Lag0037058 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0037058
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Cytochrome P450
Location	chr2: 3072198 .. 3072770 (+)
RNA-Seq Expression	Lag0037058
Synteny	Lag0037058

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGTCGATCTTCCGCCTGGAAACATTGGCTAGTCGTTTCTCGGCGAGACCGTCGGCTGTCTCTAGCCCTACTCCGCCACCACCATCGGAGATTTCATGGAGAATCACATCAAGAGGTAAAAACCCCCACATTAAACGCTTGACTCTCTGTTTTTTTATTCTGTTTTCATTTAGTTGTTTTGGAGATCGAATCTTTCGCATACAGATATGGCAAAGTGTACAAGTCGAATCTGTTCGGAGAGCCGACGGTGGTATCGGCCGACGCCGGACTCAACCGGTACATACTAAGCTTGTTGTTCACCGGCCACGAGACGTCGTCTGTTGCCATAGCTCTGGCCATCTTCTTCCTCTCCAGCAGCCAGGTCGCCGTCCAACAGTTGACGGTAAGTCACTAGAAAAGATTTCCGAAGCCAATTTCCCATTTTTCTTCCTATTCTCTGATTTCTCAGAGTTCGAAATACAGGAAGAACACTTGGAGATTGCTAGAGCCAACAAGCGATTAGGAAAGATGAAATTGGATTGGGAAGATTACAAGAAAATCGAATTCACACAAGGTGTAATGAATAATTGA

mRNA sequence

ATGTCGTCGATCTTCCGCCTGGAAACATTGGCTAGTCGTTTCTCGGCGAGACCGTCGGCTGTCTCTAGCCCTACTCCGCCACCACCATCGGAGATTTCATGGAGAATCACATCAAGAGTTGTTTTGGAGATCGAATCTTTCGCATACAGATATGGCAAAGTGTACAAGTCGAATCTGTTCGGAGAGCCGACGGTGGTATCGGCCGACGCCGGACTCAACCGGTACATACTAAGCTTGTTGTTCACCGGCCACGAGACGTCGTCTGTTGCCATAGCTCTGGCCATCTTCTTCCTCTCCAGCAGCCAGGTCGCCGTCCAACAGTTGACGGAAGAACACTTGGAGATTGCTAGAGCCAACAAGCGATTAGGAAAGATGAAATTGGATTGGGAAGATTACAAGAAAATCGAATTCACACAAGGTGTAATGAATAATTGA

Coding sequence (CDS)

ATGTCGTCGATCTTCCGCCTGGAAACATTGGCTAGTCGTTTCTCGGCGAGACCGTCGGCTGTCTCTAGCCCTACTCCGCCACCACCATCGGAGATTTCATGGAGAATCACATCAAGAGTTGTTTTGGAGATCGAATCTTTCGCATACAGATATGGCAAAGTGTACAAGTCGAATCTGTTCGGAGAGCCGACGGTGGTATCGGCCGACGCCGGACTCAACCGGTACATACTAAGCTTGTTGTTCACCGGCCACGAGACGTCGTCTGTTGCCATAGCTCTGGCCATCTTCTTCCTCTCCAGCAGCCAGGTCGCCGTCCAACAGTTGACGGAAGAACACTTGGAGATTGCTAGAGCCAACAAGCGATTAGGAAAGATGAAATTGGATTGGGAAGATTACAAGAAAATCGAATTCACACAAGGTGTAATGAATAATTGA

Protein sequence

MSSIFRLETLASRFSARPSAVSSPTPPPPSEISWRITSRVVLEIESFAYRYGKVYKSNLFGEPTVVSADAGLNRYILSLLFTGHETSSVAIALAIFFLSSSQVAVQQLTEEHLEIARANKRLGKMKLDWEDYKKIEFTQGVMNN

Homology

[Close]

BLAST of Lag0037058 vs. NCBI nr
Match: KAA0044749.1 (cytochrome P450 90B1 [Cucumis melo var. makuwa] >TYK16713.1 cytochrome P450 90B1 [Cucumis melo var. makuwa])

HSP 1 Score: 109.8 bits (273), Expect = 2.0e-20
Identity = 57/68 (83.82%), Postives = 59/68 (86.76%), Query Frame = 0


Query: 76  ILSLLFTGHETSSVAIALAIFFLSSSQVAVQQLTEEHLEIARANKRLGKMKLDWEDYKKI 135
           ILSLLF GHETSSVAIALAIFFLS S  AVQQLTEEHLEIAR  KRLGK  LDWEDYKK+
Sbjct: 305 ILSLLFAGHETSSVAIALAIFFLSGSPAAVQQLTEEHLEIARGKKRLGKTDLDWEDYKKM 364

Query: 136 EFTQGVMN 144
           EFTQ V+N
Sbjct: 365 EFTQSVIN 372

[Close]

BLAST of Lag0037058 vs. NCBI nr
Match: KAA0044749.1 (cytochrome P450 90B1 [Cucumis melo var. makuwa] >TYK16713.1 cytochrome P450 90B1 [Cucumis melo var. makuwa])

HSP 1 Score: 62.0 bits (149), Expect = 4.8e-06
Identity = 29/34 (85.29%), Postives = 31/34 (91.18%), Query Frame = 0


Query: 44 IESFAYRYGKVYKSNLFGEPTVVSADAGLNRYIL 78
          +E+   RYGKVYKSNLFGEPTVVSADAGLNRYIL
Sbjct: 64 MENHIRRYGKVYKSNLFGEPTVVSADAGLNRYIL 97

HSP 2 Score: 109.8 bits (273), Expect = 2.0e-20
Identity = 57/68 (83.82%), Postives = 59/68 (86.76%), Query Frame = 0


Query: 76  ILSLLFTGHETSSVAIALAIFFLSSSQVAVQQLTEEHLEIARANKRLGKMKLDWEDYKKI 135
           ILSLLF GHETSSVAIALAIFFLS S  AVQQLTEEHLEIAR  KRLGK  LDWEDYKK+
Sbjct: 280 ILSLLFAGHETSSVAIALAIFFLSGSPAAVQQLTEEHLEIARGKKRLGKTDLDWEDYKKM 339

Query: 136 EFTQGVMN 144
           EFTQ V+N
Sbjct: 340 EFTQSVIN 347

[Close]

BLAST of Lag0037058 vs. NCBI nr
Match: XP_008453737.1 (PREDICTED: cytochrome P450 90B1 [Cucumis melo])

HSP 1 Score: 62.0 bits (149), Expect = 4.8e-06
Identity = 29/34 (85.29%), Postives = 31/34 (91.18%), Query Frame = 0


Query: 44 IESFAYRYGKVYKSNLFGEPTVVSADAGLNRYIL 78
          +E+   RYGKVYKSNLFGEPTVVSADAGLNRYIL
Sbjct: 64 MENHIRRYGKVYKSNLFGEPTVVSADAGLNRYIL 97

HSP 2 Score: 109.8 bits (273), Expect = 2.0e-20
Identity = 57/68 (83.82%), Postives = 60/68 (88.24%), Query Frame = 0


Query: 76  ILSLLFTGHETSSVAIALAIFFLSSSQVAVQQLTEEHLEIARANKRLGKMKLDWEDYKKI 135
           ILSLLF GHETSSVAIALAIFFLS S  AVQQLTEEHL IAR NKRLGK +LDWEDYKK+
Sbjct: 280 ILSLLFAGHETSSVAIALAIFFLSGSSAAVQQLTEEHLGIARDNKRLGKTELDWEDYKKM 339

Query: 136 EFTQGVMN 144
           EFTQ V+N
Sbjct: 340 EFTQNVIN 347

[Close]

BLAST of Lag0037058 vs. NCBI nr
Match: XP_038877545.1 (cytochrome P450 90B1 [Benincasa hispida])

HSP 1 Score: 58.9 bits (141), Expect = 4.1e-05
Identity = 28/34 (82.35%), Postives = 30/34 (88.24%), Query Frame = 0


Query: 44 IESFAYRYGKVYKSNLFGEPTVVSADAGLNRYIL 78
          +E+   RYGKVYKSNLFGE TVVSADAGLNRYIL
Sbjct: 64 MENHIKRYGKVYKSNLFGETTVVSADAGLNRYIL 97

HSP 2 Score: 109.8 bits (273), Expect = 2.0e-20
Identity = 56/68 (82.35%), Postives = 61/68 (89.71%), Query Frame = 0


Query: 76  ILSLLFTGHETSSVAIALAIFFLSSSQVAVQQLTEEHLEIARANKRLGKMKLDWEDYKKI 135
           ILSLLF GHETSSV+IALAIFFLS S+  VQQLTEEHLEIAR NKRLGK +LDWEDYKK+
Sbjct: 278 ILSLLFAGHETSSVSIALAIFFLSGSRATVQQLTEEHLEIARGNKRLGKAELDWEDYKKM 337

Query: 136 EFTQGVMN 144
           EFTQ V+N
Sbjct: 338 EFTQCVIN 345

[Close]

BLAST of Lag0037058 vs. NCBI nr
Match: XP_022941684.1 (cytochrome P450 90B1 [Cucurbita moschata])

HSP 1 Score: 61.2 bits (147), Expect = 8.3e-06
Identity = 32/62 (51.61%), Postives = 36/62 (58.06%), Query Frame = 0


Query: 28 PPSEISWRITSRVVLEIESFA------------YRYGKVYKSNLFGEPTVVSADAGLNRY 78
          PP  + W      V  +  ++             RYGKVYKSNLFGEPTVVSADAGLNRY
Sbjct: 34 PPGNMGWPFLGETVGYLRPYSATTIGDFMKNHITRYGKVYKSNLFGEPTVVSADAGLNRY 93

HSP 2 Score: 109.0 bits (271), Expect = 3.5e-20
Identity = 56/68 (82.35%), Postives = 59/68 (86.76%), Query Frame = 0


Query: 76  ILSLLFTGHETSSVAIALAIFFLSSSQVAVQQLTEEHLEIARANKRLGKMKLDWEDYKKI 135
           ILSLLF GHETSSVAIALAIFFLS S  AVQQLTEEHLEI R  KRLGK +LDWEDYKK+
Sbjct: 280 ILSLLFAGHETSSVAIALAIFFLSGSPAAVQQLTEEHLEITRGKKRLGKTELDWEDYKKM 339

Query: 136 EFTQGVMN 144
           EFTQ V+N
Sbjct: 340 EFTQSVIN 347

[Close]

BLAST of Lag0037058 vs. ExPASy Swiss-Prot
Match: O64989 (Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2)

HSP 1 Score: 94.7 bits (234), Expect = 8.8e-19
Identity = 48/68 (70.59%), Postives = 58/68 (85.29%), Query Frame = 0


Query: 76  ILSLLFTGHETSSVAIALAIFFLSSSQVAVQQLTEEHLEIARANKRLGKMKLDWEDYKKI 135
           ILSLLF GHETSSVAIALAIFFL +   AV++L EEHLEIARA K LG+ +L+W+DYKK+
Sbjct: 305 ILSLLFAGHETSSVAIALAIFFLQACPKAVEELREEHLEIARAKKELGESELNWDDYKKM 364

Query: 136 EFTQGVMN 144
           +FTQ V+N
Sbjct: 365 DFTQCVIN 372

HSP 2 Score: 56.2 bits (134), Expect = 3.5e-07
Identity = 23/28 (82.14%), Postives = 28/28 (100.00%), Query Frame = 0


Query: 50  RYGKVYKSNLFGEPTVVSADAGLNRYIL 78
           +YGK+Y+SNLFGEPT+VSADAGLNR+IL
Sbjct: 74  KYGKIYRSNLFGEPTIVSADAGLNRFIL 101

[Close]

BLAST of Lag0037058 vs. ExPASy Swiss-Prot
Match: Q5CCK3 (Cytochrome P450 90B2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90B2 PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 2.7e-15
Identity = 43/68 (63.24%), Postives = 52/68 (76.47%), Query Frame = 0


Query: 76  ILSLLFTGHETSSVAIALAIFFLSSSQVAVQQLTEEHLEIARANKRLGKMKLDWEDYKKI 135
           +LSLLF GHETSS+A+ALAIFFL     AVQ+L EEHL IAR  +  G+ KL WEDYK++
Sbjct: 299 LLSLLFAGHETSSMALALAIFFLEGCPKAVQELREEHLGIARRQRLRGECKLSWEDYKEM 358

Query: 136 EFTQGVMN 144
            FTQ V+N
Sbjct: 359 VFTQCVIN 366

HSP 2 Score: 54.7 bits (130), Expect = 1.0e-06
Identity = 25/34 (73.53%), Postives = 29/34 (85.29%), Query Frame = 0


Query: 44  IESFAYRYGKVYKSNLFGEPTVVSADAGLNRYIL 78
           +E    RYGK+Y+S+LFGE TVVSADAGLNRYIL
Sbjct: 83  MEQHIARYGKIYRSSLFGERTVVSADAGLNRYIL 116

[Close]

BLAST of Lag0037058 vs. ExPASy Swiss-Prot
Match: Q50EK6 (Abietadienol/abietadienal oxidase OS=Pinus taeda OX=3352 GN=CYP720B1 PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 5.7e-10
Identity = 43/113 (38.05%), Postives = 65/113 (57.52%), Query Frame = 0


Query: 31  EISWRITSRVVLEIESFAYRYGKVYKSNLFGEPTVVSADAGLNRYILSLLFTGHETSSVA 90
           E+  R  S ++ E     +   K   + L  E ++  +D  +  +IL LLF GHETSS A
Sbjct: 242 ELLIRKISNMIKERRDHPHIVHKDLLTKLLEEDSI--SDEIICDFILFLLFAGHETSSRA 301

Query: 91  IALAIFFLSSSQVAVQQLTEEHLEIARANKRLGKMKLDWEDYKKIEFTQGVMN 144
           +  AI FL++   A+ Q+ EEH  I +A  + G  KL+W+DYK ++FTQ V+N
Sbjct: 302 MTFAIKFLTTCPKALTQMKEEHDAILKA--KGGHKKLEWDDYKSMKFTQCVIN 350

[Close]

BLAST of Lag0037058 vs. ExPASy Swiss-Prot
Match: Q50EK5 (Cytochrome P450 720B2 OS=Pinus taeda OX=3352 GN=CYP720B2 PE=2 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 3.2e-08
Identity = 33/91 (36.26%), Postives = 53/91 (58.24%), Query Frame = 0


Query: 53  KVYKSNLFGEPTVVSADAGLNRYILSLLFTGHETSSVAIALAIFFLSSSQVAVQQLTEEH 112
           K+ K + F E  +         +IL LLF GHETSS +++ AI FL+    A+++L  EH
Sbjct: 269 KLLKEDSFSEEIIAD-------FILFLLFAGHETSSRSMSFAIKFLTDCPRALEELKAEH 328

Query: 113 LEIARANKRLGKMKLDWEDYKKIEFTQGVMN 144
             + +    L   KL+W+DY+ ++FTQ V++
Sbjct: 329 DALLKRKGNLKNQKLNWDDYQSLKFTQCVIH 352

[Close]

BLAST of Lag0037058 vs. ExPASy Swiss-Prot
Match: Q2RAP4 (Cytochrome P450 90A3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90A3 PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 1.7e-06
Identity = 29/69 (42.03%), Postives = 43/69 (62.32%), Query Frame = 0


Query: 75  YILSLLFTGHETSSVAIALAIFFLSSSQVAVQQLTEEHLEIARANKRLGKMKLDWEDYKK 134
           + LSLL  G+ET+S+ + LA+ FL+ +  A+ +L EEH  I     +  K  L+W DYK 
Sbjct: 297 FCLSLLVAGYETTSMLMTLAVKFLTETPAALAELKEEHANIRDMKGK--KQPLEWSDYKS 356

Query: 135 IEFTQGVMN 144
           + FTQ V+N
Sbjct: 357 MPFTQCVIN 363

The following BLAST results are available for this feature:

BLAST of Lag0037058 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0044749.1	2.0e-20	83.82	cytochrome P450 90B1 [Cucumis melo var. makuwa] >TYK16713.1 cytochrome P450 90B1...	[more]
KAA0044749.1	4.8e-06	85.29	cytochrome P450 90B1 [Cucumis melo var. makuwa] >TYK16713.1 cytochrome P450 90B1...	[more]
XP_008453737.1	4.8e-06	85.29	PREDICTED: cytochrome P450 90B1 [Cucumis melo]	[more]
XP_038877545.1	4.1e-05	82.35	cytochrome P450 90B1 [Benincasa hispida]	[more]
XP_022941684.1	8.3e-06	51.61	cytochrome P450 90B1 [Cucurbita moschata]	[more]

Pages

BLAST of Lag0037058 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O64989	8.8e-19	70.59	Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2	[more]
Q5CCK3	2.7e-15	63.24	Cytochrome P450 90B2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90B2 PE=1 SV...	[more]
Q50EK6	5.7e-10	38.05	Abietadienol/abietadienal oxidase OS=Pinus taeda OX=3352 GN=CYP720B1 PE=1 SV=1	[more]
Q50EK5	3.2e-08	36.26	Cytochrome P450 720B2 OS=Pinus taeda OX=3352 GN=CYP720B2 PE=2 SV=1	[more]
Q2RAP4	1.7e-06	42.03	Cytochrome P450 90A3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90A3 PE=2 SV...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 68..143 e-value: 1.9E-7 score: 32.4
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 75..143
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 72..142 e-value: 9.7E-6 score: 24.6
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 76..143
None	No IPR available	PANTHER	PTHR24286:SF194	CYTOCHROME P450 90B1	coord: 46..78 coord: 76..143
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 46..78

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0037058.1	Lag0037058.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0010268	brassinosteroid homeostasis
biological_process	GO:0048366	leaf development
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0080132	fatty acid alpha-hydroxylase activity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen