Lag0036962 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0036962
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Lipase, class 3
Location	chr2: 2421921 .. 2424224 (-)
RNA-Seq Expression	Lag0036962
Synteny	Lag0036962

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTTCAAAGGAACATGAATTTAGTAAAAGTTATATGATACTGAAGCCTGAGAATGCAAATCTGTTGTATCTGTTTCTGTTCATACTACCATTTGGGTTCAGAAAGAGGAAGTTCATAGATTGCCCAGAGGGCAAAGAAGATTCATACAGAAGCTTTGCAAATCGATGGCTCATCTTCATCTCCATGCTGTTGCAGCTATTTCTTGGCGCCATCGCCGCTCCACTCGCAGCCTTGGACTCCATTTTCCTAAAGCTCTTCAACTTCATCTCCTTCAACGGCGGCATTCTTGGGCTTCTGTTTAAACTTGTCAAAAGTACTTTCAAATTTTCATCACTTTAATTTATCTCATACCGTCTATCTTATAAACTATGAGGTCTTTACTTATCTTTTCAACTATCTTTTTAACGTGGAATTTTCAACATAATTTTCTTTTTGCCTGCATCCATGGCGCAAACGATCTTTAATTTCTCACAGTTTTGTTTTTGTGTGTGCGTAGGAAAGCCTTTTGTGCAGCCTGACGAAAATTCCCCGGACTACAGATCGGTGCTCGGATTTACAGATTGGAGAAAAGATTTGGACTCGTCCATAAAACCCGATGACACTTTCAGATATTATGGTGCCCTAACTATGATGGCTGCCAAAGTCTCCTATGAATTCCTCCCATTTGTCCAATCTGTCGTAAATGACCATTGGAAGGTAAATATACTATTCAGGTCCTCTATTTTTAGTTTTGGTTAGGACATTATAATATCTGAAAGTGTCGTCGTGATTTATGATCTGATTGGTGTTAAAAGATAAGTTTATATCATATTGATTAAGGTTGTATTGTACTTGAACAAAAAAAAAACAGGGATGATAGTTGAACAACGACGGTGGGATCAAAATGATTATTTTTTTGAATAATTTTAATTAATAATTGGATAGCAAAATAAAATTTAAACCAAAATTTTAAATGTATATCCATCAATTAATAGCTGTATATGATTGTGACCATAGTTTTCGTGATTTTTGAATACTCCAAATGGTGACGATGTTTTCTTCTTCATGTTGCAGATGAAGATGCTCCATTTCTACGACTTTTGGAGTGGTAGAGAATTTGACATCTTAATTGACGGTCTTAATTCCTATACTTTTAAATATTTTAAATTGATTTTTCTTTGATTTAATCAAATCTCAATATTGTTACAATATACAATTTTTTATGTGACAATGAGTTTATGTTTTCTAATTTACTATGTTACGTACTTGTAATGATTTAATGTTGAGTAAAAATATAGAAGTACTGATGAGGGTGAGACTTTTGTGTGAGTTTAACCCTGTAAAAATAATTTGTGAAGTTTAACAAAATCATAGGGACAAATTAGTAATTTCAACTTTGTAATTCTTTTTCTTTTGCCAACAAGAGCTTAGCTCAACTGACATAAAGATCTATTGATAACCATGAGGTATGTTTGTTTGAGCCCGTTTGATCATAACACTCTGATTTTGATTTTTTAAATTTAAGCTTACAAACATTACTTTACTTTTTAAATATGTCCTCAAAATCCAGGTAAAATCTTAAAAGCATAATAAAGCAATTTTTAAAAACTTGATTTTGTTGTTTAAAAAAAAAACATGATTTAACTTTGTTTTTGAAATATTTCTAAGAATACTAACGTTTCATAGTTAAGAAAATATAATATAAGGAATTAACCATCAAAATTTTAAAAAATAGAAAAGAAAACAAAATGATATATACAAAACCGGGTACTGATAACCATTTTTAAAATTTTCTAACATGTTTATAAAACTGTAAATAAATTATCAAAACGAAACATGGATATTTCGTTGTTCATGCAAAAGTTTTAGGATCGTGTAAAAGTAAACCCTAATTTATTTTAAATCTTTTCAGACTTTCAGAATAAAACTACGACACAAGGCTTTATATTTCAGAACATAGCAACAGACCCAAACATAACAGTGATAGCATTCCGAGGCACCTCTCCCCTGGACGCCTACGACTGGCAAGTGGATGCGGACTTTTCTTGGTACGAAATTGAAGGCATTGGTCGAATCCACAGCGGCTTCATGAAAGCCCTTGGCCTACAAAAGGTCACAGGCTGGCCCAAGAACCTCCCTCCAAGCCCTGACAATCGCCAATTTGCCTACTACACCCTTCGCCAACAGCTCAGAGACATTGCCAAAGGCAACGACAAGGCGAGGTTCTGTTGGAAACGAAGTAGGCCCTCACAATACAACTCAAGAGATAAGAGAAGAAGATTGTTCTCTCACTCACACAAGATTGGAGAAACTAGAACTTGA

mRNA sequence

ATGATTTCAAAGGAACATGAATTTAGTAAAAGTTATATGATACTGAAGCCTGAGAATGCAAATCTGTTGTATCTGTTTCTGTTCATACTACCATTTGGGTTCAGAAAGAGGAAGTTCATAGATTGCCCAGAGGGCAAAGAAGATTCATACAGAAGCTTTGCAAATCGATGGCTCATCTTCATCTCCATGCTGTTGCAGCTATTTCTTGGCGCCATCGCCGCTCCACTCGCAGCCTTGGACTCCATTTTCCTAAAGCTCTTCAACTTCATCTCCTTCAACGGCGGCATTCTTGGGCTTCTGTTTAAACTTGTCAAAAGAAAGCCTTTTGTGCAGCCTGACGAAAATTCCCCGGACTACAGATCGGTGCTCGGATTTACAGATTGGAGAAAAGATTTGGACTCGTCCATAAAACCCGATGACACTTTCAGATATTATGGTGCCCTAACTATGATGGCTGCCAAAGTCTCCTATGAATTCCTCCCATTTGTCCAATCTGTCGTAAATGACCATTGGAAGATGAAGATGCTCCATTTCTACGACTTTTGGAGTGGTAGAGAATTTGACATCTTAATTGACGACTTTCAGAATAAAACTACGACACAAGGCTTTATATTTCAGAACATAGCAACAGACCCAAACATAACAGTGATAGCATTCCGAGGCACCTCTCCCCTGGACGCCTACGACTGGCAAGTGGATGCGGACTTTTCTTGGTACGAAATTGAAGGCATTGGTCGAATCCACAGCGGCTTCATGAAAGCCCTTGGCCTACAAAAGGTCACAGGCTGGCCCAAGAACCTCCCTCCAAGCCCTGACAATCGCCAATTTGCCTACTACACCCTTCGCCAACAGCTCAGAGACATTGCCAAAGGCAACGACAAGGCGAGGTTCTGTTGGAAACGAAGTAGGCCCTCACAATACAACTCAAGAGATAAGAGAAGAAGATTGTTCTCTCACTCACACAAGATTGGAGAAACTAGAACTTGA

Coding sequence (CDS)

ATGATTTCAAAGGAACATGAATTTAGTAAAAGTTATATGATACTGAAGCCTGAGAATGCAAATCTGTTGTATCTGTTTCTGTTCATACTACCATTTGGGTTCAGAAAGAGGAAGTTCATAGATTGCCCAGAGGGCAAAGAAGATTCATACAGAAGCTTTGCAAATCGATGGCTCATCTTCATCTCCATGCTGTTGCAGCTATTTCTTGGCGCCATCGCCGCTCCACTCGCAGCCTTGGACTCCATTTTCCTAAAGCTCTTCAACTTCATCTCCTTCAACGGCGGCATTCTTGGGCTTCTGTTTAAACTTGTCAAAAGAAAGCCTTTTGTGCAGCCTGACGAAAATTCCCCGGACTACAGATCGGTGCTCGGATTTACAGATTGGAGAAAAGATTTGGACTCGTCCATAAAACCCGATGACACTTTCAGATATTATGGTGCCCTAACTATGATGGCTGCCAAAGTCTCCTATGAATTCCTCCCATTTGTCCAATCTGTCGTAAATGACCATTGGAAGATGAAGATGCTCCATTTCTACGACTTTTGGAGTGGTAGAGAATTTGACATCTTAATTGACGACTTTCAGAATAAAACTACGACACAAGGCTTTATATTTCAGAACATAGCAACAGACCCAAACATAACAGTGATAGCATTCCGAGGCACCTCTCCCCTGGACGCCTACGACTGGCAAGTGGATGCGGACTTTTCTTGGTACGAAATTGAAGGCATTGGTCGAATCCACAGCGGCTTCATGAAAGCCCTTGGCCTACAAAAGGTCACAGGCTGGCCCAAGAACCTCCCTCCAAGCCCTGACAATCGCCAATTTGCCTACTACACCCTTCGCCAACAGCTCAGAGACATTGCCAAAGGCAACGACAAGGCGAGGTTCTGTTGGAAACGAAGTAGGCCCTCACAATACAACTCAAGAGATAAGAGAAGAAGATTGTTCTCTCACTCACACAAGATTGGAGAAACTAGAACTTGA

Protein sequence

MISKEHEFSKSYMILKPENANLLYLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIFISMLLQLFLGAIAAPLAALDSIFLKLFNFISFNGGILGLLFKLVKRKPFVQPDENSPDYRSVLGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFVQSVVNDHWKMKMLHFYDFWSGREFDILIDDFQNKTTTQGFIFQNIATDPNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHSGFMKALGLQKVTGWPKNLPPSPDNRQFAYYTLRQQLRDIAKGNDKARFCWKRSRPSQYNSRDKRRRLFSHSHKIGETRT

Homology

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BLAST of Lag0036962 vs. NCBI nr
Match: XP_038899591.1 (triacylglycerol lipase OBL1-like [Benincasa hispida])

HSP 1 Score: 434.9 bits (1117), Expect = 6.3e-118
Identity = 209/293 (71.33%), Postives = 243/293 (82.94%), Query Frame = 0


Query: 5   EHEFSKSYMILKPENANLLYLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIFISML 64
           E EFSK+Y+ILKPENANLL LFLF+LPFG RKRKFIDCP GKED +++F +RWLIFISML
Sbjct: 5   ESEFSKNYVILKPENANLLDLFLFLLPFGLRKRKFIDCPLGKEDPFKNFRDRWLIFISML 64

Query: 65  LQLFLGAIAAPLAALDSIFLKLFNFISFNGGILGLLFKLVKRKPFVQPDENSPDYRSVLG 124
           LQ  L  IA PLA LDS  LKL NFISFNGG+ G+L K++K K F++ +E+SP+Y SV+G
Sbjct: 65  LQFILAIIATPLAKLDSFLLKLLNFISFNGGVSGILSKILKGKSFLEVNEDSPEYTSVVG 124

Query: 125 FTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFVQSVVNDHWKMKMLHFYDFWSG 184
           F DWR+DLDSSIKPDDTFRYY ALT+MAAKVSYE  PFVQSVVND WKMK+L +++FW  
Sbjct: 125 FADWRRDLDSSIKPDDTFRYYSALTVMAAKVSYESKPFVQSVVNDRWKMKLLGYFNFW-- 184

Query: 185 REFDILIDDFQNKTTTQGFIFQNIATDPNITVIAFRGTSPLDAYDWQVDADFSWYEIEGI 244
                  +DFQ K TTQ F+F+N A DPN+ V+AFRGTS LD+YDWQVDADFSWYEIEG+
Sbjct: 185 -------NDFQGKATTQAFMFENTAIDPNVIVVAFRGTSLLDSYDWQVDADFSWYEIEGV 244

Query: 245 GRIHSGFMKALGLQKVTGWPKNLPPSPDNRQFAYYTLRQQLRDIAKGNDKARF 298
           GRIHSGFMKALGLQK TGWPK LP S   ++FAYYT+RQ+LRDIAK NDKARF
Sbjct: 245 GRIHSGFMKALGLQKATGWPKELPKS-QLQEFAYYTIRQKLRDIAKTNDKARF 287

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BLAST of Lag0036962 vs. NCBI nr
Match: XP_022139642.1 (uncharacterized protein LOC111010491 [Momordica charantia])

HSP 1 Score: 426.8 bits (1096), Expect = 1.7e-115
Identity = 208/299 (69.57%), Postives = 244/299 (81.61%), Query Frame = 0


Query: 1   MISKEHEFSKSYMILKPENANLLYLFLFILPFGFRKR-KFIDCPEGKEDSY-RSFANRWL 60
           M SKEHEFSK+Y+ILKP+NAN++ LFLF+LPFGFRKR KFIDCPEGKEDSY  SF NRWL
Sbjct: 1   MASKEHEFSKNYLILKPQNANIVDLFLFLLPFGFRKRTKFIDCPEGKEDSYTSSFTNRWL 60

Query: 61  IFISMLLQLFLGAIAAPLAALDSIFLKLFNFISFNGGILGLLFKLVKRKPFVQPDENSPD 120
           IFIS++LQ+ L AIA PLA LD+  L +FNFISFNGGILGLL +++K +  V+PDENS +
Sbjct: 61  IFISIVLQIILLAIATPLAKLDAFLLNVFNFISFNGGILGLLSRILKGESLVKPDENSAE 120

Query: 121 YRSVLGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFVQSVVNDHWKMKMLHF 180
           Y SV+GF DWR+DLD SIK ++ FRYYG LT+MAA++SYE  PFV SVVND WKMK+L  
Sbjct: 121 YTSVVGFADWRRDLDKSIKAEEIFRYYGGLTVMAAQLSYESKPFVLSVVNDRWKMKLLGH 180

Query: 181 YDFWSGREFDILIDDFQNKTTTQGFIFQNIATDPNITVIAFRGTSPLDAYDWQVDADFSW 240
           ++FW         +DFQ K TTQ F+FQN A DPN+TV+AFRGTSPLDAYDWQVDADFSW
Sbjct: 181 FNFW---------NDFQGKATTQAFMFQNTAADPNVTVVAFRGTSPLDAYDWQVDADFSW 240

Query: 241 YEIEGIGRIHSGFMKALGLQKVTGWPKNLPPSPDNRQFAYYTLRQQLRDIAKGNDKARF 298
           Y+IEG+GRIHSGFMKALGLQK TGWP+ L  S     FAYYTLRQ+LRDIAK ND A+F
Sbjct: 241 YDIEGVGRIHSGFMKALGLQKATGWPEELVKS--KHHFAYYTLRQKLRDIAKSNDNAKF 288

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BLAST of Lag0036962 vs. NCBI nr
Match: XP_022975964.1 (uncharacterized protein LOC111476506 isoform X2 [Cucurbita maxima])

HSP 1 Score: 426.4 bits (1095), Expect = 2.2e-115
Identity = 205/298 (68.79%), Postives = 241/298 (80.87%), Query Frame = 0


Query: 1   MISKEHEFSKSYMILKPENANLLYLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
           M  KEHEFSK+Y+ILKP NANL  LFLF+LPFGF+KRK +DCP+  EDSY SFA+R +IF
Sbjct: 1   MAWKEHEFSKTYVILKPHNANLFDLFLFLLPFGFKKRKLMDCPDANEDSYTSFADRLMIF 60

Query: 61  ISMLLQLFLGAIAAPLAALDSIFLKLFNFISFNGGILGLLFKLVKRKPFVQPDENSPDYR 120
           +S+LLQ+F+  IA PLA LD    KLFNF+SFNGGIL L  KL++ +  VQPD+NSPDY 
Sbjct: 61  VSLLLQIFILTIATPLAYLDVFLQKLFNFVSFNGGILRLFIKLMRGETLVQPDKNSPDYT 120

Query: 121 SVLGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFVQSVVNDHWKMKMLHFYD 180
           SV+GFTDWR+DLDSSIKP+DTFRYY ALT+MA+K+SYE LPFV+SVVND WKMK+L +YD
Sbjct: 121 SVVGFTDWRRDLDSSIKPNDTFRYYSALTVMASKLSYESLPFVESVVNDRWKMKLLGYYD 180

Query: 181 FWSGREFDILIDDFQNKTTTQGFIFQNIATDPNITVIAFRGTSPLDAYDWQVDADFSWYE 240
           FW         +DFQN+ TTQ F+FQN A+DPNI V+AFRGTSPLDA+DWQV+ DFSWY+
Sbjct: 181 FW---------NDFQNRATTQAFMFQNTASDPNIIVVAFRGTSPLDAFDWQVNVDFSWYD 240

Query: 241 IEGIGRIHSGFMKALGLQKVTGWPKNLPPS-PDNRQFAYYTLRQQLRDIAKGNDKARF 298
           I G+GRIHSGFMKALGLQK  GWPK L PS     QFAYYTLRQ+L DIAK N  ARF
Sbjct: 241 IHGVGRIHSGFMKALGLQKRKGWPKELIPSTTTTTQFAYYTLRQKLIDIAKSNANARF 289

[Close]

BLAST of Lag0036962 vs. NCBI nr
Match: XP_022975962.1 (uncharacterized protein LOC111476506 isoform X1 [Cucurbita maxima])

HSP 1 Score: 426.4 bits (1095), Expect = 2.2e-115
Identity = 205/298 (68.79%), Postives = 241/298 (80.87%), Query Frame = 0


Query: 1   MISKEHEFSKSYMILKPENANLLYLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
           M  KEHEFSK+Y+ILKP NANL  LFLF+LPFGF+KRK +DCP+  EDSY SFA+R +IF
Sbjct: 1   MAWKEHEFSKTYVILKPHNANLFDLFLFLLPFGFKKRKLMDCPDANEDSYTSFADRLMIF 60

Query: 61  ISMLLQLFLGAIAAPLAALDSIFLKLFNFISFNGGILGLLFKLVKRKPFVQPDENSPDYR 120
           +S+LLQ+F+  IA PLA LD    KLFNF+SFNGGIL L  KL++ +  VQPD+NSPDY 
Sbjct: 61  VSLLLQIFILTIATPLAYLDVFLQKLFNFVSFNGGILRLFIKLMRGETLVQPDKNSPDYT 120

Query: 121 SVLGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFVQSVVNDHWKMKMLHFYD 180
           SV+GFTDWR+DLDSSIKP+DTFRYY ALT+MA+K+SYE LPFV+SVVND WKMK+L +YD
Sbjct: 121 SVVGFTDWRRDLDSSIKPNDTFRYYSALTVMASKLSYESLPFVESVVNDRWKMKLLGYYD 180

Query: 181 FWSGREFDILIDDFQNKTTTQGFIFQNIATDPNITVIAFRGTSPLDAYDWQVDADFSWYE 240
           FW         +DFQN+ TTQ F+FQN A+DPNI V+AFRGTSPLDA+DWQV+ DFSWY+
Sbjct: 181 FW---------NDFQNRATTQAFMFQNTASDPNIIVVAFRGTSPLDAFDWQVNVDFSWYD 240

Query: 241 IEGIGRIHSGFMKALGLQKVTGWPKNLPPS-PDNRQFAYYTLRQQLRDIAKGNDKARF 298
           I G+GRIHSGFMKALGLQK  GWPK L PS     QFAYYTLRQ+L DIAK N  ARF
Sbjct: 241 IHGVGRIHSGFMKALGLQKRKGWPKELIPSTTTTTQFAYYTLRQKLIDIAKSNANARF 289

[Close]

BLAST of Lag0036962 vs. NCBI nr
Match: XP_022936589.1 (uncharacterized protein LOC111443148 isoform X1 [Cucurbita moschata] >XP_022936590.1 uncharacterized protein LOC111443148 isoform X1 [Cucurbita moschata])

HSP 1 Score: 424.9 bits (1091), Expect = 6.5e-115
Identity = 205/298 (68.79%), Postives = 241/298 (80.87%), Query Frame = 0


Query: 1   MISKEHEFSKSYMILKPENANLLYLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
           M  KEHEFSK+Y+ILKP NANL  LFLF+LPFGF+KRK +DCP+  EDSY SFA+R +IF
Sbjct: 1   MAWKEHEFSKNYVILKPHNANLFDLFLFLLPFGFKKRKLMDCPDANEDSYTSFADRLIIF 60

Query: 61  ISMLLQLFLGAIAAPLAALDSIFLKLFNFISFNGGILGLLFKLVKRKPFVQPDENSPDYR 120
           +S+LLQ+F+ AIA PLA LD    KLFNF+SFNGGI  L  KL++ +  VQPD+NSPDY 
Sbjct: 61  VSLLLQIFILAIATPLAYLDVFLQKLFNFVSFNGGIPRLFIKLMRGETLVQPDKNSPDYT 120

Query: 121 SVLGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFVQSVVNDHWKMKMLHFYD 180
           SV+GFTDWR+DLDSSIKP+DTFRYY ALT+MA+K+SYE LPFV+SVVND WKMK+L +YD
Sbjct: 121 SVVGFTDWRRDLDSSIKPNDTFRYYSALTVMASKLSYESLPFVESVVNDRWKMKLLGYYD 180

Query: 181 FWSGREFDILIDDFQNKTTTQGFIFQNIATDPNITVIAFRGTSPLDAYDWQVDADFSWYE 240
           FW         +DFQN+ TTQ F+FQN A+DPNI V+AFRGTSPLDA+DWQV+ DFSWY+
Sbjct: 181 FW---------NDFQNRATTQAFMFQNTASDPNIIVVAFRGTSPLDAFDWQVNVDFSWYD 240

Query: 241 IEGIGRIHSGFMKALGLQKVTGWPKNLPPS-PDNRQFAYYTLRQQLRDIAKGNDKARF 298
           I G+GRIHSGFMKALGLQK  GWPK L PS     QFAYYTLRQ+L DIAK N  ARF
Sbjct: 241 IHGVGRIHSGFMKALGLQKRKGWPKELIPSTTTTTQFAYYTLRQKLIDIAKSNADARF 289

[Close]

BLAST of Lag0036962 vs. ExPASy Swiss-Prot
Match: F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 3.4e-34
Identity = 96/326 (29.45%), Postives = 159/326 (48.77%), Query Frame = 0


Query: 11  SYMILKPENANLLYLFLF-ILPFGFRKRKFIDCPEGKEDS-----YRSFANRWLIFISML 70
           +Y+I++P     + LF + +      K KF++ P+ +E S       +  +RW+I +S+L
Sbjct: 18  NYLIVRPHRGGYIDLFRYGVRDDQTSKAKFLEMPDNREWSTITIDEEAEDHRWVIVVSIL 77

Query: 71  LQLFLGAIAAPLAALDSIFLKLFNFISFNGGILGLLFKLVKRKPFVQPDENSPDYRSVLG 130
           ++  +  +  P+     +     N  S NGG  GLL +L++ K  V P+  S  + S +G
Sbjct: 78  VRKIIRLLRTPMEFTGFVVDFFLNLFSANGGFFGLLLRLIQAK-VVIPERGSVTFVSTIG 137

Query: 131 FTDWRKDL-----------------DSSIKPDDTFRYYGALTMMAAKVSYEFLPFVQSVV 190
             D R  L                    +K +   R    L +MA+K++YE    V++VV
Sbjct: 138 QLDGRISLYKEWNFVEHLEGIDSVDSGRVKIELGSRGLMDLCVMASKLAYENAKVVENVV 197

Query: 191 NDHWKMKMLHFYDFWSGREFDILIDDFQNKTTTQGFIFQNIATDPNITVIAFRGTSPLDA 250
           + HWKM ++ F D W         +D+Q + +TQ F+F +   D N+ VI+FRGT P DA
Sbjct: 198 DLHWKMNLVEFLDCW---------NDYQKQMSTQVFVFTDKQKDANLIVISFRGTEPFDA 257

Query: 251 YDWQVDADFSWYEIEGIGRIHSGFMKALGL---QKVTGWPKNLPPSPDNRQ--------- 298
            DW  D D+SWYE+  +G++H GF++A+GL      T +  NL     + +         
Sbjct: 258 DDWGTDFDYSWYEVPNVGKLHMGFLEAMGLGNRDDTTTFHYNLFEQTSSEEENSKKNLLD 317

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BLAST of Lag0036962 vs. ExPASy Swiss-Prot
Match: A0A1S3ZP85 (Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 2.3e-30
Identity = 75/216 (34.72%), Postives = 116/216 (53.70%), Query Frame = 0


Query: 55  NRWLIFISMLLQLFLGAIAAPLAALDSIFLKLFNFISFNGGILGLLFKLVKRKPFVQPDE 114
           +RW+IF+S++++  +     P+     +     N  S NG  LGLL+ ++  K  V P  
Sbjct: 85  HRWVIFVSIIVRKLIAIFGKPMEWTGYLVEFFLNLFSLNGNFLGLLYNILHGK-VVMPHR 144

Query: 115 NSPDYRSVLGFTDWR------KDLDSSIKPDDTFRYYGA-------LTMMAAKVSYEFLP 174
            S  + S +G  D R      + L   I   D ++  G        L MMA+K++YE   
Sbjct: 145 GSETFISAIGHLDGRINLYKSETLTKEIGEPDFWQKIGIGHRDLMDLCMMASKLAYENEK 204

Query: 175 FVQSVVNDHWKMKMLHFYDFWSGREFDILIDDFQNKTTTQGFIFQNIATDPNITVIAFRG 234
            V++VVN HWKM  + FY+ W         +DF+ + +TQ F+  +   D N+ +++FRG
Sbjct: 205 VVRNVVNLHWKMHFVDFYNCW---------NDFEKEMSTQVFLLCDKPKDANLILVSFRG 264

Query: 235 TSPLDAYDWQVDADFSWYEIEGIGRIHSGFMKALGL 258
           T P DA DW  D D+SWYEI  +G++H GF++ALGL
Sbjct: 265 TEPFDADDWITDFDYSWYEIPKLGKVHMGFLEALGL 290

[Close]

BLAST of Lag0036962 vs. ExPASy Swiss-Prot
Match: Q5VKJ7 (Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 2.3e-22
Identity = 84/278 (30.22%), Postives = 126/278 (45.32%), Query Frame = 0


Query: 9   SKSYMILKPENANLLYLFLFILPFGFRK-RKFIDCPEGKEDSYRSFANRW--LIFISMLL 68
           S S++I+ P+    L LF  I+        KF    + +  +  ++ +RW   +FI  +L
Sbjct: 9   STSHLIVSPDEGTFLDLFKHIVLSDLGSGAKFFRASDQRVPATAAYYSRWPVSVFICKIL 68

Query: 69  QLFLGAIAAPLAALDSIFLKLFNFISFNGGILGLLFKLVKRKPFVQPDENSPDYRSVLGF 128
           QLF      P A L  +   L NF   N G LG+L  +   +  + P     D+ S +G+
Sbjct: 69  QLF----QMPAAMLGHLTDFLLNFYYQNHGFLGILRNIFLIRLKI-PKRGEADFISTIGY 128

Query: 129 TDWRKDL------------------DSSIKPDDTFRYYGA--------LTMMAAKVSYEF 188
            D R DL                  + S+K     +  GA        L +MA+K++YE 
Sbjct: 129 LDSRMDLHGTPMVSHQADEVISNADNPSLKEGHNSKIKGALGNRSLMDLCIMASKLAYEN 188

Query: 189 LPFVQSVVNDHWKMKMLHFYDFWSGREFDILIDDFQNKTTTQGFIFQNIATDPNITVIAF 248
              V+ VV +HWKM   HF   + G  +      FQ+   T  FIF +   D N+ VI+F
Sbjct: 189 TKVVERVVAEHWKM---HFVADYGGMNY------FQDARNTHAFIFCDKPKDANLIVISF 248

Query: 249 RGTSPLDAYDWQVDADFSWYEIEGIGRIHSGFMKALGL 258
           RGT P    +W  D DFS   +   G +H GF++A+GL
Sbjct: 249 RGTGPFSIPNWCTDFDFSLVGLGDAGSVHVGFLEAMGL 272

[Close]

BLAST of Lag0036962 vs. ExPASy TrEMBL
Match: A0A6J1CEI9 (uncharacterized protein LOC111010491 OS=Momordica charantia OX=3673 GN=LOC111010491 PE=4 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 8.3e-116
Identity = 208/299 (69.57%), Postives = 244/299 (81.61%), Query Frame = 0


Query: 1   MISKEHEFSKSYMILKPENANLLYLFLFILPFGFRKR-KFIDCPEGKEDSY-RSFANRWL 60
           M SKEHEFSK+Y+ILKP+NAN++ LFLF+LPFGFRKR KFIDCPEGKEDSY  SF NRWL
Sbjct: 1   MASKEHEFSKNYLILKPQNANIVDLFLFLLPFGFRKRTKFIDCPEGKEDSYTSSFTNRWL 60

Query: 61  IFISMLLQLFLGAIAAPLAALDSIFLKLFNFISFNGGILGLLFKLVKRKPFVQPDENSPD 120
           IFIS++LQ+ L AIA PLA LD+  L +FNFISFNGGILGLL +++K +  V+PDENS +
Sbjct: 61  IFISIVLQIILLAIATPLAKLDAFLLNVFNFISFNGGILGLLSRILKGESLVKPDENSAE 120

Query: 121 YRSVLGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFVQSVVNDHWKMKMLHF 180
           Y SV+GF DWR+DLD SIK ++ FRYYG LT+MAA++SYE  PFV SVVND WKMK+L  
Sbjct: 121 YTSVVGFADWRRDLDKSIKAEEIFRYYGGLTVMAAQLSYESKPFVLSVVNDRWKMKLLGH 180

Query: 181 YDFWSGREFDILIDDFQNKTTTQGFIFQNIATDPNITVIAFRGTSPLDAYDWQVDADFSW 240
           ++FW         +DFQ K TTQ F+FQN A DPN+TV+AFRGTSPLDAYDWQVDADFSW
Sbjct: 181 FNFW---------NDFQGKATTQAFMFQNTAADPNVTVVAFRGTSPLDAYDWQVDADFSW 240

Query: 241 YEIEGIGRIHSGFMKALGLQKVTGWPKNLPPSPDNRQFAYYTLRQQLRDIAKGNDKARF 298
           Y+IEG+GRIHSGFMKALGLQK TGWP+ L  S     FAYYTLRQ+LRDIAK ND A+F
Sbjct: 241 YDIEGVGRIHSGFMKALGLQKATGWPEELVKS--KHHFAYYTLRQKLRDIAKSNDNAKF 288

[Close]

BLAST of Lag0036962 vs. ExPASy TrEMBL
Match: A0A6J1IEH0 (uncharacterized protein LOC111476506 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111476506 PE=4 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 1.1e-115
Identity = 205/298 (68.79%), Postives = 241/298 (80.87%), Query Frame = 0


Query: 1   MISKEHEFSKSYMILKPENANLLYLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
           M  KEHEFSK+Y+ILKP NANL  LFLF+LPFGF+KRK +DCP+  EDSY SFA+R +IF
Sbjct: 1   MAWKEHEFSKTYVILKPHNANLFDLFLFLLPFGFKKRKLMDCPDANEDSYTSFADRLMIF 60

Query: 61  ISMLLQLFLGAIAAPLAALDSIFLKLFNFISFNGGILGLLFKLVKRKPFVQPDENSPDYR 120
           +S+LLQ+F+  IA PLA LD    KLFNF+SFNGGIL L  KL++ +  VQPD+NSPDY 
Sbjct: 61  VSLLLQIFILTIATPLAYLDVFLQKLFNFVSFNGGILRLFIKLMRGETLVQPDKNSPDYT 120

Query: 121 SVLGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFVQSVVNDHWKMKMLHFYD 180
           SV+GFTDWR+DLDSSIKP+DTFRYY ALT+MA+K+SYE LPFV+SVVND WKMK+L +YD
Sbjct: 121 SVVGFTDWRRDLDSSIKPNDTFRYYSALTVMASKLSYESLPFVESVVNDRWKMKLLGYYD 180

Query: 181 FWSGREFDILIDDFQNKTTTQGFIFQNIATDPNITVIAFRGTSPLDAYDWQVDADFSWYE 240
           FW         +DFQN+ TTQ F+FQN A+DPNI V+AFRGTSPLDA+DWQV+ DFSWY+
Sbjct: 181 FW---------NDFQNRATTQAFMFQNTASDPNIIVVAFRGTSPLDAFDWQVNVDFSWYD 240

Query: 241 IEGIGRIHSGFMKALGLQKVTGWPKNLPPS-PDNRQFAYYTLRQQLRDIAKGNDKARF 298
           I G+GRIHSGFMKALGLQK  GWPK L PS     QFAYYTLRQ+L DIAK N  ARF
Sbjct: 241 IHGVGRIHSGFMKALGLQKRKGWPKELIPSTTTTTQFAYYTLRQKLIDIAKSNANARF 289

[Close]

BLAST of Lag0036962 vs. ExPASy TrEMBL
Match: A0A6J1IM46 (uncharacterized protein LOC111476506 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111476506 PE=4 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 1.1e-115
Identity = 205/298 (68.79%), Postives = 241/298 (80.87%), Query Frame = 0


Query: 1   MISKEHEFSKSYMILKPENANLLYLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
           M  KEHEFSK+Y+ILKP NANL  LFLF+LPFGF+KRK +DCP+  EDSY SFA+R +IF
Sbjct: 1   MAWKEHEFSKTYVILKPHNANLFDLFLFLLPFGFKKRKLMDCPDANEDSYTSFADRLMIF 60

Query: 61  ISMLLQLFLGAIAAPLAALDSIFLKLFNFISFNGGILGLLFKLVKRKPFVQPDENSPDYR 120
           +S+LLQ+F+  IA PLA LD    KLFNF+SFNGGIL L  KL++ +  VQPD+NSPDY 
Sbjct: 61  VSLLLQIFILTIATPLAYLDVFLQKLFNFVSFNGGILRLFIKLMRGETLVQPDKNSPDYT 120

Query: 121 SVLGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFVQSVVNDHWKMKMLHFYD 180
           SV+GFTDWR+DLDSSIKP+DTFRYY ALT+MA+K+SYE LPFV+SVVND WKMK+L +YD
Sbjct: 121 SVVGFTDWRRDLDSSIKPNDTFRYYSALTVMASKLSYESLPFVESVVNDRWKMKLLGYYD 180

Query: 181 FWSGREFDILIDDFQNKTTTQGFIFQNIATDPNITVIAFRGTSPLDAYDWQVDADFSWYE 240
           FW         +DFQN+ TTQ F+FQN A+DPNI V+AFRGTSPLDA+DWQV+ DFSWY+
Sbjct: 181 FW---------NDFQNRATTQAFMFQNTASDPNIIVVAFRGTSPLDAFDWQVNVDFSWYD 240

Query: 241 IEGIGRIHSGFMKALGLQKVTGWPKNLPPS-PDNRQFAYYTLRQQLRDIAKGNDKARF 298
           I G+GRIHSGFMKALGLQK  GWPK L PS     QFAYYTLRQ+L DIAK N  ARF
Sbjct: 241 IHGVGRIHSGFMKALGLQKRKGWPKELIPSTTTTTQFAYYTLRQKLIDIAKSNANARF 289

[Close]

BLAST of Lag0036962 vs. ExPASy TrEMBL
Match: A0A6J1FE49 (uncharacterized protein LOC111443148 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443148 PE=4 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 3.1e-115
Identity = 205/298 (68.79%), Postives = 241/298 (80.87%), Query Frame = 0


Query: 1   MISKEHEFSKSYMILKPENANLLYLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
           M  KEHEFSK+Y+ILKP NANL  LFLF+LPFGF+KRK +DCP+  EDSY SFA+R +IF
Sbjct: 1   MAWKEHEFSKNYVILKPHNANLFDLFLFLLPFGFKKRKLMDCPDANEDSYTSFADRLIIF 60

Query: 61  ISMLLQLFLGAIAAPLAALDSIFLKLFNFISFNGGILGLLFKLVKRKPFVQPDENSPDYR 120
           +S+LLQ+F+ AIA PLA LD    KLFNF+SFNGGI  L  KL++ +  VQPD+NSPDY 
Sbjct: 61  VSLLLQIFILAIATPLAYLDVFLQKLFNFVSFNGGIPRLFIKLMRGETLVQPDKNSPDYT 120

Query: 121 SVLGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFVQSVVNDHWKMKMLHFYD 180
           SV+GFTDWR+DLDSSIKP+DTFRYY ALT+MA+K+SYE LPFV+SVVND WKMK+L +YD
Sbjct: 121 SVVGFTDWRRDLDSSIKPNDTFRYYSALTVMASKLSYESLPFVESVVNDRWKMKLLGYYD 180

Query: 181 FWSGREFDILIDDFQNKTTTQGFIFQNIATDPNITVIAFRGTSPLDAYDWQVDADFSWYE 240
           FW         +DFQN+ TTQ F+FQN A+DPNI V+AFRGTSPLDA+DWQV+ DFSWY+
Sbjct: 181 FW---------NDFQNRATTQAFMFQNTASDPNIIVVAFRGTSPLDAFDWQVNVDFSWYD 240

Query: 241 IEGIGRIHSGFMKALGLQKVTGWPKNLPPS-PDNRQFAYYTLRQQLRDIAKGNDKARF 298
           I G+GRIHSGFMKALGLQK  GWPK L PS     QFAYYTLRQ+L DIAK N  ARF
Sbjct: 241 IHGVGRIHSGFMKALGLQKRKGWPKELIPSTTTTTQFAYYTLRQKLIDIAKSNADARF 289

[Close]

BLAST of Lag0036962 vs. ExPASy TrEMBL
Match: A0A6J1F8V7 (uncharacterized protein LOC111443148 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111443148 PE=4 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 3.1e-115
Identity = 205/298 (68.79%), Postives = 241/298 (80.87%), Query Frame = 0


Query: 1   MISKEHEFSKSYMILKPENANLLYLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
           M  KEHEFSK+Y+ILKP NANL  LFLF+LPFGF+KRK +DCP+  EDSY SFA+R +IF
Sbjct: 1   MAWKEHEFSKNYVILKPHNANLFDLFLFLLPFGFKKRKLMDCPDANEDSYTSFADRLIIF 60

Query: 61  ISMLLQLFLGAIAAPLAALDSIFLKLFNFISFNGGILGLLFKLVKRKPFVQPDENSPDYR 120
           +S+LLQ+F+ AIA PLA LD    KLFNF+SFNGGI  L  KL++ +  VQPD+NSPDY 
Sbjct: 61  VSLLLQIFILAIATPLAYLDVFLQKLFNFVSFNGGIPRLFIKLMRGETLVQPDKNSPDYT 120

Query: 121 SVLGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFVQSVVNDHWKMKMLHFYD 180
           SV+GFTDWR+DLDSSIKP+DTFRYY ALT+MA+K+SYE LPFV+SVVND WKMK+L +YD
Sbjct: 121 SVVGFTDWRRDLDSSIKPNDTFRYYSALTVMASKLSYESLPFVESVVNDRWKMKLLGYYD 180

Query: 181 FWSGREFDILIDDFQNKTTTQGFIFQNIATDPNITVIAFRGTSPLDAYDWQVDADFSWYE 240
           FW         +DFQN+ TTQ F+FQN A+DPNI V+AFRGTSPLDA+DWQV+ DFSWY+
Sbjct: 181 FW---------NDFQNRATTQAFMFQNTASDPNIIVVAFRGTSPLDAFDWQVNVDFSWYD 240

Query: 241 IEGIGRIHSGFMKALGLQKVTGWPKNLPPS-PDNRQFAYYTLRQQLRDIAKGNDKARF 298
           I G+GRIHSGFMKALGLQK  GWPK L PS     QFAYYTLRQ+L DIAK N  ARF
Sbjct: 241 IHGVGRIHSGFMKALGLQKRKGWPKELIPSTTTTTQFAYYTLRQKLIDIAKSNADARF 289

[Close]

BLAST of Lag0036962 vs. TAIR 10
Match: AT1G45201.1 (triacylglycerol lipase-like 1 )

HSP 1 Score: 203.0 bits (515), Expect = 3.7e-52
Identity = 109/293 (37.20%), Postives = 168/293 (57.34%), Query Frame = 0


Query: 7   EFSKSYMILKPENANLLYLFLFILPFGFRKRKFIDCPEGKEDSY-RSFANRWLIFISMLL 66
           +F  SY ++ P  A+ L L L +        +FID P      + RSFA+RW++ +++ L
Sbjct: 7   KFCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWILALAIFL 66

Query: 67  QLFLGAIAAPLAALDSIFLKLFNFISFNGGILGLLFKLVKRKPFVQPDENSPDYRSVLGF 126
           Q  L  ++ P A +        N ++ NGG   L+  L+  K  V+PD++S  Y S +G 
Sbjct: 67  QKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGK-LVKPDKSSATYTSFIGC 126

Query: 127 TDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFVQSVVNDHWKMKMLHFYDFWSGR 186
           +D R +LD  I    +  Y   L++MA+K+SYE  P++ SVV + WKM ++  YDF++  
Sbjct: 127 SDRRIELDEKINV-GSIEYKSMLSIMASKISYESKPYITSVVKNTWKMDLVGNYDFYNA- 186

Query: 187 EFDILIDDFQNKTTTQGFIFQNIATDPNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIG 246
                   FQ    TQ F+F+  +T+P++ V++FRGT P +A DW  D D SWYE++ +G
Sbjct: 187 --------FQESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVG 246

Query: 247 RIHSGFMKALGLQKVTGWPK-NLPPSPDNRQFAYYTLRQQLRDIAKGNDKARF 298
           ++H+GF +ALGLQK  GWPK N+       Q+AYYT+RQ LRD    N   ++
Sbjct: 247 KVHAGFSRALGLQK-DGWPKENISLL---HQYAYYTIRQMLRDKLGRNKNLKY 284

[Close]

BLAST of Lag0036962 vs. TAIR 10
Match: AT1G45201.2 (triacylglycerol lipase-like 1 )

HSP 1 Score: 203.0 bits (515), Expect = 3.7e-52
Identity = 109/293 (37.20%), Postives = 168/293 (57.34%), Query Frame = 0


Query: 7   EFSKSYMILKPENANLLYLFLFILPFGFRKRKFIDCPEGKEDSY-RSFANRWLIFISMLL 66
           +F  SY ++ P  A+ L L L +        +FID P      + RSFA+RW++ +++ L
Sbjct: 7   KFCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWILALAIFL 66

Query: 67  QLFLGAIAAPLAALDSIFLKLFNFISFNGGILGLLFKLVKRKPFVQPDENSPDYRSVLGF 126
           Q  L  ++ P A +        N ++ NGG   L+  L+  K  V+PD++S  Y S +G 
Sbjct: 67  QKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGK-LVKPDKSSATYTSFIGC 126

Query: 127 TDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFVQSVVNDHWKMKMLHFYDFWSGR 186
           +D R +LD  I    +  Y   L++MA+K+SYE  P++ SVV + WKM ++  YDF++  
Sbjct: 127 SDRRIELDEKINV-GSIEYKSMLSIMASKISYESKPYITSVVKNTWKMDLVGNYDFYNA- 186

Query: 187 EFDILIDDFQNKTTTQGFIFQNIATDPNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIG 246
                   FQ    TQ F+F+  +T+P++ V++FRGT P +A DW  D D SWYE++ +G
Sbjct: 187 --------FQESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVG 246

Query: 247 RIHSGFMKALGLQKVTGWPK-NLPPSPDNRQFAYYTLRQQLRDIAKGNDKARF 298
           ++H+GF +ALGLQK  GWPK N+       Q+AYYT+RQ LRD    N   ++
Sbjct: 247 KVHAGFSRALGLQK-DGWPKENISLL---HQYAYYTIRQMLRDKLGRNKNLKY 284

[Close]

BLAST of Lag0036962 vs. TAIR 10
Match: AT5G42930.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 197.2 bits (500), Expect = 2.0e-50
Identity = 109/293 (37.20%), Postives = 168/293 (57.34%), Query Frame = 0


Query: 9   SKSYMILKPENANLLYLFLFILPFGFRKRKFIDCPEGK-EDSYRSFANRWLIFISMLLQL 68
           +K+Y +L P  A +  L   +       RKFI   E + ED    F  RW+IF+S+++Q 
Sbjct: 4   TKNYFVLDPREATVSDLIHLLFSSDLGDRKFIHSSEERIEDDLSRFRRRWIIFVSIVIQK 63

Query: 69  FLGAIAAPLAALDSIFLKLFNFISFNGGILGLLFKLVKRKPFVQPDENSPDYRSVLGFTD 128
            +     PL  +        N +S NGG L +L  L K    + P++ S  + S+ G  D
Sbjct: 64  LIILFKKPLYFVGFSLACWLNLLSSNGGFLMILPNLFKGN-IIWPEKASATFASLNGNLD 123

Query: 129 WRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFVQSVVNDHWKMKMLHFYDFWSGREF 188
            R +L+  ++     RY   L++MA+K+SYE + FV SV+++HWKM +L FY  W+G   
Sbjct: 124 RRVELNPKVERGSK-RYKAMLSIMASKLSYENINFVSSVLHNHWKMDLLGFYSCWNG--- 183

Query: 189 DILIDDFQNKTTTQGFIFQNIATDPNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRI 248
                 +Q + +T+  + ++ +TDPN+ +++FRGT P DA DW  D D SWYE++ +G+I
Sbjct: 184 ------YQKQRSTEVIVIKDTSTDPNLIIVSFRGTDPFDADDWCTDLDLSWYEVKNVGKI 243

Query: 249 HSGFMKALGLQKVTGWPK--NLPPSPD-NRQFAYYTLRQQLRDIAKGNDKARF 298
           H GFMKALGLQK  GWPK  NL  + +    +AYYT+R+ L++I   N  ++F
Sbjct: 244 HGGFMKALGLQK-EGWPKEVNLDETQNATTLYAYYTVRRHLKEILDQNPTSKF 284

[Close]

BLAST of Lag0036962 vs. TAIR 10
Match: AT5G67050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 180.3 bits (456), Expect = 2.6e-45
Identity = 106/304 (34.87%), Postives = 155/304 (50.99%), Query Frame = 0


Query: 1   MISKEHEFSKSYMILKPENANLLYLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
           M S + +  + Y+IL+PE      L   +      K + +D  E +E S+R   +RWLIF
Sbjct: 2   MNSDDDDEPRGYLILRPEELRPWELVRLLFSGDIEKPRSVDSSETEEHSFR---HRWLIF 61

Query: 61  ISMLLQLFLGAIAAPLAALDSIFLKLFNFISFNGGILGLLFKLVKRKPFVQPDENSPDYR 120
           +S++L   L   +  LA + S      NF+S N    GL      R   V P   S +Y+
Sbjct: 62  VSLVLLKLLRFFSKLLALVGSALEFSLNFLS-NNSFSGLFL----RGEVVMPQRTSENYQ 121

Query: 121 SVLGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFVQSVVNDHWKMKMLHFYD 180
           S +G  D R  LD ++  +D  +YY AL++MA+K++YE    ++ VV +HW MK L   D
Sbjct: 122 SFIGHLDTRVSLDMTLNREDGEKYYAALSIMASKIAYENAARIKHVVENHWNMKYLGLVD 181

Query: 181 FWSGREFDILIDDFQNKTTTQGFIFQNIAT------DPNITVIAFRGTSPLDAYDWQVDA 240
           +W         +++Q K TTQ FI     T           V+AFRGT   ++ DW  D 
Sbjct: 182 YW---------NEYQEKETTQAFIMSTDETTTRSNGQETTVVVAFRGTELFNSEDWCSDF 241

Query: 241 DFSWYEIEGIGRIHSGFMKALGLQKVTGWPKNLPPSPDNRQ-FAYYTLRQQLRDIAKGND 298
           D +W+E+  IG IH GFMKALGLQ    WPK    +PD +   AYY++R  L+ +   N 
Sbjct: 242 DITWFELPNIGNIHGGFMKALGLQNNCSWPKEPLSNPDRKSPLAYYSIRDSLKTLIAQNK 288

[Close]

BLAST of Lag0036962 vs. TAIR 10
Match: AT1G56630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 173.3 bits (438), Expect = 3.2e-43
Identity = 101/294 (34.35%), Postives = 158/294 (53.74%), Query Frame = 0


Query: 10  KSYMILKPENANLLYLFLFILPFGFRKRKFIDCP-EGKEDSYRSFANRWLIFISMLLQLF 69
           K+Y +L P  A +  L   +       RKF+D   E  E+    F  RW+IF+S+++Q  
Sbjct: 5   KNYFVLDPREATVTDLMRLLFYSDLENRKFVDTSVENLENRLCEFRGRWIIFVSIVVQKL 64

Query: 70  LGAIAAPLAALDSIFLKLFNFISFNGGILGLLFKLVKRKPFVQPDENSPDYRSVLGFTDW 129
           L  +  PL+ L        N  S NGG   +   LVK + F+ P++ S  + S+ G  D 
Sbjct: 65  LIILRKPLSFLGFALGFWLNLPSSNGGFFKIFLNLVKGR-FIWPEKTSATFASINGNLDQ 124

Query: 130 RKD--LDSSIKPDDTFRYYGALTMMAAKVSYEFLPFVQSVVNDHWKMKMLHFYDFWSGRE 189
           + +  L  SIK  D  RY   L++MA+K++YE   F++SV+ DHW+M +L FY       
Sbjct: 125 KVELGLGRSIKIGDE-RYKPLLSIMASKLAYENEHFIRSVLQDHWQMDLLGFYS------ 184

Query: 190 FDILIDDFQNKTTTQGFIFQNIATDPNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGR 249
                +DF    +T+  + ++   +PN+ V++FRGT P +A DW  D D SW+ +  +G+
Sbjct: 185 ---CPNDFDQTRSTEVIVIRDTKDNPNLIVVSFRGTDPFNADDWCTDLDLSWHNVMNVGK 244

Query: 250 IHSGFMKALGLQKVTGWPKNLPPSPDNR---QFAYYTLRQQLRDIAKGNDKARF 298
           IH GFMKALGL K  GW + +          Q AYYT+ +QL+++ + N  ++F
Sbjct: 245 IHGGFMKALGLPK-EGWYEEINSDQTQNKTSQLAYYTILRQLKEVFEQNPTSKF 286

The following BLAST results are available for this feature:

BLAST of Lag0036962 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038899591.1	6.3e-118	71.33	triacylglycerol lipase OBL1-like [Benincasa hispida]	[more]
XP_022139642.1	1.7e-115	69.57	uncharacterized protein LOC111010491 [Momordica charantia]	[more]
XP_022975964.1	2.2e-115	68.79	uncharacterized protein LOC111476506 isoform X2 [Cucurbita maxima]	[more]
XP_022975962.1	2.2e-115	68.79	uncharacterized protein LOC111476506 isoform X1 [Cucurbita maxima]	[more]
XP_022936589.1	6.5e-115	68.79	uncharacterized protein LOC111443148 isoform X1 [Cucurbita moschata] >XP_0229365...	[more]

BLAST of Lag0036962 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JFU8	3.4e-34	29.45	Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1	[more]
A0A1S3ZP85	2.3e-30	34.72	Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1	[more]
Q5VKJ7	2.3e-22	30.22	Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1	[more]

BLAST of Lag0036962 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CEI9	8.3e-116	69.57	uncharacterized protein LOC111010491 OS=Momordica charantia OX=3673 GN=LOC111010...	[more]
A0A6J1IEH0	1.1e-115	68.79	uncharacterized protein LOC111476506 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1IM46	1.1e-115	68.79	uncharacterized protein LOC111476506 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1FE49	3.1e-115	68.79	uncharacterized protein LOC111443148 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1F8V7	3.1e-115	68.79	uncharacterized protein LOC111443148 isoform X3 OS=Cucurbita moschata OX=3662 GN...	[more]

BLAST of Lag0036962 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G45201.1	3.7e-52	37.20	triacylglycerol lipase-like 1	[more]
AT1G45201.2	3.7e-52	37.20	triacylglycerol lipase-like 1	[more]
AT5G42930.1	2.0e-50	37.20	alpha/beta-Hydrolases superfamily protein	[more]
AT5G67050.1	2.6e-45	34.87	alpha/beta-Hydrolases superfamily protein	[more]
AT1G56630.1	3.2e-43	34.35	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 144..298 e-value: 4.6E-11 score: 44.6
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 151..256
None	No IPR available	PANTHER	PTHR46086:SF4	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 4..298
IPR044819	Triacylglycerol lipase OBL1-like	PANTHER	PTHR46086	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 4..298

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0036962.1	Lag0036962.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004806	triglyceride lipase activity