag0037419
SyntenyLag0037419
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTATGACGTATGTGTCAACAAAATATCATATGACACTACACACTTAAGAGCTCCAACTCGCCGACGACGCGCCGCCAGTCGTCAGAATTTCCAGCCGCCGAAGATGGATTGCTTGGCCCTCCATTCCTCTTTACCCGCCGCATTTGCCTTCTCTAAGCCCTCCGTTTCAAGCTCTCAGCCGCCATTTCTCGCCGTCGCTCGTGGAGCCATGCCGGTGTCTGGGGTTCTCAAAGCATCTCGCACTGCCGATGTGGCGCCGTCCGTCGCCGACTCGCCGGAGAACCGAACCGACAAGGAGGTTAAGCTTTGGGGCGGTCGTTTCGAGGAGAGCGTCACCGATGCAGTTGAGCGATTCACCGAATCGATCTCCTACGATAAGCAGCTTTACAAGCACGATATCAGAGGAAGTCGAGCTCATGCTACTATGCTAGCTAAGCAGGTTCGTTTCCATTCCTCTACTTCTCTGCGTAAACAAAATCGCATTCGTTTTTCTCTTTATGAGCGTTGCATTTCGGTCGACTTCACTACTGTTTGGCAGAAGAATAGAAATTAATTGACATCGCTGTTGTCAGGTAATTTCATCTTTTAATTTCTTTATCGAGAAGTGTAATTCTAGTGGATACATTGATTTAAGATTGCGGATTACATTTAAACTCCTTAATTTTGTTGTAGTTTACCAGTTGGCTTGTTGGTTGAATCTTTGTCCATCCTGGTTTAGATGAATTCAGAAATATGTATCATACGGTGTTATTATATTCATGGATGTTTTCCTTTTCGTGTCTTTAATGATTGGAAACTTAATACTTTAGGGACTGATGAGTGTTGATGAAAGGGACAGTATTCTTGAAGGTTTGGATGAAATTGAGAGGCGCATAGAAAGTGGTGATTTTGTTTGGAGAACAGATAGGGAGGATGTTCATATGAACATTGAGGCTGCCTTGATTGATATGATTGGTGAGCCTGCAAAGAAGCTACATACCGCTCGAAGCCGCAACGATCAAGTTCTGACTGATTTTCGATTGTGGTGTCGGGATGCAATTGATAATATATTAGATGCTATTAAGTATCTTCAGGTTTCCATGGTAACTCTGGCTTTGAAGAATGAGGGAGTAATCGTTCCGGGCTATACACATTTACAACGAGCGCAGCCCGTTCTGCTTCAGCATCTGCTCTTAGCATTTGTTGAGCAGGTAAGCTTCTTGAAGTGAAGTTCCTCTGTCTTCCTTCATTCAAGCCTAAGAGTTTTCAACTTATCAGGATAAGCAATTGGCAATATCTACATGCATGATTATTTTATATTCGTTTTCAATGCTTGTACTTTTTAGGAATCAGGATTATTCCATCAGGATTTTCTTGAAATCTCATTCAATTTATCGATAGTGTTATTGACAGCTTGAGTGTTCATTATAACTTCATTTTTTAAAGTCAGAAATAGACATTTTTGTTAAAATAAAAGTACTTTTAGCTTATATAATATTCACATATGGTTGTTGTGATTCTATATATCATTTTTTTAAAAGCCGTGTGAGCTCATGCAATCTCAAGTGCACATTAAGAACATTTGGATTTACATGTTGCCCCTTTGACTAATGGACAAAACAATGGTGGTTATTTGATGTCACTTTGATTGAAAAATTAGTATTTTTTTAATGATCTGTTGGCAAATGGATCAATAGTTTATCGTTAAATTTGCATAACAATCAACCTTACTCATCGTTTCTTAAATAAAATGTGGTTTTAAGCACTTTATTATTTTTTTCTCTCTGAACTTAAAATCTCTAGAAGACTTGATTGTTGTAAAATCATTGTTTTATAGCTTGAAAGAGATGCTGGACGTTTGTCGGATTGCAGAGTTAGGCTAAATCTTTGCCCGCTAGGTGCATGTGCGCTAGCCGGCACAGGCCTTCCAATTGATCGTTTTATGACTGCTGAGGCTTTAGGGTTCACCGGTCCCTTGAGAAACAGGTATAAAACTTCATTTATTTCTCTCCTTTATTTATTTATTTATTTTTATTTAAAAGCTGTAAGAGTTGCTAAAGATCGTCTACCTGTTTGACTTGCTATTTAAAGTTCTTCTTGAGATATGTGTGTATGTGTGGTAGTTTATCCGTATAAATAACAAAGGGCTTAGAAGGAATAGGTTCAAGCCATGGTGGCCACCTACCTAAGATTTAATATCTTATGAATTAACTATTTACCTTGACAACCGAATGTAGTAGGGCCAAATGGTTATTCCATTAGAATAGTTGAGGTGCGAGCAAGCTGGTTCAAACACTCACCGATATAAAATATATATATATATATACACACAACACGTGAATGTATATATGCATATATAGCTTTTTTTTTCGTCTCATTCACTTCAATAATCTTTAGCTGATTAAGCTTTCACTTTCTGTGGGGAGTTTTAATGACTTCTATTTCCACGTCTGATTGTGTCAAATCATTAGAAATTTATCAGTATCTTTACAGCATTGATGCAGTTTCAGATCGAGATTTTGCCTTGGAGTTTCTTTCTGCCAATGCCATTACGGCTATTCATCTTTCAAGGCTTGGAGAAGAATGGGTGTTGTGGGCTTCTGAAGAATTCGGATTTATAACGCCAAATGATTCTGTTTCAACTGGAAGTAGCATAATGCCTCAGAAGAAAAATCCAGATCCAATGGAACTCGTTCGTGGAAAATCCGCTAGAGTCATAGGGGACTTGGTAACCACTCTCACTTTATGCAAAGGTCTGCCTCTGGCCTACAATCGTGATTTGCAGGTAAATCTTTGTGCATTTTGTTTTGGCCAATTTGTTAGATGCATAACAAAGGGTTGACACTTCCAAAGTGAATTGTGTAGACTTAACACAACCCGAGAGTGTTTTTTCCATTTAGGCAATTTTAATTCCCAGACAAATGCTTCACTTTACTTTTTCTTATCGATGTCGTTTTTAGTTCTACTTCTCGTTAATACAAAAATGTTACGATATTGAACAGGAAGACAAGGAACCTGTATTTGACAGCGTAAAAACGATATTGGGGATGCTGGAAGTGTCAGCTGAGTTTGCTCAGAATATTTCTTTCAATCGGGAGAGAATACAAAAGGCTTTGCCTGCTGGTTATCTTGATGCCACAACACTTGCCGATTACCTCGTGAAGAAGGTAACTAAATTCCTATTGGTTTGATTGCAATTTTTTTTTCTTTTTCAAAAACTGTCTTAGTTCTTGTGATTAATATAAGGAAAGCAACATCGATTCGTCTGTTATTGCACTACGTAGTATATTGATATTAGCCAAAGACGACCTCGTTTTAATACTGCTTAGGCGTTCGAGACCTCTATTGTAAAACTCTTTCCACTATGGAAGTAAAAACAGAAGAAGAAGAAATCAAATTCTATAAATTCAATGACTTAACAAGGCTTCTATTTGTCTTTCCTTTTGCAGGGAATACCTTTCAGAACAGGTCATGATATAGTTGGGAAGTCCGTTGCCTTGTGTGTCACAAAGAATTGCTGCCTCCAGGACTTGAGTCTGGATGAGCTAAGAAGCATAAGTCCAGTATTTGAAGAGGACGTTTACGAGTTTCTCGGCGCAGAGAATGCGATTAATAAATTTTGTTCATATGGATCGACAGGAGCAGCCTGTGTTGCCACTCAAATCGAATACTGGGTTAATAAACTCCAAATCAGTCTGTAG

