Lag0036913 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0036913
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionThylakoidal processing peptidase 1
Locationchr2: 2067518 .. 2069773 (+)
RNA-Seq ExpressionLag0036913
SyntenyLag0036913
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTATCAGGGTTACCGTCTCTTTCTCGGGTTATGTTGCCCAAAACCTTGCCGCCTCCGCTGGAATACGTGTCGGTAATTGTCGTGCCGTTCATGAATGCTGGGTTCGTTCTCGAATTTTTGGCTCGAATCAGAAGCCGGAATTTGACCCTACCGGTTCGGTGCGCAATTACCATTCCGACGTTCGTCCGTCTAATTCGAGATGTTGGGTTGAGAATTCAGCATCTTCGTTTGGCACTCTCGCCGGAGAAATTGTTGGTGATAGCTGTAGAAGCCCCCTTGTTCTAGGTTTGATCTCGATTATGAAGTCAACTGCAGTTACTTCCGTTTCCTCGCCAATGGCCATGGGCGTATTTGGCGTTTCTTCCTTCAATGCCGCTTCGATCATCCCATTTTTACAGGGCTCGAAGTGGCTTCCCTGTAACGAATCGATTCCAAGCTCAGCAGGCGGTGAAATAGAAAGTTATGGAGTTTTTGACTGCGTCGTGGACGAGGGCATGAGCAAGCCGCCCAATCCTCCGCGGCTAGAGAAGAGCAGCTGGATATCGCGCTTCTTGAATAACTGTTCTCAGGATGCGAAGGCCATCTTTACAGCTTTGACTGTCAGTGTGCTTTTTCGCTCCTTCTTGGCTGAGCCAAGGTCTATACCTTCTTCATCAATGTATCCAACCCTTGATGTGGGCGACCGCATCTTGGCTGAAAAGGTCAGACAATGTTCTTCTACTGAAATTTTAGTGTTGATTATCTGATAGAACTAGAAGAATGTTCGTTATGAGTTATGGCTATTAATTGGTAATTAGTATGTTTGAGTTGATATGGAAATGTTCAAATTAGTATGCAGATTTAGTTTCCTTCATCAAATGCTCTACTAGAAAGAATTGTTTGGATGCTCTGTTTTCTTATATTTTGTGGGATGTGTGTGTCTAATGTAAAAATGGTTGTCAAGAGGCATGAATTTTACCTATTATTTTCTTAGAATGAAGGGAGCTTGTTTTAATTGCCATATGTTGAGTAACCATGGAGAAATGTGGTTGGAATTTCTGCTGGACAGTTTTTAGAACGATGGTGTACATTGTGAGACTAAGTTTACTTTCTTTTAACCCTGCTTCCTTAAAGACTTGAATTTTCCTTTTCAGTTGTCAAATGACATATAGAGAGAGGATAACCGAAGGAGGTGTTTGATGATTGTTGAAGGGAGAAGAGTAATCTGATAAATAACTGGTTCAACCTTATTGATTACTTGTTTCTTCGTTAACTTGGTCATTCTTCTTATGCTTTTCAGGTGTCATATTTTTCAGAAAGCCGAGTGTTTCTGATATTGTCATATTTAAGGCACCTCCAATCTTGCAGGTAGCTTCTAAGATCTCTATTTTTTATGAAACTTTTCCCTCCCAAATTTTAAACAAGTGGCTGGTCTCTCATTGACAATTCTGGTACATTTGCAGGAAATTGGTTACAAATCGAGTGATGTATTCATCAAGAGAATTGTGGCAAAGGCTGGCGATTGTGTTGAGGTTAGCAATTTTCATCTGATTTAACGATTTCACCGGGTTTGAATCTTTGATCTTACTGACCCTTTTGAAAAATCAATTAACGTTGGAAGTTTCTGAGTCCTAAGCTCACTTTGTTTTATCGATACAGGTTCGGGATGGAAAACTCTTGGTGAATGGTGTTGCTCAAAATGAGAAGAAGTTTATCTTGGAGCCACTTTCTTATGACATGGATCCAGTGGTAATATATCTCAAATCCTTTACTGATTAAACACACCTTTATTTATATATATATATTTTTTTGAAACTCACCTTTATTTATATTTTGTAGAGCATGTCCACTCAATTTATTGTATGTCATGTGTTGTCTTGCAGCTTGTACCTGAAGGGTATGTTTTTGTAATGGGAGACAATCGCAACAACAGTTTTGATTCTCATAACTGGTAATTTGACTATCTTGGATTCAATTTGCTCTTTTCTCAACCGAACATGTTGTCCTTTATAATCAGGCACAGCAGCTCCTCGTTTGTTCTCATCGGGTCTGTTGTCGAATTTCCTTAGATATTGGATCAAGTCTTTAGATAATAATAATCACAATTAACAAATCATTGAAACGTTTCTGAACTGTTCTTGTTTGGTTTTTCCAGGGGTCCACTTCCTGTCGAAAACATTGTTGGTAGATCGGTATTCCGATACTGGCCACCGTCGAAAGTTTCTGATACCATGAGTGACCAAAATGCAGGCAAAGATGTGGTAGTTTCTTGA

mRNA sequence

ATGGCTATCAGGGTTACCGTCTCTTTCTCGGGTTATGTTGCCCAAAACCTTGCCGCCTCCGCTGGAATACGTGTCGGTAATTGTCGTGCCGTTCATGAATGCTGGGTTCGTTCTCGAATTTTTGGCTCGAATCAGAAGCCGGAATTTGACCCTACCGGTTCGGTGCGCAATTACCATTCCGACGTTCGTCCGTCTAATTCGAGATGTTGGGTTGAGAATTCAGCATCTTCGTTTGGCACTCTCGCCGGAGAAATTGTTGGTGATAGCTGTAGAAGCCCCCTTGTTCTAGGTTTGATCTCGATTATGAAGTCAACTGCAGTTACTTCCGTTTCCTCGCCAATGGCCATGGGCGTATTTGGCGTTTCTTCCTTCAATGCCGCTTCGATCATCCCATTTTTACAGGGCTCGAAGTGGCTTCCCTGTAACGAATCGATTCCAAGCTCAGCAGGCGGTGAAATAGAAAGTTATGGAGTTTTTGACTGCGTCGTGGACGAGGGCATGAGCAAGCCGCCCAATCCTCCGCGGCTAGAGAAGAGCAGCTGGATATCGCGCTTCTTGAATAACTGTTCTCAGGATGCGAAGGCCATCTTTACAGCTTTGACTGTCAGTGTGCTTTTTCGCTCCTTCTTGGCTGAGCCAAGGTCTATACCTTCTTCATCAATGTATCCAACCCTTGATGTGGGCGACCGCATCTTGGCTGAAAAGGAAATTGGTTACAAATCGAGTGATGTATTCATCAAGAGAATTGTGGCAAAGGCTGGCGATTGTGTTGAGGTTCGGGATGGAAAACTCTTGGTGAATGGTGTTGCTCAAAATGAGAAGAAGTTTATCTTGGAGCCACTTTCTTATGACATGGATCCAGTGCTTGTACCTGAAGGGTATGTTTTTGTAATGGGAGACAATCGCAACAACAGTTTTGATTCTCATAACTGGGGTCCACTTCCTGTCGAAAACATTGTTGGTAGATCGGTATTCCGATACTGGCCACCGTCGAAAGTTTCTGATACCATGAGTGACCAAAATGCAGGCAAAGATGTGGTAGTTTCTTGA

Coding sequence (CDS)

ATGGCTATCAGGGTTACCGTCTCTTTCTCGGGTTATGTTGCCCAAAACCTTGCCGCCTCCGCTGGAATACGTGTCGGTAATTGTCGTGCCGTTCATGAATGCTGGGTTCGTTCTCGAATTTTTGGCTCGAATCAGAAGCCGGAATTTGACCCTACCGGTTCGGTGCGCAATTACCATTCCGACGTTCGTCCGTCTAATTCGAGATGTTGGGTTGAGAATTCAGCATCTTCGTTTGGCACTCTCGCCGGAGAAATTGTTGGTGATAGCTGTAGAAGCCCCCTTGTTCTAGGTTTGATCTCGATTATGAAGTCAACTGCAGTTACTTCCGTTTCCTCGCCAATGGCCATGGGCGTATTTGGCGTTTCTTCCTTCAATGCCGCTTCGATCATCCCATTTTTACAGGGCTCGAAGTGGCTTCCCTGTAACGAATCGATTCCAAGCTCAGCAGGCGGTGAAATAGAAAGTTATGGAGTTTTTGACTGCGTCGTGGACGAGGGCATGAGCAAGCCGCCCAATCCTCCGCGGCTAGAGAAGAGCAGCTGGATATCGCGCTTCTTGAATAACTGTTCTCAGGATGCGAAGGCCATCTTTACAGCTTTGACTGTCAGTGTGCTTTTTCGCTCCTTCTTGGCTGAGCCAAGGTCTATACCTTCTTCATCAATGTATCCAACCCTTGATGTGGGCGACCGCATCTTGGCTGAAAAGGAAATTGGTTACAAATCGAGTGATGTATTCATCAAGAGAATTGTGGCAAAGGCTGGCGATTGTGTTGAGGTTCGGGATGGAAAACTCTTGGTGAATGGTGTTGCTCAAAATGAGAAGAAGTTTATCTTGGAGCCACTTTCTTATGACATGGATCCAGTGCTTGTACCTGAAGGGTATGTTTTTGTAATGGGAGACAATCGCAACAACAGTTTTGATTCTCATAACTGGGGTCCACTTCCTGTCGAAAACATTGTTGGTAGATCGGTATTCCGATACTGGCCACCGTCGAAAGTTTCTGATACCATGAGTGACCAAAATGCAGGCAAAGATGTGGTAGTTTCTTGA

Protein sequence

MAIRVTVSFSGYVAQNLAASAGIRVGNCRAVHECWVRSRIFGSNQKPEFDPTGSVRNYHSDVRPSNSRCWVENSASSFGTLAGEIVGDSCRSPLVLGLISIMKSTAVTSVSSPMAMGVFGVSSFNAASIIPFLQGSKWLPCNESIPSSAGGEIESYGVFDCVVDEGMSKPPNPPRLEKSSWISRFLNNCSQDAKAIFTALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEKEIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKKFILEPLSYDMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDTMSDQNAGKDVVVS
Homology
BLAST of Lag0036913 vs. NCBI nr
Match: XP_023536434.1 (thylakoidal processing peptidase 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 626.3 bits (1614), Expect = 1.6e-175
Identity = 315/372 (84.68%), Postives = 333/372 (89.52%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLAASAGIRVGNCRAVHECWVRSRIFGSNQKPEFDPTGSVRNYHS 60
           MAIRVTVSFS YVAQNLA SA IRVGNCRAVHECW+RSRIFGSNQKPEFDP+GSVRNY S
Sbjct: 1   MAIRVTVSFSSYVAQNLATSASIRVGNCRAVHECWLRSRIFGSNQKPEFDPSGSVRNYRS 60

Query: 61  DVRPSNSRCWVENSASSFGTLAGEIVGDSCRSPLVLGLISIMKSTAVTSVSSPMAMGVFG 120
           DV PSNS+CWV+NSAS+FGT+AGEIVG+SC+SP+VLGLISIMKST  TS SSPMAMGVFG
Sbjct: 61  DVPPSNSKCWVKNSASAFGTIAGEIVGESCKSPIVLGLISIMKSTVGTSASSPMAMGVFG 120

Query: 121 VSSFNAASIIPFLQGSKWLPCNESIPSSAGGEIESYGVFDCVVDEGMSKPPNPPRLEKSS 180
           VSSF AASIIPFLQGSKWLPCNESIP+ A GE+ESYGVFD V+DEG+S+PPNPPRLEKSS
Sbjct: 121 VSSFRAASIIPFLQGSKWLPCNESIPALASGELESYGVFDSVMDEGISQPPNPPRLEKSS 180

Query: 181 WISRFLNNCSQDAKAIFTALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK----- 240
           WISRFLNNCS+DAKAI TA TVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK     
Sbjct: 181 WISRFLNNCSEDAKAIATAFTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240

Query: 241 ------------------EIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKKFI 300
                             EIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNE KFI
Sbjct: 241 RKPSVSDVVIFKAPPILQEIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNE-KFI 300

Query: 301 LEPLSYDMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDTM 350
           LEPLSY+MDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDT 
Sbjct: 301 LEPLSYNMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDTA 360

BLAST of Lag0036913 vs. NCBI nr
Match: KAG6591353.1 (putative thylakoidal processing peptidase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 624.8 bits (1610), Expect = 4.5e-175
Identity = 314/372 (84.41%), Postives = 331/372 (88.98%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLAASAGIRVGNCRAVHECWVRSRIFGSNQKPEFDPTGSVRNYHS 60
           MAIRVTVSFS YVAQNLA SA IRVGNCRAVHECW+RSRIFGSNQKPEFDP+GSVRNY S
Sbjct: 1   MAIRVTVSFSSYVAQNLATSASIRVGNCRAVHECWLRSRIFGSNQKPEFDPSGSVRNYRS 60

Query: 61  DVRPSNSRCWVENSASSFGTLAGEIVGDSCRSPLVLGLISIMKSTAVTSVSSPMAMGVFG 120
           DV PSNS+CWV+NSAS+FGT+AGEIVG+SC+SP+VLGLISIMKST  TS SSPM MGVFG
Sbjct: 61  DVPPSNSKCWVKNSASAFGTIAGEIVGESCKSPIVLGLISIMKSTVGTSASSPMTMGVFG 120

Query: 121 VSSFNAASIIPFLQGSKWLPCNESIPSSAGGEIESYGVFDCVVDEGMSKPPNPPRLEKSS 180
           VSSF AASIIPFLQGSKWLPCNESIP+ A GE+ESYGVFD  +DEGMS+PPNPPRLEKSS
Sbjct: 121 VSSFRAASIIPFLQGSKWLPCNESIPALASGELESYGVFDSAMDEGMSQPPNPPRLEKSS 180

Query: 181 WISRFLNNCSQDAKAIFTALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK----- 240
           WISRFLNNCS+DAKAI TA TVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK     
Sbjct: 181 WISRFLNNCSEDAKAIATAFTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240

Query: 241 ------------------EIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKKFI 300
                             EIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNE KFI
Sbjct: 241 RKPSVSDVVIFKAPPILQEIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNE-KFI 300

Query: 301 LEPLSYDMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDTM 350
           LEPLSY+MDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDT 
Sbjct: 301 LEPLSYNMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDTA 360

BLAST of Lag0036913 vs. NCBI nr
Match: KAG7024230.1 (putative thylakoidal processing peptidase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 624.0 bits (1608), Expect = 7.7e-175
Identity = 315/378 (83.33%), Postives = 332/378 (87.83%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLAASAGIRVGNCRAVHECWVRSRIFGSNQKPEFDPTGSVRNYHS 60
           MAIRVTVSFS YVAQNLA SA IRVGNCRAVHECW+RSRIFGSNQKPEFDP+GSVRNY S
Sbjct: 1   MAIRVTVSFSSYVAQNLATSASIRVGNCRAVHECWLRSRIFGSNQKPEFDPSGSVRNYRS 60

Query: 61  DVRPSNSRCWVENSASSFGTLAGEIVGDSCRSPLVLGLISIMKSTAVTSVSSPMAMGVFG 120
           DV PSNS+CWV+NSAS+FGT+AGEIVG+SC+SP+VLGLISIMKST  TS SSPMAMGVFG
Sbjct: 61  DVPPSNSKCWVKNSASAFGTIAGEIVGESCKSPIVLGLISIMKSTVGTSASSPMAMGVFG 120

Query: 121 VSSFNAASIIPFLQGSKWLPCNESIPSSAGGEIESYGVFDCVVDEGMSKPPNPPRLEKSS 180
           VSSF AASIIPFLQGSKWLPCNESIP+ A GE+ESYGVFD  +DEGMS+PPNPPRLEKSS
Sbjct: 121 VSSFRAASIIPFLQGSKWLPCNESIPALASGELESYGVFDSAMDEGMSQPPNPPRLEKSS 180

Query: 181 WISRFLNNCSQDAKAIFTALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK----- 240
           WISRFLNNCS+DAKAI TA TVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK     
Sbjct: 181 WISRFLNNCSEDAKAIATAFTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEKSVFAF 240

Query: 241 ------------------------EIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQ 300
                                   EIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQ
Sbjct: 241 QVSYFFRKPSVSDVVIFKAPPILQEIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQ 300

Query: 301 NEKKFILEPLSYDMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPS 350
           NE KFILEPLSY+MDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPS
Sbjct: 301 NE-KFILEPLSYNMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPS 360

BLAST of Lag0036913 vs. NCBI nr
Match: XP_022976232.1 (thylakoidal processing peptidase 1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 624.0 bits (1608), Expect = 7.7e-175
Identity = 314/372 (84.41%), Postives = 332/372 (89.25%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLAASAGIRVGNCRAVHECWVRSRIFGSNQKPEFDPTGSVRNYHS 60
           MAIRVTVSFS YVAQNLA SA IRVG+CRAVHECW+RSRIFGSNQKPEFDP+GSVRNY S
Sbjct: 1   MAIRVTVSFSSYVAQNLATSASIRVGSCRAVHECWLRSRIFGSNQKPEFDPSGSVRNYRS 60

Query: 61  DVRPSNSRCWVENSASSFGTLAGEIVGDSCRSPLVLGLISIMKSTAVTSVSSPMAMGVFG 120
           DV PSNS+CWV+NSAS+FGT+AGEIVG+SC+SP+VLGLISIMKSTA TS SSPMAMGVFG
Sbjct: 61  DVPPSNSKCWVKNSASAFGTIAGEIVGESCKSPIVLGLISIMKSTAGTSASSPMAMGVFG 120

Query: 121 VSSFNAASIIPFLQGSKWLPCNESIPSSAGGEIESYGVFDCVVDEGMSKPPNPPRLEKSS 180
           VSSF AASIIPFLQGSKWLPCNESIP+ A GE+ESYGVFD  +DEGMS+PPNPPRLEKSS
Sbjct: 121 VSSFRAASIIPFLQGSKWLPCNESIPALASGELESYGVFDSAMDEGMSQPPNPPRLEKSS 180

Query: 181 WISRFLNNCSQDAKAIFTALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK----- 240
           WISRFLNNCS+DAKAI TA TVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK     
Sbjct: 181 WISRFLNNCSEDAKAIATAFTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240

Query: 241 ------------------EIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKKFI 300
                             EIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNE KFI
Sbjct: 241 RKPSVSDVVIFKAPPILQEIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNE-KFI 300

Query: 301 LEPLSYDMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDTM 350
           LEPLSY+MDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDT 
Sbjct: 301 LEPLSYNMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDTA 360

BLAST of Lag0036913 vs. NCBI nr
Match: XP_038900232.1 (thylakoidal processing peptidase 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 624.0 bits (1608), Expect = 7.7e-175
Identity = 317/372 (85.22%), Postives = 333/372 (89.52%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLAASAGIRVGNCRAVHECWVRSRIFGSNQKPEFDPTGSVRNYHS 60
           MAIRVTVSFSGYVAQNLA SAGIRVGNCRAVHECW+RSR FGSNQKPEFD  GSVR+YHS
Sbjct: 1   MAIRVTVSFSGYVAQNLATSAGIRVGNCRAVHECWIRSRFFGSNQKPEFDHPGSVRHYHS 60

Query: 61  DVRPSNSRCWVENSASSFGTLAGEIVGDSCRSPLVLGLISIMKSTAVTSVSSPMAMGVFG 120
           DV PSNSRCWV+NSASSFGT+AGEIVG+S R+P+VLGLISIMKST VTSVSSPMAMGVFG
Sbjct: 61  DVLPSNSRCWVKNSASSFGTIAGEIVGESFRNPIVLGLISIMKSTVVTSVSSPMAMGVFG 120

Query: 121 VSSFNAASIIPFLQGSKWLPCNESIPSSAGGEIESYGVFDCVVDEGMSKPPNPPRLEKSS 180
           VSSFNAAS+IPFLQGSK +  N+S+PSSAGGEIESYGVFDCV+DEGMS PPNPP+LEKSS
Sbjct: 121 VSSFNAASVIPFLQGSKSVTGNDSVPSSAGGEIESYGVFDCVMDEGMSLPPNPPQLEKSS 180

Query: 181 WISRFLNNCSQDAKAIFTALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK----- 240
           WISRFLNNCSQDAKAI TALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK     
Sbjct: 181 WISRFLNNCSQDAKAIVTALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240

Query: 241 ------------------EIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKKFI 300
                             EIGYKS+DVFIKRIVAKAGDCVEVRDGKLLVNGVAQNE KFI
Sbjct: 241 RKPSVSDIVIFKAPPILQEIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNE-KFI 300

Query: 301 LEPLSYDMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDTM 350
           LEPLSY+MDPVLVPEGYVFV+GDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSD M
Sbjct: 301 LEPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDIM 360

BLAST of Lag0036913 vs. ExPASy Swiss-Prot
Match: Q9M9Z2 (Probable thylakoidal processing peptidase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TPP2 PE=2 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 7.5e-88
Identity = 185/377 (49.07%), Postives = 236/377 (62.60%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLAASAGIRV--GNCRAVHECWVRSRIFGSNQKPEFDPTGSVRNY 60
           MAIRVT ++S YVA+++A+SAG RV  G+ R+  E WVR R  G NQ P+        N 
Sbjct: 1   MAIRVTFTYSSYVARSIASSAGTRVGTGDVRSCFETWVRPRFCGHNQIPDIVDKSPGSNT 60

Query: 61  HSDVRPSNSRCWVENSASSFGTLAGEIVGDSCRSPLVLGLISIMKSTAVTSVSSPMAMGV 120
                   +R     ++S + T+A EI+ + C+SPLVLG+IS+M  T     S    M  
Sbjct: 61  WGPSSGPRAR----PASSMYSTIAREILEEGCKSPLVLGMISLMNLTGAPQFS---GMTG 120

Query: 121 FGVSSFNAASIIPFLQGSKWLPCNESIPSSAGGEIESYGVFDCVVDEGMSKPPNPPRLEK 180
            G+S F  +S+IPFL+GSKW+PC  SIP++   +I         VD G        +LE 
Sbjct: 121 LGISPFKTSSVIPFLRGSKWMPC--SIPATLSTDIAE-------VDRGGKVCDPKVKLEL 180

Query: 181 S--------SWISRFLNNCSQDAKAIFTALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDR 240
           S         W+++ LN CS+DAKA FTA+TVS+LFRS LAEP+SIPS+SM PTLDVGDR
Sbjct: 181 SDKVSNGGNGWVNKLLNICSEDAKAAFTAVTVSLLFRSALAEPKSIPSTSMLPTLDVGDR 240

Query: 241 ILAEK-----------------------EIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVN 300
           ++AEK                       E GY  +DVFIKRIVA  GD VEV DGKLLVN
Sbjct: 241 VIAEKVSYFFRKPEVSDIVIFKAPPILVEHGYSCADVFIKRIVASEGDWVEVCDGKLLVN 300

Query: 301 GVAQNEKKFILEPLSYDMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRY 345
              Q E  F+LEP+ Y+M+P+ VPEGYVFV+GDNRN SFDSHNWGPLP++NI+GRSVFRY
Sbjct: 301 DTVQAE-DFVLEPIDYEMEPMFVPEGYVFVLGDNRNKSFDSHNWGPLPIKNIIGRSVFRY 360

BLAST of Lag0036913 vs. ExPASy Swiss-Prot
Match: O04348 (Thylakoidal processing peptidase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TPP1 PE=2 SV=2)

HSP 1 Score: 316.6 bits (810), Expect = 3.5e-85
Identity = 190/368 (51.63%), Postives = 236/368 (64.13%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLAASAGIRVGNCRAVHECWVRSRIFGSNQKPEFDPTGSVRNYHS 60
           MAIR+T ++S +VA+NL    G RVG      E  VR R F  + K +FD   S RN   
Sbjct: 1   MAIRITFTYSTHVARNL---VGTRVGPGGYCFESLVRPRFF--SHKRDFD--RSPRN--- 60

Query: 61  DVRPSNSRCWVENSASSFGTLAGEIVGDSCRSPLVLGLISIMKSTAVTSVSSPMAMGVFG 120
             RP          AS +G++A E++G+  +SPLV+GLISI+KST     S+   M V G
Sbjct: 61  --RP----------ASMYGSIARELIGEGSQSPLVMGLISILKSTTGHESST---MNVLG 120

Query: 121 VSSFNAASIIPFLQGSKWLPCNESIPSSAGGEIESYGVFDCVVDEGMSKPPNPPRLEKSS 180
           VSSF A+SIIPFLQGSKW+     I      +++  G    V D+   K     R   S 
Sbjct: 121 VSSFKASSIIPFLQGSKWIKNPPVI-----DDVDKGGT---VCDDDDDK---ESRNGGSG 180

Query: 181 WISRFLNNCSQDAKAIFTALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK----- 240
           W+++ L+ CS+DAKA FTA+TVS+LFRS LAEP+SIPS+SMYPTLD GDR++AEK     
Sbjct: 181 WVNKLLSVCSEDAKAAFTAVTVSILFRSALAEPKSIPSTSMYPTLDKGDRVMAEKVSYFF 240

Query: 241 ---------------------EIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEK 300
                                E GY S+DVFIKRIVA  GD VEVRDGKL VN + Q E+
Sbjct: 241 RKPEVSDIVIFKAPPILLEYPEYGYSSNDVFIKRIVASEGDWVEVRDGKLFVNDIVQ-EE 300

Query: 301 KFILEPLSYDMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVS 343
            F+LEP+SY+M+P+ VP+GYVFV+GDNRN SFDSHNWGPLP+ENIVGRSVFRYWPPSKVS
Sbjct: 301 DFVLEPMSYEMEPMFVPKGYVFVLGDNRNKSFDSHNWGPLPIENIVGRSVFRYWPPSKVS 331

BLAST of Lag0036913 vs. ExPASy Swiss-Prot
Match: Q8H0W1 (Chloroplast processing peptidase OS=Arabidopsis thaliana OX=3702 GN=PLSP1 PE=2 SV=2)

HSP 1 Score: 214.9 bits (546), Expect = 1.4e-54
Identity = 108/192 (56.25%), Postives = 133/192 (69.27%), Query Frame = 0

Query: 177 EKSSWISRFLNNCSQDAKAIFTALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK- 236
           EK+     +L+  S DA+ +F A+ VS+ FR F+AEPR IPS SMYPT DVGDR++AEK 
Sbjct: 99  EKNRLFPEWLDFTSDDAQTVFVAIAVSLAFRYFIAEPRYIPSLSMYPTFDVGDRLVAEKV 158

Query: 237 ----------------------EIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNE 296
                                 E+GY  +DVFIKRIVAK GD VEV +GKL+VNGVA+NE
Sbjct: 159 SYYFRKPCANDIVIFKSPPVLQEVGYTDADVFIKRIVAKEGDLVEVHNGKLMVNGVARNE 218

Query: 297 KKFILEPLSYDMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKV 346
            KFILEP  Y+M P+ VPE  VFVMGDNRNNS+DSH WGPLP++NI+GRSVFRYWPP++V
Sbjct: 219 -KFILEPPGYEMTPIRVPENSVFVMGDNRNNSYDSHVWGPLPLKNIIGRSVFRYWPPNRV 278

BLAST of Lag0036913 vs. ExPASy Swiss-Prot
Match: P72660 (Probable signal peptidase I-1 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=lepB1 PE=3 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 4.6e-29
Identity = 71/160 (44.38%), Postives = 97/160 (60.62%), Query Frame = 0

Query: 196 IFTALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK-------------------E 255
           +  AL +++L R F+AEPR IPS SM PTL+ GDR++ EK                   E
Sbjct: 20  LMVALVLALLLRFFVAEPRYIPSDSMLPTLEQGDRLVVEKVSYHFHPPQVGDIIVFHPPE 79

Query: 256 I----GYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKKFILEPLSYDMDPVLVPE 315
           +    GY     FIKR++A  G  VEV +G +  +G    E ++ILEP  Y++  V VP+
Sbjct: 80  LLQVQGYDLGQAFIKRVIALPGQTVEVNNGIVYRDGQPLQE-EYILEPPQYNLPAVRVPD 139

Query: 316 GYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSK 333
           G VFVMGDNRNNS DSH WG LP +NI+G ++FR++P S+
Sbjct: 140 GQVFVMGDNRNNSNDSHVWGFLPQQNIIGHALFRFFPASR 178

BLAST of Lag0036913 vs. ExPASy Swiss-Prot
Match: P73157 (Probable signal peptidase I-2 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=lepB2 PE=3 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 5.6e-27
Identity = 72/194 (37.11%), Postives = 105/194 (54.12%), Query Frame = 0

Query: 162 VVDEGMSKPPNPPRLEKSSWISRFLNNCSQDAKAIFTALTVSVLFRSFLAEPRSIPSSSM 221
           +V E   K  +PP   +++W+        +  K + TA+ +++  R+F+AE R IPSSSM
Sbjct: 5   IVRETSKKKESPP---ENTWL--------ELGKTMVTAVILAIGIRTFVAEARYIPSSSM 64

Query: 222 YPTLDVGDRILAEKEIGYK----------------------SSDVFIKRIVAKAGDCVEV 281
            PTL + DR++ EK I Y+                        D FIKRI+   GD V V
Sbjct: 65  EPTLQINDRLIIEK-ISYRLRDPERGEIVVFNPTDALKAKNFHDAFIKRIIGLPGDEVRV 124

Query: 282 RDGKLLVNGVAQNEKKFILEPLSYDMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENI 334
             G + VNG   +E  +I  P +Y+  PV VP+    V+GDNRNNS+DSH WG +P E +
Sbjct: 125 SQGNVYVNGKMLDE-NYIAAPPAYEYGPVKVPDDQYLVLGDNRNNSYDSHYWGFVPREKL 184

BLAST of Lag0036913 vs. ExPASy TrEMBL
Match: A0A6J1IF81 (thylakoidal processing peptidase 1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476692 PE=4 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 3.7e-175
Identity = 314/372 (84.41%), Postives = 332/372 (89.25%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLAASAGIRVGNCRAVHECWVRSRIFGSNQKPEFDPTGSVRNYHS 60
           MAIRVTVSFS YVAQNLA SA IRVG+CRAVHECW+RSRIFGSNQKPEFDP+GSVRNY S
Sbjct: 1   MAIRVTVSFSSYVAQNLATSASIRVGSCRAVHECWLRSRIFGSNQKPEFDPSGSVRNYRS 60

Query: 61  DVRPSNSRCWVENSASSFGTLAGEIVGDSCRSPLVLGLISIMKSTAVTSVSSPMAMGVFG 120
           DV PSNS+CWV+NSAS+FGT+AGEIVG+SC+SP+VLGLISIMKSTA TS SSPMAMGVFG
Sbjct: 61  DVPPSNSKCWVKNSASAFGTIAGEIVGESCKSPIVLGLISIMKSTAGTSASSPMAMGVFG 120

Query: 121 VSSFNAASIIPFLQGSKWLPCNESIPSSAGGEIESYGVFDCVVDEGMSKPPNPPRLEKSS 180
           VSSF AASIIPFLQGSKWLPCNESIP+ A GE+ESYGVFD  +DEGMS+PPNPPRLEKSS
Sbjct: 121 VSSFRAASIIPFLQGSKWLPCNESIPALASGELESYGVFDSAMDEGMSQPPNPPRLEKSS 180

Query: 181 WISRFLNNCSQDAKAIFTALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK----- 240
           WISRFLNNCS+DAKAI TA TVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK     
Sbjct: 181 WISRFLNNCSEDAKAIATAFTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240

Query: 241 ------------------EIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKKFI 300
                             EIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNE KFI
Sbjct: 241 RKPSVSDVVIFKAPPILQEIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNE-KFI 300

Query: 301 LEPLSYDMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDTM 350
           LEPLSY+MDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDT 
Sbjct: 301 LEPLSYNMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDTA 360

BLAST of Lag0036913 vs. ExPASy TrEMBL
Match: A0A6J1FC76 (thylakoidal processing peptidase 1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111442773 PE=4 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 3.7e-175
Identity = 314/372 (84.41%), Postives = 331/372 (88.98%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLAASAGIRVGNCRAVHECWVRSRIFGSNQKPEFDPTGSVRNYHS 60
           MAIRVTVSFS YVAQNLA SA IRVGNCRAVHECW+RSRIFGSNQKPEFDP+GSVRNY S
Sbjct: 1   MAIRVTVSFSSYVAQNLATSASIRVGNCRAVHECWLRSRIFGSNQKPEFDPSGSVRNYRS 60

Query: 61  DVRPSNSRCWVENSASSFGTLAGEIVGDSCRSPLVLGLISIMKSTAVTSVSSPMAMGVFG 120
           DV PSNS+CWV+NSAS+FGT+AGEIVG+SC+SP+VLGLISIMKST  TS SSPMAMGVFG
Sbjct: 61  DVPPSNSKCWVKNSASAFGTIAGEIVGESCKSPIVLGLISIMKSTVGTSASSPMAMGVFG 120

Query: 121 VSSFNAASIIPFLQGSKWLPCNESIPSSAGGEIESYGVFDCVVDEGMSKPPNPPRLEKSS 180
           VSSF AASIIPFLQGSKWLPCNESIP+ A GE+ESYGVFD  +DE MS+PPNPPRLEKSS
Sbjct: 121 VSSFRAASIIPFLQGSKWLPCNESIPALASGELESYGVFDSAMDESMSQPPNPPRLEKSS 180

Query: 181 WISRFLNNCSQDAKAIFTALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK----- 240
           WISRFLNNCS+DAKAI TA TVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK     
Sbjct: 181 WISRFLNNCSEDAKAIATAFTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240

Query: 241 ------------------EIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKKFI 300
                             EIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNE KFI
Sbjct: 241 RKPSVSDVVIFKAPPILQEIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNE-KFI 300

Query: 301 LEPLSYDMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDTM 350
           LEPLSY+MDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDT 
Sbjct: 301 LEPLSYNMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDTA 360

BLAST of Lag0036913 vs. ExPASy TrEMBL
Match: A0A6J1CD84 (uncharacterized protein LOC111010591 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010591 PE=4 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 5.6e-171
Identity = 306/369 (82.93%), Postives = 326/369 (88.35%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLAASAGIRVGNCRAVHECWVRSRIFGSNQKPEFDPTGSVRNYHS 60
           MAIRVTVSFSGYVAQNLA+SAG RVGNCRAVHECW+RSRIFGSNQKPEFDP+G+ RNY  
Sbjct: 1   MAIRVTVSFSGYVAQNLASSAGFRVGNCRAVHECWIRSRIFGSNQKPEFDPSGAARNYRP 60

Query: 61  DVRPSNSRCWVENSASSFGTLAGEIVGDSCRSPLVLGLISIMKSTAVTSVSSPMAMGVFG 120
           D+RPSNS+CWV+NSASSF TLAGEIVGD+CRSPL+LGLISIMKSTA TSVSSPMAMG+FG
Sbjct: 61  DIRPSNSKCWVKNSASSFSTLAGEIVGDNCRSPLLLGLISIMKSTACTSVSSPMAMGIFG 120

Query: 121 VSSFNAASIIPFLQGSKWLPCNESIPSSAGGEIESYGVFDCVVDEGMSKPPNPPRLEKSS 180
           VSSFNAASIIPFLQGSKWLPCNESIP SA  EIESYGVFD   DEG+SKPPNPPRLEKSS
Sbjct: 121 VSSFNAASIIPFLQGSKWLPCNESIPHSASAEIESYGVFDSAADEGLSKPPNPPRLEKSS 180

Query: 181 WISRFLNNCSQDAKAIFTALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK----- 240
           W SRFLNNCS+DAKAI TALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK     
Sbjct: 181 WFSRFLNNCSEDAKAIVTALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240

Query: 241 ------------------EIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKKFI 300
                             E+GYKSSDVFIKR+VAKAGD VEVRDGKLLVNG AQ+E +FI
Sbjct: 241 RKPSVSDIVIFKAPPILQEVGYKSSDVFIKRVVAKAGDYVEVRDGKLLVNGDAQDE-EFI 300

Query: 301 LEPLSYDMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDTM 346
           LEPLSYDMDP+LVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDT 
Sbjct: 301 LEPLSYDMDPMLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDTT 360

BLAST of Lag0036913 vs. ExPASy TrEMBL
Match: A0A6J1CEX6 (thylakoidal processing peptidase 1, chloroplastic-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111010591 PE=4 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 5.6e-171
Identity = 306/369 (82.93%), Postives = 326/369 (88.35%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLAASAGIRVGNCRAVHECWVRSRIFGSNQKPEFDPTGSVRNYHS 60
           MAIRVTVSFSGYVAQNLA+SAG RVGNCRAVHECW+RSRIFGSNQKPEFDP+G+ RNY  
Sbjct: 1   MAIRVTVSFSGYVAQNLASSAGFRVGNCRAVHECWIRSRIFGSNQKPEFDPSGAARNYRP 60

Query: 61  DVRPSNSRCWVENSASSFGTLAGEIVGDSCRSPLVLGLISIMKSTAVTSVSSPMAMGVFG 120
           D+RPSNS+CWV+NSASSF TLAGEIVGD+CRSPL+LGLISIMKSTA TSVSSPMAMG+FG
Sbjct: 61  DIRPSNSKCWVKNSASSFSTLAGEIVGDNCRSPLLLGLISIMKSTACTSVSSPMAMGIFG 120

Query: 121 VSSFNAASIIPFLQGSKWLPCNESIPSSAGGEIESYGVFDCVVDEGMSKPPNPPRLEKSS 180
           VSSFNAASIIPFLQGSKWLPCNESIP SA  EIESYGVFD   DEG+SKPPNPPRLEKSS
Sbjct: 121 VSSFNAASIIPFLQGSKWLPCNESIPHSASAEIESYGVFDSAADEGLSKPPNPPRLEKSS 180

Query: 181 WISRFLNNCSQDAKAIFTALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK----- 240
           W SRFLNNCS+DAKAI TALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK     
Sbjct: 181 WFSRFLNNCSEDAKAIVTALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240

Query: 241 ------------------EIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKKFI 300
                             E+GYKSSDVFIKR+VAKAGD VEVRDGKLLVNG AQ+E +FI
Sbjct: 241 RKPSVSDIVIFKAPPILQEVGYKSSDVFIKRVVAKAGDYVEVRDGKLLVNGDAQDE-EFI 300

Query: 301 LEPLSYDMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDTM 346
           LEPLSYDMDP+LVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDT 
Sbjct: 301 LEPLSYDMDPMLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDTT 360

BLAST of Lag0036913 vs. ExPASy TrEMBL
Match: A0A6J1CGI4 (uncharacterized protein LOC111010591 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010591 PE=4 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 5.6e-171
Identity = 306/369 (82.93%), Postives = 326/369 (88.35%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLAASAGIRVGNCRAVHECWVRSRIFGSNQKPEFDPTGSVRNYHS 60
           MAIRVTVSFSGYVAQNLA+SAG RVGNCRAVHECW+RSRIFGSNQKPEFDP+G+ RNY  
Sbjct: 1   MAIRVTVSFSGYVAQNLASSAGFRVGNCRAVHECWIRSRIFGSNQKPEFDPSGAARNYRP 60

Query: 61  DVRPSNSRCWVENSASSFGTLAGEIVGDSCRSPLVLGLISIMKSTAVTSVSSPMAMGVFG 120
           D+RPSNS+CWV+NSASSF TLAGEIVGD+CRSPL+LGLISIMKSTA TSVSSPMAMG+FG
Sbjct: 61  DIRPSNSKCWVKNSASSFSTLAGEIVGDNCRSPLLLGLISIMKSTACTSVSSPMAMGIFG 120

Query: 121 VSSFNAASIIPFLQGSKWLPCNESIPSSAGGEIESYGVFDCVVDEGMSKPPNPPRLEKSS 180
           VSSFNAASIIPFLQGSKWLPCNESIP SA  EIESYGVFD   DEG+SKPPNPPRLEKSS
Sbjct: 121 VSSFNAASIIPFLQGSKWLPCNESIPHSASAEIESYGVFDSAADEGLSKPPNPPRLEKSS 180

Query: 181 WISRFLNNCSQDAKAIFTALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK----- 240
           W SRFLNNCS+DAKAI TALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK     
Sbjct: 181 WFSRFLNNCSEDAKAIVTALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240

Query: 241 ------------------EIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKKFI 300
                             E+GYKSSDVFIKR+VAKAGD VEVRDGKLLVNG AQ+E +FI
Sbjct: 241 RKPSVSDIVIFKAPPILQEVGYKSSDVFIKRVVAKAGDYVEVRDGKLLVNGDAQDE-EFI 300

Query: 301 LEPLSYDMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDTM 346
           LEPLSYDMDP+LVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDT 
Sbjct: 301 LEPLSYDMDPMLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDTT 360

BLAST of Lag0036913 vs. TAIR 10
Match: AT1G06870.1 (Peptidase S24/S26A/S26B/S26C family protein )

HSP 1 Score: 325.5 bits (833), Expect = 5.3e-89
Identity = 185/377 (49.07%), Postives = 236/377 (62.60%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLAASAGIRV--GNCRAVHECWVRSRIFGSNQKPEFDPTGSVRNY 60
           MAIRVT ++S YVA+++A+SAG RV  G+ R+  E WVR R  G NQ P+        N 
Sbjct: 1   MAIRVTFTYSSYVARSIASSAGTRVGTGDVRSCFETWVRPRFCGHNQIPDIVDKSPGSNT 60

Query: 61  HSDVRPSNSRCWVENSASSFGTLAGEIVGDSCRSPLVLGLISIMKSTAVTSVSSPMAMGV 120
                   +R     ++S + T+A EI+ + C+SPLVLG+IS+M  T     S    M  
Sbjct: 61  WGPSSGPRAR----PASSMYSTIAREILEEGCKSPLVLGMISLMNLTGAPQFS---GMTG 120

Query: 121 FGVSSFNAASIIPFLQGSKWLPCNESIPSSAGGEIESYGVFDCVVDEGMSKPPNPPRLEK 180
            G+S F  +S+IPFL+GSKW+PC  SIP++   +I         VD G        +LE 
Sbjct: 121 LGISPFKTSSVIPFLRGSKWMPC--SIPATLSTDIAE-------VDRGGKVCDPKVKLEL 180

Query: 181 S--------SWISRFLNNCSQDAKAIFTALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDR 240
           S         W+++ LN CS+DAKA FTA+TVS+LFRS LAEP+SIPS+SM PTLDVGDR
Sbjct: 181 SDKVSNGGNGWVNKLLNICSEDAKAAFTAVTVSLLFRSALAEPKSIPSTSMLPTLDVGDR 240

Query: 241 ILAEK-----------------------EIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVN 300
           ++AEK                       E GY  +DVFIKRIVA  GD VEV DGKLLVN
Sbjct: 241 VIAEKVSYFFRKPEVSDIVIFKAPPILVEHGYSCADVFIKRIVASEGDWVEVCDGKLLVN 300

Query: 301 GVAQNEKKFILEPLSYDMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRY 345
              Q E  F+LEP+ Y+M+P+ VPEGYVFV+GDNRN SFDSHNWGPLP++NI+GRSVFRY
Sbjct: 301 DTVQAE-DFVLEPIDYEMEPMFVPEGYVFVLGDNRNKSFDSHNWGPLPIKNIIGRSVFRY 360

BLAST of Lag0036913 vs. TAIR 10
Match: AT2G30440.1 (thylakoid processing peptide )

HSP 1 Score: 316.6 bits (810), Expect = 2.5e-86
Identity = 190/368 (51.63%), Postives = 236/368 (64.13%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLAASAGIRVGNCRAVHECWVRSRIFGSNQKPEFDPTGSVRNYHS 60
           MAIR+T ++S +VA+NL    G RVG      E  VR R F  + K +FD   S RN   
Sbjct: 1   MAIRITFTYSTHVARNL---VGTRVGPGGYCFESLVRPRFF--SHKRDFD--RSPRN--- 60

Query: 61  DVRPSNSRCWVENSASSFGTLAGEIVGDSCRSPLVLGLISIMKSTAVTSVSSPMAMGVFG 120
             RP          AS +G++A E++G+  +SPLV+GLISI+KST     S+   M V G
Sbjct: 61  --RP----------ASMYGSIARELIGEGSQSPLVMGLISILKSTTGHESST---MNVLG 120

Query: 121 VSSFNAASIIPFLQGSKWLPCNESIPSSAGGEIESYGVFDCVVDEGMSKPPNPPRLEKSS 180
           VSSF A+SIIPFLQGSKW+     I      +++  G    V D+   K     R   S 
Sbjct: 121 VSSFKASSIIPFLQGSKWIKNPPVI-----DDVDKGGT---VCDDDDDK---ESRNGGSG 180

Query: 181 WISRFLNNCSQDAKAIFTALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK----- 240
           W+++ L+ CS+DAKA FTA+TVS+LFRS LAEP+SIPS+SMYPTLD GDR++AEK     
Sbjct: 181 WVNKLLSVCSEDAKAAFTAVTVSILFRSALAEPKSIPSTSMYPTLDKGDRVMAEKVSYFF 240

Query: 241 ---------------------EIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEK 300
                                E GY S+DVFIKRIVA  GD VEVRDGKL VN + Q E+
Sbjct: 241 RKPEVSDIVIFKAPPILLEYPEYGYSSNDVFIKRIVASEGDWVEVRDGKLFVNDIVQ-EE 300

Query: 301 KFILEPLSYDMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVS 343
            F+LEP+SY+M+P+ VP+GYVFV+GDNRN SFDSHNWGPLP+ENIVGRSVFRYWPPSKVS
Sbjct: 301 DFVLEPMSYEMEPMFVPKGYVFVLGDNRNKSFDSHNWGPLPIENIVGRSVFRYWPPSKVS 331

BLAST of Lag0036913 vs. TAIR 10
Match: AT3G24590.1 (plastidic type i signal peptidase 1 )

HSP 1 Score: 214.9 bits (546), Expect = 1.0e-55
Identity = 108/192 (56.25%), Postives = 133/192 (69.27%), Query Frame = 0

Query: 177 EKSSWISRFLNNCSQDAKAIFTALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEK- 236
           EK+     +L+  S DA+ +F A+ VS+ FR F+AEPR IPS SMYPT DVGDR++AEK 
Sbjct: 99  EKNRLFPEWLDFTSDDAQTVFVAIAVSLAFRYFIAEPRYIPSLSMYPTFDVGDRLVAEKV 158

Query: 237 ----------------------EIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNE 296
                                 E+GY  +DVFIKRIVAK GD VEV +GKL+VNGVA+NE
Sbjct: 159 SYYFRKPCANDIVIFKSPPVLQEVGYTDADVFIKRIVAKEGDLVEVHNGKLMVNGVARNE 218

Query: 297 KKFILEPLSYDMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKV 346
            KFILEP  Y+M P+ VPE  VFVMGDNRNNS+DSH WGPLP++NI+GRSVFRYWPP++V
Sbjct: 219 -KFILEPPGYEMTPIRVPENSVFVMGDNRNNSYDSHVWGPLPLKNIIGRSVFRYWPPNRV 278

BLAST of Lag0036913 vs. TAIR 10
Match: AT1G23465.1 (Peptidase S24/S26A/S26B/S26C family protein )

HSP 1 Score: 56.6 bits (135), Expect = 4.6e-08
Identity = 30/82 (36.59%), Postives = 45/82 (54.88%), Query Frame = 0

Query: 246 IKRIVAKAGDCVEVRDGKLLVNGVAQNEKKFILEPLSYD-MDPVLVPEGYVFVMGDNRNN 305
           IKR+V   GDC+                  F+++P+  D    ++VP+G+VFV GD  +N
Sbjct: 90  IKRVVGVEGDCI-----------------SFVIDPVKSDESQTIVVPKGHVFVQGDYTHN 149

Query: 306 SFDSHNWGPLPVENIVGRSVFR 327
           S DS N+GP+P   I GR ++R
Sbjct: 150 SRDSRNFGPVPYGLIQGRVLWR 154

BLAST of Lag0036913 vs. TAIR 10
Match: AT3G08980.1 (Peptidase S24/S26A/S26B/S26C family protein )

HSP 1 Score: 56.2 bits (134), Expect = 6.0e-08
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 0

Query: 279 EPLSYDMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVS 335
           E +S   D + VPEG+ +V GDN+ +S DS ++GP+P+  I GR     WPP ++S
Sbjct: 95  EWISSSRDVIRVPEGHCWVEGDNKTSSLDSRSFGPIPLGLIQGRVTRVMWPPQRIS 150

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023536434.11.6e-17584.68thylakoidal processing peptidase 1, chloroplastic-like [Cucurbita pepo subsp. pe... [more]
KAG6591353.14.5e-17584.41putative thylakoidal processing peptidase 2, chloroplastic, partial [Cucurbita a... [more]
KAG7024230.17.7e-17583.33putative thylakoidal processing peptidase 2, chloroplastic [Cucurbita argyrosper... [more]
XP_022976232.17.7e-17584.41thylakoidal processing peptidase 1, chloroplastic-like [Cucurbita maxima][more]
XP_038900232.17.7e-17585.22thylakoidal processing peptidase 1, chloroplastic-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9M9Z27.5e-8849.07Probable thylakoidal processing peptidase 2, chloroplastic OS=Arabidopsis thalia... [more]
O043483.5e-8551.63Thylakoidal processing peptidase 1, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q8H0W11.4e-5456.25Chloroplast processing peptidase OS=Arabidopsis thaliana OX=3702 GN=PLSP1 PE=2 S... [more]
P726604.6e-2944.38Probable signal peptidase I-1 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX... [more]
P731575.6e-2737.11Probable signal peptidase I-2 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX... [more]
Match NameE-valueIdentityDescription
A0A6J1IF813.7e-17584.41thylakoidal processing peptidase 1, chloroplastic-like OS=Cucurbita maxima OX=36... [more]
A0A6J1FC763.7e-17584.41thylakoidal processing peptidase 1, chloroplastic-like OS=Cucurbita moschata OX=... [more]
A0A6J1CD845.6e-17182.93uncharacterized protein LOC111010591 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1CEX65.6e-17182.93thylakoidal processing peptidase 1, chloroplastic-like isoform X3 OS=Momordica c... [more]
A0A6J1CGI45.6e-17182.93uncharacterized protein LOC111010591 isoform X2 OS=Momordica charantia OX=3673 G... [more]
Match NameE-valueIdentityDescription
AT1G06870.15.3e-8949.07Peptidase S24/S26A/S26B/S26C family protein [more]
AT2G30440.12.5e-8651.63thylakoid processing peptide [more]
AT3G24590.11.0e-5556.25plastidic type i signal peptidase 1 [more]
AT1G23465.14.6e-0836.59Peptidase S24/S26A/S26B/S26C family protein [more]
AT3G08980.16.0e-0842.86Peptidase S24/S26A/S26B/S26C family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000223Peptidase S26A, signal peptidase IPRINTSPR00727LEADERPTASEcoord: 209..225
score: 52.38
coord: 290..309
score: 61.19
IPR000223Peptidase S26A, signal peptidase ITIGRFAMTIGR02227TIGR02227coord: 194..329
e-value: 6.9E-31
score: 105.3
IPR000223Peptidase S26A, signal peptidase IPANTHERPTHR43390SIGNAL PEPTIDASE Icoord: 235..346
IPR000223Peptidase S26A, signal peptidase IPANTHERPTHR43390SIGNAL PEPTIDASE Icoord: 1..235
NoneNo IPR availableGENE3D2.10.109.10Umud Fragment, subunit Acoord: 206..341
e-value: 7.0E-28
score: 99.1
NoneNo IPR availablePANTHERPTHR43390:SF2THYLAKOIDAL PROCESSING PEPTIDASE 2, CHLOROPLASTIC-RELATEDcoord: 1..235
NoneNo IPR availablePANTHERPTHR43390:SF2THYLAKOIDAL PROCESSING PEPTIDASE 2, CHLOROPLASTIC-RELATEDcoord: 235..346
IPR019533Peptidase S26PFAMPF10502Peptidase_S26coord: 193..328
e-value: 3.2E-30
score: 105.3
IPR019533Peptidase S26CDDcd06530S26_SPase_Icoord: 212..322
e-value: 1.01212E-16
score: 72.2331
IPR019758Peptidase S26A, signal peptidase I, conserved sitePROSITEPS00761SPASE_I_3coord: 295..308
IPR019756Peptidase S26A, signal peptidase I, serine active sitePROSITEPS00501SPASE_I_1coord: 218..225
IPR036286LexA/Signal peptidase-like superfamilySUPERFAMILY51306LexA/Signal peptidasecoord: 207..333

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0036913.1Lag0036913.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006465 signal peptide processing
biological_process GO:0010027 thylakoid membrane organization
biological_process GO:0006508 proteolysis
cellular_component GO:0009535 chloroplast thylakoid membrane
cellular_component GO:0005887 integral component of plasma membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity