Lag0037027 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0037027 vs. NCBI nr
Match: XP_023535224.1 (activating signal cointegrator 1 complex subunit 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1352.4 bits (3499), Expect = 0.0e+00
Identity = 724/853 (84.88%), Postives = 758/853 (88.86%), Query Frame = 0

Query: 1   MSNRYNQDG-NKGLRKDQKKYVPKNLNQSTRELPNPKPTLSTSLRQSLPKPSDSAAASTT 60
           MSNRYN DG NKGLRK+QKKY+PKN  QST E PNPKP+LSTSLRQSLPKPSDSAA S++
Sbjct: 1   MSNRYNHDGNNKGLRKEQKKYIPKNQIQSTNERPNPKPSLSTSLRQSLPKPSDSAAVSSS 60

Query: 61  AAPSMSRIHMGANGDWVSSRGSGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 120
           A PSMSRI MGANGDWVSSR +GGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN
Sbjct: 61  ATPSMSRIQMGANGDWVSSRATGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 120

Query: 121 RELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAGVIVGEFEL 180
           RELSRLLKLSAKEFWTEVA DTSLHEFLDSFLKFRSRWYDFP+RGANGIVAGVIVGE EL
Sbjct: 121 RELSRLLKLSAKEFWTEVARDTSLHEFLDSFLKFRSRWYDFPHRGANGIVAGVIVGECEL 180

Query: 181 SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLT 240
           SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIY HENEDLT
Sbjct: 181 SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYFHENEDLT 240

Query: 241 RILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGENGYSKLQAD 300
           R LVENAIKSQPSIHE LPSVISHFL+IVSMMHQRCNSSLETLFSS S+GE+GYSKLQAD
Sbjct: 241 RTLVENAIKSQPSIHETLPSVISHFLSIVSMMHQRCNSSLETLFSSGSNGESGYSKLQAD 300

Query: 301 FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 360
           FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG
Sbjct: 301 FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 360

Query: 361 FHIVFVSRGDEMMSNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVPVSMKMFPA 420
           F IVFV RGD+MMS+V TSLKMLALR+VSLGWKLLEICYLGDEVFGNDL VPVSMKMFPA
Sbjct: 361 FQIVFVPRGDDMMSSVVTSLKMLALRVVSLGWKLLEICYLGDEVFGNDLAVPVSMKMFPA 420

Query: 421 NVEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSIINRINSLRNNGWILI 480
           NVEDPVIRADI IQTLREINGISQQ PDKQLGQTFLQRMEKNHSI+NRINSLRNNGWI +
Sbjct: 421 NVEDPVIRADIFIQTLREINGISQQAPDKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFV 480

Query: 481 DDEQFDYLSTVVMYTSTSNIKDPSLSKAPAMSHISEVDEDAAMLESKICQIKDLFPEYGS 540
           DDEQFDY+ST+V YT T NIKD SLSKAP MSHISEVDED+AMLESKICQIKDLFPEYGS
Sbjct: 481 DDEQFDYISTIV-YTPTPNIKDSSLSKAPVMSHISEVDEDSAMLESKICQIKDLFPEYGS 540

Query: 541 GFLAACLVAYNQNPEEVIQRILEGTLHSDLQSLDTSLETMPVPNSSATANNRNDKGKGKL 600
           GFLAACLVAYNQNPEEVIQRILEGTLH+DLQSLDTSLETMPVPNSSA A NRNDKGKGKL
Sbjct: 541 GFLAACLVAYNQNPEEVIQRILEGTLHADLQSLDTSLETMPVPNSSAAAINRNDKGKGKL 600

Query: 601 IESPTVAYTHQVSQGKDFPIEGPSVSSTSGGRYVRKSKDDMPYSETLDNRNEADPLRTAA 660
            ES TVAYT QVSQ KD  +EGPSVSST GGRYVRKSKDDMPYSETLD+RNEAD +RTAA
Sbjct: 601 FESSTVAYTDQVSQSKDLMVEGPSVSSTFGGRYVRKSKDDMPYSETLDSRNEADSVRTAA 660

Query: 661 LISQYEYEDEYDDSFDDLGISVAETATEDNEDLVDQKLSSGLGNSSNSTNASSAQNAPSS 720
           L+SQYEYEDEYDDSFDDLGIS+AETATEDNEDLV QKLSS LGNSSNS NASSAQNA +S
Sbjct: 661 LVSQYEYEDEYDDSFDDLGISIAETATEDNEDLVGQKLSSDLGNSSNSKNASSAQNASNS 720

Query: 721 KWGSRRKPQYYVKD-----------------ERTNIWTWTRRQ----------LALGAVK 780
           KWGS+RKPQYYVKD                 +  ++ T  +++          L LGAVK
Sbjct: 721 KWGSKRKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQAQKELIHGLGRGGNLPLGAVK 780

Query: 781 KLTESEQDSQPDVSTVDPRENGRKSWGRG-RREGGSGTAVGVPAAPEGQGKQSNVAEVQT 825
           KLTESEQDSQPDVS  DPREN RKSWGRG RR+ GSG+A GV   PEGQGKQ NVAEV  
Sbjct: 781 KLTESEQDSQPDVSAADPRENVRKSWGRGSRRDLGSGSAAGV---PEGQGKQPNVAEVSE 840

BLAST of Lag0037027 vs. NCBI nr
Match: KAG6591418.1 (Transcription factor basic helix-loop-helix 85, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1346.6 bits (3484), Expect = 0.0e+00
Identity = 721/853 (84.53%), Postives = 755/853 (88.51%), Query Frame = 0

Query: 1    MSNRYNQDG-NKGLRKDQKKYVPKNLNQSTRELPNPKPTLSTSLRQSLPKPSDSAAASTT 60
            MSNRYN DG NKGLRK+QKKY+PKN  QST E PNPKP LSTSLRQSLPKPSDSAA S++
Sbjct: 391  MSNRYNHDGNNKGLRKEQKKYIPKNQIQSTNERPNPKPNLSTSLRQSLPKPSDSAAVSSS 450

Query: 61   AAPSMSRIHMGANGDWVSSRGSGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 120
            A PSMSRI MGANGDWVSSR +GGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN
Sbjct: 451  ATPSMSRIQMGANGDWVSSRATGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 510

Query: 121  RELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAGVIVGEFEL 180
            RELSRLLKLSAKEFWTEVA DTSLHEFLDSFLKFRSRWYDFP+RGANGIVAGVIVGE EL
Sbjct: 511  RELSRLLKLSAKEFWTEVARDTSLHEFLDSFLKFRSRWYDFPHRGANGIVAGVIVGECEL 570

Query: 181  SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLT 240
            SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIY HENEDLT
Sbjct: 571  SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYYHENEDLT 630

Query: 241  RILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGENGYSKLQAD 300
            R LVENAIKSQPSIHE LPSVISHFL+IVSMMHQRCNSSLETLFSSS +GE+GYSKLQAD
Sbjct: 631  RTLVENAIKSQPSIHETLPSVISHFLSIVSMMHQRCNSSLETLFSSSRNGESGYSKLQAD 690

Query: 301  FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 360
             LEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG
Sbjct: 691  LLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 750

Query: 361  FHIVFVSRGDEMMSNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVPVSMKMFPA 420
            F IVFV RGD+MMS+V TS KMLALR+VSLGWKLLEICYLGDEVFGNDL VPVSMKMFPA
Sbjct: 751  FQIVFVPRGDDMMSSVVTSSKMLALRVVSLGWKLLEICYLGDEVFGNDLAVPVSMKMFPA 810

Query: 421  NVEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSIINRINSLRNNGWILI 480
            NVEDPVIRADI IQTLREINGISQQ PDKQLGQTFLQRMEKNHSI+NRINSLRNNGWI +
Sbjct: 811  NVEDPVIRADIFIQTLREINGISQQAPDKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFV 870

Query: 481  DDEQFDYLSTVVMYTSTSNIKDPSLSKAPAMSHISEVDEDAAMLESKICQIKDLFPEYGS 540
            DDEQFDY+ST+V YT T NIKD SLSKAP MSHISEVDED+AMLESKICQIKDLFPEYGS
Sbjct: 871  DDEQFDYISTIV-YTPTPNIKDSSLSKAPVMSHISEVDEDSAMLESKICQIKDLFPEYGS 930

Query: 541  GFLAACLVAYNQNPEEVIQRILEGTLHSDLQSLDTSLETMPVPNSSATANNRNDKGKGKL 600
            GFLAACLVAYNQNPEEVIQRILEGTLH+DLQSLDTSLETMPVPNSSA A NRNDKGKGKL
Sbjct: 931  GFLAACLVAYNQNPEEVIQRILEGTLHADLQSLDTSLETMPVPNSSAAAINRNDKGKGKL 990

Query: 601  IESPTVAYTHQVSQGKDFPIEGPSVSSTSGGRYVRKSKDDMPYSETLDNRNEADPLRTAA 660
             ES TVAYT QVSQ KD  +EGPSVSST GGRYVRKSKDDMPYSETLD+RNEAD +RTAA
Sbjct: 991  FESSTVAYTDQVSQSKDLMVEGPSVSSTFGGRYVRKSKDDMPYSETLDSRNEADSVRTAA 1050

Query: 661  LISQYEYEDEYDDSFDDLGISVAETATEDNEDLVDQKLSSGLGNSSNSTNASSAQNAPSS 720
            L+SQYEYEDEYDDSFDDLGIS+AETATEDNEDLV QKLSS LGNSSNS NASSAQNA +S
Sbjct: 1051 LVSQYEYEDEYDDSFDDLGISIAETATEDNEDLVGQKLSSDLGNSSNSKNASSAQNASNS 1110

Query: 721  KWGSRRKPQYYVKD-----------------ERTNIWTWTRRQ----------LALGAVK 780
            KWGS+RKPQYYVKD                 +  ++ T  +++          L LGAVK
Sbjct: 1111 KWGSKRKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQAQKELIHGLGRGGNLPLGAVK 1170

Query: 781  KLTESEQDSQPDVSTVDPRENGRKSWGRG-RREGGSGTAVGVPAAPEGQGKQSNVAEVQT 825
            KLT+SEQDSQPDVS  DPR+N RKSWGRG RRE GSG+A GV   PEGQGKQ NVAEV  
Sbjct: 1171 KLTDSEQDSQPDVSAADPRDNVRKSWGRGSRREVGSGSAAGV---PEGQGKQPNVAEVSE 1230

BLAST of Lag0037027 vs. NCBI nr
Match: XP_022936905.1 (activating signal cointegrator 1 complex subunit 2-like [Cucurbita moschata])

HSP 1 Score: 1345.1 bits (3480), Expect = 0.0e+00
Identity = 721/855 (84.33%), Postives = 756/855 (88.42%), Query Frame = 0

Query: 1   MSNRYNQDG-NKGLRKDQKKYVPKNLNQSTRELPNPKPTLSTSLRQSLPKPSDSAAASTT 60
           MSNRYN DG NKGLRK+QKKY+PKN  QST E PNPKP LSTSLRQSLPKPSDSAA S++
Sbjct: 1   MSNRYNHDGNNKGLRKEQKKYIPKNQIQSTNERPNPKPNLSTSLRQSLPKPSDSAAVSSS 60

Query: 61  AAPSMSRIHMGANGDWVSSRGSGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 120
           A PSMSRI MGANGDWVSSR +GGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN
Sbjct: 61  ATPSMSRIQMGANGDWVSSRATGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 120

Query: 121 RELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAGVIVGEFEL 180
           RELSRLLKLSAKEFWTEVA DTSLHEFLDSFLKFRSRWYDFP+RGANGIVAGVIVGE EL
Sbjct: 121 RELSRLLKLSAKEFWTEVARDTSLHEFLDSFLKFRSRWYDFPHRGANGIVAGVIVGECEL 180

Query: 181 SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLT 240
           SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIY HENEDLT
Sbjct: 181 SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYYHENEDLT 240

Query: 241 RILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGENGYSKLQAD 300
           R LVENAIKSQPSIHE LPSVISHFL+IVSMMHQRCNSSLETLFSSSS GE+GYSKLQAD
Sbjct: 241 RTLVENAIKSQPSIHETLPSVISHFLSIVSMMHQRCNSSLETLFSSSSSGESGYSKLQAD 300

Query: 301 FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 360
           FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG
Sbjct: 301 FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 360

Query: 361 FHIVFVSRGDEMMSNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVPVSMKMFPA 420
           F IVFV RGD+MMS+V TSLKMLALR+VSLGWKLLEICYLGDEVFGNDL VPVSMKMFPA
Sbjct: 361 FQIVFVPRGDDMMSSVVTSLKMLALRVVSLGWKLLEICYLGDEVFGNDLAVPVSMKMFPA 420

Query: 421 NVEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSIINRINSLRNNGWILI 480
           NVEDPVIRADI IQTLREINGISQQ PDKQLGQTFLQRMEKNHS++NRINSLRNNGWI +
Sbjct: 421 NVEDPVIRADIFIQTLREINGISQQAPDKQLGQTFLQRMEKNHSVMNRINSLRNNGWIFV 480

Query: 481 DDEQFDYLSTVVMYTSTSNIKDPSLSKAPAMSHISEVDEDAAMLESKICQIKDLFPEYGS 540
           DDEQFDY+S +V YT T NIKD SLSKAP MSHISEVDED+AMLESKICQIKDLFPEYGS
Sbjct: 481 DDEQFDYISKIV-YTPTPNIKDSSLSKAPVMSHISEVDEDSAMLESKICQIKDLFPEYGS 540

Query: 541 GFLAACLVAYNQNPEEVIQRILEGTLHSDLQSLDTSLETMPVPNSSATANNRNDKGKGKL 600
           GFLAACLVAYNQNPEEVIQRILEGTLH+DLQSLDTSLETMPVPNSSA A NRNDKGKGKL
Sbjct: 541 GFLAACLVAYNQNPEEVIQRILEGTLHADLQSLDTSLETMPVPNSSAAAINRNDKGKGKL 600

Query: 601 IESPTVAYTHQVSQGKDFPIEGPSVSSTSGGRYVRKSKDDMPYSETLDNRNEADPLRTAA 660
            ES TVAYT QVSQ KD  +EGPSVSST  GRYVRKSKDDMPYSETLD+RNEAD +RTA+
Sbjct: 601 FESSTVAYTDQVSQSKDLMVEGPSVSSTFSGRYVRKSKDDMPYSETLDSRNEADSVRTAS 660

Query: 661 LISQYEYEDEYDDSFDDLGISVAETATEDNEDLVDQKLSSGLGNSSNSTNASSAQNAPSS 720
           L+SQYEYEDEYDDSFDDLGIS+AETATEDNEDLV QKLSS LGNSSNS NASSAQNA +S
Sbjct: 661 LVSQYEYEDEYDDSFDDLGISIAETATEDNEDLVSQKLSSDLGNSSNSKNASSAQNASNS 720

Query: 721 KWGSRRKPQYYVKD-----------------ERTNIWTWTRRQ----------LALGAVK 780
           KWGS+RKPQYYVKD                 +  ++ T  +++          L LGAVK
Sbjct: 721 KWGSKRKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQAQKELIHGLGRGGNLPLGAVK 780

Query: 781 KLTESEQDSQPDVSTVDPRENGRKSWGRG-RREGGSGTAVGVPAAPEGQGKQSNVAEVQT 827
           KLTESEQDSQPDVS  DPR+N RKSWGRG RRE GSG+A GV   PEGQGKQ NVAEV  
Sbjct: 781 KLTESEQDSQPDVSAADPRDNVRKSWGRGSRREVGSGSAAGV---PEGQGKQPNVAEV-- 840

BLAST of Lag0037027 vs. NCBI nr
Match: XP_038897592.1 (activating signal cointegrator 1 complex subunit 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 1343.6 bits (3476), Expect = 0.0e+00
Identity = 712/849 (83.86%), Postives = 752/849 (88.57%), Query Frame = 0

Query: 1   MSNRYNQDGNKGLRKDQKKYVPKNLNQSTRELPNPKPTLSTSLRQSLPKPSDSAAASTTA 60
           MSNRYN DGNKGLRKDQKKY+PKN NQST ELPNPKPTLS+SLRQSLPKPSDSAA +T+A
Sbjct: 1   MSNRYNHDGNKGLRKDQKKYIPKNQNQSTNELPNPKPTLSSSLRQSLPKPSDSAAVTTSA 60

Query: 61  APSMSRIHMGANGDWVSSRGSGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLNR 120
           APS SRI MGANGDWVSSR SGGSFVNYLPQDEAVATGLRAE GALDPMESQRVVDLLNR
Sbjct: 61  APSTSRIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEAGALDPMESQRVVDLLNR 120

Query: 121 ELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAGVIVGEFELS 180
           ELSRLLKLSAKEFW EVA DTSLHEFLDSFLKFRSRWYDFP+RGANGIVAGVIVGE+ELS
Sbjct: 121 ELSRLLKLSAKEFWREVAMDTSLHEFLDSFLKFRSRWYDFPHRGANGIVAGVIVGEYELS 180

Query: 181 RRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLTR 240
           RRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIY+HENEDLTR
Sbjct: 181 RRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYSHENEDLTR 240

Query: 241 ILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGENGYSKLQADF 300
           ILVENAIKSQPSIHE LPSVISHFL+IVSMMH+RC+SSLETLFSSS+HG +GYSKLQADF
Sbjct: 241 ILVENAIKSQPSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSNHGGSGYSKLQADF 300

Query: 301 LEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQGF 360
           LEVIDFINDAIVTLDSFV AYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLP L +GF
Sbjct: 301 LEVIDFINDAIVTLDSFVTAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPYLHKGF 360

Query: 361 HIVFVSRGDEMMSNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVPVSMKMFPAN 420
            IV V +GDEM+SNVATSLKMLALRIVSLGWKLLEICYL DEVFG+ LPVPVSMKMFPAN
Sbjct: 361 QIVLVPQGDEMLSNVATSLKMLALRIVSLGWKLLEICYLDDEVFGHALPVPVSMKMFPAN 420

Query: 421 VEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSIINRINSLRNNGWILID 480
           VEDPVIRADILIQTLREINGISQQ  DKQLGQTFLQRMEKNHS++NRINSLRNNGWI +D
Sbjct: 421 VEDPVIRADILIQTLREINGISQQASDKQLGQTFLQRMEKNHSVMNRINSLRNNGWIFVD 480

Query: 481 DEQFDYLSTVVMYTSTSNIKDPSLSKAPAMSHISEVDEDAAMLESKICQIKDLFPEYGSG 540
           DEQFDYLST+VMYT TS++K+PSLSKAP MSHISEVDEDAAMLESKICQIKDLFPEYGSG
Sbjct: 481 DEQFDYLSTIVMYTPTSDVKNPSLSKAPVMSHISEVDEDAAMLESKICQIKDLFPEYGSG 540

Query: 541 FLAACLVAYNQNPEEVIQRILEGTLHSDLQSLDTSLETMPVPNSSATANNRNDKGKGKLI 600
           FLAACL AYNQNPEEVI+RILEGTLHSDLQSLDTSLET PVPNS ATANNRNDKGKGKL 
Sbjct: 541 FLAACLEAYNQNPEEVIERILEGTLHSDLQSLDTSLETKPVPNSRATANNRNDKGKGKLF 600

Query: 601 ESPTVAYTHQVSQGKDFPIEGPSVSSTSGGRYVRKSKDDMPYSETLDNRNEADPLRTAAL 660
           E PTVAY  QVS+ KD   EGPSVSSTS GR+VRKSKD+MPYSETLDNRNEADP+RTAAL
Sbjct: 601 EFPTVAYIDQVSRDKDLTSEGPSVSSTSVGRFVRKSKDNMPYSETLDNRNEADPVRTAAL 660

Query: 661 ISQYEYEDEYDDSFDDLGISVAETATEDNEDLVDQKLSSGLGNSSNSTNASSAQNAPSSK 720
           ISQYEYEDEYDDSFDDLGIS+AET TEDNED + QKLSS  G+SSNSTN SSA NAP+SK
Sbjct: 661 ISQYEYEDEYDDSFDDLGISIAETTTEDNEDSIGQKLSSNSGSSSNSTNGSSASNAPNSK 720

Query: 721 WGSRRKPQYYVKD-----------------ERTNIWTWTRRQ----------LALGAVKK 780
           WGSRRKPQYYVKD                 +  ++ T  +++          L LGAVKK
Sbjct: 721 WGSRRKPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKK 780

Query: 781 LTESEQDSQPDVSTVDPRENG-RKSWGRGRREGGSGTAVGVPAAPEGQGKQSNVAEVQTG 822
           LTE+EQDSQPDVS  DPR+N  RKSWGRGRRE  SGTA   P  PEGQGKQ NVAEV   
Sbjct: 781 LTEAEQDSQPDVSAGDPRDNNVRKSWGRGRRERESGTA---PGMPEGQGKQPNVAEVSDR 840

BLAST of Lag0037027 vs. NCBI nr
Match: XP_022976975.1 (activating signal cointegrator 1 complex subunit 2-like [Cucurbita maxima])

HSP 1 Score: 1341.3 bits (3470), Expect = 0.0e+00
Identity = 717/853 (84.06%), Postives = 753/853 (88.28%), Query Frame = 0

Query: 1   MSNRYNQDG-NKGLRKDQKKYVPKNLNQSTRELPNPKPTLSTSLRQSLPKPSDSAAASTT 60
           MSNRYN DG NKG RK+QKKY+PKN  QST E PNPKP LSTSLRQSLPKPSDSAA S++
Sbjct: 1   MSNRYNHDGSNKGRRKEQKKYIPKNQIQSTNERPNPKPNLSTSLRQSLPKPSDSAAVSSS 60

Query: 61  AAPSMSRIHMGANGDWVSSRGSGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 120
           A PSMSRI MGANGDWVSSR +GGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN
Sbjct: 61  ATPSMSRIQMGANGDWVSSRATGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 120

Query: 121 RELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAGVIVGEFEL 180
           RELSRLLKLSA+EFWTEVA DTSLHEFLDSFLKFRSRWYDFP+RGANGIVAGVIVGE EL
Sbjct: 121 RELSRLLKLSAREFWTEVARDTSLHEFLDSFLKFRSRWYDFPHRGANGIVAGVIVGECEL 180

Query: 181 SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLT 240
           SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIY HENEDLT
Sbjct: 181 SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYYHENEDLT 240

Query: 241 RILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGENGYSKLQAD 300
           R LVENAIKSQPSIHE LPSVISHFL+IVSMMHQRCNSSLETLFSS S+GE+GYSKLQAD
Sbjct: 241 RSLVENAIKSQPSIHETLPSVISHFLSIVSMMHQRCNSSLETLFSSGSNGESGYSKLQAD 300

Query: 301 FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 360
           FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG
Sbjct: 301 FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 360

Query: 361 FHIVFVSRGDEMMSNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVPVSMKMFPA 420
           F IVFV RGD+MMS+V TSLKMLALR+VSLGWKLLEICYLGDEVFGNDL VPVSMKMFPA
Sbjct: 361 FQIVFVPRGDDMMSSVVTSLKMLALRVVSLGWKLLEICYLGDEVFGNDLAVPVSMKMFPA 420

Query: 421 NVEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSIINRINSLRNNGWILI 480
           NVEDPVIRADI IQTLREINGISQQ PDKQLGQTFLQRM+KNHSI+NRINSLRNNGWI +
Sbjct: 421 NVEDPVIRADIFIQTLREINGISQQAPDKQLGQTFLQRMKKNHSIMNRINSLRNNGWIFV 480

Query: 481 DDEQFDYLSTVVMYTSTSNIKDPSLSKAPAMSHISEVDEDAAMLESKICQIKDLFPEYGS 540
           DDEQFDY+ST+V YT T NIKD SLSKAP MSHISEVDED+AMLESKICQIKDLFPEYGS
Sbjct: 481 DDEQFDYISTIV-YTPTPNIKDSSLSKAPVMSHISEVDEDSAMLESKICQIKDLFPEYGS 540

Query: 541 GFLAACLVAYNQNPEEVIQRILEGTLHSDLQSLDTSLETMPVPNSSATANNRNDKGKGKL 600
           GFLAACLVAYNQNPEEVIQRILEGTLH+DLQSLDTSLETMPVPNSSA   NRNDKGKGKL
Sbjct: 541 GFLAACLVAYNQNPEEVIQRILEGTLHADLQSLDTSLETMPVPNSSAAVINRNDKGKGKL 600

Query: 601 IESPTVAYTHQVSQGKDFPIEGPSVSSTSGGRYVRKSKDDMPYSETLDNRNEADPLRTAA 660
            ES TVAYT QVSQ  D  +EGPSVSST GGRYVRKSKDDMPYSE LDNRNEAD +RTAA
Sbjct: 601 FESSTVAYTDQVSQSNDLMVEGPSVSSTFGGRYVRKSKDDMPYSEALDNRNEADSVRTAA 660

Query: 661 LISQYEYEDEYDDSFDDLGISVAETATEDNEDLVDQKLSSGLGNSSNSTNASSAQNAPSS 720
           L+SQYEYEDEYDDSFDDLGIS+AETATEDNEDLV QKL S LGNSSNS NASSAQNA +S
Sbjct: 661 LVSQYEYEDEYDDSFDDLGISIAETATEDNEDLVGQKLRSDLGNSSNSKNASSAQNASNS 720

Query: 721 KWGSRRKPQYYVKD-----------------ERTNIWTWTRRQ----------LALGAVK 780
           KWGS+RKPQYYVKD                 +  ++ T ++++          L LGAVK
Sbjct: 721 KWGSKRKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQSQKELIHGLGRGGNLPLGAVK 780

Query: 781 KLTESEQDSQPDVSTVDPRENGRKSWGRG-RREGGSGTAVGVPAAPEGQGKQSNVAEVQT 825
           KLTESE+DSQPDVS  DPR+N RKSWGRG RRE GSG+A GV   PEGQGKQ NVAEV  
Sbjct: 781 KLTESEEDSQPDVSAADPRDNVRKSWGRGSRREVGSGSAAGV---PEGQGKQPNVAEVSE 840

BLAST of Lag0037027 vs. ExPASy Swiss-Prot
Match: Q9H1I8 (Activating signal cointegrator 1 complex subunit 2 OS=Homo sapiens OX=9606 GN=ASCC2 PE=1 SV=3)

HSP 1 Score: 110.5 bits (275), Expect = 8.9e-23
Identity = 138/610 (22.62%), Postives = 252/610 (41.31%), Query Frame = 0

Query: 121 ELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAG--VIVGEFE 180
           +L  LL L   +FW +V  D +L + LDS+L++  R +D       G+ +   V+  +  
Sbjct: 65  DLDWLLALPHDKFWCQVIFDETLQKCLDSYLRYVPRKFD------EGVASAPEVVDMQKR 124

Query: 181 LSRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDL 240
           L R VF+   RMS++++        +S    G +L    L D+PK+LD+C ++   N  L
Sbjct: 125 LHRSVFLTFLRMSTHKE---SKDHFISPSAFGEILYNNFLFDIPKILDLCVLFGKGNSPL 184

Query: 241 TRILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGENGYSKLQA 300
            + ++ N    QPS + +L   +   L + S + Q C    +   ++    E       +
Sbjct: 185 LQKMIGNIFTQQPSYYSDLDETLPTILQVFSNILQHCGLQGDGANTTPQKLEERGRLTPS 244

Query: 301 D-----FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLL 360
           D       +++ ++ D   TL +F+  + LA   F           D    LA  ++  +
Sbjct: 245 DMPLLELKDIVLYLCDTCTTLWAFLDIFPLACQTFQ--------KHDFCYRLASFYEAAI 304

Query: 361 PSLQQGFHIVFVSRGDEMMSNVATSLKMLALRIVSLGWKLL-EICYLG------DEVFGN 420
           P ++       +    +++ ++   L     +++ +   +L +IC L       D + G 
Sbjct: 305 PEMESAIKKRRL-EDSKLLGDLWQRLSHSRKKLMEIFHIILNQICLLPILESSCDNIQGF 364

Query: 421 DLPVPVSMKMFPANVEDP--VIRADILIQTLREINGISQ--QVPDKQLGQTFLQRME--- 480
              +   +++F + +++   +   D L     +I+ + Q   V D+      LQ +E   
Sbjct: 365 ---IEEFLQIFSSLLQEKRFLRDYDALFPVAEDISLLQQASSVLDETRTAYILQAVESAW 424

Query: 481 -----------KNHSIINRINSLRNNGWILIDDEQFDYLSTVVMYTSTSNIKDPSLSKAP 540
                      K+ S+I   N   N   + +  E     S+    +        + +  P
Sbjct: 425 EGVDRRKATDAKDPSVIEEPNGEPNG--VTVTAEAVSQASSHPENSEEEECMGAAAAVGP 484

Query: 541 AMSHISEVDEDAAMLESKICQIKDLFPEYGSGFLAACLVAYNQNPEEVIQRILEGTLHSD 600
           AM  +         L+S I Q+KDL P+ G GF+ ACL  Y+ +PE+VI  ILE  L   
Sbjct: 485 AMCGVE--------LDSLISQVKDLLPDLGEGFILACLEYYHYDPEQVINNILEERLAPT 544

Query: 601 LQSLDTSL--ETMPVPNSSATANNRNDKGKGKLIESPTVAYTHQVSQGKDFPIEGPSVSS 660
           L  LD +L  E  P P    T+ +   +     + S       +V +GK    E  + S 
Sbjct: 545 LSQLDRNLDREMKPDPTPLLTSRHNVFQNDEFDVFSRDSVDLSRVHKGKSTRKEENTRSL 604

Query: 661 TSGGRYVRKSKDDMPYSETLDNRNEADPLRTAALISQYEYEDEYDDSFDDLGISVAETAT 697
            +  R V   +        +       P  +    S Y YEDEYDD++D  G  V     
Sbjct: 605 LNDKRAVAAQRQRYEQYSVVVEEVPLQPGESLPYHSVY-YEDEYDDTYD--GNQVGANDA 640

BLAST of Lag0037027 vs. ExPASy Swiss-Prot
Match: Q91WR3 (Activating signal cointegrator 1 complex subunit 2 OS=Mus musculus OX=10090 GN=Ascc2 PE=1 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 9.9e-22
Identity = 146/623 (23.43%), Postives = 256/623 (41.09%), Query Frame = 0

Query: 121 ELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAGVIVGEFELS 180
           +L  LL L   +FW +V  D +L + LDS+L +  R +D  +      VA +   +  L 
Sbjct: 65  DLDWLLALPHDKFWCQVIFDETLQKCLDSYLHYVPRKFD-EWVAPTPEVADM---QNHLH 124

Query: 181 RRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLTR 240
           R VF+   RMS++++        +S    G +L    L D+PK+LD+C ++   N  L +
Sbjct: 125 RSVFLTFLRMSTHKE---SKDHFISPSAFGEILYNNFLFDIPKILDLCVLFGKGNSPLLQ 184

Query: 241 ILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLE-TLFSSSSHGENG-YSKLQA 300
            ++ N    QPS + +L   I   L + S + Q C    + T  +    GE    +    
Sbjct: 185 KMIGNIFTQQPSYYTDLDETIPTILQVFSNILQHCGLQGDGTSTTPQKLGERSPLTPSDM 244

Query: 301 DFLEVID---FINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPS 360
             LE+ D   ++ D   TL +F+  + LA   F           D    LA  +++ +P 
Sbjct: 245 PLLELKDIVLYLCDTSTTLWAFLDIFPLACQTFQ--------KHDFCYRLASFYEMAIPE 304

Query: 361 LQQGFHIVFVSRGDEMMSNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVPV--- 420
           L+            +     +  L  +  R+     KL+E+ ++   +      +P+   
Sbjct: 305 LESAI--------KKRRLEDSKLLGDMWQRLSHSKKKLMEVFHI---ILNQICLLPILES 364

Query: 421 SMKMFPANVEDPVIRADILIQT---LREINGISQQVPDKQLGQTFLQRMEKNHS--IINR 480
           S       +E+ +     L+Q    LR+ +  S    D  L Q     +++  +  I+  
Sbjct: 365 SCDNIQGFIEEFLQIFSSLLQEKRFLRDYDTFSPVAEDISLLQQASSALDETRTAYILQA 424

Query: 481 INSLRNNGWILIDDEQFDYLSTVVMYTSTSN---IKDPSLSKAPAMSHISEVDED----- 540
           + S     W  +D ++   +        ++N   +    +S+ P+     E DE+     
Sbjct: 425 VES----AWEGVDRQKIKDIKDPPRAKGSNNEVTVTAEPVSEMPSQLENLEEDEECMGAA 484

Query: 541 AAM--------LESKICQIKDLFPEYGSGFLAACLVAYNQNPEEVIQRILEGTLHSDLQS 600
           AA+        L+S I Q+KDL P+ G GF+ ACL  Y+ + E+VI  ILE  L  +L  
Sbjct: 485 AALGPAVSGVELDSLISQVKDLLPDLGEGFILACLEHYSYDSEQVINNILEDRLAPELSQ 544

Query: 601 LDTSLETMPVPNSSATANNRNDKGKGKLIESPTVAYTHQVSQGKDFPI---EGPSVSSTS 660
           LD  LE    P+                  +P ++  H + Q  +F +   +   +S   
Sbjct: 545 LDRGLERQVKPD-----------------PTPLLSSRHNIFQNDEFDVFSRDSVDLSRVH 604

Query: 661 GGR----YVRKSKDD-----------MPYSETLDNRNEADPLRTAALISQYEYEDEYDDS 697
            GR     VR   +D             YS  +    E  PL+     +  +YEDEYDD+
Sbjct: 605 KGRRKEENVRSLVNDKQAVVAQWQRYQKYSVVV----EEVPLQPGEYQAD-DYEDEYDDT 633

BLAST of Lag0037027 vs. ExPASy Swiss-Prot
Match: Q54VC4 (Activating signal cointegrator 1 complex subunit 2 homolog OS=Dictyostelium discoideum OX=44689 GN=ascc2 PE=3 SV=2)

HSP 1 Score: 90.1 bits (222), Expect = 1.2e-16
Identity = 151/714 (21.15%), Postives = 287/714 (40.20%), Query Frame = 0

Query: 84  SFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLNRELSRLLKLSAKEFWTEVAGDTSL 143
           SF+ +LP D         E+G+     S+  +  +N +LS LLK     FW+    + SL
Sbjct: 102 SFITFLPAD-------NNEQGS-----SEESILFMNSDLSSLLKFQYNIFWSHCIFNKSL 161

Query: 144 HEFLDSFLKFRSR------------WYDFPYRGANGIVAGVIVGEFE----LSRRVFMVL 203
           +EF+DSFLKF  R              +      N I+        E    L +RVF+VL
Sbjct: 162 NEFIDSFLKFFKRDNQPIISLVNNNSVNNNNNNNNSIIKKGSKDFNESKKLLFKRVFLVL 221

Query: 204 YRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLTRILVENAI 263
            RMS  ++     +  ++ + +  L+   KL  +PKL DI ++Y+    D    ++++  
Sbjct: 222 VRMSLQQE----KSGFITREFYSDLIYKNKLFTIPKLFDIVSLYSSHARDAVTTMIQSIF 281

Query: 264 KSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGENGYSKLQADFLEVI--- 323
            +QP+ +++L   + HF  I            +TLF      E   S L  + L+++   
Sbjct: 282 DTQPNYYKDL---LQHFQLI-----------SKTLF------ELNQSLLNRNLLDLLRLE 341

Query: 324 -DFINDAIVTLDSFVAAYRLAA--IFFSSAVEISCGNED----LLGTLARLHDLLLPSLQ 383
            +++ D +  L+ F+  + + +  +F    + +  G  D    +LG L   ++ ++P   
Sbjct: 342 DNYLMDIVYNLEQFIRIFPMGSHQLFDEYLMNLEKGGGDGNGGVLGWLTYFYEYIIPIFN 401

Query: 384 QGFH-----------IVFVSRGDEMMSNVATSLK-MLALRIVSLGWKLLEICYLGDEVFG 443
           +               ++V     ++S   T  +    +++  L   + + C L  +   
Sbjct: 402 KENQKASNKQVNLTPSIYVPLKQHILSIFHTIFRHHFMIKLEQLQLLIQDPCQLCKK--- 461

Query: 444 NDLPV-PVSMKMFPANVEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSI 503
             LP+  +S + F            I+  T    + +  +  D     + L   E+ + +
Sbjct: 462 --LPLDEISHRFFSLLGNITSFSQQIIGSTKSNRSKLFNEYFDNITSASILYDYEQTYKL 521

Query: 504 INRINSLRNNGWILIDDEQFDYL----------------------STVVMYTSTSNIKDP 563
            N ++ L       ID   + Y                       ST ++ ++ S+    
Sbjct: 522 SNFLDKLVTLD-SSIDFTSYTYFMQLIGKPVSQDQKQIQKPKFKKSTTIIDSNISSSSSS 581

Query: 564 SLSKAP--AMSHISEV--DEDAAMLESKICQIKDLFPEYGSGFLAACLVAYNQNPEEVIQ 623
           S S +P  A + +  +  +    M   KI Q+K LFP+ G  F+  CL  YNQ+ E+VI 
Sbjct: 582 SSSSSPSNATTTVKPILSNNPVIMNTVKIDQVKSLFPDLGDWFVHCCLKYYNQDVEQVIN 641

Query: 624 RIL-EGTLHSDLQSLDTSL--------ETMPVPNSSATANNRNDKGKGKLIESPTVAYTH 683
            +  + +L   L+S+D SL        + +P P ++ T     DK       + T     
Sbjct: 642 ALCDDSSLPPHLKSMDRSLSSDPSQPSKNIPTPTTTTTTTTTTDKPNNTTTATTT----- 701

Query: 684 QVSQGKDFPIEGPSVSSTSGGRYVRKSKDDMPYSETLDNRNEADPLRTAALISQYEYEDE 723
                        + ++TS  + + KS +++  S                +  +  Y+++
Sbjct: 702 -------------TTTTTSSSKSLEKSMNELNIS----------------IGYEKIYDEK 733

BLAST of Lag0037027 vs. ExPASy TrEMBL
Match: A0A6J1FF14 (activating signal cointegrator 1 complex subunit 2-like OS=Cucurbita moschata OX=3662 GN=LOC111443354 PE=4 SV=1)

HSP 1 Score: 1345.1 bits (3480), Expect = 0.0e+00
Identity = 721/855 (84.33%), Postives = 756/855 (88.42%), Query Frame = 0

Query: 1   MSNRYNQDG-NKGLRKDQKKYVPKNLNQSTRELPNPKPTLSTSLRQSLPKPSDSAAASTT 60
           MSNRYN DG NKGLRK+QKKY+PKN  QST E PNPKP LSTSLRQSLPKPSDSAA S++
Sbjct: 1   MSNRYNHDGNNKGLRKEQKKYIPKNQIQSTNERPNPKPNLSTSLRQSLPKPSDSAAVSSS 60

Query: 61  AAPSMSRIHMGANGDWVSSRGSGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 120
           A PSMSRI MGANGDWVSSR +GGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN
Sbjct: 61  ATPSMSRIQMGANGDWVSSRATGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 120

Query: 121 RELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAGVIVGEFEL 180
           RELSRLLKLSAKEFWTEVA DTSLHEFLDSFLKFRSRWYDFP+RGANGIVAGVIVGE EL
Sbjct: 121 RELSRLLKLSAKEFWTEVARDTSLHEFLDSFLKFRSRWYDFPHRGANGIVAGVIVGECEL 180

Query: 181 SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLT 240
           SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIY HENEDLT
Sbjct: 181 SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYYHENEDLT 240

Query: 241 RILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGENGYSKLQAD 300
           R LVENAIKSQPSIHE LPSVISHFL+IVSMMHQRCNSSLETLFSSSS GE+GYSKLQAD
Sbjct: 241 RTLVENAIKSQPSIHETLPSVISHFLSIVSMMHQRCNSSLETLFSSSSSGESGYSKLQAD 300

Query: 301 FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 360
           FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG
Sbjct: 301 FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 360

Query: 361 FHIVFVSRGDEMMSNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVPVSMKMFPA 420
           F IVFV RGD+MMS+V TSLKMLALR+VSLGWKLLEICYLGDEVFGNDL VPVSMKMFPA
Sbjct: 361 FQIVFVPRGDDMMSSVVTSLKMLALRVVSLGWKLLEICYLGDEVFGNDLAVPVSMKMFPA 420

Query: 421 NVEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSIINRINSLRNNGWILI 480
           NVEDPVIRADI IQTLREINGISQQ PDKQLGQTFLQRMEKNHS++NRINSLRNNGWI +
Sbjct: 421 NVEDPVIRADIFIQTLREINGISQQAPDKQLGQTFLQRMEKNHSVMNRINSLRNNGWIFV 480

Query: 481 DDEQFDYLSTVVMYTSTSNIKDPSLSKAPAMSHISEVDEDAAMLESKICQIKDLFPEYGS 540
           DDEQFDY+S +V YT T NIKD SLSKAP MSHISEVDED+AMLESKICQIKDLFPEYGS
Sbjct: 481 DDEQFDYISKIV-YTPTPNIKDSSLSKAPVMSHISEVDEDSAMLESKICQIKDLFPEYGS 540

Query: 541 GFLAACLVAYNQNPEEVIQRILEGTLHSDLQSLDTSLETMPVPNSSATANNRNDKGKGKL 600
           GFLAACLVAYNQNPEEVIQRILEGTLH+DLQSLDTSLETMPVPNSSA A NRNDKGKGKL
Sbjct: 541 GFLAACLVAYNQNPEEVIQRILEGTLHADLQSLDTSLETMPVPNSSAAAINRNDKGKGKL 600

Query: 601 IESPTVAYTHQVSQGKDFPIEGPSVSSTSGGRYVRKSKDDMPYSETLDNRNEADPLRTAA 660
            ES TVAYT QVSQ KD  +EGPSVSST  GRYVRKSKDDMPYSETLD+RNEAD +RTA+
Sbjct: 601 FESSTVAYTDQVSQSKDLMVEGPSVSSTFSGRYVRKSKDDMPYSETLDSRNEADSVRTAS 660

Query: 661 LISQYEYEDEYDDSFDDLGISVAETATEDNEDLVDQKLSSGLGNSSNSTNASSAQNAPSS 720
           L+SQYEYEDEYDDSFDDLGIS+AETATEDNEDLV QKLSS LGNSSNS NASSAQNA +S
Sbjct: 661 LVSQYEYEDEYDDSFDDLGISIAETATEDNEDLVSQKLSSDLGNSSNSKNASSAQNASNS 720

Query: 721 KWGSRRKPQYYVKD-----------------ERTNIWTWTRRQ----------LALGAVK 780
           KWGS+RKPQYYVKD                 +  ++ T  +++          L LGAVK
Sbjct: 721 KWGSKRKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQAQKELIHGLGRGGNLPLGAVK 780

Query: 781 KLTESEQDSQPDVSTVDPRENGRKSWGRG-RREGGSGTAVGVPAAPEGQGKQSNVAEVQT 827
           KLTESEQDSQPDVS  DPR+N RKSWGRG RRE GSG+A GV   PEGQGKQ NVAEV  
Sbjct: 781 KLTESEQDSQPDVSAADPRDNVRKSWGRGSRREVGSGSAAGV---PEGQGKQPNVAEV-- 840

BLAST of Lag0037027 vs. ExPASy TrEMBL
Match: A0A6J1IKZ3 (activating signal cointegrator 1 complex subunit 2-like OS=Cucurbita maxima OX=3661 GN=LOC111477182 PE=4 SV=1)

HSP 1 Score: 1341.3 bits (3470), Expect = 0.0e+00
Identity = 717/853 (84.06%), Postives = 753/853 (88.28%), Query Frame = 0

Query: 1   MSNRYNQDG-NKGLRKDQKKYVPKNLNQSTRELPNPKPTLSTSLRQSLPKPSDSAAASTT 60
           MSNRYN DG NKG RK+QKKY+PKN  QST E PNPKP LSTSLRQSLPKPSDSAA S++
Sbjct: 1   MSNRYNHDGSNKGRRKEQKKYIPKNQIQSTNERPNPKPNLSTSLRQSLPKPSDSAAVSSS 60

Query: 61  AAPSMSRIHMGANGDWVSSRGSGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 120
           A PSMSRI MGANGDWVSSR +GGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN
Sbjct: 61  ATPSMSRIQMGANGDWVSSRATGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 120

Query: 121 RELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAGVIVGEFEL 180
           RELSRLLKLSA+EFWTEVA DTSLHEFLDSFLKFRSRWYDFP+RGANGIVAGVIVGE EL
Sbjct: 121 RELSRLLKLSAREFWTEVARDTSLHEFLDSFLKFRSRWYDFPHRGANGIVAGVIVGECEL 180

Query: 181 SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLT 240
           SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIY HENEDLT
Sbjct: 181 SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYYHENEDLT 240

Query: 241 RILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGENGYSKLQAD 300
           R LVENAIKSQPSIHE LPSVISHFL+IVSMMHQRCNSSLETLFSS S+GE+GYSKLQAD
Sbjct: 241 RSLVENAIKSQPSIHETLPSVISHFLSIVSMMHQRCNSSLETLFSSGSNGESGYSKLQAD 300

Query: 301 FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 360
           FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG
Sbjct: 301 FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 360

Query: 361 FHIVFVSRGDEMMSNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVPVSMKMFPA 420
           F IVFV RGD+MMS+V TSLKMLALR+VSLGWKLLEICYLGDEVFGNDL VPVSMKMFPA
Sbjct: 361 FQIVFVPRGDDMMSSVVTSLKMLALRVVSLGWKLLEICYLGDEVFGNDLAVPVSMKMFPA 420

Query: 421 NVEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSIINRINSLRNNGWILI 480
           NVEDPVIRADI IQTLREINGISQQ PDKQLGQTFLQRM+KNHSI+NRINSLRNNGWI +
Sbjct: 421 NVEDPVIRADIFIQTLREINGISQQAPDKQLGQTFLQRMKKNHSIMNRINSLRNNGWIFV 480

Query: 481 DDEQFDYLSTVVMYTSTSNIKDPSLSKAPAMSHISEVDEDAAMLESKICQIKDLFPEYGS 540
           DDEQFDY+ST+V YT T NIKD SLSKAP MSHISEVDED+AMLESKICQIKDLFPEYGS
Sbjct: 481 DDEQFDYISTIV-YTPTPNIKDSSLSKAPVMSHISEVDEDSAMLESKICQIKDLFPEYGS 540

Query: 541 GFLAACLVAYNQNPEEVIQRILEGTLHSDLQSLDTSLETMPVPNSSATANNRNDKGKGKL 600
           GFLAACLVAYNQNPEEVIQRILEGTLH+DLQSLDTSLETMPVPNSSA   NRNDKGKGKL
Sbjct: 541 GFLAACLVAYNQNPEEVIQRILEGTLHADLQSLDTSLETMPVPNSSAAVINRNDKGKGKL 600

Query: 601 IESPTVAYTHQVSQGKDFPIEGPSVSSTSGGRYVRKSKDDMPYSETLDNRNEADPLRTAA 660
            ES TVAYT QVSQ  D  +EGPSVSST GGRYVRKSKDDMPYSE LDNRNEAD +RTAA
Sbjct: 601 FESSTVAYTDQVSQSNDLMVEGPSVSSTFGGRYVRKSKDDMPYSEALDNRNEADSVRTAA 660

Query: 661 LISQYEYEDEYDDSFDDLGISVAETATEDNEDLVDQKLSSGLGNSSNSTNASSAQNAPSS 720
           L+SQYEYEDEYDDSFDDLGIS+AETATEDNEDLV QKL S LGNSSNS NASSAQNA +S
Sbjct: 661 LVSQYEYEDEYDDSFDDLGISIAETATEDNEDLVGQKLRSDLGNSSNSKNASSAQNASNS 720

Query: 721 KWGSRRKPQYYVKD-----------------ERTNIWTWTRRQ----------LALGAVK 780
           KWGS+RKPQYYVKD                 +  ++ T ++++          L LGAVK
Sbjct: 721 KWGSKRKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQSQKELIHGLGRGGNLPLGAVK 780

Query: 781 KLTESEQDSQPDVSTVDPRENGRKSWGRG-RREGGSGTAVGVPAAPEGQGKQSNVAEVQT 825
           KLTESE+DSQPDVS  DPR+N RKSWGRG RRE GSG+A GV   PEGQGKQ NVAEV  
Sbjct: 781 KLTESEEDSQPDVSAADPRDNVRKSWGRGSRREVGSGSAAGV---PEGQGKQPNVAEVSE 840

BLAST of Lag0037027 vs. ExPASy TrEMBL
Match: A0A6J1FRD2 (uncharacterized protein LOC111446220 OS=Cucurbita moschata OX=3662 GN=LOC111446220 PE=4 SV=1)

HSP 1 Score: 1314.7 bits (3401), Expect = 0.0e+00
Identity = 700/849 (82.45%), Postives = 746/849 (87.87%), Query Frame = 0

Query: 1    MSNRYNQDGNKGLRKDQKKYVPKNLNQSTRELPNPKPTLSTSLRQSLPKPSDSAAASTTA 60
            MSNRYNQDGNKGLRKDQKKY+PKN NQS+++L NPKPTLS S R SLPK SDSAA ST A
Sbjct: 1331 MSNRYNQDGNKGLRKDQKKYIPKNQNQSSKQLLNPKPTLSASFRHSLPKSSDSAAVSTAA 1390

Query: 61   APSMSRIHMGANGDWVSSRGSGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLNR 120
            APS+SRI MG +GDWVSSR SGGSFVNYLPQDEAVA+GLRAEEGALDP+ESQRVVDL NR
Sbjct: 1391 APSLSRIQMGVSGDWVSSRVSGGSFVNYLPQDEAVASGLRAEEGALDPVESQRVVDLSNR 1450

Query: 121  ELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAGVIVGEFELS 180
            ELSRLLKLSAKEFWTEVA DTSLHEFLDSFLKFRSRWYDFP+RGANGIVAG+I+GEFEL 
Sbjct: 1451 ELSRLLKLSAKEFWTEVARDTSLHEFLDSFLKFRSRWYDFPHRGANGIVAGIIIGEFELC 1510

Query: 181  RRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLTR 240
            RRVFMVLYRMSSNRDPGARAADSLS+KDHGVLLQGKKLLDLPKLLDICAIYNHENEDLTR
Sbjct: 1511 RRVFMVLYRMSSNRDPGARAADSLSIKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLTR 1570

Query: 241  ILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGENGYSKLQADF 300
            ILVENAIKSQ SIHENLPSVISHFL+IVSMMHQRCNSSLETL S+SSH    +SKLQADF
Sbjct: 1571 ILVENAIKSQLSIHENLPSVISHFLSIVSMMHQRCNSSLETLISTSSH---EHSKLQADF 1630

Query: 301  LEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQGF 360
            LEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVE+SCGNEDLLGTLARLHD+LLPSLQQGF
Sbjct: 1631 LEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEVSCGNEDLLGTLARLHDILLPSLQQGF 1690

Query: 361  HIVFVSRGDEMMSNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVPVSMKMFPAN 420
             IVFV + D+M+SNVA SLKMLALRIVSLGWKLLEICYLGDEVFG DLPVPVSMKMFPA+
Sbjct: 1691 QIVFVPQEDDMVSNVAKSLKMLALRIVSLGWKLLEICYLGDEVFGKDLPVPVSMKMFPAS 1750

Query: 421  VEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSIINRINSLRNNGWILID 480
            VEDPVIRADILIQTLREI+GISQQVPDKQLGQTFLQ MEKNHSIINRINSLRNNGWI +D
Sbjct: 1751 VEDPVIRADILIQTLREIHGISQQVPDKQLGQTFLQHMEKNHSIINRINSLRNNGWIYVD 1810

Query: 481  DEQFDYLSTVVMYTSTSNIKDPSLSKAPAMSHISEVDEDAAMLESKICQIKDLFPEYGSG 540
            DEQFDYLST+VM   TSNIKDPSLSK P M HISEVDEDAAMLESKICQIKDLFPEYG+G
Sbjct: 1811 DEQFDYLSTIVMCIPTSNIKDPSLSKTPVMRHISEVDEDAAMLESKICQIKDLFPEYGNG 1870

Query: 541  FLAACLVAYNQNPEEVIQRILEGTLHSDLQSLDTSLETMPVPNSSATANNRNDKGKGKLI 600
            FLAACLVAYNQNPEEVIQRILEGTLH+DLQSLDTSLETMPVPNS  TAN RNDKGKGKLI
Sbjct: 1871 FLAACLVAYNQNPEEVIQRILEGTLHADLQSLDTSLETMPVPNSGTTANGRNDKGKGKLI 1930

Query: 601  ESPTVAYTHQVSQGK-DFPIEGPSVSSTSGGRYVRKSKDDMPYSETLDNRNEADPLRTAA 660
            ES TVAYT QVS  K D PIEGPSVSS S GRYVRKSKD MP S+TLD+RNEADP+RTAA
Sbjct: 1931 ESSTVAYTDQVSGCKEDIPIEGPSVSSASAGRYVRKSKDGMPNSQTLDSRNEADPVRTAA 1990

Query: 661  LISQYEYEDEYDDSFDDLGISVAETATEDNEDLVDQKLSSGLGNSSNSTNASSAQNAPSS 720
            LISQYEYEDEYDDSFDDLGIS+AETATEDN D V +K S+ LGNSSNST A SA++AP+ 
Sbjct: 1991 LISQYEYEDEYDDSFDDLGISIAETATEDNGDFVGRKPSADLGNSSNSTTAGSARSAPNP 2050

Query: 721  KWGSRRKPQYYVKD-----------------ERTNIWTWTRRQ----------LALGAVK 780
            KWGSRR+PQ+YVKD                 E  ++ T  +++          L LGAV+
Sbjct: 2051 KWGSRRRPQFYVKDGKNYSYKVAGSVAVSNSEEASLVTQAQKELIYGLGRGGNLPLGAVR 2110

Query: 781  KLTESEQDSQPDVSTVDPRENGRKSWGRGRREGGSGTAVGVPAAPEGQGKQSNVAEVQTG 822
            KLTESEQDS PDVS VDPR+NGRKSWGRGRREGG+GTA   P+ PE QGKQ NVAEV   
Sbjct: 2111 KLTESEQDSPPDVSAVDPRDNGRKSWGRGRREGGNGTA---PSVPEEQGKQPNVAEVSDR 2170

BLAST of Lag0037027 vs. ExPASy TrEMBL
Match: A0A1S4DZ72 (uncharacterized protein LOC103493893 OS=Cucumis melo OX=3656 GN=LOC103493893 PE=4 SV=1)

HSP 1 Score: 1312.4 bits (3395), Expect = 0.0e+00
Identity = 699/849 (82.33%), Postives = 739/849 (87.04%), Query Frame = 0

Query: 1    MSNRYNQDGNKGLRKDQKKYVPKNLNQSTRELPNPKPTLSTSLRQSLPKPSDSAAASTTA 60
            MSNRYN DGNKGLRKDQKKY+PKN  QST ELPNPKPTLSTSLRQSLP PSDS     TA
Sbjct: 1385 MSNRYNHDGNKGLRKDQKKYIPKNQIQSTHELPNPKPTLSTSLRQSLPNPSDS-----TA 1444

Query: 61   APSMSRIHMGANGDWVSSRGSGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLNR 120
            APS+SRI MGANGDWVSSR SGGSFVNYLPQDEAVATGLRAEEGALDP+ESQRVVDLLNR
Sbjct: 1445 APSVSRIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNR 1504

Query: 121  ELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAGVIVGEFELS 180
            ELSRLLKLSAKEFW EVA DTSLHEFLDSFLKFR+RWYDFP+RGA G VAGVIVGE+ELS
Sbjct: 1505 ELSRLLKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELS 1564

Query: 181  RRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLTR 240
            RRVFM LYRMSSNRDPGARAADSLSLKDHGVLLQ KKLLDLPKLLDICAIY+HENEDLTR
Sbjct: 1565 RRVFMALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTR 1624

Query: 241  ILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGENGYSKLQADF 300
            ILV+NAIKSQPSIHE LPSVISHFL+IVSMMH+RC+SSLETLFSSSSHG +GY KLQADF
Sbjct: 1625 ILVDNAIKSQPSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQADF 1684

Query: 301  LEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQGF 360
            LEVIDFINDAIV LDSFVAAYRLAAIFFSSAVEISCGNEDLLG LARLHDLLLPSLQQGF
Sbjct: 1685 LEVIDFINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPSLQQGF 1744

Query: 361  HIVFVSRGDEMMSNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVPVSMKMFPAN 420
             IV + +GDEM+SNVATSLKMLALRIVS GW LLEICYLGDEVFGNDLPVPVSMKMFPAN
Sbjct: 1745 QIVLMPQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSMKMFPAN 1804

Query: 421  VEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSIINRINSLRNNGWILID 480
            VEDPVIRADILIQTLREINGISQQ   KQLGQTFLQRMEKNHSI+NRINSLRNNGWI +D
Sbjct: 1805 VEDPVIRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFVD 1864

Query: 481  DEQFDYLSTVVMYTSTSNIKDPSLSKAPAMSHISEVDEDAAMLESKICQIKDLFPEYGSG 540
            DEQF+YLST+VMYT TS+ KDPSLSKAP +SH+SEVDEDAAMLESKICQIKDLFPEYGSG
Sbjct: 1865 DEQFNYLSTMVMYTPTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDLFPEYGSG 1924

Query: 541  FLAACLVAYNQNPEEVIQRILEGTLHSDLQSLDTSLETMPVPNSSATANNRNDKGKGKLI 600
            FLAACLVAYNQNPEEVIQRILEGTLH DLQSLDTSLETMPVPNSSATA NR DKGKGKL 
Sbjct: 1925 FLAACLVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLF 1984

Query: 601  ESPTVAYTHQVSQGKDFPIEGPSVSSTSGGRYVRKSKDDMPYSETLDNRNEADPLRTAAL 660
            E  TV YT QVS+GKD P EGPSVSSTS GR+VRKSKDD+PYSETLDNRNEAD +RTAAL
Sbjct: 1985 EPSTVPYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAAL 2044

Query: 661  ISQYEYEDEYDDSFDDLGISVAETATEDNEDLVDQKLSSGLGNSSNSTNASSAQNAPSSK 720
            ISQYEYEDEYDDSFDDLGIS+AETATEDNEDLV QK SS LG+SSNSTN SSAQNAP+SK
Sbjct: 2045 ISQYEYEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNSK 2104

Query: 721  WGSRRKPQYYVKD-----------------ERTNIWTWTRRQ----------LALGAVKK 780
            WGSRR PQYYVKD                 +  ++ T  +++          L LGAVKK
Sbjct: 2105 WGSRRTPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKK 2164

Query: 781  LTESEQDSQPDVSTVDPRENGRKSWGRGRREGGSGTAVG-VPAAPEGQGKQSNVAEVQTG 822
            LTES+QDSQP  + VDPR+N RK+WGRGRRE       G  P  PEG+GKQ NVAE    
Sbjct: 2165 LTESQQDSQPASAVVDPRDNVRKTWGRGRRERERERESGAAPGMPEGEGKQPNVAEASDR 2224

BLAST of Lag0037027 vs. ExPASy TrEMBL
Match: A0A6J1CEQ5 (activating signal cointegrator 1 complex subunit 2 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011005 PE=4 SV=1)

HSP 1 Score: 1306.6 bits (3380), Expect = 0.0e+00
Identity = 686/853 (80.42%), Postives = 745/853 (87.34%), Query Frame = 0

Query: 1   MSNRYNQDGNKGLRKDQKKYVPKNLNQSTRELPNPKPTLSTSLRQSLPKPSDSAAASTTA 60
           MSNRYNQD NKGLRKDQ+KY+PKN N ++++LPNPKPTLSTSLRQSLPKPSDSAA +T  
Sbjct: 1   MSNRYNQDANKGLRKDQRKYIPKNQNHASKQLPNPKPTLSTSLRQSLPKPSDSAAVTTAT 60

Query: 61  APSMSRIHMGANGDWVSSRGSGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLNR 120
           APSMSRI MGANGDWVSS  SGGSFVNYLPQDEAVATGLRAE+GALDP+ESQRVVDLLNR
Sbjct: 61  APSMSRIQMGANGDWVSSGASGGSFVNYLPQDEAVATGLRAEDGALDPVESQRVVDLLNR 120

Query: 121 ELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAGVIVGEFELS 180
           ELSRLLKLSAKEFWTEVA DTSLHEFLDSFLKFRSRWYDFP+RGANG+VAGVIVGE ELS
Sbjct: 121 ELSRLLKLSAKEFWTEVAKDTSLHEFLDSFLKFRSRWYDFPHRGANGLVAGVIVGELELS 180

Query: 181 RRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLTR 240
           RRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIY+HENEDLTR
Sbjct: 181 RRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYSHENEDLTR 240

Query: 241 ILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGENGYSKLQADF 300
           ILVENA+  QPSIHENL SV SHFL IVSMMHQRC+SSLETLFSS+SHGE+GYS+LQADF
Sbjct: 241 ILVENAMNLQPSIHENLSSVASHFLGIVSMMHQRCSSSLETLFSSNSHGESGYSRLQADF 300

Query: 301 LEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQGF 360
           LEVIDFINDAIVTLDS+V AYR AAIFFSSAVE+SCGNEDLLGTLARLHDLLLPSLQQGF
Sbjct: 301 LEVIDFINDAIVTLDSYVTAYRSAAIFFSSAVEVSCGNEDLLGTLARLHDLLLPSLQQGF 360

Query: 361 HIVFVSRGDEMMSNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVPVSMKMFPAN 420
            IVFV RGD+M+SNVATSLKMLA+RIV  GWKLLE CYLGD+VF NDLPVPVSMKMFPAN
Sbjct: 361 QIVFVPRGDDMISNVATSLKMLAIRIVRFGWKLLEACYLGDDVFRNDLPVPVSMKMFPAN 420

Query: 421 VEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSIINRINSLRNNGWILID 480
           VEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNH I+NRINSLRNNGW+L+D
Sbjct: 421 VEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHFIMNRINSLRNNGWVLVD 480

Query: 481 DEQFDYLSTVVMYTSTSNIKDPSLSKAPAMSHISEVDEDAAMLESKICQIKDLFPEYGSG 540
           DEQ DYLST++MYT+TSN K+P     P M+H+SEVDEDAAMLESKICQIKDLFPEYGSG
Sbjct: 481 DEQLDYLSTILMYTTTSNTKEP-----PVMNHVSEVDEDAAMLESKICQIKDLFPEYGSG 540

Query: 541 FLAACLVAYNQNPEEVIQRILEGTLHSDLQSLDTSLETMPVPNSSATANNRNDKGKGKLI 600
           FLAACLVAYNQNPEEVIQRILEGTLH+DLQSLDTSLETMPVPNS A+ +NRNDKGKGKLI
Sbjct: 541 FLAACLVAYNQNPEEVIQRILEGTLHADLQSLDTSLETMPVPNSGASVDNRNDKGKGKLI 600

Query: 601 ESPTVAYTHQVSQGKDFPIEGPSVSSTSGGRYVRKSKDDMPYSETLDNRNEADPLRTAAL 660
           ES  V YT+QV +GKD P+EGPSVSS SGGR+VRKSKD+ PYSETLD RNEAD +RTAAL
Sbjct: 601 ESSAVPYTNQVPRGKDLPMEGPSVSSASGGRFVRKSKDESPYSETLDRRNEADTVRTAAL 660

Query: 661 ISQYEYEDEYDDSFDDLGISVAETATEDNEDLVDQKLSSGLGNSSNSTNASSAQNAPSSK 720
           ISQYEYEDEYDDSFDDLGIS+AETATEDNEDLV QKLSS LGNSSNS   SSAQ+A +SK
Sbjct: 661 ISQYEYEDEYDDSFDDLGISIAETATEDNEDLVGQKLSSDLGNSSNSMRGSSAQSASNSK 720

Query: 721 WGSRRKPQYYVKD-----------------ERTNIWTWTRRQ----------LALGAVKK 780
           WGSR KPQ+YVKD                 +  ++    +++          L LGAV+K
Sbjct: 721 WGSRSKPQFYVKDGKNYSYKVAGSIAVSNSDEASLVNQAQKELIYGLGRGGNLPLGAVRK 780

Query: 781 LTESEQDSQPDVSTVDPRENGRKSWGRGRREGGSGTAVGVPAAPEGQGKQSNVAEVQTGG 827
           LTESEQDS+P VS VDPR+  RKSWGRGRREGG+  A+      EGQGKQSNVAEV   G
Sbjct: 781 LTESEQDSEPVVSAVDPRDGVRKSWGRGRREGGTDAAI-----TEGQGKQSNVAEVSDRG 840

BLAST of Lag0037027 vs. TAIR 10
Match: AT1G27752.1 (Ubiquitin system component Cue protein )

HSP 1 Score: 747.7 bits (1929), Expect = 1.0e-215
Identity = 444/876 (50.68%), Postives = 578/876 (65.98%), Query Frame = 0

Query: 1   MSNRYNQDGNKGLR-----KDQKKYVPKNLNQSTRELPNPKP-TLSTSLRQSLPKPSDSA 60
           MSNR +   ++  R       Q+K+VPK +N +      P P +LS+SLRQS      S 
Sbjct: 1   MSNRRSNRQDENTRYVPKGHQQQKFVPKPMNPTPTSNSTPFPVSLSSSLRQS----DSSG 60

Query: 61  AASTTAAPSMSRIHMGANGDWVSSRG---SGGSFVNYLPQDEAVATGLRAEEGALDPMES 120
           A+S  +A   SR+ +G  G  VSS+     GGSFVNYLPQDEAVA GL  ++G LDP+ES
Sbjct: 61  ASSRVSASGGSRVRIGDQGQLVSSKSPAQGGGSFVNYLPQDEAVAAGLGPDDGGLDPVES 120

Query: 121 QRVVDLLNRELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAG 180
           Q VVDLLNREL+RLLKL+ ++FW EVA D SLH+FLDSFL+FRSRWYDFP+ G  GIVAG
Sbjct: 121 QGVVDLLNRELTRLLKLNPRDFWREVASDASLHDFLDSFLQFRSRWYDFPFHGVKGIVAG 180

Query: 181 VIVGEFELSRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIY 240
           VIVGE EL RRVFMVLYR+SSNRDPGA+AADSLS KDH VLLQ KKLLDLPKLLDICAIY
Sbjct: 181 VIVGELELCRRVFMVLYRISSNRDPGAKAADSLSQKDHEVLLQDKKLLDLPKLLDICAIY 240

Query: 241 NHENEDLTRILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGEN 300
            HEN +LT+ L+ENA+KSQ  I E+L  ++SHFL I+  MH RC SSLETL SS++  ++
Sbjct: 241 GHENAELTKSLIENAVKSQNGISESLNMMLSHFLGILHTMHHRCTSSLETLVSSANSEDH 300

Query: 301 GYSKLQADFLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDL 360
           G  +L +D LEV+DFIND +V+LD+F++AY  A    +  VE S G+++LL +L RLHD 
Sbjct: 301 GRRQLHSDLLEVMDFINDGVVSLDAFISAYTPAVFILACPVETSYGSDELLRSLVRLHDS 360

Query: 361 LLPSLQQGFHIVFVSRGDEMMSNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVP 420
           LLPSL +GF ++F     + +S+++TSL ML+ RI SL WK+L+ICYL ++ F ++  +P
Sbjct: 361 LLPSLHRGFQVLFKDEDHDSLSDISTSLNMLSTRIGSLCWKILDICYLSNDKFNHETSIP 420

Query: 421 VSMKMFPANVEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSIINRINSL 480
              KMFP+ VEDP++RADILIQT REI+G+S+Q  + +     LQ++EKN+ II+R+ SL
Sbjct: 421 AVTKMFPSRVEDPMVRADILIQTFREISGLSEQSLESK--NRLLQKIEKNYRIIDRLRSL 480

Query: 481 RNNGWILIDDEQFDYLSTVVMYTS-TSNIKDPSLSKAPAMSHISEVDEDAAMLESKICQI 540
           +N GWI ++DEQ  YLS ++++++ T ++K+  L      +    +DE+A +++SKI QI
Sbjct: 481 QNAGWISMEDEQLQYLSMIMLHSADTFSMKESPLLLTDGRNAEELMDENAVVMQSKISQI 540

Query: 541 KDLFPEYGSGFLAACLVAYNQNPEEVIQRILEGTLHSDLQSLDTSLETMPVPNSSATANN 600
           KD+FPEYG+GFLAACL AYNQNPEEVIQRILEGTLH DLQ LDTSLETMP P S+ T  +
Sbjct: 541 KDIFPEYGNGFLAACLEAYNQNPEEVIQRILEGTLHEDLQRLDTSLETMPQPKSAPTLRS 600

Query: 601 RNDKGKGKLIESPTVAYTHQVSQGKDFPIEGPSV-----SSTSGGRYVRKSKDDMPYSET 660
           + DKGKGKLIES T +    +   K  PI  PS+     SS + GR+VRK KDD P  + 
Sbjct: 601 K-DKGKGKLIESDTSSSASAIYTEK--PITRPSLPASSASSATVGRFVRKPKDDTPSYKI 660

Query: 661 LDNRNEADPLRTAALISQYEYEDEYDDSFDDLGISVAETATEDNEDLVDQKLSSGLGNSS 720
           LD R E+D  R AAL++QYEY+DEYDDSFDDLG+S+AE+ TE+         S   GN +
Sbjct: 661 LDARKESDRERNAALLAQYEYDDEYDDSFDDLGLSIAESGTEE---------SGAFGNRA 720

Query: 721 NSTNASSAQNAPSSKWGSRRKPQYYVKDERTNIWTWTRR--------------------- 780
            S      + + + KWGSR+ PQ+YVKD +   +                          
Sbjct: 721 GS------EPSDAPKWGSRKNPQFYVKDGKNYSYKVAGAVAVANANEASLVNEAEGDKIL 780

Query: 781 ------QLALGAVKKLTE------SEQDSQPDVSTVDPRENGRKSWG-RGRREGGSGTAV 827
                  + LGAV+KLTE       +  S  +V+  D RENGR   G RGR  G      
Sbjct: 781 GLGRGGNIPLGAVRKLTEYQAQRDEKGQSNVNVNPSDGRENGRNWRGVRGRGRGVVNREQ 840

BLAST of Lag0037027 vs. TAIR 10
Match: AT1G27752.2 (Ubiquitin system component Cue protein )

HSP 1 Score: 513.8 bits (1322), Expect = 2.5e-145
Identity = 308/655 (47.02%), Postives = 418/655 (63.82%), Query Frame = 0

Query: 213 LQGKKLLDLPKLLDICAIYNHENEDLTRILVENAIKSQPSIHENLPSVISHFLTIVSMMH 272
           ++ KKLLDLPKLLDICAIY HEN +LT+ L+ENA+KSQ  I E+L  ++SHFL I+  MH
Sbjct: 1   MKDKKLLDLPKLLDICAIYGHENAELTKSLIENAVKSQNGISESLNMMLSHFLGILHTMH 60

Query: 273 QRCNSSLETLFSSSSHGENGYSKLQADFLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAV 332
            RC SSLETL SS++  ++G  +L +D LEV+DFIND +V+LD+F++AY  A    +  V
Sbjct: 61  HRCTSSLETLVSSANSEDHGRRQLHSDLLEVMDFINDGVVSLDAFISAYTPAVFILACPV 120

Query: 333 EISCGNEDLLGTLARLHDLLLPSLQQGFHIVFVSRGDEMMSNVATSLKMLALRIVSLGWK 392
           E S G+++LL +L RLHD LLPSL +GF ++F     + +S+++TSL ML+ RI SL WK
Sbjct: 121 ETSYGSDELLRSLVRLHDSLLPSLHRGFQVLFKDEDHDSLSDISTSLNMLSTRIGSLCWK 180

Query: 393 LLEICYLGDEVFGNDLPVPVSMKMFPANVEDPVIRADILIQTLREINGISQQVPDKQLGQ 452
           +L+ICYL ++ F ++  +P   KMFP+ VEDP++RADILIQT REI+G+S+Q  + +   
Sbjct: 181 ILDICYLSNDKFNHETSIPAVTKMFPSRVEDPMVRADILIQTFREISGLSEQSLESK--N 240

Query: 453 TFLQRMEKNHSIINRINSLRNNGWILIDDEQFDYLSTVVMYTS-TSNIKDPSLSKAPAMS 512
             LQ++EKN+ II+R+ SL+N GWI ++DEQ  YLS ++++++ T ++K+  L      +
Sbjct: 241 RLLQKIEKNYRIIDRLRSLQNAGWISMEDEQLQYLSMIMLHSADTFSMKESPLLLTDGRN 300

Query: 513 HISEVDEDAAMLESKICQIKDLFPEYGSGFLAACLVAYNQNPEEVIQRILEGTLHSDLQS 572
               +DE+A +++SKI QIKD+FPEYG+GFLAACL AYNQNPEEVIQRILEGTLH DLQ 
Sbjct: 301 AEELMDENAVVMQSKISQIKDIFPEYGNGFLAACLEAYNQNPEEVIQRILEGTLHEDLQR 360

Query: 573 LDTSLETMPVPNSSATANNRNDKGKGKLIESPTVAYTHQVSQGKDFPIEGPSV-----SS 632
           LDTSLETMP P S+ T  ++ DKGKGKLIES T +    +   K  PI  PS+     SS
Sbjct: 361 LDTSLETMPQPKSAPTLRSK-DKGKGKLIESDTSSSASAIYTEK--PITRPSLPASSASS 420

Query: 633 TSGGRYVRKSKDDMPYSETLDNRNEADPLRTAALISQYEYEDEYDDSFDDLGISVAETAT 692
            + GR+VRK KDD P  + LD R E+D  R AAL++QYEY+DEYDDSFDDLG+S+AE+ T
Sbjct: 421 ATVGRFVRKPKDDTPSYKILDARKESDRERNAALLAQYEYDDEYDDSFDDLGLSIAESGT 480

Query: 693 EDNEDLVDQKLSSGLGNSSNSTNASSAQNAPSSKWGSRRKPQYYVKDERTNIWTWTRR-- 752
           E+         S   GN + S      + + + KWGSR+ PQ+YVKD +   +       
Sbjct: 481 EE---------SGAFGNRAGS------EPSDAPKWGSRKNPQFYVKDGKNYSYKVAGAVA 540

Query: 753 -------------------------QLALGAVKKLTE------SEQDSQPDVSTVDPREN 812
                                     + LGAV+KLTE       +  S  +V+  D REN
Sbjct: 541 VANANEASLVNEAEGDKILGLGRGGNIPLGAVRKLTEYQAQRDEKGQSNVNVNPSDGREN 600

Query: 813 GRKSWG-RGRREGGSGTAVGVPAAPEGQGKQSNV-AEVQTGGKRRQQRPRKEGRG 827
           GR   G RGR  G          + E     S V  E + GG R + R R+ G G
Sbjct: 601 GRNWRGVRGRGRGVVNREQPQEKSNESSNNNSEVNTEAENGGGRGRGRGRRGGGG 635