mRNA sequence

ATGTATGACGTATGTGTCAACAAAATATCATATGACACTACACACTTAAGAGCTCCAACTCGCCGACGACGCGCCGCCAGTCGTCAGAATTTCCAGCCGCCGAAGATGGATTGCTTGGCCCTCCATTCCTCTTTACCCGCCGCATTTGCCTTCTCTAAGCCCTCCGTTTCAAGCTCTCAGCCGCCATTTCTCGCCGTCGCTCGTGGAGCCATGCCGGTGTCTGGGGTTCTCAAAGCATCTCGCACTGCCGATGTGGCGCCGTCCGTCGCCGACTCGCCGGAGAACCGAACCGACAAGGAGGTTAAGCTTTGGGGCGGTCGTTTCGAGGAGAGCGTCACCGATGCAGTTGAGCGATTCACCGAATCGATCTCCTACGATAAGCAGCTTTACAAGCACGATATCAGAGGAAGTCGAGCTCATGCTACTATGCTAGCTAAGCAGGGACTGATGAGTGTTGATGAAAGGGACAGTATTCTTGAAGGTTTGGATGAAATTGAGAGGCGCATAGAAAGTGGTGATTTTGTTTGGAGAACAGATAGGGAGGATGTTCATATGAACATTGAGGCTGCCTTGATTGATATGATTGGTGAGCCTGCAAAGAAGCTACATACCGCTCGAAGCCGCAACGATCAAGTTCTGACTGATTTTCGATTGTGGTGTCGGGATGCAATTGATAATATATTAGATGCTATTAAGTATCTTCAGGTTTCCATGGTAACTCTGGCTTTGAAGAATGAGGGAGTAATCGTTCCGGGCTATACACATTTACAACGAGCGCAGCCCGTTCTGCTTCAGCATCTGCTCTTAGCATTTGTTGAGCAGCTTGAAAGAGATGCTGGACGTTTGTCGGATTGCAGAGTTAGGCTAAATCTTTGCCCGCTAGGTGCATGTGCGCTAGCCGGCACAGGCCTTCCAATTGATCGTTTTATGACTGCTGAGGCTTTAGGGTTCACCGGTCCCTTGAGAAACAGCATTGATGCAGTTTCAGATCGAGATTTTGCCTTGGAGTTTCTTTCTGCCAATGCCATTACGGCTATTCATCTTTCAAGGCTTGGAGAAGAATGGGTGTTGTGGGCTTCTGAAGAATTCGGATTTATAACGCCAAATGATTCTGTTTCAACTGGAAGTAGCATAATGCCTCAGAAGAAAAATCCAGATCCAATGGAACTCGTTCGTGGAAAATCCGCTAGAGTCATAGGGGACTTGGTAACCACTCTCACTTTATGCAAAGGTCTGCCTCTGGCCTACAATCGTGATTTGCAGGAAGACAAGGAACCTGTATTTGACAGCGTAAAAACGATATTGGGGATGCTGGAAGTGTCAGCTGAGTTTGCTCAGAATATTTCTTTCAATCGGGAGAGAATACAAAAGGCTTTGCCTGCTGGTTATCTTGATGCCACAACACTTGCCGATTACCTCGTGAAGAAGGGAATACCTTTCAGAACAGGTCATGATATAGTTGGGAAGTCCGTTGCCTTGTGTGTCACAAAGAATTGCTGCCTCCAGGACTTGAGTCTGGATGAGCTAAGAAGCATAAGTCCAGTATTTGAAGAGGACGTTTACGAGTTTCTCGGCGCAGAGAATGCGATTAATAAATTTTGTTCATATGGATCGACAGGAGCAGCCTGTGTTGCCACTCAAATCGAATACTGGGTTAATAAACTCCAAATCAGTCTGTAG

Coding sequence (CDS)

ATGTATGACGTATGTGTCAACAAAATATCATATGACACTACACACTTAAGAGCTCCAACTCGCCGACGACGCGCCGCCAGTCGTCAGAATTTCCAGCCGCCGAAGATGGATTGCTTGGCCCTCCATTCCTCTTTACCCGCCGCATTTGCCTTCTCTAAGCCCTCCGTTTCAAGCTCTCAGCCGCCATTTCTCGCCGTCGCTCGTGGAGCCATGCCGGTGTCTGGGGTTCTCAAAGCATCTCGCACTGCCGATGTGGCGCCGTCCGTCGCCGACTCGCCGGAGAACCGAACCGACAAGGAGGTTAAGCTTTGGGGCGGTCGTTTCGAGGAGAGCGTCACCGATGCAGTTGAGCGATTCACCGAATCGATCTCCTACGATAAGCAGCTTTACAAGCACGATATCAGAGGAAGTCGAGCTCATGCTACTATGCTAGCTAAGCAGGGACTGATGAGTGTTGATGAAAGGGACAGTATTCTTGAAGGTTTGGATGAAATTGAGAGGCGCATAGAAAGTGGTGATTTTGTTTGGAGAACAGATAGGGAGGATGTTCATATGAACATTGAGGCTGCCTTGATTGATATGATTGGTGAGCCTGCAAAGAAGCTACATACCGCTCGAAGCCGCAACGATCAAGTTCTGACTGATTTTCGATTGTGGTGTCGGGATGCAATTGATAATATATTAGATGCTATTAAGTATCTTCAGGTTTCCATGGTAACTCTGGCTTTGAAGAATGAGGGAGTAATCGTTCCGGGCTATACACATTTACAACGAGCGCAGCCCGTTCTGCTTCAGCATCTGCTCTTAGCATTTGTTGAGCAGCTTGAAAGAGATGCTGGACGTTTGTCGGATTGCAGAGTTAGGCTAAATCTTTGCCCGCTAGGTGCATGTGCGCTAGCCGGCACAGGCCTTCCAATTGATCGTTTTATGACTGCTGAGGCTTTAGGGTTCACCGGTCCCTTGAGAAACAGCATTGATGCAGTTTCAGATCGAGATTTTGCCTTGGAGTTTCTTTCTGCCAATGCCATTACGGCTATTCATCTTTCAAGGCTTGGAGAAGAATGGGTGTTGTGGGCTTCTGAAGAATTCGGATTTATAACGCCAAATGATTCTGTTTCAACTGGAAGTAGCATAATGCCTCAGAAGAAAAATCCAGATCCAATGGAACTCGTTCGTGGAAAATCCGCTAGAGTCATAGGGGACTTGGTAACCACTCTCACTTTATGCAAAGGTCTGCCTCTGGCCTACAATCGTGATTTGCAGGAAGACAAGGAACCTGTATTTGACAGCGTAAAAACGATATTGGGGATGCTGGAAGTGTCAGCTGAGTTTGCTCAGAATATTTCTTTCAATCGGGAGAGAATACAAAAGGCTTTGCCTGCTGGTTATCTTGATGCCACAACACTTGCCGATTACCTCGTGAAGAAGGGAATACCTTTCAGAACAGGTCATGATATAGTTGGGAAGTCCGTTGCCTTGTGTGTCACAAAGAATTGCTGCCTCCAGGACTTGAGTCTGGATGAGCTAAGAAGCATAAGTCCAGTATTTGAAGAGGACGTTTACGAGTTTCTCGGCGCAGAGAATGCGATTAATAAATTTTGTTCATATGGATCGACAGGAGCAGCCTGTGTTGCCACTCAAATCGAATACTGGGTTAATAAACTCCAAATCAGTCTGTAG

Protein sequence

MYDVCVNKISYDTTHLRAPTRRRRAASRQNFQPPKMDCLALHSSLPAAFAFSKPSVSSSQPPFLAVARGAMPVSGVLKASRTADVAPSVADSPENRTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDERDSILEGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIVPGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERIQKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKNCCLQDLSLDELRSISPVFEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQISL
Homology
BLAST of Lag0037419 vs. NCBI nr
Match: XP_008466162.1 (PREDICTED: argininosuccinate lyase, chloroplastic [Cucumis melo] >KAA0038666.1 argininosuccinate lyase [Cucumis melo var. makuwa] >TYK31276.1 argininosuccinate lyase [Cucumis melo var. makuwa])

HSP 1 Score: 992.3 bits (2564), Expect = 1.7e-285
Identity = 500/523 (95.60%), Postives = 510/523 (97.51%), Query Frame = 0

Query: 36  MDCLALHSSLPAAFAFSKPSVSSSQPPFLAVARGAMPVSGVLKASRTADVAPSVADSPEN 95
           MDCL  + SLPAAFAFSKPSVS S+PPFLAVARGAMP+ GVL+AS TA+  PSV DSPEN
Sbjct: 1   MDCLTSY-SLPAAFAFSKPSVSLSKPPFLAVARGAMPMFGVLRASHTANAPPSVYDSPEN 60

Query: 96  RTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDER 155
           RTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDER
Sbjct: 61  RTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDER 120

Query: 156 DSILEGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD 215
           DSILEGLD+IER+IESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD
Sbjct: 121 DSILEGLDQIERQIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD 180

Query: 216 FRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIVPGYTHLQRAQPVLLQHLLLAFVEQL 275
           FRLWCRDAID ILDAIKYLQVSMVTLALKNEG+IVPGYTHLQRAQPVLLQHLLLAFVEQL
Sbjct: 181 FRLWCRDAIDAILDAIKYLQVSMVTLALKNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQL 240

Query: 276 ERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFAL 335
           ERDAGRLSDCRVRLN  PLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFAL
Sbjct: 241 ERDAGRLSDCRVRLNFSPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFAL 300

Query: 336 EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS 395
           EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS
Sbjct: 301 EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS 360

Query: 396 ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI 455
           ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI
Sbjct: 361 ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI 420

Query: 456 QKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKNCCLQDLSLDELRSISPV 515
           +KALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTK CCLQDLSLDELRSISPV
Sbjct: 421 EKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKKCCLQDLSLDELRSISPV 480

Query: 516 FEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQIS 559
           FEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQI+
Sbjct: 481 FEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQIN 522

BLAST of Lag0037419 vs. NCBI nr
Match: XP_038900244.1 (argininosuccinate lyase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 989.2 bits (2556), Expect = 1.5e-284
Identity = 495/524 (94.47%), Postives = 510/524 (97.33%), Query Frame = 0

Query: 36  MDCLALHSSLPAAFAFSKPSVSSSQPPFLAVARGAMPVSGVLKASRTADVAPSVADSPEN 95
           MDCL  + SLPAA AFSKPSVS+S+PPFLAV RGA+PV GVL+ASRTAD  PSV DSPEN
Sbjct: 1   MDCLTSY-SLPAALAFSKPSVSASKPPFLAVVRGAVPVFGVLRASRTADAPPSVEDSPEN 60

Query: 96  RTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDER 155
           RTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSV ER
Sbjct: 61  RTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVHER 120

Query: 156 DSILEGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD 215
           DSILEGLD+IER+IESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD
Sbjct: 121 DSILEGLDKIERQIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD 180

Query: 216 FRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIVPGYTHLQRAQPVLLQHLLLAFVEQL 275
           FRLWCRDAID ILDA+KYLQVSMVTLALKNEG+IVPGYTHLQRAQPVLLQHLLLAFVEQL
Sbjct: 181 FRLWCRDAIDTILDAMKYLQVSMVTLALKNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQL 240

Query: 276 ERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFAL 335
           ERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFT PLRNSIDAVSDRDFAL
Sbjct: 241 ERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTAPLRNSIDAVSDRDFAL 300

Query: 336 EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS 395
           EFLSANAI AIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS
Sbjct: 301 EFLSANAIAAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS 360

Query: 396 ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI 455
           ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI
Sbjct: 361 ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI 420

Query: 456 QKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKNCCLQDLSLDELRSISPV 515
           +KALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKNCCLQDLSLDELRSISPV
Sbjct: 421 EKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKNCCLQDLSLDELRSISPV 480

Query: 516 FEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQISL 560
           F+EDVYEFLGAENAINKFCSYGSTG ACVATQIEYW+NKL+I++
Sbjct: 481 FDEDVYEFLGAENAINKFCSYGSTGKACVATQIEYWINKLEINV 523

BLAST of Lag0037419 vs. NCBI nr
Match: XP_004136255.1 (argininosuccinate lyase, chloroplastic [Cucumis sativus] >KGN60260.1 hypothetical protein Csa_001984 [Cucumis sativus])

HSP 1 Score: 978.8 bits (2529), Expect = 2.0e-281
Identity = 492/523 (94.07%), Postives = 505/523 (96.56%), Query Frame = 0

Query: 36  MDCLALHSSLPAAFAFSKPSVSSSQPPFLAVARGAMPVSGVLKASRTADVAPSVADSPEN 95
           MDCL  + SLPAAF FS PSVS S+PPF AVARGAM + GVL+AS TA+  PSV DSPEN
Sbjct: 1   MDCLTSY-SLPAAFTFSNPSVSLSKPPFFAVARGAMSMVGVLRASHTANAPPSVDDSPEN 60

Query: 96  RTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDER 155
           RTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDER
Sbjct: 61  RTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDER 120

Query: 156 DSILEGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD 215
           DSILEGLD+IER+IESGDFVWRTDREDVHMNIEAALID+IGEPAKKLHTARSRNDQVLTD
Sbjct: 121 DSILEGLDQIERQIESGDFVWRTDREDVHMNIEAALIDIIGEPAKKLHTARSRNDQVLTD 180

Query: 216 FRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIVPGYTHLQRAQPVLLQHLLLAFVEQL 275
           FRLWCRDAID+ILDAIKYLQVSMVTLALKNEG+IVPGYTHLQRAQPVLLQHLLLAFVEQL
Sbjct: 181 FRLWCRDAIDSILDAIKYLQVSMVTLALKNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQL 240

Query: 276 ERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFAL 335
           ERDAGRLSDCRVRLN  PLGACALAGTGLPIDRFMTAEALGF GPLRNSIDAVSDRDFAL
Sbjct: 241 ERDAGRLSDCRVRLNFSPLGACALAGTGLPIDRFMTAEALGFAGPLRNSIDAVSDRDFAL 300

Query: 336 EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS 395
           EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS
Sbjct: 301 EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS 360

Query: 396 ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI 455
           ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTI+GMLEVSAEFAQNISFNRERI
Sbjct: 361 ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTIVGMLEVSAEFAQNISFNRERI 420

Query: 456 QKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKNCCLQDLSLDELRSISPV 515
            KALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTK CCLQDLSLDELRSISPV
Sbjct: 421 SKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKRCCLQDLSLDELRSISPV 480

Query: 516 FEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQIS 559
           FEEDVYEFLGAENAINKFCSYGSTGAACVATQI+YWVNKLQI+
Sbjct: 481 FEEDVYEFLGAENAINKFCSYGSTGAACVATQIDYWVNKLQIN 522

BLAST of Lag0037419 vs. NCBI nr
Match: XP_022976892.1 (argininosuccinate lyase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 973.8 bits (2516), Expect = 6.4e-280
Identity = 492/524 (93.89%), Postives = 505/524 (96.37%), Query Frame = 0

Query: 36  MDCLALHSSLPAAFAFSKPSVSSSQPPFLAVARGAMPVSGVLKASRTADVAPSVADSPEN 95
           MDCL  +  LPA  AFSK SVS+S+ PF AVARGA+PV GVL+ASRTAD   SV D PEN
Sbjct: 1   MDCLTSY-FLPATLAFSKSSVSASKLPFFAVARGALPVFGVLRASRTADAPLSVDDWPEN 60

Query: 96  RTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDER 155
           R+DKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHA+MLA QGLMSV ER
Sbjct: 61  RSDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLANQGLMSVAER 120

Query: 156 DSILEGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD 215
           DSI+EGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD
Sbjct: 121 DSIIEGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD 180

Query: 216 FRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIVPGYTHLQRAQPVLLQHLLLAFVEQL 275
           FRLWCRDAID ILDAI+YLQVSMVTLALKNEG+IVPGYTHLQRAQPVLLQHLLLAFVEQL
Sbjct: 181 FRLWCRDAIDAILDAIRYLQVSMVTLALKNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQL 240

Query: 276 ERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFAL 335
           ERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFT PLRNSIDAVSDRDFAL
Sbjct: 241 ERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTAPLRNSIDAVSDRDFAL 300

Query: 336 EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS 395
           EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS
Sbjct: 301 EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS 360

Query: 396 ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI 455
           ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI
Sbjct: 361 ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI 420

Query: 456 QKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKNCCLQDLSLDELRSISPV 515
           +KALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTK+CCLQDLSLDELRSISPV
Sbjct: 421 EKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKSCCLQDLSLDELRSISPV 480

Query: 516 FEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQISL 560
           FEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQI+L
Sbjct: 481 FEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQINL 523

BLAST of Lag0037419 vs. NCBI nr
Match: XP_023536382.1 (argininosuccinate lyase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 973.0 bits (2514), Expect = 1.1e-279
Identity = 491/524 (93.70%), Postives = 504/524 (96.18%), Query Frame = 0

Query: 36  MDCLALHSSLPAAFAFSKPSVSSSQPPFLAVARGAMPVSGVLKASRTADVAPSVADSPEN 95
           MDCL  +  LPA  AFSK SVS+S+ PF A ARGA+PV GVL+ASRTAD   SV DSPEN
Sbjct: 1   MDCLTSY-FLPATLAFSKSSVSASKLPFFAAARGALPVFGVLRASRTADAPLSVDDSPEN 60

Query: 96  RTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDER 155
           R DKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHA+MLA QGLMSV ER
Sbjct: 61  RDDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLASQGLMSVAER 120

Query: 156 DSILEGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD 215
           DSI+EGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD
Sbjct: 121 DSIIEGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD 180

Query: 216 FRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIVPGYTHLQRAQPVLLQHLLLAFVEQL 275
           FRLWCRDAID ILDAI+YLQVSMVTLALKNEG+IVPGYTHLQRAQPVLLQHLLLAFVEQL
Sbjct: 181 FRLWCRDAIDAILDAIRYLQVSMVTLALKNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQL 240

Query: 276 ERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFAL 335
           ERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFT PLRNSIDAVSDRDFAL
Sbjct: 241 ERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTAPLRNSIDAVSDRDFAL 300

Query: 336 EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS 395
           EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS
Sbjct: 301 EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS 360

Query: 396 ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI 455
           ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI
Sbjct: 361 ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI 420

Query: 456 QKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKNCCLQDLSLDELRSISPV 515
           +KALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTK+CCLQDLSLDELRSISPV
Sbjct: 421 EKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKSCCLQDLSLDELRSISPV 480

Query: 516 FEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQISL 560
           FEEDVYEFLGAENAINKFCSYGSTGAACVATQ+EYWVNKLQI+L
Sbjct: 481 FEEDVYEFLGAENAINKFCSYGSTGAACVATQMEYWVNKLQINL 523

BLAST of Lag0037419 vs. ExPASy Swiss-Prot
Match: Q9LEU8 (Argininosuccinate lyase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g10920 PE=2 SV=1)

HSP 1 Score: 747.3 bits (1928), Expect = 1.3e-214
Identity = 376/524 (71.76%), Postives = 435/524 (83.02%), Query Frame = 0

Query: 36  MDCLALHSSLPAAFAFSKPSVSSSQPPFLAVARGAMPVSGVLKASRTADVAPSVADSPEN 95
           M  + L  S    F+ S+ ++SSS             VS +   S++  + P  + S ++
Sbjct: 1   MGAIDLSFSQSLLFSSSRSNLSSST---------HRSVSFLPPGSKSRCLPPLRSMSHDD 60

Query: 96  RT-DKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDE 155
            T  KEVKLWGGRFEESVT+ VE+FTESIS+DK LYK DI GS+AHA+MLA QGL++  +
Sbjct: 61  DTASKEVKLWGGRFEESVTEKVEKFTESISFDKVLYKQDIMGSKAHASMLAHQGLITDSD 120

Query: 156 RDSILEGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT 215
           +DSIL GLD+IER+IE+  F WRTDREDVHMNIEAAL D+IGEPAKKLHTARSRNDQV T
Sbjct: 121 KDSILRGLDDIERQIEANKFEWRTDREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVAT 180

Query: 216 DFRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIVPGYTHLQRAQPVLLQHLLLAFVEQ 275
           DFRLWCRDAID I+  I+ LQ ++V LALKNE +IVPGYTHLQRAQPVLL H+LL FVEQ
Sbjct: 181 DFRLWCRDAIDTIIVKIRNLQRALVELALKNEALIVPGYTHLQRAQPVLLPHVLLTFVEQ 240

Query: 276 LERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFA 335
           LERDAGR  DCR RLN  PLGACALAGTGLPIDRFMTA ALGFT P+RNSIDAVSDRDF 
Sbjct: 241 LERDAGRYVDCRARLNFSPLGACALAGTGLPIDRFMTANALGFTEPMRNSIDAVSDRDFV 300

Query: 336 LEFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGK 395
           LEFL  NA T IHLSRLGEEWVLWASEEFGF+TP+DSVSTGSSIMPQKKNPDPMELVRGK
Sbjct: 301 LEFLYTNANTGIHLSRLGEEWVLWASEEFGFMTPSDSVSTGSSIMPQKKNPDPMELVRGK 360

Query: 396 SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRER 455
           SARVIGDLVT LTLCKGLPLAYNRD QEDKEP+FDS KTI+GM++VSAEFAQN++FN +R
Sbjct: 361 SARVIGDLVTVLTLCKGLPLAYNRDFQEDKEPMFDSTKTIMGMIDVSAEFAQNVTFNEDR 420

Query: 456 IQKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKNCCLQDLSLDELRSISP 515
           I+K+LPAG+LDATTLADYLVKKG+PFR+ HDIVGK V +CV+K C LQ+LSL+E++ +SP
Sbjct: 421 IKKSLPAGHLDATTLADYLVKKGMPFRSSHDIVGKLVGVCVSKGCELQNLSLEEMKKLSP 480

Query: 516 VFEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQIS 559
           VFEEDV+ FLG EN++NKF SYGSTG+ CVA Q+ YWVNKL I+
Sbjct: 481 VFEEDVFGFLGVENSVNKFSSYGSTGSNCVAEQLGYWVNKLNIT 515

BLAST of Lag0037419 vs. ExPASy Swiss-Prot
Match: Q6AR60 (Argininosuccinate lyase OS=Desulfotalea psychrophila (strain LSv54 / DSM 12343) OX=177439 GN=argH PE=3 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 2.2e-134
Identity = 232/442 (52.49%), Postives = 307/442 (69.46%), Query Frame = 0

Query: 99  KEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDERDSI 158
           K  KLWGGRF E++  +VE+F+ESISYD +LYK+DI GS+AHATML+ QG++S +E + I
Sbjct: 5   KSKKLWGGRFSEAMAASVEKFSESISYDVRLYKYDIAGSKAHATMLSSQGIISPEELEQI 64

Query: 159 LEGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTDFRL 218
           + GL  IE  IE+G F ++T+ EDVHMNIE AL+D IG    +LH ARSRNDQ+  DF++
Sbjct: 65  IAGLSSIEADIEAGVFEFKTEYEDVHMNIEQALVDRIGAAGSRLHAARSRNDQIALDFKM 124

Query: 219 WCRDAIDNILDAIKYLQVSMVTLALKNEGVIVPGYTHLQRAQPVLLQHLLLAFVEQLERD 278
           + RD  D++++ +     +   +  K  G I+PGYTH QRAQPVL+ H +LA+ E   RD
Sbjct: 125 YLRDQCDHLVELLDGACRAFTVVGRKYLGDIMPGYTHTQRAQPVLITHHMLAYYEMFRRD 184

Query: 279 AGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFALEFL 338
             R+ DCR RLNL PLG  A+AGTGLPI+R   A+ALGF G   NS+D  +DRD+A+E  
Sbjct: 185 RDRILDCRKRLNLSPLGCAAMAGTGLPINREQVAKALGFAGVTANSMDTSADRDYAIELT 244

Query: 339 SANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKSARV 398
           S   +  +HLSRL EE V W++ E+ F+  +DS  TGSSIMPQKKNPD  EL+RGKS RV
Sbjct: 245 SCLTMIQLHLSRLAEELVTWSTSEYKFVDISDSFCTGSSIMPQKKNPDIAELIRGKSGRV 304

Query: 399 IGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERIQKA 458
           +G L++ +T+ KGLPL YNRD QEDKEPVFD++ T+   L ++AE   ++ FN  R  +A
Sbjct: 305 VGSLISLITMMKGLPLTYNRDQQEDKEPVFDAIDTVSASLSITAEMMAHMKFNTARCAEA 364

Query: 459 LPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKNCCLQDLSLDELRSISPVFEE 518
              G++ AT LADYLV K +PFR  H IVG +VA C+ K C L DL+L E++  SPV E 
Sbjct: 365 TETGFITATDLADYLVMKDVPFRQAHHIVGSAVAACIAKGCELPDLTLTEMQEFSPVIES 424

Query: 519 DVYEFLGAENAINKFCSYGSTG 541
           DV+  L AE ++N   S G TG
Sbjct: 425 DVFAVLTAEGSVNSRVSTGGTG 446

BLAST of Lag0037419 vs. ExPASy Swiss-Prot
Match: B8GSV2 (Argininosuccinate lyase OS=Thioalkalivibrio sulfidiphilus (strain HL-EbGR7) OX=396588 GN=argH PE=3 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 4.6e-132
Identity = 239/447 (53.47%), Postives = 306/447 (68.46%), Query Frame = 0

Query: 102 KLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDERDSILEG 161
           KLWGGRF E     VE FT S+S+D++LY+HDI+GS+AHA MLA+ G+++ +E D I +G
Sbjct: 9   KLWGGRFSEPTDAFVEAFTASVSFDQRLYRHDIQGSKAHARMLARVGVLTEEECDKIHDG 68

Query: 162 LDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTDFRLWCR 221
           LD I   IE GDF W  + EDVHMN+EA LI+ IG+  KKLHT RSRNDQV TD RL+ R
Sbjct: 69  LDAILADIERGDFAWSVELEDVHMNVEARLIERIGDVGKKLHTGRSRNDQVATDIRLYLR 128

Query: 222 DAIDNILDAIKYLQVSMVTLALKNEGVIVPGYTHLQRAQPVLLQHLLLAFVEQLERDAGR 281
           +AID ++  I+ LQ  +V LA +    I+PG+THLQ AQPV   H ++A+ E LERD GR
Sbjct: 129 EAIDALMQEIRRLQGGLVDLAEREAQTIMPGFTHLQTAQPVTFGHHMMAWYEMLERDHGR 188

Query: 282 LSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFALEFLSAN 341
           L+DCR R+N+ PLGA ALAGT  P+DR  TAE LGF     NS+DAVSDRDFA+EF +A 
Sbjct: 189 LADCRKRVNVLPLGAAALAGTPYPLDRHYTAELLGFDAVTDNSLDAVSDRDFAIEFCAAG 248

Query: 342 AITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKSARVIGD 401
           A+   HLSR  EE +LWAS +FGF+   D   TGSSIMPQKKNPD  ELVRGK+ R  G+
Sbjct: 249 ALIMTHLSRFSEELILWASAQFGFVDLPDRFCTGSSIMPQKKNPDVPELVRGKTGRANGN 308

Query: 402 LVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERIQKALPA 461
           L+  LTL KG PLAYN+D QEDKEP+FD+V T+ G L   A+   +I    E ++ A   
Sbjct: 309 LIALLTLMKGQPLAYNKDNQEDKEPLFDTVDTLAGSLRAFADMVPHIQVRAEVMRAAASR 368

Query: 462 GYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKNCCLQDLSLDELRSISPVFEEDVY 521
           G+  AT LADYLV++G+PFR  H++VG++V   V     L ++SLDELR  S   E+DV+
Sbjct: 369 GFATATDLADYLVRRGMPFRDAHEVVGRAVRHGVETGKDLAEMSLDELRKFSKTIEQDVF 428

Query: 522 EFLGAENAINKFCSYGSTGAACVATQI 549
           E L  E ++     +G T  A V  +I
Sbjct: 429 EVLTLEGSVAARNVHGGTAPAQVRARI 455

BLAST of Lag0037419 vs. ExPASy Swiss-Prot
Match: Q3JDS2 (Argininosuccinate lyase OS=Nitrosococcus oceani (strain ATCC 19707 / BCRC 17464 / NCIMB 11848 / C-107) OX=323261 GN=argH PE=3 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 2.5e-130
Identity = 245/454 (53.96%), Postives = 304/454 (66.96%), Query Frame = 0

Query: 102 KLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDERDSILEG 161
           K W GRF E     VE FT SI +D +LY+ DI GS AHA MLAK G++S +E D+I++G
Sbjct: 10  KPWSGRFHEPTDTFVEEFTASIGFDHRLYRQDIHGSIAHARMLAKVGVLSREECDAIIQG 69

Query: 162 LDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTDFRLWCR 221
           L+ I + I  G F W    EDVHMNIEAAL + IG   KKLHT RSRNDQ+ TD RL+ R
Sbjct: 70  LETIAQDISRGQFSWSVALEDVHMNIEAALTERIGPVGKKLHTGRSRNDQIATDIRLYLR 129

Query: 222 DAIDNILDAIKYLQVSMVTLALKNEGVIVPGYTHLQRAQPVLLQHLLLAFVEQLERDAGR 281
           DAID I   ++ LQ  ++ +A +    I+PG+THLQ AQPV   H L+A+ E L RD GR
Sbjct: 130 DAIDLITAQLQRLQEGLLAIAEREAATIMPGFTHLQTAQPVTFGHHLMAWFEMLLRDTGR 189

Query: 282 LSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFALEFLSAN 341
           L+DCR R+N+ PLGA ALAGT  PIDR  TAE LGF G   NS+DAVSDRDFA+EF +A 
Sbjct: 190 LADCRHRVNVLPLGAAALAGTTFPIDRAYTAELLGFDGIAENSLDAVSDRDFAIEFTAAG 249

Query: 342 AITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKSARVIGD 401
           A+   HLSR  EE VLW+S +F FIT  D   TGSSIMPQKKNPD  ELVRGK+ RV G 
Sbjct: 250 ALLMTHLSRFAEELVLWSSAQFDFITLPDRFCTGSSIMPQKKNPDVPELVRGKTGRVNGH 309

Query: 402 LVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERIQKALPA 461
           LV+ LTL KG PLAYN+D QEDKEP+FD+V T+LG L   A+    I+ N +++++A   
Sbjct: 310 LVSLLTLMKGQPLAYNKDNQEDKEPLFDTVDTLLGCLRAFADMIPAITTNPDKMREAATK 369

Query: 462 GYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKNCCLQDLSLDELRSISPVFEEDVY 521
           GY  AT LADYLV+KG+ FR  H+IVGK+VAL + ++  L  L L  L+  SPV EEDV+
Sbjct: 370 GYATATDLADYLVRKGVSFRDAHEIVGKAVALAIEQSKDLAALKLATLQDFSPVIEEDVF 429

Query: 522 EFLGAENAINKFCSYGSTGAACVATQIEYWVNKL 556
           + L  E ++      G T  A V   I+    KL
Sbjct: 430 DVLTLEGSVAARNHLGGTAPAQVRAAIQRGREKL 463

BLAST of Lag0037419 vs. ExPASy Swiss-Prot
Match: Q2SQ67 (Argininosuccinate lyase OS=Hahella chejuensis (strain KCTC 2396) OX=349521 GN=argH PE=3 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 1.3e-129
Identity = 237/444 (53.38%), Postives = 298/444 (67.12%), Query Frame = 0

Query: 96  RTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDER 155
           + D   KLWGGRF E+    VERFT S+ +D+++Y HDIRGS AHATMLAK G+++ +ER
Sbjct: 3   KQDTTDKLWGGRFTEATDAFVERFTASVDFDRRMYHHDIRGSIAHATMLAKVGVLTEEER 62

Query: 156 DSILEGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD 215
           D I+ GL EIE  IE+G F W    EDVHMNIEA L   IG   KKLHT RSRNDQV TD
Sbjct: 63  DQIINGLREIEAEIEAGAFEWSVKLEDVHMNIEARLTQKIGITGKKLHTGRSRNDQVATD 122

Query: 216 FRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIVPGYTHLQRAQPVLLQHLLLAFVEQL 275
            RL+ RD +D I   +  L  ++  LA +    I+PG+THLQ AQPV   H +LA+ E L
Sbjct: 123 IRLYLRDEVDVIAAELLRLIAALADLAEREADAIMPGFTHLQTAQPVTFGHHMLAWAEML 182

Query: 276 ERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFAL 335
           +RD  RL+DCR R N+ PLGA ALAGT   IDR  T+E LGF GP  NS+DAVSDRDFA+
Sbjct: 183 KRDFSRLADCRERFNVSPLGAAALAGTTYSIDRQYTSELLGFNGPAENSLDAVSDRDFAI 242

Query: 336 EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS 395
           EF S  ++  +HLSR  EE VLW S +F FI   D   TGSSIMPQKKNPD  EL+RGKS
Sbjct: 243 EFCSFASLLMMHLSRFSEELVLWTSAQFNFINLPDRFCTGSSIMPQKKNPDVPELIRGKS 302

Query: 396 ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI 455
            RV G L++ L L K  PLAYN+D QEDKEP+FD++ T+ G L+   +    +S NR+ +
Sbjct: 303 GRVSGHLISLLMLMKSQPLAYNKDNQEDKEPLFDAIDTVKGCLKAYGDMMPAVSVNRDAM 362

Query: 456 QKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKNCCLQDLSLDELRSISPV 515
            ++   G+  AT LADYLV+KG+PFR  H++VGK+VAL V+    L ++SL+ELR  S  
Sbjct: 363 AESARRGFSTATDLADYLVRKGLPFRDAHEVVGKAVALGVSSGRDLSEMSLEELREFSAD 422

Query: 516 FEEDVYEFLGAENAINKFCSYGST 540
            E DV+E L  E ++N     G T
Sbjct: 423 IEVDVFEVLTLEGSVNARNHIGGT 446

BLAST of Lag0037419 vs. ExPASy TrEMBL
Match: A0A5A7T8Y2 (Argininosuccinate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005690 PE=3 SV=1)

HSP 1 Score: 992.3 bits (2564), Expect = 8.4e-286
Identity = 500/523 (95.60%), Postives = 510/523 (97.51%), Query Frame = 0

Query: 36  MDCLALHSSLPAAFAFSKPSVSSSQPPFLAVARGAMPVSGVLKASRTADVAPSVADSPEN 95
           MDCL  + SLPAAFAFSKPSVS S+PPFLAVARGAMP+ GVL+AS TA+  PSV DSPEN
Sbjct: 1   MDCLTSY-SLPAAFAFSKPSVSLSKPPFLAVARGAMPMFGVLRASHTANAPPSVYDSPEN 60

Query: 96  RTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDER 155
           RTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDER
Sbjct: 61  RTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDER 120

Query: 156 DSILEGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD 215
           DSILEGLD+IER+IESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD
Sbjct: 121 DSILEGLDQIERQIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD 180

Query: 216 FRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIVPGYTHLQRAQPVLLQHLLLAFVEQL 275
           FRLWCRDAID ILDAIKYLQVSMVTLALKNEG+IVPGYTHLQRAQPVLLQHLLLAFVEQL
Sbjct: 181 FRLWCRDAIDAILDAIKYLQVSMVTLALKNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQL 240

Query: 276 ERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFAL 335
           ERDAGRLSDCRVRLN  PLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFAL
Sbjct: 241 ERDAGRLSDCRVRLNFSPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFAL 300

Query: 336 EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS 395
           EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS
Sbjct: 301 EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS 360

Query: 396 ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI 455
           ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI
Sbjct: 361 ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI 420

Query: 456 QKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKNCCLQDLSLDELRSISPV 515
           +KALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTK CCLQDLSLDELRSISPV
Sbjct: 421 EKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKKCCLQDLSLDELRSISPV 480

Query: 516 FEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQIS 559
           FEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQI+
Sbjct: 481 FEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQIN 522

BLAST of Lag0037419 vs. ExPASy TrEMBL
Match: A0A1S3CQK7 (argininosuccinate lyase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503657 PE=3 SV=1)

HSP 1 Score: 992.3 bits (2564), Expect = 8.4e-286
Identity = 500/523 (95.60%), Postives = 510/523 (97.51%), Query Frame = 0

Query: 36  MDCLALHSSLPAAFAFSKPSVSSSQPPFLAVARGAMPVSGVLKASRTADVAPSVADSPEN 95
           MDCL  + SLPAAFAFSKPSVS S+PPFLAVARGAMP+ GVL+AS TA+  PSV DSPEN
Sbjct: 1   MDCLTSY-SLPAAFAFSKPSVSLSKPPFLAVARGAMPMFGVLRASHTANAPPSVYDSPEN 60

Query: 96  RTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDER 155
           RTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDER
Sbjct: 61  RTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDER 120

Query: 156 DSILEGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD 215
           DSILEGLD+IER+IESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD
Sbjct: 121 DSILEGLDQIERQIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD 180

Query: 216 FRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIVPGYTHLQRAQPVLLQHLLLAFVEQL 275
           FRLWCRDAID ILDAIKYLQVSMVTLALKNEG+IVPGYTHLQRAQPVLLQHLLLAFVEQL
Sbjct: 181 FRLWCRDAIDAILDAIKYLQVSMVTLALKNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQL 240

Query: 276 ERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFAL 335
           ERDAGRLSDCRVRLN  PLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFAL
Sbjct: 241 ERDAGRLSDCRVRLNFSPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFAL 300

Query: 336 EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS 395
           EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS
Sbjct: 301 EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS 360

Query: 396 ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI 455
           ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI
Sbjct: 361 ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI 420

Query: 456 QKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKNCCLQDLSLDELRSISPV 515
           +KALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTK CCLQDLSLDELRSISPV
Sbjct: 421 EKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKKCCLQDLSLDELRSISPV 480

Query: 516 FEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQIS 559
           FEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQI+
Sbjct: 481 FEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQIN 522

BLAST of Lag0037419 vs. ExPASy TrEMBL
Match: A0A0A0LE21 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G891650 PE=3 SV=1)

HSP 1 Score: 978.8 bits (2529), Expect = 9.6e-282
Identity = 492/523 (94.07%), Postives = 505/523 (96.56%), Query Frame = 0

Query: 36  MDCLALHSSLPAAFAFSKPSVSSSQPPFLAVARGAMPVSGVLKASRTADVAPSVADSPEN 95
           MDCL  + SLPAAF FS PSVS S+PPF AVARGAM + GVL+AS TA+  PSV DSPEN
Sbjct: 1   MDCLTSY-SLPAAFTFSNPSVSLSKPPFFAVARGAMSMVGVLRASHTANAPPSVDDSPEN 60

Query: 96  RTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDER 155
           RTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDER
Sbjct: 61  RTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDER 120

Query: 156 DSILEGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD 215
           DSILEGLD+IER+IESGDFVWRTDREDVHMNIEAALID+IGEPAKKLHTARSRNDQVLTD
Sbjct: 121 DSILEGLDQIERQIESGDFVWRTDREDVHMNIEAALIDIIGEPAKKLHTARSRNDQVLTD 180

Query: 216 FRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIVPGYTHLQRAQPVLLQHLLLAFVEQL 275
           FRLWCRDAID+ILDAIKYLQVSMVTLALKNEG+IVPGYTHLQRAQPVLLQHLLLAFVEQL
Sbjct: 181 FRLWCRDAIDSILDAIKYLQVSMVTLALKNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQL 240

Query: 276 ERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFAL 335
           ERDAGRLSDCRVRLN  PLGACALAGTGLPIDRFMTAEALGF GPLRNSIDAVSDRDFAL
Sbjct: 241 ERDAGRLSDCRVRLNFSPLGACALAGTGLPIDRFMTAEALGFAGPLRNSIDAVSDRDFAL 300

Query: 336 EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS 395
           EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS
Sbjct: 301 EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS 360

Query: 396 ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI 455
           ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTI+GMLEVSAEFAQNISFNRERI
Sbjct: 361 ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTIVGMLEVSAEFAQNISFNRERI 420

Query: 456 QKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKNCCLQDLSLDELRSISPV 515
            KALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTK CCLQDLSLDELRSISPV
Sbjct: 421 SKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKRCCLQDLSLDELRSISPV 480

Query: 516 FEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQIS 559
           FEEDVYEFLGAENAINKFCSYGSTGAACVATQI+YWVNKLQI+
Sbjct: 481 FEEDVYEFLGAENAINKFCSYGSTGAACVATQIDYWVNKLQIN 522

BLAST of Lag0037419 vs. ExPASy TrEMBL
Match: A0A6J1IGY4 (argininosuccinate lyase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111477131 PE=3 SV=1)

HSP 1 Score: 973.8 bits (2516), Expect = 3.1e-280
Identity = 492/524 (93.89%), Postives = 505/524 (96.37%), Query Frame = 0

Query: 36  MDCLALHSSLPAAFAFSKPSVSSSQPPFLAVARGAMPVSGVLKASRTADVAPSVADSPEN 95
           MDCL  +  LPA  AFSK SVS+S+ PF AVARGA+PV GVL+ASRTAD   SV D PEN
Sbjct: 1   MDCLTSY-FLPATLAFSKSSVSASKLPFFAVARGALPVFGVLRASRTADAPLSVDDWPEN 60

Query: 96  RTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDER 155
           R+DKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHA+MLA QGLMSV ER
Sbjct: 61  RSDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLANQGLMSVAER 120

Query: 156 DSILEGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD 215
           DSI+EGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD
Sbjct: 121 DSIIEGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD 180

Query: 216 FRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIVPGYTHLQRAQPVLLQHLLLAFVEQL 275
           FRLWCRDAID ILDAI+YLQVSMVTLALKNEG+IVPGYTHLQRAQPVLLQHLLLAFVEQL
Sbjct: 181 FRLWCRDAIDAILDAIRYLQVSMVTLALKNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQL 240

Query: 276 ERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFAL 335
           ERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFT PLRNSIDAVSDRDFAL
Sbjct: 241 ERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTAPLRNSIDAVSDRDFAL 300

Query: 336 EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS 395
           EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS
Sbjct: 301 EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS 360

Query: 396 ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI 455
           ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI
Sbjct: 361 ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI 420

Query: 456 QKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKNCCLQDLSLDELRSISPV 515
           +KALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTK+CCLQDLSLDELRSISPV
Sbjct: 421 EKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKSCCLQDLSLDELRSISPV 480

Query: 516 FEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQISL 560
           FEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQI+L
Sbjct: 481 FEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQINL 523

BLAST of Lag0037419 vs. ExPASy TrEMBL
Match: A0A6J1FFD9 (argininosuccinate lyase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111443572 PE=3 SV=1)

HSP 1 Score: 971.5 bits (2510), Expect = 1.5e-279
Identity = 489/524 (93.32%), Postives = 505/524 (96.37%), Query Frame = 0

Query: 36  MDCLALHSSLPAAFAFSKPSVSSSQPPFLAVARGAMPVSGVLKASRTADVAPSVADSPEN 95
           MDCL  +   PA  AFSK SVS+S+ PF AVARGA+PV GVL+ASRTAD   SV DSPEN
Sbjct: 1   MDCLTSY-FFPATLAFSKSSVSASKLPFFAVARGALPVFGVLRASRTADAPLSVDDSPEN 60

Query: 96  RTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDER 155
           R+DKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHA+MLA QGL+SV ER
Sbjct: 61  RSDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLANQGLISVAER 120

Query: 156 DSILEGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD 215
           DSI+EGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD
Sbjct: 121 DSIIEGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTD 180

Query: 216 FRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIVPGYTHLQRAQPVLLQHLLLAFVEQL 275
           FRLWCRDAID ILDAI+YLQVSMVTLALKNEG+IVPGYTHLQRAQPVLLQHLLLAFVEQL
Sbjct: 181 FRLWCRDAIDAILDAIRYLQVSMVTLALKNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQL 240

Query: 276 ERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFAL 335
           ERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFT PLRNSIDAVSDRDFAL
Sbjct: 241 ERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTAPLRNSIDAVSDRDFAL 300

Query: 336 EFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGKS 395
           EFLSANAITAIHLSRLGEEWVLWASEEFGF+TPNDSVSTGSSIMPQKKNPDPMELVRGKS
Sbjct: 301 EFLSANAITAIHLSRLGEEWVLWASEEFGFVTPNDSVSTGSSIMPQKKNPDPMELVRGKS 360

Query: 396 ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI 455
           ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI
Sbjct: 361 ARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRERI 420

Query: 456 QKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKNCCLQDLSLDELRSISPV 515
           +KALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTK+CCLQDLSLDELRSISPV
Sbjct: 421 EKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKSCCLQDLSLDELRSISPV 480

Query: 516 FEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQISL 560
           FEEDVYEFLGAENAINKFCSYGSTGAACVATQI+YWVNKLQI+L
Sbjct: 481 FEEDVYEFLGAENAINKFCSYGSTGAACVATQIKYWVNKLQINL 523

BLAST of Lag0037419 vs. TAIR 10
Match: AT5G10920.1 (L-Aspartase-like family protein )

HSP 1 Score: 747.3 bits (1928), Expect = 9.1e-216
Identity = 376/524 (71.76%), Postives = 435/524 (83.02%), Query Frame = 0

Query: 36  MDCLALHSSLPAAFAFSKPSVSSSQPPFLAVARGAMPVSGVLKASRTADVAPSVADSPEN 95
           M  + L  S    F+ S+ ++SSS             VS +   S++  + P  + S ++
Sbjct: 1   MGAIDLSFSQSLLFSSSRSNLSSST---------HRSVSFLPPGSKSRCLPPLRSMSHDD 60

Query: 96  RT-DKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDE 155
            T  KEVKLWGGRFEESVT+ VE+FTESIS+DK LYK DI GS+AHA+MLA QGL++  +
Sbjct: 61  DTASKEVKLWGGRFEESVTEKVEKFTESISFDKVLYKQDIMGSKAHASMLAHQGLITDSD 120

Query: 156 RDSILEGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT 215
           +DSIL GLD+IER+IE+  F WRTDREDVHMNIEAAL D+IGEPAKKLHTARSRNDQV T
Sbjct: 121 KDSILRGLDDIERQIEANKFEWRTDREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVAT 180

Query: 216 DFRLWCRDAIDNILDAIKYLQVSMVTLALKNEGVIVPGYTHLQRAQPVLLQHLLLAFVEQ 275
           DFRLWCRDAID I+  I+ LQ ++V LALKNE +IVPGYTHLQRAQPVLL H+LL FVEQ
Sbjct: 181 DFRLWCRDAIDTIIVKIRNLQRALVELALKNEALIVPGYTHLQRAQPVLLPHVLLTFVEQ 240

Query: 276 LERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFA 335
           LERDAGR  DCR RLN  PLGACALAGTGLPIDRFMTA ALGFT P+RNSIDAVSDRDF 
Sbjct: 241 LERDAGRYVDCRARLNFSPLGACALAGTGLPIDRFMTANALGFTEPMRNSIDAVSDRDFV 300

Query: 336 LEFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGK 395
           LEFL  NA T IHLSRLGEEWVLWASEEFGF+TP+DSVSTGSSIMPQKKNPDPMELVRGK
Sbjct: 301 LEFLYTNANTGIHLSRLGEEWVLWASEEFGFMTPSDSVSTGSSIMPQKKNPDPMELVRGK 360

Query: 396 SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRER 455
           SARVIGDLVT LTLCKGLPLAYNRD QEDKEP+FDS KTI+GM++VSAEFAQN++FN +R
Sbjct: 361 SARVIGDLVTVLTLCKGLPLAYNRDFQEDKEPMFDSTKTIMGMIDVSAEFAQNVTFNEDR 420

Query: 456 IQKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKNCCLQDLSLDELRSISP 515
           I+K+LPAG+LDATTLADYLVKKG+PFR+ HDIVGK V +CV+K C LQ+LSL+E++ +SP
Sbjct: 421 IKKSLPAGHLDATTLADYLVKKGMPFRSSHDIVGKLVGVCVSKGCELQNLSLEEMKKLSP 480

Query: 516 VFEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQIS 559
           VFEEDV+ FLG EN++NKF SYGSTG+ CVA Q+ YWVNKL I+
Sbjct: 481 VFEEDVFGFLGVENSVNKFSSYGSTGSNCVAEQLGYWVNKLNIT 515

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008466162.11.7e-28595.60PREDICTED: argininosuccinate lyase, chloroplastic [Cucumis melo] >KAA0038666.1 a... [more]
XP_038900244.11.5e-28494.47argininosuccinate lyase, chloroplastic-like [Benincasa hispida][more]
XP_004136255.12.0e-28194.07argininosuccinate lyase, chloroplastic [Cucumis sativus] >KGN60260.1 hypothetica... [more]
XP_022976892.16.4e-28093.89argininosuccinate lyase, chloroplastic-like [Cucurbita maxima][more]
XP_023536382.11.1e-27993.70argininosuccinate lyase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9LEU81.3e-21471.76Argininosuccinate lyase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g10... [more]
Q6AR602.2e-13452.49Argininosuccinate lyase OS=Desulfotalea psychrophila (strain LSv54 / DSM 12343) ... [more]
B8GSV24.6e-13253.47Argininosuccinate lyase OS=Thioalkalivibrio sulfidiphilus (strain HL-EbGR7) OX=3... [more]
Q3JDS22.5e-13053.96Argininosuccinate lyase OS=Nitrosococcus oceani (strain ATCC 19707 / BCRC 17464 ... [more]
Q2SQ671.3e-12953.38Argininosuccinate lyase OS=Hahella chejuensis (strain KCTC 2396) OX=349521 GN=ar... [more]
Match NameE-valueIdentityDescription
A0A5A7T8Y28.4e-28695.60Argininosuccinate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3CQK78.4e-28695.60argininosuccinate lyase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503657 P... [more]
A0A0A0LE219.6e-28294.07Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G891650 PE=3 SV=1[more]
A0A6J1IGY43.1e-28093.89argininosuccinate lyase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1FFD91.5e-27993.32argininosuccinate lyase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT5G10920.19.1e-21671.76L-Aspartase-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000362Fumarate lyase familyPRINTSPR00149FUMRATELYASEcoord: 375..391
score: 64.09
coord: 247..265
score: 42.48
coord: 331..358
score: 27.87
coord: 202..220
score: 36.91
NoneNo IPR availablePRINTSPR00145ARGSUCLYASEcoord: 242..262
score: 58.77
coord: 375..391
score: 81.13
coord: 331..355
score: 52.07
coord: 410..429
score: 76.96
coord: 201..223
score: 68.79
coord: 293..309
score: 67.02
NoneNo IPR availableGENE3D1.20.200.10Fumarase/aspartase (Central domain)coord: 219..548
e-value: 8.6E-184
score: 613.3
NoneNo IPR availableGENE3D1.10.40.30coord: 460..532
e-value: 8.6E-184
score: 613.3
NoneNo IPR availablePANTHERPTHR43814:SF4ARGININOSUCCINATE LYASEcoord: 113..558
IPR029419Argininosuccinate lyase, C-terminalPFAMPF14698ASL_C2coord: 463..530
e-value: 3.4E-22
score: 78.9
IPR022761Fumarate lyase, N-terminalPFAMPF00206Lyase_1coord: 106..400
e-value: 2.0E-69
score: 234.4
IPR024083Fumarase/histidase, N-terminalGENE3D1.10.275.10coord: 122..329
e-value: 8.6E-184
score: 613.3
IPR009049Argininosuccinate lyaseTIGRFAMTIGR00838TIGR00838coord: 103..551
e-value: 5.2E-173
score: 574.2
IPR009049Argininosuccinate lyasePANTHERPTHR43814ARGININOSUCCINATE LYASEcoord: 113..558
IPR009049Argininosuccinate lyaseHAMAPMF_00006Arg_succ_lyasecoord: 102..553
score: 40.202625
IPR009049Argininosuccinate lyaseCDDcd01359Argininosuccinate_lyasecoord: 122..555
e-value: 0.0
score: 662.32
IPR020557Fumarate lyase, conserved sitePROSITEPS00163FUMARATE_LYASEScoord: 375..384
IPR008948L-Aspartase-likeSUPERFAMILY48557L-aspartase-likecoord: 103..555

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0037419.1Lag0037419.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042450 arginine biosynthetic process via ornithine
cellular_component GO:0005829 cytosol
molecular_function GO:0004056 argininosuccinate lyase activity
molecular_function GO:0003824 catalytic activity