Lag0036887 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0036887
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Cytochrome P450
Location	chr2: 1877529 .. 1878158 (-)
RNA-Seq Expression	Lag0036887
Synteny	Lag0036887

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGAGTATAATATTGGGTGCATTTGATACTACAACAATAACAATCATATGGGTTGTTTCATTACTTCTCAACAATGAAGAAGCACTCAGAAAAGTCCAACTTGAATTAGATGAACAAATTGGAAGAGAAAGACAAGTAAAAGAGTCTGATGTGAAAAACCTTCCATATCTCCAAGCTATTGTGAAGGAAACTCTACGGTTGTATCCTGCTGCGCCACTCTTAGTCCCTCATGAATCTACAGAAGATTGCACAACTGTTGGCTACCACATTCCGGCAGGGACACGCCTCATAATAAATGCTCAAAAGCTCCAAAGGGACCCTCTTGTTTGGGCGGATCCTTGTGAATTTCGACCCGAAAGATTTTTAACAACTCAAAAGAGTTTTGATGTTAGAGGACAAAGCCCCCAATTGATACCATTCGGAAATGGTCGAAGGATGTGTCCTGGGATCTCTTTCGCCCTCCAAATTATACATCTTACGCTTGCAAGCTTGCTTCATGGGTTTAAAATTGGTAGGCCATCAGAAGAACTACTCGACATGGAAGAGAGTATTGGATTGACCAGTGTTAGAAAATCTCCACTCGAAGTTGTTTTAATTCCACGCCTACCCGCCCAAGTTTATGGGTAA

mRNA sequence

ATGCAGAGTATAATATTGGGTGCATTTGATACTACAACAATAACAATCATATGGGTTGTTTCATTACTTCTCAACAATGAAGAAGCACTCAGAAAAGTCCAACTTGAATTAGATGAACAAATTGGAAGAGAAAGACAAGTAAAAGAGTCTGATGTGAAAAACCTTCCATATCTCCAAGCTATTGTGAAGGAAACTCTACGGTTGTATCCTGCTGCGCCACTCTTAGTCCCTCATGAATCTACAGAAGATTGCACAACTGTTGGCTACCACATTCCGGCAGGGACACGCCTCATAATAAATGCTCAAAAGCTCCAAAGGGACCCTCTTGTTTGGGCGGATCCTTGTGAATTTCGACCCGAAAGATTTTTAACAACTCAAAAGAGTTTTGATGTTAGAGGACAAAGCCCCCAATTGATACCATTCGGAAATGGTCGAAGGATGTGTCCTGGGATCTCTTTCGCCCTCCAAATTATACATCTTACGCTTGCAAGCTTGCTTCATGGGTTTAAAATTGGTAGGCCATCAGAAGAACTACTCGACATGGAAGAGAGTATTGGATTGACCAGTGTTAGAAAATCTCCACTCGAAGTTGTTTTAATTCCACGCCTACCCGCCCAAGTTTATGGGTAA

Coding sequence (CDS)

ATGCAGAGTATAATATTGGGTGCATTTGATACTACAACAATAACAATCATATGGGTTGTTTCATTACTTCTCAACAATGAAGAAGCACTCAGAAAAGTCCAACTTGAATTAGATGAACAAATTGGAAGAGAAAGACAAGTAAAAGAGTCTGATGTGAAAAACCTTCCATATCTCCAAGCTATTGTGAAGGAAACTCTACGGTTGTATCCTGCTGCGCCACTCTTAGTCCCTCATGAATCTACAGAAGATTGCACAACTGTTGGCTACCACATTCCGGCAGGGACACGCCTCATAATAAATGCTCAAAAGCTCCAAAGGGACCCTCTTGTTTGGGCGGATCCTTGTGAATTTCGACCCGAAAGATTTTTAACAACTCAAAAGAGTTTTGATGTTAGAGGACAAAGCCCCCAATTGATACCATTCGGAAATGGTCGAAGGATGTGTCCTGGGATCTCTTTCGCCCTCCAAATTATACATCTTACGCTTGCAAGCTTGCTTCATGGGTTTAAAATTGGTAGGCCATCAGAAGAACTACTCGACATGGAAGAGAGTATTGGATTGACCAGTGTTAGAAAATCTCCACTCGAAGTTGTTTTAATTCCACGCCTACCCGCCCAAGTTTATGGGTAA

Protein sequence

MQSIILGAFDTTTITIIWVVSLLLNNEEALRKVQLELDEQIGRERQVKESDVKNLPYLQAIVKETLRLYPAAPLLVPHESTEDCTTVGYHIPAGTRLIINAQKLQRDPLVWADPCEFRPERFLTTQKSFDVRGQSPQLIPFGNGRRMCPGISFALQIIHLTLASLLHGFKIGRPSEELLDMEESIGLTSVRKSPLEVVLIPRLPAQVYG

Homology

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BLAST of Lag0036887 vs. NCBI nr
Match: XP_022138438.1 (cytochrome P450 CYP82D47-like [Momordica charantia])

HSP 1 Score: 356.7 bits (914), Expect = 1.4e-94
Identity = 169/206 (82.04%), Postives = 189/206 (91.75%), Query Frame = 0


Query: 3   SIILGAFDTTTITIIWVVSLLLNNEEALRKVQLELDEQIGRERQVKESDVKNLPYLQAIV 62
           ++ILG FDTTT+T+ W +SLLLNN+EAL+K QLELDEQ+GRERQV ESDVKNL YL+AIV
Sbjct: 319 NMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRERQVNESDVKNLLYLRAIV 378

Query: 63  KETLRLYPAAPLLVPHESTEDCTTVGYHIPAGTRLIINAQKLQRDPLVWADPCEFRPERF 122
           KETLRLYP APLL PHES EDCT VGY+IP GTRLI+N +KLQRDP VW DPCEFRPERF
Sbjct: 379 KETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKLQRDPFVWEDPCEFRPERF 438

Query: 123 LTTQKSFDVRGQSPQLIPFGNGRRMCPGISFALQIIHLTLASLLHGFKIGRPSEELLDME 182
           LT+Q +FDVRGQSPQ IPFGNGRRMCPGISFALQ+IHLTLA+LLHGF+IGRPSEELLDME
Sbjct: 439 LTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLANLLHGFEIGRPSEELLDME 498

Query: 183 ESIGLTSVRKSPLEVVLIPRLPAQVY 209
           ES+GLTSVRK+PLE+VL PRLPAQVY
Sbjct: 499 ESVGLTSVRKTPLEIVLTPRLPAQVY 524

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BLAST of Lag0036887 vs. NCBI nr
Match: XP_022138580.1 (cytochrome P450 CYP82D47-like [Momordica charantia])

HSP 1 Score: 356.7 bits (914), Expect = 1.4e-94
Identity = 173/207 (83.57%), Postives = 189/207 (91.30%), Query Frame = 0


Query: 3   SIILGAFDTTTITIIWVVSLLLNNEEALRKVQLELDEQIGRERQVKESDVKNLPYLQAIV 62
           ++ILG FDTTT+T+ WV+SLLLNN+E L+K QLELDEQ+GR R+VKESDVKNL YLQAIV
Sbjct: 352 NMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKESDVKNLLYLQAIV 411

Query: 63  KETLRLYPAAPLLVPHESTEDCTTVGYHIPAGTRLIINAQKLQRDPLVWADPCEFRPERF 122
           KETLRLYPAAPLLVPHES EDCT  GYHIP GTRLI+N QKLQRDPLVW D CEFRPERF
Sbjct: 412 KETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDSCEFRPERF 471

Query: 123 LTTQKSFDVRGQSPQLIPFGNGRRMCPGISFALQIIHLTLASLLHGFKIGRPSEELLDME 182
           LT+QK+FDVRGQSPQLIPF NGRRMCPGISFALQ+IHLTLA+LLHGFKI RPSEE LDME
Sbjct: 472 LTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGFKIDRPSEEPLDME 531

Query: 183 ESIGLTSVRKSPLEVVLIPRLPAQVYG 210
           ES+GLTSVRK+PLEVVL P LPAQVYG
Sbjct: 532 ESVGLTSVRKAPLEVVLTPCLPAQVYG 558

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BLAST of Lag0036887 vs. NCBI nr
Match: XP_011652397.1 (cytochrome P450 CYP82D47 [Cucumis sativus] >KGN60056.2 hypothetical protein Csa_002536 [Cucumis sativus])

HSP 1 Score: 348.6 bits (893), Expect = 3.8e-92
Identity = 165/207 (79.71%), Postives = 189/207 (91.30%), Query Frame = 0


Query: 3   SIILGAFDTTTITIIWVVSLLLNNEEALRKVQLELDEQIGRERQVKESDVKNLPYLQAIV 62
           ++ILG FDTTT+T+ W + LLLNN+EAL+K Q+ELDEQ+GRERQVKE+D+KNLPYLQAIV
Sbjct: 312 NMILGGFDTTTVTMTWALCLLLNNKEALKKAQVELDEQVGRERQVKETDLKNLPYLQAIV 371

Query: 63  KETLRLYPAAPLLVPHESTEDCTTVGYHIPAGTRLIINAQKLQRDPLVWADPCEFRPERF 122
           KETLRLYPAAPLLVPHES EDCT  GYHIP GTRLI+N QKLQRDPLVW DP EFRPERF
Sbjct: 372 KETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQKLQRDPLVWEDPFEFRPERF 431

Query: 123 LTTQKSFDVRGQSPQLIPFGNGRRMCPGISFALQIIHLTLASLLHGFKIGRPSEELLDME 182
           LT+QK+FDVRGQ+PQ IPFGNGRRMCP ISFALQII+LTL++ LHGF+I RPSEELLDME
Sbjct: 432 LTSQKNFDVRGQNPQFIPFGNGRRMCPAISFALQIIYLTLSNFLHGFEIDRPSEELLDME 491

Query: 183 ESIGLTSVRKSPLEVVLIPRLPAQVYG 210
           ESIGLTS++K+PLEVVL PRLP+ +YG
Sbjct: 492 ESIGLTSLKKTPLEVVLTPRLPSHLYG 518

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BLAST of Lag0036887 vs. NCBI nr
Match: XP_038899202.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 342.4 bits (877), Expect = 2.7e-90
Identity = 165/206 (80.10%), Postives = 184/206 (89.32%), Query Frame = 0


Query: 3   SIILGAFDTTTITIIWVVSLLLNNEEALRKVQLELDEQIGRERQVKESDVKNLPYLQAIV 62
           ++I+G FDTT+I + W +SLLLNNEEALRK QLELD+QIGRERQVKESD+KNL YLQAIV
Sbjct: 320 NVIIGGFDTTSIIMTWALSLLLNNEEALRKAQLELDKQIGRERQVKESDIKNLSYLQAIV 379

Query: 63  KETLRLYPAAPLLVPHESTEDCTTVGYHIPAGTRLIINAQKLQRDPLVWADPCEFRPERF 122
           KETLRLYPAAPLLVPHES+EDCTT GYHIP GTRLI+N QKLQ+DP VW DPC+FRPERF
Sbjct: 380 KETLRLYPAAPLLVPHESSEDCTTAGYHIPKGTRLIVNVQKLQKDPCVWEDPCKFRPERF 439

Query: 123 LTTQKSFDVRGQSPQLIPFGNGRRMCPGISFALQIIHLTLASLLHGFKIGRPSEELLDME 182
           LT+ K+ DVRGQ+PQLIPFGNGRR CP ISFALQII+LTLA+LLHGFKI R SEEL DME
Sbjct: 440 LTSHKNIDVRGQNPQLIPFGNGRRQCPAISFALQIIYLTLANLLHGFKIDRLSEELFDME 499

Query: 183 ESIGLTSVRKSPLEVVLIPRLPAQVY 209
           ES GLT+ RKSPLEVVL PRLPA +Y
Sbjct: 500 ESDGLTTARKSPLEVVLTPRLPAPIY 525

[Close]

BLAST of Lag0036887 vs. NCBI nr
Match: XP_022940488.1 (cytochrome P450 CYP82D47-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 341.7 bits (875), Expect = 4.6e-90
Identity = 165/206 (80.10%), Postives = 186/206 (90.29%), Query Frame = 0


Query: 3   SIILGAFDTTTITIIWVVSLLLNNEEALRKVQLELDEQIGRERQVKESDVKNLPYLQAIV 62
           ++ILG FDT T+T+ W +SLLLNNEE+L+K QLELDEQ+GRERQV+ESDVKNLPYLQAIV
Sbjct: 311 NMILGGFDTMTVTMTWALSLLLNNEESLKKAQLELDEQVGRERQVQESDVKNLPYLQAIV 370

Query: 63  KETLRLYPAAPLLVPHESTEDCTTVGYHIPAGTRLIINAQKLQRDPLVWADPCEFRPERF 122
           KETLRLYPAAPLLVPHES EDCT  GY+IPAGTRLI++AQKLQ+DP  W +P EFRP+RF
Sbjct: 371 KETLRLYPAAPLLVPHESMEDCTVAGYYIPAGTRLIVHAQKLQKDPSFWENPYEFRPKRF 430

Query: 123 LTTQKSFDVRGQSPQLIPFGNGRRMCPGISFALQIIHLTLASLLHGFKIGRPSEELLDME 182
           LT+QK+ DVRGQSPQLIPFGNGRRMCPGISFALQ++HLTL +LLHGF IGR SEE LDME
Sbjct: 431 LTSQKNVDVRGQSPQLIPFGNGRRMCPGISFALQVMHLTLVNLLHGFNIGRMSEEPLDME 490

Query: 183 ESIGLTSVRKSPLEVVLIPRLPAQVY 209
           ES GLTSVRK+PLEVVL PRLPA VY
Sbjct: 491 ESFGLTSVRKAPLEVVLSPRLPAHVY 516

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BLAST of Lag0036887 vs. ExPASy Swiss-Prot
Match: O49396 (Cytochrome P450 82C3 OS=Arabidopsis thaliana OX=3702 GN=CYP82C3 PE=2 SV=3)

HSP 1 Score: 240.4 bits (612), Expect = 1.9e-62
Identity = 115/207 (55.56%), Postives = 158/207 (76.33%), Query Frame = 0


Query: 3   SIILGAFDTTTITIIWVVSLLLNNEEALRKVQLELDEQIGRERQVKESDVKNLPYLQAIV 62
           ++ILG  +T+  T+ W +SLLLNN++ L+KVQ E+D  +GR+R V++SD+KNL YLQAI+
Sbjct: 305 ALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVGRDRNVEDSDIKNLVYLQAII 364

Query: 63  KETLRLYPAAPLLVPHESTEDCTTVGYHIPAGTRLIINAQKLQRDPLVWADPCEFRPERF 122
           KETLRLYPAAPLL   E+ EDCT  GY++P GTRLI+N  K+QRDP V+ +P EFRPERF
Sbjct: 365 KETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVWKIQRDPKVYMEPNEFRPERF 424

Query: 123 LTTQ-KSFDVRGQSPQLIPFGNGRRMCPGISFALQIIHLTLASLLHGFKIGRPSEELLDM 182
           +T + K FDVRGQ+ +L+PFG+GRR CPG S A+Q++HL LA  LH F++    +  +DM
Sbjct: 425 ITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGLARFLHSFEVKTVLDRPVDM 484

Query: 183 EESIGLTSVRKSPLEVVLIPRLPAQVY 209
            ES GLT  + +PLEV++ PRL  +++
Sbjct: 485 SESPGLTITKATPLEVLINPRLKRELF 511

[Close]

BLAST of Lag0036887 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 1.9e-62
Identity = 113/207 (54.59%), Postives = 156/207 (75.36%), Query Frame = 0


Query: 3   SIILGAFDTTTITIIWVVSLLLNNEEALRKVQLELDEQIGRERQVKESDVKNLPYLQAIV 62
           ++ILG  DT+  T+ W +SLLLNN+E L+K Q E+D  +GR+R V++SD++NL YLQAI+
Sbjct: 317 ALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSDIENLVYLQAII 376

Query: 63  KETLRLYPAAPLLVPHESTEDCTTVGYHIPAGTRLIINAQKLQRDPLVWADPCEFRPERF 122
           KETLRLYPA PLL P E+ EDCT  GY++P GTRLI+N  K+QRDP V+ +P EFRPERF
Sbjct: 377 KETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVYMEPNEFRPERF 436

Query: 123 LTTQ-KSFDVRGQSPQLIPFGNGRRMCPGISFALQIIHLTLASLLHGFKIGRPSEELLDM 182
           +T + K FDVRGQ+ +L+PFG+GRR CPG S A+Q++HL LA  LH F +    +  +DM
Sbjct: 437 ITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLHSFDVKTVMDMPVDM 496

Query: 183 EESIGLTSVRKSPLEVVLIPRLPAQVY 209
            E+ GLT  + +PLEV++ PR+  +++
Sbjct: 497 SENPGLTIPKATPLEVLISPRIKEELF 523

[Close]

BLAST of Lag0036887 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 6.1e-61
Identity = 113/206 (54.85%), Postives = 151/206 (73.30%), Query Frame = 0


Query: 3   SIILGAFDTTTITIIWVVSLLLNNEEALRKVQLELDEQIGRERQVKESDVKNLPYLQAIV 62
           +IILG  DTT  T+ W +SLLLNN  AL+K Q ELD  +GR+R V ESD+  L Y+ AI+
Sbjct: 315 AIILGGADTTWATLTWALSLLLNNPNALKKAQDELDLHVGRDRNVDESDLVKLTYIDAII 374

Query: 63  KETLRLYPAAPLLVPHESTEDCTTVGYHIPAGTRLIINAQKLQRDPLVWADPCEFRPERF 122
           KETLRLYP  PLL P   TEDCT  GYH+ AGTRLI+NA K+QRDPLVW+ P E++PERF
Sbjct: 375 KETLRLYPPGPLLGPRVVTEDCTIAGYHVRAGTRLIVNAWKIQRDPLVWSQPHEYQPERF 434

Query: 123 LTTQKSFDVRGQSPQLIPFGNGRRMCPGISFALQIIHLTLASLLHGFKIGRPSEELLDME 182
           L  ++  D++GQ  +LIPFG+GRR CP IS ALQ++ LTLA +LHGF++  P++  +DM 
Sbjct: 435 L--ERDVDMKGQHFELIPFGSGRRACPAISLALQVLPLTLAHILHGFELRTPNQNKVDMT 494

Query: 183 ESIGLTSVRKSPLEVVLIPRLPAQVY 209
           E+ G+   + +PLEV++ PR+  + +
Sbjct: 495 ETPGIVHAKATPLEVLVAPRISPKCF 518

[Close]

BLAST of Lag0036887 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 4.4e-59
Identity = 110/205 (53.66%), Postives = 143/205 (69.76%), Query Frame = 0


Query: 4   IILGAFDTTTITIIWVVSLLLNNEEALRKVQLELDEQIGRERQVKESDVKNLPYLQAIVK 63
           +ILG  D+T +T+ W +SLLL N  AL K + E+D QIG++  ++ESD+  L YLQAIVK
Sbjct: 320 LILGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDMQIGKDEYIRESDISKLVYLQAIVK 379

Query: 64  ETLRLYPAAPLLVPHESTEDCTTVGYHIPAGTRLIINAQKLQRDPLVWADPCEFRPERFL 123
           ETLRLYP AP   P E TE+C   GYHI  GTRLI N  K+ RDP VW+DP EF+PERFL
Sbjct: 380 ETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHNLWKIHRDPSVWSDPLEFKPERFL 439

Query: 124 TTQKSFDVRGQSPQLIPFGNGRRMCPGISFALQIIHLTLASLLHGFKIGRPSEELLDMEE 183
           TT K  D+RG + +L+PFG+GRR+C G+S  L ++H TLA+LLH F I  PS E +DM E
Sbjct: 440 TTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVHFTLANLLHSFDILNPSAEPVDMTE 499

Query: 184 SIGLTSVRKSPLEVVLIPRLPAQVY 209
             G T+ + +PLE+++ PR     Y
Sbjct: 500 FFGFTNTKATPLEILVKPRQSPNYY 524

[Close]

BLAST of Lag0036887 vs. ExPASy Swiss-Prot
Match: O49394 (Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=1 SV=2)

HSP 1 Score: 225.3 bits (573), Expect = 6.3e-58
Identity = 107/207 (51.69%), Postives = 152/207 (73.43%), Query Frame = 0


Query: 3   SIILGAFDTTTITIIWVVSLLLNNEEALRKVQLELDEQIGRERQVKESDVKNLPYLQAIV 62
           ++ILG  +T+  T+ W +SLLLNN++ L+K Q E+D  +GR+R V++SD++NL Y+QAI+
Sbjct: 316 ALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVEDSDIENLVYIQAII 375

Query: 63  KETLRLYPAAPLLVPHESTEDCTTVGYHIPAGTRLIINAQKLQRDPLVWADPCEFRPERF 122
           KETLRLYPA PLL   E+ EDCT  GY++  GTR+++N  K+QRDP V+ +P EFRPERF
Sbjct: 376 KETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRVYMEPNEFRPERF 435

Query: 123 LTTQ-KSFDVRGQSPQLIPFGNGRRMCPGISFALQIIHLTLASLLHGFKIGRPSEELLDM 182
           +T + K FDVRGQ+ +L+PFG+GRR CPG S A+Q++HL LA  L  F +    +  +DM
Sbjct: 436 ITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQSFDVKTVMDMPVDM 495

Query: 183 EESIGLTSVRKSPLEVVLIPRLPAQVY 209
            ES GLT  + +PLE+++ PRL   +Y
Sbjct: 496 TESPGLTIPKATPLEILISPRLKEGLY 522

[Close]

BLAST of Lag0036887 vs. ExPASy TrEMBL
Match: A0A6J1CCZ4 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111009608 PE=3 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 6.7e-95
Identity = 169/206 (82.04%), Postives = 189/206 (91.75%), Query Frame = 0


Query: 3   SIILGAFDTTTITIIWVVSLLLNNEEALRKVQLELDEQIGRERQVKESDVKNLPYLQAIV 62
           ++ILG FDTTT+T+ W +SLLLNN+EAL+K QLELDEQ+GRERQV ESDVKNL YL+AIV
Sbjct: 319 NMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRERQVNESDVKNLLYLRAIV 378

Query: 63  KETLRLYPAAPLLVPHESTEDCTTVGYHIPAGTRLIINAQKLQRDPLVWADPCEFRPERF 122
           KETLRLYP APLL PHES EDCT VGY+IP GTRLI+N +KLQRDP VW DPCEFRPERF
Sbjct: 379 KETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKLQRDPFVWEDPCEFRPERF 438

Query: 123 LTTQKSFDVRGQSPQLIPFGNGRRMCPGISFALQIIHLTLASLLHGFKIGRPSEELLDME 182
           LT+Q +FDVRGQSPQ IPFGNGRRMCPGISFALQ+IHLTLA+LLHGF+IGRPSEELLDME
Sbjct: 439 LTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLANLLHGFEIGRPSEELLDME 498

Query: 183 ESIGLTSVRKSPLEVVLIPRLPAQVY 209
           ES+GLTSVRK+PLE+VL PRLPAQVY
Sbjct: 499 ESVGLTSVRKTPLEIVLTPRLPAQVY 524

[Close]

BLAST of Lag0036887 vs. ExPASy TrEMBL
Match: A0A6J1C9U5 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111009700 PE=3 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 6.7e-95
Identity = 173/207 (83.57%), Postives = 189/207 (91.30%), Query Frame = 0


Query: 3   SIILGAFDTTTITIIWVVSLLLNNEEALRKVQLELDEQIGRERQVKESDVKNLPYLQAIV 62
           ++ILG FDTTT+T+ WV+SLLLNN+E L+K QLELDEQ+GR R+VKESDVKNL YLQAIV
Sbjct: 352 NMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKESDVKNLLYLQAIV 411

Query: 63  KETLRLYPAAPLLVPHESTEDCTTVGYHIPAGTRLIINAQKLQRDPLVWADPCEFRPERF 122
           KETLRLYPAAPLLVPHES EDCT  GYHIP GTRLI+N QKLQRDPLVW D CEFRPERF
Sbjct: 412 KETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDSCEFRPERF 471

Query: 123 LTTQKSFDVRGQSPQLIPFGNGRRMCPGISFALQIIHLTLASLLHGFKIGRPSEELLDME 182
           LT+QK+FDVRGQSPQLIPF NGRRMCPGISFALQ+IHLTLA+LLHGFKI RPSEE LDME
Sbjct: 472 LTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGFKIDRPSEEPLDME 531

Query: 183 ESIGLTSVRKSPLEVVLIPRLPAQVYG 210
           ES+GLTSVRK+PLEVVL P LPAQVYG
Sbjct: 532 ESVGLTSVRKAPLEVVLTPCLPAQVYG 558

[Close]

BLAST of Lag0036887 vs. ExPASy TrEMBL
Match: A0A6J1FQP6 (cytochrome P450 CYP82D47-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446069 PE=3 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 2.2e-90
Identity = 165/206 (80.10%), Postives = 186/206 (90.29%), Query Frame = 0


Query: 3   SIILGAFDTTTITIIWVVSLLLNNEEALRKVQLELDEQIGRERQVKESDVKNLPYLQAIV 62
           ++ILG FDT T+T+ W +SLLLNNEE+L+K QLELDEQ+GRERQV+ESDVKNLPYLQAIV
Sbjct: 311 NMILGGFDTMTVTMTWALSLLLNNEESLKKAQLELDEQVGRERQVQESDVKNLPYLQAIV 370

Query: 63  KETLRLYPAAPLLVPHESTEDCTTVGYHIPAGTRLIINAQKLQRDPLVWADPCEFRPERF 122
           KETLRLYPAAPLLVPHES EDCT  GY+IPAGTRLI++AQKLQ+DP  W +P EFRP+RF
Sbjct: 371 KETLRLYPAAPLLVPHESMEDCTVAGYYIPAGTRLIVHAQKLQKDPSFWENPYEFRPKRF 430

Query: 123 LTTQKSFDVRGQSPQLIPFGNGRRMCPGISFALQIIHLTLASLLHGFKIGRPSEELLDME 182
           LT+QK+ DVRGQSPQLIPFGNGRRMCPGISFALQ++HLTL +LLHGF IGR SEE LDME
Sbjct: 431 LTSQKNVDVRGQSPQLIPFGNGRRMCPGISFALQVMHLTLVNLLHGFNIGRMSEEPLDME 490

Query: 183 ESIGLTSVRKSPLEVVLIPRLPAQVY 209
           ES GLTSVRK+PLEVVL PRLPA VY
Sbjct: 491 ESFGLTSVRKAPLEVVLSPRLPAHVY 516

[Close]

BLAST of Lag0036887 vs. ExPASy TrEMBL
Match: A0A6J1FPG1 (cytochrome P450 CYP82D47-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446069 PE=3 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 2.2e-90
Identity = 165/206 (80.10%), Postives = 186/206 (90.29%), Query Frame = 0


Query: 3   SIILGAFDTTTITIIWVVSLLLNNEEALRKVQLELDEQIGRERQVKESDVKNLPYLQAIV 62
           ++ILG FDT T+T+ W +SLLLNNEE+L+K QLELDEQ+GRERQV+ESDVKNLPYLQAIV
Sbjct: 311 NMILGGFDTMTVTMTWALSLLLNNEESLKKAQLELDEQVGRERQVQESDVKNLPYLQAIV 370

Query: 63  KETLRLYPAAPLLVPHESTEDCTTVGYHIPAGTRLIINAQKLQRDPLVWADPCEFRPERF 122
           KETLRLYPAAPLLVPHES EDCT  GY+IPAGTRLI++AQKLQ+DP  W +P EFRP+RF
Sbjct: 371 KETLRLYPAAPLLVPHESMEDCTVAGYYIPAGTRLIVHAQKLQKDPSFWENPYEFRPKRF 430

Query: 123 LTTQKSFDVRGQSPQLIPFGNGRRMCPGISFALQIIHLTLASLLHGFKIGRPSEELLDME 182
           LT+QK+ DVRGQSPQLIPFGNGRRMCPGISFALQ++HLTL +LLHGF IGR SEE LDME
Sbjct: 431 LTSQKNVDVRGQSPQLIPFGNGRRMCPGISFALQVMHLTLVNLLHGFNIGRMSEEPLDME 490

Query: 183 ESIGLTSVRKSPLEVVLIPRLPAQVY 209
           ES GLTSVRK+PLEVVL PRLPA VY
Sbjct: 491 ESFGLTSVRKAPLEVVLSPRLPAHVY 516

[Close]

BLAST of Lag0036887 vs. ExPASy TrEMBL
Match: A0A1S3CMD6 (cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502577 PE=3 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 6.5e-90
Identity = 161/207 (77.78%), Postives = 186/207 (89.86%), Query Frame = 0


Query: 3   SIILGAFDTTTITIIWVVSLLLNNEEALRKVQLELDEQIGRERQVKESDVKNLPYLQAIV 62
           ++ILG FDTTTIT+ W + LLLNN+E L+K Q+EL+EQ+GRERQV+E+D+KNLPYLQAIV
Sbjct: 312 NMILGGFDTTTITMTWALCLLLNNKEELKKAQVELNEQVGRERQVEETDLKNLPYLQAIV 371

Query: 63  KETLRLYPAAPLLVPHESTEDCTTVGYHIPAGTRLIINAQKLQRDPLVWADPCEFRPERF 122
           KETLRLYPAAPLLVPHES EDCT  GYHIP GTRLI+N QKLQ+DP+VW DP EFRPERF
Sbjct: 372 KETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQKLQKDPVVWQDPFEFRPERF 431

Query: 123 LTTQKSFDVRGQSPQLIPFGNGRRMCPGISFALQIIHLTLASLLHGFKIGRPSEELLDME 182
           LT+QK+FDVRGQ PQ IPFGNGRRMCP ISFALQII+LTL++ LHGF+I RPSEELLDME
Sbjct: 432 LTSQKNFDVRGQHPQFIPFGNGRRMCPAISFALQIIYLTLSNFLHGFEIDRPSEELLDME 491

Query: 183 ESIGLTSVRKSPLEVVLIPRLPAQVYG 210
           ES GLTS++K+PLEVVL PRLP+ VYG
Sbjct: 492 ESFGLTSLKKTPLEVVLTPRLPSHVYG 518

[Close]

BLAST of Lag0036887 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 240.4 bits (612), Expect = 1.3e-63
Identity = 115/207 (55.56%), Postives = 158/207 (76.33%), Query Frame = 0


Query: 3   SIILGAFDTTTITIIWVVSLLLNNEEALRKVQLELDEQIGRERQVKESDVKNLPYLQAIV 62
           ++ILG  +T+  T+ W +SLLLNN++ L+KVQ E+D  +GR+R V++SD+KNL YLQAI+
Sbjct: 305 ALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVGRDRNVEDSDIKNLVYLQAII 364

Query: 63  KETLRLYPAAPLLVPHESTEDCTTVGYHIPAGTRLIINAQKLQRDPLVWADPCEFRPERF 122
           KETLRLYPAAPLL   E+ EDCT  GY++P GTRLI+N  K+QRDP V+ +P EFRPERF
Sbjct: 365 KETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVWKIQRDPKVYMEPNEFRPERF 424

Query: 123 LTTQ-KSFDVRGQSPQLIPFGNGRRMCPGISFALQIIHLTLASLLHGFKIGRPSEELLDM 182
           +T + K FDVRGQ+ +L+PFG+GRR CPG S A+Q++HL LA  LH F++    +  +DM
Sbjct: 425 ITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGLARFLHSFEVKTVLDRPVDM 484

Query: 183 EESIGLTSVRKSPLEVVLIPRLPAQVY 209
            ES GLT  + +PLEV++ PRL  +++
Sbjct: 485 SESPGLTITKATPLEVLINPRLKRELF 511

[Close]

BLAST of Lag0036887 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 240.4 bits (612), Expect = 1.3e-63
Identity = 113/207 (54.59%), Postives = 156/207 (75.36%), Query Frame = 0


Query: 3   SIILGAFDTTTITIIWVVSLLLNNEEALRKVQLELDEQIGRERQVKESDVKNLPYLQAIV 62
           ++ILG  DT+  T+ W +SLLLNN+E L+K Q E+D  +GR+R V++SD++NL YLQAI+
Sbjct: 317 ALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSDIENLVYLQAII 376

Query: 63  KETLRLYPAAPLLVPHESTEDCTTVGYHIPAGTRLIINAQKLQRDPLVWADPCEFRPERF 122
           KETLRLYPA PLL P E+ EDCT  GY++P GTRLI+N  K+QRDP V+ +P EFRPERF
Sbjct: 377 KETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVYMEPNEFRPERF 436

Query: 123 LTTQ-KSFDVRGQSPQLIPFGNGRRMCPGISFALQIIHLTLASLLHGFKIGRPSEELLDM 182
           +T + K FDVRGQ+ +L+PFG+GRR CPG S A+Q++HL LA  LH F +    +  +DM
Sbjct: 437 ITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLHSFDVKTVMDMPVDM 496

Query: 183 EESIGLTSVRKSPLEVVLIPRLPAQVY 209
            E+ GLT  + +PLEV++ PR+  +++
Sbjct: 497 SENPGLTIPKATPLEVLISPRIKEELF 523

[Close]

BLAST of Lag0036887 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 225.3 bits (573), Expect = 4.5e-59
Identity = 107/207 (51.69%), Postives = 152/207 (73.43%), Query Frame = 0


Query: 3   SIILGAFDTTTITIIWVVSLLLNNEEALRKVQLELDEQIGRERQVKESDVKNLPYLQAIV 62
           ++ILG  +T+  T+ W +SLLLNN++ L+K Q E+D  +GR+R V++SD++NL Y+QAI+
Sbjct: 316 ALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVEDSDIENLVYIQAII 375

Query: 63  KETLRLYPAAPLLVPHESTEDCTTVGYHIPAGTRLIINAQKLQRDPLVWADPCEFRPERF 122
           KETLRLYPA PLL   E+ EDCT  GY++  GTR+++N  K+QRDP V+ +P EFRPERF
Sbjct: 376 KETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRVYMEPNEFRPERF 435

Query: 123 LTTQ-KSFDVRGQSPQLIPFGNGRRMCPGISFALQIIHLTLASLLHGFKIGRPSEELLDM 182
           +T + K FDVRGQ+ +L+PFG+GRR CPG S A+Q++HL LA  L  F +    +  +DM
Sbjct: 436 ITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQSFDVKTVMDMPVDM 495

Query: 183 EESIGLTSVRKSPLEVVLIPRLPAQVY 209
            ES GLT  + +PLE+++ PRL   +Y
Sbjct: 496 TESPGLTIPKATPLEILISPRLKEGLY 522

[Close]

BLAST of Lag0036887 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 206.1 bits (523), Expect = 2.8e-53
Identity = 101/208 (48.56%), Postives = 138/208 (66.35%), Query Frame = 0


Query: 3   SIILGAFDTTTITIIWVVSLLLNNEEALRKVQLELDEQIGRERQVKESDVKNLPYLQAIV 62
           +++L   +T  + ++W VSLLLNN   LRK Q ELD +IG+ER V+E D+K+L YLQAIV
Sbjct: 311 NLVLAGSETAIVVLVWAVSLLLNNPHVLRKAQEELDSKIGKERVVEELDIKDLVYLQAIV 370

Query: 63  KETLRLYPAAPLLVPHESTE--DCTTVGYHIPAGTRLIINAQKLQRDPLVWADPCEFRPE 122
           KET RLYP  PL+      E  D      H+PAGT+L+++A K+ RDP VW++P +F PE
Sbjct: 371 KETFRLYPPVPLVAYRAVVEDFDIAFCKCHVPAGTQLMVSAWKIHRDPNVWSNPEQFEPE 430

Query: 123 RFLTTQKSFDVRGQSPQLIPFGNGRRMCPGISFALQIIHLTLASLLHGFKIGRPSEELLD 182
           RFLT+ +  DV GQS +  PFG GRR CP I   ++++H  L   LH F + RPS + +D
Sbjct: 431 RFLTSNRELDVGGQSYKFFPFGLGRRSCPAIPLGMRMVHYLLVRFLHSFDLARPSSQDVD 490

Query: 183 MEESIGLTSVRKSPLEVVLIPRLPAQVY 209
           M ES GL + + +PLEV +IPRL   +Y
Sbjct: 491 MTESNGLVNHKATPLEVNIIPRLHKSLY 518

[Close]

BLAST of Lag0036887 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 202.6 bits (514), Expect = 3.1e-52
Identity = 99/206 (48.06%), Postives = 139/206 (67.48%), Query Frame = 0


Query: 3   SIILGAFDTTTITIIWVVSLLLNNEEALRKVQLELDEQIGRERQVKESDVKNLPYLQAIV 62
           ++ L   D+T+IT+ W VSLLLNN  AL   Q E+D  +G+ R ++ESD++NL YLQAIV
Sbjct: 309 ALTLTGSDSTSITLTWAVSLLLNNPAALEAAQEEIDNSVGKGRWIEESDIQNLKYLQAIV 368

Query: 63  KETLRLYPAAPLLVPHESTEDCTTVGYHIPAGTRLIINAQKLQRDPLVWADPCEFRPERF 122
           KET RLYP APL    E+ EDC   GY +  GTRL++N  KL RDP +W DP  F+PERF
Sbjct: 369 KETHRLYPPAPLTGIREAREDCFVGGYRVEKGTRLLVNIWKLHRDPKIWPDPKTFKPERF 428

Query: 123 LTTQKSFDVRGQSPQLIPFGNGRRMCPGISFALQIIHLTLASLLHGFKIGRPSEELLDME 182
           +  +   +    + + IPFG+GRR CPG++  L+++H  LA LL GF++ + S+E LDM 
Sbjct: 429 MEDKSQCE--KSNFEYIPFGSGRRSCPGVNLGLRVVHFVLARLLQGFELHKVSDEPLDMA 488

Query: 183 ESIGLTSVRKSPLEVVLIPRLPAQVY 209
           E  GL   + +P+EVV++PRL  ++Y
Sbjct: 489 EGPGLALPKINPVEVVVMPRLDPKLY 512

The following BLAST results are available for this feature:

BLAST of Lag0036887 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022138438.1	1.4e-94	82.04	cytochrome P450 CYP82D47-like [Momordica charantia]	[more]
XP_022138580.1	1.4e-94	83.57	cytochrome P450 CYP82D47-like [Momordica charantia]	[more]
XP_011652397.1	3.8e-92	79.71	cytochrome P450 CYP82D47 [Cucumis sativus] >KGN60056.2 hypothetical protein Csa_...	[more]
XP_038899202.1	2.7e-90	80.10	cytochrome P450 CYP82D47-like [Benincasa hispida]	[more]
XP_022940488.1	4.6e-90	80.10	cytochrome P450 CYP82D47-like isoform X2 [Cucurbita moschata]	[more]

BLAST of Lag0036887 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49396	1.9e-62	55.56	Cytochrome P450 82C3 OS=Arabidopsis thaliana OX=3702 GN=CYP82C3 PE=2 SV=3	[more]
Q9SZ46	1.9e-62	54.59	Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=...	[more]
A0A0N7F297	6.1e-61	54.85	Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP...	[more]
O49858	4.4e-59	53.66	Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1	[more]
O49394	6.3e-58	51.69	Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=...	[more]

BLAST of Lag0036887 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CCZ4	6.7e-95	82.04	cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111009608 PE=...	[more]
A0A6J1C9U5	6.7e-95	83.57	cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111009700 PE=...	[more]
A0A6J1FQP6	2.2e-90	80.10	cytochrome P450 CYP82D47-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1FPG1	2.2e-90	80.10	cytochrome P450 CYP82D47-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A1S3CMD6	6.5e-90	77.78	cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502577 PE=3 SV=1	[more]

BLAST of Lag0036887 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G31950.1	1.3e-63	55.56	cytochrome P450, family 82, subfamily C, polypeptide 3	[more]
AT4G31940.1	1.3e-63	54.59	cytochrome P450, family 82, subfamily C, polypeptide 4	[more]
AT4G31970.1	4.5e-59	51.69	cytochrome P450, family 82, subfamily C, polypeptide 2	[more]
AT2G25160.1	2.8e-53	48.56	cytochrome P450, family 82, subfamily F, polypeptide 1	[more]
AT3G25180.1	3.1e-52	48.06	cytochrome P450, family 82, subfamily G, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 100..124 score: 39.39 coord: 148..171 score: 31.59 coord: 59..77 score: 38.41 coord: 16..42 score: 33.03 coord: 138..148 score: 53.53
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 60..71 score: 46.11 coord: 148..159 score: 31.73 coord: 7..24 score: 29.48 coord: 139..148 score: 57.45
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 4..185 e-value: 2.3E-56 score: 191.5
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..198 e-value: 1.1E-67 score: 230.6
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 3..202
None	No IPR available	PANTHER	PTHR47947:SF26	CYTOCHROME P450	coord: 3..208
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 3..208
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 141..150

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0036887.1	Lag0036887.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098542	defense response to other organism
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen