Lag0036935 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0036935 vs. NCBI nr
Match: XP_038898530.1 (probable carboxylesterase 2 [Benincasa hispida])

HSP 1 Score: 274.2 bits (700), Expect = 6.4e-70
Identity = 125/148 (84.46%), Postives = 140/148 (94.59%), Query Frame = 0

Query: 1   MALRAKNSDLAGKIKILGIALIQPYFWGQEPIGSEITEHEQKAQVDRWWNFVCPSDGGND 60
           MALRAK+S+L  KIK++GIALIQPYFWGQEPIGSEITEH ++A+VDRWWNFVCPSD GND
Sbjct: 179 MALRAKSSNLGAKIKVVGIALIQPYFWGQEPIGSEITEHHKRAEVDRWWNFVCPSDRGND 238

Query: 61  DLLINPFADGSPAIEGLAGEKAVVLVAEKDILRDRGRLYYENLAKSEWKGKVEIFETEGE 120
           DLLINPF+DGSPAI+GL GEK VV+VAEKDILRDRG+LYYENLAKSEWKGKVEI+ETEGE
Sbjct: 239 DLLINPFSDGSPAIDGLTGEKVVVIVAEKDILRDRGKLYYENLAKSEWKGKVEIYETEGE 298

Query: 121 DHAFHILDPTSEKAKSLTKRLALFINED 149
           DHAFH+LDPTSEKAK+L KRLA F+NED
Sbjct: 299 DHAFHMLDPTSEKAKALIKRLAFFLNED 326

BLAST of Lag0036935 vs. NCBI nr
Match: XP_023525571.1 (probable carboxylesterase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 267.3 bits (682), Expect = 7.8e-68
Identity = 124/148 (83.78%), Postives = 135/148 (91.22%), Query Frame = 0

Query: 1   MALRAKNSDLAGKIKILGIALIQPYFWGQEPIGSEITEHEQKAQVDRWWNFVCPSDGGND 60
           MALRA+NS+LAGK KI GIALIQPYFWG+EPIGSEITE E+KA VD WWNFVCPSD GND
Sbjct: 182 MALRAQNSNLAGKFKIRGIALIQPYFWGEEPIGSEITEPEKKALVDSWWNFVCPSDRGND 241

Query: 61  DLLINPFADGSPAIEGLAGEKAVVLVAEKDILRDRGRLYYENLAKSEWKGKVEIFETEGE 120
           DLLINPFADGSP++EGL GEK VV+VAEKDILRDRG +YYENLAKS WKGKV+IFETEGE
Sbjct: 242 DLLINPFADGSPSVEGLVGEKVVVIVAEKDILRDRGMIYYENLAKSNWKGKVDIFETEGE 301

Query: 121 DHAFHILDPTSEKAKSLTKRLALFINED 149
           DHAFH+LDPTSEKAK L  RLALF+NED
Sbjct: 302 DHAFHLLDPTSEKAKRLVNRLALFVNED 329

BLAST of Lag0036935 vs. NCBI nr
Match: XP_022982269.1 (probable carboxylesterase 2 isoform X1 [Cucurbita maxima])

HSP 1 Score: 266.5 bits (680), Expect = 1.3e-67
Identity = 125/148 (84.46%), Postives = 134/148 (90.54%), Query Frame = 0

Query: 1   MALRAKNSDLAGKIKILGIALIQPYFWGQEPIGSEITEHEQKAQVDRWWNFVCPSDGGND 60
           MALRA+NS LAGK KI GIALIQPYFWG+EPIGSEITE E+KA VD WWNFVCPSD GND
Sbjct: 182 MALRAQNSHLAGKFKIRGIALIQPYFWGREPIGSEITEPEKKALVDGWWNFVCPSDRGND 241

Query: 61  DLLINPFADGSPAIEGLAGEKAVVLVAEKDILRDRGRLYYENLAKSEWKGKVEIFETEGE 120
           DLLINPFADGSP++EGL GEK VV+VAEKDILRDRG LYYENLAKS WKGKV+IFETEGE
Sbjct: 242 DLLINPFADGSPSVEGLVGEKVVVIVAEKDILRDRGMLYYENLAKSHWKGKVDIFETEGE 301

Query: 121 DHAFHILDPTSEKAKSLTKRLALFINED 149
           DHAFH+LDPTSEKAK L  RLALF+NED
Sbjct: 302 DHAFHLLDPTSEKAKCLVNRLALFVNED 329

BLAST of Lag0036935 vs. NCBI nr
Match: XP_022982270.1 (probable carboxylesterase 2 isoform X2 [Cucurbita maxima])

HSP 1 Score: 266.5 bits (680), Expect = 1.3e-67
Identity = 125/148 (84.46%), Postives = 134/148 (90.54%), Query Frame = 0

Query: 1   MALRAKNSDLAGKIKILGIALIQPYFWGQEPIGSEITEHEQKAQVDRWWNFVCPSDGGND 60
           MALRA+NS LAGK KI GIALIQPYFWG+EPIGSEITE E+KA VD WWNFVCPSD GND
Sbjct: 176 MALRAQNSHLAGKFKIRGIALIQPYFWGREPIGSEITEPEKKALVDGWWNFVCPSDRGND 235

Query: 61  DLLINPFADGSPAIEGLAGEKAVVLVAEKDILRDRGRLYYENLAKSEWKGKVEIFETEGE 120
           DLLINPFADGSP++EGL GEK VV+VAEKDILRDRG LYYENLAKS WKGKV+IFETEGE
Sbjct: 236 DLLINPFADGSPSVEGLVGEKVVVIVAEKDILRDRGMLYYENLAKSHWKGKVDIFETEGE 295

Query: 121 DHAFHILDPTSEKAKSLTKRLALFINED 149
           DHAFH+LDPTSEKAK L  RLALF+NED
Sbjct: 296 DHAFHLLDPTSEKAKCLVNRLALFVNED 323

BLAST of Lag0036935 vs. NCBI nr
Match: XP_022940902.1 (probable carboxylesterase 2 [Cucurbita moschata])

HSP 1 Score: 264.6 bits (675), Expect = 5.1e-67
Identity = 123/148 (83.11%), Postives = 133/148 (89.86%), Query Frame = 0

Query: 1   MALRAKNSDLAGKIKILGIALIQPYFWGQEPIGSEITEHEQKAQVDRWWNFVCPSDGGND 60
           MALRA+NS+LAG  KI GIALIQPYFWG+EPIGSEITE E+KA VD WWNFVCPSD GND
Sbjct: 182 MALRAQNSNLAGNFKIRGIALIQPYFWGEEPIGSEITEPEKKALVDSWWNFVCPSDRGND 241

Query: 61  DLLINPFADGSPAIEGLAGEKAVVLVAEKDILRDRGRLYYENLAKSEWKGKVEIFETEGE 120
           DLLINPFADGSP++EGL GEK VV+VAEKDILRDRG LYYENL KS WKGKV+IFETEGE
Sbjct: 242 DLLINPFADGSPSVEGLVGEKVVVIVAEKDILRDRGMLYYENLVKSHWKGKVDIFETEGE 301

Query: 121 DHAFHILDPTSEKAKSLTKRLALFINED 149
           DHAFH+LDPTSEKAK L  RLALF+NED
Sbjct: 302 DHAFHLLDPTSEKAKRLVNRLALFVNED 329

BLAST of Lag0036935 vs. ExPASy Swiss-Prot
Match: Q9SX78 (Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 1.8e-46
Identity = 85/147 (57.82%), Postives = 108/147 (73.47%), Query Frame = 0

Query: 1   MALRAKNSDLAGKIKILGIALIQPYFWGQEPIGSEITEHEQKAQVDRWWNFVCPSDGGND 60
           +A RAK SD   KIK  GI +I PYFWG +PIG+EI +  +K  VD WW FVCPS+ G+D
Sbjct: 167 LAFRAKQSDQTLKIK--GIGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSD 226

Query: 61  DLLINPFADGSPAIEGLAGEKAVVLVAEKDILRDRGRLYYENLAKSEWKGKVEIFETEGE 120
           D  INPFADGSP + GL  E+ ++ VAEKDIL +RG++YYE L KSEWKGKVEI ET+ +
Sbjct: 227 DPWINPFADGSPDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEK 286

Query: 121 DHAFHILDPTSEKAKSLTKRLALFINE 148
           DH FHI +P  ++A  + + LALFIN+
Sbjct: 287 DHVFHIFEPDCDEAMEMVRCLALFINQ 311

BLAST of Lag0036935 vs. ExPASy Swiss-Prot
Match: Q5NUF3 (2-hydroxyisoflavanone dehydratase OS=Glycine max OX=3847 GN=HIDH PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 3.3e-29
Identity = 61/146 (41.78%), Postives = 91/146 (62.33%), Query Frame = 0

Query: 1   MALRAKNSDLAGKIKILGIALIQPYFWGQEPIGSEITEHEQKAQVDRWWNFVCP-SDGGN 60
           + LRA N  L G +KILG  L  P+FWG +PIGSE  E  +++   + WNF CP + GG 
Sbjct: 173 LLLRAGNESLPGDLKILGGLLCCPFFWGSKPIGSEAVEGHEQSLAMKVWNFACPDAPGGI 232

Query: 61  DDLLINPFADGSPAIEGLAGEKAVVLVAEKDILRDRGRLYYENLAKSEWKGKVEIFETEG 120
           D+  INP   G+P++  LA  K +V +  KD  RDR  LY+  + +S W+G++++F+   
Sbjct: 233 DNPWINPCVPGAPSLATLACSKLLVTITGKDEFRDRDILYHHTVEQSGWQGELQLFDAGD 292

Query: 121 EDHAFHILDPTSEKAKSLTKRLALFI 146
           E+HAF +  P +  AK++ KRLA F+
Sbjct: 293 EEHAFQLFKPETHLAKAMIKRLASFL 318

BLAST of Lag0036935 vs. ExPASy Swiss-Prot
Match: Q5NUF4 (2-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 8.2e-28
Identity = 65/145 (44.83%), Postives = 87/145 (60.00%), Query Frame = 0

Query: 2   ALRAKNSDLAGKIKILGIALIQPYFWGQEPIGSEITEHEQKAQVDRWWNFVCP-SDGGND 61
           ALR     L G ++I G+    P FWG +P+ SE  E  +K+   + WNFV P + GG D
Sbjct: 183 ALRVGAEALPGGLRIAGVLSAFPLFWGSKPVLSEPVEGHEKSSPMQVWNFVYPDAPGGID 242

Query: 62  DLLINPFADGSPAIEGLAGEKAVVLVAEKDILRDRGRLYYENLAKSEWKGKVEIFETEGE 121
           + LINP A G+P +  L   K +V VA KD LRDRG  YYE + +S WKG VE+ + EGE
Sbjct: 243 NPLINPLAPGAPNLATLGCPKMLVFVAGKDDLRDRGIWYYEAVKESGWKGDVELAQYEGE 302

Query: 122 DHAFHILDPTSEKAKSLTKRLALFI 146
           +H F I  P +E +K L  R+A F+
Sbjct: 303 EHCFQIYHPETENSKDLIGRIASFL 327

BLAST of Lag0036935 vs. ExPASy Swiss-Prot
Match: I4DST8 (Tuliposide A-converting enzyme 1, chloroplastic OS=Tulipa gesneriana OX=13306 GN=TCEA1 PE=1 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 4.5e-26
Identity = 54/130 (41.54%), Postives = 80/130 (61.54%), Query Frame = 0

Query: 16  ILGIALIQPYFWGQEPIGSEITEHEQKAQVDRWWNFVCPSDGGNDDLLINPFADGSPAIE 75
           I G+A++ PYF G EP+G+EI +       D+ W    P   G DD LINP A G+P++ 
Sbjct: 253 IEGVAIVHPYFLGSEPVGNEINDPANIEFHDKLWRLAAPDTEGLDDPLINPVAPGAPSLA 312

Query: 76  GLAGEKAVVLVAEKDILRDRGRLYYENLAKSEWKGKVEIFETEGEDHAFHILDPTSEKAK 135
           GL  ++AVV VA  D L +RGR+YYE L KS W+G+ E+ + EG  H FH+ D + + + 
Sbjct: 313 GLKCKRAVVFVAGNDFLVERGRMYYEALVKSGWRGEAELVQHEGVGHVFHLSDYSGDISV 372

Query: 136 SLTKRLALFI 146
           ++  +L  F+
Sbjct: 373 AMMTKLIAFL 382

BLAST of Lag0036935 vs. ExPASy Swiss-Prot
Match: I4DST9 (Tuliposide A-converting enzyme 2, chloroplastic OS=Tulipa gesneriana OX=13306 GN=TCEA2 PE=1 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.2e-25
Identity = 54/130 (41.54%), Postives = 78/130 (60.00%), Query Frame = 0

Query: 16  ILGIALIQPYFWGQEPIGSEITEHEQKAQVDRWWNFVCPSDGGNDDLLINPFADGSPAIE 75
           I G+A++ PYF G EP+G+EI +       D+ W    P   G DD LINP A G+P + 
Sbjct: 250 IEGVAIVHPYFLGSEPVGNEINDPANIEFHDKLWRLAAPDTEGLDDPLINPVAPGAPILA 309

Query: 76  GLAGEKAVVLVAEKDILRDRGRLYYENLAKSEWKGKVEIFETEGEDHAFHILDPTSEKAK 135
           GL  ++AVV VA  D L +RGR+YYE L KS W G+ E+ + EG  H FH+ D + + + 
Sbjct: 310 GLKCKRAVVFVAGNDFLVERGRMYYEALVKSGWGGEAELVQHEGVGHVFHLSDYSGDISV 369

Query: 136 SLTKRLALFI 146
           ++  +L  F+
Sbjct: 370 AMMTKLIAFL 379