Lag0036768 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0036768
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Subtilisin-like protease
Location	chr2: 992739 .. 993296 (+)
RNA-Seq Expression	Lag0036768
Synteny	Lag0036768

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTTTGGATACCGGATCGGTAGCCACACCTTATGATTATGGGGCAGGAGAAATATCAACCACTGGAGCATTACAACCAGGACTAGTCTATGAAACTAGTACAATAGACTACTTAAACTTCCTTTGCTATCGAGGTTATAACCTGTCCACCATTAAGAGCATCTCAAATACTGTTCCTGATGGTTTTGATTGCCCCAAGAAATCAACTGCAGACTACATATCCAACATGAACTACCCAACAATAGCAGTGTCCGCATTGACAGGCAAGGAAAGCAAGAAAATAAGCAGAACAGTTACAAATGTTGGTGGAGACGGTGAAACAGTTTACACAGTTAGTGTGGACGCACCTGGGGAAGTAGATGTCAAAGTGATTCCAGAGAAGTTGGAATTTACAAAGAACAATCAGAAGCAGAGTTACAAAGTGGAGTTCACCTCAAAAGTAGCCATACTAAAGGAAGAAGTCTTTGGATCAATAACTTGGACCAATGGAAAATATCGAGTCCGTAGTTCATTTGTCGTAACTAGTAAGAATAGTGATCTAGGAGATGACCTATAA

mRNA sequence

ATGACTTTGGATACCGGATCGGTAGCCACACCTTATGATTATGGGGCAGGAGAAATATCAACCACTGGAGCATTACAACCAGGACTAGTCTATGAAACTAGTACAATAGACTACTTAAACTTCCTTTGCTATCGAGGTTATAACCTGTCCACCATTAAGAGCATCTCAAATACTGTTCCTGATGGTTTTGATTGCCCCAAGAAATCAACTGCAGACTACATATCCAACATGAACTACCCAACAATAGCAGTGTCCGCATTGACAGGCAAGGAAAGCAAGAAAATAAGCAGAACAGTTACAAATGTTGGTGGAGACGGTGAAACAGTTTACACAGTTAGTGTGGACGCACCTGGGGAAGTAGATGTCAAAGTGATTCCAGAGAAGTTGGAATTTACAAAGAACAATCAGAAGCAGAGTTACAAAGTGGAGTTCACCTCAAAAGTAGCCATACTAAAGGAAGAAGTCTTTGGATCAATAACTTGGACCAATGGAAAATATCGAGTCCGTAGTTCATTTGTCGTAACTAGTAAGAATAGTGATCTAGGAGATGACCTATAA

Coding sequence (CDS)

ATGACTTTGGATACCGGATCGGTAGCCACACCTTATGATTATGGGGCAGGAGAAATATCAACCACTGGAGCATTACAACCAGGACTAGTCTATGAAACTAGTACAATAGACTACTTAAACTTCCTTTGCTATCGAGGTTATAACCTGTCCACCATTAAGAGCATCTCAAATACTGTTCCTGATGGTTTTGATTGCCCCAAGAAATCAACTGCAGACTACATATCCAACATGAACTACCCAACAATAGCAGTGTCCGCATTGACAGGCAAGGAAAGCAAGAAAATAAGCAGAACAGTTACAAATGTTGGTGGAGACGGTGAAACAGTTTACACAGTTAGTGTGGACGCACCTGGGGAAGTAGATGTCAAAGTGATTCCAGAGAAGTTGGAATTTACAAAGAACAATCAGAAGCAGAGTTACAAAGTGGAGTTCACCTCAAAAGTAGCCATACTAAAGGAAGAAGTCTTTGGATCAATAACTTGGACCAATGGAAAATATCGAGTCCGTAGTTCATTTGTCGTAACTAGTAAGAATAGTGATCTAGGAGATGACCTATAA

Protein sequence

MTLDTGSVATPYDYGAGEISTTGALQPGLVYETSTIDYLNFLCYRGYNLSTIKSISNTVPDGFDCPKKSTADYISNMNYPTIAVSALTGKESKKISRTVTNVGGDGETVYTVSVDAPGEVDVKVIPEKLEFTKNNQKQSYKVEFTSKVAILKEEVFGSITWTNGKYRVRSSFVVTSKNSDLGDDL

Homology

[Close]

BLAST of Lag0036768 vs. NCBI nr
Match: XP_022138094.1 (CO(2)-response secreted protease [Momordica charantia])

HSP 1 Score: 314.3 bits (804), Expect = 7.0e-82
Identity = 158/183 (86.34%), Postives = 167/183 (91.26%), Query Frame = 0


Query: 1   MTLDTGSVATPYDYGAGEISTTGALQPGLVYETSTIDYLNFLCYRGYNLSTIKSISNTVP 60
           MTLDTGSVATPYDYGAGEISTTGALQPGLVYET   DYLN+LC RGYNLS IKSIS TVP
Sbjct: 593 MTLDTGSVATPYDYGAGEISTTGALQPGLVYETDITDYLNYLCSRGYNLSIIKSISKTVP 652

Query: 61  DGFDCPKKSTADYISNMNYPTIAVSALTGKESKKISRTVTNVGGDGETVYTVSVDAPGEV 120
           DGFDCPKK TADYISNMNYPTIAVS L GKESKKISRTVTNVGG+GETVYTVSVDA GE+
Sbjct: 653 DGFDCPKKPTADYISNMNYPTIAVSELKGKESKKISRTVTNVGGNGETVYTVSVDAAGEL 712

Query: 121 DVKVIPEKLEFTKNNQKQSYKVEFTSKVAILKEEVFGSITWTNGKYRVRSSFVVTSKNSD 180
           DV+VIPEKLEFTKNNQKQSY+V FTS V  LK++VFGSITWTNGKYRVRS FVVTSK+S+
Sbjct: 713 DVRVIPEKLEFTKNNQKQSYQVVFTSTVGTLKKDVFGSITWTNGKYRVRSPFVVTSKSSE 772

Query: 181 LGD 184
           L D
Sbjct: 773 LED 775

[Close]

BLAST of Lag0036768 vs. NCBI nr
Match: XP_023535470.1 (CO(2)-response secreted protease [Cucurbita pepo subsp. pepo])

HSP 1 Score: 302.4 bits (773), Expect = 2.7e-78
Identity = 153/179 (85.47%), Postives = 162/179 (90.50%), Query Frame = 0


Query: 1   MTLDTGSVATPYDYGAGEISTTGALQPGLVYETSTIDYLNFLCYRGYNLSTIKSISNTVP 60
           MTLDTGSVATPYDYGAGEISTTGAL+PGLVYETST DYL +LC RGYN STIKSISNTVP
Sbjct: 590 MTLDTGSVATPYDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSISNTVP 649

Query: 61  DGFDCPKKSTADYISNMNYPTIAVSALTGKESKKISRTVTNVGGDGETVYTVSVDAPGEV 120
           DGFDCPK S ADYISNMNYPTIAVS L G ESKKI RTVTNVGGDG TVY VSVDAPGEV
Sbjct: 650 DGFDCPKDSNADYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEV 709

Query: 121 DVKVIPEKLEFTKNNQKQSYKVEFTSKVAILKEEVFGSITWTNGKYRVRSSFVVTSKNS 180
           DVKVIPEKLEF+KNNQKQSYKV FTS V+ LK+E FGSITW+NGK+RVRS FVVTS++S
Sbjct: 710 DVKVIPEKLEFSKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESS 768

[Close]

BLAST of Lag0036768 vs. NCBI nr
Match: XP_022937005.1 (CO(2)-response secreted protease [Cucurbita moschata])

HSP 1 Score: 301.6 bits (771), Expect = 4.7e-78
Identity = 153/183 (83.61%), Postives = 163/183 (89.07%), Query Frame = 0


Query: 1   MTLDTGSVATPYDYGAGEISTTGALQPGLVYETSTIDYLNFLCYRGYNLSTIKSISNTVP 60
           MTLDTGSVATPYDYGAGEISTTGAL+PGLVYETST DYL +LC RGYN STIKSI+NTVP
Sbjct: 590 MTLDTGSVATPYDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVP 649

Query: 61  DGFDCPKKSTADYISNMNYPTIAVSALTGKESKKISRTVTNVGGDGETVYTVSVDAPGEV 120
           DGFDCPK S ADYISNMNYPTIAVS L G ESKKI RTVTNVGGDG TVY VSVDAPGEV
Sbjct: 650 DGFDCPKDSNADYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEV 709

Query: 121 DVKVIPEKLEFTKNNQKQSYKVEFTSKVAILKEEVFGSITWTNGKYRVRSSFVVTSKNSD 180
           DVKVIPEKLEF+KNNQKQSYKV FTS V+ LK+E FGSITW+NGK+RVRS FVVTS++S 
Sbjct: 710 DVKVIPEKLEFSKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSK 769

Query: 181 LGD 184
             D
Sbjct: 770 SED 772

[Close]

BLAST of Lag0036768 vs. NCBI nr
Match: KAG6591258.1 (CO(2)-response secreted protease, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 301.6 bits (771), Expect = 4.7e-78
Identity = 153/183 (83.61%), Postives = 163/183 (89.07%), Query Frame = 0


Query: 1   MTLDTGSVATPYDYGAGEISTTGALQPGLVYETSTIDYLNFLCYRGYNLSTIKSISNTVP 60
           MTLDTGSVATPYDYGAGEISTTGAL+PGLVYETST DYL +LC RGYN STIKSI+NTVP
Sbjct: 590 MTLDTGSVATPYDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVP 649

Query: 61  DGFDCPKKSTADYISNMNYPTIAVSALTGKESKKISRTVTNVGGDGETVYTVSVDAPGEV 120
           DGFDCPK S ADYISNMNYPTIAVS L G ESKKI RTVTNVGGDG TVY VSVDAPGEV
Sbjct: 650 DGFDCPKDSNADYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEV 709

Query: 121 DVKVIPEKLEFTKNNQKQSYKVEFTSKVAILKEEVFGSITWTNGKYRVRSSFVVTSKNSD 180
           DVKVIPEKLEF+KNNQKQSYKV FTS V+ LK+E FGSITW+NGK+RVRS FVVTS++S 
Sbjct: 710 DVKVIPEKLEFSKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSK 769

Query: 181 LGD 184
             D
Sbjct: 770 SED 772

[Close]

BLAST of Lag0036768 vs. NCBI nr
Match: KAG7024139.1 (CO(2)-response secreted protease, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 301.6 bits (771), Expect = 4.7e-78
Identity = 153/183 (83.61%), Postives = 163/183 (89.07%), Query Frame = 0


Query: 1   MTLDTGSVATPYDYGAGEISTTGALQPGLVYETSTIDYLNFLCYRGYNLSTIKSISNTVP 60
           MTLDTGSVATPYDYGAGEISTTGAL+PGLVYETST DYL +LC RGYN STIKSI+NTVP
Sbjct: 578 MTLDTGSVATPYDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVP 637

Query: 61  DGFDCPKKSTADYISNMNYPTIAVSALTGKESKKISRTVTNVGGDGETVYTVSVDAPGEV 120
           DGFDCPK S ADYISNMNYPTIAVS L G ESKKI RTVTNVGGDG TVY VSVDAPGEV
Sbjct: 638 DGFDCPKDSNADYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEV 697

Query: 121 DVKVIPEKLEFTKNNQKQSYKVEFTSKVAILKEEVFGSITWTNGKYRVRSSFVVTSKNSD 180
           DVKVIPEKLEF+KNNQKQSYKV FTS V+ LK+E FGSITW+NGK+RVRS FVVTS++S 
Sbjct: 698 DVKVIPEKLEFSKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSK 757

Query: 181 LGD 184
             D
Sbjct: 758 SED 760

[Close]

BLAST of Lag0036768 vs. ExPASy Swiss-Prot
Match: Q9LNU1 (CO(2)-response secreted protease OS=Arabidopsis thaliana OX=3702 GN=CRSP PE=2 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 1.4e-53
Identity = 99/179 (55.31%), Postives = 136/179 (75.98%), Query Frame = 0


Query: 1   MTLDTGSVATPYDYGAGEISTTGALQPGLVYETSTIDYLNFLCYRGYNLSTIKSISNTVP 60
           +T +TG+ ATPYD GAGE+S+T ++QPGLVYET+  DYLNFLCY GYN++TIK++S   P
Sbjct: 589 ITTETGATATPYDSGAGELSSTASMQPGLVYETTETDYLNFLCYYGYNVTTIKAMSKAFP 648

Query: 61  DGFDCPKKSTADYISNMNYPTIAVSALTGKESKKISRTVTNVGGDGETVYTVSVDAPGEV 120
           + F CP  S  D IS +NYP+I +S   G  SK ++RTVTNVG DGE VYTVSV+ P   
Sbjct: 649 ENFTCPADSNLDLISTINYPSIGISGFKGNGSKTVTRTVTNVGEDGEAVYTVSVETPPGF 708

Query: 121 DVKVIPEKLEFTKNNQKQSYKVEFTSKVAILKEEVFGSITWTNGKYRVRSSFVVTSKNS 180
           +++V PEKL+FTK+ +K +Y+V   S  A LK++VFG++TW+N KY+VRS  V++S++S
Sbjct: 709 NIQVTPEKLQFTKDGEKLTYQV-IVSATASLKQDVFGALTWSNAKYKVRSPIVISSESS 766

[Close]

BLAST of Lag0036768 vs. ExPASy Swiss-Prot
Match: F4HSQ2 (Subtilisin-like protease SBT5.1 OS=Arabidopsis thaliana OX=3702 GN=SBT5.1 PE=3 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 1.7e-51
Identity = 101/184 (54.89%), Postives = 133/184 (72.28%), Query Frame = 0


Query: 1   MTLDTGSVATPYDYGAGEISTTGALQPGLVYETSTIDYLNFLCYRGYNLSTIKSISNTVP 60
           +T +TG  ATPYD+GAG+++  G   PGL+YET+ +DYLNFL Y G+    IK ISN +P
Sbjct: 593 ITTETGEKATPYDFGAGQVTIFGPSSPGLIYETNHMDYLNFLGYYGFTSDQIKKISNRIP 652

Query: 61  DGFDCPKKSTADYISNMNYPTIAVSALTGKESKKISRTVTNVG----GDGETVYTVSVDA 120
            GF CP++S    ISN+NYP+I++S   GKES+++SRTVTNV     GD +TVYTVS+DA
Sbjct: 653 QGFACPEQSNRGDISNINYPSISISNFNGKESRRVSRTVTNVASRLIGDEDTVYTVSIDA 712

Query: 121 PGEVDVKVIPEKLEFTKNNQKQSYKVEFTSKVAILKEEVFGSITWTNGKYRVRSSFVVTS 180
           P  + V+VIP +L F K   K SY+V F+S   ILK++ FGSITW+NG Y VRS FVVTS
Sbjct: 713 PEGLLVRVIPRRLHFRKIGDKLSYQVIFSSTTTILKDDAFGSITWSNGMYNVRSPFVVTS 772

[Close]

BLAST of Lag0036768 vs. ExPASy Swiss-Prot
Match: A9JQS7 (Subtilisin-like serine-protease S OS=Lotus japonicus OX=34305 GN=SbtS PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.5e-26
Identity = 71/165 (43.03%), Postives = 100/165 (60.61%), Query Frame = 0


Query: 6   GSVATPYDYGAGEISTTGALQPGLVYETSTIDYLNFLCYRGYNLSTIKSISNTVPDGFDC 65
           G+ ATP+DYG+G ++   +L PGLVY+ S+ D LNFLC  G + + +K+++  +     C
Sbjct: 594 GTQATPFDYGSGHVNPVASLNPGLVYDFSSQDVLNFLCSNGASPAQLKNLTGELT---QC 653

Query: 66  PKKSTADYISNMNYPTIAVSALTGKESKKISRTVTNVGGDGETVYTVSVDAPGEVDVKVI 125
            K  TA Y  N NYP+I VS L G  S  + RTVT  G +  T Y  SV+ P  V V+V 
Sbjct: 654 QKSPTASY--NFNYPSIGVSNLNG--SLSVYRTVTYYGQE-PTEYFASVERPSGVIVRVT 713

Query: 126 PEKLEFTKNNQKQSYKVEFTSKVAILKEEVFGSITWTNGKYRVRS 171
           P KL+F K  +K +++++FT         VFG++TW NGK RVRS
Sbjct: 714 PAKLKFWKAGEKITFRIDFTPFKNSNGNFVFGALTWNNGKQRVRS 750

[Close]

BLAST of Lag0036768 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.1e-24
Identity = 72/171 (42.11%), Postives = 97/171 (56.73%), Query Frame = 0


Query: 5   TGSVATPYDYGAGEISTTGALQPGLVYETSTIDYLNFLCYRGYNLSTIKSISNTVPDGFD 64
           T   ATP+ +GAG +    A+ PGLVY+    DYLNFLC  GYN S I   S    + F 
Sbjct: 608 TNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSG---NNFT 667

Query: 65  CPKKSTADYISNMNYPTIAVSALTGKESKKISRTVTNVGGDGETVYTVSVDAPGEVDVKV 124
           C     +  + N+NYP+I V  LT  +   +SRTV NVG    ++YTV V+ P  V V V
Sbjct: 668 CSSPKIS--LVNLNYPSITVPNLTSSK-VTVSRTVKNVG--RPSMYTVKVNNPQGVYVAV 727

Query: 125 IPEKLEFTKNNQKQSYKVEFT-SKVAILKEEVFGSITWTNGKYRVRSSFVV 175
            P  L FTK  +++++KV    SK  + K  VFG + W++ K+RVRS  VV
Sbjct: 728 KPTSLNFTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVV 770

[Close]

BLAST of Lag0036768 vs. ExPASy Swiss-Prot
Match: Q9STQ2 (Subtilisin-like protease SBT3.18 OS=Arabidopsis thaliana OX=3702 GN=SBT3.18 PE=3 SV=2)

HSP 1 Score: 110.2 bits (274), Expect = 2.6e-23
Identity = 70/181 (38.67%), Postives = 97/181 (53.59%), Query Frame = 0


Query: 1   MTLDTGSVAT--PYDYGAGEISTTGALQPGLVYETSTIDYLNFLCYRGYNLSTIKSISNT 60
           + L  GS+ +  P+D GAG I+   A+ PGLVY T T DY+ F+C  GY    IKS+   
Sbjct: 595 LILSGGSMKSTDPFDIGAGHINPLKAMDPGLVYNTRTDDYVLFMCNIGYTDQEIKSMVLH 654

Query: 61  VPDGFDCPKKSTADYISNMNYPTIAVSALTGKESKKISRTVTNVGGDGETVYTVSVDAPG 120
                 C    +    ++ NYP+I + +L  + ++ I RTV+NVG +  TVY V +  P 
Sbjct: 655 PEPSTTCLPSHSYRTNADFNYPSITIPSL--RLTRTIKRTVSNVGPNKNTVYFVDIIRPV 714

Query: 121 EVDVKVIPEKLEFTKNNQKQSYKVEFTSKVAILKEEVFGSITWTNGKYRVRSSFVVTSKN 180
            V+V + P  L F+K  Q+ SY V F          VFG I WTNG +RVRS  VV   N
Sbjct: 715 GVEVLIWPRILVFSKCQQEHSYYVTFKPTEIFSGRYVFGEIMWTNGLHRVRSPVVVFLSN 773

The following BLAST results are available for this feature:

BLAST of Lag0036768 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022138094.1	7.0e-82	86.34	CO(2)-response secreted protease [Momordica charantia]	[more]
XP_023535470.1	2.7e-78	85.47	CO(2)-response secreted protease [Cucurbita pepo subsp. pepo]	[more]
XP_022937005.1	4.7e-78	83.61	CO(2)-response secreted protease [Cucurbita moschata]	[more]
KAG6591258.1	4.7e-78	83.61	CO(2)-response secreted protease, partial [Cucurbita argyrosperma subsp. sororia...	[more]
KAG7024139.1	4.7e-78	83.61	CO(2)-response secreted protease, partial [Cucurbita argyrosperma subsp. argyros...	[more]

BLAST of Lag0036768 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LNU1	1.4e-53	55.31	CO(2)-response secreted protease OS=Arabidopsis thaliana OX=3702 GN=CRSP PE=2 SV...	[more]
F4HSQ2	1.7e-51	54.89	Subtilisin-like protease SBT5.1 OS=Arabidopsis thaliana OX=3702 GN=SBT5.1 PE=3 S...	[more]
A9JQS7	1.5e-26	43.03	Subtilisin-like serine-protease S OS=Lotus japonicus OX=34305 GN=SbtS PE=2 SV=1	[more]
Q9ZSP5	3.1e-24	42.11	Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=...	[more]
Q9STQ2	2.6e-23	38.67	Subtilisin-like protease SBT3.18 OS=Arabidopsis thaliana OX=3702 GN=SBT3.18 PE=3...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR041469	Subtilisin-like protease, fibronectin type-III domain	PFAM	PF17766	fn3_6	coord: 76..174 e-value: 2.7E-28 score: 98.1
None	No IPR available	GENE3D	2.60.40.2310		coord: 42..177 e-value: 7.9E-36 score: 124.7
None	No IPR available	PANTHER	PTHR10795:SF647	SUBTILISIN-LIKE PROTEASE SBT5.1	coord: 2..176
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 2..176

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0036768.1	Lag0036768.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0009610	response to symbiotic fungus
molecular_function	GO:0004252	serine-type endopeptidase activity