Lag0037223 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0037223
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCysteinyl-tRNA synthetase
Locationchr2: 4406540 .. 4410997 (-)
RNA-Seq ExpressionLag0037223
SyntenyLag0037223
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCCTCCTAAAGTTCTACAATCCATCAACCTTCACTCGATTCTCCCCAATTTCAGTCTCTCTGGCGATTCTCCGCAGAACTCACCGCCATTCTCGTTTTCCATTCTTCAAGTCTGCTAGATGTTTCGCTACTTCTCTGAGCTCACCACAGCACCCAGTTTGCGAGGAGAATCACAAAGAAATCGGCGGCGTTAGGCGTGAGAGTACCCCGTCGGAACTGTGGCTGCATAACACGATGAGTAGGAAGAAAGAGCTGTTCAAGCCCAAGGTCGAAGGCAAAGTGGGCATGTACGTGTGCGGCGTCACTGCCTATGATCTCAGCCATATCGGCCATGCGCGTGTTTATGTTACATTCGATGTTCTCTACAGGTACTTGCTCTTTCTAAGTTTTACCTTTAATTTCCCTTTTCCGATTAAAGGCTCGTGACTGCTCGGGGAACGGACTGGTAAAGCTAACTTCGGTGAATTTTTTGTTTGGAAATGATGAATTTGTGTTTGTTTCGCTTGTGTAATGATTTGCTATTCTGTGTTGAAATGTAAGCAGGTATTTGAGGCATTTGGGATACGAAGTTCTTTATGTTCGCAATTTCACCGATGTGGATGACAAAGTGAGTGAATTTGCTAAGATGGTGCCATTTATGTAACGAACTGTATTTTTCTATGTTTAAATTCGTTCCCCTTGCACATACATGACCTCTGCCTTTTTAGGAAGTGAGATTGATAATAACAAGAAGACTGGGAACGCGAATGGATTGAACTTCTTTTTCCCCTTCCATTACTTTTTGCTTGATTCTATGATTTGTTAGGGGCATCTTCTATAGTGAGTGGTTTTCCTTTTCTTTGCTCATAAAGAGAGAAAGGGAGTTGATTAAATAGATAGAGTGCTTGAATACTGCATTTGGTAGCTAAATTTGAGTGCCTAATGAAGTTTAATATTCAGGAAAAGTGTAGATGCAAATAATGATCAACGTAAGAAATTGATGGGGTTAAGTCTTAGATTTGTGCTAGTGAACGAAAAAGCATCAAGAGATTGGGTTATCCTCTTTTGTTTGTTTCATCGTTGCTATGAAATGACTCCTACAAATTAGTCGTTACAGAATTCCACCATCCTTTGGTAAGGAAGGCTAAATTTCTCTGCTTTGAGTATACCAAGGCCACTATTATCCCACAACAATGAAACATAATCCTATTTAACAAGGTGCACCCATCCGAAGCAACTTCTTCCCGGAGAATATGCTGAGATGGCTTATCCCACATATTAGCTATCTTGGGAGGGACTTTGACCACCAAGAGATAATAAGAGGGTAGATTTATCATGACTGGTTTTATCATTGTGATTTTATCGCTGGAACCCTGAACCCTTTTCCATTTATTGAATTTCAAGGAATGGTGTTATGATTTTTTCCCCATTTTCCCTTGTCTTCCCTCCATTAGATAAGCGTTTTCTTGTCAAATACAACCCAAAACAGTCTATCTTCCTAGTTGCTAAATCCTACATGTTTCGAAGATAGATTCCTGTTGTCACTTTTGTTCCATGTAGTCAATTGGAGAACTTTAGGCTTCTTTAATGCTTGATATTATAATGTTTCTTTTATCTTGGTACCAACTTTTTTCATAATGCTATGACAGGATCCTGAGTCTGCACTTTTCAAATTCTGTAGATAATTGCAAGAGCAAATGAGTTGGGGGAAGATCCGCTTAATTTAAGTAAACGTTACTGTGAAGAATTCCGTCAGGATATGGTGTATCTTCATTGCTTGCCTCCTTCAGTTGAGCCACGAGTTTCAGACCACATGCCCCAGATCGTTGATATGATAAAGCAGGTAAAATTTTCCTTGCTTTGTGGTTCTCATTTGACAGAAGTTATAAGGCAATCATATTGGCAAATGCATGTGCTCTTTAAGTAATACCTACCAAGAGATCATGTGTTTGAACTTTTGGGTGAGCTTAATAAGAAAAACCCTTGATTAGTAGGGTTGGGGCCCCCCAATACGGGGTTATTAAAAAAGAAGGCAATCATATTATCTTGAATTTTCAACATGTCTTAAAACTGTCTTTTGTTTCAATAAGCATATATTATTTCACTTGTTGACTTCTTTTATGCTCTGTGCGTGTGTTTGTCTTGTCTTAATTGTTGATTCTAGGAGGGGAGGTGTGCATGACTAGCCATTAATTGTTTCTCTTGTGTCTTGACAGATTCTTGATAATGGATATGCCTATAGTGTTGATGGTGATGTGTACTTTAATGTGGACAAATTTCCAGAATATGGACTATTGTCTGGGCGAAAATTAGAGGATAACCGAGCTGGAGAGCGGGTTGCTGTGGACTCCAGGAAGAAAAACCCTGCTGATTTTGCTTTGTGGAAGGTCAATCTGGTTTCCTTGTATTATTACCAAGTGTTGCATGCATAGCATCGAACTATGAGATTCTCTAAATGCAAGGGATGGATAAATATGAATTTGTTTCTCGAATTGAAGATAGATTAAGAAAAGCAAACAACCCTATCAAAAAATTTGAGCTTCATTTTAGGTACAGATTGTCAGATAAACACAGCACTTCAAAATGGTATGATAATTTGCCTTACTTATCTTTCACACATCTGTGTCCTGTTTTTGTATTATTATTGAAGTCCTATGGTATCATGCAGTCTGCAAAGGAGGGAGAGCCATTTTGGGAAAGTCCATGGGGTCCTGGAAGGCCTGGATGGCATATAGAGTGTAGTGCCATGAGTGCAACTTATCTTGGGTATTCTTTTGATATACATGGGGGAGGGATGGATCTTGTATTTCCCCACCATGAAAATGAAATTGCTCAGAGTTGTGCTGCTTGCAGAACGAGTAATATAAGCTACTGGGTACATAATGGTTTTGTGACTATTGACTCAGAGAAAATGTCTAAATCTCTTGGGAACTTTTTCACCATTCGGCAGGTAGACTTAAATTAAATCTTTCATTTAGACTTTCGCATTTTCCTTTTTCCTTGCATTCTTCTTCTTCGTTTTTAACATCATGGAAATCCATTGCAGGATCTGATTTTTACCGTTTCATTTTCTTACAAGAGTTGGTTTTCAATTTGATAACTATAAATTGCCAGGTGATAGATCTCTACCATCCACTGGCTTTGAGGCTTTTCTTGTTGGGGACACACTATCGTTCTCCAATCAACTACTCTGATTTCCTGCTTGAAAGCGCCTCAGATCGCATTTATTACATCTATCAGGTACTATAGTGAACCTTTTTTTTAAAAAATATAAATTTTAATTTAATTTCACTGAATTTGAATGGTTGCAGACTCTTGAGGACTGTAGAACTGTAATTAGCCAGCAAGATGAATCAAGTTTTAAGGGTTCCATTGCCCCAAGTTTGGCGGAAGAAATCAACAAATTCTACAATGTTTTCCTGACCTCAATGTCCGATGATATTCACACTCCTGTGGTTTTGGCAGCGATATCGGATCCTTTGAAAATTATCAATGATTTGTTACATACTCGTAAGGTATTAGATATAAGTGTAGCTTGATTCATTCTGATATAGTAAATGCTTAGCTAGCTGTATGAGTATCTATTCGCCTAGAAGACATGCTTATCTTAGGCTTCAATGCACCTGTGTTTCATTCAGTATTATCTAATGGATAAACGTTCTGGTAAGATAATCATCTGTTAATGATCTTATTAATTTGACTTAACTAAGCGATTGTTCGAGATTATGAGTTTAATATCAGACCTGAAAGTTAAAAAGATGCTTTTGCCTGAGTCAACGAAATAGTGTTAGAGAGTGAAGGATCCCGTTTATATACCCTTAGCTTTAAAAACCACCCCCCCATCTTTCACAATTCACATATATTGTTAACTTGATGCTGCTTTCACATATTTTTCTATTCTTTGTAACATATGTAATTCAAAATCCATACAGGGAAAGAAGCAGGAATCTCGAATGGAATCTCTCGCGGCTTTGGAGAAGATAATCGGAAGCGTGCTGTCCATTTTAGGACTGATGCCTGCAAGTTACTCTGAGGTAGAAGAAACAGATTGATGTCATTACCATAATCAAATCCTGGAACAATCTTGAGGACTTCTGTACTTATAAGACTTCCAACATATAAATAAATGTTATACACTGACTTGTGAACCTCACCCTTTAGAAACTGATAATGATGAACAAAATTTTGAATTTGGTTATTTTGAAACAAACTTACTAAATATTTTTGTTGAGACAGGCTCTGCAAGAGCTGAAGGAGAAGGCTTTAACGCGCGCAAAGATGACGAACGATCAAGTAGTCGAGAAAATAGAAGAAAGAAATGCAGCAAGAAAGAATAAGGAGTATGAAAAATCTGATGCAATCAGGACTGATTTAGCTGCAGTTGGAATTACTCTAATGGATGGCCCAAATGGGACAACTTGGAGACCAGCTATTCCTCTTGCACTTCAAGAGCAGCAGGCTGCTTCAACTTGA

mRNA sequence

ATGGCTTCCCTCCTAAAGTTCTACAATCCATCAACCTTCACTCGATTCTCCCCAATTTCAGTCTCTCTGGCGATTCTCCGCAGAACTCACCGCCATTCTCGTTTTCCATTCTTCAAGTCTGCTAGATGTTTCGCTACTTCTCTGAGCTCACCACAGCACCCAGTTTGCGAGGAGAATCACAAAGAAATCGGCGGCGTTAGGCGTGAGAGTACCCCGTCGGAACTGTGGCTGCATAACACGATGAGTAGGAAGAAAGAGCTGTTCAAGCCCAAGGTCGAAGGCAAAGTGGGCATGTACGTGTGCGGCGTCACTGCCTATGATCTCAGCCATATCGGCCATGCGCGTGTTTATGTTACATTCGATGTTCTCTACAGGTATTTGAGGCATTTGGGATACGAAGTTCTTTATGTTCGCAATTTCACCGATGTGGATGACAAAATAATTGCAAGAGCAAATGAGTTGGGGGAAGATCCGCTTAATTTAAGTAAACGTTACTGTGAAGAATTCCGTCAGGATATGGTGTATCTTCATTGCTTGCCTCCTTCAGTTGAGCCACGAGTTTCAGACCACATGCCCCAGATCGTTGATATGATAAAGCAGATTCTTGATAATGGATATGCCTATAGTGTTGATGGTGATGTGTACTTTAATGTGGACAAATTTCCAGAATATGGACTATTGTCTGGGCGAAAATTAGAGGATAACCGAGCTGGAGAGCGGGTTGCTGTGGACTCCAGGAAGAAAAACCCTGCTGATTTTGCTTTGTGGAAGTCTGCAAAGGAGGGAGAGCCATTTTGGGAAAGTCCATGGGGTCCTGGAAGGCCTGGATGGCATATAGAGTGTAGTGCCATGAGTGCAACTTATCTTGGGTATTCTTTTGATATACATGGGGGAGGGATGGATCTTGTATTTCCCCACCATGAAAATGAAATTGCTCAGAGTTGTGCTGCTTGCAGAACGAGTAATATAAGCTACTGGGTACATAATGGTTTTGTGACTATTGACTCAGAGAAAATGTCTAAATCTCTTGGGAACTTTTTCACCATTCGGCAGGTGATAGATCTCTACCATCCACTGGCTTTGAGGCTTTTCTTGTTGGGGACACACTATCGTTCTCCAATCAACTACTCTGATTTCCTGCTTGAAAGCGCCTCAGATCGCATTTATTACATCTATCAGACTCTTGAGGACTGTAGAACTGTAATTAGCCAGCAAGATGAATCAAGTTTTAAGGGTTCCATTGCCCCAAGTTTGGCGGAAGAAATCAACAAATTCTACAATGTTTTCCTGACCTCAATGTCCGATGATATTCACACTCCTGTGGTTTTGGCAGCGATATCGGATCCTTTGAAAATTATCAATGATTTGTTACATACTCGTAAGGGAAAGAAGCAGGAATCTCGAATGGAATCTCTCGCGGCTTTGGAGAAGATAATCGGAAGCGTGCTGTCCATTTTAGGACTGATGCCTGCAAGTTACTCTGAGGCTCTGCAAGAGCTGAAGGAGAAGGCTTTAACGCGCGCAAAGATGACGAACGATCAAGTAGTCGAGAAAATAGAAGAAAGAAATGCAGCAAGAAAGAATAAGGAGTATGAAAAATCTGATGCAATCAGGACTGATTTAGCTGCAGTTGGAATTACTCTAATGGATGGCCCAAATGGGACAACTTGGAGACCAGCTATTCCTCTTGCACTTCAAGAGCAGCAGGCTGCTTCAACTTGA

Coding sequence (CDS)

ATGGCTTCCCTCCTAAAGTTCTACAATCCATCAACCTTCACTCGATTCTCCCCAATTTCAGTCTCTCTGGCGATTCTCCGCAGAACTCACCGCCATTCTCGTTTTCCATTCTTCAAGTCTGCTAGATGTTTCGCTACTTCTCTGAGCTCACCACAGCACCCAGTTTGCGAGGAGAATCACAAAGAAATCGGCGGCGTTAGGCGTGAGAGTACCCCGTCGGAACTGTGGCTGCATAACACGATGAGTAGGAAGAAAGAGCTGTTCAAGCCCAAGGTCGAAGGCAAAGTGGGCATGTACGTGTGCGGCGTCACTGCCTATGATCTCAGCCATATCGGCCATGCGCGTGTTTATGTTACATTCGATGTTCTCTACAGGTATTTGAGGCATTTGGGATACGAAGTTCTTTATGTTCGCAATTTCACCGATGTGGATGACAAAATAATTGCAAGAGCAAATGAGTTGGGGGAAGATCCGCTTAATTTAAGTAAACGTTACTGTGAAGAATTCCGTCAGGATATGGTGTATCTTCATTGCTTGCCTCCTTCAGTTGAGCCACGAGTTTCAGACCACATGCCCCAGATCGTTGATATGATAAAGCAGATTCTTGATAATGGATATGCCTATAGTGTTGATGGTGATGTGTACTTTAATGTGGACAAATTTCCAGAATATGGACTATTGTCTGGGCGAAAATTAGAGGATAACCGAGCTGGAGAGCGGGTTGCTGTGGACTCCAGGAAGAAAAACCCTGCTGATTTTGCTTTGTGGAAGTCTGCAAAGGAGGGAGAGCCATTTTGGGAAAGTCCATGGGGTCCTGGAAGGCCTGGATGGCATATAGAGTGTAGTGCCATGAGTGCAACTTATCTTGGGTATTCTTTTGATATACATGGGGGAGGGATGGATCTTGTATTTCCCCACCATGAAAATGAAATTGCTCAGAGTTGTGCTGCTTGCAGAACGAGTAATATAAGCTACTGGGTACATAATGGTTTTGTGACTATTGACTCAGAGAAAATGTCTAAATCTCTTGGGAACTTTTTCACCATTCGGCAGGTGATAGATCTCTACCATCCACTGGCTTTGAGGCTTTTCTTGTTGGGGACACACTATCGTTCTCCAATCAACTACTCTGATTTCCTGCTTGAAAGCGCCTCAGATCGCATTTATTACATCTATCAGACTCTTGAGGACTGTAGAACTGTAATTAGCCAGCAAGATGAATCAAGTTTTAAGGGTTCCATTGCCCCAAGTTTGGCGGAAGAAATCAACAAATTCTACAATGTTTTCCTGACCTCAATGTCCGATGATATTCACACTCCTGTGGTTTTGGCAGCGATATCGGATCCTTTGAAAATTATCAATGATTTGTTACATACTCGTAAGGGAAAGAAGCAGGAATCTCGAATGGAATCTCTCGCGGCTTTGGAGAAGATAATCGGAAGCGTGCTGTCCATTTTAGGACTGATGCCTGCAAGTTACTCTGAGGCTCTGCAAGAGCTGAAGGAGAAGGCTTTAACGCGCGCAAAGATGACGAACGATCAAGTAGTCGAGAAAATAGAAGAAAGAAATGCAGCAAGAAAGAATAAGGAGTATGAAAAATCTGATGCAATCAGGACTGATTTAGCTGCAGTTGGAATTACTCTAATGGATGGCCCAAATGGGACAACTTGGAGACCAGCTATTCCTCTTGCACTTCAAGAGCAGCAGGCTGCTTCAACTTGA

Protein sequence

MASLLKFYNPSTFTRFSPISVSLAILRRTHRHSRFPFFKSARCFATSLSSPQHPVCEENHKEIGGVRRESTPSELWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVYVTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLNLSKRYCEEFRQDMVYLHCLPPSVEPRVSDHMPQIVDMIKQILDNGYAYSVDGDVYFNVDKFPEYGLLSGRKLEDNRAGERVAVDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHGGGMDLVFPHHENEIAQSCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYHPLALRLFLLGTHYRSPINYSDFLLESASDRIYYIYQTLEDCRTVISQQDESSFKGSIAPSLAEEINKFYNVFLTSMSDDIHTPVVLAAISDPLKIINDLLHTRKGKKQESRMESLAALEKIIGSVLSILGLMPASYSEALQELKEKALTRAKMTNDQVVEKIEERNAARKNKEYEKSDAIRTDLAAVGITLMDGPNGTTWRPAIPLALQEQQAAST
Homology
BLAST of Lag0037223 vs. NCBI nr
Match: XP_023524130.1 (cysteine--tRNA ligase, chloroplastic/mitochondrial-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1062.0 bits (2745), Expect = 1.8e-306
Identity = 527/574 (91.81%), Postives = 542/574 (94.43%), Query Frame = 0

Query: 1   MASLLKFYNPSTFTRFSPISVSLAI-LRRTHRHSRFPFFKSARCFATSLSSPQHPVCEEN 60
           MASLLK YNP   TRFSPISVS AI LRRT RH RFPFFKSARCF TSLS  QHPV EEN
Sbjct: 1   MASLLKLYNPLMVTRFSPISVSQAILLRRTRRHFRFPFFKSARCFTTSLSLSQHPVNEEN 60

Query: 61  HKEIGGVRRESTPSELWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVYVT 120
           HKEIGGV R+ T SELWLHNTMSRKKELFK KVEGKVGMYVCGVTAYDLSHIGHARVYVT
Sbjct: 61  HKEIGGVPRQCTSSELWLHNTMSRKKELFKTKVEGKVGMYVCGVTAYDLSHIGHARVYVT 120

Query: 121 FDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLNLSKRYCEEFRQDMVYLHCL 180
           FDVLYRYL+HLGYEVLYVRNFTDVDDKII RANELGEDPLNLSKRYCEEF QDMV LHCL
Sbjct: 121 FDVLYRYLKHLGYEVLYVRNFTDVDDKIIVRANELGEDPLNLSKRYCEEFCQDMVSLHCL 180

Query: 181 PPSVEPRVSDHMPQIVDMIKQILDNGYAYSVDGDVYFNVDKFPEYGLLSGRKLEDNRAGE 240
           PPSVEPRVSDHMPQI+DMIKQILDNGYAY VDGDVYFNVDKFPEYGLLSGRKLEDNRAGE
Sbjct: 181 PPSVEPRVSDHMPQIIDMIKQILDNGYAYCVDGDVYFNVDKFPEYGLLSGRKLEDNRAGE 240

Query: 241 RVAVDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHGGG 300
           RVAVDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHGGG
Sbjct: 241 RVAVDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHGGG 300

Query: 301 MDLVFPHHENEIAQSCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYHPL 360
           MDLVFPHHENEIAQSCA C+TSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYHPL
Sbjct: 301 MDLVFPHHENEIAQSCATCKTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYHPL 360

Query: 361 ALRLFLLGTHYRSPINYSDFLLESASDRIYYIYQTLEDCRTVISQQDESSFKGSIAPSLA 420
           ALRLFLLGTHYRSPINYSDFLLESASDRI+YIYQTL+DC+TV+SQQDESS KGSI PSL 
Sbjct: 361 ALRLFLLGTHYRSPINYSDFLLESASDRIFYIYQTLDDCKTVLSQQDESSLKGSIPPSLM 420

Query: 421 EEINKFYNVFLTSMSDDIHTPVVLAAISDPLKIINDLLHTRKGKKQESRMESLAALEKII 480
           EEINKFYNVFLTSMSDDIHT VVLAA+SDPLKIINDLLHTRKGKKQE R+ESLAALEKII
Sbjct: 421 EEINKFYNVFLTSMSDDIHTNVVLAALSDPLKIINDLLHTRKGKKQEFRIESLAALEKII 480

Query: 481 GSVLSILGLMPASYSEALQELKEKALTRAKMTNDQVVEKIEERNAARKNKEYEKSDAIRT 540
           G +LSILGLMPASYSEAL +LKEKALTRAKMTN+QVV+KIEERNAARKNKEYEKSDAIR 
Sbjct: 481 GRLLSILGLMPASYSEALGQLKEKALTRAKMTNEQVVQKIEERNAARKNKEYEKSDAIRN 540

Query: 541 DLAAVGITLMDGPNGTTWRPAIPLALQEQQAAST 574
           DLA VGITLMDGPNGTTWRP IPLALQE Q+AST
Sbjct: 541 DLAGVGITLMDGPNGTTWRPTIPLALQEHQSAST 574

BLAST of Lag0037223 vs. NCBI nr
Match: XP_038900128.1 (cysteine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 1057.0 bits (2732), Expect = 5.9e-305
Identity = 525/576 (91.15%), Postives = 544/576 (94.44%), Query Frame = 0

Query: 1   MASLLKFYNPSTFTRFSPISVSLAILRRTHRHSRFPFFKSAR---CFATSLSSPQHPVCE 60
           MASLLKFYNP TF RFSPIS+S AILRRTHR+S F FFKSAR   CF +S SS Q  V  
Sbjct: 1   MASLLKFYNPLTFARFSPISLSQAILRRTHRNSHFSFFKSARSFACFTSSPSSSQLLVSA 60

Query: 61  ENHKEIGGVRRESTPSELWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVY 120
           +  KEI  V  E+T  ELWLHNTMSRKKE+FKPKVEGKVGMYVCGVTAYDLSHIGHARVY
Sbjct: 61  QIPKEIAAVNHENTSLELWLHNTMSRKKEVFKPKVEGKVGMYVCGVTAYDLSHIGHARVY 120

Query: 121 VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLNLSKRYCEEFRQDMVYLH 180
           VTFDVLYRYLRHLG+EVLYVRNFTDVDDKIIARANELGEDPLNLS+RYCEEF QDMVYLH
Sbjct: 121 VTFDVLYRYLRHLGHEVLYVRNFTDVDDKIIARANELGEDPLNLSRRYCEEFCQDMVYLH 180

Query: 181 CLPPSVEPRVSDHMPQIVDMIKQILDNGYAYSVDGDVYFNVDKFPEYGLLSGRKLEDNRA 240
           CLPPSVEPRVSDHMPQI+DMIKQILDNGYAY VDGDVYFNVDKFPEYGLLSGRKLEDNRA
Sbjct: 181 CLPPSVEPRVSDHMPQIIDMIKQILDNGYAYCVDGDVYFNVDKFPEYGLLSGRKLEDNRA 240

Query: 241 GERVAVDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHG 300
           GERVAVD+RKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHG
Sbjct: 241 GERVAVDTRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHG 300

Query: 301 GGMDLVFPHHENEIAQSCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH 360
           GGMDLVFPHHENEIAQSCAACR+SN+SYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH
Sbjct: 301 GGMDLVFPHHENEIAQSCAACRSSNVSYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH 360

Query: 361 PLALRLFLLGTHYRSPINYSDFLLESASDRIYYIYQTLEDCRTVISQQDESSFKGSIAPS 420
           PLALRLFLLGTHYRSPINYSD LLESASDRI+YIYQTLEDCRTVISQQDESSFKGSIAPS
Sbjct: 361 PLALRLFLLGTHYRSPINYSDLLLESASDRIFYIYQTLEDCRTVISQQDESSFKGSIAPS 420

Query: 421 LAEEINKFYNVFLTSMSDDIHTPVVLAAISDPLKIINDLLHTRKGKKQESRMESLAALEK 480
           L EEINKFYNVFLTSMSDDIHTPVVLAA+SDPLKIINDLLHTRKGKKQE RMESLAALEK
Sbjct: 421 LVEEINKFYNVFLTSMSDDIHTPVVLAALSDPLKIINDLLHTRKGKKQEFRMESLAALEK 480

Query: 481 IIGSVLSILGLMPASYSEALQELKEKALTRAKMTNDQVVEKIEERNAARKNKEYEKSDAI 540
           IIG+VLSILGLMPASYSEALQ+LKEKALTRAKMTNDQVV+KIEERNAARKNKEYEKSDAI
Sbjct: 481 IIGNVLSILGLMPASYSEALQQLKEKALTRAKMTNDQVVQKIEERNAARKNKEYEKSDAI 540

Query: 541 RTDLAAVGITLMDGPNGTTWRPAIPLALQEQQAAST 574
           R DLAA GI LMDGPNGTTWRP +PLALQEQQA+ST
Sbjct: 541 RRDLAAFGIALMDGPNGTTWRPTVPLALQEQQASST 576

BLAST of Lag0037223 vs. NCBI nr
Match: KAG6591556.1 (Cysteine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1055.4 bits (2728), Expect = 1.7e-304
Identity = 520/572 (90.91%), Postives = 539/572 (94.23%), Query Frame = 0

Query: 1   MASLLKFYNPSTFTRFSPISVSLAILRRTHRHSRFPFFKSARCFATSLSSP---QHPVCE 60
           MASLLKFYNP +FTRFSP+SVS AI RR HRHS FPFFK ARCFA   SSP   Q PV  
Sbjct: 1   MASLLKFYNPLSFTRFSPVSVSQAIFRRAHRHSHFPFFKPARCFACFTSSPSSSQPPVSA 60

Query: 61  ENHKEIGGVRRESTPSELWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVY 120
           +NHKEIGGVR ES+ SELWLHNTMSRKKE+FKPKVEGKVGMYVCGVTAYDLSHIGHARVY
Sbjct: 61  QNHKEIGGVRHESSSSELWLHNTMSRKKEVFKPKVEGKVGMYVCGVTAYDLSHIGHARVY 120

Query: 121 VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLNLSKRYCEEFRQDMVYLH 180
           VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPL+LS+RYCEEF QDM YLH
Sbjct: 121 VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLDLSRRYCEEFCQDMRYLH 180

Query: 181 CLPPSVEPRVSDHMPQIVDMIKQILDNGYAYSVDGDVYFNVDKFPEYGLLSGRKLEDNRA 240
           CL PSVEPRVSDHM QI+DMIKQILDNGYAY VDGDVYFNVDKFPEYGLLSGRKLEDNRA
Sbjct: 181 CLSPSVEPRVSDHMSQIIDMIKQILDNGYAYRVDGDVYFNVDKFPEYGLLSGRKLEDNRA 240

Query: 241 GERVAVDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHG 300
           GERVAVDSRKKNPADFALWKSAKEGEPFW SPWGPGRPGWHIECSAMSA YLGYSFDIHG
Sbjct: 241 GERVAVDSRKKNPADFALWKSAKEGEPFWASPWGPGRPGWHIECSAMSAAYLGYSFDIHG 300

Query: 301 GGMDLVFPHHENEIAQSCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH 360
           GGMDLVFPHHENEIAQSCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH
Sbjct: 301 GGMDLVFPHHENEIAQSCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH 360

Query: 361 PLALRLFLLGTHYRSPINYSDFLLESASDRIYYIYQTLEDCRTVISQQDESSFKGSIAPS 420
           PLALRLFLLGTHYRSPINYSD LLESASDRI+YIYQTL+DCRTVISQQDESS K SI PS
Sbjct: 361 PLALRLFLLGTHYRSPINYSDLLLESASDRIFYIYQTLDDCRTVISQQDESSLKSSIPPS 420

Query: 421 LAEEINKFYNVFLTSMSDDIHTPVVLAAISDPLKIINDLLHTRKGKKQESRMESLAALEK 480
           L EEINKFYNVFLTSMSDDIHTPVVLAA+SDPLKIINDLLHTRKGKKQE RMESLAALEK
Sbjct: 421 LVEEINKFYNVFLTSMSDDIHTPVVLAALSDPLKIINDLLHTRKGKKQEFRMESLAALEK 480

Query: 481 IIGSVLSILGLMPASYSEALQELKEKALTRAKMTNDQVVEKIEERNAARKNKEYEKSDAI 540
           +IG+VLSILGLMPASYSEALQ+LKEKALTRAKMTN+QVV+KIEERNAARKNKEYEKSD I
Sbjct: 481 MIGNVLSILGLMPASYSEALQQLKEKALTRAKMTNEQVVQKIEERNAARKNKEYEKSDTI 540

Query: 541 RTDLAAVGITLMDGPNGTTWRPAIPLALQEQQ 570
           RTDLAAVGI LMDGPNGT+WRP +PLALQ+QQ
Sbjct: 541 RTDLAAVGIALMDGPNGTSWRPTVPLALQDQQ 572

BLAST of Lag0037223 vs. NCBI nr
Match: KAG7024444.1 (Cysteine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1053.1 bits (2722), Expect = 8.5e-304
Identity = 519/570 (91.05%), Postives = 537/570 (94.21%), Query Frame = 0

Query: 1   MASLLKFYNPSTFTRFSPISVSLAILRRTHRHSRFPFFKSARCFATSLSSP---QHPVCE 60
           MASLLKFYNP +FTRFSP+SVS AI RR HRHS FPFFK ARCFA   SSP   Q PV  
Sbjct: 1   MASLLKFYNPLSFTRFSPVSVSQAIFRRAHRHSHFPFFKPARCFACFTSSPSSSQPPVSA 60

Query: 61  ENHKEIGGVRRESTPSELWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVY 120
           +NHKEIGGVR ES+ SELWLHNTMSRKKE+FKPKVEGKVGMYVCGVTAYDLSHIGHARVY
Sbjct: 61  QNHKEIGGVRHESSSSELWLHNTMSRKKEVFKPKVEGKVGMYVCGVTAYDLSHIGHARVY 120

Query: 121 VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLNLSKRYCEEFRQDMVYLH 180
           VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPL+LS+RYCEEF QDM YLH
Sbjct: 121 VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLDLSRRYCEEFCQDMRYLH 180

Query: 181 CLPPSVEPRVSDHMPQIVDMIKQILDNGYAYSVDGDVYFNVDKFPEYGLLSGRKLEDNRA 240
           CL PSVEPRVSDHM QI+DMIKQILDNGYAY VDGDVYFNVDKFPEYGLLSGRKLEDNRA
Sbjct: 181 CLSPSVEPRVSDHMSQIIDMIKQILDNGYAYRVDGDVYFNVDKFPEYGLLSGRKLEDNRA 240

Query: 241 GERVAVDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHG 300
           GERVAVDSRKKNPADFALWKSAKEGEPFW SPWGPGRPGWHIECSAMSA YLGYSFDIHG
Sbjct: 241 GERVAVDSRKKNPADFALWKSAKEGEPFWASPWGPGRPGWHIECSAMSAAYLGYSFDIHG 300

Query: 301 GGMDLVFPHHENEIAQSCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH 360
           GGMDLVFPHHENEIAQSCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH
Sbjct: 301 GGMDLVFPHHENEIAQSCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH 360

Query: 361 PLALRLFLLGTHYRSPINYSDFLLESASDRIYYIYQTLEDCRTVISQQDESSFKGSIAPS 420
           PLALRLFLLGTHYRSPINYSD LLESASDRI+YIYQTL+DCRTVISQQDESS K SI PS
Sbjct: 361 PLALRLFLLGTHYRSPINYSDLLLESASDRIFYIYQTLDDCRTVISQQDESSLKSSIPPS 420

Query: 421 LAEEINKFYNVFLTSMSDDIHTPVVLAAISDPLKIINDLLHTRKGKKQESRMESLAALEK 480
           L EEINKFYNVFLTSMSDDIHTPVVLAA+SDPLKIINDLLHTRKGKKQE RMESLAALEK
Sbjct: 421 LVEEINKFYNVFLTSMSDDIHTPVVLAALSDPLKIINDLLHTRKGKKQEFRMESLAALEK 480

Query: 481 IIGSVLSILGLMPASYSEALQELKEKALTRAKMTNDQVVEKIEERNAARKNKEYEKSDAI 540
           +IG+VLSILGLMPASYSEALQ+LKEKALTRAKMTN+QVV+KIEERNAARKNKEYEKSD I
Sbjct: 481 MIGNVLSILGLMPASYSEALQQLKEKALTRAKMTNEQVVQKIEERNAARKNKEYEKSDTI 540

Query: 541 RTDLAAVGITLMDGPNGTTWRPAIPLALQE 568
           RTDLAAVGI LMDGPNGTTWRP +PLALQ+
Sbjct: 541 RTDLAAVGIALMDGPNGTTWRPTVPLALQD 570

BLAST of Lag0037223 vs. NCBI nr
Match: XP_008466439.1 (PREDICTED: cysteine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucumis melo] >TYK31452.1 cysteine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 1052.4 bits (2720), Expect = 1.4e-303
Identity = 520/576 (90.28%), Postives = 545/576 (94.62%), Query Frame = 0

Query: 1   MASLLKFYNPSTFTRFSPISVSLAILRRTHRHSRFPFFKSA---RCFATSLSSPQHPVCE 60
           MASLLKFYNP TFTRF+PISVS  ILRRT R+S F FF SA    CF +S +S Q PV  
Sbjct: 1   MASLLKFYNPLTFTRFTPISVSQVILRRTLRNSHFSFFNSATTFACFTSSPTSSQLPVSA 60

Query: 61  ENHKEIGGVRRESTPSELWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVY 120
            N K+I  VR EST SELWLHNTMSRKKE+FKPKVEGKVGMYVCGVTAYDLSHIGHARVY
Sbjct: 61  PNLKKINAVRHESTSSELWLHNTMSRKKEVFKPKVEGKVGMYVCGVTAYDLSHIGHARVY 120

Query: 121 VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLNLSKRYCEEFRQDMVYLH 180
           VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLNLS+RYCEEFR+DMVYLH
Sbjct: 121 VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLNLSRRYCEEFRRDMVYLH 180

Query: 181 CLPPSVEPRVSDHMPQIVDMIKQILDNGYAYSVDGDVYFNVDKFPEYGLLSGRKLEDNRA 240
           CLPPSVEP+VSDHMPQI+DMIKQILDNGYAY VDGDVYFNVDKFP+YG LSGRKLEDNRA
Sbjct: 181 CLPPSVEPQVSDHMPQIIDMIKQILDNGYAYCVDGDVYFNVDKFPDYGQLSGRKLEDNRA 240

Query: 241 GERVAVDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHG 300
           GERV+VDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSA+YLGYSFDIHG
Sbjct: 241 GERVSVDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSASYLGYSFDIHG 300

Query: 301 GGMDLVFPHHENEIAQSCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH 360
           GGMDLVFPHHENEIAQSCAACRTSN+SYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH
Sbjct: 301 GGMDLVFPHHENEIAQSCAACRTSNVSYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH 360

Query: 361 PLALRLFLLGTHYRSPINYSDFLLESASDRIYYIYQTLEDCRTVISQQDESSFKGSIAPS 420
           PLALRLFLLGTHYRSPINYSD LLESASDRI+YIYQTL+DCRTVISQQDESSFKGSIAPS
Sbjct: 361 PLALRLFLLGTHYRSPINYSDLLLESASDRIFYIYQTLDDCRTVISQQDESSFKGSIAPS 420

Query: 421 LAEEINKFYNVFLTSMSDDIHTPVVLAAISDPLKIINDLLHTRKGKKQESRMESLAALEK 480
           L EEINKFYNVFLTSMSDDIHTPVVLAA+SDPLKIINDLLHTRKGKKQE RMESLAALEK
Sbjct: 421 LMEEINKFYNVFLTSMSDDIHTPVVLAALSDPLKIINDLLHTRKGKKQEFRMESLAALEK 480

Query: 481 IIGSVLSILGLMPASYSEALQELKEKALTRAKMTNDQVVEKIEERNAARKNKEYEKSDAI 540
           IIG+VLSILGLMPASYSEALQ+LKEKAL RAKMTNDQV++KIEERNAARKNKEYEKSD+I
Sbjct: 481 IIGNVLSILGLMPASYSEALQQLKEKALKRAKMTNDQVLQKIEERNAARKNKEYEKSDSI 540

Query: 541 RTDLAAVGITLMDGPNGTTWRPAIPLALQEQQAAST 574
           RTDLAAVGI LMDGPNGTTWRP++PLALQE QA+ST
Sbjct: 541 RTDLAAVGIALMDGPNGTTWRPSVPLALQEHQASST 576

BLAST of Lag0037223 vs. ExPASy Swiss-Prot
Match: F4IPY2 (Cysteine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SYCO PE=2 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 3.9e-235
Identity = 408/567 (71.96%), Postives = 459/567 (80.95%), Query Frame = 0

Query: 3   SLLKFYNPSTFTRFSPISVSLAILRRTHRHSRFPFFKSARCFAT--SLSSPQHPVCEENH 62
           +L K   P T  RFS +  S   ++   R  +    +  RCF T  SL+    P+     
Sbjct: 7   NLFKSCRPFTPIRFSSLPKSQFRIQFPLRPGKETQLR--RCFTTLSSLTDGGAPI----- 66

Query: 63  KEIGGVRRESTPSELWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVYVTF 122
                    S   ELWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVYVTF
Sbjct: 67  ---------SGGKELWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVYVTF 126

Query: 123 DVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLNLSKRYCEEFRQDMVYLHCLP 182
           DVL RYL+HLGYEV YVRNFTDVDDKIIARA EL EDP++LS+R+CEEF +DM  L CL 
Sbjct: 127 DVLLRYLKHLGYEVSYVRNFTDVDDKIIARAKELEEDPISLSRRFCEEFNRDMEQLQCLD 186

Query: 183 PSVEPRVSDHMPQIVDMIKQILDNGYAYSVDGDVYFNVDKFPEYGLLSGRKLEDNRAGER 242
           PSV+PRVSDH+PQI+D+IKQILDNGYAY VDGD+YF+VDKFP YG LSGRKLEDNRAGER
Sbjct: 187 PSVQPRVSDHIPQIIDLIKQILDNGYAYKVDGDIYFSVDKFPTYGKLSGRKLEDNRAGER 246

Query: 243 VAVDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHGGGM 302
           VAVD+RKK+PADFALWK+AKEGEPFWESPWG GRPGWHIECSAMSA YLGYSFDIHGGGM
Sbjct: 247 VAVDTRKKHPADFALWKAAKEGEPFWESPWGRGRPGWHIECSAMSAAYLGYSFDIHGGGM 306

Query: 303 DLVFPHHENEIAQSCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYHPLA 362
           DLVFPHHENEIAQSCAAC +SNISYW+HNGFVT+DSEKMSKSLGNFFTIRQVIDLYHPLA
Sbjct: 307 DLVFPHHENEIAQSCAACDSSNISYWIHNGFVTVDSEKMSKSLGNFFTIRQVIDLYHPLA 366

Query: 363 LRLFLLGTHYRSPINYSDFLLESASDRIYYIYQTLEDCRTVISQQDESSFKGSIAPSLAE 422
           LRLFL+GTHYRSPINYSDFLLESAS+RI+YIYQTL DC + + ++D +   GS+      
Sbjct: 367 LRLFLMGTHYRSPINYSDFLLESASERIFYIYQTLHDCESALGEKDSTFENGSVPSDTLT 426

Query: 423 EINKFYNVFLTSMSDDIHTPVVLAAISDPLKIINDLLHTRKGKKQESRMESLAALEKIIG 482
            IN F   F+ SMSDD+ TPV LAA+S+PLK INDL+HTRKGKKQ  R ESL ALE  I 
Sbjct: 427 SINTFRTEFVASMSDDLLTPVTLAAMSEPLKTINDLIHTRKGKKQARREESLKALETTIR 486

Query: 483 SVLSILGLMPASYSEALQELKEKALTRAKMTNDQVVEKIEERNAARKNKEYEKSDAIRTD 542
            VL+ILGLMP SYSE L++LKEKAL RA +  + V+++++ER  ARKNKEYE+SDAIR D
Sbjct: 487 DVLTILGLMPTSYSEVLEQLKEKALKRAGLKEEDVLQRVQERTDARKNKEYERSDAIRKD 546

Query: 543 LAAVGITLMDGPNGTTWRPAIPLALQE 568
           LA VGI LMD P GTTWRPAIPLALQE
Sbjct: 547 LAKVGIALMDSPEGTTWRPAIPLALQE 557

BLAST of Lag0037223 vs. ExPASy Swiss-Prot
Match: Q0IZQ2 (Cysteine--tRNA ligase CPS1 homolog, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0556500 PE=2 SV=1)

HSP 1 Score: 725.3 bits (1871), Expect = 5.3e-208
Identity = 352/494 (71.26%), Postives = 419/494 (84.82%), Query Frame = 0

Query: 74  ELWLHNTMSRKKELFKPKVE-GKVGMYVCGVTAYDLSHIGHARVYVTFDVLYRYLRHLGY 133
           EL L+NT SR+KELF+P+V  G+VGMYVCGVT YD SHIGHAR YV FDVLYRYLR+L +
Sbjct: 71  ELHLYNTKSRRKELFQPRVPGGEVGMYVCGVTPYDDSHIGHARAYVAFDVLYRYLRYLDH 130

Query: 134 EVLYVRNFTDVDDKIIARANELGEDPLNLSKRYCEEFRQDMVYLHCLPPSVEPRVSDHMP 193
           +V YVRNFTD+DDKIIARAN+LGEDP +LSKRY ++F  DM  LHCLPPSVEPRVSDH+ 
Sbjct: 131 KVRYVRNFTDIDDKIIARANQLGEDPFSLSKRYSDDFLSDMANLHCLPPSVEPRVSDHID 190

Query: 194 QIVDMIKQILDNGYAYSVDGDVYFNVDKFPEYGLLSGRKLEDNRAGERVAVDSRKKNPAD 253
           QI++MIKQI+DN  AY++ GDVYF+V+ FPEYG LSGRKL+DNRAGERVAVD RKKNPAD
Sbjct: 191 QIINMIKQIIDNDCAYAIGGDVYFSVENFPEYGDLSGRKLDDNRAGERVAVDERKKNPAD 250

Query: 254 FALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHGGGMDLVFPHHENEIA 313
           FALWK+AK+GEP W+SPWGPGRPGWHIECSAMSA YLG+SFDIHGGG DL+FPHHENEIA
Sbjct: 251 FALWKAAKDGEPSWDSPWGPGRPGWHIECSAMSAHYLGHSFDIHGGGEDLIFPHHENEIA 310

Query: 314 QSCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYHPLALRLFLLGTHYRS 373
           QS AAC  S+I+YW+HNGFV ++S+KMSKSLGNF TIR+V +LYHPLALR+FLLGTHYRS
Sbjct: 311 QSRAACCDSSINYWIHNGFVNVNSQKMSKSLGNFVTIRKVTELYHPLALRMFLLGTHYRS 370

Query: 374 PINYSDFLLESASDRIYYIYQTLEDCRTVISQQDESSFKGSIAPSLAEEINKFYNVFLTS 433
           PINY+   L  ASDR+YY YQTL+DC     QQ +S     +  +    I K ++ F TS
Sbjct: 371 PINYTIEQLNVASDRLYYTYQTLQDCEE-SCQQHQSKAGDPLPVNTTNCIQKLHDEFETS 430

Query: 434 MSDDIHTPVVLAAISDPLKIINDLLHTRKGKKQESRMESLAALEKIIGSVLSILGLMPAS 493
           MSDD+HT V LAAIS+PLK++NDLLHTRKGKKQE R+ESL+A+E+ I  VLS+LGL+P+S
Sbjct: 431 MSDDLHTSVALAAISEPLKVMNDLLHTRKGKKQEKRLESLSAMEEKIRMVLSVLGLLPSS 490

Query: 494 YSEALQELKEKALTRAKMTNDQVVEKIEERNAARKNKEYEKSDAIRTDLAAVGITLMDGP 553
           Y EALQ+L+EKAL RA MT +QV++KIEER +ARK K+YEKSD IR +LAAVGI LMDGP
Sbjct: 491 YYEALQQLREKALRRASMTEEQVLQKIEERTSARKAKQYEKSDEIRKELAAVGIALMDGP 550

Query: 554 NGTTWRPAIPLALQ 567
           +GTTWRP++PL+ Q
Sbjct: 551 DGTTWRPSVPLSEQ 563

BLAST of Lag0037223 vs. ExPASy Swiss-Prot
Match: A0A1D6LAG9 (Cysteine--tRNA ligase CPS1, chloroplastic/mitochondrial OS=Zea mays OX=4577 GN=CPS1 PE=2 SV=1)

HSP 1 Score: 722.2 bits (1863), Expect = 4.5e-207
Identity = 352/501 (70.26%), Postives = 418/501 (83.43%), Query Frame = 0

Query: 74  ELWLHNTMSRKKELFKPKVEGK-VGMYVCGVTAYDLSHIGHARVYVTFDVLYRYLRHLGY 133
           EL L+NT SRKKE F+P++ G+ VGMYVCGVT YD SHIGHAR YV FDVLYRYLR+L Y
Sbjct: 65  ELRLYNTKSRKKEQFRPRIPGREVGMYVCGVTPYDDSHIGHARAYVAFDVLYRYLRYLDY 124

Query: 134 EVLYVRNFTDVDDKIIARANELGEDPLNLSKRYCEEFRQDMVYLHCLPPSVEPRVSDHMP 193
           EV YVRNFTD+DDKIIARAN+LGEDP +LSKR+ ++F  DM  L CLPPSVEPRVSDH+ 
Sbjct: 125 EVRYVRNFTDIDDKIIARANQLGEDPFSLSKRFSDDFLSDMANLQCLPPSVEPRVSDHVD 184

Query: 194 QIVDMIKQILDNGYAYSVDGDVYFNVDKFPEYGLLSGRKLEDNRAGERVAVDSRKKNPAD 253
           +I++MIKQILDN  AY V GDVYF+VD FPEYG LSGRKL+DNRAGERVAVD RK+NPAD
Sbjct: 185 EIINMIKQILDNRCAYVVGGDVYFSVDNFPEYGELSGRKLDDNRAGERVAVDERKRNPAD 244

Query: 254 FALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHGGGMDLVFPHHENEIA 313
           FALWK+AK+GEP+W+SPWGPGRPGWHIECSAMSA YLG+SFDIHGGG DL+FPHHENEIA
Sbjct: 245 FALWKAAKDGEPWWDSPWGPGRPGWHIECSAMSAHYLGHSFDIHGGGEDLIFPHHENEIA 304

Query: 314 QSCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYHPLALRLFLLGTHYRS 373
           QS AAC  S I+YW+HNGFV ++S+KMSKSLGNF TIR+VI++YHPLALR+FLLGTHYRS
Sbjct: 305 QSRAACCDSTINYWIHNGFVNVNSQKMSKSLGNFVTIRKVIEMYHPLALRMFLLGTHYRS 364

Query: 374 PINYSDFLLESASDRIYYIYQTLEDCRTVISQQDESSFKGSIAPSLAEEINKFYNVFLTS 433
           PINY+   L  ASDR+YY YQTL DC   I Q  +S+    +  +    I K ++ F TS
Sbjct: 365 PINYTIEQLNVASDRLYYTYQTLRDCEE-ICQHQQSNTGNPLPANTLNYIQKLHDEFETS 424

Query: 434 MSDDIHTPVVLAAISDPLKIINDLLHTRKGKKQESRMESLAALEKIIGSVLSILGLMPAS 493
           MSDD+HT V LAA+S+PLK++NDLLHTRKGKKQ+ R+ESL+ALE+ I  VLS+LGL+P+S
Sbjct: 425 MSDDLHTSVALAAMSEPLKVMNDLLHTRKGKKQDKRLESLSALEEKIRVVLSVLGLLPSS 484

Query: 494 YSEALQELKEKALTRAKMTNDQVVEKIEERNAARKNKEYEKSDAIRTDLAAVGITLMDGP 553
           Y EALQ+L++KAL RA +T + VV+KIEER AARK K+YEKSD IR +LAAVGI LMDGP
Sbjct: 485 YHEALQQLRDKALRRASITEELVVQKIEERTAARKAKQYEKSDEIRKELAAVGIALMDGP 544

Query: 554 NGTTWRPAIPLALQEQQAAST 574
           +GTTWRP++PL  +E   A T
Sbjct: 545 DGTTWRPSLPLPEEEAVLAKT 564

BLAST of Lag0037223 vs. ExPASy Swiss-Prot
Match: B3LFA4 (Cysteine--tRNA ligase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At5g38830 PE=2 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 1.5e-154
Identity = 282/503 (56.06%), Postives = 355/503 (70.58%), Query Frame = 0

Query: 69  ESTPSELWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVYVTFDVLYRYLR 128
           E+   EL L+NTM+++KE+  P   GK+G+YVCG+TAYD SHIGHAR  V+FDVLYRYL+
Sbjct: 2   EAEKMELKLYNTMTQQKEVLIPITPGKIGLYVCGITAYDFSHIGHARAAVSFDVLYRYLK 61

Query: 129 HLGYEVLYVRNFTDVDDKIIARANELGEDPLNLSKRYCEEFRQDMVYLHCLPPSVEPRVS 188
           HL Y+V +VRNFTDVDDKII RAN+ GEDPL+LS R+C+E+  DM  L CLPP+ +PRVS
Sbjct: 62  HLDYDVTFVRNFTDVDDKIIDRANKNGEDPLDLSNRFCDEYLVDMGALQCLPPTHQPRVS 121

Query: 189 DHMPQIVDMIKQILDNGYAYSVDGDVYFNVDKFPEYGLLSGRKLEDNRAGERVAVDSRKK 248
           +HM  I+ MI++I++    Y V+GDV+F+VDK P YG LSG+ LE  RAGERVAVDSRK+
Sbjct: 122 EHMDNIIKMIEKIIEKDCGYVVEGDVFFSVDKSPNYGKLSGQLLEHTRAGERVAVDSRKR 181

Query: 249 NPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHGGGMDLVFPHHE 308
           NPADFALWK+AK  EP WESPWGPGRPGWHIECSAMS  YL   FDIHGGG DL FPHHE
Sbjct: 182 NPADFALWKAAKPDEPSWESPWGPGRPGWHIECSAMSVHYLSPKFDIHGGGADLKFPHHE 241

Query: 309 NEIAQSCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYHPLALRLFLLGT 368
           NEIAQ+CAAC  S ++YW+HNG VTI++EKM+KS  NF TIR++   YHPLALR FL+  
Sbjct: 242 NEIAQTCAACEDSGVNYWLHNGHVTINNEKMAKSKHNFKTIREITASYHPLALRHFLMSA 301

Query: 369 HYRSPINYSDFLLESASDRIYYIYQTLEDCRTVIS-QQDESSFKGSIAPSLAEE---INK 428
            YRSP++++   LES+S+ +YY+YQTL+D    +S  QD  S  G  +   AE    I K
Sbjct: 302 QYRSPLSFTASQLESSSEALYYVYQTLQDLDEGLSPYQDALSEDGGKSEQTAEGKDIIKK 361

Query: 429 FYNVFLTSMSDDIHTPVVL-AAISDPLKIINDLLHTRKGKKQESRME---SLAALEKIIG 488
               F + M DD++T  +L  A  D LK IN  L   K  +++ RM    SL  +EK   
Sbjct: 362 LKTEFESKMLDDLNTAHILTGAYQDALKFINASLSKLKKMQKKQRMSMLVSLVEIEKAAR 421

Query: 489 SVLSILGLMPA-SYSEALQELKEKALTRAKMTNDQVVEKIEERNAARKNKEYEKSDAIRT 548
            VL +LGL+   SY+E L+E+K K L RA++  + + + IEER  ARKNK++ KSD IR 
Sbjct: 422 EVLDVLGLLTTLSYAEILKEMKLKTLIRAEIGEEGISQLIEERITARKNKDFAKSDEIRE 481

Query: 549 DLAAVGITLMDGPNGTTWRPAIP 563
            L   GI LMD    T WRP  P
Sbjct: 482 KLTRKGIALMDIGKETVWRPCFP 504

BLAST of Lag0037223 vs. ExPASy Swiss-Prot
Match: Q9LYL3 (Cysteine--tRNA ligase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g56300 PE=3 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 3.1e-139
Identity = 261/494 (52.83%), Postives = 334/494 (67.61%), Query Frame = 0

Query: 74  ELWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVYVTFDVLYRYLRHLGYE 133
           +L L+NTM++ KE++KP   GK+G+YVCG+TAYD SHIGHAR  V+FD+LYRYLRHLGY+
Sbjct: 10  DLTLYNTMTQLKEVYKPMNPGKIGIYVCGITAYDYSHIGHARAAVSFDLLYRYLRHLGYQ 69

Query: 134 VLYVRNFTDVDDKIIARANELGEDPLNLSKRYCEEFRQDMVYLHCLPPSVEPRVSDHMPQ 193
           V YVRNFTDVDDK    A   GE PL+LS R+CEE+  DM  L CL P+ +PRVSDHM Q
Sbjct: 70  VTYVRNFTDVDDK----AKNCGEKPLDLSNRFCEEYLLDMAALQCLLPTHQPRVSDHMEQ 129

Query: 194 IVDMIKQILDNGYAYSVDGDVYFNVDKFPEYGLLSGRKLEDNRAGERVAVDSRKKNPADF 253
           I+ MI++I++NG  Y+V GDV+F+VDK P YG LSG++L+  +AG+RVAVDSRK+NPADF
Sbjct: 130 IIKMIEKIIENGCGYAVGGDVFFSVDKSPSYGQLSGQRLDHTQAGKRVAVDSRKRNPADF 189

Query: 254 ALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHGGGMDLVFPHHENEIAQ 313
           AL K+AK GEP WESPWG GRPGWHIE            FDIHGGG DL FPHHENEIAQ
Sbjct: 190 ALRKAAKSGEPSWESPWGHGRPGWHIE------------FDIHGGGADLKFPHHENEIAQ 249

Query: 314 SCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYHPLALRLFLLGTHYRSP 373
           +CAAC  S ++YW+HNG VT ++ KM KSL NFFTIRQ+   YHPLALR FL+   YRSP
Sbjct: 250 TCAACEDSGVNYWLHNGHVTNNNVKMGKSLNNFFTIRQIAANYHPLALRHFLMSAQYRSP 309

Query: 374 INYSDFLLESASDRIYYIYQTLEDCRTVISQQDESSFKGSIAPSLAEEINKFYNVFLTSM 433
           +NYS   LES+SD +Y +    E+    + +  +S+          E I K         
Sbjct: 310 LNYSVSQLESSSDALYSLSPYREEMSGDVGKTQQSA-------EAKEMIKK--------- 369

Query: 434 SDDIHTPVVLAAISDPLKIINDLLHTRKGKKQESRME---SLAALEKIIGSVLSILGLMP 493
                       + + LK IN  +   K  +++ RM    SL  +EK +  VL +LGL+ 
Sbjct: 370 ------------VKNALKFINVSISKLKKMQKKQRMSLVVSLVEVEKAVREVLDVLGLLT 429

Query: 494 A-SYSEALQELKEKALTRAKMTNDQVVEKIEERNAARKNKEYEKSDAIRTDLAAVGITLM 553
             SY E L+++K+KALTRA M  ++V+++IEERN ARK+K++ +SD IR  LA  GI L 
Sbjct: 430 TLSYGELLKDMKQKALTRAGMGEEEVLQRIEERNMARKSKDFRRSDRIRELLAFKGIFLE 459

Query: 554 DGPNGTTWRPAIPL 564
           D P  T WRP+ PL
Sbjct: 490 DVPGDTVWRPSTPL 459

BLAST of Lag0037223 vs. ExPASy TrEMBL
Match: A0A5D3E6A8 (Cysteinyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G007660 PE=3 SV=1)

HSP 1 Score: 1052.4 bits (2720), Expect = 7.0e-304
Identity = 520/576 (90.28%), Postives = 545/576 (94.62%), Query Frame = 0

Query: 1   MASLLKFYNPSTFTRFSPISVSLAILRRTHRHSRFPFFKSA---RCFATSLSSPQHPVCE 60
           MASLLKFYNP TFTRF+PISVS  ILRRT R+S F FF SA    CF +S +S Q PV  
Sbjct: 1   MASLLKFYNPLTFTRFTPISVSQVILRRTLRNSHFSFFNSATTFACFTSSPTSSQLPVSA 60

Query: 61  ENHKEIGGVRRESTPSELWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVY 120
            N K+I  VR EST SELWLHNTMSRKKE+FKPKVEGKVGMYVCGVTAYDLSHIGHARVY
Sbjct: 61  PNLKKINAVRHESTSSELWLHNTMSRKKEVFKPKVEGKVGMYVCGVTAYDLSHIGHARVY 120

Query: 121 VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLNLSKRYCEEFRQDMVYLH 180
           VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLNLS+RYCEEFR+DMVYLH
Sbjct: 121 VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLNLSRRYCEEFRRDMVYLH 180

Query: 181 CLPPSVEPRVSDHMPQIVDMIKQILDNGYAYSVDGDVYFNVDKFPEYGLLSGRKLEDNRA 240
           CLPPSVEP+VSDHMPQI+DMIKQILDNGYAY VDGDVYFNVDKFP+YG LSGRKLEDNRA
Sbjct: 181 CLPPSVEPQVSDHMPQIIDMIKQILDNGYAYCVDGDVYFNVDKFPDYGQLSGRKLEDNRA 240

Query: 241 GERVAVDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHG 300
           GERV+VDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSA+YLGYSFDIHG
Sbjct: 241 GERVSVDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSASYLGYSFDIHG 300

Query: 301 GGMDLVFPHHENEIAQSCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH 360
           GGMDLVFPHHENEIAQSCAACRTSN+SYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH
Sbjct: 301 GGMDLVFPHHENEIAQSCAACRTSNVSYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH 360

Query: 361 PLALRLFLLGTHYRSPINYSDFLLESASDRIYYIYQTLEDCRTVISQQDESSFKGSIAPS 420
           PLALRLFLLGTHYRSPINYSD LLESASDRI+YIYQTL+DCRTVISQQDESSFKGSIAPS
Sbjct: 361 PLALRLFLLGTHYRSPINYSDLLLESASDRIFYIYQTLDDCRTVISQQDESSFKGSIAPS 420

Query: 421 LAEEINKFYNVFLTSMSDDIHTPVVLAAISDPLKIINDLLHTRKGKKQESRMESLAALEK 480
           L EEINKFYNVFLTSMSDDIHTPVVLAA+SDPLKIINDLLHTRKGKKQE RMESLAALEK
Sbjct: 421 LMEEINKFYNVFLTSMSDDIHTPVVLAALSDPLKIINDLLHTRKGKKQEFRMESLAALEK 480

Query: 481 IIGSVLSILGLMPASYSEALQELKEKALTRAKMTNDQVVEKIEERNAARKNKEYEKSDAI 540
           IIG+VLSILGLMPASYSEALQ+LKEKAL RAKMTNDQV++KIEERNAARKNKEYEKSD+I
Sbjct: 481 IIGNVLSILGLMPASYSEALQQLKEKALKRAKMTNDQVLQKIEERNAARKNKEYEKSDSI 540

Query: 541 RTDLAAVGITLMDGPNGTTWRPAIPLALQEQQAAST 574
           RTDLAAVGI LMDGPNGTTWRP++PLALQE QA+ST
Sbjct: 541 RTDLAAVGIALMDGPNGTTWRPSVPLALQEHQASST 576

BLAST of Lag0037223 vs. ExPASy TrEMBL
Match: A0A1S3CSJ7 (Cysteinyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503847 PE=3 SV=1)

HSP 1 Score: 1052.4 bits (2720), Expect = 7.0e-304
Identity = 520/576 (90.28%), Postives = 545/576 (94.62%), Query Frame = 0

Query: 1   MASLLKFYNPSTFTRFSPISVSLAILRRTHRHSRFPFFKSA---RCFATSLSSPQHPVCE 60
           MASLLKFYNP TFTRF+PISVS  ILRRT R+S F FF SA    CF +S +S Q PV  
Sbjct: 1   MASLLKFYNPLTFTRFTPISVSQVILRRTLRNSHFSFFNSATTFACFTSSPTSSQLPVSA 60

Query: 61  ENHKEIGGVRRESTPSELWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVY 120
            N K+I  VR EST SELWLHNTMSRKKE+FKPKVEGKVGMYVCGVTAYDLSHIGHARVY
Sbjct: 61  PNLKKINAVRHESTSSELWLHNTMSRKKEVFKPKVEGKVGMYVCGVTAYDLSHIGHARVY 120

Query: 121 VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLNLSKRYCEEFRQDMVYLH 180
           VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLNLS+RYCEEFR+DMVYLH
Sbjct: 121 VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLNLSRRYCEEFRRDMVYLH 180

Query: 181 CLPPSVEPRVSDHMPQIVDMIKQILDNGYAYSVDGDVYFNVDKFPEYGLLSGRKLEDNRA 240
           CLPPSVEP+VSDHMPQI+DMIKQILDNGYAY VDGDVYFNVDKFP+YG LSGRKLEDNRA
Sbjct: 181 CLPPSVEPQVSDHMPQIIDMIKQILDNGYAYCVDGDVYFNVDKFPDYGQLSGRKLEDNRA 240

Query: 241 GERVAVDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHG 300
           GERV+VDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSA+YLGYSFDIHG
Sbjct: 241 GERVSVDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSASYLGYSFDIHG 300

Query: 301 GGMDLVFPHHENEIAQSCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH 360
           GGMDLVFPHHENEIAQSCAACRTSN+SYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH
Sbjct: 301 GGMDLVFPHHENEIAQSCAACRTSNVSYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH 360

Query: 361 PLALRLFLLGTHYRSPINYSDFLLESASDRIYYIYQTLEDCRTVISQQDESSFKGSIAPS 420
           PLALRLFLLGTHYRSPINYSD LLESASDRI+YIYQTL+DCRTVISQQDESSFKGSIAPS
Sbjct: 361 PLALRLFLLGTHYRSPINYSDLLLESASDRIFYIYQTLDDCRTVISQQDESSFKGSIAPS 420

Query: 421 LAEEINKFYNVFLTSMSDDIHTPVVLAAISDPLKIINDLLHTRKGKKQESRMESLAALEK 480
           L EEINKFYNVFLTSMSDDIHTPVVLAA+SDPLKIINDLLHTRKGKKQE RMESLAALEK
Sbjct: 421 LMEEINKFYNVFLTSMSDDIHTPVVLAALSDPLKIINDLLHTRKGKKQEFRMESLAALEK 480

Query: 481 IIGSVLSILGLMPASYSEALQELKEKALTRAKMTNDQVVEKIEERNAARKNKEYEKSDAI 540
           IIG+VLSILGLMPASYSEALQ+LKEKAL RAKMTNDQV++KIEERNAARKNKEYEKSD+I
Sbjct: 481 IIGNVLSILGLMPASYSEALQQLKEKALKRAKMTNDQVLQKIEERNAARKNKEYEKSDSI 540

Query: 541 RTDLAAVGITLMDGPNGTTWRPAIPLALQEQQAAST 574
           RTDLAAVGI LMDGPNGTTWRP++PLALQE QA+ST
Sbjct: 541 RTDLAAVGIALMDGPNGTTWRPSVPLALQEHQASST 576

BLAST of Lag0037223 vs. ExPASy TrEMBL
Match: A0A5A7VBP9 (Cysteinyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold205G001510 PE=3 SV=1)

HSP 1 Score: 1052.0 bits (2719), Expect = 9.2e-304
Identity = 520/576 (90.28%), Postives = 545/576 (94.62%), Query Frame = 0

Query: 1   MASLLKFYNPSTFTRFSPISVSLAILRRTHRHSRFPFFKSA---RCFATSLSSPQHPVCE 60
           MASLLKFYNP TFTRF+PISVS  ILRRT R+S F FF SA    CF +S +S Q PV  
Sbjct: 1   MASLLKFYNPLTFTRFTPISVSQVILRRTLRNSHFSFFNSATTFACFTSSPTSSQLPVSA 60

Query: 61  ENHKEIGGVRRESTPSELWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVY 120
            N K+I  VR EST SELWLHNTMSRKKE+FKPKVEGKVGMYVCGVTAYDLSHIGHARVY
Sbjct: 61  PNLKKINAVRHESTSSELWLHNTMSRKKEVFKPKVEGKVGMYVCGVTAYDLSHIGHARVY 120

Query: 121 VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLNLSKRYCEEFRQDMVYLH 180
           VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLNLS+RYCEEFR+DMVYLH
Sbjct: 121 VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLNLSRRYCEEFRRDMVYLH 180

Query: 181 CLPPSVEPRVSDHMPQIVDMIKQILDNGYAYSVDGDVYFNVDKFPEYGLLSGRKLEDNRA 240
           CLPPSVEP+VSDHMPQI+DMIKQILDNGYAY VDGDVYFNVDKFP+YG LSGRKLEDNRA
Sbjct: 181 CLPPSVEPQVSDHMPQIIDMIKQILDNGYAYCVDGDVYFNVDKFPDYGQLSGRKLEDNRA 240

Query: 241 GERVAVDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHG 300
           GERV+VDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSA+YLGYSFDIHG
Sbjct: 241 GERVSVDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSASYLGYSFDIHG 300

Query: 301 GGMDLVFPHHENEIAQSCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH 360
           GGMDLVFPHHENEIAQSCAACRTSN+SYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH
Sbjct: 301 GGMDLVFPHHENEIAQSCAACRTSNVSYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH 360

Query: 361 PLALRLFLLGTHYRSPINYSDFLLESASDRIYYIYQTLEDCRTVISQQDESSFKGSIAPS 420
           PLALRLFLLGTHYRSPINYSD LLESASDRI+YIYQTL+DCRTVISQQDESSFKGSIAPS
Sbjct: 361 PLALRLFLLGTHYRSPINYSDLLLESASDRIFYIYQTLDDCRTVISQQDESSFKGSIAPS 420

Query: 421 LAEEINKFYNVFLTSMSDDIHTPVVLAAISDPLKIINDLLHTRKGKKQESRMESLAALEK 480
           L EEINKFYNVFLTSMSDDIHTPVVLAA+SDPLKIINDLLHTRKGKKQE RMESLAALEK
Sbjct: 421 LMEEINKFYNVFLTSMSDDIHTPVVLAALSDPLKIINDLLHTRKGKKQEFRMESLAALEK 480

Query: 481 IIGSVLSILGLMPASYSEALQELKEKALTRAKMTNDQVVEKIEERNAARKNKEYEKSDAI 540
           IIG+VLSILGLMPASYSEALQ+LKEKAL RAKMTNDQV++KIEERNAARKNKEYEKSD+I
Sbjct: 481 IIGNVLSILGLMPASYSEALQQLKEKALKRAKMTNDQVLQKIEERNAARKNKEYEKSDSI 540

Query: 541 RTDLAAVGITLMDGPNGTTWRPAIPLALQEQQAAST 574
           RTDLAAVGI LMDGPNGTTWRP++PLALQE QA+ST
Sbjct: 541 RTDLAAVGIALMDGPNGTTWRPSLPLALQEHQASST 576

BLAST of Lag0037223 vs. ExPASy TrEMBL
Match: A0A6J1IMQ6 (Cysteinyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111476622 PE=3 SV=1)

HSP 1 Score: 1051.6 bits (2718), Expect = 1.2e-303
Identity = 515/570 (90.35%), Postives = 537/570 (94.21%), Query Frame = 0

Query: 1   MASLLKFYNPSTFTRFSPISVSLAILRRTHRHSRFPFFKSARCFATSLSSP---QHPVCE 60
           MASLLKFYNP +FTR SP+S+S AI RR HRHS FPFFK ARCFA   SSP   QHP+  
Sbjct: 1   MASLLKFYNPLSFTRCSPVSISQAIFRRAHRHSHFPFFKPARCFACFTSSPSSSQHPLSA 60

Query: 61  ENHKEIGGVRRESTPSELWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVY 120
           +NHKEIGGVR ES  SELWLHNTMSRKKE+FKPKVEGKVGMYVCGVTAYDLSHIGHARVY
Sbjct: 61  QNHKEIGGVRHESPSSELWLHNTMSRKKEVFKPKVEGKVGMYVCGVTAYDLSHIGHARVY 120

Query: 121 VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLNLSKRYCEEFRQDMVYLH 180
           VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPL+LS+RYCEEF QDM YLH
Sbjct: 121 VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLDLSRRYCEEFGQDMRYLH 180

Query: 181 CLPPSVEPRVSDHMPQIVDMIKQILDNGYAYSVDGDVYFNVDKFPEYGLLSGRKLEDNRA 240
           CL PSVEPRVSDHMPQI+DMIKQILDNGYAY VDGDVYFNVDKFPEYGLLSGRKLEDNRA
Sbjct: 181 CLSPSVEPRVSDHMPQIIDMIKQILDNGYAYRVDGDVYFNVDKFPEYGLLSGRKLEDNRA 240

Query: 241 GERVAVDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHG 300
           GERVAVDSRKKNPADFALWKSAKEGEPFW SPWGPGRPGWHIECSAMSA YLGYSFDIHG
Sbjct: 241 GERVAVDSRKKNPADFALWKSAKEGEPFWASPWGPGRPGWHIECSAMSAAYLGYSFDIHG 300

Query: 301 GGMDLVFPHHENEIAQSCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH 360
           GGMDLVFPHHENEIAQSCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH
Sbjct: 301 GGMDLVFPHHENEIAQSCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH 360

Query: 361 PLALRLFLLGTHYRSPINYSDFLLESASDRIYYIYQTLEDCRTVISQQDESSFKGSIAPS 420
           PLALRLFLLGTHYRSPINYSD LLESASDRI+YIYQTL+DCRTVISQQDESS K SI PS
Sbjct: 361 PLALRLFLLGTHYRSPINYSDLLLESASDRIFYIYQTLDDCRTVISQQDESSLKSSIPPS 420

Query: 421 LAEEINKFYNVFLTSMSDDIHTPVVLAAISDPLKIINDLLHTRKGKKQESRMESLAALEK 480
           L EEINKFYNVFLTSMSDDIHTPVVLAA+SDPLKIINDLLHTRKGKKQESRMESLAALEK
Sbjct: 421 LVEEINKFYNVFLTSMSDDIHTPVVLAALSDPLKIINDLLHTRKGKKQESRMESLAALEK 480

Query: 481 IIGSVLSILGLMPASYSEALQELKEKALTRAKMTNDQVVEKIEERNAARKNKEYEKSDAI 540
           +IG+VLSILGLMPASYSEALQ+LKE ALTRAKMTN+QV++KIEERNAAR+NKEYEKSD I
Sbjct: 481 MIGNVLSILGLMPASYSEALQQLKEMALTRAKMTNEQVLQKIEERNAARQNKEYEKSDTI 540

Query: 541 RTDLAAVGITLMDGPNGTTWRPAIPLALQE 568
           RTDLAAVGI LMDGPNGTTWRP +P+ALQ+
Sbjct: 541 RTDLAAVGIALMDGPNGTTWRPTVPIALQD 570

BLAST of Lag0037223 vs. ExPASy TrEMBL
Match: A0A6J1C872 (Cysteinyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111008869 PE=3 SV=1)

HSP 1 Score: 1051.6 bits (2718), Expect = 1.2e-303
Identity = 521/576 (90.45%), Postives = 542/576 (94.10%), Query Frame = 0

Query: 1   MASLLKFYNPSTFTRFSPISVSLAILRRTHRHSRFPFFKSAR---CFATSLSSPQHPVCE 60
           MASLLKFY P TFT+FSPI  S  ILRRT R+S F FF S R   CF TSLSS Q PV E
Sbjct: 1   MASLLKFYKPLTFTQFSPIRFSQTILRRTRRNSHFSFFNSGRTFACFTTSLSSSQQPVRE 60

Query: 61  ENHKEIGGVRRESTPSELWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVY 120
           +N KEIG V RES  SELWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVY
Sbjct: 61  KNCKEIGVVGRESMWSELWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVY 120

Query: 121 VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLNLSKRYCEEFRQDMVYLH 180
           VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLNLSKRYCEEF QDMVYLH
Sbjct: 121 VTFDVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLNLSKRYCEEFCQDMVYLH 180

Query: 181 CLPPSVEPRVSDHMPQIVDMIKQILDNGYAYSVDGDVYFNVDKFPEYGLLSGRKLEDNRA 240
           CLPPSVEPRVSDHMPQI+DMIKQILDNGYAY V+GDVYFNVDKFP+YGLLSGRKLEDNRA
Sbjct: 181 CLPPSVEPRVSDHMPQIIDMIKQILDNGYAYCVEGDVYFNVDKFPKYGLLSGRKLEDNRA 240

Query: 241 GERVAVDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHG 300
           GERVAVDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHG
Sbjct: 241 GERVAVDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHG 300

Query: 301 GGMDLVFPHHENEIAQSCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH 360
           GGMDLVFPHHENEIAQSCAACRTSNISYW+HNGFVTIDSEKMSKSLGNFFTIRQVIDLYH
Sbjct: 301 GGMDLVFPHHENEIAQSCAACRTSNISYWIHNGFVTIDSEKMSKSLGNFFTIRQVIDLYH 360

Query: 361 PLALRLFLLGTHYRSPINYSDFLLESASDRIYYIYQTLEDCRTVISQQDESSFKGSIAPS 420
           PLALRLFLLGTHYRSPINYSD LLESASDRI+YIYQTL+DCR V+SQQDESSFKGSIAPS
Sbjct: 361 PLALRLFLLGTHYRSPINYSDLLLESASDRIFYIYQTLDDCRAVVSQQDESSFKGSIAPS 420

Query: 421 LAEEINKFYNVFLTSMSDDIHTPVVLAAISDPLKIINDLLHTRKGKKQESRMESLAALEK 480
           + EEIN+FYNV LTSMSDDIHTPVVLAA+SDPLKIINDLLHTRKGKKQE R+ESLAALEK
Sbjct: 421 VVEEINRFYNVVLTSMSDDIHTPVVLAALSDPLKIINDLLHTRKGKKQEFRIESLAALEK 480

Query: 481 IIGSVLSILGLMPASYSEALQELKEKALTRAKMTNDQVVEKIEERNAARKNKEYEKSDAI 540
           IIGSVLSILGLMPASYSEALQ+LKEKAL RAK+TNDQV++KIEERNAARKNKEYEKSDAI
Sbjct: 481 IIGSVLSILGLMPASYSEALQQLKEKALIRAKLTNDQVLQKIEERNAARKNKEYEKSDAI 540

Query: 541 RTDLAAVGITLMDGPNGTTWRPAIPLALQEQQAAST 574
           RTDLA VGI+LMDGPNGTTWRP +PL+LQE QAAST
Sbjct: 541 RTDLATVGISLMDGPNGTTWRPTVPLSLQEHQAAST 576

BLAST of Lag0037223 vs. TAIR 10
Match: AT2G31170.1 (Cysteinyl-tRNA synthetase, class Ia family protein )

HSP 1 Score: 815.5 bits (2105), Expect = 2.8e-236
Identity = 408/567 (71.96%), Postives = 459/567 (80.95%), Query Frame = 0

Query: 3   SLLKFYNPSTFTRFSPISVSLAILRRTHRHSRFPFFKSARCFAT--SLSSPQHPVCEENH 62
           +L K   P T  RFS +  S   ++   R  +    +  RCF T  SL+    P+     
Sbjct: 7   NLFKSCRPFTPIRFSSLPKSQFRIQFPLRPGKETQLR--RCFTTLSSLTDGGAPI----- 66

Query: 63  KEIGGVRRESTPSELWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVYVTF 122
                    S   ELWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVYVTF
Sbjct: 67  ---------SGGKELWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVYVTF 126

Query: 123 DVLYRYLRHLGYEVLYVRNFTDVDDKIIARANELGEDPLNLSKRYCEEFRQDMVYLHCLP 182
           DVL RYL+HLGYEV YVRNFTDVDDKIIARA EL EDP++LS+R+CEEF +DM  L CL 
Sbjct: 127 DVLLRYLKHLGYEVSYVRNFTDVDDKIIARAKELEEDPISLSRRFCEEFNRDMEQLQCLD 186

Query: 183 PSVEPRVSDHMPQIVDMIKQILDNGYAYSVDGDVYFNVDKFPEYGLLSGRKLEDNRAGER 242
           PSV+PRVSDH+PQI+D+IKQILDNGYAY VDGD+YF+VDKFP YG LSGRKLEDNRAGER
Sbjct: 187 PSVQPRVSDHIPQIIDLIKQILDNGYAYKVDGDIYFSVDKFPTYGKLSGRKLEDNRAGER 246

Query: 243 VAVDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHGGGM 302
           VAVD+RKK+PADFALWK+AKEGEPFWESPWG GRPGWHIECSAMSA YLGYSFDIHGGGM
Sbjct: 247 VAVDTRKKHPADFALWKAAKEGEPFWESPWGRGRPGWHIECSAMSAAYLGYSFDIHGGGM 306

Query: 303 DLVFPHHENEIAQSCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYHPLA 362
           DLVFPHHENEIAQSCAAC +SNISYW+HNGFVT+DSEKMSKSLGNFFTIRQVIDLYHPLA
Sbjct: 307 DLVFPHHENEIAQSCAACDSSNISYWIHNGFVTVDSEKMSKSLGNFFTIRQVIDLYHPLA 366

Query: 363 LRLFLLGTHYRSPINYSDFLLESASDRIYYIYQTLEDCRTVISQQDESSFKGSIAPSLAE 422
           LRLFL+GTHYRSPINYSDFLLESAS+RI+YIYQTL DC + + ++D +   GS+      
Sbjct: 367 LRLFLMGTHYRSPINYSDFLLESASERIFYIYQTLHDCESALGEKDSTFENGSVPSDTLT 426

Query: 423 EINKFYNVFLTSMSDDIHTPVVLAAISDPLKIINDLLHTRKGKKQESRMESLAALEKIIG 482
            IN F   F+ SMSDD+ TPV LAA+S+PLK INDL+HTRKGKKQ  R ESL ALE  I 
Sbjct: 427 SINTFRTEFVASMSDDLLTPVTLAAMSEPLKTINDLIHTRKGKKQARREESLKALETTIR 486

Query: 483 SVLSILGLMPASYSEALQELKEKALTRAKMTNDQVVEKIEERNAARKNKEYEKSDAIRTD 542
            VL+ILGLMP SYSE L++LKEKAL RA +  + V+++++ER  ARKNKEYE+SDAIR D
Sbjct: 487 DVLTILGLMPTSYSEVLEQLKEKALKRAGLKEEDVLQRVQERTDARKNKEYERSDAIRKD 546

Query: 543 LAAVGITLMDGPNGTTWRPAIPLALQE 568
           LA VGI LMD P GTTWRPAIPLALQE
Sbjct: 547 LAKVGIALMDSPEGTTWRPAIPLALQE 557

BLAST of Lag0037223 vs. TAIR 10
Match: AT2G31170.2 (Cysteinyl-tRNA synthetase, class Ia family protein )

HSP 1 Score: 630.6 bits (1625), Expect = 1.3e-180
Identity = 303/395 (76.71%), Postives = 340/395 (86.08%), Query Frame = 0

Query: 173 MVYLHCLPPSVEPRVSDHMPQIVDMIKQILDNGYAYSVDGDVYFNVDKFPEYGLLSGRKL 232
           M  L CL PSV+PRVSDH+PQI+D+IKQILDNGYAY VDGD+YF+VDKFP YG LSGRKL
Sbjct: 1   MEQLQCLDPSVQPRVSDHIPQIIDLIKQILDNGYAYKVDGDIYFSVDKFPTYGKLSGRKL 60

Query: 233 EDNRAGERVAVDSRKKNPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYS 292
           EDNRAGERVAVD+RKK+PADFALWK+AKEGEPFWESPWG GRPGWHIECSAMSA YLGYS
Sbjct: 61  EDNRAGERVAVDTRKKHPADFALWKAAKEGEPFWESPWGRGRPGWHIECSAMSAAYLGYS 120

Query: 293 FDIHGGGMDLVFPHHENEIAQSCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQV 352
           FDIHGGGMDLVFPHHENEIAQSCAAC +SNISYW+HNGFVT+DSEKMSKSLGNFFTIRQV
Sbjct: 121 FDIHGGGMDLVFPHHENEIAQSCAACDSSNISYWIHNGFVTVDSEKMSKSLGNFFTIRQV 180

Query: 353 IDLYHPLALRLFLLGTHYRSPINYSDFLLESASDRIYYIYQTLEDCRTVISQQDESSFKG 412
           IDLYHPLALRLFL+GTHYRSPINYSDFLLESAS+RI+YIYQTL DC + + ++D +   G
Sbjct: 181 IDLYHPLALRLFLMGTHYRSPINYSDFLLESASERIFYIYQTLHDCESALGEKDSTFENG 240

Query: 413 SIAPSLAEEINKFYNVFLTSMSDDIHTPVVLAAISDPLKIINDLLHTRKGKKQESRMESL 472
           S+       IN F   F+ SMSDD+ TPV LAA+S+PLK INDL+HTRKGKKQ  R ESL
Sbjct: 241 SVPSDTLTSINTFRTEFVASMSDDLLTPVTLAAMSEPLKTINDLIHTRKGKKQARREESL 300

Query: 473 AALEKIIGSVLSILGLMPASYSEALQELKEKALTRAKMTNDQVVEKIEERNAARKNKEYE 532
            ALE  I  VL+ILGLMP SYSE L++LKEKAL RA +  + V+++++ER  ARKNKEYE
Sbjct: 301 KALETTIRDVLTILGLMPTSYSEVLEQLKEKALKRAGLKEEDVLQRVQERTDARKNKEYE 360

Query: 533 KSDAIRTDLAAVGITLMDGPNGTTWRPAIPLALQE 568
           +SDAIR DLA VGI LMD P GTTWRPAIPLALQE
Sbjct: 361 RSDAIRKDLAKVGIALMDSPEGTTWRPAIPLALQE 395

BLAST of Lag0037223 vs. TAIR 10
Match: AT5G38830.1 (Cysteinyl-tRNA synthetase, class Ia family protein )

HSP 1 Score: 547.7 bits (1410), Expect = 1.1e-155
Identity = 282/503 (56.06%), Postives = 355/503 (70.58%), Query Frame = 0

Query: 69  ESTPSELWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVYVTFDVLYRYLR 128
           E+   EL L+NTM+++KE+  P   GK+G+YVCG+TAYD SHIGHAR  V+FDVLYRYL+
Sbjct: 2   EAEKMELKLYNTMTQQKEVLIPITPGKIGLYVCGITAYDFSHIGHARAAVSFDVLYRYLK 61

Query: 129 HLGYEVLYVRNFTDVDDKIIARANELGEDPLNLSKRYCEEFRQDMVYLHCLPPSVEPRVS 188
           HL Y+V +VRNFTDVDDKII RAN+ GEDPL+LS R+C+E+  DM  L CLPP+ +PRVS
Sbjct: 62  HLDYDVTFVRNFTDVDDKIIDRANKNGEDPLDLSNRFCDEYLVDMGALQCLPPTHQPRVS 121

Query: 189 DHMPQIVDMIKQILDNGYAYSVDGDVYFNVDKFPEYGLLSGRKLEDNRAGERVAVDSRKK 248
           +HM  I+ MI++I++    Y V+GDV+F+VDK P YG LSG+ LE  RAGERVAVDSRK+
Sbjct: 122 EHMDNIIKMIEKIIEKDCGYVVEGDVFFSVDKSPNYGKLSGQLLEHTRAGERVAVDSRKR 181

Query: 249 NPADFALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHGGGMDLVFPHHE 308
           NPADFALWK+AK  EP WESPWGPGRPGWHIECSAMS  YL   FDIHGGG DL FPHHE
Sbjct: 182 NPADFALWKAAKPDEPSWESPWGPGRPGWHIECSAMSVHYLSPKFDIHGGGADLKFPHHE 241

Query: 309 NEIAQSCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYHPLALRLFLLGT 368
           NEIAQ+CAAC  S ++YW+HNG VTI++EKM+KS  NF TIR++   YHPLALR FL+  
Sbjct: 242 NEIAQTCAACEDSGVNYWLHNGHVTINNEKMAKSKHNFKTIREITASYHPLALRHFLMSA 301

Query: 369 HYRSPINYSDFLLESASDRIYYIYQTLEDCRTVIS-QQDESSFKGSIAPSLAEE---INK 428
            YRSP++++   LES+S+ +YY+YQTL+D    +S  QD  S  G  +   AE    I K
Sbjct: 302 QYRSPLSFTASQLESSSEALYYVYQTLQDLDEGLSPYQDALSEDGGKSEQTAEGKDIIKK 361

Query: 429 FYNVFLTSMSDDIHTPVVL-AAISDPLKIINDLLHTRKGKKQESRME---SLAALEKIIG 488
               F + M DD++T  +L  A  D LK IN  L   K  +++ RM    SL  +EK   
Sbjct: 362 LKTEFESKMLDDLNTAHILTGAYQDALKFINASLSKLKKMQKKQRMSMLVSLVEIEKAAR 421

Query: 489 SVLSILGLMPA-SYSEALQELKEKALTRAKMTNDQVVEKIEERNAARKNKEYEKSDAIRT 548
            VL +LGL+   SY+E L+E+K K L RA++  + + + IEER  ARKNK++ KSD IR 
Sbjct: 422 EVLDVLGLLTTLSYAEILKEMKLKTLIRAEIGEEGISQLIEERITARKNKDFAKSDEIRE 481

Query: 549 DLAAVGITLMDGPNGTTWRPAIP 563
            L   GI LMD    T WRP  P
Sbjct: 482 KLTRKGIALMDIGKETVWRPCFP 504

BLAST of Lag0037223 vs. TAIR 10
Match: AT3G56300.1 (Cysteinyl-tRNA synthetase, class Ia family protein )

HSP 1 Score: 496.9 bits (1278), Expect = 2.2e-140
Identity = 261/494 (52.83%), Postives = 334/494 (67.61%), Query Frame = 0

Query: 74  ELWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVYVTFDVLYRYLRHLGYE 133
           +L L+NTM++ KE++KP   GK+G+YVCG+TAYD SHIGHAR  V+FD+LYRYLRHLGY+
Sbjct: 10  DLTLYNTMTQLKEVYKPMNPGKIGIYVCGITAYDYSHIGHARAAVSFDLLYRYLRHLGYQ 69

Query: 134 VLYVRNFTDVDDKIIARANELGEDPLNLSKRYCEEFRQDMVYLHCLPPSVEPRVSDHMPQ 193
           V YVRNFTDVDDK    A   GE PL+LS R+CEE+  DM  L CL P+ +PRVSDHM Q
Sbjct: 70  VTYVRNFTDVDDK----AKNCGEKPLDLSNRFCEEYLLDMAALQCLLPTHQPRVSDHMEQ 129

Query: 194 IVDMIKQILDNGYAYSVDGDVYFNVDKFPEYGLLSGRKLEDNRAGERVAVDSRKKNPADF 253
           I+ MI++I++NG  Y+V GDV+F+VDK P YG LSG++L+  +AG+RVAVDSRK+NPADF
Sbjct: 130 IIKMIEKIIENGCGYAVGGDVFFSVDKSPSYGQLSGQRLDHTQAGKRVAVDSRKRNPADF 189

Query: 254 ALWKSAKEGEPFWESPWGPGRPGWHIECSAMSATYLGYSFDIHGGGMDLVFPHHENEIAQ 313
           AL K+AK GEP WESPWG GRPGWHIE            FDIHGGG DL FPHHENEIAQ
Sbjct: 190 ALRKAAKSGEPSWESPWGHGRPGWHIE------------FDIHGGGADLKFPHHENEIAQ 249

Query: 314 SCAACRTSNISYWVHNGFVTIDSEKMSKSLGNFFTIRQVIDLYHPLALRLFLLGTHYRSP 373
           +CAAC  S ++YW+HNG VT ++ KM KSL NFFTIRQ+   YHPLALR FL+   YRSP
Sbjct: 250 TCAACEDSGVNYWLHNGHVTNNNVKMGKSLNNFFTIRQIAANYHPLALRHFLMSAQYRSP 309

Query: 374 INYSDFLLESASDRIYYIYQTLEDCRTVISQQDESSFKGSIAPSLAEEINKFYNVFLTSM 433
           +NYS   LES+SD +Y +    E+    + +  +S+          E I K         
Sbjct: 310 LNYSVSQLESSSDALYSLSPYREEMSGDVGKTQQSA-------EAKEMIKK--------- 369

Query: 434 SDDIHTPVVLAAISDPLKIINDLLHTRKGKKQESRME---SLAALEKIIGSVLSILGLMP 493
                       + + LK IN  +   K  +++ RM    SL  +EK +  VL +LGL+ 
Sbjct: 370 ------------VKNALKFINVSISKLKKMQKKQRMSLVVSLVEVEKAVREVLDVLGLLT 429

Query: 494 A-SYSEALQELKEKALTRAKMTNDQVVEKIEERNAARKNKEYEKSDAIRTDLAAVGITLM 553
             SY E L+++K+KALTRA M  ++V+++IEERN ARK+K++ +SD IR  LA  GI L 
Sbjct: 430 TLSYGELLKDMKQKALTRAGMGEEEVLQRIEERNMARKSKDFRRSDRIRELLAFKGIFLE 459

Query: 554 DGPNGTTWRPAIPL 564
           D P  T WRP+ PL
Sbjct: 490 DVPGDTVWRPSTPL 459

BLAST of Lag0037223 vs. TAIR 10
Match: AT1G09620.1 (ATP binding;leucine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases )

HSP 1 Score: 45.1 bits (105), Expect = 2.3e-04
Identity = 37/133 (27.82%), Postives = 60/133 (45.11%), Query Frame = 0

Query: 291 YSFDIHGGGMDLVFPHHENEIAQSCAACRTSNISYWVH-NGFVTIDSEKMSKSLGNFFTI 350
           Y  D+   G DL+  H    I    A     N    +  NG + ++SEKMSKS GNF T+
Sbjct: 667 YPLDLRVSGKDLIQNHLTFFIYNHTALMANRNWPRGIRCNGHIMLNSEKMSKSTGNFRTL 726

Query: 351 RQVIDLYHPLALRLFLLGTHYRSPINYSDFLLESASDRIYYIYQTLEDCRTVISQQDESS 410
           RQ I+ +     R  L        ++ ++F  E+A+  I  + + L     V+    ESS
Sbjct: 727 RQSIEEFSATGTRFCLADA--GDGVDDANFAFETANAAILRLTKELTWMEEVLDV--ESS 786

Query: 411 FKGSIAPSLAEEI 423
            +     + A+++
Sbjct: 787 LRTGPPSTYADKV 795

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023524130.11.8e-30691.81cysteine--tRNA ligase, chloroplastic/mitochondrial-like isoform X2 [Cucurbita pe... [more]
XP_038900128.15.9e-30591.15cysteine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Benincasa hispida... [more]
KAG6591556.11.7e-30490.91Cysteine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrospe... [more]
KAG7024444.18.5e-30491.05Cysteine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita argyrosperma subsp... [more]
XP_008466439.11.4e-30390.28PREDICTED: cysteine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucumi... [more]
Match NameE-valueIdentityDescription
F4IPY23.9e-23571.96Cysteine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=37... [more]
Q0IZQ25.3e-20871.26Cysteine--tRNA ligase CPS1 homolog, chloroplastic/mitochondrial OS=Oryza sativa ... [more]
A0A1D6LAG94.5e-20770.26Cysteine--tRNA ligase CPS1, chloroplastic/mitochondrial OS=Zea mays OX=4577 GN=C... [more]
B3LFA41.5e-15456.06Cysteine--tRNA ligase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At5g3883... [more]
Q9LYL33.1e-13952.83Cysteine--tRNA ligase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g5630... [more]
Match NameE-valueIdentityDescription
A0A5D3E6A87.0e-30490.28Cysteinyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3CSJ77.0e-30490.28Cysteinyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503847 PE=3 SV=1[more]
A0A5A7VBP99.2e-30490.28Cysteinyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
A0A6J1IMQ61.2e-30390.35Cysteinyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111476622 PE=3 SV=1[more]
A0A6J1C8721.2e-30390.45Cysteinyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111008869 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT2G31170.12.8e-23671.96Cysteinyl-tRNA synthetase, class Ia family protein [more]
AT2G31170.21.3e-18076.71Cysteinyl-tRNA synthetase, class Ia family protein [more]
AT5G38830.11.1e-15556.06Cysteinyl-tRNA synthetase, class Ia family protein [more]
AT3G56300.12.2e-14052.83Cysteinyl-tRNA synthetase, class Ia family protein [more]
AT1G09620.12.3e-0427.82ATP binding;leucine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP b... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR024909Cysteinyl-tRNA synthetase/mycothiol ligasePRINTSPR00983TRNASYNTHCYScoord: 98..109
score: 60.98
coord: 263..281
score: 77.75
coord: 134..143
score: 62.27
coord: 294..315
score: 75.62
IPR024909Cysteinyl-tRNA synthetase/mycothiol ligasePANTHERPTHR10890CYSTEINYL-TRNA SYNTHETASEcoord: 74..570
IPR032678tRNA synthetases class I, catalytic domainPFAMPF01406tRNA-synt_1ecoord: 88..384
e-value: 1.7E-127
score: 425.0
IPR032678tRNA synthetases class I, catalytic domainCDDcd00672CysRS_corecoord: 76..377
e-value: 1.741E-114
score: 337.244
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 59..377
e-value: 3.9E-143
score: 478.1
IPR015803Cysteine-tRNA ligaseTIGRFAMTIGR00435TIGR00435coord: 76..558
e-value: 1.1E-162
score: 540.3
IPR015803Cysteine-tRNA ligaseHAMAPMF_00041Cys_tRNA_synthcoord: 75..560
score: 36.81776
NoneNo IPR availableGENE3D1.20.120.1910coord: 379..559
e-value: 1.8E-32
score: 114.6
NoneNo IPR availablePANTHERPTHR10890:SF25CYSTEINE--TRNA LIGASE, CHLOROPLASTIC/MITOCHONDRIALcoord: 74..570
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 74..384
IPR009080Aminoacyl-tRNA synthetase, class Ia, anticodon-bindingSUPERFAMILY47323Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetasescoord: 415..558

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0037223.1Lag0037223.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042407 cristae formation
biological_process GO:0006423 cysteinyl-tRNA aminoacylation
biological_process GO:0010197 polar nucleus fusion
biological_process GO:0043067 regulation of programmed cell death
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0009507 chloroplast
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005524 ATP binding
molecular_function GO:0004817 cysteine-tRNA ligase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding