Lag0036955 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0036955
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionTrimethylguanosine synthase
Locationchr2: 2366543 .. 2372453 (+)
RNA-Seq ExpressionLag0036955
SyntenyLag0036955
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGATCTGGAAACGGAGAATCCGACGACGAAGTCGGAGCCTCTGCTATTAGGGCTCTCGGCTCTCTCTTCAAGTTGACCGAAGTCTTTCTCTGGTGACCTCTCTTTCTCACCTCCGTTCTCGCCGGAAGATTAGGGAAATCTAAATTAGAAGAAAACAGCTTATTTTACTTTCACAACAGTGTTCTGTTCGACTTTCTGCTTATTTTTCATTTCAGTTATTTGGTATTGACGAGTAGATTTGATGGCTGCATTACAGGGACGATGAGACAGAAACAGCTAGACGAGTGGAAAGCAGTGTAAGTTTCTAATTTCACTGTTTCGAATCACGGCATTGGCAATTCATTGAGTTCTCTTTACTCCCTCGTCACTTTCAAATATACTAATTTCTCTGTTTGTTTTAAAAAGTTAGCCCCTGATGCTGATGATACCAATAACGAAGAATTCGGGGAGAAAATCTGTTCTACAATCAGTGGCAAGTGCTTCATTCGTCTATCTCTTTGTTTGCCTGGCTTTTTTTTTTTTTTATTTCTAGTTATGAGATATTTGTAGCAAATTTTATGCTCACCCACTATTTCTGTGGCATAATTATGCGTGTTGGTTGGTGCTAAAATGTCAGCGGGATTTTATTTGGCCAGATATTAGCTTACAACCGGAGGATGTCGAACTTACTGAACAGATGAATGCTCTGGGGCTTCCTCTTTCGTTCCACACAAACAAAGAGGTACTTCGATACACTACTACTGTGAATTACTTAGGTTGAACTATGATACTGGTCCTGTACTCTTATGTTGGCACTCCTATACTTCTAGAATTGTATATTTTGGTTCATTAATTTCAACGGTTTCTATCTTCAGTGAATGTTAAAAGACGGTGAAGTTCACCGCCAAGTTAGTCTATCAATGTTGTGTCATTCTAAATTAGTACCACGTTAACTCCTTATCATGTTTAATAGCTGAGACTTAGTTTAACCCATTACATAATAAATCAACAACACACATTTAATGAATTGAAGTAAATGGACAAAATTGAGACTTAGTTTAACCTATTACATAAATAAGCCATTCCACACACATTGTTTTTCTATTTTTTAGGCCATATCCTCCATAGTTATTGGTAATTATTATACAGTAACTGTCCTAGCTCTGCCTCAGGCCCACATCTTTTGTTTCATTTGTTCTTGAACAATCACAAGTACTAAGATGAGAGTCGTCTATCTATTGCATTTCTTGTATTTAGCGTCTTTTAGTCTTTAGAGCTGTTGGTAGATTGACTTGCAAATGACATGGAAATATGATTTTTCTGGGAGAAGGATGGCTGAATGGATCAAAATGTTTTTATACATTACCATAACCATTTTTATGAAATAGTTTTCTCTGCATCTATCCATAGTTTAGTTGTGATTTTTTCCATGTAGCGGAAGAGAATTGGTATCAACATGGGTAAAAGAAAAGCTACTGTCAAGCATTCTAGGATCCAGCCAGAACTTCTGGACAAAGAAATGGAGTTTCCTAAAGTGAGCAGCAAGCAGGAGGTTGTAGCTAACACTGATTTCAACAATGACACAACAGGTTCTTTATGTTGCTCCTCTATGGTCGACCAAAGTGAAACATCTGATTGTAATGTCTTCTTTGGTGGAGACATTTCACCTGATTCAACTGGACTTCTCAGTGGTGCTGTTGAAGAACTATCTTCCCATGTTAAGTTTGATTGTTTGTTGAATAATGAGGAAGACCATGAATTAAATCTAGACAATGCTATGTTGGGAGATCATATGAAAGTTAGATCGAATTCAATTGGTTTAGATAAAGATCCTTCTCCAATAAAATGTATGATAGGTCTTGATGTCAGCCATACCAAGCTGGAGGAAGTTGAACCACCTATGGAGTCTGAAGGTTCATCCACAACTTCAGAAGATACTGAAGTTCAAAAGATTGACATTGATATTGGCATTGGACTGCCATTATTAGCTGAATCATCTTTTCTTCATATGGGAGCAGACTATAGCGAAAATGACCATGTTGTTGGATGTATTCATGAATCTGGAGAATGGGCAGTGTATTGGGATTCTTTTTATATGAGAAACTACTTCTATAATCTAAAGACTCATGAGTCTACTTGGAACCCTCCTCCAGGGTTGGAACATTTTGCAGTATCTGATGCCAATTGCACAGAAAATGAATCAATTGCTGAAGTTGCTGAAATGGATGTATTAGAAGATGTAAAATCAGAAGATATTTGTAGTGTGCTTGGAGATACACGGTCGTGTGTGAATTTACTTGGTGATAATGCTCATTGTCAACCACCTGATGGACTGTTGGAGGGCTCAGAAACTAGTGCATCTGTCCATACTTTAACCAACAGTCATAAGCATTCTGATGAACCTCATGAGTGGCAGACGAGTTGCAGAAATACCAGGGAAGATATCAGATGCAGGTTCTTAACTTCTTGCCTATGATTTCTATTTCCCCTTCTTTTTTACCTATATGAATAACTTTTTTGGAGAGGTATATTGATTACTTATTGTGATTTCAGTTGTTGCAGCAATGATTTTCAGTTCACTGTTGCAAATGGAGCTTTTGAACAAAAGACCTTCAGCCTTGGCAAACTTAGTAACGTGGACTTACCTGAGATAGATTGTATCACCAGAGATGATGGTGATGATGAAAGCACAATGGGTTTATATCCTAGCAGGTAGCATTTAGAATCAGAACTGTAATGGTGTCTATTAGTTTCTTTATATCACCACAAATAGCTCTTATGTTTGGGCTCCCTTGCTGTGTTTTCAGTGTTTCTCATATGCTTCAGCAGGCAGTTCATAGAGATGGTGATATGCATTTTGGAAATGGACCTACTATAGGTACACTGGTCACTGAGCAAAATCTTGCCGGAAGAAATAGAAAAAAGAAAATGAAAAGAACACGCCGACATAGACAGTTATTTGATAGAAATGAAGGTTCGTTATTTCTGCCTCCTCTACCATGCCTGCAAAATACTTAGGTTTATCATCAATAGACATTTTATTTACGTTATATACTCCATACAGTTTAATCAATTAGATCTTTTTGCCAGCATAAAGGAGGATATGGAAAGATTTTGGCAGCAGTAAATGGACAGATCAATTTGTTTGGTTGGTTAATGATTAAGAATATGACACATCTCCCTTTGACTTCACTTGTATTTCCCAATAGTTTTAACCCGCTTTTATGTGGTCCTATACATTGATTCTTATTCACAATTTATTTTTGTCATTCGAAGATTATAAGAACTTGACATACCAGTTAAAAATTCAGATTATCTCTTAATGTATGGGATCTTAATCTTAAATTAGTTACTGTTCTATATCTAGTCACTTTCAGTTCTATGCTATGCAGACAGGAAGAGGAGATTAGAGTTTGGAATAACCTTAAACATCAATAATCTGAAACAGAAATATGAACTTTGTCTTAGACTCTTAGTAAATGCTGATATATCTTAGAGGAAGCGAATAAATTCATGGGTAAATATTGTGTTTTCCCTTCCTCTTTGGACAAGTCTTCTTTATATGTCCTTATGCAAAATTAGTAGTTTGGTGTCTCCATTAAGATTATGAACCATATTTAGAGCTTTAGTATCACTCAACAATCTGAACGCGATGAATGTGGTAGAAGATCAATTTTGGCTGGTGGTTCCATCTGGTTGTAACTCATAGCATAGTTGCTACTGCCAAATTAGTCTCGGTGGGCTTGTTTTTTTGAATGCCTCATGATAGATAATGAAATGCCACAAGATTCTTTCCCCCCTGACAGAATAATTTCTTCTGCAGAATTTCAATCTCTCGCAATCACTGAAGAGTATCCCACAAGTATTGCTAAATATTGGTGTCAGAGGTATCAACTTTTCTCCAGATTTGATGATGGTGTAAAAATGGACAAAGAAGGATGGTTTTCTGTAACTCCAGAGCCAATAGCTAGACATCATGCATTACGTTGTAGTAGCAATATGATAATTGACTCTTTCACTGGGGTTGGTGGAAATGCCATCCAGTTTTCCCAAAGGTGATGTTCGGCTAATTTTATCTTTGTTCATTTTTCTAAGTTTATGGGGATAGTTGGCACTTGCATGTATCAGATAATTGGTTAGGATGTTGTATAGTTTATTTTTCCATGTATATCAGCGGTTGATGAATAAGTGGATCTGATCAGATTGATGTGTTATTCTCTTTTGCAACTTCTAAAATATATTTAATAGAATATTTCTGACAACATCTTTGTCTAACTAACTTGTTAATGATAGTACAGGGCCAAACATGTTATCGCAATTGATATTGATCCAATAAAAATTAGATATGCACAACATAATGCTGCCATTTATGGTGTTGAAGATCAAATAGATTTTGTGAAGGGGGATTTCTTCTGTTTGGCCCCAAGTCTCAAGGTATTGACCCTTCTTTTCTAGAAAAAGTATGAAGTTTTCTTTTACTGGAAATGAATGTTTGTTTAGAAAACCAATTTCCTGAGATGTGTAATGAAAGCTACCATATGTATGATTTTAAGTGTCTGTTATTTTGAAATTTTGAAGTTCTCCCTTTGACAGACTAAGAACTTGGATTGTCAATGTTTGTATTTTAGAAGTTGTGGTGAAAATATTACCTGTATGATCATTGTTTGGAGAAGGAATTACCATAAGAATGCTAATTATGCCTGTGTGAATGTCTTGAATACTATCATAAGAATATCAATTTTTTTTACTAGTAATATAATGGATGCAGTGTTTTGTTGCATCATTTATTCCTATTTTTCTGAAGGCAAGTCCTGCTGCAGCTGTCTTTTTAACTATGGTTTTGATCTTTCATATTCTATAAAAAAGTTTATTGATTTCTTGCAGTTTGGTTTTATTTAACTTTCAGTGCTATACTTCCATCTCACTGTTAATTATATTAAAATCTTGGTCTCTCCATTCTGCAGGCCGATGTTATATTCTTATCACCTCCTTGGGGAGGCCCTGATTATGCCAAAGTAGATATTTATGACCTTAAGACCATGCTCAAACCACATGATGGGTACGCTTTTGACTTTTATTGTTTGAGTTCAATTTGGTGTTGCACTAATGTTATTTTTATCATGTGACCTTTTCTTTTGCGTTTTGCCTGTAACTATGTAGGTATTTTCTCTTCAACATTGCTAAGAAAGTTGCTCCCCTTGTTGTCATGTTTCTTCCAAGGAATGTTAATTTTGACCAACTAGCCGAGCTGTCTCTTTCTTCAGATCCTCCGTGGTCACTTGAGGTAAGAATATACTTTTTTGTGCTCAGTATCTGTTCTTGTGAATTATTAGCAAGCTTTAGAATTGACATTAATAAATTGTAGATGTTATTGAAGCTGTTGTGGTGCCTTTTGTGGAATATGAAGAACAGGAGGTGTAGTATAATTGCTGAAATGAATATCTAGGTTTTGCTTTTCATTGTGCAATGTGCAATGTGCAGTCTCCAGTTAGAGCGGTTGTTTCTACTACAAGTTGAAATTGAGTTTTGCACCGTAAAGGAATGATTATGAGTCTTTACCTTGTCTCTCATATACTTGCAAACACGTGCAATTGAAAATGAATAATTCATGCATATGCATGCGAGCTTCCATACACTCTTAAATCATGAATGTGCTGACTAACACCTGCACTCCTCTCCTAATCTGTAGAAGGTTAACTCTTGTTTTGCTCGATGCAGTCTTCCTACTGATATAATCTATTACACCTTGACTGCGTTAAACACGATCCTTGTGAAAAATCCTTTTCCTATACATTGTATCTTATTCACTTTAAATTCTACTTACTTTTAATTGTGTTCTCAGGTTGAGAAAAACTTCTTAAATGGCAAGCTGAAGGCAATTACTGCTTACTTTAGCAATGGCTCTATGAACAAACCCATTGTCATCTGA

mRNA sequence

ATGGGATCTGGAAACGGAGAATCCGACGACGAAGTCGGAGCCTCTGCTATTAGGGCTCTCGGCTCTCTCTTCAAGTTGACCGAAGTCTTTCTCTGGGACGATGAGACAGAAACAGCTAGACGAGTGGAAAGCAGTTTAGCCCCTGATGCTGATGATACCAATAACGAAGAATTCGGGGAGAAAATCTGTTCTACAATCAGTGATATTAGCTTACAACCGGAGGATGTCGAACTTACTGAACAGATGAATGCTCTGGGGCTTCCTCTTTCGTTCCACACAAACAAAGAGCGGAAGAGAATTGGTATCAACATGGGTAAAAGAAAAGCTACTGTCAAGCATTCTAGGATCCAGCCAGAACTTCTGGACAAAGAAATGGAGTTTCCTAAAGTGAGCAGCAAGCAGGAGGTTGTAGCTAACACTGATTTCAACAATGACACAACAGGTTCTTTATGTTGCTCCTCTATGGTCGACCAAAGTGAAACATCTGATTGTAATGTCTTCTTTGGTGGAGACATTTCACCTGATTCAACTGGACTTCTCAGTGGTGCTGTTGAAGAACTATCTTCCCATGTTAAGTTTGATTGTTTGTTGAATAATGAGGAAGACCATGAATTAAATCTAGACAATGCTATGTTGGGAGATCATATGAAAGTTAGATCGAATTCAATTGGTTTAGATAAAGATCCTTCTCCAATAAAATGTATGATAGGTCTTGATGTCAGCCATACCAAGCTGGAGGAAGTTGAACCACCTATGGAGTCTGAAGGTTCATCCACAACTTCAGAAGATACTGAAGTTCAAAAGATTGACATTGATATTGGCATTGGACTGCCATTATTAGCTGAATCATCTTTTCTTCATATGGGAGCAGACTATAGCGAAAATGACCATGTTGTTGGATGTATTCATGAATCTGGAGAATGGGCAGTGTATTGGGATTCTTTTTATATGAGAAACTACTTCTATAATCTAAAGACTCATGAGTCTACTTGGAACCCTCCTCCAGGGTTGGAACATTTTGCAGTATCTGATGCCAATTGCACAGAAAATGAATCAATTGCTGAAGTTGCTGAAATGGATGTATTAGAAGATGTAAAATCAGAAGATATTTGTAGTGTGCTTGGAGATACACGGTCGTGTGTGAATTTACTTGGTGATAATGCTCATTGTCAACCACCTGATGGACTGTTGGAGGGCTCAGAAACTAGTGCATCTGTCCATACTTTAACCAACAGTCATAAGCATTCTGATGAACCTCATGAGTGGCAGACGAGTTGCAGAAATACCAGGGAAGATATCAGATGCAGCAATGATTTTCAGTTCACTGTTGCAAATGGAGCTTTTGAACAAAAGACCTTCAGCCTTGGCAAACTTAGTAACGTGGACTTACCTGAGATAGATTGTATCACCAGAGATGATGGTGATGATGAAAGCACAATGGGTTTATATCCTAGCAGTGTTTCTCATATGCTTCAGCAGGCAGTTCATAGAGATGGTGATATGCATTTTGGAAATGGACCTACTATAGGTACACTGGTCACTGAGCAAAATCTTGCCGGAAGAAATAGAAAAAAGAAAATGAAAAGAACACGCCGACATAGACAGTTATTTGATAGAAATGAAGAATTTCAATCTCTCGCAATCACTGAAGAGTATCCCACAAGTATTGCTAAATATTGGTGTCAGAGGTATCAACTTTTCTCCAGATTTGATGATGGTGTAAAAATGGACAAAGAAGGATGGTTTTCTGTAACTCCAGAGCCAATAGCTAGACATCATGCATTACGTTGTAGTAGCAATATGATAATTGACTCTTTCACTGGGGTTGGTGGAAATGCCATCCAGTTTTCCCAAAGGGCCAAACATGTTATCGCAATTGATATTGATCCAATAAAAATTAGATATGCACAACATAATGCTGCCATTTATGGTGTTGAAGATCAAATAGATTTTGTGAAGGGGGATTTCTTCTGTTTGGCCCCAAGTCTCAAGGCCGATGTTATATTCTTATCACCTCCTTGGGGAGGCCCTGATTATGCCAAAGTAGATATTTATGACCTTAAGACCATGCTCAAACCACATGATGGGTATTTTCTCTTCAACATTGCTAAGAAAGTTGCTCCCCTTGTTGTCATGTTTCTTCCAAGGAATGTTAATTTTGACCAACTAGCCGAGCTGTCTCTTTCTTCAGATCCTCCGTGGTCACTTGAGGTTGAGAAAAACTTCTTAAATGGCAAGCTGAAGGCAATTACTGCTTACTTTAGCAATGGCTCTATGAACAAACCCATTGTCATCTGA

Coding sequence (CDS)

ATGGGATCTGGAAACGGAGAATCCGACGACGAAGTCGGAGCCTCTGCTATTAGGGCTCTCGGCTCTCTCTTCAAGTTGACCGAAGTCTTTCTCTGGGACGATGAGACAGAAACAGCTAGACGAGTGGAAAGCAGTTTAGCCCCTGATGCTGATGATACCAATAACGAAGAATTCGGGGAGAAAATCTGTTCTACAATCAGTGATATTAGCTTACAACCGGAGGATGTCGAACTTACTGAACAGATGAATGCTCTGGGGCTTCCTCTTTCGTTCCACACAAACAAAGAGCGGAAGAGAATTGGTATCAACATGGGTAAAAGAAAAGCTACTGTCAAGCATTCTAGGATCCAGCCAGAACTTCTGGACAAAGAAATGGAGTTTCCTAAAGTGAGCAGCAAGCAGGAGGTTGTAGCTAACACTGATTTCAACAATGACACAACAGGTTCTTTATGTTGCTCCTCTATGGTCGACCAAAGTGAAACATCTGATTGTAATGTCTTCTTTGGTGGAGACATTTCACCTGATTCAACTGGACTTCTCAGTGGTGCTGTTGAAGAACTATCTTCCCATGTTAAGTTTGATTGTTTGTTGAATAATGAGGAAGACCATGAATTAAATCTAGACAATGCTATGTTGGGAGATCATATGAAAGTTAGATCGAATTCAATTGGTTTAGATAAAGATCCTTCTCCAATAAAATGTATGATAGGTCTTGATGTCAGCCATACCAAGCTGGAGGAAGTTGAACCACCTATGGAGTCTGAAGGTTCATCCACAACTTCAGAAGATACTGAAGTTCAAAAGATTGACATTGATATTGGCATTGGACTGCCATTATTAGCTGAATCATCTTTTCTTCATATGGGAGCAGACTATAGCGAAAATGACCATGTTGTTGGATGTATTCATGAATCTGGAGAATGGGCAGTGTATTGGGATTCTTTTTATATGAGAAACTACTTCTATAATCTAAAGACTCATGAGTCTACTTGGAACCCTCCTCCAGGGTTGGAACATTTTGCAGTATCTGATGCCAATTGCACAGAAAATGAATCAATTGCTGAAGTTGCTGAAATGGATGTATTAGAAGATGTAAAATCAGAAGATATTTGTAGTGTGCTTGGAGATACACGGTCGTGTGTGAATTTACTTGGTGATAATGCTCATTGTCAACCACCTGATGGACTGTTGGAGGGCTCAGAAACTAGTGCATCTGTCCATACTTTAACCAACAGTCATAAGCATTCTGATGAACCTCATGAGTGGCAGACGAGTTGCAGAAATACCAGGGAAGATATCAGATGCAGCAATGATTTTCAGTTCACTGTTGCAAATGGAGCTTTTGAACAAAAGACCTTCAGCCTTGGCAAACTTAGTAACGTGGACTTACCTGAGATAGATTGTATCACCAGAGATGATGGTGATGATGAAAGCACAATGGGTTTATATCCTAGCAGTGTTTCTCATATGCTTCAGCAGGCAGTTCATAGAGATGGTGATATGCATTTTGGAAATGGACCTACTATAGGTACACTGGTCACTGAGCAAAATCTTGCCGGAAGAAATAGAAAAAAGAAAATGAAAAGAACACGCCGACATAGACAGTTATTTGATAGAAATGAAGAATTTCAATCTCTCGCAATCACTGAAGAGTATCCCACAAGTATTGCTAAATATTGGTGTCAGAGGTATCAACTTTTCTCCAGATTTGATGATGGTGTAAAAATGGACAAAGAAGGATGGTTTTCTGTAACTCCAGAGCCAATAGCTAGACATCATGCATTACGTTGTAGTAGCAATATGATAATTGACTCTTTCACTGGGGTTGGTGGAAATGCCATCCAGTTTTCCCAAAGGGCCAAACATGTTATCGCAATTGATATTGATCCAATAAAAATTAGATATGCACAACATAATGCTGCCATTTATGGTGTTGAAGATCAAATAGATTTTGTGAAGGGGGATTTCTTCTGTTTGGCCCCAAGTCTCAAGGCCGATGTTATATTCTTATCACCTCCTTGGGGAGGCCCTGATTATGCCAAAGTAGATATTTATGACCTTAAGACCATGCTCAAACCACATGATGGGTATTTTCTCTTCAACATTGCTAAGAAAGTTGCTCCCCTTGTTGTCATGTTTCTTCCAAGGAATGTTAATTTTGACCAACTAGCCGAGCTGTCTCTTTCTTCAGATCCTCCGTGGTCACTTGAGGTTGAGAAAAACTTCTTAAATGGCAAGCTGAAGGCAATTACTGCTTACTTTAGCAATGGCTCTATGAACAAACCCATTGTCATCTGA

Protein sequence

MGSGNGESDDEVGASAIRALGSLFKLTEVFLWDDETETARRVESSLAPDADDTNNEEFGEKICSTISDISLQPEDVELTEQMNALGLPLSFHTNKERKRIGINMGKRKATVKHSRIQPELLDKEMEFPKVSSKQEVVANTDFNNDTTGSLCCSSMVDQSETSDCNVFFGGDISPDSTGLLSGAVEELSSHVKFDCLLNNEEDHELNLDNAMLGDHMKVRSNSIGLDKDPSPIKCMIGLDVSHTKLEEVEPPMESEGSSTTSEDTEVQKIDIDIGIGLPLLAESSFLHMGADYSENDHVVGCIHESGEWAVYWDSFYMRNYFYNLKTHESTWNPPPGLEHFAVSDANCTENESIAEVAEMDVLEDVKSEDICSVLGDTRSCVNLLGDNAHCQPPDGLLEGSETSASVHTLTNSHKHSDEPHEWQTSCRNTREDIRCSNDFQFTVANGAFEQKTFSLGKLSNVDLPEIDCITRDDGDDESTMGLYPSSVSHMLQQAVHRDGDMHFGNGPTIGTLVTEQNLAGRNRKKKMKRTRRHRQLFDRNEEFQSLAITEEYPTSIAKYWCQRYQLFSRFDDGVKMDKEGWFSVTPEPIARHHALRCSSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPIKIRYAQHNAAIYGVEDQIDFVKGDFFCLAPSLKADVIFLSPPWGGPDYAKVDIYDLKTMLKPHDGYFLFNIAKKVAPLVVMFLPRNVNFDQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYFSNGSMNKPIVI
Homology
BLAST of Lag0036955 vs. NCBI nr
Match: XP_022975951.1 (uncharacterized protein LOC111476503 isoform X1 [Cucurbita maxima] >XP_022975952.1 uncharacterized protein LOC111476503 isoform X1 [Cucurbita maxima] >XP_022975953.1 uncharacterized protein LOC111476503 isoform X1 [Cucurbita maxima] >XP_022975954.1 uncharacterized protein LOC111476503 isoform X1 [Cucurbita maxima] >XP_022975955.1 uncharacterized protein LOC111476503 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1176.4 bits (3042), Expect = 0.0e+00
Identity = 601/791 (75.98%), Postives = 652/791 (82.43%), Query Frame = 0

Query: 1   MGSGNGESDDEVGASAIRALGSLFKLTEVFLWDDETETARRVESSLAPDADDTNNEEFGE 60
           MGS N ES+DE G SAIRA+GSLFKLTEVFLWDDETE ARRVESSLA DADD NNE+F E
Sbjct: 1   MGSSNEESEDEAGVSAIRAIGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  KICSTISDISLQPEDVELTEQMNALGLPLSFHTNKERKRIGINMGKRKATVKHSRIQPEL 120
           KICSTI+DISL PED++LTEQMNALGLPLSFHTNKE++R GI MGKRK TVKHSRIQ   
Sbjct: 61  KICSTITDISLSPEDIQLTEQMNALGLPLSFHTNKEQRRTGITMGKRKTTVKHSRIQHGF 120

Query: 121 LDKEMEFPKVSSKQEVVANTDFNNDTTGSLCCSSMVDQSETSDC---------NVFFGGD 180
           LDKE+EFPK SS+ E+VAN + N++  GSLCCSSMV+QSE SDC         +V F GD
Sbjct: 121 LDKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180

Query: 181 ISPDSTGLLSGAVEELSSHVKFDCLLNNEEDHELNLDNAMLGDHMKVRSNSIGLDKDPSP 240
           ISP+S+GL+ GAVEE S +VK D +LNN  DHE    +A+LGDH KVR + IGLDK  SP
Sbjct: 181 ISPNSSGLIHGAVEEQSCNVKCDIVLNNRGDHESG--DALLGDHAKVRLSPIGLDKGHSP 240

Query: 241 IKCMIGLDVSHTKLEEVEPPMESEGSSTTSEDTEVQKIDIDIGIGLPLLAESSFLHMGAD 300
             CM G DVSH K EEVE PME EGSSTT +DTEVQKIDID GIGLPL+AE S+LHMGAD
Sbjct: 241 RICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSYLHMGAD 300

Query: 301 YSENDHVVGCIHESGEWAVYWDSFYMRNYFYNLKTHESTWNPPPGLEHFAVSDANCTENE 360
           Y+ENDHVVGCI E GEW VYWDSFYMRNYFYN+KTHESTWNPPPGLEHFA SDAN TENE
Sbjct: 301 YNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHSDANFTENE 360

Query: 361 SIAEVAEMDVLEDVKSEDICSVLGDTRSCVNLLGDNAHCQPPDGLL-------EGSETSA 420
           SIAEVAEMDVLED K EDICSVL DTRSC+NL GDN HCQPPD LL       EGS++ A
Sbjct: 361 SIAEVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKSRA 420

Query: 421 SVHTLTNSHKHSDEPHEWQTSCRNTREDI--------------RCSNDFQFTVANGAFEQ 480
           SVHT  NS+   DEPHEW TSCRNTRE I               CSN FQ  VAN   EQ
Sbjct: 421 SVHTSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANETSEQ 480

Query: 481 KTFSLGKLSNVDLPEIDCITRDDGDDESTMGLYPSSVSHMLQQAVHRDGDMHFGNGPTIG 540
           KTFS  K SN+D PE   +T    DDE  +GL  SSVSH+LQQA H DGDMHFGN PTI 
Sbjct: 481 KTFSHCKSSNMDSPEKAFVTI---DDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTIC 540

Query: 541 TLVTEQNLAGRNRKKKMKRTRRHRQLFDRNEEFQSLAITEEYPTSIAKYWCQRYQLFSRF 600
           TL TEQNL+GR+RKKKMKRTRR  QL DRNEEF SLAITEEYPTSI KYWCQRYQLFSRF
Sbjct: 541 TLGTEQNLSGRDRKKKMKRTRRRGQLSDRNEEFHSLAITEEYPTSITKYWCQRYQLFSRF 600

Query: 601 DDGVKMDKEGWFSVTPEPIARHHALRCSSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
           DDGVKMDKEGWFSVTPE IARHHA RC SNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP
Sbjct: 601 DDGVKMDKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660

Query: 661 IKIRYAQHNAAIYGVEDQIDFVKGDFFCLAPSLKADVIFLSPPWGGPDYAKVDIYDLKTM 720
           IKIRYAQHNAA+YGVEDQIDF+KGDFF LAP LKADVIFLSPPWGGP+YA+VDIYDLKTM
Sbjct: 661 IKIRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTM 720

Query: 721 LKPHDGYFLFNIAKKVAPLVVMFLPRNVNFDQLAELSLSSDPPWSLEVEKNFLNGKLKAI 762
           L+PHDGYFLFNIAKK+AP+VVMFLP+NVN DQLAE++LSS+PPWSLEVEKNFLNGKLKAI
Sbjct: 721 LRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAI 780

BLAST of Lag0036955 vs. NCBI nr
Match: XP_022975956.1 (uncharacterized protein LOC111476503 isoform X2 [Cucurbita maxima])

HSP 1 Score: 1172.9 bits (3033), Expect = 0.0e+00
Identity = 602/791 (76.11%), Postives = 651/791 (82.30%), Query Frame = 0

Query: 1   MGSGNGESDDEVGASAIRALGSLFKLTEVFLWDDETETARRVESSLAPDADDTNNEEFGE 60
           MGS N ES+DE G SAIRA+GSLFKLTEVFLWDDETE ARRVESSLA DADD NNE+F E
Sbjct: 1   MGSSNEESEDEAGVSAIRAIGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  KICSTISDISLQPEDVELTEQMNALGLPLSFHTNKERKRIGINMGKRKATVKHSRIQPEL 120
           KICSTI+DISL PED++LTEQMNALGLPLSFHTNKER R GI MGKRK TVKHSRIQ   
Sbjct: 61  KICSTITDISLSPEDIQLTEQMNALGLPLSFHTNKER-RTGITMGKRKTTVKHSRIQHGF 120

Query: 121 LDKEMEFPKVSSKQEVVANTDFNNDTTGSLCCSSMVDQSETSDC---------NVFFGGD 180
           LDKE+EFPK SS+ E+VAN + N++  GSLCCSSMV+QSE SDC         +V F GD
Sbjct: 121 LDKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180

Query: 181 ISPDSTGLLSGAVEELSSHVKFDCLLNNEEDHELNLDNAMLGDHMKVRSNSIGLDKDPSP 240
           ISP+S+GL+ GAVEE S +VK D +LNN  DHE    +A+LGDH KVR + IGLDK  SP
Sbjct: 181 ISPNSSGLIHGAVEEQSCNVKCDIVLNNRGDHESG--DALLGDHAKVRLSPIGLDKGHSP 240

Query: 241 IKCMIGLDVSHTKLEEVEPPMESEGSSTTSEDTEVQKIDIDIGIGLPLLAESSFLHMGAD 300
             CM G DVSH K EEVE PME EGSSTT +DTEVQKIDID GIGLPL+AE S+LHMGAD
Sbjct: 241 RICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSYLHMGAD 300

Query: 301 YSENDHVVGCIHESGEWAVYWDSFYMRNYFYNLKTHESTWNPPPGLEHFAVSDANCTENE 360
           Y+ENDHVVGCI E GEW VYWDSFYMRNYFYN+KTHESTWNPPPGLEHFA SDAN TENE
Sbjct: 301 YNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHSDANFTENE 360

Query: 361 SIAEVAEMDVLEDVKSEDICSVLGDTRSCVNLLGDNAHCQPPDGLL-------EGSETSA 420
           SIAEVAEMDVLED K EDICSVL DTRSC+NL GDN HCQPPD LL       EGS++ A
Sbjct: 361 SIAEVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKSRA 420

Query: 421 SVHTLTNSHKHSDEPHEWQTSCRNTREDI--------------RCSNDFQFTVANGAFEQ 480
           SVHT  NS+   DEPHEW TSCRNTRE I               CSN FQ  VAN   EQ
Sbjct: 421 SVHTSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANETSEQ 480

Query: 481 KTFSLGKLSNVDLPEIDCITRDDGDDESTMGLYPSSVSHMLQQAVHRDGDMHFGNGPTIG 540
           KTFS  K SN+D PE   +T    DDE  +GL  SSVSH+LQQA H DGDMHFGN PTI 
Sbjct: 481 KTFSHCKSSNMDSPEKAFVTI---DDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTIC 540

Query: 541 TLVTEQNLAGRNRKKKMKRTRRHRQLFDRNEEFQSLAITEEYPTSIAKYWCQRYQLFSRF 600
           TL TEQNL+GR+RKKKMKRTRR  QL DRNEEF SLAITEEYPTSI KYWCQRYQLFSRF
Sbjct: 541 TLGTEQNLSGRDRKKKMKRTRRRGQLSDRNEEFHSLAITEEYPTSITKYWCQRYQLFSRF 600

Query: 601 DDGVKMDKEGWFSVTPEPIARHHALRCSSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
           DDGVKMDKEGWFSVTPE IARHHA RC SNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP
Sbjct: 601 DDGVKMDKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660

Query: 661 IKIRYAQHNAAIYGVEDQIDFVKGDFFCLAPSLKADVIFLSPPWGGPDYAKVDIYDLKTM 720
           IKIRYAQHNAA+YGVEDQIDF+KGDFF LAP LKADVIFLSPPWGGP+YA+VDIYDLKTM
Sbjct: 661 IKIRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTM 720

Query: 721 LKPHDGYFLFNIAKKVAPLVVMFLPRNVNFDQLAELSLSSDPPWSLEVEKNFLNGKLKAI 762
           L+PHDGYFLFNIAKK+AP+VVMFLP+NVN DQLAE++LSS+PPWSLEVEKNFLNGKLKAI
Sbjct: 721 LRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAI 780

BLAST of Lag0036955 vs. NCBI nr
Match: XP_023535334.1 (uncharacterized protein LOC111796805 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023535335.1 uncharacterized protein LOC111796805 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023535336.1 uncharacterized protein LOC111796805 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1163.7 bits (3009), Expect = 0.0e+00
Identity = 598/791 (75.60%), Postives = 648/791 (81.92%), Query Frame = 0

Query: 1   MGSGNGESDDEVGASAIRALGSLFKLTEVFLWDDETETARRVESSLAPDADDTNNEEFGE 60
           MGS N ES+DE G SAIRALGSLFKLTEVFLWDDETE ARRVESSLA DADD NNE+F E
Sbjct: 1   MGSSNEESEDEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  KICSTISDISLQPEDVELTEQMNALGLPLSFHTNKERKRIGINMGKRKATVKHSRIQPEL 120
           KICSTI+DISL PED+ELTEQMNALGLPLSFHTNKE++R GI MGKRK TVKHSRIQ   
Sbjct: 61  KICSTITDISLSPEDIELTEQMNALGLPLSFHTNKEQRRTGITMGKRKTTVKHSRIQQGF 120

Query: 121 LDKEMEFPKVSSKQEVVANTDFNNDTTGSLCCSSMVDQSETSDC---------NVFFGGD 180
           LDKE+EFPK SS+ E+VAN + N++  GSLCCSSMV+QSE SDC         +V F GD
Sbjct: 121 LDKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180

Query: 181 ISPDSTGLLSGAVEELSSHVKFDCLLNNEEDHELNLDNAMLGDHMKVRSNSIGLDKDPSP 240
           ISP+S+GL+ GAVEE    V  D +LNN  DHE    +A+LGDH KVR +SIGLDK  SP
Sbjct: 181 ISPNSSGLIHGAVEEQFCDVTCDIVLNNRGDHESG--DAVLGDHAKVRLSSIGLDKGHSP 240

Query: 241 IKCMIGLDVSHTKLEEVEPPMESEGSSTTSEDTEVQKIDIDIGIGLPLLAESSFLHMGAD 300
             CM G DVSH K EEVE PME EGSSTT +DTEVQKIDID GIGLPL+AE SFLHMGAD
Sbjct: 241 RICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMGAD 300

Query: 301 YSENDHVVGCIHESGEWAVYWDSFYMRNYFYNLKTHESTWNPPPGLEHFAVSDANCTENE 360
           Y+ENDHVVGCI E GEW VYWDSFYMRNYFYN+KTHESTWNPPPGLEHFA  DAN TENE
Sbjct: 301 YNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHFDANFTENE 360

Query: 361 SIAEVAEMDVLEDVKSEDICSVLGDTRSCVNLLGDNAHCQPPDGLL-------EGSETSA 420
           SIAEVAEMDVLED K EDICSVL DTRSC+NL GDN HCQPPD LL       EGS++ A
Sbjct: 361 SIAEVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLESSGILVEGSKSRA 420

Query: 421 SVHTLTNSHKHSDEPHEWQTSCRNTREDI--------------RCSNDFQFTVANGAFEQ 480
           SV+T  +S+   DEPHEW TSCRNTRE I               CSN FQ  VAN A EQ
Sbjct: 421 SVNTSIHSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQ 480

Query: 481 KTFSLGKLSNVDLPEIDCITRDDGDDESTMGLYPSSVSHMLQQAVHRDGDMHFGNGPTIG 540
           KTFS  K SN+  PE   +T    DDE  +GL  SSVSH+LQQA H DGDMHFGN PTI 
Sbjct: 481 KTFSHCKPSNMYAPEKAFVTI---DDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTIC 540

Query: 541 TLVTEQNLAGRNRKKKMKRTRRHRQLFDRNEEFQSLAITEEYPTSIAKYWCQRYQLFSRF 600
           TL TEQNL+GR+RKKKMKRTRR  QL D+NEEF S AITEEYPTSI KYWCQRYQLFSRF
Sbjct: 541 TLGTEQNLSGRDRKKKMKRTRRRGQLSDKNEEFHSPAITEEYPTSITKYWCQRYQLFSRF 600

Query: 601 DDGVKMDKEGWFSVTPEPIARHHALRCSSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
           DDGVKMDKEGWFSVTPE IARHHA RC SNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP
Sbjct: 601 DDGVKMDKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660

Query: 661 IKIRYAQHNAAIYGVEDQIDFVKGDFFCLAPSLKADVIFLSPPWGGPDYAKVDIYDLKTM 720
            KIRYAQHNAA+YGVEDQIDF+KGDFF LAP LKADVIFLSPPWGGP+YA+VDIYDLKTM
Sbjct: 661 TKIRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTM 720

Query: 721 LKPHDGYFLFNIAKKVAPLVVMFLPRNVNFDQLAELSLSSDPPWSLEVEKNFLNGKLKAI 762
           L+PHDGYFLFNIAKK+AP+VVMFLP+NVN DQLAE++LSS+PPWSLEVEKNFLNGKLKAI
Sbjct: 721 LRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAI 780

BLAST of Lag0036955 vs. NCBI nr
Match: XP_022138324.1 (uncharacterized protein LOC111009535 isoform X1 [Momordica charantia] >XP_022138331.1 uncharacterized protein LOC111009535 isoform X1 [Momordica charantia] >XP_022138341.1 uncharacterized protein LOC111009535 isoform X1 [Momordica charantia])

HSP 1 Score: 1160.6 bits (3001), Expect = 0.0e+00
Identity = 589/777 (75.80%), Postives = 647/777 (83.27%), Query Frame = 0

Query: 1   MGSGNGESDDEVGASAIRALGSLFKLTEVFLWDDETETARRVESSLAPDADDTNNEEFGE 60
           MG  NGES+DEVGA AIRALG LFKLTEVFLWDDETE ARR E SLAPDADDT+NE+ GE
Sbjct: 1   MGFDNGESEDEVGAPAIRALGPLFKLTEVFLWDDETEAARRAERSLAPDADDTHNEDLGE 60

Query: 61  KICSTISDISLQPEDVELTEQMNALGLPLSFHTNKERKRIGINMGKRKATVKHSRIQPEL 120
           K+CSTIS ISL PED ELT+QMNALGLPLSFHTNKE KR GI  GKR+AT+KHSRIQ  L
Sbjct: 61  KVCSTISGISLLPEDTELTQQMNALGLPLSFHTNKE-KRNGITTGKRRATIKHSRIQQGL 120

Query: 121 LDKEMEFPKVSSKQEVVANTDFNNDTTGSLCCSSMVDQSETSDCNVFFGGDISPDSTGLL 180
           LDKEMEFP+VSS +E+VAN +FN+D  GSLCC+SMVDQSE SDC+VF  GDI P+STGL+
Sbjct: 121 LDKEMEFPRVSSGEEIVANLNFNDDAIGSLCCASMVDQSEASDCHVFLDGDILPNSTGLI 180

Query: 181 SGAVEELSSHVKFDCLLNNEEDHELNLDNAMLGDHMKVRSNSIGLDKDPSPIKCMIGLDV 240
           S  VEE S ++  D LLNN EDHE +  NA+LG+H  V S+SI LDK  SP + MIG+DV
Sbjct: 181 SSTVEEQSHNLMIDSLLNNGEDHESSPGNAVLGNHSIVTSSSISLDKVHSPKRYMIGIDV 240

Query: 241 SHTKLEEVEPPMESEGSSTTSEDTEVQKIDIDIGIGLPLLAESSFLHMGADYSENDHVVG 300
           SH+  E +EPPME EGSS T +DTEVQKI+ID GIGLPL+AESS LHM A Y ENDHVVG
Sbjct: 241 SHS--EALEPPMELEGSSMTLQDTEVQKINIDSGIGLPLVAESSLLHMEAGYDENDHVVG 300

Query: 301 CIHESGEWAVYWDSFYMRNYFYNLKTHESTWNPPPGLEHFAVSDANCTENESIAEVAEMD 360
           CIHE GEW VYWDSFYMRNYFYN+K+HESTWNPP GLEHFA+SDA+CT NESIAEVAEMD
Sbjct: 301 CIHEFGEWMVYWDSFYMRNYFYNIKSHESTWNPPAGLEHFALSDADCTANESIAEVAEMD 360

Query: 361 VLEDVKSEDICSVLGDTRSCVNLLGDNAHCQPPDGLLEGSETSASVHTLTNSHKHSDEPH 420
           V+EDVKS+DICSVLG+T S           +    L+E  ETS  ++T TNSHKHSD+PH
Sbjct: 361 VVEDVKSQDICSVLGETLS-----------EGSSILIESPETSGFINTSTNSHKHSDDPH 420

Query: 421 EWQTSCRNTREDI--------------RCSNDFQFTVANGAFEQKTFSLGKLSNVDLPEI 480
           + QTSCRNTR+DI               CSNDFQ T ANGA EQKTFSLGK SN+D PEI
Sbjct: 421 DCQTSCRNTRKDIGCSCEGHVKQLCREHCSNDFQLTFANGALEQKTFSLGKPSNIDSPEI 480

Query: 481 DCITRDD-GDDESTMGLYPSSVSHMLQQAVHRDGDMHFGNGPTIGTLVTEQNLAGRNRKK 540
           D +TRDD  DDE TM +Y SSVSHM+QQA  RD  M FGNG TI TL  E+NLAG +RKK
Sbjct: 481 DIVTRDDYDDDEGTMSIYTSSVSHMIQQADQRDSGMDFGNGRTICTLGAEKNLAGISRKK 540

Query: 541 KMKRTRRHRQLFDRNEEFQSLAITEEYPTSIAKYWCQRYQLFSRFDDGVKMDKEGWFSVT 600
           KMKR RR RQL +RNEEFQSLAITEEYPTSIAKYWCQRYQLFSRFDDGVKMD+EGWFSVT
Sbjct: 541 KMKRIRRRRQLSERNEEFQSLAITEEYPTSIAKYWCQRYQLFSRFDDGVKMDEEGWFSVT 600

Query: 601 PEPIARHHALRCSSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPIKIRYAQHNAAIYGV 660
           PEPIA+HHALRC  N+I+DSFTGVGGNAIQFSQRAKHVIAIDIDP KIRYA HNA+IYGV
Sbjct: 601 PEPIAQHHALRCGCNLIVDSFTGVGGNAIQFSQRAKHVIAIDIDPRKIRYAHHNASIYGV 660

Query: 661 EDQIDFVKGDFFCLAPSLKADVIFLSPPWGGPDYAKVDIYDLKTMLKPHDGYFLFNIAKK 720
           EDQIDF+KGDFF LAPSLKADVIFLSPPWGGPDY KVD YDLKTMLKPHDGYFLFNIAKK
Sbjct: 661 EDQIDFIKGDFFRLAPSLKADVIFLSPPWGGPDYTKVDTYDLKTMLKPHDGYFLFNIAKK 720

Query: 721 VAPLVVMFLPRNVNFDQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYFSNGSMNKP 763
           +AP VVMFLPRNVN DQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYFSNGS N+P
Sbjct: 721 IAPFVVMFLPRNVNVDQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYFSNGSTNEP 763

BLAST of Lag0036955 vs. NCBI nr
Match: XP_023535337.1 (uncharacterized protein LOC111796805 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1160.2 bits (3000), Expect = 0.0e+00
Identity = 599/791 (75.73%), Postives = 647/791 (81.80%), Query Frame = 0

Query: 1   MGSGNGESDDEVGASAIRALGSLFKLTEVFLWDDETETARRVESSLAPDADDTNNEEFGE 60
           MGS N ES+DE G SAIRALGSLFKLTEVFLWDDETE ARRVESSLA DADD NNE+F E
Sbjct: 1   MGSSNEESEDEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  KICSTISDISLQPEDVELTEQMNALGLPLSFHTNKERKRIGINMGKRKATVKHSRIQPEL 120
           KICSTI+DISL PED+ELTEQMNALGLPLSFHTNKER R GI MGKRK TVKHSRIQ   
Sbjct: 61  KICSTITDISLSPEDIELTEQMNALGLPLSFHTNKER-RTGITMGKRKTTVKHSRIQQGF 120

Query: 121 LDKEMEFPKVSSKQEVVANTDFNNDTTGSLCCSSMVDQSETSDC---------NVFFGGD 180
           LDKE+EFPK SS+ E+VAN + N++  GSLCCSSMV+QSE SDC         +V F GD
Sbjct: 121 LDKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180

Query: 181 ISPDSTGLLSGAVEELSSHVKFDCLLNNEEDHELNLDNAMLGDHMKVRSNSIGLDKDPSP 240
           ISP+S+GL+ GAVEE    V  D +LNN  DHE    +A+LGDH KVR +SIGLDK  SP
Sbjct: 181 ISPNSSGLIHGAVEEQFCDVTCDIVLNNRGDHESG--DAVLGDHAKVRLSSIGLDKGHSP 240

Query: 241 IKCMIGLDVSHTKLEEVEPPMESEGSSTTSEDTEVQKIDIDIGIGLPLLAESSFLHMGAD 300
             CM G DVSH K EEVE PME EGSSTT +DTEVQKIDID GIGLPL+AE SFLHMGAD
Sbjct: 241 RICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMGAD 300

Query: 301 YSENDHVVGCIHESGEWAVYWDSFYMRNYFYNLKTHESTWNPPPGLEHFAVSDANCTENE 360
           Y+ENDHVVGCI E GEW VYWDSFYMRNYFYN+KTHESTWNPPPGLEHFA  DAN TENE
Sbjct: 301 YNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHFDANFTENE 360

Query: 361 SIAEVAEMDVLEDVKSEDICSVLGDTRSCVNLLGDNAHCQPPDGLL-------EGSETSA 420
           SIAEVAEMDVLED K EDICSVL DTRSC+NL GDN HCQPPD LL       EGS++ A
Sbjct: 361 SIAEVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLESSGILVEGSKSRA 420

Query: 421 SVHTLTNSHKHSDEPHEWQTSCRNTREDI--------------RCSNDFQFTVANGAFEQ 480
           SV+T  +S+   DEPHEW TSCRNTRE I               CSN FQ  VAN A EQ
Sbjct: 421 SVNTSIHSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQ 480

Query: 481 KTFSLGKLSNVDLPEIDCITRDDGDDESTMGLYPSSVSHMLQQAVHRDGDMHFGNGPTIG 540
           KTFS  K SN+  PE   +T    DDE  +GL  SSVSH+LQQA H DGDMHFGN PTI 
Sbjct: 481 KTFSHCKPSNMYAPEKAFVTI---DDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTIC 540

Query: 541 TLVTEQNLAGRNRKKKMKRTRRHRQLFDRNEEFQSLAITEEYPTSIAKYWCQRYQLFSRF 600
           TL TEQNL+GR+RKKKMKRTRR  QL D+NEEF S AITEEYPTSI KYWCQRYQLFSRF
Sbjct: 541 TLGTEQNLSGRDRKKKMKRTRRRGQLSDKNEEFHSPAITEEYPTSITKYWCQRYQLFSRF 600

Query: 601 DDGVKMDKEGWFSVTPEPIARHHALRCSSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
           DDGVKMDKEGWFSVTPE IARHHA RC SNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP
Sbjct: 601 DDGVKMDKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660

Query: 661 IKIRYAQHNAAIYGVEDQIDFVKGDFFCLAPSLKADVIFLSPPWGGPDYAKVDIYDLKTM 720
            KIRYAQHNAA+YGVEDQIDF+KGDFF LAP LKADVIFLSPPWGGP+YA+VDIYDLKTM
Sbjct: 661 TKIRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTM 720

Query: 721 LKPHDGYFLFNIAKKVAPLVVMFLPRNVNFDQLAELSLSSDPPWSLEVEKNFLNGKLKAI 762
           L+PHDGYFLFNIAKK+AP+VVMFLP+NVN DQLAE++LSS+PPWSLEVEKNFLNGKLKAI
Sbjct: 721 LRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAI 780

BLAST of Lag0036955 vs. ExPASy Swiss-Prot
Match: P85107 (Trimethylguanosine synthase OS=Rattus norvegicus OX=10116 GN=Tgs1 PE=1 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 3.2e-67
Identity = 126/238 (52.94%), Postives = 170/238 (71.43%), Query Frame = 0

Query: 523 RKKKMKRTRRHRQLFDRNEEFQSLAITEEYPTSIAKYWCQRYQLFSRFDDGVKMDKEGWF 582
           +KKK K+  +++++     E  S+         +AKYW QRY+LFSRFDDG+K+DKEGWF
Sbjct: 612 KKKKKKKKNKNKKINGLPPEIASV-------PELAKYWAQRYRLFSRFDDGIKLDKEGWF 671

Query: 583 SVTPEPIARHHALRCSS----NMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPIKIRYAQH 642
           SVTPE IA H A R S     ++I+D+F GVGGN IQF+   K VIAIDIDP+KI  A++
Sbjct: 672 SVTPEKIAEHIAGRVSQSFNCDIIVDAFCGVGGNTIQFALTGKRVIAIDIDPVKIDLARN 731

Query: 643 NAAIYGVEDQIDFVKGDFFCLAPSLKADVIFLSPPWGGPDYAKVDIYDLKTMLKPHDGYF 702
           NA +YGV D+I+F+ GDF  LAP LKADV+FLSPPWGGPDYA  + +D++TM+ P DG+ 
Sbjct: 732 NAEVYGVADKIEFICGDFLLLAPCLKADVVFLSPPWGGPDYATAETFDIRTMMSP-DGFE 791

Query: 703 LFNIAKKVAPLVVMFLPRNVNFDQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYFSN 757
           +F +++K+   +V FLPRN + DQ+A L   + P   +E+E+NFLN KLK ITAYF +
Sbjct: 792 IFRLSQKITNNIVYFLPRNADVDQVASL---AGPGGQVEIEQNFLNNKLKTITAYFGD 838

BLAST of Lag0036955 vs. ExPASy Swiss-Prot
Match: Q923W1 (Trimethylguanosine synthase OS=Mus musculus OX=10090 GN=Tgs1 PE=1 SV=2)

HSP 1 Score: 254.6 bits (649), Expect = 3.5e-66
Identity = 123/236 (52.12%), Postives = 168/236 (71.19%), Query Frame = 0

Query: 525 KKMKRTRRHRQLFDRNEEFQSLAITEEYPTSIAKYWCQRYQLFSRFDDGVKMDKEGWFSV 584
           KK K+  +++++ D   E  S+         +AKYW QRY+LFSRFDDG+K+DKEGWFSV
Sbjct: 612 KKKKKKNKNKKINDLPPEIASV-------PELAKYWAQRYRLFSRFDDGIKLDKEGWFSV 671

Query: 585 TPEPIARHHALRCSS----NMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPIKIRYAQHNA 644
           TPE IA H A R S     ++++D+F GVGGN IQF+   K VIAIDIDP+KI  A++NA
Sbjct: 672 TPEKIAEHIAGRVSQAFRCDVVVDAFCGVGGNTIQFALTGKRVIAIDIDPVKIDLARNNA 731

Query: 645 AIYGVEDQIDFVKGDFFCLAPSLKADVIFLSPPWGGPDYAKVDIYDLKTMLKPHDGYFLF 704
            +YG+ D+I+F+ GDF  LAP LKADV+FLSPPWGGPDYA  + +D++TM+ P DG+ +F
Sbjct: 732 EVYGIADKIEFICGDFLLLAPCLKADVVFLSPPWGGPDYATAETFDIRTMMSP-DGFEIF 791

Query: 705 NIAKKVAPLVVMFLPRNVNFDQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYFSN 757
            +++K+   +V FLPRN + DQ+A L+        +E+E+NFLN KLK ITAYF +
Sbjct: 792 RLSQKITNNIVYFLPRNADIDQVASLAGLGG---QVEIEQNFLNNKLKTITAYFGD 836

BLAST of Lag0036955 vs. ExPASy Swiss-Prot
Match: Q96RS0 (Trimethylguanosine synthase OS=Homo sapiens OX=9606 GN=TGS1 PE=1 SV=3)

HSP 1 Score: 250.4 bits (638), Expect = 6.7e-65
Identity = 122/238 (51.26%), Postives = 165/238 (69.33%), Query Frame = 0

Query: 523 RKKKMKRTRRHRQLFDRNEEFQSLAITEEYPTSIAKYWCQRYQLFSRFDDGVKMDKEGWF 582
           +KKK K+         +N++   L         +AKYW QRY+LFSRFDDG+K+D+EGWF
Sbjct: 620 KKKKNKK---------KNKKVNGLPPEIAAVPELAKYWAQRYRLFSRFDDGIKLDREGWF 679

Query: 583 SVTPEPIARHHALRCSS----NMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPIKIRYAQH 642
           SVTPE IA H A R S     ++++D+F GVGGN IQF+     VIAIDIDP+KI  A++
Sbjct: 680 SVTPEKIAEHIAGRVSQSFKCDVVVDAFCGVGGNTIQFALTGMRVIAIDIDPVKIALARN 739

Query: 643 NAAIYGVEDQIDFVKGDFFCLAPSLKADVIFLSPPWGGPDYAKVDIYDLKTMLKPHDGYF 702
           NA +YG+ D+I+F+ GDF  LA  LKADV+FLSPPWGGPDYA  + +D++TM+ P DG+ 
Sbjct: 740 NAEVYGIADKIEFICGDFLLLASFLKADVVFLSPPWGGPDYATAETFDIRTMMSP-DGFE 799

Query: 703 LFNIAKKVAPLVVMFLPRNVNFDQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYFSN 757
           +F ++KK+   +V FLPRN + DQ+A L   + P   +E+E+NFLN KLK ITAYF +
Sbjct: 800 IFRLSKKITNNIVYFLPRNADIDQVASL---AGPGGQVEIEQNFLNNKLKTITAYFGD 844

BLAST of Lag0036955 vs. ExPASy Swiss-Prot
Match: Q09814 (Trimethylguanosine synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tgs1 PE=1 SV=3)

HSP 1 Score: 161.8 bits (408), Expect = 3.1e-38
Identity = 91/229 (39.74%), Postives = 129/229 (56.33%), Query Frame = 0

Query: 538 DRNEEFQSLAITEEYPTSIAKYWCQRYQLFSRFDDGVKMDKEGWFSVTPEPIARHHALR- 597
           D +E  +   I    P ++ KYW  RY LFSRFD+G+ +D + W+SVTPE +A   A   
Sbjct: 8   DEDELLKKCIICPPVPKALKKYWNNRYNLFSRFDEGIWLDYQSWYSVTPEKVAVAIAKSV 67

Query: 598 ---CSSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPIKIRYAQHNAAIYGV-EDQIDFV 657
                  +IID+F+G GGN IQF++    VI+I+IDPIKI  A+HN  IYG+   ++ F+
Sbjct: 68  VDFIQPELIIDAFSGCGGNTIQFAKYCP-VISIEIDPIKIAMAKHNLEIYGIPSSRVTFI 127

Query: 658 KGDFFCLAPSLK-----ADVIFLSPPWGGPDYAKVDIYDLKTMLKPHDGYFLFNIAKKVA 717
           +GD      SL+       ++F+SPPWGGP Y+   +Y L   L P+    LF  A +++
Sbjct: 128 QGDVLDTFKSLQFAKDYRSLVFMSPPWGGPSYSGKTVYSLND-LNPYAFDVLFKEATRIS 187

Query: 718 PLVVMFLPRNVNFDQLAELSLSSDPPWSLEVEKNFL-NGKLKAITAYFS 756
           P V  FLPRN +  +LA      + P+      NFL  G  KAI  YF+
Sbjct: 188 PYVAAFLPRNTDVKELAAYGSIHNKPYI----TNFLFEGYAKAICCYFN 230

BLAST of Lag0036955 vs. ExPASy Swiss-Prot
Match: Q12052 (Trimethylguanosine synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TGS1 PE=1 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 1.9e-27
Identity = 72/178 (40.45%), Postives = 98/178 (55.06%), Query Frame = 0

Query: 558 KYWCQRYQLFSRFDD-GVKMDKEGWFSVTPEPIA---RHHALRCSSN--MIIDSFTGVGG 617
           KYW  R +LFS+ D   + M  E WFSVTPE IA    +    C  N   I+D F G GG
Sbjct: 51  KYWKNRRRLFSKIDSASIYMTDELWFSVTPERIACFLANFVKACMPNAERILDVFCGGGG 110

Query: 618 NAIQFSQRAKHVIAIDIDPIKIRYAQHNAAIYGVEDQIDFVKGDFFCLA-----PSLKAD 677
           N IQF+ +  +V  +D     I     NA  YGV+D+I   +G +  L        +K D
Sbjct: 111 NTIQFAMQFPYVYGVDYSIEHIYCTAKNAQSYGVDDRIWLKRGSWKKLVSKQKLSKIKYD 170

Query: 678 VIFLSPPWGGPDYAKVDIYDLKTMLKPHDGYFLFNIAKKVAPLVVMFLPRNVNFDQLA 725
            +F SPPWGGP+Y + D+YDL+  LKP     +     K++P V+MFLPRN + +QL+
Sbjct: 171 CVFGSPPWGGPEYLRNDVYDLEQHLKPMGITKMLKSFLKLSPNVIMFLPRNSDLNQLS 228

BLAST of Lag0036955 vs. ExPASy TrEMBL
Match: A0A6J1II65 (Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=1)

HSP 1 Score: 1176.4 bits (3042), Expect = 0.0e+00
Identity = 601/791 (75.98%), Postives = 652/791 (82.43%), Query Frame = 0

Query: 1   MGSGNGESDDEVGASAIRALGSLFKLTEVFLWDDETETARRVESSLAPDADDTNNEEFGE 60
           MGS N ES+DE G SAIRA+GSLFKLTEVFLWDDETE ARRVESSLA DADD NNE+F E
Sbjct: 1   MGSSNEESEDEAGVSAIRAIGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  KICSTISDISLQPEDVELTEQMNALGLPLSFHTNKERKRIGINMGKRKATVKHSRIQPEL 120
           KICSTI+DISL PED++LTEQMNALGLPLSFHTNKE++R GI MGKRK TVKHSRIQ   
Sbjct: 61  KICSTITDISLSPEDIQLTEQMNALGLPLSFHTNKEQRRTGITMGKRKTTVKHSRIQHGF 120

Query: 121 LDKEMEFPKVSSKQEVVANTDFNNDTTGSLCCSSMVDQSETSDC---------NVFFGGD 180
           LDKE+EFPK SS+ E+VAN + N++  GSLCCSSMV+QSE SDC         +V F GD
Sbjct: 121 LDKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180

Query: 181 ISPDSTGLLSGAVEELSSHVKFDCLLNNEEDHELNLDNAMLGDHMKVRSNSIGLDKDPSP 240
           ISP+S+GL+ GAVEE S +VK D +LNN  DHE    +A+LGDH KVR + IGLDK  SP
Sbjct: 181 ISPNSSGLIHGAVEEQSCNVKCDIVLNNRGDHESG--DALLGDHAKVRLSPIGLDKGHSP 240

Query: 241 IKCMIGLDVSHTKLEEVEPPMESEGSSTTSEDTEVQKIDIDIGIGLPLLAESSFLHMGAD 300
             CM G DVSH K EEVE PME EGSSTT +DTEVQKIDID GIGLPL+AE S+LHMGAD
Sbjct: 241 RICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSYLHMGAD 300

Query: 301 YSENDHVVGCIHESGEWAVYWDSFYMRNYFYNLKTHESTWNPPPGLEHFAVSDANCTENE 360
           Y+ENDHVVGCI E GEW VYWDSFYMRNYFYN+KTHESTWNPPPGLEHFA SDAN TENE
Sbjct: 301 YNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHSDANFTENE 360

Query: 361 SIAEVAEMDVLEDVKSEDICSVLGDTRSCVNLLGDNAHCQPPDGLL-------EGSETSA 420
           SIAEVAEMDVLED K EDICSVL DTRSC+NL GDN HCQPPD LL       EGS++ A
Sbjct: 361 SIAEVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKSRA 420

Query: 421 SVHTLTNSHKHSDEPHEWQTSCRNTREDI--------------RCSNDFQFTVANGAFEQ 480
           SVHT  NS+   DEPHEW TSCRNTRE I               CSN FQ  VAN   EQ
Sbjct: 421 SVHTSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANETSEQ 480

Query: 481 KTFSLGKLSNVDLPEIDCITRDDGDDESTMGLYPSSVSHMLQQAVHRDGDMHFGNGPTIG 540
           KTFS  K SN+D PE   +T    DDE  +GL  SSVSH+LQQA H DGDMHFGN PTI 
Sbjct: 481 KTFSHCKSSNMDSPEKAFVTI---DDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTIC 540

Query: 541 TLVTEQNLAGRNRKKKMKRTRRHRQLFDRNEEFQSLAITEEYPTSIAKYWCQRYQLFSRF 600
           TL TEQNL+GR+RKKKMKRTRR  QL DRNEEF SLAITEEYPTSI KYWCQRYQLFSRF
Sbjct: 541 TLGTEQNLSGRDRKKKMKRTRRRGQLSDRNEEFHSLAITEEYPTSITKYWCQRYQLFSRF 600

Query: 601 DDGVKMDKEGWFSVTPEPIARHHALRCSSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
           DDGVKMDKEGWFSVTPE IARHHA RC SNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP
Sbjct: 601 DDGVKMDKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660

Query: 661 IKIRYAQHNAAIYGVEDQIDFVKGDFFCLAPSLKADVIFLSPPWGGPDYAKVDIYDLKTM 720
           IKIRYAQHNAA+YGVEDQIDF+KGDFF LAP LKADVIFLSPPWGGP+YA+VDIYDLKTM
Sbjct: 661 IKIRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTM 720

Query: 721 LKPHDGYFLFNIAKKVAPLVVMFLPRNVNFDQLAELSLSSDPPWSLEVEKNFLNGKLKAI 762
           L+PHDGYFLFNIAKK+AP+VVMFLP+NVN DQLAE++LSS+PPWSLEVEKNFLNGKLKAI
Sbjct: 721 LRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAI 780

BLAST of Lag0036955 vs. ExPASy TrEMBL
Match: A0A6J1IFL4 (Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=1)

HSP 1 Score: 1172.9 bits (3033), Expect = 0.0e+00
Identity = 602/791 (76.11%), Postives = 651/791 (82.30%), Query Frame = 0

Query: 1   MGSGNGESDDEVGASAIRALGSLFKLTEVFLWDDETETARRVESSLAPDADDTNNEEFGE 60
           MGS N ES+DE G SAIRA+GSLFKLTEVFLWDDETE ARRVESSLA DADD NNE+F E
Sbjct: 1   MGSSNEESEDEAGVSAIRAIGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  KICSTISDISLQPEDVELTEQMNALGLPLSFHTNKERKRIGINMGKRKATVKHSRIQPEL 120
           KICSTI+DISL PED++LTEQMNALGLPLSFHTNKER R GI MGKRK TVKHSRIQ   
Sbjct: 61  KICSTITDISLSPEDIQLTEQMNALGLPLSFHTNKER-RTGITMGKRKTTVKHSRIQHGF 120

Query: 121 LDKEMEFPKVSSKQEVVANTDFNNDTTGSLCCSSMVDQSETSDC---------NVFFGGD 180
           LDKE+EFPK SS+ E+VAN + N++  GSLCCSSMV+QSE SDC         +V F GD
Sbjct: 121 LDKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180

Query: 181 ISPDSTGLLSGAVEELSSHVKFDCLLNNEEDHELNLDNAMLGDHMKVRSNSIGLDKDPSP 240
           ISP+S+GL+ GAVEE S +VK D +LNN  DHE    +A+LGDH KVR + IGLDK  SP
Sbjct: 181 ISPNSSGLIHGAVEEQSCNVKCDIVLNNRGDHESG--DALLGDHAKVRLSPIGLDKGHSP 240

Query: 241 IKCMIGLDVSHTKLEEVEPPMESEGSSTTSEDTEVQKIDIDIGIGLPLLAESSFLHMGAD 300
             CM G DVSH K EEVE PME EGSSTT +DTEVQKIDID GIGLPL+AE S+LHMGAD
Sbjct: 241 RICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSYLHMGAD 300

Query: 301 YSENDHVVGCIHESGEWAVYWDSFYMRNYFYNLKTHESTWNPPPGLEHFAVSDANCTENE 360
           Y+ENDHVVGCI E GEW VYWDSFYMRNYFYN+KTHESTWNPPPGLEHFA SDAN TENE
Sbjct: 301 YNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHSDANFTENE 360

Query: 361 SIAEVAEMDVLEDVKSEDICSVLGDTRSCVNLLGDNAHCQPPDGLL-------EGSETSA 420
           SIAEVAEMDVLED K EDICSVL DTRSC+NL GDN HCQPPD LL       EGS++ A
Sbjct: 361 SIAEVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKSRA 420

Query: 421 SVHTLTNSHKHSDEPHEWQTSCRNTREDI--------------RCSNDFQFTVANGAFEQ 480
           SVHT  NS+   DEPHEW TSCRNTRE I               CSN FQ  VAN   EQ
Sbjct: 421 SVHTSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANETSEQ 480

Query: 481 KTFSLGKLSNVDLPEIDCITRDDGDDESTMGLYPSSVSHMLQQAVHRDGDMHFGNGPTIG 540
           KTFS  K SN+D PE   +T    DDE  +GL  SSVSH+LQQA H DGDMHFGN PTI 
Sbjct: 481 KTFSHCKSSNMDSPEKAFVTI---DDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTIC 540

Query: 541 TLVTEQNLAGRNRKKKMKRTRRHRQLFDRNEEFQSLAITEEYPTSIAKYWCQRYQLFSRF 600
           TL TEQNL+GR+RKKKMKRTRR  QL DRNEEF SLAITEEYPTSI KYWCQRYQLFSRF
Sbjct: 541 TLGTEQNLSGRDRKKKMKRTRRRGQLSDRNEEFHSLAITEEYPTSITKYWCQRYQLFSRF 600

Query: 601 DDGVKMDKEGWFSVTPEPIARHHALRCSSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
           DDGVKMDKEGWFSVTPE IARHHA RC SNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP
Sbjct: 601 DDGVKMDKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660

Query: 661 IKIRYAQHNAAIYGVEDQIDFVKGDFFCLAPSLKADVIFLSPPWGGPDYAKVDIYDLKTM 720
           IKIRYAQHNAA+YGVEDQIDF+KGDFF LAP LKADVIFLSPPWGGP+YA+VDIYDLKTM
Sbjct: 661 IKIRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTM 720

Query: 721 LKPHDGYFLFNIAKKVAPLVVMFLPRNVNFDQLAELSLSSDPPWSLEVEKNFLNGKLKAI 762
           L+PHDGYFLFNIAKK+AP+VVMFLP+NVN DQLAE++LSS+PPWSLEVEKNFLNGKLKAI
Sbjct: 721 LRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAI 780

BLAST of Lag0036955 vs. ExPASy TrEMBL
Match: A0A6J1C9F8 (Trimethylguanosine synthase OS=Momordica charantia OX=3673 GN=LOC111009535 PE=4 SV=1)

HSP 1 Score: 1160.6 bits (3001), Expect = 0.0e+00
Identity = 589/777 (75.80%), Postives = 647/777 (83.27%), Query Frame = 0

Query: 1   MGSGNGESDDEVGASAIRALGSLFKLTEVFLWDDETETARRVESSLAPDADDTNNEEFGE 60
           MG  NGES+DEVGA AIRALG LFKLTEVFLWDDETE ARR E SLAPDADDT+NE+ GE
Sbjct: 1   MGFDNGESEDEVGAPAIRALGPLFKLTEVFLWDDETEAARRAERSLAPDADDTHNEDLGE 60

Query: 61  KICSTISDISLQPEDVELTEQMNALGLPLSFHTNKERKRIGINMGKRKATVKHSRIQPEL 120
           K+CSTIS ISL PED ELT+QMNALGLPLSFHTNKE KR GI  GKR+AT+KHSRIQ  L
Sbjct: 61  KVCSTISGISLLPEDTELTQQMNALGLPLSFHTNKE-KRNGITTGKRRATIKHSRIQQGL 120

Query: 121 LDKEMEFPKVSSKQEVVANTDFNNDTTGSLCCSSMVDQSETSDCNVFFGGDISPDSTGLL 180
           LDKEMEFP+VSS +E+VAN +FN+D  GSLCC+SMVDQSE SDC+VF  GDI P+STGL+
Sbjct: 121 LDKEMEFPRVSSGEEIVANLNFNDDAIGSLCCASMVDQSEASDCHVFLDGDILPNSTGLI 180

Query: 181 SGAVEELSSHVKFDCLLNNEEDHELNLDNAMLGDHMKVRSNSIGLDKDPSPIKCMIGLDV 240
           S  VEE S ++  D LLNN EDHE +  NA+LG+H  V S+SI LDK  SP + MIG+DV
Sbjct: 181 SSTVEEQSHNLMIDSLLNNGEDHESSPGNAVLGNHSIVTSSSISLDKVHSPKRYMIGIDV 240

Query: 241 SHTKLEEVEPPMESEGSSTTSEDTEVQKIDIDIGIGLPLLAESSFLHMGADYSENDHVVG 300
           SH+  E +EPPME EGSS T +DTEVQKI+ID GIGLPL+AESS LHM A Y ENDHVVG
Sbjct: 241 SHS--EALEPPMELEGSSMTLQDTEVQKINIDSGIGLPLVAESSLLHMEAGYDENDHVVG 300

Query: 301 CIHESGEWAVYWDSFYMRNYFYNLKTHESTWNPPPGLEHFAVSDANCTENESIAEVAEMD 360
           CIHE GEW VYWDSFYMRNYFYN+K+HESTWNPP GLEHFA+SDA+CT NESIAEVAEMD
Sbjct: 301 CIHEFGEWMVYWDSFYMRNYFYNIKSHESTWNPPAGLEHFALSDADCTANESIAEVAEMD 360

Query: 361 VLEDVKSEDICSVLGDTRSCVNLLGDNAHCQPPDGLLEGSETSASVHTLTNSHKHSDEPH 420
           V+EDVKS+DICSVLG+T S           +    L+E  ETS  ++T TNSHKHSD+PH
Sbjct: 361 VVEDVKSQDICSVLGETLS-----------EGSSILIESPETSGFINTSTNSHKHSDDPH 420

Query: 421 EWQTSCRNTREDI--------------RCSNDFQFTVANGAFEQKTFSLGKLSNVDLPEI 480
           + QTSCRNTR+DI               CSNDFQ T ANGA EQKTFSLGK SN+D PEI
Sbjct: 421 DCQTSCRNTRKDIGCSCEGHVKQLCREHCSNDFQLTFANGALEQKTFSLGKPSNIDSPEI 480

Query: 481 DCITRDD-GDDESTMGLYPSSVSHMLQQAVHRDGDMHFGNGPTIGTLVTEQNLAGRNRKK 540
           D +TRDD  DDE TM +Y SSVSHM+QQA  RD  M FGNG TI TL  E+NLAG +RKK
Sbjct: 481 DIVTRDDYDDDEGTMSIYTSSVSHMIQQADQRDSGMDFGNGRTICTLGAEKNLAGISRKK 540

Query: 541 KMKRTRRHRQLFDRNEEFQSLAITEEYPTSIAKYWCQRYQLFSRFDDGVKMDKEGWFSVT 600
           KMKR RR RQL +RNEEFQSLAITEEYPTSIAKYWCQRYQLFSRFDDGVKMD+EGWFSVT
Sbjct: 541 KMKRIRRRRQLSERNEEFQSLAITEEYPTSIAKYWCQRYQLFSRFDDGVKMDEEGWFSVT 600

Query: 601 PEPIARHHALRCSSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPIKIRYAQHNAAIYGV 660
           PEPIA+HHALRC  N+I+DSFTGVGGNAIQFSQRAKHVIAIDIDP KIRYA HNA+IYGV
Sbjct: 601 PEPIAQHHALRCGCNLIVDSFTGVGGNAIQFSQRAKHVIAIDIDPRKIRYAHHNASIYGV 660

Query: 661 EDQIDFVKGDFFCLAPSLKADVIFLSPPWGGPDYAKVDIYDLKTMLKPHDGYFLFNIAKK 720
           EDQIDF+KGDFF LAPSLKADVIFLSPPWGGPDY KVD YDLKTMLKPHDGYFLFNIAKK
Sbjct: 661 EDQIDFIKGDFFRLAPSLKADVIFLSPPWGGPDYTKVDTYDLKTMLKPHDGYFLFNIAKK 720

Query: 721 VAPLVVMFLPRNVNFDQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYFSNGSMNKP 763
           +AP VVMFLPRNVN DQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYFSNGS N+P
Sbjct: 721 IAPFVVMFLPRNVNVDQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYFSNGSTNEP 763

BLAST of Lag0036955 vs. ExPASy TrEMBL
Match: A0A6J1FD09 (Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 SV=1)

HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 592/791 (74.84%), Postives = 642/791 (81.16%), Query Frame = 0

Query: 1   MGSGNGESDDEVGASAIRALGSLFKLTEVFLWDDETETARRVESSLAPDADDTNNEEFGE 60
           MGSGN ES  E G SAIRALGSLFKLTEVFLWDDETE ARRVESSLA DADD NNE+F E
Sbjct: 1   MGSGNEES--EAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  KICSTISDISLQPEDVELTEQMNALGLPLSFHTNKERKRIGINMGKRKATVKHSRIQPEL 120
           KICSTI+DISL PED+ELTEQMNALGLPLSFHTNKE++R GI MGKR  TVKHSRIQ   
Sbjct: 61  KICSTITDISLSPEDIELTEQMNALGLPLSFHTNKEQRRTGITMGKRNTTVKHSRIQQGF 120

Query: 121 LDKEMEFPKVSSKQEVVANTDFNNDTTGSLCCSSMVDQSETSDC---------NVFFGGD 180
           LDKE+EFPK SS+ E+VAN + N++  GSLCCSSMV+QSE SDC         +V F GD
Sbjct: 121 LDKEVEFPKSSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180

Query: 181 ISPDSTGLLSGAVEELSSHVKFDCLLNNEEDHELNLDNAMLGDHMKVRSNSIGLDKDPSP 240
           ISP+S+GL+ GA EE S  V  D +LNN  DHE    +A+LGDH KVR +SIGLDK  SP
Sbjct: 181 ISPNSSGLIHGAFEEQSCDVTCDIVLNNRGDHESG--DAVLGDHAKVRLSSIGLDKGHSP 240

Query: 241 IKCMIGLDVSHTKLEEVEPPMESEGSSTTSEDTEVQKIDIDIGIGLPLLAESSFLHMGAD 300
             CM G DVSH K EEVE PME EGSSTT +DTEVQKIDID GIGLPL+AE SFLHMGAD
Sbjct: 241 RICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMGAD 300

Query: 301 YSENDHVVGCIHESGEWAVYWDSFYMRNYFYNLKTHESTWNPPPGLEHFAVSDANCTENE 360
           Y+ENDHVVGCI E GEW VYWDSFYMRNYFYN+KTHESTWNPP GLEHFA  DAN TENE
Sbjct: 301 YNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENE 360

Query: 361 SIAEVAEMDVLEDVKSEDICSVLGDTRSCVNLLGDNAHCQPPDGLL-------EGSETSA 420
           SIAEVAEMDVLED+K EDICSVL DTRSC+NL GDN HCQPPD LL       EGS+  A
Sbjct: 361 SIAEVAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRA 420

Query: 421 SVHTLTNSHKHSDEPHEWQTSCRNTREDI--------------RCSNDFQFTVANGAFEQ 480
           SV+T  NS+   DEPHEW T+ RNTRE I               CSN FQ  VAN A EQ
Sbjct: 421 SVNTSINSYMQPDEPHEWLTNRRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQ 480

Query: 481 KTFSLGKLSNVDLPEIDCITRDDGDDESTMGLYPSSVSHMLQQAVHRDGDMHFGNGPTIG 540
           KTFS  K SN+  PE   +T    DDE  + L  SSVSH+LQQA H +GDMHFGN PTI 
Sbjct: 481 KTFSHCKPSNMYSPEKAFVTI---DDEGAVDLTTSSVSHVLQQADHMNGDMHFGNEPTIC 540

Query: 541 TLVTEQNLAGRNRKKKMKRTRRHRQLFDRNEEFQSLAITEEYPTSIAKYWCQRYQLFSRF 600
           TL TEQNL+GR+RKKKMKRTRR  Q  DRNEEF S AITEEYPTSI KYWCQRYQLFSRF
Sbjct: 541 TLGTEQNLSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRF 600

Query: 601 DDGVKMDKEGWFSVTPEPIARHHALRCSSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
           DDGVKMDKEGWFSVTPE IARHHA RC SNMI+DSFTGVGGNAIQFSQRAKHVIAIDIDP
Sbjct: 601 DDGVKMDKEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDP 660

Query: 661 IKIRYAQHNAAIYGVEDQIDFVKGDFFCLAPSLKADVIFLSPPWGGPDYAKVDIYDLKTM 720
            KIRYAQHNAA+YGVEDQIDF+KGDFF LAP LKADVIFLSPPWGGP+YA+VDIYDLKTM
Sbjct: 661 TKIRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTM 720

Query: 721 LKPHDGYFLFNIAKKVAPLVVMFLPRNVNFDQLAELSLSSDPPWSLEVEKNFLNGKLKAI 762
           L+PHDGYFLFNIAKK+AP+VVMFLP+NVN DQLAE++LSS+PPWSLEVEKNFLNGKLKAI
Sbjct: 721 LRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAI 780

BLAST of Lag0036955 vs. ExPASy TrEMBL
Match: A0A6J1FDE5 (Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 SV=1)

HSP 1 Score: 1139.0 bits (2945), Expect = 0.0e+00
Identity = 593/791 (74.97%), Postives = 641/791 (81.04%), Query Frame = 0

Query: 1   MGSGNGESDDEVGASAIRALGSLFKLTEVFLWDDETETARRVESSLAPDADDTNNEEFGE 60
           MGSGN ES  E G SAIRALGSLFKLTEVFLWDDETE ARRVESSLA DADD NNE+F E
Sbjct: 1   MGSGNEES--EAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  KICSTISDISLQPEDVELTEQMNALGLPLSFHTNKERKRIGINMGKRKATVKHSRIQPEL 120
           KICSTI+DISL PED+ELTEQMNALGLPLSFHTNKER R GI MGKR  TVKHSRIQ   
Sbjct: 61  KICSTITDISLSPEDIELTEQMNALGLPLSFHTNKER-RTGITMGKRNTTVKHSRIQQGF 120

Query: 121 LDKEMEFPKVSSKQEVVANTDFNNDTTGSLCCSSMVDQSETSDC---------NVFFGGD 180
           LDKE+EFPK SS+ E+VAN + N++  GSLCCSSMV+QSE SDC         +V F GD
Sbjct: 121 LDKEVEFPKSSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180

Query: 181 ISPDSTGLLSGAVEELSSHVKFDCLLNNEEDHELNLDNAMLGDHMKVRSNSIGLDKDPSP 240
           ISP+S+GL+ GA EE S  V  D +LNN  DHE    +A+LGDH KVR +SIGLDK  SP
Sbjct: 181 ISPNSSGLIHGAFEEQSCDVTCDIVLNNRGDHESG--DAVLGDHAKVRLSSIGLDKGHSP 240

Query: 241 IKCMIGLDVSHTKLEEVEPPMESEGSSTTSEDTEVQKIDIDIGIGLPLLAESSFLHMGAD 300
             CM G DVSH K EEVE PME EGSSTT +DTEVQKIDID GIGLPL+AE SFLHMGAD
Sbjct: 241 RICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMGAD 300

Query: 301 YSENDHVVGCIHESGEWAVYWDSFYMRNYFYNLKTHESTWNPPPGLEHFAVSDANCTENE 360
           Y+ENDHVVGCI E GEW VYWDSFYMRNYFYN+KTHESTWNPP GLEHFA  DAN TENE
Sbjct: 301 YNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENE 360

Query: 361 SIAEVAEMDVLEDVKSEDICSVLGDTRSCVNLLGDNAHCQPPDGLL-------EGSETSA 420
           SIAEVAEMDVLED+K EDICSVL DTRSC+NL GDN HCQPPD LL       EGS+  A
Sbjct: 361 SIAEVAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRA 420

Query: 421 SVHTLTNSHKHSDEPHEWQTSCRNTREDI--------------RCSNDFQFTVANGAFEQ 480
           SV+T  NS+   DEPHEW T+ RNTRE I               CSN FQ  VAN A EQ
Sbjct: 421 SVNTSINSYMQPDEPHEWLTNRRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQ 480

Query: 481 KTFSLGKLSNVDLPEIDCITRDDGDDESTMGLYPSSVSHMLQQAVHRDGDMHFGNGPTIG 540
           KTFS  K SN+  PE   +T    DDE  + L  SSVSH+LQQA H +GDMHFGN PTI 
Sbjct: 481 KTFSHCKPSNMYSPEKAFVTI---DDEGAVDLTTSSVSHVLQQADHMNGDMHFGNEPTIC 540

Query: 541 TLVTEQNLAGRNRKKKMKRTRRHRQLFDRNEEFQSLAITEEYPTSIAKYWCQRYQLFSRF 600
           TL TEQNL+GR+RKKKMKRTRR  Q  DRNEEF S AITEEYPTSI KYWCQRYQLFSRF
Sbjct: 541 TLGTEQNLSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRF 600

Query: 601 DDGVKMDKEGWFSVTPEPIARHHALRCSSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
           DDGVKMDKEGWFSVTPE IARHHA RC SNMI+DSFTGVGGNAIQFSQRAKHVIAIDIDP
Sbjct: 601 DDGVKMDKEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDP 660

Query: 661 IKIRYAQHNAAIYGVEDQIDFVKGDFFCLAPSLKADVIFLSPPWGGPDYAKVDIYDLKTM 720
            KIRYAQHNAA+YGVEDQIDF+KGDFF LAP LKADVIFLSPPWGGP+YA+VDIYDLKTM
Sbjct: 661 TKIRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTM 720

Query: 721 LKPHDGYFLFNIAKKVAPLVVMFLPRNVNFDQLAELSLSSDPPWSLEVEKNFLNGKLKAI 762
           L+PHDGYFLFNIAKK+AP+VVMFLP+NVN DQLAE++LSS+PPWSLEVEKNFLNGKLKAI
Sbjct: 721 LRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAI 780

BLAST of Lag0036955 vs. TAIR 10
Match: AT1G45231.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 359.0 bits (920), Expect = 9.5e-99
Identity = 269/758 (35.49%), Postives = 355/758 (46.83%), Query Frame = 0

Query: 1   MGSGNGESDDEVGASAIRALGSLFKLTEVFLWDDETETARRVESSLAPDADDTNNEEFGE 60
           MG   GE ++  G  AI ALGSLFKLT++ LW D T+T    + S     DDT    +G 
Sbjct: 1   MGKVRGEVEEAEG-EAIEALGSLFKLTQIHLWVD-TDTPLFHQHSGNKSVDDT----YG- 60

Query: 61  KICSTISDISLQPEDVELTEQMNALGLPLSFHTNKERKRIGINMGKRKATVKHSRIQPEL 120
                I++  L  E       MN LGLP+SF TNK+ K      G +K   K      + 
Sbjct: 61  -----IANNGLMKE-------MNDLGLPVSFRTNKQWK--NRTRGYQKKGFK------DR 120

Query: 121 LDKEMEFPKVSSKQEVVANTDFNNDTTGSLCCSSMVDQSETSDCNVFFGGDISPDSTGLL 180
           LD+E+          V+   +                                 D+  L+
Sbjct: 121 LDEEV---------NVILKVE---------------------------------DTLNLV 180

Query: 181 SGAVEELSSH-VKFDC---LLNNEEDHELNLDNAMLGDHMKVRSNSIGLDKDPSPIKCMI 240
           S   EE+ S   + DC   +   EE+HE+ + +  LG+                      
Sbjct: 181 SALNEEVESPCFEEDCVQVIEVEEENHEVVVGSCFLGN---------------------- 240

Query: 241 GLDVSHTKLEEVEPPMESEGSSTTSEDTEVQKIDIDIGIGLPLLAESSFLHMGADYSEND 300
                             +G S  + +T      ID                        
Sbjct: 241 -----------------GDGDSVLASET------ID------------------------ 300

Query: 301 HVVGCIHESGEWAVYWDSFYMRNYFYNLKTHESTWNPPPGLEHFAVSDANCTENESIAEV 360
                 H+S  W VYWDSFY R+YFYN KT ES W PP G+EH A SD +   +E + E 
Sbjct: 301 -----SHDSSVWKVYWDSFYGRSYFYNFKTQESKWEPPLGMEHLAYSDESHNLSELVIEK 360

Query: 361 AEMDVLEDVKSEDICSVLGDTRSCVNLLGDNAHCQPPDGLLEGSETSASVHTLTNSHKHS 420
              D+   V  +D+     D    V        CQ     LE +E +  V++L ++++  
Sbjct: 361 HHDDLSGTVLGDDVPFDKADDLGGV--------CQ---SQLE-AEATEEVNSLIDTYQ-- 420

Query: 421 DEPHEWQTSCRNTREDIRCSNDFQFTVANGAFEQKTFSLGKLSNVDLPEIDCITRDDGDD 480
                 +TS  N   DI                    SLG                   +
Sbjct: 421 ------ETSIGNQSLDIT-------------------SLG-------------------E 480

Query: 481 ESTMGLYPSSVSHMLQQAVHRDGDMHFGNGPTIGTLVTEQNLAGRNRKKKMKRTRRHRQL 540
           E T     SSV                                 R  KK+ +R+R  ++L
Sbjct: 481 EGTGAYVVSSV---------------------------------RKAKKESRRSRAKKKL 524

Query: 541 FDRNEEFQSLAITEEYPTSIAKYWCQRYQLFSRFDDGVKMDKEGWFSVTPEPIARHHALR 600
            +     +   + EEY   + KYWCQRY LFSRFD+G+KMD+EGWFSVTPE IA+HHA R
Sbjct: 541 LNSYTGTEMKGVPEEYSPILGKYWCQRYLLFSRFDEGIKMDEEGWFSVTPELIAKHHATR 524

Query: 601 CSSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPIKIRYAQHNAAIYGVEDQIDFVKGDF 660
           C+  ++ID FTGVGGNAIQF+ R+ +VIAID+DP K+  A+HNAAIYGV D+IDFVKGDF
Sbjct: 601 CNEGIVIDCFTGVGGNAIQFASRSHYVIAIDLDPKKLDLAKHNAAIYGVADKIDFVKGDF 524

Query: 661 FCLAPSLKADVIFLSPPWGGPDYAKVDIYDLKTMLKPHDGYFLFNIAKKVAPLVVMFLPR 720
           F LA +LKA  +FLSPPWGGPDY K   YD+KTML+P DG  LF  A  +A  ++MFLPR
Sbjct: 661 FDLAHNLKAGTVFLSPPWGGPDYLKASTYDMKTMLRPRDGDALFKAAMNIASTIIMFLPR 524

Query: 721 NVNFDQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYF 755
           NV+ +QLAEL+LS+ PPWSLEVEKN+LNGKLKA+TAY+
Sbjct: 721 NVDINQLAELALSTSPPWSLEVEKNYLNGKLKAVTAYY 524

BLAST of Lag0036955 vs. TAIR 10
Match: AT1G30550.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 258.5 bits (659), Expect = 1.7e-68
Identity = 123/218 (56.42%), Postives = 163/218 (74.77%), Query Frame = 0

Query: 541 EEFQSLAITEEYPTS--IAKYWCQRYQLFSRFDDGVKMDKEGWFSVTPEPIARHHALRCS 600
           +E +SL I +E+ T+  I++YW QRY LFS++D G++MD+EGW+SVTPE IA   A RC 
Sbjct: 3   KEAESL-IEKEHGTNPKISRYWIQRYDLFSKYDQGIEMDEEGWYSVTPEEIAIKQAERCR 62

Query: 601 SNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPIKIRYAQHNAAIYGVEDQIDFVKGDFFC 660
             ++ID F+GVGGN IQF++    VIAIDIDP+KI  A +NA +YGV ++IDFV GDF  
Sbjct: 63  GKVVIDCFSGVGGNTIQFAKVCSSVIAIDIDPMKIALAMNNAKVYGVANRIDFVTGDFMQ 122

Query: 661 LAPSLKADVIFLSPPWGGPDYAKVDIYDLKTMLKPHDGYFLFNIAKKVAPLVVMFLPRNV 720
           LAPSLK DV+FLSPPWGGP Y+KV+ Y L  ML P DGY LF  A  + P ++MFLP+N+
Sbjct: 123 LAPSLKGDVLFLSPPWGGPTYSKVESYKL-DMLLPRDGYSLFQTALSITPNIIMFLPKNI 182

Query: 721 NFDQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYFSN 757
           +  QL EL+  S PP +LE+E+N + G++KAITAYFS+
Sbjct: 183 DLAQLEELACLSSPPLTLEIEENSIGGEIKAITAYFSS 218

BLAST of Lag0036955 vs. TAIR 10
Match: AT1G30550.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 256.1 bits (653), Expect = 8.7e-68
Identity = 115/200 (57.50%), Postives = 151/200 (75.50%), Query Frame = 0

Query: 556 IAKYWCQRYQLFSRFDDGVKMDKEGWFSVTPEPIARHHALRCSSNMIIDSFTGVGGNAIQ 615
           I KYW QRY LFSR+D G++MD+EGW+SVTPE IA   A R    ++ID F+GVGGN IQ
Sbjct: 253 ITKYWIQRYDLFSRYDQGIEMDEEGWYSVTPEEIAIKQAQRYRGKVVIDCFSGVGGNTIQ 312

Query: 616 FSQRAKHVIAIDIDPIKIRYAQHNAAIYGVEDQIDFVKGDFFCLAPSLKADVIFLSPPWG 675
           F++    V+AIDIDP+K+  A +NA +YGV +++DFV GDF  LAPSLK DV+FLSPPWG
Sbjct: 313 FAKVCSSVVAIDIDPVKVELAMNNAMVYGVANRVDFVIGDFIQLAPSLKGDVVFLSPPWG 372

Query: 676 GPDYAKVDIYDLKTMLKPHDGYFLFNIAKKVAPLVVMFLPRNVNFDQLAELSLSSDPPWS 735
           GP Y   + Y+L  ML+P DGY LF IA+ + P ++MFLPRNV+  Q+ EL+  S PP +
Sbjct: 373 GPMYRDFESYNL-DMLQPRDGYSLFQIAQSITPNIIMFLPRNVDLAQVEELAWLSSPPLN 432

Query: 736 LEVEKNFLNGKLKAITAYFS 756
           LE+E+NF+ G++KA+TAYFS
Sbjct: 433 LEIEENFVGGRMKAVTAYFS 451

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022975951.10.0e+0075.98uncharacterized protein LOC111476503 isoform X1 [Cucurbita maxima] >XP_022975952... [more]
XP_022975956.10.0e+0076.11uncharacterized protein LOC111476503 isoform X2 [Cucurbita maxima][more]
XP_023535334.10.0e+0075.60uncharacterized protein LOC111796805 isoform X1 [Cucurbita pepo subsp. pepo] >XP... [more]
XP_022138324.10.0e+0075.80uncharacterized protein LOC111009535 isoform X1 [Momordica charantia] >XP_022138... [more]
XP_023535337.10.0e+0075.73uncharacterized protein LOC111796805 isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
P851073.2e-6752.94Trimethylguanosine synthase OS=Rattus norvegicus OX=10116 GN=Tgs1 PE=1 SV=1[more]
Q923W13.5e-6652.12Trimethylguanosine synthase OS=Mus musculus OX=10090 GN=Tgs1 PE=1 SV=2[more]
Q96RS06.7e-6551.26Trimethylguanosine synthase OS=Homo sapiens OX=9606 GN=TGS1 PE=1 SV=3[more]
Q098143.1e-3839.74Trimethylguanosine synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 2484... [more]
Q120521.9e-2740.45Trimethylguanosine synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S2... [more]
Match NameE-valueIdentityDescription
A0A6J1II650.0e+0075.98Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=... [more]
A0A6J1IFL40.0e+0076.11Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=... [more]
A0A6J1C9F80.0e+0075.80Trimethylguanosine synthase OS=Momordica charantia OX=3673 GN=LOC111009535 PE=4 ... [more]
A0A6J1FD090.0e+0074.84Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 S... [more]
A0A6J1FDE50.0e+0074.97Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT1G45231.29.5e-9935.49S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G30550.21.7e-6856.42S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G30550.18.7e-6857.50S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019012RNA cap guanine-N2 methyltransferasePFAMPF09445Methyltransf_15coord: 600..755
e-value: 4.0E-40
score: 137.1
NoneNo IPR availableGENE3D2.20.70.10coord: 301..352
e-value: 7.8E-6
score: 27.4
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 549..761
e-value: 6.4E-80
score: 269.8
NoneNo IPR availablePANTHERPTHR14741S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE RELATEDcoord: 24..757
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 602..700
e-value: 2.65922E-9
score: 53.2027
IPR001202WW domainPROSITEPS01159WW_DOMAIN_1coord: 308..334
IPR001202WW domainPROSITEPS50020WW_DOMAIN_2coord: 308..336
score: 10.3837
IPR001202WW domainCDDcd00201WWcoord: 308..336
e-value: 3.69425E-4
score: 36.3518
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 548..720
IPR036020WW domain superfamilySUPERFAMILY51045WW domaincoord: 300..335

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0036955.1Lag0036955.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009452 7-methylguanosine RNA capping
biological_process GO:0001510 RNA methylation
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0005515 protein binding