Lag0036992 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0036992
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	protein DCL homolog, chloroplastic-like
Location	chr2: 2668778 .. 2672677 (+)
RNA-Seq Expression	Lag0036992
Synteny	Lag0036992

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTACTTCTTTGCTAATCAGGGGGCATCCTCTCCTCCGGCTTGCGCTCAGGCAGTGCGGGCTATGTACAGGGATCGTGCAGGTGGCTCGCCGGTCTTGCTGCACTGCCACGGCGGCGTCTACTCCACCGGACGGCAACTTAACATCTGCTGAAAATACTACCTCAGTGTTGAGTGCCAGTGACCCACCCAAGTACCAAAGGTGGGACGAGCCTGATTATCGAAAGTGGAAGAACCAGGAAAAGGAAATTCTCAGCGACATCGAGCCTATCATATCCCTCACAAAAGAGATCCTCCACTCTAATAGGTTTGTCCGGTTTCCGCAATTTCGCCCCGAAGGTTTATTCTCTATGTTACAGGCACTATGTCTTTTCGTTTTAGTTGCTGAGAAACTGTTTGAGAAAAGAATGTTGGAAAATTGTGTTTTTCTTTATGGGCTGCTAGAAAAGAAACCTCTTTAGTTTCGAACAATATATTATATCAAAGCGTAGATTGGAAAAGCAATTTTTGTAAAATTCAAAGACAGACATATAGAACATGTTTTGAGTCAGAACGTAGTAAGAAATTTTCATTTTTAAATAAACCATAAATTTTCCGTCCAAGAACCACCTTTTTACTCACGATAAACTCATGAGCATGAAAGATTGTAAAGCGCAAATGTACCTGAATCTATCTTTAGAAGTTGATTAGCGGGTAGATGGGAATGGTAAAGTAAGGAGAAAAGTTAATATGAAGCGAATAACTTCTTAGTAATTGACCTCAAGTTATTAGGTTTTCTTTACTTCTAATTTGACTCATTCTGAAATGGTATCTAGTATGTTGTTTCATGGTAATATGATAACTACATTCGTAAGTCAACTCCCATCTAGATCATTTTATTTGAATCACTTGATAAATAGCATTTGACATTTACCTGCGTGATCCCGGATGTTGATACCTTCCAACAACAATAGCAACTACGGATAAGCTTTCAAATATATTATAGTAGAAAGAAAAATGAATACTGGAATTAGGTTTAATGTCAGTGTGCTTTATGAATCATTTCTTAATTTTCTTATGGATTTGGGATCAACTGTCTATGGCCTCTTCAAAGAACGAGTGTGCCTTTGACTTTACTTTATACCATCAGAGGGACATACTTCTCCATTATGTGTCGTTTGTCAGCTGTGTGCTCGTAGCTGAAATTTTGCTGTTATTTTCATTCAGTCAATGAATTTAACAATTAGAACCCAGTTAGAAAGAAAAAACTTGCAATATCAAAACTGTGGTTGTGACCTTATTAAAGTATTGGTCTAATATATAGAACTCTCTCGAACATGAATTGTCTTCTTAAATAATGAGCTCATCTTTCATCTTTATGCGAGTAACCAGATGCACAGCTGGGGGGAGGGAATATTATTGCGTGTTTGGTAGTTTCATTTTGGGAGAGATTCAAGCCTCTCAAATTGTCTAAGACTCGCTTCTTTTTCCTTTTATTTTGTGCTCTTTATTATTAGCAAACTCAGATTTCCGTTGAAAATATTTACATTATTTCTTGACACACAATATTTGTTACGTTCTCTTCCACTTGGTTGCTTACTGGAGTCATGATTGTAATCTTTATGCTCCTCTTTGATCTTTTCCATTTTGAATAGTATTCAATGCATATTATTATGAAAATTAAGACCAAAAGTTATGCTCATTGAGAACTAGTGGATGTCCATGAAGCTTTTAACTGGTAAAAGCAGTCAGCGGTCATTTCCTCAATGTAGGGTAGGTTGCCAGGTTGGCATGTTCAGGAGCTGGTTTCATTAGGCATGTTTCCATATAATATATTGCCCATGATTCTGTTTTACAAACAAACTTTGCTCCTACAATAAGTTCCCTTCTCTTAAGGACTTCTATTTTTTATACTAGGTATGTAGATGGGGAGCGGTTGACATCTGAGGTCGAGAGAATTGTGGTTGACAGGCTTCTGGCTCATCATCCACATGCTGAAGATAAAATTGGATGTGGACTTGAATCCATTATGGTATGCTTTGGTCGGCACTAAATGGTTTAGAGTTCTTAAATTGTTTCAAATATGCAGTTTGCATGCTTTTTTTGTCAGGTCGTATAGAAGCAAAAGAAAAGAACACAGATGACATTAGGTATATGGATATTTCCAAAAGCTCAAAATACAAAATTTGACCTCTTACCTATACTACATAGCCCGTACAAGAATTAATCTCTAATAAATTCACCCATTAGGCCATATGTGATAGGACCTCAGAACTAACAAGACATTCTCAAGTGCAGTTGCATGGACTTCATGCTTGTGAGCTCCAGGAACCTTGTGAAATGAATTGGACAACTCTAGCCCTTAAAAGAGATTGGGATGATGGGAACCATGCTGATATAGCCTTGAGATATGAAAGGGTTACAAAGTTGGGCAAGAAAATTGATCTCTTTTTTTTTATAAGAAACATCAAAGGGTATATATATATATATAGAATACCACAAAAAGGGAACAACCTAAGCGCCGGGTAGAGAAAACCCCTCCCAAACACTAATTCATGAGAGCATGTCAGTCGCTAATAATCATAAGCAAACTGTAATTACAAAAGAAATGCTGTATTTTTTACAAGGCTACAAAAATTATCAATAGAAGTCAATTGATCTCTCCCTCAACTCTCTCAATTTGGGCAAGAAATTTGAGATCCCTCCCCTAACTCTTTCAATTCAAAGCTACGTTGAATGTGGCGTGAAAGTCAATAACTTCTGGAATAAAATGTTAAAAGGCTGTCCTACCAATTGTTAGCTTAACGTAGCGTAAAGATAAAAGGTTGGAATTGTACAATAAGTGCAGGAGACACCATGTAAAATGACGAAGATAAATGGTAGAGATTGGTAGAAGTTTCTGGCTCCACATCTCATCTTTACCAAAATATTTGCCCACCCCTATCCTCCAACTCCCTTAATAACGGCCTGTCTTACTAACTTCCTAGCTAATGGCTTAATTGTACCCTAATAACATGTGGTCAAGTAATGGCCTCCAATTGACACCCAACTCTTTGTCAATAAGGGTCACATTTCAACTGCACTTGAAGTCAGCAAGCAATTTCGTATTAAGAGCAGCTCATACTATAATAATACTGAAGATTCTCTGATTAAGACTTTTGATATATGTTATATGATGCTTGCTTATACATTGAAGATCTTTGTGGGCTATCATGCTCTTCCGCTTCTGTTTGTACTGAAGAGATATAGCACGGTTATAAAATGGGACTTATCTGCTGGTGTTGCGAAAGTAGCTTACTGGACTCTGTGGCTGTGTTTTTGTGTTGCTCAATTTTAATCTTGGACCACATTTGAAATAGGTTGCTACATGAGGACGGCACCTTGCTAAGATAGAGTAACTCTGTGGCTCATGGCCTTGCTTTGACAGCCCTCTCCTACTTTGGCTCTTTTTGTTGGTTGTCTTCTTTCCTTTGGATTCAGGATATTTTTATGTCTGTTGGCCAGGCTCCCCGGTTTAATATATATTCCCTTTCAGTAAAAAAAAAAAAGTTGAAAATTGCAAGTGACCTAATTTTCTGTTTTCTATGACCATTGTCTAGAAATCATCATGTTCTTTTTTCCCTGTCCCGCCCACTTCTCATTTTAAGATGTTGTGCACATCACTTTTCATATCAACGAAGCTTACTCATGTCTATTAGAGTTATTTGTTTTTTATTTTAAAAAATTATTTAAGCTATTTTCTGCTGTTGAAGGTTGATCGGCACCCCCAATTTCGGCACTCAAGATGCCTCTTTGTTATAAGGACTGATGGTGGATGGATTGACTTCTCCTATCAAAAATGTCTTCGGGGATATATCCGAAATAAGTACCCATCTTATGCAGAGCGGTTTATTCGAAAACATTTCAAACGCAGTAGTTGA

mRNA sequence

ATGGCTACTTCTTTGCTAATCAGGGGGCATCCTCTCCTCCGGCTTGCGCTCAGGCAGTGCGGGCTATGTACAGGGATCGTGCAGGTGGCTCGCCGGTCTTGCTGCACTGCCACGGCGGCGTCTACTCCACCGGACGGCAACTTAACATCTGCTGAAAATACTACCTCAGTGTTGAGTGCCAGTGACCCACCCAAGTACCAAAGGTGGGACGAGCCTGATTATCGAAAGTGGAAGAACCAGGAAAAGGAAATTCTCAGCGACATCGAGCCTATCATATCCCTCACAAAAGAGATCCTCCACTCTAATAGGTATGTAGATGGGGAGCGGTTGACATCTGAGGTCGAGAGAATTGTGGTTGACAGGCTTCTGGCTCATCATCCACATGCTGAAGATAAAATTGGATGTGGACTTGAATCCATTATGGTTGATCGGCACCCCCAATTTCGGCACTCAAGATGCCTCTTTGTTATAAGGACTGATGGTGGATGGATTGACTTCTCCTATCAAAAATGTCTTCGGGGATATATCCGAAATAAGTACCCATCTTATGCAGAGCGGTTTATTCGAAAACATTTCAAACGCAGTAGTTGA

Coding sequence (CDS)

ATGGCTACTTCTTTGCTAATCAGGGGGCATCCTCTCCTCCGGCTTGCGCTCAGGCAGTGCGGGCTATGTACAGGGATCGTGCAGGTGGCTCGCCGGTCTTGCTGCACTGCCACGGCGGCGTCTACTCCACCGGACGGCAACTTAACATCTGCTGAAAATACTACCTCAGTGTTGAGTGCCAGTGACCCACCCAAGTACCAAAGGTGGGACGAGCCTGATTATCGAAAGTGGAAGAACCAGGAAAAGGAAATTCTCAGCGACATCGAGCCTATCATATCCCTCACAAAAGAGATCCTCCACTCTAATAGGTATGTAGATGGGGAGCGGTTGACATCTGAGGTCGAGAGAATTGTGGTTGACAGGCTTCTGGCTCATCATCCACATGCTGAAGATAAAATTGGATGTGGACTTGAATCCATTATGGTTGATCGGCACCCCCAATTTCGGCACTCAAGATGCCTCTTTGTTATAAGGACTGATGGTGGATGGATTGACTTCTCCTATCAAAAATGTCTTCGGGGATATATCCGAAATAAGTACCCATCTTATGCAGAGCGGTTTATTCGAAAACATTTCAAACGCAGTAGTTGA

Protein sequence

MATSLLIRGHPLLRLALRQCGLCTGIVQVARRSCCTATAASTPPDGNLTSAENTTSVLSASDPPKYQRWDEPDYRKWKNQEKEILSDIEPIISLTKEILHSNRYVDGERLTSEVERIVVDRLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRGYIRNKYPSYAERFIRKHFKRSS

Homology

[Close]

BLAST of Lag0036992 vs. NCBI nr
Match: XP_022135338.1 (protein DCL, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 364.0 bits (933), Expect = 8.1e-97
Identity = 176/196 (89.80%), Postives = 184/196 (93.88%), Query Frame = 0


Query: 1   MATSLLIRGHPLLRLALRQCGLCTGIVQVARRSCCTATAASTPPDGNLTSAENTTSVLSA 60
           MA SLL+RGHPLLRL LR  GLCTGIVQV RRSCCTATAA TPPDGNLTSAEN TSVLS+
Sbjct: 1   MAASLLLRGHPLLRLGLRHRGLCTGIVQVTRRSCCTATAAPTPPDGNLTSAENITSVLSS 60

Query: 61  SDPPKYQRWDEPDYRKWKNQEKEILSDIEPIISLTKEILHSNRYVDGERLTSEVERIVVD 120
           SDPPKY RWDEPDYRKWK+QE+E+L+DIEPIISLTKEILHSNRYVDGERLTS  ERIVV+
Sbjct: 61  SDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVVN 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRGYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLR YIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PSYAERFIRKHFKRSS 197
           PSYAERFIR+HFKR S
Sbjct: 181 PSYAERFIREHFKRGS 196

[Close]

BLAST of Lag0036992 vs. NCBI nr
Match: KAG6608449.1 (Protein DCL-like, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 355.9 bits (912), Expect = 2.2e-94
Identity = 171/196 (87.24%), Postives = 180/196 (91.84%), Query Frame = 0


Query: 1   MATSLLIRGHPLLRLALRQCGLCTGIVQVARRSCCTATAASTPPDGNLTSAENTTSVLSA 60
           M  SLL+RGHPL+RL LR  GLCTGIVQV RRSCCTA  ASTPP G L+SAENTTSVLSA
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCTGIVQVTRRSCCTAMPASTPPGGGLSSAENTTSVLSA 60

Query: 61  SDPPKYQRWDEPDYRKWKNQEKEILSDIEPIISLTKEILHSNRYVDGERLTSEVERIVVD 120
           +DPPKYQRWDEP YRKWKNQE+EILSDI+P+ISLTKEILHSNRYVDGERLT E E+IVVD
Sbjct: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRGYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLR YIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PSYAERFIRKHFKRSS 197
           P YAERFIR+HFKR S
Sbjct: 181 PLYAERFIREHFKRGS 196

[Close]

BLAST of Lag0036992 vs. NCBI nr
Match: KAG7037785.1 (Protein DCL-like, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 354.0 bits (907), Expect = 8.4e-94
Identity = 170/194 (87.63%), Postives = 179/194 (92.27%), Query Frame = 0


Query: 1   MATSLLIRGHPLLRLALRQCGLCTGIVQVARRSCCTATAASTPPDGNLTSAENTTSVLSA 60
           M  SLL+RGHPL+RL LR  GLCTGIVQV RRSCCTA  ASTPP G L+SAENTTSVLSA
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCTGIVQVTRRSCCTAMPASTPPGGGLSSAENTTSVLSA 60

Query: 61  SDPPKYQRWDEPDYRKWKNQEKEILSDIEPIISLTKEILHSNRYVDGERLTSEVERIVVD 120
           +DPPKYQRWDEP YRKWKNQE+EILSDI+P+ISLTKEILHSNRYVDGERLT E E+IVVD
Sbjct: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRGYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLR YIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PSYAERFIRKHFKR 195
           P YAERFIR+HFKR
Sbjct: 181 PLYAERFIREHFKR 194

[Close]

BLAST of Lag0036992 vs. NCBI nr
Match: XP_022940238.1 (protein DCL homolog, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 352.8 bits (904), Expect = 1.9e-93
Identity = 170/196 (86.73%), Postives = 179/196 (91.33%), Query Frame = 0


Query: 1   MATSLLIRGHPLLRLALRQCGLCTGIVQVARRSCCTATAASTPPDGNLTSAENTTSVLSA 60
           M  SLL+RGHPL+RL LR  GLC GIVQV RRSCCTA  ASTPP G L+SAENTTSVLSA
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCAGIVQVTRRSCCTAVPASTPPGGGLSSAENTTSVLSA 60

Query: 61  SDPPKYQRWDEPDYRKWKNQEKEILSDIEPIISLTKEILHSNRYVDGERLTSEVERIVVD 120
           +DPPKYQRW+EP YRKWKNQE+EILSDI+PIISLTKEILHSNRYVDGERLT E E+IVVD
Sbjct: 61  TDPPKYQRWNEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRGYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLR YIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PSYAERFIRKHFKRSS 197
           P YAERFIR+HFKR S
Sbjct: 181 PLYAERFIREHFKRGS 196

[Close]

BLAST of Lag0036992 vs. NCBI nr
Match: XP_022982288.1 (protein DCL homolog, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 352.4 bits (903), Expect = 2.4e-93
Identity = 169/196 (86.22%), Postives = 179/196 (91.33%), Query Frame = 0


Query: 1   MATSLLIRGHPLLRLALRQCGLCTGIVQVARRSCCTATAASTPPDGNLTSAENTTSVLSA 60
           M  SLL+RGHPL+RL LR  GLCTGI+QV RRSCCTA  ASTPP G L+SAENTTSVLS 
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCTGILQVTRRSCCTAMPASTPPGGGLSSAENTTSVLST 60

Query: 61  SDPPKYQRWDEPDYRKWKNQEKEILSDIEPIISLTKEILHSNRYVDGERLTSEVERIVVD 120
           +DPPKYQRWDEP YRKWKNQE+EILSDI+PIISLTKEILHSNRYVDGERLT E E+IVVD
Sbjct: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRGYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLR YIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PSYAERFIRKHFKRSS 197
           P YA+RFIR+HFKR S
Sbjct: 181 PLYADRFIREHFKRGS 196

[Close]

BLAST of Lag0036992 vs. ExPASy Swiss-Prot
Match: Q9C642 (Protein DCL homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DCL PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 4.4e-29
Identity = 54/114 (47.37%), Postives = 79/114 (69.30%), Query Frame = 0


Query: 81  EKEILSDIEPIISLTKEILHSNRYVDGERLTSEVERIVVDRLLAHHPHAEDKIGCGLESI 140
           E +IL    P++   + ILHS +Y + +RL+ E ER +++ LL +HP  E KIGCG++ I
Sbjct: 101 EDKILEVTVPLVGFVRMILHSGKYANRDRLSPEHERTIIEMLLPYHPECEKKIGCGIDYI 160

Query: 141 MVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRGYIRNKYPSYAERFIRKHFKR 195
           MV  HP F  SRC+F++R DG  +DFSY KC++G I+ KYP YA+ FI +HF++
Sbjct: 161 MVGHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLIKKKYPLYADSFILRHFRK 214

[Close]

BLAST of Lag0036992 vs. ExPASy Swiss-Prot
Match: Q42463 (Protein DCL, chloroplastic OS=Solanum lycopersicum OX=4081 GN=DCL PE=2 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 2.9e-28
Identity = 60/146 (41.10%), Postives = 91/146 (62.33%), Query Frame = 0


Query: 49  TSAENTTSVLSASDPPKYQRWDEPDYRKWKNQEKEILSDIEPIISLTKEILHSNRYVDGE 108
           T++E+   V   SD    ++    D   W + E +IL D  P++   + ILHS +Y  G+
Sbjct: 76  TTSESEELVKEESDDEVGKK--SGDGEGWVDWEDQILEDTVPLVGFVRMILHSGKYAIGD 135

Query: 109 RLTSEVERIVVDRLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSY 168
           RL+ + +R ++ RLL +HP  + KIG G++ I V  HP F +SRCLF++R DG  +DFSY
Sbjct: 136 RLSPDHQRTILQRLLPYHPECDKKIGPGVDYITVGYHPDFENSRCLFIVRKDGETVDFSY 195

Query: 169 QKCLRGYIRNKYPSYAERFIRKHFKR 195
            KC++G IR  YP YA+ FI +HF++
Sbjct: 196 WKCIKGLIRKNYPLYADSFILRHFRK 219

[Close]

BLAST of Lag0036992 vs. ExPASy Swiss-Prot
Match: Q5D869 (DNA-directed RNA polymerase V subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPE1 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.5e-24
Identity = 49/116 (42.24%), Postives = 78/116 (67.24%), Query Frame = 0


Query: 79   NQEKEILSDIEPIISLTKEILHSNRYVDGERLTSEVERIVVDRLLAHHPHAEDKIGCGLE 138
            ++E+E+LSD+EP++   ++I+H + Y DG+ ++ + +  V++++L  HP  E K+G G++
Sbjct: 1734 SEEQELLSDVEPVMRTLRKIMHPSAYPDGDPISDDDKTFVLEKILNFHPQKETKLGSGVD 1793

Query: 139  SIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRGYIRNKYPSYAERFIRKHFKR 195
             I VD+H  F  SRC FV+ TDG   DFSY+K L  Y+  KYP  AE FI K+F +
Sbjct: 1794 FITVDKHTIFSDSRCFFVVSTDGAKQDFSYRKSLNNYLMKKYPDRAEEFIDKYFTK 1849

[Close]

BLAST of Lag0036992 vs. ExPASy Swiss-Prot
Match: Q9LQ02 (DNA-directed RNA polymerase IV subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPD1 PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 4.0e-06
Identity = 38/120 (31.67%), Postives = 58/120 (48.33%), Query Frame = 0


Query: 77   WKNQEKEILSDIEPIISLTKEILHSNRYVDGERLTSEVERIVVDRLLAHHPHAEDKIGCG 136
            WKN        IE +    K ILHS    +   L +E +  +V  +L  HP++ +KIG G
Sbjct: 1336 WKN--------IELLSQSLKRILHS---YEINELLNERDEGLVKMVLQLHPNSVEKIGPG 1395

Query: 137  LESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRGYIRNKYPSYAERFIRKHFKRSS 196
            ++ I V +  +   S C  V+R DG + DFSY KC+ G  +   P     +  K+ K  +
Sbjct: 1396 VKGIRVAK-SKHGDSCCFEVVRIDGTFEDFSYHKCVLGATKIIAPKKMNFYKSKYLKNGT 1443

[Close]

BLAST of Lag0036992 vs. ExPASy TrEMBL
Match: A0A6J1C4J4 (protein DCL, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007322 PE=4 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 3.9e-97
Identity = 176/196 (89.80%), Postives = 184/196 (93.88%), Query Frame = 0


Query: 1   MATSLLIRGHPLLRLALRQCGLCTGIVQVARRSCCTATAASTPPDGNLTSAENTTSVLSA 60
           MA SLL+RGHPLLRL LR  GLCTGIVQV RRSCCTATAA TPPDGNLTSAEN TSVLS+
Sbjct: 1   MAASLLLRGHPLLRLGLRHRGLCTGIVQVTRRSCCTATAAPTPPDGNLTSAENITSVLSS 60

Query: 61  SDPPKYQRWDEPDYRKWKNQEKEILSDIEPIISLTKEILHSNRYVDGERLTSEVERIVVD 120
           SDPPKY RWDEPDYRKWK+QE+E+L+DIEPIISLTKEILHSNRYVDGERLTS  ERIVV+
Sbjct: 61  SDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVVN 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRGYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLR YIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PSYAERFIRKHFKRSS 197
           PSYAERFIR+HFKR S
Sbjct: 181 PSYAERFIREHFKRGS 196

[Close]

BLAST of Lag0036992 vs. ExPASy TrEMBL
Match: A0A6J1FNQ4 (protein DCL homolog, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111445919 PE=4 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 9.1e-94
Identity = 170/196 (86.73%), Postives = 179/196 (91.33%), Query Frame = 0


Query: 1   MATSLLIRGHPLLRLALRQCGLCTGIVQVARRSCCTATAASTPPDGNLTSAENTTSVLSA 60
           M  SLL+RGHPL+RL LR  GLC GIVQV RRSCCTA  ASTPP G L+SAENTTSVLSA
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCAGIVQVTRRSCCTAVPASTPPGGGLSSAENTTSVLSA 60

Query: 61  SDPPKYQRWDEPDYRKWKNQEKEILSDIEPIISLTKEILHSNRYVDGERLTSEVERIVVD 120
           +DPPKYQRW+EP YRKWKNQE+EILSDI+PIISLTKEILHSNRYVDGERLT E E+IVVD
Sbjct: 61  TDPPKYQRWNEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRGYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLR YIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PSYAERFIRKHFKRSS 197
           P YAERFIR+HFKR S
Sbjct: 181 PLYAERFIREHFKRGS 196

[Close]

BLAST of Lag0036992 vs. ExPASy TrEMBL
Match: A0A6J1J4G4 (protein DCL homolog, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481165 PE=4 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 1.2e-93
Identity = 169/196 (86.22%), Postives = 179/196 (91.33%), Query Frame = 0


Query: 1   MATSLLIRGHPLLRLALRQCGLCTGIVQVARRSCCTATAASTPPDGNLTSAENTTSVLSA 60
           M  SLL+RGHPL+RL LR  GLCTGI+QV RRSCCTA  ASTPP G L+SAENTTSVLS 
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCTGILQVTRRSCCTAMPASTPPGGGLSSAENTTSVLST 60

Query: 61  SDPPKYQRWDEPDYRKWKNQEKEILSDIEPIISLTKEILHSNRYVDGERLTSEVERIVVD 120
           +DPPKYQRWDEP YRKWKNQE+EILSDI+PIISLTKEILHSNRYVDGERLT E E+IVVD
Sbjct: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRGYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLR YIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PSYAERFIRKHFKRSS 197
           P YA+RFIR+HFKR S
Sbjct: 181 PLYADRFIREHFKRGS 196

[Close]

BLAST of Lag0036992 vs. ExPASy TrEMBL
Match: A0A1S3BU88 (protein DCL, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493574 PE=4 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 7.2e-91
Identity = 162/196 (82.65%), Postives = 177/196 (90.31%), Query Frame = 0


Query: 1   MATSLLIRGHPLLRLALRQCGLCTGIVQVARRSCCTATAASTPPDGNLTSAENTTSVLSA 60
           MA S L RGHPLLRL L++ GLCTG+VQV RRSCC+AT ASTP DG+LT+ +N TS++S+
Sbjct: 1   MAASFLFRGHPLLRLGLKRRGLCTGVVQVTRRSCCSATVASTPSDGDLTAGDNATSLVSS 60

Query: 61  SDPPKYQRWDEPDYRKWKNQEKEILSDIEPIISLTKEILHSNRYVDGERLTSEVERIVVD 120
           S+PPKYQRWDEPDYRKWKNQE+EIL DIEPII LTKEILHS+RY DGERLT E ER VVD
Sbjct: 61  SEPPKYQRWDEPDYRKWKNQEEEILGDIEPIIFLTKEILHSHRYADGERLTLEDERTVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRGYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRC FVIRTDGGWIDFSYQKCLR YIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCFFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PSYAERFIRKHFKRSS 197
           PS+AERFIR+HFKR S
Sbjct: 181 PSFAERFIREHFKRGS 196

[Close]

BLAST of Lag0036992 vs. ExPASy TrEMBL
Match: A0A6J1IHJ1 (protein DCL homolog, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111477278 PE=4 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 2.1e-90
Identity = 165/196 (84.18%), Postives = 177/196 (90.31%), Query Frame = 0


Query: 1   MATSLLIRGHPLLRLALRQCGLCTGIVQVARRSCCTATAASTPPDGNLTSAENTTSVLSA 60
           MA SLL+RGHPLLR  L   GLC+G+VQV RRSCCTATAASTPPDG+LTSAENT SV SA
Sbjct: 1   MAASLLLRGHPLLRFGLWHRGLCSGVVQVTRRSCCTATAASTPPDGSLTSAENTASVSSA 60

Query: 61  SDPPKYQRWDEPDYRKWKNQEKEILSDIEPIISLTKEILHSNRYVDGERLTSEVERIVVD 120
           S   KYQRW EPDYRKWK+QE EILSD+EP++SLTKEILHSNRYVDGERLTSE ERIVVD
Sbjct: 61  SQSTKYQRWCEPDYRKWKDQEDEILSDVEPVMSLTKEILHSNRYVDGERLTSEDERIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRGYIRNKY 180
           RLLAHHPHAEDKIGCGLE IMVDRHPQFR+SRCLFV+RTDGGWIDFSYQKCLR YIR+KY
Sbjct: 121 RLLAHHPHAEDKIGCGLEFIMVDRHPQFRYSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY 180

Query: 181 PSYAERFIRKHFKRSS 197
           PSYAE FIR+HFKR S
Sbjct: 181 PSYAEMFIREHFKRGS 196

The following BLAST results are available for this feature:

BLAST of Lag0036992 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135338.1	8.1e-97	89.80	protein DCL, chloroplastic isoform X1 [Momordica charantia]	[more]
KAG6608449.1	2.2e-94	87.24	Protein DCL-like, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7037785.1	8.4e-94	87.63	Protein DCL-like, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosp...	[more]
XP_022940238.1	1.9e-93	86.73	protein DCL homolog, chloroplastic-like [Cucurbita moschata]	[more]
XP_022982288.1	2.4e-93	86.22	protein DCL homolog, chloroplastic-like isoform X1 [Cucurbita maxima]	[more]

BLAST of Lag0036992 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C642	4.4e-29	47.37	Protein DCL homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DCL PE=2 S...	[more]
Q42463	2.9e-28	41.10	Protein DCL, chloroplastic OS=Solanum lycopersicum OX=4081 GN=DCL PE=2 SV=1	[more]
Q5D869	1.5e-24	42.24	DNA-directed RNA polymerase V subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPE1...	[more]
Q9LQ02	4.0e-06	31.67	DNA-directed RNA polymerase IV subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPD...	[more]

BLAST of Lag0036992 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C4J4	3.9e-97	89.80	protein DCL, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC11100...	[more]
A0A6J1FNQ4	9.1e-94	86.73	protein DCL homolog, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1J4G4	1.2e-93	86.22	protein DCL homolog, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 G...	[more]
A0A1S3BU88	7.2e-91	82.65	protein DCL, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493574 PE=4 SV=1	[more]
A0A6J1IHJ1	2.1e-90	84.18	protein DCL homolog, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111477...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF11523	DUF3223	coord: 96..172 e-value: 2.3E-27 score: 95.5
None	No IPR available	GENE3D	3.10.450.40		coord: 82..177 e-value: 2.4E-23 score: 84.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 38..62
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 38..67
None	No IPR available	PANTHER	PTHR33415:SF4	DCL PROTEIN (DUF3223)	coord: 1..195
IPR044673	Protein DCL-like	PANTHER	PTHR33415	FAMILY NOT NAMED	coord: 1..195

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0036992.1	Lag0036992.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009658	chloroplast organization
biological_process	GO:1901259	chloroplast rRNA processing
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0003729	mRNA binding