Lag0036897 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0036897
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptioncytochrome P450 82A3-like
Locationchr2: 1962727 .. 1965639 (-)
RNA-Seq ExpressionLag0036897
SyntenyLag0036897
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTTTCTACTTCCTTCCTTAAACCCATTATCCATTGCAACGCTCATTATCGTGCTCTTCATTTCTTGCTATTGTTTGTCACGAAAGTGGCAGAAGAGAGGAGTCTGTGAGCCAAAGAAGGCCCCTTCGCCGCCGGGAGCTTGGCCTATAATTGGCCATCTCCATCTGCTCAACAATGGGAAGCTCCCTCACCATGTTCTTGGAGCTATGGCGGACAAATATGGGCCGATCTTTCGGTTGCAGCTCGGGTCTCGGTCGGCTCTGGTGGTGAGCAATTGGGAAATGGCCAAAGCAAGCATGTGCGTTAACGATGCGGCTGCTGCGTCTCGCCCGGAACTCTCAGTCTCCAAGCATTTCTCTTATGACTTCGCCATGTTTGGCTTGGCTTCTTACAGCCCTTTCTGGCGCGATATGCGTAAAATAACCCACTTGGAGCTCCTCTCCAACCCTCGTGTCGATCAGGTTTTAAGTTATAAGTCAAAAATTTCAATCCATCTTTAGGCTTTGTTTGATAATTGGATTTTGGTTTTTGAAAGGAATGTACCCAAAATCTCCCTACATACCCCTTTAATATCGTGACTAACTAAGAAAGAGTAAAGGATAAAATGAGATCTTTTTTATCTCTCCTAGTTGTTAACATTCTAATAATGGTATGTAGAAAGATCCTTACACGCTAGTATGCAGTCAAATATATATATATTTTAGATTATGTTTGTTTTCTTCCCCCAATCTCCTTACTATGGTTTTTATCTTTGTTAAGAAGACATTTGAATTTCTAGCCAAATTTTAAAAACTACAATCCCATTTGATAACTATTTGGTTTTTGGTTTTTGAAAATTAAGCTTATTTTCTTTCAATTTCCTTACCATGATATTCATCTTTGTTTATGGCACATTTGAACTCCTAGCTAAATTTCAAAAACTAAGTCAAGTTTATTTTCATAAAAAAAAAAAAAAAAGTCAAGTTTTGAAAACTATTTTCTTTTTAGTTTTCAAAACTTGACTTAGTTTTTTAAAACAAGGGAAAAAAGTATATAACAAAATCAAGAAACTTAATGGTGAAAGTAGTGTTTTTAAATTAATTAATTTTAAAAAACCAAATAGTTGTCAAACATGACCTACTTTTTACTTTTAAAAAACTAACTTGGTTTTTGAAAACATGGAAAAAAAAGTAGATAACATAAAACATAGAAACTTGTGGAAATAGTGTATAAACTAAGCTTCATTTTCAAAAAGTAAAAAGCAAAAATCAAATAGTTATCAAATCGATTTTTAATATGAAACCCAAGATTAATTGTAAAATAAAATGACATTGTAAGAGTTTGAACACACAATAGTTGTTAGCTGAACCTATCACTTTCAAACATTAACAACTATGTGCTGTACATGTCTAAATTGACTATATATATATAAATATATTTATTAATATTCTTAACATCTTTCTACAGTTGGGGATTTGAAAATTAGCACAAAGTTTGAAAATGCGACACGTTGGTTTTGTTTGTTTTTTTTTCTTGCATGAGAAGAAATTGCGTCAAGAGAAACAGATTTTCTTGTAGTGAATACATGAAGGGTGAGAAACCCTTTAATCAGTTGAAGACATGCACAGATTTGTCTGTGTTAAAACATTATAAATTAAATTTTAGCAGTATACTTAATCTTTTTATTTATATTTCTTGAGACAGGTTAAGAGCATCATGTTTGGTGAGCTGGACACATCGTTGACAGAGTTGAACGCAACATGGGCTGCGAAGAAGAACGAGTGGGGCCAGATTTCATTGGAGATGAAGCGTTGGTTTGGGGATGTGATTGTGAATATGCTTCTGAAGGTCATAATAGGAAAACGATGTGTGGGTCCCAACGCCATGGGAGGTGAGGAAGAAGCAAAAGAGTTGCAGATGGCCATTAGAGACTCCTTCCATTTGATGGGTGAAGGTTTACTCAGAGACTACATTCCCATGGTTGCAAAGCTGGGTTTTGACGGACAGGTTAAGGCAATGGAGAAAATAGCAGAACGAATCGATGCGATTATGGGGGTTGGCTCGAAGAACACAATCGTAAGAGAGGTTGTGGAAATTTGGATAAAAAAGATGGAGACTTTAATTTTATGGACTCTTTGCTTTCCTTATCTGATGCCAACGAGTTTCCAAGTTGCTTTGGCCGCGATACCCTTATCAAAGCCACAACCTTGGTACCCCTCTTCTTTGATTATATCATCTTTTAGGTTTCAAATTCTTTTTTTCTTTTATCCAATGATGTTTATACATGTATATGTGCATAGAACATGATTGCAGGAGGAACTGAAAGCAGCACGGTGACTCTAACATGGGCGATGTCGTTGTTACTAAACAACCCACGCGCCCTCCAAATGGCATACCAAGAGCTCGACGAAGTTGTCGGTCGAGAGAGGCGAGTGAAGGAGAATGACATAACCAAGTTAGTTTATTTACAGGCCATAATTAAAGAGACGATGCGAATGTACCCAGCAGGGCCTCTGTTGGGACCTCGTGAGTTCTACAAGGACTGCGTCGTGGCCGGCTATTTTGTCCCCAAAGGCACTCAGCTCATCCCAAACATTTGGAAGATCCAAACCGACCCACGGGTCTGGCCTGACCCGTTTGAGTTCAAGCCGGAGCGGTTTTTGACGACCCATAAGGATGTGGATTTGAAAGGGAATAATTTCGAGTTGATTCCGTTTGGGAGTGGGCGTAGAGGGTGTCCTGGAGTTGCGTTTGGGCTTCAAATGGTGCATTTCACTTTGGCTGGGTTTTTACAGTCTTTTGATGTTAAAAGCCGGACCGGTGAACCGGTTGATATGGCGGAGCATTTCGGAATGGCCAATGAGAAAGTAGTGCCTCTCAATGTTTTGGTCACCCCGCGGTTACCTTCGCATCTTCACGGCCCGATGTAG

mRNA sequence

ATGGAGTTTCTACTTCCTTCCTTAAACCCATTATCCATTGCAACGCTCATTATCGTGCTCTTCATTTCTTGCTATTGTTTGTCACGAAAGTGGCAGAAGAGAGGAGTCTGTGAGCCAAAGAAGGCCCCTTCGCCGCCGGGAGCTTGGCCTATAATTGGCCATCTCCATCTGCTCAACAATGGGAAGCTCCCTCACCATGTTCTTGGAGCTATGGCGGACAAATATGGGCCGATCTTTCGGTTGCAGCTCGGGTCTCGGTCGGCTCTGGTGGTGAGCAATTGGGAAATGGCCAAAGCAAGCATGTGCGTTAACGATGCGGCTGCTGCGTCTCGCCCGGAACTCTCAGTCTCCAAGCATTTCTCTTATGACTTCGCCATGTTTGGCTTGGCTTCTTACAGCCCTTTCTGGCGCGATATGCGTAAAATAACCCACTTGGAGCTCCTCTCCAACCCTCGTGTCGATCAGGTTAAGAGCATCATGTTTGGTGAGCTGGACACATCGTTGACAGAGTTGAACGCAACATGGGCTGCGAAGAAGAACGAGTGGGGCCAGATTTCATTGGAGATGAAGCGTTGGTTTGGGGATGTGATTGTGAATATGCTTCTGAAGGTCATAATAGGAAAACGATGTGTGGGTCCCAACGCCATGGGAGGTGAGGAAGAAGCAAAAGAGTTGCAGATGGCCATTAGAGACTCCTTCCATTTGATGGGTGAAGGTTTACTCAGAGACTACATTCCCATGGTTGCAAAGCTGGGTTTTGACGGACAGGTTAAGGCAATGGAGAAAATAGCAGAACGAATCGATGCGATTATGGGGGTTGGCTCGAAGAACACAATCAACATGATTGCAGGAGGAACTGAAAGCAGCACGGTGACTCTAACATGGGCGATGTCGTTGTTACTAAACAACCCACGCGCCCTCCAAATGGCATACCAAGAGCTCGACGAAGTTGTCGGTCGAGAGAGGCGAGTGAAGGAGAATGACATAACCAAGTTAGTTTATTTACAGGCCATAATTAAAGAGACGATGCGAATGTACCCAGCAGGGCCTCTGTTGGGACCTCGTGAGTTCTACAAGGACTGCGTCGTGGCCGGCTATTTTGTCCCCAAAGGCACTCAGCTCATCCCAAACATTTGGAAGATCCAAACCGACCCACGGGTCTGGCCTGACCCGTTTGAGTTCAAGCCGGAGCGGTTTTTGACGACCCATAAGGATGTGGATTTGAAAGGGAATAATTTCGAGTTGATTCCGTTTGGGAGTGGGCGTAGAGGGTGTCCTGGAGTTGCGTTTGGGCTTCAAATGGTGCATTTCACTTTGGCTGGGTTTTTACAGTCTTTTGATGTTAAAAGCCGGACCGGTGAACCGGTTGATATGGCGGAGCATTTCGGAATGGCCAATGAGAAAGTAGTGCCTCTCAATGTTTTGGTCACCCCGCGGTTACCTTCGCATCTTCACGGCCCGATGTAG

Coding sequence (CDS)

ATGGAGTTTCTACTTCCTTCCTTAAACCCATTATCCATTGCAACGCTCATTATCGTGCTCTTCATTTCTTGCTATTGTTTGTCACGAAAGTGGCAGAAGAGAGGAGTCTGTGAGCCAAAGAAGGCCCCTTCGCCGCCGGGAGCTTGGCCTATAATTGGCCATCTCCATCTGCTCAACAATGGGAAGCTCCCTCACCATGTTCTTGGAGCTATGGCGGACAAATATGGGCCGATCTTTCGGTTGCAGCTCGGGTCTCGGTCGGCTCTGGTGGTGAGCAATTGGGAAATGGCCAAAGCAAGCATGTGCGTTAACGATGCGGCTGCTGCGTCTCGCCCGGAACTCTCAGTCTCCAAGCATTTCTCTTATGACTTCGCCATGTTTGGCTTGGCTTCTTACAGCCCTTTCTGGCGCGATATGCGTAAAATAACCCACTTGGAGCTCCTCTCCAACCCTCGTGTCGATCAGGTTAAGAGCATCATGTTTGGTGAGCTGGACACATCGTTGACAGAGTTGAACGCAACATGGGCTGCGAAGAAGAACGAGTGGGGCCAGATTTCATTGGAGATGAAGCGTTGGTTTGGGGATGTGATTGTGAATATGCTTCTGAAGGTCATAATAGGAAAACGATGTGTGGGTCCCAACGCCATGGGAGGTGAGGAAGAAGCAAAAGAGTTGCAGATGGCCATTAGAGACTCCTTCCATTTGATGGGTGAAGGTTTACTCAGAGACTACATTCCCATGGTTGCAAAGCTGGGTTTTGACGGACAGGTTAAGGCAATGGAGAAAATAGCAGAACGAATCGATGCGATTATGGGGGTTGGCTCGAAGAACACAATCAACATGATTGCAGGAGGAACTGAAAGCAGCACGGTGACTCTAACATGGGCGATGTCGTTGTTACTAAACAACCCACGCGCCCTCCAAATGGCATACCAAGAGCTCGACGAAGTTGTCGGTCGAGAGAGGCGAGTGAAGGAGAATGACATAACCAAGTTAGTTTATTTACAGGCCATAATTAAAGAGACGATGCGAATGTACCCAGCAGGGCCTCTGTTGGGACCTCGTGAGTTCTACAAGGACTGCGTCGTGGCCGGCTATTTTGTCCCCAAAGGCACTCAGCTCATCCCAAACATTTGGAAGATCCAAACCGACCCACGGGTCTGGCCTGACCCGTTTGAGTTCAAGCCGGAGCGGTTTTTGACGACCCATAAGGATGTGGATTTGAAAGGGAATAATTTCGAGTTGATTCCGTTTGGGAGTGGGCGTAGAGGGTGTCCTGGAGTTGCGTTTGGGCTTCAAATGGTGCATTTCACTTTGGCTGGGTTTTTACAGTCTTTTGATGTTAAAAGCCGGACCGGTGAACCGGTTGATATGGCGGAGCATTTCGGAATGGCCAATGAGAAAGTAGTGCCTCTCAATGTTTTGGTCACCCCGCGGTTACCTTCGCATCTTCACGGCCCGATGTAG

Protein sequence

MEFLLPSLNPLSIATLIIVLFISCYCLSRKWQKRGVCEPKKAPSPPGAWPIIGHLHLLNNGKLPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKASMCVNDAAAASRPELSVSKHFSYDFAMFGLASYSPFWRDMRKITHLELLSNPRVDQVKSIMFGELDTSLTELNATWAAKKNEWGQISLEMKRWFGDVIVNMLLKVIIGKRCVGPNAMGGEEEAKELQMAIRDSFHLMGEGLLRDYIPMVAKLGFDGQVKAMEKIAERIDAIMGVGSKNTINMIAGGTESSTVTLTWAMSLLLNNPRALQMAYQELDEVVGRERRVKENDITKLVYLQAIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPNIWKIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFDVKSRTGEPVDMAEHFGMANEKVVPLNVLVTPRLPSHLHGPM
Homology
BLAST of Lag0036897 vs. NCBI nr
Match: XP_022976389.1 (cytochrome P450 82A3-like [Cucurbita maxima])

HSP 1 Score: 817.4 bits (2110), Expect = 6.7e-233
Identity = 408/532 (76.69%), Postives = 445/532 (83.65%), Query Frame = 0

Query: 1   MEFLLPSLNPLSIATLIIVLFISCYCLSRKWQKRGVCEPKKAPSPPGAWPIIGHLHLLNN 60
           MEF LP LNPLSI  L I+   SCY LSRKW KRGVCEPKKAPSPPGAWPIIGHLHLLN+
Sbjct: 1   MEFPLPYLNPLSIILLFII--SSCY-LSRKWVKRGVCEPKKAPSPPGAWPIIGHLHLLNS 60

Query: 61  GKLPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKASMCVNDAAAASRPELSVSKHF 120
           GKLPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAK SMC+ND AAASRPELSVSK+F
Sbjct: 61  GKLPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKESMCINDGAAASRPELSVSKNF 120

Query: 121 SYDFAMFGLASYSPFWRDMRKITHLELLSNPRVDQVKSIMFGELDTSLTELNATWAAKKN 180
           SY FAMFGLASY  +WRDMRKITHL+LLSNPRVDQVKS+M  E+ TS+TEL+A WA++KN
Sbjct: 121 SYHFAMFGLASYGTYWRDMRKITHLQLLSNPRVDQVKSMMLSEMATSVTELHARWASEKN 180

Query: 181 EWGQISLEMKRWFGDVIVNMLLKVIIGKRCVGPNAMGGEEEAKELQMAIRDSFHLMGEGL 240
           + G I +EMKRWFGDV VNMLLKVIIGKRCVGPNA G E+EA +LQMAIR SFHLMGEGL
Sbjct: 181 QLGHIKVEMKRWFGDVTVNMLLKVIIGKRCVGPNAKGDEKEAIDLQMAIRKSFHLMGEGL 240

Query: 241 LRDYIPMVAKLGFDGQVKAMEKIAERIDAIMGV--------GSKN--------------- 300
           LRDYIP++A +GFD  VKAMEKIA+RID+I+          G KN               
Sbjct: 241 LRDYIPILANMGFDRHVKAMEKIAKRIDSILQCWLEEHRRNGDKNQSLDTKDGDFMDSLL 300

Query: 301 ---------------------TINMIAGGTESSTVTLTWAMSLLLNNPRALQMAYQELDE 360
                                T+NMIAGGTESSTVTLTWA+SLL+NNPRAL+ AY+ELD+
Sbjct: 301 SLCRANELPSHFDHDTIVKATTLNMIAGGTESSTVTLTWALSLLVNNPRALEKAYEELDQ 360

Query: 361 VVGRERRVKENDITKLVYLQAIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIP 420
           VVGR+RRVKE+DITKLVYLQA++KETMR+YPAGPLLGPREFYKDC VAGYFVPKGTQLIP
Sbjct: 361 VVGRDRRVKESDITKLVYLQAVVKETMRLYPAGPLLGPREFYKDCFVAGYFVPKGTQLIP 420

Query: 421 NIWKIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPGVAFGLQMVH 480
           NIWKIQTDPRVW DPFEFKPERFLTTH DVDLKGNNFELIPFGSGRRGCP VAFGLQMVH
Sbjct: 421 NIWKIQTDPRVWADPFEFKPERFLTTHCDVDLKGNNFELIPFGSGRRGCPAVAFGLQMVH 480

Query: 481 FTLAGFLQSFDVKSRTGEPVDMAEHFGMANEKVVPLNVLVTPRLPSHLHGPM 489
           F LAGFL SF VK+ T +PVDMAE+FGMANEKVVPLNVLVTPRLPSHLHGP+
Sbjct: 481 FALAGFLHSFTVKTPTPQPVDMAENFGMANEKVVPLNVLVTPRLPSHLHGPI 529

BLAST of Lag0036897 vs. NCBI nr
Match: XP_023535454.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 803.9 bits (2075), Expect = 7.6e-229
Identity = 400/532 (75.19%), Postives = 439/532 (82.52%), Query Frame = 0

Query: 1   MEFLLPSLNPLSIATLIIVLFISCYCLSRKWQKRGVCEPKKAPSPPGAWPIIGHLHLLNN 60
           MEF LP LNPLSI  L I+   SCY LSRKW KRGVCEPKKAPSPPGAWPIIGHLHLLNN
Sbjct: 1   MEFPLPYLNPLSIILLFII--SSCY-LSRKWVKRGVCEPKKAPSPPGAWPIIGHLHLLNN 60

Query: 61  GKLPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKASMCVNDAAAASRPELSVSKHF 120
           GKLPHHVLGAMADKYGPIF LQLGSR ALVVSNWEMAK SMC+ND AAASRPELSVS++F
Sbjct: 61  GKLPHHVLGAMADKYGPIFHLQLGSRPALVVSNWEMAKESMCINDGAAASRPELSVSRNF 120

Query: 121 SYDFAMFGLASYSPFWRDMRKITHLELLSNPRVDQVKSIMFGELDTSLTELNATWAAKKN 180
           SY FAMFGLASYS +WRDMRKITHL LLSNP VDQVKS+M  E+ TS+TEL+A WA++KN
Sbjct: 121 SYHFAMFGLASYSSYWRDMRKITHLHLLSNPCVDQVKSMMLSEMATSVTELHARWASEKN 180

Query: 181 EWGQISLEMKRWFGDVIVNMLLKVIIGKRCVGPNAMGGEEEAKELQMAIRDSFHLMGEGL 240
           + G I +EMKRWFGDV +NMLLKVIIGKRCVGPNA G E+EA +LQMAIR SFHLMGEG+
Sbjct: 181 QLGHIKVEMKRWFGDVTLNMLLKVIIGKRCVGPNAKGDEKEAIDLQMAIRKSFHLMGEGM 240

Query: 241 LRDYIPMVAKLGFDGQVKAMEKIAERIDAIM----------------------------- 300
           LRDYIP++A LGFD   KAMEKIA RID+I+                             
Sbjct: 241 LRDYIPILANLGFDRHGKAMEKIAIRIDSILQSWLQEHRRNTDKNQSLDTKDGDFMDSLL 300

Query: 301 ---------------GVGSKNTINMIAGGTESSTVTLTWAMSLLLNNPRALQMAYQELDE 360
                           +    T+NMIAGGTESS+VTLTWA+SLL+NNPRAL+ AY+ELD+
Sbjct: 301 SLCRSNELPSHFDHDTIVKATTLNMIAGGTESSSVTLTWALSLLVNNPRALEKAYEELDQ 360

Query: 361 VVGRERRVKENDITKLVYLQAIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIP 420
           VVGR+RRVKE+DITKLVYLQA++KETMR+YPAGPLLGPREFYKDC VAGYFVPKGTQLIP
Sbjct: 361 VVGRDRRVKESDITKLVYLQAVVKETMRLYPAGPLLGPREFYKDCFVAGYFVPKGTQLIP 420

Query: 421 NIWKIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPGVAFGLQMVH 480
           NIWKIQTDPRVWPDPFEFKPERFLTTH DVDLKGNNFELIPFGSGRRGCP VAFGLQMVH
Sbjct: 421 NIWKIQTDPRVWPDPFEFKPERFLTTHCDVDLKGNNFELIPFGSGRRGCPAVAFGLQMVH 480

Query: 481 FTLAGFLQSFDVKSRTGEPVDMAEHFGMANEKVVPLNVLVTPRLPSHLHGPM 489
           F LAGFL SF VK+ T +PVDMAE+FGMANEKVVPL+VLVTPRLPSHLHGP+
Sbjct: 481 FALAGFLHSFTVKTPTPQPVDMAENFGMANEKVVPLDVLVTPRLPSHLHGPI 529

BLAST of Lag0036897 vs. NCBI nr
Match: XP_022935738.1 (cytochrome P450 82A3-like [Cucurbita moschata])

HSP 1 Score: 795.0 bits (2052), Expect = 3.5e-226
Identity = 396/532 (74.44%), Postives = 433/532 (81.39%), Query Frame = 0

Query: 1   MEFLLPSLNPLSIATLIIVLFISCYCLSRKWQKRGVCEPKKAPSPPGAWPIIGHLHLLNN 60
           MEF LP LNPLSI  L I+   SCY LSRKW KRGVCE KKAPSPPGAWPIIGHLHLLNN
Sbjct: 1   MEFPLPYLNPLSIILLFII--SSCY-LSRKWVKRGVCEAKKAPSPPGAWPIIGHLHLLNN 60

Query: 61  GKLPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKASMCVNDAAAASRPELSVSKHF 120
           GKLPH VLGAMADKYGPIF LQLGSR ALVVSNWEMAK SMC+ND AAASRPELSVSK+F
Sbjct: 61  GKLPHRVLGAMADKYGPIFHLQLGSRPALVVSNWEMAKESMCINDGAAASRPELSVSKNF 120

Query: 121 SYDFAMFGLASYSPFWRDMRKITHLELLSNPRVDQVKSIMFGELDTSLTELNATWAAKKN 180
           SY FAMFGLASYSP+WRDMRKI+HL LLSN RVDQ KS+M  E+ TS+TEL+  WA++KN
Sbjct: 121 SYHFAMFGLASYSPYWRDMRKISHLHLLSNGRVDQAKSMMLSEMATSVTELHTRWASEKN 180

Query: 181 EWGQISLEMKRWFGDVIVNMLLKVIIGKRCVGPNAMGGEEEAKELQMAIRDSFHLMGEGL 240
           + G I +EMKRWFGDV +NMLLKVIIGKRCVGPNA G E+EA +LQMAIR SFHLMGEG+
Sbjct: 181 QLGHIKVEMKRWFGDVTLNMLLKVIIGKRCVGPNAKGDEKEAIDLQMAIRKSFHLMGEGM 240

Query: 241 LRDYIPMVAKLGFDGQVKAMEKIAERIDAIM----------------------------- 300
           LRDYIP++A LGFD  VKAMEKIA RID+I+                             
Sbjct: 241 LRDYIPILANLGFDRHVKAMEKIAIRIDSILQGWLQEHRRNKAKNQSLDTKDGDFMDSLL 300

Query: 301 ---------------GVGSKNTINMIAGGTESSTVTLTWAMSLLLNNPRALQMAYQELDE 360
                           +     +NMIAGGTESS+VTLTWA+SLL+NNP AL+ AY+ELD+
Sbjct: 301 SLCRSNELPSQFDHDTIVKATILNMIAGGTESSSVTLTWALSLLVNNPGALEKAYEELDQ 360

Query: 361 VVGRERRVKENDITKLVYLQAIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIP 420
           VVGR+RRV E+DITKLVYLQA++KETMR+YPAGPLLGPREFYKDC VAGYFVPKGTQLIP
Sbjct: 361 VVGRDRRVNESDITKLVYLQAVVKETMRLYPAGPLLGPREFYKDCFVAGYFVPKGTQLIP 420

Query: 421 NIWKIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPGVAFGLQMVH 480
           NIWKIQTDPRVWPDPFEFKPERFLTTH DVDLKGNNFELIPFGSGRRGCP VAFGLQMVH
Sbjct: 421 NIWKIQTDPRVWPDPFEFKPERFLTTHCDVDLKGNNFELIPFGSGRRGCPAVAFGLQMVH 480

Query: 481 FTLAGFLQSFDVKSRTGEPVDMAEHFGMANEKVVPLNVLVTPRLPSHLHGPM 489
           F LAG L SF VK+ T +PVDMAEHFGMANEKVVPLNVLVTPRLPSHLHGP+
Sbjct: 481 FALAGLLHSFTVKTPTPQPVDMAEHFGMANEKVVPLNVLVTPRLPSHLHGPI 529

BLAST of Lag0036897 vs. NCBI nr
Match: XP_022936278.1 (cytochrome P450 CYP82D47-like [Cucurbita moschata])

HSP 1 Score: 793.1 bits (2047), Expect = 1.3e-225
Identity = 393/532 (73.87%), Postives = 435/532 (81.77%), Query Frame = 0

Query: 1   MEFLLPSLNPLSIATLIIVLFISCYCLSRKWQKRGVCEPKKAPSPPGAWPIIGHLHLLNN 60
           MEF LP LNPLSI  L I+   SCY LSRKW KRGVCE KK PSPPGAWPIIGHLHLLNN
Sbjct: 1   MEFPLPYLNPLSIILLFII--SSCY-LSRKWVKRGVCEAKKVPSPPGAWPIIGHLHLLNN 60

Query: 61  GKLPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKASMCVNDAAAASRPELSVSKHF 120
           GKLPH VLGAMADKYGPIF LQ+GSR ALVVSNWEMAK SMC+ND AAASRPELSVSK+F
Sbjct: 61  GKLPHRVLGAMADKYGPIFHLQIGSRPALVVSNWEMAKESMCINDGAAASRPELSVSKNF 120

Query: 121 SYDFAMFGLASYSPFWRDMRKITHLELLSNPRVDQVKSIMFGELDTSLTELNATWAAKKN 180
           SY FAMFGLASYSP+WRDMRKI+HL LLSN RVDQVKS+M  E+ TS+TEL+A WA++KN
Sbjct: 121 SYHFAMFGLASYSPYWRDMRKISHLHLLSNRRVDQVKSMMLSEMATSVTELHARWASEKN 180

Query: 181 EWGQISLEMKRWFGDVIVNMLLKVIIGKRCVGPNAMGGEEEAKELQMAIRDSFHLMGEGL 240
           + G I++EMKRWFGDVI NMLLKVIIGKRCVGPNA G E+EA +LQMAI  SFHLMGEG+
Sbjct: 181 QMGHINVEMKRWFGDVIANMLLKVIIGKRCVGPNAKGDEKEAIDLQMAIGKSFHLMGEGM 240

Query: 241 LRDYIPMVAKLGFDGQVKAMEKIAERIDAIM----------------------------- 300
           LRDYIP++A LGFD   KAMEKIA RID+I+                             
Sbjct: 241 LRDYIPILANLGFDRHSKAMEKIATRIDSILQGWLQEHRRNRDKNQSLDTKDGDFMDSLL 300

Query: 301 ---------------GVGSKNTINMIAGGTESSTVTLTWAMSLLLNNPRALQMAYQELDE 360
                           +    T+NMIAGGTE+STVTLTWA+SLL+NNP AL+ AY+ELD+
Sbjct: 301 SLCRTNELPRHFDHDTIVKATTLNMIAGGTETSTVTLTWALSLLVNNPGALEKAYEELDQ 360

Query: 361 VVGRERRVKENDITKLVYLQAIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIP 420
           VVGR+RRV E+DITKLVYLQA++KETMR+YPAGPLLGPREFYKDC VAGYFVPKGTQLIP
Sbjct: 361 VVGRDRRVNESDITKLVYLQAVVKETMRLYPAGPLLGPREFYKDCFVAGYFVPKGTQLIP 420

Query: 421 NIWKIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPGVAFGLQMVH 480
           NIWKIQTDPRVWPDPFEFKPERFLTTH DVDLKGNNFELIPFGSGRRGCPG+AFGLQMVH
Sbjct: 421 NIWKIQTDPRVWPDPFEFKPERFLTTHCDVDLKGNNFELIPFGSGRRGCPGIAFGLQMVH 480

Query: 481 FTLAGFLQSFDVKSRTGEPVDMAEHFGMANEKVVPLNVLVTPRLPSHLHGPM 489
           F LAGFL SF+VK+ T + +DMAE FGMANEK+VPLNVLVTPRLPSHLHGP+
Sbjct: 481 FALAGFLHSFNVKTPTPQSIDMAEGFGMANEKLVPLNVLVTPRLPSHLHGPI 529

BLAST of Lag0036897 vs. NCBI nr
Match: XP_022149464.1 (cytochrome P450 82A3-like [Momordica charantia])

HSP 1 Score: 775.0 bits (2000), Expect = 3.8e-220
Identity = 380/518 (73.36%), Postives = 428/518 (82.63%), Query Frame = 0

Query: 10  PLSIATLIIVLFISCYCLSRKWQKRGVCEPKKAPSPPGAWPIIGHLHLLNNGKLPHHVLG 69
           P+ IA + ++  +  Y L RK Q     + K APSPPGAWP+IGHLHLL+N KLPHH LG
Sbjct: 16  PICIAAIFLIFIVCYYYLCRKRQD----QRKLAPSPPGAWPVIGHLHLLHNAKLPHHTLG 75

Query: 70  AMADKYGPIFRLQLGSRSALVVSNWEMAKASMCVNDAAAASRPELSVSKHFSYDFAMFGL 129
           AMADKYGPIFRLQLGSRSALVVSNWEMAK SMC+NDAAAASRPELSVSKHFSYDFAMFGL
Sbjct: 76  AMADKYGPIFRLQLGSRSALVVSNWEMAKESMCINDAAAASRPELSVSKHFSYDFAMFGL 135

Query: 130 ASYSPFWRDMRKITHLELLSNPRVDQVKSIMFGELDTSLTELNATWAAKKNEWGQISLEM 189
           ASY P+WR+MRKITHLELLSNPRV +VK+IMFGE+D SLTEL+A W  ++NE GQI +E+
Sbjct: 136 ASYGPYWREMRKITHLELLSNPRVQEVKNIMFGEVDMSLTELHAKWVVQRNESGQIPVEL 195

Query: 190 KRWFGDVIVNMLLKVIIGKRCVGPNAMGGEEEAKELQMAIRDSFHLMGEGLLRDYIPMVA 249
           K WFGDV++NMLLK+IIGKRCVGPNA+GGE EAKELQMAIR+SF LMG+G+LRD+IP+VA
Sbjct: 196 KGWFGDVVMNMLLKIIIGKRCVGPNALGGEAEAKELQMAIRESFRLMGQGILRDFIPVVA 255

Query: 250 KLGFDGQVKAMEKIAERIDAIM-------------GVGSKN------------------- 309
           +LGFDGQVKAMEKIA+RIDAI+             G+  KN                   
Sbjct: 256 QLGFDGQVKAMEKIAKRIDAILEGWLQDHKKNRAYGLAKKNGDFMVSLLSLYDANQLPSQ 315

Query: 310 ----------TINMIAGGTESSTVTLTWAMSLLLNNPRALQMAYQELDEVVGRERRVKEN 369
                     T+NMIAGGTESSTVTLTWAMSLLLNNP AL+ AYQELD VVGR+RRV E+
Sbjct: 316 YNGDIITKATTLNMIAGGTESSTVTLTWAMSLLLNNPHALEKAYQELDAVVGRDRRVNES 375

Query: 370 DITKLVYLQAIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPNIWKIQTDPRV 429
           DI+KL YLQAI+KETMR+YPAGPLLGPREFYKDCVV GYFVPKGTQLIPNIWKIQ DPRV
Sbjct: 376 DISKLSYLQAIVKETMRLYPAGPLLGPREFYKDCVVGGYFVPKGTQLIPNIWKIQVDPRV 435

Query: 430 WPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFD 486
           WPDP +FKPERFLTTHK+VD+KGN+FELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFD
Sbjct: 436 WPDPLKFKPERFLTTHKEVDVKGNDFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFD 495

BLAST of Lag0036897 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 3.0e-127
Identity = 231/516 (44.77%), Postives = 322/516 (62.40%), Query Frame = 0

Query: 7   SLNPLSIATLIIVLFISCYCLSRKWQKRGVCEPKKAPSPPGAWPIIGHLHLLNNGKLPHH 66
           +LN ++IA+++ ++F+ C  L RK   RG    K AP   GAWPI+GHL LLN  + PH 
Sbjct: 9   NLNTIAIASILSLIFL-CLFLYRK-NSRG----KDAPVVSGAWPILGHLSLLNGSQTPHK 68

Query: 67  VLGAMADKYGPIFRLQLGSRSALVVSNWEMAKASMCVNDAAAASRPELSVSKHFSYDFAM 126
           VLGA+ADKYGP+F ++LG + ALV+SNWEM+K     ND A +SRP+L   +  SY+ A 
Sbjct: 69  VLGALADKYGPLFTIKLGMKPALVLSNWEMSKELFTTNDLAVSSRPKLVAVEVMSYNQAF 128

Query: 127 FGLASYSPFWRDMRKITHLELLSNPRVDQVKSIMFGELDTSLTELNATWA-AKKNEWGQI 186
            GLA Y P+WR++RKI   E LSN R++Q   I   E+ TS+ EL   W+   KNE    
Sbjct: 129 VGLAPYGPYWRELRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNESRYT 188

Query: 187 SLEMKRWFGDVIVNMLLKVIIGKRCVGPNAMGGEEEAKELQMAIRDSFHLMGEGLLRDYI 246
            +++K+W   +  NM++++++GKR  G   + G+++A+     IR+  +LMG   + D +
Sbjct: 189 LVDIKQWLAYLTFNMVVRMVVGKRYFGVMHVEGKDKAQRFMKNIREFMNLMGTFTVADGV 248

Query: 247 PMVAKLGFDGQVKAMEKIAERIDAIMG--------------------------------- 306
           P +  L   G  KAM+  A+ +D ++                                  
Sbjct: 249 PCLRWLDLGGHEKAMKANAKEVDKLLSEWLEEHRQKKLLGENVESDRDFMDVMISALNGA 308

Query: 307 ---------VGSKNTINMIAGGTESSTVTLTWAMSLLLNNPRALQMAYQELDEVVGRERR 366
                    +    ++ +I GGT+S+ VTLTWA+SLLL NP AL  A +E+D  +G++  
Sbjct: 309 QIGAFDADTICKATSLELILGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDMQIGKDEY 368

Query: 367 VKENDITKLVYLQAIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPNIWKIQT 426
           ++E+DI+KLVYLQAI+KET+R+YP  P   PREF ++C++ GY + KGT+LI N+WKI  
Sbjct: 369 IRESDISKLVYLQAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHNLWKIHR 428

Query: 427 DPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPGVAFGLQMVHFTLAGFL 480
           DP VW DP EFKPERFLTTHKDVDL+G+NFEL+PFGSGRR C G++ GL MVHFTLA  L
Sbjct: 429 DPSVWSDPLEFKPERFLTTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVHFTLANLL 488

BLAST of Lag0036897 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 7.2e-121
Identity = 224/522 (42.91%), Postives = 321/522 (61.49%), Query Frame = 0

Query: 1   MEFLLPSLNPLSIATLIIVLFISCYCLSRKWQKRGVCEPKKAPSPPGAWPIIGHLHLLNN 60
           ME +L  LN  +I  + ++  +  +      +  G    K+AP+  GAWPI GHL LL  
Sbjct: 1   MELVLHFLNTTTIGVVSLIFLLCLFLYGPLKKVHG--SSKEAPTVGGAWPIFGHLPLLIG 60

Query: 61  GKLPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKASMCVNDAAAASRPELSVSKHF 120
            K PH  LGA+A+K+GP+F ++LG++ ALVVS+WEMA+     ND A ++RP+L V++  
Sbjct: 61  SKSPHKALGALAEKHGPLFTIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLLVAELM 120

Query: 121 SYDFAMFGLASYSPFWRDMRKITHLELLSNPRVDQVKSIMFGELDTSLTELNATWAAKKN 180
            Y+ AM  +A Y P+WR++RKI   E+LS+ RV+Q++ +   E+  S+ EL   W ++KN
Sbjct: 121 CYNNAMLLVAPYGPYWRELRKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDVWRSQKN 180

Query: 181 EWGQISLEMKRWFGDVIVNMLLKVIIGKRCVGPNAMGGEEEAKELQMAIRDSFHLMGEGL 240
           E    S+E+K+WF   I NM+L++++GKR +  +A   +E+A++   A+ +   L G   
Sbjct: 181 ESDYASVELKQWFAQPIFNMVLRMVVGKRFL--SATATDEKAEKCVKAVDEFMRLAGVFT 240

Query: 241 LRDYIPMVAKLGFDGQVKAMEKIAERIDAI-------------MGVG------------- 300
           + D IP +  L F G  KAM++ A+ +D +             +G G             
Sbjct: 241 VGDAIPYLRWLDFGGYEKAMKETAKELDVMISEWLEEHRQKRALGEGVDGAQDFMNVMLS 300

Query: 301 ----------------SKNTINMIAGGTESSTVTLTWAMSLLLNNPRALQMAYQELDEVV 360
                               + +I  GTE+S  T+ WAM L+L NP  L+    ELD  V
Sbjct: 301 SLDGKTIDGIDADTLIKSTVLTIIQAGTEASISTIIWAMCLILKNPLILENK-AELDIQV 360

Query: 361 GRERRVKENDITKLVYLQAIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPNI 420
           G++R + E+DI+ LVYLQA++KET+R+Y  GPL  PREF +DC + GY V KGT+LI NI
Sbjct: 361 GKDRCICESDISNLVYLQAVVKETLRLYAPGPLSSPREFAEDCTLGGYHVKKGTRLITNI 420

Query: 421 WKIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPGVAFGLQMVHFT 480
           WKI TDP VW DPFEFKP+RFLTTHKD+D+KG++F+L+PFGSGRR CPG++FGLQ VH  
Sbjct: 421 WKIHTDPNVWSDPFEFKPDRFLTTHKDIDVKGHHFQLLPFGSGRRVCPGISFGLQTVHLA 480

BLAST of Lag0036897 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 422.5 bits (1085), Expect = 6.3e-117
Identity = 218/536 (40.67%), Postives = 312/536 (58.21%), Query Frame = 0

Query: 8   LNPLSIATLIIVLFISCYCLSRKWQKRGVCEPKKAPSPPGAWPIIGHLHLLNNGKLPHHV 67
           LN L+  T+  +  IS      ++ K  V   K+ P   G+WP++GHL L+ N + PH  
Sbjct: 3   LNYLNTTTIAFISLISLLFFLFRFSK--VSHTKEPPIISGSWPLLGHLPLMRNTQTPHKT 62

Query: 68  LGAMADKYGPIFRLQLGSRSALVVSNWEMAKASMCVNDAAAASRPELSVSKHFSYDFAMF 127
           LGA+ DKYGPIF ++LG+ +ALV+SNWE+AK     ND   +SRP+    +  SY+ A  
Sbjct: 63  LGALVDKYGPIFTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYNQAFI 122

Query: 128 GLASYSPFWRDMRKITHLELLSNPRVDQVKSIMFGELDTSLTELNATWAAK-KNEWG--- 187
           G A Y  +WR +RKI  LE+LSN R++ +  I   E+ TS+ EL   W+ +  +++G   
Sbjct: 123 GWAPYGAYWRQLRKIVTLEILSNRRIELLSHIRVSEVQTSIKELVNVWSNQISSQYGLLD 182

Query: 188 ---------------QISLEMKRWFGDVIVNMLLKVIIGKRCVGPNAMGGEEEAKELQMA 247
                           +S+E+K+WF  + +NM+L++++GKRC G   +  +EEAK     
Sbjct: 183 DTKSSSTNDEPSTTDYVSVELKKWFAQLTLNMVLRMVVGKRCFGDVDVENKEEAKRFLEN 242

Query: 248 IRDSFHLMGEGLLRDYIPMVAKLGFDGQVKAMEKIAERIDAIM-----------GVGSKN 307
           IRD   L+G   + D +P +  L   G  K M+K A++ D ++           G+GS++
Sbjct: 243 IRDFMRLIGTFTVGDGVPFLKWLDLGGHEKEMKKCAKKFDVMLNEWLEEHREKKGLGSED 302

Query: 308 ---------------------------------TINMIAGGTESSTVTLTWAMSLLLNNP 367
                                            T+ +I GG++++  TLTWAM LLL +P
Sbjct: 303 KVVGERDFMDAMLLVLKDKPIEGFDVDTIIKATTLELILGGSDTTAGTLTWAMCLLLKHP 362

Query: 368 RALQMAYQELDEVVGRERRVKENDITKLVYLQAIIKETMRMYPAGPLLGPREFYKDCVVA 427
             L+   +EL+  +G+ER V E+DI KLVYL AIIKET+R+YP  P   PREF +DC + 
Sbjct: 363 HVLEKLKEELNTYIGKERCVNESDINKLVYLHAIIKETLRLYPPAPFSSPREFTEDCTIG 422

Query: 428 GYFVPKGTQLIPNIWKIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRG 481
           GY + KGT+L+PN+WKI  DP VWPDP EFKPERFL+THKDVD++G NFEL+PFGSGRR 
Sbjct: 423 GYHIKKGTRLMPNLWKIHRDPSVWPDPLEFKPERFLSTHKDVDVRGQNFELLPFGSGRRM 482

BLAST of Lag0036897 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 1.7e-114
Identity = 225/520 (43.27%), Postives = 312/520 (60.00%), Query Frame = 0

Query: 16  LIIVLFISCYCLSRKWQKRGVCEPK--KAPSPPGAWPIIGHLHLL-NNGKLPHHVLGAMA 75
           +++ +FI+ +  S+K        PK  KAP+P GAWPIIGHLHLL    +L +  LG MA
Sbjct: 12  ILVFVFIALFKKSKK--------PKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMA 71

Query: 76  DKYGPIFRLQLGSRSALVVSNWEMAKASMCVNDAAAASRPELSVSKHFSYDFAMFGLASY 135
           D YGP   LQLGS  A VVS++E+AK    VND A ASRP  + +KH  Y+FA+FG A Y
Sbjct: 72  DHYGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAPY 131

Query: 136 SPFWRDMRKITHLELLSNPRVDQVKSIMFGELDTSLTELNATWAAKKNEWGQISLEMKRW 195
           S FWR+MRKI  +ELLSN R+  +K +   E+   + +L + W  K      + +++K W
Sbjct: 132 SAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLW-FKNGGTKPVMVDLKSW 191

Query: 196 FGDVIVNMLLKVIIGKRCVGPN---AMGGEEEAKELQMAIRDSFHLMGEGLLRDYIPMVA 255
             D+ +NM+++++ GKR  G     +    EEA + + AI   FHL+G   + D  P ++
Sbjct: 192 LEDMTLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLS 251

Query: 256 KLGFDGQVKAMEKIAERIDAIM-------------------------------------- 315
                G  K M++    +D I+                                      
Sbjct: 252 FFDLQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAEQGKLSH 311

Query: 316 -----GVGSKNT-INMIAGGTESSTVTLTWAMSLLLNNPRALQMAYQELDEVVGRERRVK 375
                    K+T + +I GG+++S  TLTWA+SLLLNN   L+ A  E+D  VGR+R V+
Sbjct: 312 LQYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVE 371

Query: 376 ENDITKLVYLQAIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPNIWKIQTDP 435
           ++DI  LVYLQAIIKET+R+YPAGPLLGPRE  +DC VAGY+VP GT+LI N+WKIQ DP
Sbjct: 372 DSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDP 431

Query: 436 RVWPDPFEFKPERFLTTH-KDVDLKGNNFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQ 485
           +V+ +P EF+PERF+T   K+ D++G NFEL+PFGSGRR CPG +  +Q++H  LA FL 
Sbjct: 432 KVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLH 491

BLAST of Lag0036897 vs. ExPASy Swiss-Prot
Match: O49394 (Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=1 SV=2)

HSP 1 Score: 412.1 bits (1058), Expect = 8.5e-114
Identity = 223/520 (42.88%), Postives = 315/520 (60.58%), Query Frame = 0

Query: 16  LIIVLFISCYCLSRKWQKRGVCEPK--KAPSPPGAWPIIGHLHLLN-NGKLPHHVLGAMA 75
           +++ +FI+ +  S+K        PK  KAP+P GAWPIIGHLHLL+   +L +  LG MA
Sbjct: 12  ILVFVFIALFKKSKK--------PKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLGKMA 71

Query: 76  DKYGPIFRLQLGSRSALVVSNWEMAKASMCVNDAAAASRPELSVSKHFSYDFAMFGLASY 135
           D+YGP   L+LGS    VVS++E+AK    VND A ASRP  + +KH  YD A+FG A Y
Sbjct: 72  DQYGPAMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAPY 131

Query: 136 SPFWRDMRKITHLELLSNPRVDQVKSIMFGELDTSLTELNATWAAKKNEWGQISLEMKRW 195
           S FWR+MRKI  LELLSN R+  +K +   E+   + +L + W  KK     + +++K W
Sbjct: 132 SAFWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLW-VKKGGSEPVMVDLKSW 191

Query: 196 FGDVIVNMLLKVIIGKRCVGPNAMGGE--EEAKELQMAIRDSFHLMGEGLLRDYIPMVAK 255
             D+ +NM+++++ GKR  G  ++  E  EEA++ +  + + FHL+G   + D  P +  
Sbjct: 192 LEDMSLNMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLGW 251

Query: 256 LGFDGQVKAMEKIAERIDAIMG------------VGSKNT-------------------- 315
             F G  K M++    +D I+              G+K+                     
Sbjct: 252 FDFQGHEKEMKQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKFSHL 311

Query: 316 ------------INMIAGGTESSTVTLTWAMSLLLNNPRALQMAYQELDEVVGRERRVKE 375
                       + +I GG+E+S  TLTWA+SLLLNN   L+ A  E+D  VGR+R V++
Sbjct: 312 QHDAITSIKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVED 371

Query: 376 NDITKLVYLQAIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPNIWKIQTDPR 435
           +DI  LVY+QAIIKET+R+YPAGPLLG RE  +DC VAGY V +GT+++ N+WKIQ DPR
Sbjct: 372 SDIENLVYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPR 431

Query: 436 VWPDPFEFKPERFLTTH-KDVDLKGNNFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQS 486
           V+ +P EF+PERF+T   K+ D++G NFEL+PFGSGRR CPG +  +Q++H  LA FLQS
Sbjct: 432 VYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQS 491

BLAST of Lag0036897 vs. ExPASy TrEMBL
Match: A0A6J1IND1 (cytochrome P450 82A3-like OS=Cucurbita maxima OX=3661 GN=LOC111476807 PE=3 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 3.2e-233
Identity = 408/532 (76.69%), Postives = 445/532 (83.65%), Query Frame = 0

Query: 1   MEFLLPSLNPLSIATLIIVLFISCYCLSRKWQKRGVCEPKKAPSPPGAWPIIGHLHLLNN 60
           MEF LP LNPLSI  L I+   SCY LSRKW KRGVCEPKKAPSPPGAWPIIGHLHLLN+
Sbjct: 1   MEFPLPYLNPLSIILLFII--SSCY-LSRKWVKRGVCEPKKAPSPPGAWPIIGHLHLLNS 60

Query: 61  GKLPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKASMCVNDAAAASRPELSVSKHF 120
           GKLPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAK SMC+ND AAASRPELSVSK+F
Sbjct: 61  GKLPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKESMCINDGAAASRPELSVSKNF 120

Query: 121 SYDFAMFGLASYSPFWRDMRKITHLELLSNPRVDQVKSIMFGELDTSLTELNATWAAKKN 180
           SY FAMFGLASY  +WRDMRKITHL+LLSNPRVDQVKS+M  E+ TS+TEL+A WA++KN
Sbjct: 121 SYHFAMFGLASYGTYWRDMRKITHLQLLSNPRVDQVKSMMLSEMATSVTELHARWASEKN 180

Query: 181 EWGQISLEMKRWFGDVIVNMLLKVIIGKRCVGPNAMGGEEEAKELQMAIRDSFHLMGEGL 240
           + G I +EMKRWFGDV VNMLLKVIIGKRCVGPNA G E+EA +LQMAIR SFHLMGEGL
Sbjct: 181 QLGHIKVEMKRWFGDVTVNMLLKVIIGKRCVGPNAKGDEKEAIDLQMAIRKSFHLMGEGL 240

Query: 241 LRDYIPMVAKLGFDGQVKAMEKIAERIDAIMGV--------GSKN--------------- 300
           LRDYIP++A +GFD  VKAMEKIA+RID+I+          G KN               
Sbjct: 241 LRDYIPILANMGFDRHVKAMEKIAKRIDSILQCWLEEHRRNGDKNQSLDTKDGDFMDSLL 300

Query: 301 ---------------------TINMIAGGTESSTVTLTWAMSLLLNNPRALQMAYQELDE 360
                                T+NMIAGGTESSTVTLTWA+SLL+NNPRAL+ AY+ELD+
Sbjct: 301 SLCRANELPSHFDHDTIVKATTLNMIAGGTESSTVTLTWALSLLVNNPRALEKAYEELDQ 360

Query: 361 VVGRERRVKENDITKLVYLQAIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIP 420
           VVGR+RRVKE+DITKLVYLQA++KETMR+YPAGPLLGPREFYKDC VAGYFVPKGTQLIP
Sbjct: 361 VVGRDRRVKESDITKLVYLQAVVKETMRLYPAGPLLGPREFYKDCFVAGYFVPKGTQLIP 420

Query: 421 NIWKIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPGVAFGLQMVH 480
           NIWKIQTDPRVW DPFEFKPERFLTTH DVDLKGNNFELIPFGSGRRGCP VAFGLQMVH
Sbjct: 421 NIWKIQTDPRVWADPFEFKPERFLTTHCDVDLKGNNFELIPFGSGRRGCPAVAFGLQMVH 480

Query: 481 FTLAGFLQSFDVKSRTGEPVDMAEHFGMANEKVVPLNVLVTPRLPSHLHGPM 489
           F LAGFL SF VK+ T +PVDMAE+FGMANEKVVPLNVLVTPRLPSHLHGP+
Sbjct: 481 FALAGFLHSFTVKTPTPQPVDMAENFGMANEKVVPLNVLVTPRLPSHLHGPI 529

BLAST of Lag0036897 vs. ExPASy TrEMBL
Match: A0A6J1FBC6 (cytochrome P450 82A3-like OS=Cucurbita moschata OX=3662 GN=LOC111442561 PE=3 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 1.7e-226
Identity = 396/532 (74.44%), Postives = 433/532 (81.39%), Query Frame = 0

Query: 1   MEFLLPSLNPLSIATLIIVLFISCYCLSRKWQKRGVCEPKKAPSPPGAWPIIGHLHLLNN 60
           MEF LP LNPLSI  L I+   SCY LSRKW KRGVCE KKAPSPPGAWPIIGHLHLLNN
Sbjct: 1   MEFPLPYLNPLSIILLFII--SSCY-LSRKWVKRGVCEAKKAPSPPGAWPIIGHLHLLNN 60

Query: 61  GKLPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKASMCVNDAAAASRPELSVSKHF 120
           GKLPH VLGAMADKYGPIF LQLGSR ALVVSNWEMAK SMC+ND AAASRPELSVSK+F
Sbjct: 61  GKLPHRVLGAMADKYGPIFHLQLGSRPALVVSNWEMAKESMCINDGAAASRPELSVSKNF 120

Query: 121 SYDFAMFGLASYSPFWRDMRKITHLELLSNPRVDQVKSIMFGELDTSLTELNATWAAKKN 180
           SY FAMFGLASYSP+WRDMRKI+HL LLSN RVDQ KS+M  E+ TS+TEL+  WA++KN
Sbjct: 121 SYHFAMFGLASYSPYWRDMRKISHLHLLSNGRVDQAKSMMLSEMATSVTELHTRWASEKN 180

Query: 181 EWGQISLEMKRWFGDVIVNMLLKVIIGKRCVGPNAMGGEEEAKELQMAIRDSFHLMGEGL 240
           + G I +EMKRWFGDV +NMLLKVIIGKRCVGPNA G E+EA +LQMAIR SFHLMGEG+
Sbjct: 181 QLGHIKVEMKRWFGDVTLNMLLKVIIGKRCVGPNAKGDEKEAIDLQMAIRKSFHLMGEGM 240

Query: 241 LRDYIPMVAKLGFDGQVKAMEKIAERIDAIM----------------------------- 300
           LRDYIP++A LGFD  VKAMEKIA RID+I+                             
Sbjct: 241 LRDYIPILANLGFDRHVKAMEKIAIRIDSILQGWLQEHRRNKAKNQSLDTKDGDFMDSLL 300

Query: 301 ---------------GVGSKNTINMIAGGTESSTVTLTWAMSLLLNNPRALQMAYQELDE 360
                           +     +NMIAGGTESS+VTLTWA+SLL+NNP AL+ AY+ELD+
Sbjct: 301 SLCRSNELPSQFDHDTIVKATILNMIAGGTESSSVTLTWALSLLVNNPGALEKAYEELDQ 360

Query: 361 VVGRERRVKENDITKLVYLQAIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIP 420
           VVGR+RRV E+DITKLVYLQA++KETMR+YPAGPLLGPREFYKDC VAGYFVPKGTQLIP
Sbjct: 361 VVGRDRRVNESDITKLVYLQAVVKETMRLYPAGPLLGPREFYKDCFVAGYFVPKGTQLIP 420

Query: 421 NIWKIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPGVAFGLQMVH 480
           NIWKIQTDPRVWPDPFEFKPERFLTTH DVDLKGNNFELIPFGSGRRGCP VAFGLQMVH
Sbjct: 421 NIWKIQTDPRVWPDPFEFKPERFLTTHCDVDLKGNNFELIPFGSGRRGCPAVAFGLQMVH 480

Query: 481 FTLAGFLQSFDVKSRTGEPVDMAEHFGMANEKVVPLNVLVTPRLPSHLHGPM 489
           F LAG L SF VK+ T +PVDMAEHFGMANEKVVPLNVLVTPRLPSHLHGP+
Sbjct: 481 FALAGLLHSFTVKTPTPQPVDMAEHFGMANEKVVPLNVLVTPRLPSHLHGPI 529

BLAST of Lag0036897 vs. ExPASy TrEMBL
Match: A0A6J1FCU2 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442946 PE=3 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 6.5e-226
Identity = 393/532 (73.87%), Postives = 435/532 (81.77%), Query Frame = 0

Query: 1   MEFLLPSLNPLSIATLIIVLFISCYCLSRKWQKRGVCEPKKAPSPPGAWPIIGHLHLLNN 60
           MEF LP LNPLSI  L I+   SCY LSRKW KRGVCE KK PSPPGAWPIIGHLHLLNN
Sbjct: 1   MEFPLPYLNPLSIILLFII--SSCY-LSRKWVKRGVCEAKKVPSPPGAWPIIGHLHLLNN 60

Query: 61  GKLPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKASMCVNDAAAASRPELSVSKHF 120
           GKLPH VLGAMADKYGPIF LQ+GSR ALVVSNWEMAK SMC+ND AAASRPELSVSK+F
Sbjct: 61  GKLPHRVLGAMADKYGPIFHLQIGSRPALVVSNWEMAKESMCINDGAAASRPELSVSKNF 120

Query: 121 SYDFAMFGLASYSPFWRDMRKITHLELLSNPRVDQVKSIMFGELDTSLTELNATWAAKKN 180
           SY FAMFGLASYSP+WRDMRKI+HL LLSN RVDQVKS+M  E+ TS+TEL+A WA++KN
Sbjct: 121 SYHFAMFGLASYSPYWRDMRKISHLHLLSNRRVDQVKSMMLSEMATSVTELHARWASEKN 180

Query: 181 EWGQISLEMKRWFGDVIVNMLLKVIIGKRCVGPNAMGGEEEAKELQMAIRDSFHLMGEGL 240
           + G I++EMKRWFGDVI NMLLKVIIGKRCVGPNA G E+EA +LQMAI  SFHLMGEG+
Sbjct: 181 QMGHINVEMKRWFGDVIANMLLKVIIGKRCVGPNAKGDEKEAIDLQMAIGKSFHLMGEGM 240

Query: 241 LRDYIPMVAKLGFDGQVKAMEKIAERIDAIM----------------------------- 300
           LRDYIP++A LGFD   KAMEKIA RID+I+                             
Sbjct: 241 LRDYIPILANLGFDRHSKAMEKIATRIDSILQGWLQEHRRNRDKNQSLDTKDGDFMDSLL 300

Query: 301 ---------------GVGSKNTINMIAGGTESSTVTLTWAMSLLLNNPRALQMAYQELDE 360
                           +    T+NMIAGGTE+STVTLTWA+SLL+NNP AL+ AY+ELD+
Sbjct: 301 SLCRTNELPRHFDHDTIVKATTLNMIAGGTETSTVTLTWALSLLVNNPGALEKAYEELDQ 360

Query: 361 VVGRERRVKENDITKLVYLQAIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIP 420
           VVGR+RRV E+DITKLVYLQA++KETMR+YPAGPLLGPREFYKDC VAGYFVPKGTQLIP
Sbjct: 361 VVGRDRRVNESDITKLVYLQAVVKETMRLYPAGPLLGPREFYKDCFVAGYFVPKGTQLIP 420

Query: 421 NIWKIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPGVAFGLQMVH 480
           NIWKIQTDPRVWPDPFEFKPERFLTTH DVDLKGNNFELIPFGSGRRGCPG+AFGLQMVH
Sbjct: 421 NIWKIQTDPRVWPDPFEFKPERFLTTHCDVDLKGNNFELIPFGSGRRGCPGIAFGLQMVH 480

Query: 481 FTLAGFLQSFDVKSRTGEPVDMAEHFGMANEKVVPLNVLVTPRLPSHLHGPM 489
           F LAGFL SF+VK+ T + +DMAE FGMANEK+VPLNVLVTPRLPSHLHGP+
Sbjct: 481 FALAGFLHSFNVKTPTPQSIDMAEGFGMANEKLVPLNVLVTPRLPSHLHGPI 529

BLAST of Lag0036897 vs. ExPASy TrEMBL
Match: A0A6J1D746 (cytochrome P450 82A3-like OS=Momordica charantia OX=3673 GN=LOC111017857 PE=3 SV=1)

HSP 1 Score: 775.0 bits (2000), Expect = 1.8e-220
Identity = 380/518 (73.36%), Postives = 428/518 (82.63%), Query Frame = 0

Query: 10  PLSIATLIIVLFISCYCLSRKWQKRGVCEPKKAPSPPGAWPIIGHLHLLNNGKLPHHVLG 69
           P+ IA + ++  +  Y L RK Q     + K APSPPGAWP+IGHLHLL+N KLPHH LG
Sbjct: 16  PICIAAIFLIFIVCYYYLCRKRQD----QRKLAPSPPGAWPVIGHLHLLHNAKLPHHTLG 75

Query: 70  AMADKYGPIFRLQLGSRSALVVSNWEMAKASMCVNDAAAASRPELSVSKHFSYDFAMFGL 129
           AMADKYGPIFRLQLGSRSALVVSNWEMAK SMC+NDAAAASRPELSVSKHFSYDFAMFGL
Sbjct: 76  AMADKYGPIFRLQLGSRSALVVSNWEMAKESMCINDAAAASRPELSVSKHFSYDFAMFGL 135

Query: 130 ASYSPFWRDMRKITHLELLSNPRVDQVKSIMFGELDTSLTELNATWAAKKNEWGQISLEM 189
           ASY P+WR+MRKITHLELLSNPRV +VK+IMFGE+D SLTEL+A W  ++NE GQI +E+
Sbjct: 136 ASYGPYWREMRKITHLELLSNPRVQEVKNIMFGEVDMSLTELHAKWVVQRNESGQIPVEL 195

Query: 190 KRWFGDVIVNMLLKVIIGKRCVGPNAMGGEEEAKELQMAIRDSFHLMGEGLLRDYIPMVA 249
           K WFGDV++NMLLK+IIGKRCVGPNA+GGE EAKELQMAIR+SF LMG+G+LRD+IP+VA
Sbjct: 196 KGWFGDVVMNMLLKIIIGKRCVGPNALGGEAEAKELQMAIRESFRLMGQGILRDFIPVVA 255

Query: 250 KLGFDGQVKAMEKIAERIDAIM-------------GVGSKN------------------- 309
           +LGFDGQVKAMEKIA+RIDAI+             G+  KN                   
Sbjct: 256 QLGFDGQVKAMEKIAKRIDAILEGWLQDHKKNRAYGLAKKNGDFMVSLLSLYDANQLPSQ 315

Query: 310 ----------TINMIAGGTESSTVTLTWAMSLLLNNPRALQMAYQELDEVVGRERRVKEN 369
                     T+NMIAGGTESSTVTLTWAMSLLLNNP AL+ AYQELD VVGR+RRV E+
Sbjct: 316 YNGDIITKATTLNMIAGGTESSTVTLTWAMSLLLNNPHALEKAYQELDAVVGRDRRVNES 375

Query: 370 DITKLVYLQAIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPNIWKIQTDPRV 429
           DI+KL YLQAI+KETMR+YPAGPLLGPREFYKDCVV GYFVPKGTQLIPNIWKIQ DPRV
Sbjct: 376 DISKLSYLQAIVKETMRLYPAGPLLGPREFYKDCVVGGYFVPKGTQLIPNIWKIQVDPRV 435

Query: 430 WPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFD 486
           WPDP +FKPERFLTTHK+VD+KGN+FELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFD
Sbjct: 436 WPDPLKFKPERFLTTHKEVDVKGNDFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFD 495

BLAST of Lag0036897 vs. ExPASy TrEMBL
Match: A0A0A0LD43 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852590 PE=3 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 2.4e-220
Identity = 374/528 (70.83%), Postives = 431/528 (81.63%), Query Frame = 0

Query: 1   MEFLLPSLNPLSIATLIIVLFISCYCLSRKWQKRGVCEPKKAPSPPGAWPIIGHLHLLNN 60
           +EF  P  NP SI  L+ + FISCY L +KWQ+    EPK+AP+PPGAWPIIGHLH+L+N
Sbjct: 2   VEFQFPFFNPKSITMLVFLFFISCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLHN 61

Query: 61  GKLPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKASMCVNDAAAASRPELSVSKHF 120
            KLPHH LGAMADKYGP+FRLQLGSRSALVVS+WEMAK SMCVNDAAAASRP +S +KHF
Sbjct: 62  VKLPHHALGAMADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKHF 121

Query: 121 SYDFAMFGLASYSPFWRDMRKITHLELLSNPRVDQVKSIMFGELDTSLTELNATWAAKKN 180
           SYDFA FGLA YSP+WR++RK+TH+ELLSNPRVDQ K+ MFGE+ TSL EL+ TWAA+K+
Sbjct: 122 SYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQKD 181

Query: 181 EWGQISLEMKRWFGDVIVNMLLKVIIGKRCVGPNAMGGEEEAKELQMAIRDSFHLMGEGL 240
             GQ+ +EMKRWFGDVIVNMLLK+IIGKRCVGPNA GGE++AK+ Q+AIRDSFHLMG+GL
Sbjct: 182 GSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGL 241

Query: 241 LRDYIPMVAKLGFDGQVKAMEKIAERIDAIMG--------------VGSKN--------- 300
           LRDYIP++ +LGF+GQVK ME IA R D ++                G K+         
Sbjct: 242 LRDYIPLIGRLGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALVS 301

Query: 301 --------------------TINMIAGGTESSTVTLTWAMSLLLNNPRALQMAYQELDEV 360
                               T+NM+AGGTES+TVTLTWAMSLL+NNP  L+ A QELD V
Sbjct: 302 LYDGKEIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDTV 361

Query: 361 VGRERRVKENDITKLVYLQAIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPN 420
           VGR+R++KE+DI  LVYL++IIKETMRMYPAGPLLGPREFYKDC+VAGYFVPKGTQLIPN
Sbjct: 362 VGRDRQLKESDIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIPN 421

Query: 421 IWKIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPGVAFGLQMVHF 480
           IWKIQTDPRVWPDPFEFKPERFLTTHK+VDLKGNNFELIPFGSGRRGCPG+AFGLQMVHF
Sbjct: 422 IWKIQTDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVHF 481

Query: 481 TLAGFLQSFDVKSRTGEPVDMAEHFGMANEKVVPLNVLVTPRLPSHLH 486
            LAGFL SFDVK+ T EP+DM+E+FGMANEKVVPLNV VT RLPSHL+
Sbjct: 482 ALAGFLHSFDVKNPTKEPIDMSENFGMANEKVVPLNVSVTSRLPSHLY 529

BLAST of Lag0036897 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 414.5 bits (1064), Expect = 1.2e-115
Identity = 225/520 (43.27%), Postives = 312/520 (60.00%), Query Frame = 0

Query: 16  LIIVLFISCYCLSRKWQKRGVCEPK--KAPSPPGAWPIIGHLHLL-NNGKLPHHVLGAMA 75
           +++ +FI+ +  S+K        PK  KAP+P GAWPIIGHLHLL    +L +  LG MA
Sbjct: 12  ILVFVFIALFKKSKK--------PKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMA 71

Query: 76  DKYGPIFRLQLGSRSALVVSNWEMAKASMCVNDAAAASRPELSVSKHFSYDFAMFGLASY 135
           D YGP   LQLGS  A VVS++E+AK    VND A ASRP  + +KH  Y+FA+FG A Y
Sbjct: 72  DHYGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAPY 131

Query: 136 SPFWRDMRKITHLELLSNPRVDQVKSIMFGELDTSLTELNATWAAKKNEWGQISLEMKRW 195
           S FWR+MRKI  +ELLSN R+  +K +   E+   + +L + W  K      + +++K W
Sbjct: 132 SAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLW-FKNGGTKPVMVDLKSW 191

Query: 196 FGDVIVNMLLKVIIGKRCVGPN---AMGGEEEAKELQMAIRDSFHLMGEGLLRDYIPMVA 255
             D+ +NM+++++ GKR  G     +    EEA + + AI   FHL+G   + D  P ++
Sbjct: 192 LEDMTLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLS 251

Query: 256 KLGFDGQVKAMEKIAERIDAIM-------------------------------------- 315
                G  K M++    +D I+                                      
Sbjct: 252 FFDLQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAEQGKLSH 311

Query: 316 -----GVGSKNT-INMIAGGTESSTVTLTWAMSLLLNNPRALQMAYQELDEVVGRERRVK 375
                    K+T + +I GG+++S  TLTWA+SLLLNN   L+ A  E+D  VGR+R V+
Sbjct: 312 LQYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVE 371

Query: 376 ENDITKLVYLQAIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPNIWKIQTDP 435
           ++DI  LVYLQAIIKET+R+YPAGPLLGPRE  +DC VAGY+VP GT+LI N+WKIQ DP
Sbjct: 372 DSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDP 431

Query: 436 RVWPDPFEFKPERFLTTH-KDVDLKGNNFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQ 485
           +V+ +P EF+PERF+T   K+ D++G NFEL+PFGSGRR CPG +  +Q++H  LA FL 
Sbjct: 432 KVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLH 491

BLAST of Lag0036897 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 412.1 bits (1058), Expect = 6.0e-115
Identity = 223/520 (42.88%), Postives = 315/520 (60.58%), Query Frame = 0

Query: 16  LIIVLFISCYCLSRKWQKRGVCEPK--KAPSPPGAWPIIGHLHLLN-NGKLPHHVLGAMA 75
           +++ +FI+ +  S+K        PK  KAP+P GAWPIIGHLHLL+   +L +  LG MA
Sbjct: 12  ILVFVFIALFKKSKK--------PKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLGKMA 71

Query: 76  DKYGPIFRLQLGSRSALVVSNWEMAKASMCVNDAAAASRPELSVSKHFSYDFAMFGLASY 135
           D+YGP   L+LGS    VVS++E+AK    VND A ASRP  + +KH  YD A+FG A Y
Sbjct: 72  DQYGPAMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAPY 131

Query: 136 SPFWRDMRKITHLELLSNPRVDQVKSIMFGELDTSLTELNATWAAKKNEWGQISLEMKRW 195
           S FWR+MRKI  LELLSN R+  +K +   E+   + +L + W  KK     + +++K W
Sbjct: 132 SAFWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLW-VKKGGSEPVMVDLKSW 191

Query: 196 FGDVIVNMLLKVIIGKRCVGPNAMGGE--EEAKELQMAIRDSFHLMGEGLLRDYIPMVAK 255
             D+ +NM+++++ GKR  G  ++  E  EEA++ +  + + FHL+G   + D  P +  
Sbjct: 192 LEDMSLNMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLGW 251

Query: 256 LGFDGQVKAMEKIAERIDAIMG------------VGSKNT-------------------- 315
             F G  K M++    +D I+              G+K+                     
Sbjct: 252 FDFQGHEKEMKQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKFSHL 311

Query: 316 ------------INMIAGGTESSTVTLTWAMSLLLNNPRALQMAYQELDEVVGRERRVKE 375
                       + +I GG+E+S  TLTWA+SLLLNN   L+ A  E+D  VGR+R V++
Sbjct: 312 QHDAITSIKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVED 371

Query: 376 NDITKLVYLQAIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPNIWKIQTDPR 435
           +DI  LVY+QAIIKET+R+YPAGPLLG RE  +DC VAGY V +GT+++ N+WKIQ DPR
Sbjct: 372 SDIENLVYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPR 431

Query: 436 VWPDPFEFKPERFLTTH-KDVDLKGNNFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQS 486
           V+ +P EF+PERF+T   K+ D++G NFEL+PFGSGRR CPG +  +Q++H  LA FLQS
Sbjct: 432 VYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQS 491

BLAST of Lag0036897 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 368.2 bits (944), Expect = 1.0e-101
Identity = 213/521 (40.88%), Postives = 294/521 (56.43%), Query Frame = 0

Query: 15  TLIIVLFISCYCLSRKWQKRGVCEPK--KAPSPPGAWPIIGHLHLL-NNGKLPHHVLGAM 74
           ++++ +FI+ +  S+K        PK  KAP+P GAWPIIGHLHLL    +L +  LG M
Sbjct: 11  SILVFVFIALFKKSKK--------PKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKM 70

Query: 75  ADKYGPIFRLQLGSRSALVVSNWEMAKASMCVNDAAAASRPELSVSKHFSYDFAMFGLAS 134
           AD YGP   L+LGS    V S++E+AK    VND A AS    + +KH  Y         
Sbjct: 71  ADHYGPAMSLRLGSSETFVGSSFEVAKDCFTVNDKALASL-MTAAAKHMGY--------- 130

Query: 135 YSPFWRDMRKITHLELLSNPRVDQVKSIMFGELDTSLTELNATWAAKKNEWGQISLEMKR 194
              FW +MRKI  +ELLSN R+  + ++   E+   + +L + W  KK     + +++K 
Sbjct: 131 --VFWLEMRKIAMIELLSNRRLQMLNNVRVSEISMGVKDLYSLW-VKKGGSEPVMVDLKS 190

Query: 195 WFGDVIVNMLLKVIIGKRCVGPNAMGGE---EEAKELQMAIRDSFHLMGEGLLRDYIPMV 254
           W  D+I NM+++++ GKR  G          EEA++ +  I   FHL+G   + D  P +
Sbjct: 191 WLEDMIANMIMRMVAGKRYFGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFPKL 250

Query: 255 AKLGFDGQVKAMEKIAERIDAIMG------------VGSKNT------------------ 314
             L   G  K M++    +D I+              G+K+                   
Sbjct: 251 GWLDLQGHEKEMKQTRRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKLS 310

Query: 315 --------------INMIAGGTESSTVTLTWAMSLLLNNPRALQMAYQELDEVVGRERRV 374
                         + +I GG+E+S  TLTWA+SLLLNN   L+    E+D  VGR+R V
Sbjct: 311 HLQYDANTCIKTTCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVGRDRNV 370

Query: 375 KENDITKLVYLQAIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPNIWKIQTD 434
           +++DI  LVYLQAIIKET+R+YPA PLLG RE  +DC VAGY VP GT+LI N+WKIQ D
Sbjct: 371 EDSDIKNLVYLQAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVWKIQRD 430

Query: 435 PRVWPDPFEFKPERFLTTH-KDVDLKGNNFELIPFGSGRRGCPGVAFGLQMVHFTLAGFL 485
           P+V+ +P EF+PERF+T   KD D++G NFEL+PFGSGRR CPG +  +QM+H  LA FL
Sbjct: 431 PKVYMEPNEFRPERFITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGLARFL 490

BLAST of Lag0036897 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 324.7 bits (831), Expect = 1.3e-88
Identity = 186/512 (36.33%), Postives = 270/512 (52.73%), Query Frame = 0

Query: 16  LIIVLFISCYCLSRKWQKRGVCEPKKAPSPPGAWPIIGHLHLLNNGKLPHHVLGAMADKY 75
           L+  LFI    +  K + R   +   AP  PGAWP++GHLHL +     H   GAMAD Y
Sbjct: 8   LLSALFIFPVLILIKSRLRPKNKKSTAPMVPGAWPLLGHLHLFDTVNPTHVTFGAMADVY 67

Query: 76  GPIFRLQLGSRSALVVSNWEMAKASMCVNDAAAASRPELSVSKHFSYDFAMFGLASYSPF 135
           GP+F  +LGS   +++++ E+AK    V+D     RPEL+ SK   Y+ +    + Y  +
Sbjct: 68  GPVFMAKLGSIKVMIINSKEVAKEIYTVHD-KLLERPELTASKLLGYNDSFLTFSPYGLY 127

Query: 136 WRDMRKITHLELLSNPRVDQVKSIMFGELDTSLTELNATWAAKKNEWGQISLEMKRWFGD 195
           WR++RKI   EL S   VD        E D +   L   W  +      + ++MK+ F D
Sbjct: 128 WREIRKIAVSELFSTSGVDMHMVSRAREADLAFRALYVRWEKRGKPKEGVLVDMKQEFID 187

Query: 196 VIVNMLLKVIIGKRCVGPNAMGGEEEAKELQMAIRDSFHLMGEGLLRDYIPMV------A 255
           +  N+ L ++ GKR  G N     +EA+     IR+        LL D  P++       
Sbjct: 188 LTANISLMMVSGKRYFGENPNCEVKEARRCGKLIREFLDYFALFLLSDVAPVLGFLDWKT 247

Query: 256 KLGFDGQVKAMEKIAE----------------------------RIDAIMGVGSKNT--- 315
           K G     K ++K+AE                              D I G+   +T   
Sbjct: 248 KRGMKRTAKGLDKVAEGWIEEHKNKRSDHGRSENDYLDILIKILGQDKIPGLSDTHTKIK 307

Query: 316 ---INMIAGGTESSTVTLTWAMSLLLNNPRALQMAYQELDEVVGRERRVKENDITKLVYL 375
              +N++  G+E++ V L WA+SLLLNNP  L+ A +ELD  +G+ER V+E DI  LVYL
Sbjct: 308 ALCLNLVLAGSETAIVVLVWAVSLLLNNPHVLRKAQEELDSKIGKERVVEELDIKDLVYL 367

Query: 376 QAIIKETMRMYPAGPLLGPREFYKDCVVA--GYFVPKGTQLIPNIWKIQTDPRVWPDPFE 435
           QAI+KET R+YP  PL+  R   +D  +A     VP GTQL+ + WKI  DP VW +P +
Sbjct: 368 QAIVKETFRLYPPVPLVAYRAVVEDFDIAFCKCHVPAGTQLMVSAWKIHRDPNVWSNPEQ 427

Query: 436 FKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFDVKSRTG 486
           F+PERFLT+++++D+ G +++  PFG GRR CP +  G++MVH+ L  FL SFD+   + 
Sbjct: 428 FEPERFLTSNRELDVGGQSYKFFPFGLGRRSCPAIPLGMRMVHYLLVRFLHSFDLARPSS 487

BLAST of Lag0036897 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 323.6 bits (828), Expect = 2.8e-88
Identity = 197/526 (37.45%), Postives = 287/526 (54.56%), Query Frame = 0

Query: 1   MEFLLPSLNPLSIATLIIVLFISCYCLSRKWQKRGVCEPKKAPSPPGAWPIIGHLHLLNN 60
           M FL  +L  LS+ +L +V+F   Y   RK   R   +    P P GA P+ GHLHLL  
Sbjct: 1   MTFLFSTLQ-LSLFSLALVIF--GYIFLRKQLSRCEVDSSTIPEPLGALPLFGHLHLLRG 60

Query: 61  GKLPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKASMCVNDAAAASRPELSVSKHF 120
            KL    L AM+ K+GPIF L+LG    +V S+ +  K     ND A A+RP ++  ++ 
Sbjct: 61  KKLLCKKLAAMSQKHGPIFSLKLGFYRLVVASDPKTVKDCFTTNDLATATRPNIAFGRYV 120

Query: 121 SYDFAMFGLASYSPFWRDMRKITHLELLSNPRVDQVKSIMFGELDTSLTELNATWAAKKN 180
            Y+ A   LA Y  +WR++RKI  + L SN  ++ +  I   E++T +  L       K 
Sbjct: 121 GYNNASLTLAPYGDYWRELRKIVTVHLFSNHSIEMLGHIRSSEVNTLIKHL------YKG 180

Query: 181 EWGQISLEMKRWFGDVIVNMLLKVIIGKRCVGPNAMGGEEEAKELQMAIRDSFHLMGEGL 240
             G   +++   F  +  N++L+ ++GKR         E   KE   A++   +L    +
Sbjct: 181 NGGTSIVKIDMLFEFLTFNIILRKMVGKRIGFGEVNSDEWRYKE---ALKHCEYLAVIPM 240

Query: 241 LRDYIPMVAKLGF--DGQVKAM----------------------EKIAER--IDAIMGVG 300
           + D IP +  L F  + Q+K +                      EK  ER  +D ++ + 
Sbjct: 241 IGDVIPWLGWLDFAKNSQMKRLFKELDSVNTKWLHEHLKKRSRNEKDQERTIMDLLLDIL 300

Query: 301 SKNTI---------------NMIAGGTESSTVTLTWAMSLLLNNPRALQMAYQELDEVVG 360
            ++ +                +   G++S+++TLTWA+SLLLNNP AL+ A +E+D  VG
Sbjct: 301 PEDIVISGHVRDVIVKATILALTLTGSDSTSITLTWAVSLLLNNPAALEAAQEEIDNSVG 360

Query: 361 RERRVKENDITKLVYLQAIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPNIW 420
           + R ++E+DI  L YLQAI+KET R+YP  PL G RE  +DC V GY V KGT+L+ NIW
Sbjct: 361 KGRWIEESDIQNLKYLQAIVKETHRLYPPAPLTGIREAREDCFVGGYRVEKGTRLLVNIW 420

Query: 421 KIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPGVAFGLQMVHFTL 480
           K+  DP++WPDP  FKPERF+      +   +NFE IPFGSGRR CPGV  GL++VHF L
Sbjct: 421 KLHRDPKIWPDPKTFKPERFMEDKSQCE--KSNFEYIPFGSGRRSCPGVNLGLRVVHFVL 480

Query: 481 AGFLQSFDVKSRTGEPVDMAEHFGMANEKVVPLNVLVTPRLPSHLH 486
           A  LQ F++   + EP+DMAE  G+A  K+ P+ V+V PRL   L+
Sbjct: 481 ARLLQGFELHKVSDEPLDMAEGPGLALPKINPVEVVVMPRLDPKLY 512

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022976389.16.7e-23376.69cytochrome P450 82A3-like [Cucurbita maxima][more]
XP_023535454.17.6e-22975.19cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo][more]
XP_022935738.13.5e-22674.44cytochrome P450 82A3-like [Cucurbita moschata][more]
XP_022936278.11.3e-22573.87cytochrome P450 CYP82D47-like [Cucurbita moschata][more]
XP_022149464.13.8e-22073.36cytochrome P450 82A3-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
O498583.0e-12744.77Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1[more]
O498597.2e-12142.91Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1[more]
Q430686.3e-11740.67Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2[more]
Q9SZ461.7e-11443.27Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=... [more]
O493948.5e-11442.88Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1IND13.2e-23376.69cytochrome P450 82A3-like OS=Cucurbita maxima OX=3661 GN=LOC111476807 PE=3 SV=1[more]
A0A6J1FBC61.7e-22674.44cytochrome P450 82A3-like OS=Cucurbita moschata OX=3662 GN=LOC111442561 PE=3 SV=... [more]
A0A6J1FCU26.5e-22673.87cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442946 PE=3... [more]
A0A6J1D7461.8e-22073.36cytochrome P450 82A3-like OS=Momordica charantia OX=3673 GN=LOC111017857 PE=3 SV... [more]
A0A0A0LD432.4e-22070.83Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852590 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G31940.11.2e-11543.27cytochrome P450, family 82, subfamily C, polypeptide 4 [more]
AT4G31970.16.0e-11542.88cytochrome P450, family 82, subfamily C, polypeptide 2 [more]
AT4G31950.11.0e-10140.88cytochrome P450, family 82, subfamily C, polypeptide 3 [more]
AT2G25160.11.3e-8836.33cytochrome P450, family 82, subfamily F, polypeptide 1 [more]
AT3G25180.12.8e-8837.45cytochrome P450, family 82, subfamily G, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 72..91
score: 37.09
coord: 293..319
score: 34.33
coord: 377..401
score: 41.12
coord: 336..354
score: 36.6
coord: 425..448
score: 26.74
coord: 415..425
score: 53.79
IPR001128Cytochrome P450PRINTSPR00385P450coord: 284..301
score: 31.45
coord: 416..425
score: 57.4
coord: 337..348
score: 41.7
IPR001128Cytochrome P450PFAMPF00067p450coord: 43..268
e-value: 3.4E-19
score: 69.0
coord: 277..463
e-value: 4.8E-59
score: 200.3
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 35..272
e-value: 2.2E-32
score: 114.2
coord: 275..478
e-value: 2.7E-67
score: 229.4
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 43..479
NoneNo IPR availablePANTHERPTHR47947CYTOCHROME P450 82C3-RELATEDcoord: 40..271
coord: 277..484
NoneNo IPR availablePANTHERPTHR47947:SF26CYTOCHROME P450coord: 277..484
NoneNo IPR availablePANTHERPTHR47947:SF26CYTOCHROME P450coord: 40..271
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 418..427

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0036897.1Lag0036897.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098542 defense response to other organism
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen