Lag0037412 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0037412
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPectinesterase
Locationchr2: 5992757 .. 5997728 (-)
RNA-Seq ExpressionLag0037412
SyntenyLag0037412
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTTCTCCTTGCCAGTCCCCAGATCGATGGTGTGTGATTTGCTCCAAAAGTTTTCGGTTTCTGCGTCCTAACGACGACAGATCGAAAGCTCAAGAAACCACCGCACCACCGATCAGCGCAACATTTCGACGACGGGCAGAGGGAGCAATGCGTTTCGGTACGATCCCTTTTCCCCTTGTTTGTTTTCGGGTGGGATCTGTGGAGCTGTGTGGAATAAATGGAAGCAGAGAAAGAGAGAGAGAGATAACAGAAGAATTAGTAAGGTTTAACTTATTTGGCGGAAAACAGTTTGGGTTTGGCGCAGGAAGTTGAGGATTCATGGCTAATTTTGCTTCTTCTCTCCTTTGCATGGATCAAATTTGCTCCGATCAGATCGCTGGCTGTTGGTTATCACACAACAATCAAGGTTTCTGTATGGTACTCAGAGGTTAGTTATCTCTCTCTCTCTCTCTCTATAATACATTATTAAAAAGAATATATAGATTATTTCATATTTGATTTTAGGTTAAATTATGAGCGTATAGTTTCCAAACTTTGAGTGATTCCTTAATTTTAAAATCTATCATCTCTCTCTTTTGTCTCAAATAGGTTAATATATTCTTTATCTATTCAACATTTTTAAAAATTTACAAACTTATTGAACACGAAATTAAAAATTTAGGGTCTTATTATACATAAAATTCAATTTTATATCTAAAAGATCAATTAATTTTAAAAAATTTTAAATATGTGAAGAAGATATTAGGCACAAAATTGAAAGTGTAGTGACTTAGCTTATTAAATATTTTTAATGTTCATGGATTTATTAGACACAATTAAAAGTCTATGAACATATTGGACACTTTTTAAAGTTTAAAAATCATTTAGACACAAGTCTCAAAATTTAGAGACTAAATTTGTAATTTAAATTTAATTTTAATGAAAATCATAAATTTTAATGACTAATTATTGATTAAAATTGAATAAACTGTTTTTTCAAATAGATTTTTGGGGGCCTTTGGGCCATAGCCCTCTTTAATCCCATTATGGTCCGAACTAGTAACTCACATTGCTGGTAGCTTCAAAATGATAAAGTCTTCTCTATCCATGTCATTTTTATTTTTATTATTATTATTTTTTTAGTAAATCAATATTTGTGAGGGAGGAATTCGAATTTCTAACCTATAGATCGATGGTACGTGTTAATTACCACTGAGCTAAGCTTTGGTCTCTTTCATGTTAATATTTATATGTAAAAAATGTGTCAAGTGTATCTTTGTTTAAGGATCATTCTCTTCTAACACTATTAGTTTCGTCTCTATATTTTAATGTGTACGGGTGTGTCATATCATGCCTGAATTTATGAATTATTAAAAATAATTTTTTATACGAAGACATGAAATAAGACCCGAAAAAAAAAAAGAAAGAAAATTTGGAGGATTTAAATCTAATTTTTAATGTTATTAAAAAATATTCAATATCTTTTTCTCTTTCTCCCTCTTTTTTTTATTTATATTTTTCTTTTCCAAAATGGTTAAGGGATATAGAATTTGTTGGAAAATTAATTTGAAAATAATTTAGCTTGTTTAGGTTGTACTACTGTACTAAAAAAAAAGTTGTATTACTGTATACAACTAACAGTTATATGAATCTTCAGTAAAATCTATAAATAATTAATTGAAATGTTTTTCTGCGAGGCTTTTTTCTTTTTCTCCCTCTCTTTTCTTCTTCTTCGGATGGCAAACTTATTTCTGCAAGTGACCCTCGGTTAAATTTCTACAGGTCTTTTCAGGTCCGACCTCAACAATTGTTCATGCAAACATATTGGAAGTTCAAACAAACCTTGCAAGATTTTAGGTCCATTTATTAACCAATTTGTGTTTTTTGTTTTGTTTTGTTTTTTTAATTTTCTGGTTTTGAAAATAACTATAATTTTGTTTACTATATTACTGAAGTTTTGTTTGATAATTATCTATTTTTCACTTTTATATATATATATATATCTTCTTCTTTCAAGTCAAATTAAGAAAAGAAATAAAAGTTTTAAAAAAAATTTTGTCTTTTAAATTTAACTAAAAATTCAAATGCCAATGAAACTGATGGTAAAGAAATTCAGAAAAATAACAAACATATCAAAAATAAGAAATAAAAATTCAAATGGTATCAAATGAGATATTACGTTTTTTAAAATGTTTTAGAAAACTAAGCTGAATTTTAAAAACTAAAAAAAATAGTTTTTAAAATTTTGATTTTATTTTTAAAATTTAGTTTAGAATTTAAATGTATTTAAAAAAAAAAACAAGTGAAAAGCTTATCTAAAAATGGGAGGGAACAAACACAATTTTCAAAAATAGAAAACAAAAGAAAGGGGAAATTTTCCAACCTTCCAACCGCCAACAGCTTTGAACCTCTGGGGAATTTCCTCCTCTTCTCTTCAAGCTCACGGTTCAGCTTAAAGCGCCTGTAATTCATGAAGGGAAGGTTCCATTCCATCCAGGTATCTCTTGAATCCTTGTTCTCGAGTCAACTCACATGGGAAGTCCACTATTGGTTTCGCATTGTGATGTTCAAGAAAAGCAAAGAAAACTTGCATTTACTAAGTATTGTCTCTTAGCACAGCATTTAAGGAAGCATGGATTACTCAATCTAGCGAAAGTGGGTATAGAATATGCATTTGTGAATGTTTATGCTACAATTAATGGAGAAAGTAGCTGGCTAGCTGTTGTGTGTACTTGAAGTTATCTTGTTGACATTTACTTACAATTTGACAAGTTGCAGATTTCACCATTCCAAAACAGACTCAACGTTTTTCTTCATTGTTCATCACCAAAATCTATGGTCTATCTGGACCCTATATATATGGACTTGGCTCTCTTCTCTTTTCACAATTGAGTTGTTGTTGTTCAAAGCCAATGGCTTGCAAGTTCCTGTTTCCTCCGTTAGTAGCTTCGTTTCTAATACTTTTAACCCTTCTTAGCTCATCATCTGCAGCAGATGATTCTCTCACTAATTTTTCCCCTGCAAAAGCCATTTGCAAATTCACCTCAAATCCTTCTTACTGCATCTCTGTGCTTCAAAAATCCACAGGCAATGTCTACGAGTCAGGTCGGTTTTCGATGCGTCGGTCTTTGTCCAAAGCATCTAGATTCTTTGACTTGATCGAGAAGCATCTTCAAAACAGTTCAACTCTACCAAAATCCGTCACTGGAGCTCTTACAGATTGCCAGTATCTTGCCCAACTAAACATCAATTTCTTGTCTAATTCATTTCGGGCCGTCAATGGCACTGATAAAAAGCTAACTTACTCAAAAGCTGATTATATCCAGAGTCTTCTGAGTGCCATCTTAACCAACATTGATACTTGTCTTGATGGCCTAAAAACTGTAGCCTCAGGTTCTAGTTTGGAGAAAGATCTCTTAGCCCCACTCATTGACTGTACCAAATCCTACAGTCTTTCTCTGGATCTTTTTACAAAGGGCTGGGTGCCTAGGAATAGAAACAGAACACTTGAGCACCCTGGGAGAAAACACCTCCAGTTTAGGAAGGGACCGTTACCGTTGAGAATGTCGAGACACGACCGTGCGGTTTATAACTCGGTGGCGAATCGGAGAAGGCTTACATCTTCATCAGATGATGGAGTCTTGATTAATGGCATTGTAGTTGTGAGTCAAGATGGCACAGGGGACTTCCTCACCATCACTGACGCTGTTAATGCATCTCCAAACAACTCTCTTGCTGCCAATGGCTACTTCTTGATTTACATCACGGCTGGTGTTTACCAAGAGTATGTGTCGATCCCTTCCAAGAAGAAGTACTTGCTGATGATTGGAGATGGTATAAATCAGACTATCATTACGGGCAACCGAAGCGTCGTGGACGGGTGGACGACTTTCAATAGTGCAACTTTTGGTGAGCATTTAAATGTTCTTTTAACTTTGTGGTCAATAGGTCTCTAAAATTAAAAAATTGTCTAATAAATTCCTAAACTTTCAAAACTTTCAATTTTATGTTTAATAGGTTAAGTTCAGGACGAAAGTACCAAACTTATAATTTGACCTTAATTTAAATAAAAATAACCATTTCAATTAAGTCAATCCATAAAATTTAGTCAACAATAGCCTTTTGAGATTGGTGCCTCCTCTAACAAGCTTTTAAAATTTATCATTTTATCCTGCATTTCCAAATCTGAAACTCACCAAACAGAAACACAAAACTACTCAACTGGTGTGCTATTATCTTAACCAACTCAAACAAAATCCAAGTTTTAAAATGTCATTTGCTTATGACTGATGCAGCTGTGGCAGCAGAAGGATTCATGGCAGTGAACATAACCTTTCAAAACACAGCTGGAGCAATCAAAGGCCAAGCAGTTGCAGTAAGAAGCGGCGCCGATATGGCTGTCTTCTACAGCTGCAGCTTTGAAGGCTACCAAGACACCTTATACACACACTCCCTCAGACAATTCTTCAGAGAATGTGACATCTATGGCACAGTGGACTTCATATTTGGAAATGCAGCAGTGGTGTTCCAAAACTGCAACATATATCCCCGTCTACCGCGTCCAGGACAGAGCAACATGATCACGGCTCAAGGTCGATCGGACCCGAACCAAAACACCGGGACTTCGATCCACAACTGCACGATCCGAGCCACGAGCGACTTGGCTGCGAGCAACAGCTACATCAACAAGACATATTTGGGAAGACCATGGAAGCAATATTCAAGAACAGTGTATATGCAGACATTCATTGATGGGTTTATAAATCCTGGTGGTTGGGATCCTTGGTCAAGTGAATATCTAAGCACTTTATATTATGCTGAGTATAACAATACAGGAGCTGGATCAAATACAAAGAACAGAGTTACCTGGCCTGGTTACCATGTGATTTATAATGCTACAGATGCTTCTAATTTTACAGTTTCAAACTTCTTGGTTGGGGATGTGTGGTTGCCTCCAACTGATGTGCCTTACACTGGAGGCTTTGTATAA

mRNA sequence

ATGATTTCTCCTTGCCAGTCCCCAGATCGATGGTGTGTGATTTGCTCCAAAAGTTTTCGGTTTCTGCGTCCTAACGACGACAGATCGAAAGCTCAAGAAACCACCGCACCACCGATCAGCGCAACATTTCGACGACGGGCAGAGGGAGCAATGCGTTTCGGTACGATCCCTTTTCCCCTTGTTTGTTTTCGGGAAGTTGAGGATTCATGGCTAATTTTGCTTCTTCTCTCCTTTGCATGGATCAAATTTGCTCCGATCAGATCGCTGGCTGTTGGTTATCACACAACAATCAAGGTTTCTGTATGGTACTCAGAGCTCACGGTTCAGCTTAAAGCGCCTGTAATTCATGAAGGGAAGGTTCCATTCCATCCAGATTTCACCATTCCAAAACAGACTCAACGTTTTTCTTCATTGTTCATCACCAAAATCTATGGTCTATCTGGACCCTATATATATGGACTTGGCTCTCTTCTCTTTTCACAATTGAGTTGTTGTTGTTCAAAGCCAATGGCTTGCAAGTTCCTGTTTCCTCCGTTAGTAGCTTCGTTTCTAATACTTTTAACCCTTCTTAGCTCATCATCTGCAGCAGATGATTCTCTCACTAATTTTTCCCCTGCAAAAGCCATTTGCAAATTCACCTCAAATCCTTCTTACTGCATCTCTGTGCTTCAAAAATCCACAGGCAATGTCTACGAGTCAGGTCGGTTTTCGATGCGTCGGTCTTTGTCCAAAGCATCTAGATTCTTTGACTTGATCGAGAAGCATCTTCAAAACAGTTCAACTCTACCAAAATCCGTCACTGGAGCTCTTACAGATTGCCAGTATCTTGCCCAACTAAACATCAATTTCTTGTCTAATTCATTTCGGGCCGTCAATGGCACTGATAAAAAGCTAACTTACTCAAAAGCTGATTATATCCAGAGTCTTCTGAGTGCCATCTTAACCAACATTGATACTTGTCTTGATGGCCTAAAAACTGTAGCCTCAGGTTCTAGTTTGGAGAAAGATCTCTTAGCCCCACTCATTGACTGTACCAAATCCTACAGTCTTTCTCTGGATCTTTTTACAAAGGGCTGGGTGCCTAGGAATAGAAACAGAACACTTGAGCACCCTGGGAGAAAACACCTCCAGTTTAGGAAGGGACCGTTACCGTTGAGAATGTCGAGACACGACCGTGCGGTTTATAACTCGGTGGCGAATCGGAGAAGGCTTACATCTTCATCAGATGATGGAGTCTTGATTAATGGCATTGTAGTTGTGAGTCAAGATGGCACAGGGGACTTCCTCACCATCACTGACGCTGTTAATGCATCTCCAAACAACTCTCTTGCTGCCAATGGCTACTTCTTGATTTACATCACGGCTGGTGTTTACCAAGAGTATGTGTCGATCCCTTCCAAGAAGAAGTACTTGCTGATGATTGGAGATGGTATAAATCAGACTATCATTACGGGCAACCGAAGCGTCGTGGACGGGTGGACGACTTTCAATAGTGCAACTTTTGCTGTGGCAGCAGAAGGATTCATGGCAGTGAACATAACCTTTCAAAACACAGCTGGAGCAATCAAAGGCCAAGCAGTTGCAGTAAGAAGCGGCGCCGATATGGCTGTCTTCTACAGCTGCAGCTTTGAAGGCTACCAAGACACCTTATACACACACTCCCTCAGACAATTCTTCAGAGAATGTGACATCTATGGCACAGTGGACTTCATATTTGGAAATGCAGCAGTGGTGTTCCAAAACTGCAACATATATCCCCGTCTACCGCGTCCAGGACAGAGCAACATGATCACGGCTCAAGGTCGATCGGACCCGAACCAAAACACCGGGACTTCGATCCACAACTGCACGATCCGAGCCACGAGCGACTTGGCTGCGAGCAACAGCTACATCAACAAGACATATTTGGGAAGACCATGGAAGCAATATTCAAGAACAGTGTATATGCAGACATTCATTGATGGGTTTATAAATCCTGGTGGTTGGGATCCTTGGTCAAGTGAATATCTAAGCACTTTATATTATGCTGAGTATAACAATACAGGAGCTGGATCAAATACAAAGAACAGAGTTACCTGGCCTGGTTACCATGTGATTTATAATGCTACAGATGCTTCTAATTTTACAGTTTCAAACTTCTTGGTTGGGGATGTGTGGTTGCCTCCAACTGATGTGCCTTACACTGGAGGCTTTGTATAA

Coding sequence (CDS)

ATGATTTCTCCTTGCCAGTCCCCAGATCGATGGTGTGTGATTTGCTCCAAAAGTTTTCGGTTTCTGCGTCCTAACGACGACAGATCGAAAGCTCAAGAAACCACCGCACCACCGATCAGCGCAACATTTCGACGACGGGCAGAGGGAGCAATGCGTTTCGGTACGATCCCTTTTCCCCTTGTTTGTTTTCGGGAAGTTGAGGATTCATGGCTAATTTTGCTTCTTCTCTCCTTTGCATGGATCAAATTTGCTCCGATCAGATCGCTGGCTGTTGGTTATCACACAACAATCAAGGTTTCTGTATGGTACTCAGAGCTCACGGTTCAGCTTAAAGCGCCTGTAATTCATGAAGGGAAGGTTCCATTCCATCCAGATTTCACCATTCCAAAACAGACTCAACGTTTTTCTTCATTGTTCATCACCAAAATCTATGGTCTATCTGGACCCTATATATATGGACTTGGCTCTCTTCTCTTTTCACAATTGAGTTGTTGTTGTTCAAAGCCAATGGCTTGCAAGTTCCTGTTTCCTCCGTTAGTAGCTTCGTTTCTAATACTTTTAACCCTTCTTAGCTCATCATCTGCAGCAGATGATTCTCTCACTAATTTTTCCCCTGCAAAAGCCATTTGCAAATTCACCTCAAATCCTTCTTACTGCATCTCTGTGCTTCAAAAATCCACAGGCAATGTCTACGAGTCAGGTCGGTTTTCGATGCGTCGGTCTTTGTCCAAAGCATCTAGATTCTTTGACTTGATCGAGAAGCATCTTCAAAACAGTTCAACTCTACCAAAATCCGTCACTGGAGCTCTTACAGATTGCCAGTATCTTGCCCAACTAAACATCAATTTCTTGTCTAATTCATTTCGGGCCGTCAATGGCACTGATAAAAAGCTAACTTACTCAAAAGCTGATTATATCCAGAGTCTTCTGAGTGCCATCTTAACCAACATTGATACTTGTCTTGATGGCCTAAAAACTGTAGCCTCAGGTTCTAGTTTGGAGAAAGATCTCTTAGCCCCACTCATTGACTGTACCAAATCCTACAGTCTTTCTCTGGATCTTTTTACAAAGGGCTGGGTGCCTAGGAATAGAAACAGAACACTTGAGCACCCTGGGAGAAAACACCTCCAGTTTAGGAAGGGACCGTTACCGTTGAGAATGTCGAGACACGACCGTGCGGTTTATAACTCGGTGGCGAATCGGAGAAGGCTTACATCTTCATCAGATGATGGAGTCTTGATTAATGGCATTGTAGTTGTGAGTCAAGATGGCACAGGGGACTTCCTCACCATCACTGACGCTGTTAATGCATCTCCAAACAACTCTCTTGCTGCCAATGGCTACTTCTTGATTTACATCACGGCTGGTGTTTACCAAGAGTATGTGTCGATCCCTTCCAAGAAGAAGTACTTGCTGATGATTGGAGATGGTATAAATCAGACTATCATTACGGGCAACCGAAGCGTCGTGGACGGGTGGACGACTTTCAATAGTGCAACTTTTGCTGTGGCAGCAGAAGGATTCATGGCAGTGAACATAACCTTTCAAAACACAGCTGGAGCAATCAAAGGCCAAGCAGTTGCAGTAAGAAGCGGCGCCGATATGGCTGTCTTCTACAGCTGCAGCTTTGAAGGCTACCAAGACACCTTATACACACACTCCCTCAGACAATTCTTCAGAGAATGTGACATCTATGGCACAGTGGACTTCATATTTGGAAATGCAGCAGTGGTGTTCCAAAACTGCAACATATATCCCCGTCTACCGCGTCCAGGACAGAGCAACATGATCACGGCTCAAGGTCGATCGGACCCGAACCAAAACACCGGGACTTCGATCCACAACTGCACGATCCGAGCCACGAGCGACTTGGCTGCGAGCAACAGCTACATCAACAAGACATATTTGGGAAGACCATGGAAGCAATATTCAAGAACAGTGTATATGCAGACATTCATTGATGGGTTTATAAATCCTGGTGGTTGGGATCCTTGGTCAAGTGAATATCTAAGCACTTTATATTATGCTGAGTATAACAATACAGGAGCTGGATCAAATACAAAGAACAGAGTTACCTGGCCTGGTTACCATGTGATTTATAATGCTACAGATGCTTCTAATTTTACAGTTTCAAACTTCTTGGTTGGGGATGTGTGGTTGCCTCCAACTGATGTGCCTTACACTGGAGGCTTTGTATAA

Protein sequence

MISPCQSPDRWCVICSKSFRFLRPNDDRSKAQETTAPPISATFRRRAEGAMRFGTIPFPLVCFREVEDSWLILLLLSFAWIKFAPIRSLAVGYHTTIKVSVWYSELTVQLKAPVIHEGKVPFHPDFTIPKQTQRFSSLFITKIYGLSGPYIYGLGSLLFSQLSCCCSKPMACKFLFPPLVASFLILLTLLSSSSAADDSLTNFSPAKAICKFTSNPSYCISVLQKSTGNVYESGRFSMRRSLSKASRFFDLIEKHLQNSSTLPKSVTGALTDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTNIDTCLDGLKTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTLEHPGRKHLQFRKGPLPLRMSRHDRAVYNSVANRRRLTSSSDDGVLINGIVVVSQDGTGDFLTITDAVNASPNNSLAANGYFLIYITAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNRSVVDGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAVRSGADMAVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNSYINKTYLGRPWKQYSRTVYMQTFIDGFINPGGWDPWSSEYLSTLYYAEYNNTGAGSNTKNRVTWPGYHVIYNATDASNFTVSNFLVGDVWLPPTDVPYTGGFV
Homology
BLAST of Lag0037412 vs. NCBI nr
Match: XP_038898430.1 (probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Benincasa hispida] >XP_038898431.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Benincasa hispida] >XP_038898432.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Benincasa hispida])

HSP 1 Score: 1040.4 bits (2689), Expect = 7.3e-300
Identity = 515/565 (91.15%), Postives = 542/565 (95.93%), Query Frame = 0

Query: 170 MACKFLFPPLVASFLILLTLLSSSSAADDSLTNFSPAKAICKFTSNPSYCISVLQKST-G 229
           MACKFLFPPL+ASFLILLTL +SSSA  DSLTNF P+KAICKFTSNPSYCI+VL+KST G
Sbjct: 1   MACKFLFPPLLASFLILLTLFTSSSAEYDSLTNFPPSKAICKFTSNPSYCITVLKKSTGG 60

Query: 230 NVYESGRFSMRRSLSKASRFFDLIEKHLQNSSTLPKSVTGALTDCQYLAQLNINFLSNSF 289
           N+Y+SGRFS+RRSLSKASRFFDLIEKHLQNSSTLPKS+TGALTDCQYLAQLNINFLSNSF
Sbjct: 61  NIYDSGRFSIRRSLSKASRFFDLIEKHLQNSSTLPKSITGALTDCQYLAQLNINFLSNSF 120

Query: 290 RAVNGTDKKLTYSKADYIQSLLSAILTNIDTCLDGLKTVASGSSLEKDLLAPLIDCTKSY 349
           RAVNGTDKKLTYSKADYIQSLLSAILTNIDTCLDGLKTVA+GSSLEKDLLAPLIDCTKSY
Sbjct: 121 RAVNGTDKKLTYSKADYIQSLLSAILTNIDTCLDGLKTVAAGSSLEKDLLAPLIDCTKSY 180

Query: 350 SLSLDLFTKGWVP-RNRNRTLEHPGRKHLQFRKGPLPLRMSRHDRAVYNSVANRRRLTSS 409
           SLSLDLFTKGWVP RNRNRTL+HPG+KHLQFRKGPLPLRMSR DRAVY+SVANRR+L+SS
Sbjct: 181 SLSLDLFTKGWVPRRNRNRTLDHPGKKHLQFRKGPLPLRMSRRDRAVYDSVANRRKLSSS 240

Query: 410 SDDGVLINGIVVVSQDGTGDFLTITDAVNASPNNSLAANGYFLIYITAGVYQEYVSIPSK 469
           SDDGVL+NGI VVSQDG GDFL ITDA+NA+PNNSLAANGYFLIYI+AGVYQEYVSIPSK
Sbjct: 241 SDDGVLVNGIAVVSQDGKGDFLNITDAINAAPNNSLAANGYFLIYISAGVYQEYVSIPSK 300

Query: 470 KKYLLMIGDGINQTIITGNRSVVDGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAV 529
           KKYLLMIGDGINQTIITGN SVVDGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAV
Sbjct: 301 KKYLLMIGDGINQTIITGNHSVVDGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAV 360

Query: 530 AVRSGADMAVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 589
           A+RSGADM VFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR
Sbjct: 361 ALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420

Query: 590 LPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNSYINKTYLGRPWKQYSRTV 649
           LPRPGQ+NMITAQGRSDPNQNTGTSIHNC IRATSDLAAS+SY+NKTYLGRPWKQYSRTV
Sbjct: 421 LPRPGQANMITAQGRSDPNQNTGTSIHNCAIRATSDLAASSSYMNKTYLGRPWKQYSRTV 480

Query: 650 YMQTFIDGFINPGGWDPWSSEYLSTLYYAEYNNTGAGSNTKNRVTWPGYHVIYNATDASN 709
           YMQTFIDGF+NP GWDPW+ EYLSTLYY EYNNTG GSNTKNRV W GYHVI NATDA+N
Sbjct: 481 YMQTFIDGFVNPSGWDPWTGEYLSTLYYGEYNNTGDGSNTKNRVNWAGYHVINNATDAAN 540

Query: 710 FTVSNFLVGDVWLPPTDVPYTGGFV 733
           FTVSNFLVGDVWLP T VPYTG F+
Sbjct: 541 FTVSNFLVGDVWLPQTCVPYTGAFI 565

BLAST of Lag0037412 vs. NCBI nr
Match: XP_031738568.1 (probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis sativus] >XP_031738569.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis sativus] >XP_031738570.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis sativus] >KGN60252.1 hypothetical protein Csa_001115 [Cucumis sativus])

HSP 1 Score: 1022.3 bits (2642), Expect = 2.0e-294
Identity = 502/565 (88.85%), Postives = 536/565 (94.87%), Query Frame = 0

Query: 170 MACKFLFPPLVASFLILLTLLSSSSAADDSLTNFSPAKAICKFTSNPSYCISVLQKS-TG 229
           MACKFLFPPL+ASFLILLTL +SS A+DDSLTNF P KAICK TSNPSYCI+VL++S  G
Sbjct: 1   MACKFLFPPLLASFLILLTLFTSSFASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDG 60

Query: 230 NVYESGRFSMRRSLSKASRFFDLIEKHLQNSSTLPKSVTGALTDCQYLAQLNINFLSNSF 289
           N+Y+SGRFS+RRSLSKA+RF DLIEKHLQNSSTLP S+ GAL DCQYLAQLN+NFLSNSF
Sbjct: 61  NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF 120

Query: 290 RAVNGTDKKLTYSKADYIQSLLSAILTNIDTCLDGLKTVASGSSLEKDLLAPLIDCTKSY 349
           RAVNGTD+KLTYSKADYIQSLLSAILTNIDTCLDGL TVASGSSLEKDLLAPLIDCTKSY
Sbjct: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180

Query: 350 SLSLDLFTKGWVP-RNRNRTLEHPGRKHLQFRKGPLPLRMSRHDRAVYNSVANRRRLTSS 409
           SLSLDLFTKGWVP RNRNRTLEHPG+KHLQFRKGPLPLRMSRHDRAVYNSVANRR+L+SS
Sbjct: 181 SLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS 240

Query: 410 SDDGVLINGIVVVSQDGTGDFLTITDAVNASPNNSLAANGYFLIYITAGVYQEYVSIPSK 469
           SDDGVL+NG+VVVSQDG GDFL ITDA+NA+PNNSLA++GYFLIYITAGVYQEYVS+PSK
Sbjct: 241 SDDGVLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVSVPSK 300

Query: 470 KKYLLMIGDGINQTIITGNRSVVDGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAV 529
           KKYLLMIGDGINQTIITGNRSV DGWTTFNSATFAVAAEGFMAVNIT QNTAGAIKGQAV
Sbjct: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAV 360

Query: 530 AVRSGADMAVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 589
           A+RSGADM VFYSCSFEG+QDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR
Sbjct: 361 ALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420

Query: 590 LPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNSYINKTYLGRPWKQYSRTV 649
           LPRPGQ+NMITAQGRSDPNQNTGTSIHNCTIRAT +LAAS+SY+NKTYLGRPWKQYSRTV
Sbjct: 421 LPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQYSRTV 480

Query: 650 YMQTFIDGFINPGGWDPWSSEYLSTLYYAEYNNTGAGSNTKNRVTWPGYHVIYNATDASN 709
           YMQTFIDGF+NP GWDPW+ EYLSTLYY EYNNTG GS+TKNRVTW GYHVI N TDA+N
Sbjct: 481 YMQTFIDGFVNPKGWDPWTGEYLSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNVTDAAN 540

Query: 710 FTVSNFLVGDVWLPPTDVPYTGGFV 733
           FT+SNFLVGD WLPPT VPY GGFV
Sbjct: 541 FTISNFLVGDAWLPPTWVPYMGGFV 565

BLAST of Lag0037412 vs. NCBI nr
Match: XP_016903565.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis melo] >KAA0038671.1 putative pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis melo var. makuwa] >TYK31280.1 putative pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1021.1 bits (2639), Expect = 4.6e-294
Identity = 506/565 (89.56%), Postives = 534/565 (94.51%), Query Frame = 0

Query: 170 MACKFLFPPLVASFLILLTLLSSSSAADDSLTNFSPAKAICKFTSNPSYCISVLQKS-TG 229
           MACKFLFPPL   FLILLTL +SS A+DDSLTN  P KAICK TSNPSYCI+VL++S  G
Sbjct: 1   MACKFLFPPL---FLILLTLFTSSFASDDSLTNIFPTKAICKLTSNPSYCITVLKQSRDG 60

Query: 230 NVYESGRFSMRRSLSKASRFFDLIEKHLQNSSTLPKSVTGALTDCQYLAQLNINFLSNSF 289
           N+Y+SGRFS+RRSLSKASRF DLIEKHLQNSSTLPKS+ GAL DCQYLAQLN+NFLSNSF
Sbjct: 61  NIYDSGRFSIRRSLSKASRFLDLIEKHLQNSSTLPKSIIGALKDCQYLAQLNMNFLSNSF 120

Query: 290 RAVNGTDKKLTYSKADYIQSLLSAILTNIDTCLDGLKTVASGSSLEKDLLAPLIDCTKSY 349
           RAVNGTD+KLTYSKADYIQSLLSAILTNIDTCLDGL TVASGSSLEKDLLAPLIDCTKSY
Sbjct: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180

Query: 350 SLSLDLFTKGWVPR-NRNRTLEHPGRKHLQFRKGPLPLRMSRHDRAVYNSVANRRRLTSS 409
           SLSLDLFTKGWVPR NRNRTLEHPG+KHLQFRKGPLPLRMSRHDRAVYNSVANRR+L+SS
Sbjct: 181 SLSLDLFTKGWVPRWNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS 240

Query: 410 SDDGVLINGIVVVSQDGTGDFLTITDAVNASPNNSLAANGYFLIYITAGVYQEYVSIPSK 469
           SDDGVL+NGI VVSQDG GDFL ITDA+NA+PNNSLAANGYFLIYITAGVYQEYVS+PSK
Sbjct: 241 SDDGVLVNGIAVVSQDGEGDFLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSK 300

Query: 470 KKYLLMIGDGINQTIITGNRSVVDGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAV 529
           KKYLLMIGDGINQTIITGNRSV DGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAV
Sbjct: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAV 360

Query: 530 AVRSGADMAVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 589
           A+RSGADM VFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR
Sbjct: 361 ALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420

Query: 590 LPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNSYINKTYLGRPWKQYSRTV 649
           LP P Q+NMITAQGRSDPNQNTGTSIHNCTI+AT DLAAS+S++NKTYLGRPWKQYSRTV
Sbjct: 421 LPGPRQANMITAQGRSDPNQNTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTV 480

Query: 650 YMQTFIDGFINPGGWDPWSSEYLSTLYYAEYNNTGAGSNTKNRVTWPGYHVIYNATDASN 709
           YMQTFIDGF+NP GWDPWS EYLSTLYY EYNNTG GS+TKNRV WPGYHVI NATDA+N
Sbjct: 481 YMQTFIDGFVNPKGWDPWSGEYLSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAAN 540

Query: 710 FTVSNFLVGDVWLPPTDVPYTGGFV 733
           FT+SNFLVGDVWLPPT VPYTGGFV
Sbjct: 541 FTISNFLVGDVWLPPTGVPYTGGFV 562

BLAST of Lag0037412 vs. NCBI nr
Match: XP_023524144.1 (probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023524145.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023524146.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1007.3 bits (2603), Expect = 6.8e-290
Identity = 494/536 (92.16%), Postives = 513/536 (95.71%), Query Frame = 0

Query: 197 DDSLTNFSPAKAICKFTSNPSYCISVLQKSTGNVYESGRFSMRRSLSKASRFFDLIEKHL 256
           D SL +  PAKAICKFTSNPSYCISVLQKS+GNVYESGRFSMRRSLSKAS F +LIEKHL
Sbjct: 8   DGSLNDLFPAKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLSKASAFLNLIEKHL 67

Query: 257 QNSSTLPKSVTGALTDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTN 316
           QNSSTLPKSVTG L DCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTN
Sbjct: 68  QNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTN 127

Query: 317 IDTCLDGLKTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTLEHPGRKHL 376
           IDTCLDGL TVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRT +HP  KHL
Sbjct: 128 IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRDHPVTKHL 187

Query: 377 QFRKGPLPLRMSRHDRAVYNSVANRRRLTSSSDDGVLINGIVVVSQDGTGDFLTITDAVN 436
           +FRKGPLPLRMS+HDR VYNSVANRRRL+SSSDDG+L+NGIVVVSQDGTG+FLTIT+AVN
Sbjct: 188 RFRKGPLPLRMSKHDRVVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFLTITEAVN 247

Query: 437 ASPNNSLAANGYFLIYITAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNRSVVDGWTTF 496
           A+PNNSLA NGYFLIYI AGVYQEYVSIPSKKKYLLMIGDGINQTIITGN+SV DGWTTF
Sbjct: 248 AAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSVADGWTTF 307

Query: 497 NSATFAVAAEGFMAVNITFQNTAGAIKGQAVAVRSGADMAVFYSCSFEGYQDTLYTHSLR 556
           NSATFAVAAEGFMAVNITFQNTAGAIKGQAVA+RSGADM++FYSCSFEGYQDTLYTHSLR
Sbjct: 308 NSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDTLYTHSLR 367

Query: 557 QFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQSNMITAQGRSDPNQNTGTSIHNC 616
           QFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPR GQ+NMITAQGRSDPNQNTGTSIHNC
Sbjct: 368 QFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNTGTSIHNC 427

Query: 617 TIRATSDLAASNSYINKTYLGRPWKQYSRTVYMQTFIDGFINPGGWDPWSSEYLSTLYYA 676
           TIRATSDLAAS SY+NKTYLGRPWKQYSRTVYMQTFIDGFINP GWDPWS EYLSTLYYA
Sbjct: 428 TIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFINPAGWDPWSGEYLSTLYYA 487

Query: 677 EYNNTGAGSNTKNRVTWPGYHVIYNATDASNFTVSNFLVGDVWLPPTDVPYTGGFV 733
           EYNNTGAGSNTKNRVTWPGYHVI NATDASNFTVSNFLVGD WLPPT VPYTGGF+
Sbjct: 488 EYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTGGFI 543

BLAST of Lag0037412 vs. NCBI nr
Match: KAG6608276.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1005.7 bits (2599), Expect = 2.0e-289
Identity = 493/536 (91.98%), Postives = 513/536 (95.71%), Query Frame = 0

Query: 197 DDSLTNFSPAKAICKFTSNPSYCISVLQKSTGNVYESGRFSMRRSLSKASRFFDLIEKHL 256
           D SL    PAKAICKFTSNPSYCISVLQKS+GNVYESGRFSMRRSLSKA+ F +LIEKHL
Sbjct: 8   DGSLNGLFPAKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLSKATTFLNLIEKHL 67

Query: 257 QNSSTLPKSVTGALTDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTN 316
           QNSSTLPKSVTG L DCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTN
Sbjct: 68  QNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTN 127

Query: 317 IDTCLDGLKTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTLEHPGRKHL 376
           IDTCLDGL TVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRT +HP  KHL
Sbjct: 128 IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRDHPVTKHL 187

Query: 377 QFRKGPLPLRMSRHDRAVYNSVANRRRLTSSSDDGVLINGIVVVSQDGTGDFLTITDAVN 436
           +FRKGPLPLRMS+HDRAVYNSVANRRRL+SSSDDG+L+NGIVVVSQDGTG+FLTIT+AVN
Sbjct: 188 RFRKGPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFLTITEAVN 247

Query: 437 ASPNNSLAANGYFLIYITAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNRSVVDGWTTF 496
           A+PNNSLA NGYFLIYI AGVYQEYVSIPSKKKYLLMIGDGINQTIITGN+SV DGWTTF
Sbjct: 248 AAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSVADGWTTF 307

Query: 497 NSATFAVAAEGFMAVNITFQNTAGAIKGQAVAVRSGADMAVFYSCSFEGYQDTLYTHSLR 556
           NSATFAVAAEGFMAVNITFQNTAGAIKGQAVA+RSGADM++FYSCSFEGYQDTLYTHSLR
Sbjct: 308 NSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDTLYTHSLR 367

Query: 557 QFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQSNMITAQGRSDPNQNTGTSIHNC 616
           QFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPR GQ+NMITAQGRSDPNQNTGTSIHNC
Sbjct: 368 QFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNTGTSIHNC 427

Query: 617 TIRATSDLAASNSYINKTYLGRPWKQYSRTVYMQTFIDGFINPGGWDPWSSEYLSTLYYA 676
           TIRATSDLAAS SY+NKTYLGRPWKQYSRTVYMQTFIDGFINP GWDPWS EYLSTLYYA
Sbjct: 428 TIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFINPAGWDPWSGEYLSTLYYA 487

Query: 677 EYNNTGAGSNTKNRVTWPGYHVIYNATDASNFTVSNFLVGDVWLPPTDVPYTGGFV 733
           EYNN+GAGSNTKNRVTWPGYHVI NATDASNFTVSNFLVGD WLPPT VPYTGGF+
Sbjct: 488 EYNNSGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTGGFI 543

BLAST of Lag0037412 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 642.5 bits (1656), Expect = 5.8e-183
Identity = 332/561 (59.18%), Postives = 412/561 (73.44%), Query Frame = 0

Query: 183 FLILLTLLSS-----SSAADDSLTNFSPAKAICKFTSNPSYCISVLQKSTGNVYESGRFS 242
           FL  L  LSS      SA   ++ N +    IC+F  +PSYC SVL    G++Y  GR S
Sbjct: 7   FLFTLACLSSLPSPFISAQIPAIGNATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLS 66

Query: 243 MRRSLSKASRFFDLIEKHL-QNSSTLPKSVTGALTDCQYLAQLNINFLSNSFRAVNGTDK 302
           +RRSLS+A RF  +I+  L +      KS  GAL DC++LA L +++L +S +  + T K
Sbjct: 67  LRRSLSRARRFISMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADST-K 126

Query: 303 KLTYSKADYIQSLLSAILTNIDTCLDGLKTVASGSSLEKDLLAPLIDCTKSYSLSLDLFT 362
            L+ S+A+ + + LSA +TN  TCL+GLK+ AS + L  D    L + TK Y +SL LF+
Sbjct: 127 TLSLSRAEDVHTFLSAAITNEQTCLEGLKSTASENGLSGD----LFNDTKLYGVSLALFS 186

Query: 363 KGWVP-RNRNRTLEHPG---RKHLQFRKGPLPLRMSRHDRAVYNSVANRRRLTSSSDDGV 422
           KGWVP R R+R +  P    +K   FR G LPL+M+   RAVYN+V  RR+L  S  D V
Sbjct: 187 KGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTV-TRRKLLQSDADAV 246

Query: 423 LINGIVVVSQDGTGDFLTITDAVNASPNNSLAANGYFLIYITAGVYQEYVSIPSKKKYLL 482
            ++ IV V Q+GTG+F TI  A+ A+PN +  +NGYFLIY+TAG+Y+EYV +P  K+Y++
Sbjct: 247 QVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVM 306

Query: 483 MIGDGINQTIITGNRSVVDGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAVRSG 542
           MIGDGINQT+ITGNRSVVDGWTTFNSATF ++   F+ VNIT +NTAG  KGQAVA+RSG
Sbjct: 307 MIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSG 366

Query: 543 ADMAVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPG 602
            D++VFYSCSFE YQDTLYTHSLRQF+RECD+YGTVDFIFGNAAVV QNCN+YPR PR G
Sbjct: 367 GDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKG 426

Query: 603 QSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNSYINKTYLGRPWKQYSRTVYMQTF 662
           QSN +TAQGR+DPNQNTGT+IH CTIR   DLA SN Y  KTYLGRPWK+YSRTV MQT+
Sbjct: 427 QSNEVTAQGRTDPNQNTGTAIHGCTIRPADDLATSN-YTVKTYLGRPWKEYSRTVVMQTY 486

Query: 663 IDGFINPGGWDPWSSEY-LSTLYYAEYNNTGAGSNTKNRVTWPGYHVIYNATDASNFTVS 722
           IDGF+ P GW+ WS ++ LSTLYYAEYNNTG GS+T NRVTWPGYHVI NATDASNFTV+
Sbjct: 487 IDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVI-NATDASNFTVT 546

Query: 723 NFLVGDVWLPPTDVPYTGGFV 733
           NFLVG+ W+  T VP+ GG +
Sbjct: 547 NFLVGEGWIGQTGVPFVGGLI 559

BLAST of Lag0037412 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 622.5 bits (1604), Expect = 6.2e-177
Identity = 326/571 (57.09%), Postives = 418/571 (73.20%), Query Frame = 0

Query: 179 LVASFLILLTLLSSSSAADDSLTNFSPAKAICKFTSNPSYCISVL-QKSTGNVYESGRFS 238
           LV  FL L TL      A  +L   + +  ICK T +P +C SV  Q S G+V E GRFS
Sbjct: 8   LVTLFLSLQTLF----IASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFS 67

Query: 239 MRRSLSKASRFFDLIEKHL-QNSSTLPKSVTGALTDCQYLAQLNINFLSNSFRAVNGT-- 298
           +R+SL+++ +F   I+++L +N++ L +S  GAL DC+YLA L  ++L  SF  VN T  
Sbjct: 68  LRKSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTS 127

Query: 299 DKKLTYSKADYIQSLLSAILTNIDTCLDGLKTVASGS-SLEKDLLAPLIDCTKSYSLSLD 358
            K L++SKAD IQ+LLSA LTN  TCLDG+ T AS S ++   +  PLI+ TK +S+SL 
Sbjct: 128 SKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLA 187

Query: 359 LFTKGWVPRNRNRTLE----HPGRKH------LQFRKGPLPLRMSRHDRAVYNSVANRRR 418
           LFTKGWVP+ + +       HP   H        FR G LPL+M+ H RAVY S++ R+ 
Sbjct: 188 LFTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKL 247

Query: 419 LTSSSD-DGVLINGIVVVSQDGTGDFLTITDAVNASPNNSLAANGYFLIYITAGVYQEYV 478
               +D + VL++ IV V+Q+GTG+F TIT+AVN++PN +    GYF+IY+T+GVY+E V
Sbjct: 248 ADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENV 307

Query: 479 SIPSKKKYLLMIGDGINQTIITGNRSVVDGWTTFNSATFAVAAEGFMAVNITFQNTAGAI 538
            I   K+YL+MIGDGIN+T++TGNR+VVDGWTTFNSATFAV +  F+AVN+TF+NTAG  
Sbjct: 308 VIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPE 367

Query: 539 KGQAVAVRSGADMAVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNC 598
           K QAVA+RS AD+++FYSCSFE YQDTLYTHSLRQF+RECDIYGTVDFIFGNAAVVFQ+C
Sbjct: 368 KHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDC 427

Query: 599 NIYPRLPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNSYINKTYLGRPWKQ 658
           N+YPR P   Q N ITAQGR+DPNQNTG SIHNCTI+   DL +SN Y  KTYLGRPWK+
Sbjct: 428 NLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSN-YTVKTYLGRPWKE 487

Query: 659 YSRTVYMQTFIDGFINPGGWDPWSSEY-LSTLYYAEYNNTGAGSNTKNRVTWPGYHVIYN 718
           YSRTV+MQ++ID  + P GW  W+ ++ LSTLYYAEYNNTG+GS+T +RV WPGYHVI N
Sbjct: 488 YSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVI-N 547

Query: 719 ATDASNFTVSNFLVGDVWLPPTDVPYTGGFV 733
           +TDA+NFTV NFL+GD W+  + VPY  G +
Sbjct: 548 STDANNFTVENFLLGDGWMVQSGVPYISGLL 572

BLAST of Lag0037412 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 1.4e-176
Identity = 334/580 (57.59%), Postives = 416/580 (71.72%), Query Frame = 0

Query: 185 ILLTL---LSSSSAADDSLTNFSPAKAICKFTSNPSYCISVLQKSTGNVYESGRFSMRRS 244
           IL+TL   L S  A+  +L+N S    ICK T +P YC SV   S GNV + G FS+R+S
Sbjct: 7   ILITLSFFLQSVLASSQTLSNSS---TICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKS 66

Query: 245 LSKASRFFDLIEKHLQNSSTLPK-SVTGALTDCQYLAQLNINFLSNSFRAVNGTD----- 304
           LS++ +F   ++++++ ++ L + +V  AL DC++LA L +++L  SF  VN T      
Sbjct: 67  LSQSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSF 126

Query: 305 KKLTYSKADYIQSLLSAILTNIDTCLDGLKTVASGS---SLEKDLLAPLIDCTKSYSLSL 364
           K L++ KAD IQ+LLSA LTN  TCL+GL T AS S   ++   +  PL++ TK   +SL
Sbjct: 127 KPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSL 186

Query: 365 DLFTKGWVPRNRNR-------------TLEHPGRKHLQFRKGPLPLRMSRHDRAVYNSVA 424
            LFTKGWVP+ + R             T   P R    FR G LPL+M+   +AVY S++
Sbjct: 187 ALFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFR---LFRNGALPLKMTEKTKAVYESLS 246

Query: 425 NRRRLTSSS----DDG--VLINGIVVVSQDGTGDFLTITDAVNASPNNSLAANGYFLIYI 484
            R+     S    DDG  VLI+ IV VSQDGTG+F  IT AV A+PNN+  + G+FLIY+
Sbjct: 247 RRKLADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYV 306

Query: 485 TAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNRSVVDGWTTFNSATFAVAAEGFMAVNI 544
           TAG+Y+EY+SI   K+Y++MIGDGINQT++TGNRSVVDGWTTFNSATFAV A  F+AVNI
Sbjct: 307 TAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNI 366

Query: 545 TFQNTAGAIKGQAVAVRSGADMAVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFG 604
           TF+NTAG  K QAVA+RSGAD ++FYSCSFE YQDTLYTHSLRQF+RECD+YGTVDFIFG
Sbjct: 367 TFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFG 426

Query: 605 NAAVVFQNCNIYPRLPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNSYINK 664
           NAAVVFQNCN+YPR P P Q N ITAQGRSDPNQNTGTSI NCTI+   DL +SN Y  K
Sbjct: 427 NAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSN-YTVK 486

Query: 665 TYLGRPWKQYSRTVYMQTFIDGFINPGGWDPWSSEY-LSTLYYAEYNNTGAGSNTKNRVT 724
           TYLGRPWK+YSRTVYMQ++IDGF+ P GW  W+ ++ LSTLYYAEYNNTG GSNT NRVT
Sbjct: 487 TYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVT 546

Query: 725 WPGYHVIYNATDASNFTVSNFLVGDVWLPPTDVPYTGGFV 733
           WPGYHVI N+TDA+NFTV+   +   W+  T VPYT G +
Sbjct: 547 WPGYHVI-NSTDAANFTVTGLFIEADWIWKTGVPYTSGLI 578

BLAST of Lag0037412 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 1.5e-127
Identity = 251/550 (45.64%), Postives = 347/550 (63.09%), Query Frame = 0

Query: 204 SPAKAICKFTSNPSYCISVL---QKSTGNVYESGRFSMRRSLSKASRFFDLIEKHLQNSS 263
           SP+ A CK T  P  C ++L   + S  + Y  G+F++++ L +ASR   +I  + +   
Sbjct: 78  SPSLA-CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVE 137

Query: 264 TLPKSVT----GALTDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTN 323
           + P S T    GA+ DC  L++L++N+L      +  T + +T +  +++ SLLS ++TN
Sbjct: 138 SKPGSATAEEIGAVADCGELSELSVNYLETVTTELK-TAQVMTAALVEHVNSLLSGVVTN 197

Query: 324 IDTCLDGLKTVASGSSLEKDLLAPLIDCTKSYSLSLDLF-------------TKGWVPRN 383
             TCLDGL    SG +    + +P+ + T+ YS+SL L              +KG +   
Sbjct: 198 QQTCLDGLVEAKSGFAAA--IGSPMGNLTRLYSISLGLVSHALNRNLKRFKASKGKILGG 257

Query: 384 RNRTLEHPGRKHLQFRKGPLPLRMSRHDRAVYNSVANRRRLTSSSDDGVLINGIVVVSQD 443
            N T   P    ++  +     +   +D+    +  N   L  +S   +L++  V+V   
Sbjct: 258 GNSTYREPLETLIKGLR-----KTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPF 317

Query: 444 GTGDFLTITDAVNASPNNSLAANGYFLIYITAGVYQEYVSIPSKKKYLLMIGDGINQTII 503
            + +F TITDA+ A+PNN+   +GYF+IY   GVY+EY+ +P  KK L+++GDGIN+TII
Sbjct: 318 KSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTII 377

Query: 504 TGNRSVVDGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAVRSGADMAVFYSCSF 563
           TGN +VVDGWTT+N ++FAV  E FMAV++TF+NTAG  K QAVA+R+ A+ + FY CSF
Sbjct: 378 TGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSF 437

Query: 564 EGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQSNMITAQGRS 623
           EGYQDTLY HSLRQF+RECDIYGTVDFIFGNAA +FQNCNIY R P   Q N ITA GR 
Sbjct: 438 EGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRL 497

Query: 624 DPNQNTGTSIHNCTIRATSDLAASNSYINKTYLGRPWKQYSRTVYMQTFIDGFINPGGWD 683
           DPNQNTG SI NCTI+A  DLAA       T+LGRPWK YSRTV+MQ++I   + P GW 
Sbjct: 498 DPNQNTGISIINCTIKAAPDLAAEPKSA-MTFLGRPWKPYSRTVFMQSYISDIVQPVGWL 557

Query: 684 PWSSEY-LSTLYYAEYNNTGAGSNTKNRVTWPGYHVIYNATDASNFTVSNFLVGDVWLPP 733
            W+    L T+YY EY+N G G+NT  RV W GY+++ N  +A NFTV NF +GD WLP 
Sbjct: 558 EWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLL-NLAEAMNFTVYNFTMGDTWLPQ 616

BLAST of Lag0037412 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 1.0e-123
Identity = 251/549 (45.72%), Postives = 335/549 (61.02%), Query Frame = 0

Query: 205 PAKAICKFTSNPSYC---ISVLQKSTGNVYESGRFSMRRSLSKASRFFDLIEKHLQNSST 264
           P    CK T  P  C   +S ++ S  + Y  G+F+M++ L +A R   +I +  Q    
Sbjct: 83  PPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQRVEA 142

Query: 265 LPKSVT----GALTDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTNI 324
            P + T     A+ DC  LA+L++ +L      +   +  +T +  D + SLL  ++TN 
Sbjct: 143 DPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAE-LMTAALVDRVTSLLGGVVTNQ 202

Query: 325 DTCLDGLKTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTLE-HPGRKHL 384
            TCLDGL    SG +    +  PL + T+ YS+SL     G V    NR L+ + G K  
Sbjct: 203 QTCLDGLVDAKSGFATA--IGTPLGNLTRLYSVSL-----GLVSHALNRNLKRYKGSKGK 262

Query: 385 QFRKGPLPLR----------MSRHDRAVYNSVANRR--RLTSSSDDGVLINGIVVVSQDG 444
            F  G  P+R              D+      ANR    L  +S   +L+   V V    
Sbjct: 263 IFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYE 322

Query: 445 TGDFLTITDAVNASPNNSLAANGYFLIYITAGVYQEYVSIPSKKKYLLMIGDGINQTIIT 504
           T +F TIT+AV A+PN++    GYF+IY  AG+Y+EYV I +KK+ +++IGDGIN+TII+
Sbjct: 323 TDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIIS 382

Query: 505 GNRSVVDGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAVRSGADMAVFYSCSFE 564
           GN S +DGWTT+NS+TFAV  + F+AV++TF+NTAG  K QAVAVR+ AD + FY CSFE
Sbjct: 383 GNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFE 442

Query: 565 GYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQSNMITAQGRSD 624
           GYQDTLY HSLRQF+RECDIYGT+DFIFGNAA +FQNCNIY R P   Q N +TA GR+D
Sbjct: 443 GYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTD 502

Query: 625 PNQNTGTSIHNCTIRATSDLAASNSYINKTYLGRPWKQYSRTVYMQTFIDGFINPGGWDP 684
           PNQ TG SI NCTI A  DLAA +     T+LGRPWK YSRTVY+Q++I   + P GW  
Sbjct: 503 PNQKTGISIINCTIGAAPDLAA-DPKSTMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLE 562

Query: 685 WS-SEYLSTLYYAEYNNTGAGSNTKNRVTWPGYHVIYNATDASNFTVSNFLVGDVWLPPT 733
           W+ +  L T+ Y EY+N G G++T  RV W GY ++ N   A NFTV NF +GD WLP T
Sbjct: 563 WNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLL-NLVQAMNFTVYNFTLGDTWLPQT 621

BLAST of Lag0037412 vs. ExPASy TrEMBL
Match: A0A0A0LHM0 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G890080 PE=3 SV=1)

HSP 1 Score: 1022.3 bits (2642), Expect = 9.9e-295
Identity = 502/565 (88.85%), Postives = 536/565 (94.87%), Query Frame = 0

Query: 170 MACKFLFPPLVASFLILLTLLSSSSAADDSLTNFSPAKAICKFTSNPSYCISVLQKS-TG 229
           MACKFLFPPL+ASFLILLTL +SS A+DDSLTNF P KAICK TSNPSYCI+VL++S  G
Sbjct: 1   MACKFLFPPLLASFLILLTLFTSSFASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDG 60

Query: 230 NVYESGRFSMRRSLSKASRFFDLIEKHLQNSSTLPKSVTGALTDCQYLAQLNINFLSNSF 289
           N+Y+SGRFS+RRSLSKA+RF DLIEKHLQNSSTLP S+ GAL DCQYLAQLN+NFLSNSF
Sbjct: 61  NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF 120

Query: 290 RAVNGTDKKLTYSKADYIQSLLSAILTNIDTCLDGLKTVASGSSLEKDLLAPLIDCTKSY 349
           RAVNGTD+KLTYSKADYIQSLLSAILTNIDTCLDGL TVASGSSLEKDLLAPLIDCTKSY
Sbjct: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180

Query: 350 SLSLDLFTKGWVP-RNRNRTLEHPGRKHLQFRKGPLPLRMSRHDRAVYNSVANRRRLTSS 409
           SLSLDLFTKGWVP RNRNRTLEHPG+KHLQFRKGPLPLRMSRHDRAVYNSVANRR+L+SS
Sbjct: 181 SLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS 240

Query: 410 SDDGVLINGIVVVSQDGTGDFLTITDAVNASPNNSLAANGYFLIYITAGVYQEYVSIPSK 469
           SDDGVL+NG+VVVSQDG GDFL ITDA+NA+PNNSLA++GYFLIYITAGVYQEYVS+PSK
Sbjct: 241 SDDGVLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVSVPSK 300

Query: 470 KKYLLMIGDGINQTIITGNRSVVDGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAV 529
           KKYLLMIGDGINQTIITGNRSV DGWTTFNSATFAVAAEGFMAVNIT QNTAGAIKGQAV
Sbjct: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAV 360

Query: 530 AVRSGADMAVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 589
           A+RSGADM VFYSCSFEG+QDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR
Sbjct: 361 ALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420

Query: 590 LPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNSYINKTYLGRPWKQYSRTV 649
           LPRPGQ+NMITAQGRSDPNQNTGTSIHNCTIRAT +LAAS+SY+NKTYLGRPWKQYSRTV
Sbjct: 421 LPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQYSRTV 480

Query: 650 YMQTFIDGFINPGGWDPWSSEYLSTLYYAEYNNTGAGSNTKNRVTWPGYHVIYNATDASN 709
           YMQTFIDGF+NP GWDPW+ EYLSTLYY EYNNTG GS+TKNRVTW GYHVI N TDA+N
Sbjct: 481 YMQTFIDGFVNPKGWDPWTGEYLSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNVTDAAN 540

Query: 710 FTVSNFLVGDVWLPPTDVPYTGGFV 733
           FT+SNFLVGD WLPPT VPY GGFV
Sbjct: 541 FTISNFLVGDAWLPPTWVPYMGGFV 565

BLAST of Lag0037412 vs. ExPASy TrEMBL
Match: A0A5A7TAP2 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005740 PE=3 SV=1)

HSP 1 Score: 1021.1 bits (2639), Expect = 2.2e-294
Identity = 506/565 (89.56%), Postives = 534/565 (94.51%), Query Frame = 0

Query: 170 MACKFLFPPLVASFLILLTLLSSSSAADDSLTNFSPAKAICKFTSNPSYCISVLQKS-TG 229
           MACKFLFPPL   FLILLTL +SS A+DDSLTN  P KAICK TSNPSYCI+VL++S  G
Sbjct: 1   MACKFLFPPL---FLILLTLFTSSFASDDSLTNIFPTKAICKLTSNPSYCITVLKQSRDG 60

Query: 230 NVYESGRFSMRRSLSKASRFFDLIEKHLQNSSTLPKSVTGALTDCQYLAQLNINFLSNSF 289
           N+Y+SGRFS+RRSLSKASRF DLIEKHLQNSSTLPKS+ GAL DCQYLAQLN+NFLSNSF
Sbjct: 61  NIYDSGRFSIRRSLSKASRFLDLIEKHLQNSSTLPKSIIGALKDCQYLAQLNMNFLSNSF 120

Query: 290 RAVNGTDKKLTYSKADYIQSLLSAILTNIDTCLDGLKTVASGSSLEKDLLAPLIDCTKSY 349
           RAVNGTD+KLTYSKADYIQSLLSAILTNIDTCLDGL TVASGSSLEKDLLAPLIDCTKSY
Sbjct: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180

Query: 350 SLSLDLFTKGWVPR-NRNRTLEHPGRKHLQFRKGPLPLRMSRHDRAVYNSVANRRRLTSS 409
           SLSLDLFTKGWVPR NRNRTLEHPG+KHLQFRKGPLPLRMSRHDRAVYNSVANRR+L+SS
Sbjct: 181 SLSLDLFTKGWVPRWNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS 240

Query: 410 SDDGVLINGIVVVSQDGTGDFLTITDAVNASPNNSLAANGYFLIYITAGVYQEYVSIPSK 469
           SDDGVL+NGI VVSQDG GDFL ITDA+NA+PNNSLAANGYFLIYITAGVYQEYVS+PSK
Sbjct: 241 SDDGVLVNGIAVVSQDGEGDFLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSK 300

Query: 470 KKYLLMIGDGINQTIITGNRSVVDGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAV 529
           KKYLLMIGDGINQTIITGNRSV DGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAV
Sbjct: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAV 360

Query: 530 AVRSGADMAVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 589
           A+RSGADM VFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR
Sbjct: 361 ALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420

Query: 590 LPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNSYINKTYLGRPWKQYSRTV 649
           LP P Q+NMITAQGRSDPNQNTGTSIHNCTI+AT DLAAS+S++NKTYLGRPWKQYSRTV
Sbjct: 421 LPGPRQANMITAQGRSDPNQNTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTV 480

Query: 650 YMQTFIDGFINPGGWDPWSSEYLSTLYYAEYNNTGAGSNTKNRVTWPGYHVIYNATDASN 709
           YMQTFIDGF+NP GWDPWS EYLSTLYY EYNNTG GS+TKNRV WPGYHVI NATDA+N
Sbjct: 481 YMQTFIDGFVNPKGWDPWSGEYLSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAAN 540

Query: 710 FTVSNFLVGDVWLPPTDVPYTGGFV 733
           FT+SNFLVGDVWLPPT VPYTGGFV
Sbjct: 541 FTISNFLVGDVWLPPTGVPYTGGFV 562

BLAST of Lag0037412 vs. ExPASy TrEMBL
Match: A0A1S4E6G9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503665 PE=3 SV=1)

HSP 1 Score: 1021.1 bits (2639), Expect = 2.2e-294
Identity = 506/565 (89.56%), Postives = 534/565 (94.51%), Query Frame = 0

Query: 170 MACKFLFPPLVASFLILLTLLSSSSAADDSLTNFSPAKAICKFTSNPSYCISVLQKS-TG 229
           MACKFLFPPL   FLILLTL +SS A+DDSLTN  P KAICK TSNPSYCI+VL++S  G
Sbjct: 1   MACKFLFPPL---FLILLTLFTSSFASDDSLTNIFPTKAICKLTSNPSYCITVLKQSRDG 60

Query: 230 NVYESGRFSMRRSLSKASRFFDLIEKHLQNSSTLPKSVTGALTDCQYLAQLNINFLSNSF 289
           N+Y+SGRFS+RRSLSKASRF DLIEKHLQNSSTLPKS+ GAL DCQYLAQLN+NFLSNSF
Sbjct: 61  NIYDSGRFSIRRSLSKASRFLDLIEKHLQNSSTLPKSIIGALKDCQYLAQLNMNFLSNSF 120

Query: 290 RAVNGTDKKLTYSKADYIQSLLSAILTNIDTCLDGLKTVASGSSLEKDLLAPLIDCTKSY 349
           RAVNGTD+KLTYSKADYIQSLLSAILTNIDTCLDGL TVASGSSLEKDLLAPLIDCTKSY
Sbjct: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180

Query: 350 SLSLDLFTKGWVPR-NRNRTLEHPGRKHLQFRKGPLPLRMSRHDRAVYNSVANRRRLTSS 409
           SLSLDLFTKGWVPR NRNRTLEHPG+KHLQFRKGPLPLRMSRHDRAVYNSVANRR+L+SS
Sbjct: 181 SLSLDLFTKGWVPRWNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS 240

Query: 410 SDDGVLINGIVVVSQDGTGDFLTITDAVNASPNNSLAANGYFLIYITAGVYQEYVSIPSK 469
           SDDGVL+NGI VVSQDG GDFL ITDA+NA+PNNSLAANGYFLIYITAGVYQEYVS+PSK
Sbjct: 241 SDDGVLVNGIAVVSQDGEGDFLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSK 300

Query: 470 KKYLLMIGDGINQTIITGNRSVVDGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAV 529
           KKYLLMIGDGINQTIITGNRSV DGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAV
Sbjct: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAV 360

Query: 530 AVRSGADMAVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 589
           A+RSGADM VFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR
Sbjct: 361 ALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420

Query: 590 LPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNSYINKTYLGRPWKQYSRTV 649
           LP P Q+NMITAQGRSDPNQNTGTSIHNCTI+AT DLAAS+S++NKTYLGRPWKQYSRTV
Sbjct: 421 LPGPRQANMITAQGRSDPNQNTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTV 480

Query: 650 YMQTFIDGFINPGGWDPWSSEYLSTLYYAEYNNTGAGSNTKNRVTWPGYHVIYNATDASN 709
           YMQTFIDGF+NP GWDPWS EYLSTLYY EYNNTG GS+TKNRV WPGYHVI NATDA+N
Sbjct: 481 YMQTFIDGFVNPKGWDPWSGEYLSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAAN 540

Query: 710 FTVSNFLVGDVWLPPTDVPYTGGFV 733
           FT+SNFLVGDVWLPPT VPYTGGFV
Sbjct: 541 FTISNFLVGDVWLPPTGVPYTGGFV 562

BLAST of Lag0037412 vs. ExPASy TrEMBL
Match: A0A6J1J323 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111480894 PE=3 SV=1)

HSP 1 Score: 1002.3 bits (2590), Expect = 1.1e-288
Identity = 491/536 (91.60%), Postives = 513/536 (95.71%), Query Frame = 0

Query: 197 DDSLTNFSPAKAICKFTSNPSYCISVLQKSTGNVYESGRFSMRRSLSKASRFFDLIEKHL 256
           D SL +   AKAICKFTSNPSYCISVLQKS+GNVYESGRFSMRRSL+KA+ F +LIEKHL
Sbjct: 8   DGSLNDLFLAKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLAKATTFLNLIEKHL 67

Query: 257 QNSSTLPKSVTGALTDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTN 316
           QNSSTLPKSVTG L DCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTN
Sbjct: 68  QNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTN 127

Query: 317 IDTCLDGLKTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTLEHPGRKHL 376
           IDTCLDGL TVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRT +HP  KHL
Sbjct: 128 IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRDHPVTKHL 187

Query: 377 QFRKGPLPLRMSRHDRAVYNSVANRRRLTSSSDDGVLINGIVVVSQDGTGDFLTITDAVN 436
           +FRKGPLPLRMS+HDRAVYNSVANRRRL+SSSDDG+L+NGIVVVSQDGTG+FLTIT+AVN
Sbjct: 188 RFRKGPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFLTITEAVN 247

Query: 437 ASPNNSLAANGYFLIYITAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNRSVVDGWTTF 496
           A+PNNSLA NGYFLIYI AGVYQEYVSIPSKKKYLLMIGDGINQTIITGN+SV DGWTTF
Sbjct: 248 AAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSVADGWTTF 307

Query: 497 NSATFAVAAEGFMAVNITFQNTAGAIKGQAVAVRSGADMAVFYSCSFEGYQDTLYTHSLR 556
           NSATFAVAAEGFMAVNITFQNTAGAIKGQAVA+RSGADM++FYSCSFEGYQDTLYTHSLR
Sbjct: 308 NSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDTLYTHSLR 367

Query: 557 QFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQSNMITAQGRSDPNQNTGTSIHNC 616
           QFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPR GQ+NMITAQGRSDPNQNTGTSIHNC
Sbjct: 368 QFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNTGTSIHNC 427

Query: 617 TIRATSDLAASNSYINKTYLGRPWKQYSRTVYMQTFIDGFINPGGWDPWSSEYLSTLYYA 676
           TIRATSDLAAS SY+NKTYLGRPWKQYSRTVYMQTFIDGF+NP GWDPWS EYLSTLYYA
Sbjct: 428 TIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPAGWDPWSGEYLSTLYYA 487

Query: 677 EYNNTGAGSNTKNRVTWPGYHVIYNATDASNFTVSNFLVGDVWLPPTDVPYTGGFV 733
           EYNNTGAGSNTKNRVTWPGYHVI NATDASNFTVSNFLVGD WLPPT VPYTGGF+
Sbjct: 488 EYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTGGFI 543

BLAST of Lag0037412 vs. ExPASy TrEMBL
Match: A0A6J1FJR9 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111446371 PE=3 SV=1)

HSP 1 Score: 1001.9 bits (2589), Expect = 1.4e-288
Identity = 492/536 (91.79%), Postives = 512/536 (95.52%), Query Frame = 0

Query: 197 DDSLTNFSPAKAICKFTSNPSYCISVLQKSTGNVYESGRFSMRRSLSKASRFFDLIEKHL 256
           D SL +   AKAICKFTSNPSYCISVLQKS+GNVYESGRFSMRRSLSKAS F +LIEKHL
Sbjct: 8   DGSLNDLFAAKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLSKASAFLNLIEKHL 67

Query: 257 QNSSTLPKSVTGALTDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTN 316
           QNSSTLPKSVTG L DCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTN
Sbjct: 68  QNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTN 127

Query: 317 IDTCLDGLKTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTLEHPGRKHL 376
           IDTCLDGL TVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRT +HP  KHL
Sbjct: 128 IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRDHPVTKHL 187

Query: 377 QFRKGPLPLRMSRHDRAVYNSVANRRRLTSSSDDGVLINGIVVVSQDGTGDFLTITDAVN 436
           +FRKGPLPLRMS+HDRAVYNSVANRRRL SSSDDG+L+NGIVVVSQDGTG+FLTIT+AVN
Sbjct: 188 RFRKGPLPLRMSKHDRAVYNSVANRRRLLSSSDDGILVNGIVVVSQDGTGNFLTITEAVN 247

Query: 437 ASPNNSLAANGYFLIYITAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNRSVVDGWTTF 496
           A+PNNSLA NGYFLIYI AGVYQEYVSIPSKKKYLLMIGDGINQTIITGN+SV DGWTTF
Sbjct: 248 AAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSVADGWTTF 307

Query: 497 NSATFAVAAEGFMAVNITFQNTAGAIKGQAVAVRSGADMAVFYSCSFEGYQDTLYTHSLR 556
           NSATFAVAAEGFMAVNITFQNTAGAIKGQAVA+RSGADM++FYSCSFEGYQDTLYTHSLR
Sbjct: 308 NSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDTLYTHSLR 367

Query: 557 QFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQSNMITAQGRSDPNQNTGTSIHNC 616
           QFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPR GQ+NMITAQGRSDPNQNTGTSIHNC
Sbjct: 368 QFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNTGTSIHNC 427

Query: 617 TIRATSDLAASNSYINKTYLGRPWKQYSRTVYMQTFIDGFINPGGWDPWSSEYLSTLYYA 676
           TIRATSDLAAS SY+NKTYLGRPWKQYSRTVYMQTFIDGFINP GWDPWS EYLSTLYYA
Sbjct: 428 TIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFINPAGWDPWSGEYLSTLYYA 487

Query: 677 EYNNTGAGSNTKNRVTWPGYHVIYNATDASNFTVSNFLVGDVWLPPTDVPYTGGFV 733
           EYNN+GAGSNTKNRVTWPGYHVI NAT+ASNFTVSNFLVGD WLPPT VPYTGGF+
Sbjct: 488 EYNNSGAGSNTKNRVTWPGYHVISNATEASNFTVSNFLVGDAWLPPTGVPYTGGFI 543

BLAST of Lag0037412 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 642.5 bits (1656), Expect = 4.1e-184
Identity = 332/561 (59.18%), Postives = 412/561 (73.44%), Query Frame = 0

Query: 183 FLILLTLLSS-----SSAADDSLTNFSPAKAICKFTSNPSYCISVLQKSTGNVYESGRFS 242
           FL  L  LSS      SA   ++ N +    IC+F  +PSYC SVL    G++Y  GR S
Sbjct: 7   FLFTLACLSSLPSPFISAQIPAIGNATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLS 66

Query: 243 MRRSLSKASRFFDLIEKHL-QNSSTLPKSVTGALTDCQYLAQLNINFLSNSFRAVNGTDK 302
           +RRSLS+A RF  +I+  L +      KS  GAL DC++LA L +++L +S +  + T K
Sbjct: 67  LRRSLSRARRFISMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADST-K 126

Query: 303 KLTYSKADYIQSLLSAILTNIDTCLDGLKTVASGSSLEKDLLAPLIDCTKSYSLSLDLFT 362
            L+ S+A+ + + LSA +TN  TCL+GLK+ AS + L  D    L + TK Y +SL LF+
Sbjct: 127 TLSLSRAEDVHTFLSAAITNEQTCLEGLKSTASENGLSGD----LFNDTKLYGVSLALFS 186

Query: 363 KGWVP-RNRNRTLEHPG---RKHLQFRKGPLPLRMSRHDRAVYNSVANRRRLTSSSDDGV 422
           KGWVP R R+R +  P    +K   FR G LPL+M+   RAVYN+V  RR+L  S  D V
Sbjct: 187 KGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTV-TRRKLLQSDADAV 246

Query: 423 LINGIVVVSQDGTGDFLTITDAVNASPNNSLAANGYFLIYITAGVYQEYVSIPSKKKYLL 482
            ++ IV V Q+GTG+F TI  A+ A+PN +  +NGYFLIY+TAG+Y+EYV +P  K+Y++
Sbjct: 247 QVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVM 306

Query: 483 MIGDGINQTIITGNRSVVDGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAVRSG 542
           MIGDGINQT+ITGNRSVVDGWTTFNSATF ++   F+ VNIT +NTAG  KGQAVA+RSG
Sbjct: 307 MIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSG 366

Query: 543 ADMAVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPG 602
            D++VFYSCSFE YQDTLYTHSLRQF+RECD+YGTVDFIFGNAAVV QNCN+YPR PR G
Sbjct: 367 GDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKG 426

Query: 603 QSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNSYINKTYLGRPWKQYSRTVYMQTF 662
           QSN +TAQGR+DPNQNTGT+IH CTIR   DLA SN Y  KTYLGRPWK+YSRTV MQT+
Sbjct: 427 QSNEVTAQGRTDPNQNTGTAIHGCTIRPADDLATSN-YTVKTYLGRPWKEYSRTVVMQTY 486

Query: 663 IDGFINPGGWDPWSSEY-LSTLYYAEYNNTGAGSNTKNRVTWPGYHVIYNATDASNFTVS 722
           IDGF+ P GW+ WS ++ LSTLYYAEYNNTG GS+T NRVTWPGYHVI NATDASNFTV+
Sbjct: 487 IDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVI-NATDASNFTVT 546

Query: 723 NFLVGDVWLPPTDVPYTGGFV 733
           NFLVG+ W+  T VP+ GG +
Sbjct: 547 NFLVGEGWIGQTGVPFVGGLI 559

BLAST of Lag0037412 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 622.5 bits (1604), Expect = 4.4e-178
Identity = 326/571 (57.09%), Postives = 418/571 (73.20%), Query Frame = 0

Query: 179 LVASFLILLTLLSSSSAADDSLTNFSPAKAICKFTSNPSYCISVL-QKSTGNVYESGRFS 238
           LV  FL L TL      A  +L   + +  ICK T +P +C SV  Q S G+V E GRFS
Sbjct: 8   LVTLFLSLQTLF----IASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFS 67

Query: 239 MRRSLSKASRFFDLIEKHL-QNSSTLPKSVTGALTDCQYLAQLNINFLSNSFRAVNGT-- 298
           +R+SL+++ +F   I+++L +N++ L +S  GAL DC+YLA L  ++L  SF  VN T  
Sbjct: 68  LRKSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTS 127

Query: 299 DKKLTYSKADYIQSLLSAILTNIDTCLDGLKTVASGS-SLEKDLLAPLIDCTKSYSLSLD 358
            K L++SKAD IQ+LLSA LTN  TCLDG+ T AS S ++   +  PLI+ TK +S+SL 
Sbjct: 128 SKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLA 187

Query: 359 LFTKGWVPRNRNRTLE----HPGRKH------LQFRKGPLPLRMSRHDRAVYNSVANRRR 418
           LFTKGWVP+ + +       HP   H        FR G LPL+M+ H RAVY S++ R+ 
Sbjct: 188 LFTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKL 247

Query: 419 LTSSSD-DGVLINGIVVVSQDGTGDFLTITDAVNASPNNSLAANGYFLIYITAGVYQEYV 478
               +D + VL++ IV V+Q+GTG+F TIT+AVN++PN +    GYF+IY+T+GVY+E V
Sbjct: 248 ADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENV 307

Query: 479 SIPSKKKYLLMIGDGINQTIITGNRSVVDGWTTFNSATFAVAAEGFMAVNITFQNTAGAI 538
            I   K+YL+MIGDGIN+T++TGNR+VVDGWTTFNSATFAV +  F+AVN+TF+NTAG  
Sbjct: 308 VIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPE 367

Query: 539 KGQAVAVRSGADMAVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNC 598
           K QAVA+RS AD+++FYSCSFE YQDTLYTHSLRQF+RECDIYGTVDFIFGNAAVVFQ+C
Sbjct: 368 KHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDC 427

Query: 599 NIYPRLPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNSYINKTYLGRPWKQ 658
           N+YPR P   Q N ITAQGR+DPNQNTG SIHNCTI+   DL +SN Y  KTYLGRPWK+
Sbjct: 428 NLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSN-YTVKTYLGRPWKE 487

Query: 659 YSRTVYMQTFIDGFINPGGWDPWSSEY-LSTLYYAEYNNTGAGSNTKNRVTWPGYHVIYN 718
           YSRTV+MQ++ID  + P GW  W+ ++ LSTLYYAEYNNTG+GS+T +RV WPGYHVI N
Sbjct: 488 YSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVI-N 547

Query: 719 ATDASNFTVSNFLVGDVWLPPTDVPYTGGFV 733
           +TDA+NFTV NFL+GD W+  + VPY  G +
Sbjct: 548 STDANNFTVENFLLGDGWMVQSGVPYISGLL 572

BLAST of Lag0037412 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 621.3 bits (1601), Expect = 9.8e-178
Identity = 334/580 (57.59%), Postives = 416/580 (71.72%), Query Frame = 0

Query: 185 ILLTL---LSSSSAADDSLTNFSPAKAICKFTSNPSYCISVLQKSTGNVYESGRFSMRRS 244
           IL+TL   L S  A+  +L+N S    ICK T +P YC SV   S GNV + G FS+R+S
Sbjct: 7   ILITLSFFLQSVLASSQTLSNSS---TICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKS 66

Query: 245 LSKASRFFDLIEKHLQNSSTLPK-SVTGALTDCQYLAQLNINFLSNSFRAVNGTD----- 304
           LS++ +F   ++++++ ++ L + +V  AL DC++LA L +++L  SF  VN T      
Sbjct: 67  LSQSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSF 126

Query: 305 KKLTYSKADYIQSLLSAILTNIDTCLDGLKTVASGS---SLEKDLLAPLIDCTKSYSLSL 364
           K L++ KAD IQ+LLSA LTN  TCL+GL T AS S   ++   +  PL++ TK   +SL
Sbjct: 127 KPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSL 186

Query: 365 DLFTKGWVPRNRNR-------------TLEHPGRKHLQFRKGPLPLRMSRHDRAVYNSVA 424
            LFTKGWVP+ + R             T   P R    FR G LPL+M+   +AVY S++
Sbjct: 187 ALFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFR---LFRNGALPLKMTEKTKAVYESLS 246

Query: 425 NRRRLTSSS----DDG--VLINGIVVVSQDGTGDFLTITDAVNASPNNSLAANGYFLIYI 484
            R+     S    DDG  VLI+ IV VSQDGTG+F  IT AV A+PNN+  + G+FLIY+
Sbjct: 247 RRKLADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYV 306

Query: 485 TAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNRSVVDGWTTFNSATFAVAAEGFMAVNI 544
           TAG+Y+EY+SI   K+Y++MIGDGINQT++TGNRSVVDGWTTFNSATFAV A  F+AVNI
Sbjct: 307 TAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNI 366

Query: 545 TFQNTAGAIKGQAVAVRSGADMAVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFG 604
           TF+NTAG  K QAVA+RSGAD ++FYSCSFE YQDTLYTHSLRQF+RECD+YGTVDFIFG
Sbjct: 367 TFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFG 426

Query: 605 NAAVVFQNCNIYPRLPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNSYINK 664
           NAAVVFQNCN+YPR P P Q N ITAQGRSDPNQNTGTSI NCTI+   DL +SN Y  K
Sbjct: 427 NAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSN-YTVK 486

Query: 665 TYLGRPWKQYSRTVYMQTFIDGFINPGGWDPWSSEY-LSTLYYAEYNNTGAGSNTKNRVT 724
           TYLGRPWK+YSRTVYMQ++IDGF+ P GW  W+ ++ LSTLYYAEYNNTG GSNT NRVT
Sbjct: 487 TYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVT 546

Query: 725 WPGYHVIYNATDASNFTVSNFLVGDVWLPPTDVPYTGGFV 733
           WPGYHVI N+TDA+NFTV+   +   W+  T VPYT G +
Sbjct: 547 WPGYHVI-NSTDAANFTVTGLFIEADWIWKTGVPYTSGLI 578

BLAST of Lag0037412 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 458.4 bits (1178), Expect = 1.1e-128
Identity = 251/550 (45.64%), Postives = 347/550 (63.09%), Query Frame = 0

Query: 204 SPAKAICKFTSNPSYCISVL---QKSTGNVYESGRFSMRRSLSKASRFFDLIEKHLQNSS 263
           SP+ A CK T  P  C ++L   + S  + Y  G+F++++ L +ASR   +I  + +   
Sbjct: 78  SPSLA-CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVE 137

Query: 264 TLPKSVT----GALTDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTN 323
           + P S T    GA+ DC  L++L++N+L      +  T + +T +  +++ SLLS ++TN
Sbjct: 138 SKPGSATAEEIGAVADCGELSELSVNYLETVTTELK-TAQVMTAALVEHVNSLLSGVVTN 197

Query: 324 IDTCLDGLKTVASGSSLEKDLLAPLIDCTKSYSLSLDLF-------------TKGWVPRN 383
             TCLDGL    SG +    + +P+ + T+ YS+SL L              +KG +   
Sbjct: 198 QQTCLDGLVEAKSGFAAA--IGSPMGNLTRLYSISLGLVSHALNRNLKRFKASKGKILGG 257

Query: 384 RNRTLEHPGRKHLQFRKGPLPLRMSRHDRAVYNSVANRRRLTSSSDDGVLINGIVVVSQD 443
            N T   P    ++  +     +   +D+    +  N   L  +S   +L++  V+V   
Sbjct: 258 GNSTYREPLETLIKGLR-----KTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPF 317

Query: 444 GTGDFLTITDAVNASPNNSLAANGYFLIYITAGVYQEYVSIPSKKKYLLMIGDGINQTII 503
            + +F TITDA+ A+PNN+   +GYF+IY   GVY+EY+ +P  KK L+++GDGIN+TII
Sbjct: 318 KSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTII 377

Query: 504 TGNRSVVDGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAVRSGADMAVFYSCSF 563
           TGN +VVDGWTT+N ++FAV  E FMAV++TF+NTAG  K QAVA+R+ A+ + FY CSF
Sbjct: 378 TGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSF 437

Query: 564 EGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQSNMITAQGRS 623
           EGYQDTLY HSLRQF+RECDIYGTVDFIFGNAA +FQNCNIY R P   Q N ITA GR 
Sbjct: 438 EGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRL 497

Query: 624 DPNQNTGTSIHNCTIRATSDLAASNSYINKTYLGRPWKQYSRTVYMQTFIDGFINPGGWD 683
           DPNQNTG SI NCTI+A  DLAA       T+LGRPWK YSRTV+MQ++I   + P GW 
Sbjct: 498 DPNQNTGISIINCTIKAAPDLAAEPKSA-MTFLGRPWKPYSRTVFMQSYISDIVQPVGWL 557

Query: 684 PWSSEY-LSTLYYAEYNNTGAGSNTKNRVTWPGYHVIYNATDASNFTVSNFLVGDVWLPP 733
            W+    L T+YY EY+N G G+NT  RV W GY+++ N  +A NFTV NF +GD WLP 
Sbjct: 558 EWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLL-NLAEAMNFTVYNFTMGDTWLPQ 616

BLAST of Lag0037412 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 445.7 bits (1145), Expect = 7.4e-125
Identity = 251/549 (45.72%), Postives = 335/549 (61.02%), Query Frame = 0

Query: 205 PAKAICKFTSNPSYC---ISVLQKSTGNVYESGRFSMRRSLSKASRFFDLIEKHLQNSST 264
           P    CK T  P  C   +S ++ S  + Y  G+F+M++ L +A R   +I +  Q    
Sbjct: 83  PPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQRVEA 142

Query: 265 LPKSVT----GALTDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTNI 324
            P + T     A+ DC  LA+L++ +L      +   +  +T +  D + SLL  ++TN 
Sbjct: 143 DPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAE-LMTAALVDRVTSLLGGVVTNQ 202

Query: 325 DTCLDGLKTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTLE-HPGRKHL 384
            TCLDGL    SG +    +  PL + T+ YS+SL     G V    NR L+ + G K  
Sbjct: 203 QTCLDGLVDAKSGFATA--IGTPLGNLTRLYSVSL-----GLVSHALNRNLKRYKGSKGK 262

Query: 385 QFRKGPLPLR----------MSRHDRAVYNSVANRR--RLTSSSDDGVLINGIVVVSQDG 444
            F  G  P+R              D+      ANR    L  +S   +L+   V V    
Sbjct: 263 IFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYE 322

Query: 445 TGDFLTITDAVNASPNNSLAANGYFLIYITAGVYQEYVSIPSKKKYLLMIGDGINQTIIT 504
           T +F TIT+AV A+PN++    GYF+IY  AG+Y+EYV I +KK+ +++IGDGIN+TII+
Sbjct: 323 TDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIIS 382

Query: 505 GNRSVVDGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAVRSGADMAVFYSCSFE 564
           GN S +DGWTT+NS+TFAV  + F+AV++TF+NTAG  K QAVAVR+ AD + FY CSFE
Sbjct: 383 GNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFE 442

Query: 565 GYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQSNMITAQGRSD 624
           GYQDTLY HSLRQF+RECDIYGT+DFIFGNAA +FQNCNIY R P   Q N +TA GR+D
Sbjct: 443 GYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTD 502

Query: 625 PNQNTGTSIHNCTIRATSDLAASNSYINKTYLGRPWKQYSRTVYMQTFIDGFINPGGWDP 684
           PNQ TG SI NCTI A  DLAA +     T+LGRPWK YSRTVY+Q++I   + P GW  
Sbjct: 503 PNQKTGISIINCTIGAAPDLAA-DPKSTMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLE 562

Query: 685 WS-SEYLSTLYYAEYNNTGAGSNTKNRVTWPGYHVIYNATDASNFTVSNFLVGDVWLPPT 733
           W+ +  L T+ Y EY+N G G++T  RV W GY ++ N   A NFTV NF +GD WLP T
Sbjct: 563 WNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLL-NLVQAMNFTVYNFTLGDTWLPQT 621

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038898430.17.3e-30091.15probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Benincasa hispid... [more]
XP_031738568.12.0e-29488.85probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis sativus]... [more]
XP_016903565.14.6e-29489.56PREDICTED: probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucum... [more]
XP_023524144.16.8e-29092.16probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucurbita pepo s... [more]
KAG6608276.12.0e-28991.98putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosp... [more]
Match NameE-valueIdentityDescription
O222565.8e-18359.18Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3... [more]
Q8RXK76.2e-17757.09Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3... [more]
Q9SRX41.4e-17657.59Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37... [more]
Q94CB11.5e-12745.64Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3... [more]
Q9FF771.0e-12345.72Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A0A0LHM09.9e-29588.85Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G890080 PE=3 SV=1[more]
A0A5A7TAP22.2e-29489.56Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G00574... [more]
A0A1S4E6G92.2e-29489.56Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503665 PE=3 SV=1[more]
A0A6J1J3231.1e-28891.60Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111480894 PE=3 SV=1[more]
A0A6J1FJR91.4e-28891.79Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111446371 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G47550.14.1e-18459.18Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02330.14.4e-17857.09Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G02810.19.8e-17857.59Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G10720.21.1e-12845.64Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G04970.17.4e-12545.72Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 201..354
e-value: 2.8E-16
score: 70.1
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 182..328
e-value: 2.2E-10
score: 38.1
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 207..334
e-value: 2.1E-15
score: 57.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 205..357
e-value: 1.8E-19
score: 72.2
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 207..357
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 418..717
e-value: 9.1E-134
score: 444.8
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 414..731
e-value: 8.6E-119
score: 398.6
NoneNo IPR availablePANTHERPTHR31707:SF188PECTINESTERASEcoord: 182..730
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 182..730
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 208..359
e-value: 5.55522E-29
score: 110.991
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 564..573
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 414..729

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0037412.1Lag0037412.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity