Lag0036767 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0036767
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCO(2)-response secreted protease
Locationchr2: 989593 .. 992682 (+)
RNA-Seq ExpressionLag0036767
SyntenyLag0036767
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCACAGCATTTCTTTTTGTTTCTTCGTTTTGGTTTGTGCCATCTTTTTCCTTCTCTCTGAAAATGGTGAAATAGCTGCAGCTGCTCAAGGTGGAAACAATGGCGTTTACATCGTCTACATGGGATCGGCGTCTGGTTCGAGGACCGACTTCCTTCGCCTTCTTAACTCTGTCAACAGGTGGTAACATATATTAAATATCCCATTTCTTAAAAAATAATTCAATGCTCTTTACCTTAATCAGGAACTTATTATTTCTTCTTCTTCTGTGGTACGTAGGAATCGAAATGCAGTGGTGCACACGTATAAACATGGATTCACAGGATTTGCAGCTCGAATGTCAGAACAGGAAGCCGAGGCCATGAGACATAGACCTGGAGTTGTTTCGGTTTTTCGAGATCCTCTGTTGAAGTTGCACACAACTCATTCGTGGGATTTCTTGATCAGTCAGACAAGTGTGAAGATCGATGCCAATCCTAAATCCGATCCCCCCAGCTCTTCCTCTCAACCATACGATACCATTATCGGCATCTTGGACACCGGTAAATAAATGACATGTGTATATATATATATATATATATATATCAAACATTCTCTACTGCAGCATGTAACATAACAAGTGGTTCTATTCTAACAACTCGCCGTAAAATACAGGAATCTGGCCGGAATCGGAGAGTTTTAACGACAAAGGTATGAAGCCAATACCGTCGAGGTGGAAAGGTACCTGCATGGTAGGCAATGACTTCACCTCCTCCAACTGTAATAAGTAAGTGTGTGCCACAAGTGTCTGAGTGGTTTTCTTTTTTTCAATTTATTTCCTATTATTTAAAGATTTTATAATTTTCTCCTTTAAATTTTGAATAATACCTATCTATTGAATTTTCTCAAAGAAAAAAAAAAAAAAAAACCTATCTATTGAATAGTAGGTATTTTCCAACTAACCAATGTATAAAATTTGACAGGAAAGAACAATCTTCCTATCATTTTACAACTCCTCCCTCTTAGTTAACAACTATTTTTTTTTAAAAAAAAATATGATGCCTTATTCCTTTTTGCACTATTTTAAAAAGAAACTATCACGTTTAATTAATTTATTGTCTTTTGCATTATTCTTCTCTTCTCAAATGTTTAAAATTGGAAAAAATTCAATATTTATAATTTTTCATAGCTCAACTGGCATAAGATTTGTGGTTTGAATCCCCTACGTCCCATATGTTGTAAAAAAAAATCTAGTTAATCAATGATATAATTTATCGTATGTCAATTTCTATATTTTTTTTTTTGAAAAAATGTCAATTTCTATATTAAAGATATAACTAAAAGAATGTTAATGTAAATAATTAATTAACTATAGAGACTAAATTGAAAAAATATGCATGACACTAAGACATATAGCTTTAAATTGTCCTCGGATCATAGTGTGTTTGATATGTTTGTTGTGTCAGAAAAATCATTGGAGCAAGATTTTACGAAAGTTCTGAGAGCGACGGGATACAGTACCACTCACCTCGAGACGAAGCTGGACATGGCACACACGTGGCTTCAACGGCAGCCGGCAGCGCAGTGGCCAATGCTTCCTACTATGGCCTCGCAGCCGGGACGGCCAAGGGCGGCTCCCCCGGCTCAAGGATCGCCATGTATAGAGTCTGTATGGCCGACGGCTGCCGTGGGTCAGCCATCATGGCGGCGTTTGACGATGCCATCGCCGATGGGGTCGATTGCTTGTCCTTATCTCTCGGTACACCCTACTACTTCAAACAGGGACTGAAGGACGACCCTATTGCCATTGGGGCCTTTCATGCAGTTGAGAAGGGGATTGTAGTTGTCTGCTCTGCTGGAAACGACGGCCCCACCTCTGGATCGGTGGTGAATGACGCGCCATGGATTTTAACAGTAGCTGCCTCCACCATTGATCGGGTTTTTGAATCTGATGTTGTGTTGGGCAACAAAAAAGTGATCAAGGTAATTCAAATTCCTTCAACTGCAATTAAACAGCCAAATTGCATCGAGTCATTTCAGATAAATTGACCCAAAAAAATATGTGCATATATGAAGGGAGAAGGTATAAATTTCTCTGCTCTGAAAAAATCCCCTGTATACCCACTGATACAAGGCAAGTCAGCCAAGAAAGGAGATGCCAGTGAAGACAGTGCGAGGTATGTAAAAAGATGGAAACATCATCATGATGCTGACTGACTAAGATTTCCCACAAAGCTTTCTGATGAGCTCTCTATTTAGGATTTGTTCTGAAGACTCCATGGACGAAGCTCTGGTGAAGGGGAAGATTGTTATATGCGAAAGCAATGTCGAAGGAGGCGGTTCCAATTGGCAGGGTCAGGCTGAAACGGTGAAGAGTCTTGGAGGGATCGGTGTAGTTTTGATTGATGACCAATCAAAATTAATTGGAGAGAAGTTTGGAACTTTTCCCATGACAGCTATTGGCTCAAAGGATGGTGCTGAGGTCCTCTCCTATGTCAAGTCAAGCGGGTAAAAGTTAAAATCATTCTCAGTTTAATCAACCTAGATTGGAAACGTGGATGTGAAGAGAGCTTTAATCCCATATTTTGCTTTAGTAGCTTGAACTTTCTGATCTTCAATGGCTAATTGAGTTTGATATTTTCTGAAGGAATCCAGTTGCTACAATCCTACCCTCCGTGACCATAATCAACTATAAGCCAGCGCCTGCTATAGCGTACTTTTCATCTAGAGGGCCTAATCCCAAAGTACTAAACATAATGAAGGTGAAATTTTATCCCTCACTAGAGATTTAAGGAGACGCGTGAATTTGACATCTGAGTTAAATGTGATGCAGCCGGACATATCAGCACCAGGAGTGAACATTCTTGCAGCGTGGCTTGGCAATGACTCGAGCTCTACTCCACAAGGAACAAAGTCGCCACTCTTCAATATGATCTCAGGGACTTCAATGTCCTGCCCGCATGTCTCCGGCGTAGTGGCCTTCGTTAAATCTCAAAACCCCACATGGAGCCCCTCAGCAATCAAGTCGGCCATCATGACAACAGGCAAGAAATTAATCTCAGCAATGAAGAAAAAACTATTCTTTTCTTTTGTTGAATTGAATGTTTACTGA

mRNA sequence

ATGCACAGCATTTCTTTTTGTTTCTTCGTTTTGGTTTGTGCCATCTTTTTCCTTCTCTCTGAAAATGGTGAAATAGCTGCAGCTGCTCAAGGTGGAAACAATGGCGTTTACATCGTCTACATGGGATCGGCGTCTGGTTCGAGGACCGACTTCCTTCGCCTTCTTAACTCTGTCAACAGGAATCGAAATGCAGTGGTGCACACGTATAAACATGGATTCACAGGATTTGCAGCTCGAATGTCAGAACAGGAAGCCGAGGCCATGAGACATAGACCTGGAGTTGTTTCGGTTTTTCGAGATCCTCTGTTGAAGTTGCACACAACTCATTCGTGGGATTTCTTGATCAGTCAGACAAGTGTGAAGATCGATGCCAATCCTAAATCCGATCCCCCCAGCTCTTCCTCTCAACCATACGATACCATTATCGGCATCTTGGACACCGGAATCTGGCCGGAATCGGAGAGTTTTAACGACAAAGGTATGAAGCCAATACCGTCGAGGTGGAAAGGTACCTGCATGGTAGGCAATGACTTCACCTCCTCCAACTGTAATAAAAAAATCATTGGAGCAAGATTTTACGAAAGTTCTGAGAGCGACGGGATACAGTACCACTCACCTCGAGACGAAGCTGGACATGGCACACACGTGGCTTCAACGGCAGCCGGCAGCGCAGTGGCCAATGCTTCCTACTATGGCCTCGCAGCCGGGACGGCCAAGGGCGGCTCCCCCGGCTCAAGGATCGCCATGTATAGAGTCTGTATGGCCGACGGCTGCCGTGGGTCAGCCATCATGGCGGCGTTTGACGATGCCATCGCCGATGGGGTCGATTGCTTGTCCTTATCTCTCGGTACACCCTACTACTTCAAACAGGGACTGAAGGACGACCCTATTGCCATTGGGGCCTTTCATGCAGTTGAGAAGGGGATTGTAGTTGTCTGCTCTGCTGGAAACGACGGCCCCACCTCTGGATCGGTGGTGAATGACGCGCCATGGATTTTAACAGTAGCTGCCTCCACCATTGATCGGGTTTTTGAATCTGATGTTGTGTTGGGCAACAAAAAAGTGATCAAGGGAGAAGGTATAAATTTCTCTGCTCTGAAAAAATCCCCTGTATACCCACTGATACAAGGCAAGTCAGCCAAGAAAGGAGATGCCAGTGAAGACAGTGCGAGGATTTGTTCTGAAGACTCCATGGACGAAGCTCTGGTGAAGGGGAAGATTGTTATATGCGAAAGCAATGTCGAAGGAGGCGGTTCCAATTGGCAGGGTCAGGCTGAAACGGTGAAGAGTCTTGGAGGGATCGGTGTAGTTTTGATTGATGACCAATCAAAATTAATTGGAGAGAAGTTTGGAACTTTTCCCATGACAGCTATTGGCTCAAAGGATGGTGCTGAGGTCCTCTCCTATGTCAAGTCAAGCGGGAATCCAGTTGCTACAATCCTACCCTCCGTGACCATAATCAACTATAAGCCAGCGCCTGCTATAGCGTACTTTTCATCTAGAGGGCCTAATCCCAAAGTACTAAACATAATGAAGCCGGACATATCAGCACCAGGAGTGAACATTCTTGCAGCGTGGCTTGGCAATGACTCGAGCTCTACTCCACAAGGAACAAAGTCGCCACTCTTCAATATGATCTCAGGGACTTCAATGTCCTGCCCGCATGTCTCCGGCGTAGTGGCCTTCGTTAAATCTCAAAACCCCACATGGAGCCCCTCAGCAATCAAGTCGGCCATCATGACAACAGGCAAGAAATTAATCTCAGCAATGAAGAAAAAACTATTCTTTTCTTTTGTTGAATTGAATGTTTACTGA

Coding sequence (CDS)

ATGCACAGCATTTCTTTTTGTTTCTTCGTTTTGGTTTGTGCCATCTTTTTCCTTCTCTCTGAAAATGGTGAAATAGCTGCAGCTGCTCAAGGTGGAAACAATGGCGTTTACATCGTCTACATGGGATCGGCGTCTGGTTCGAGGACCGACTTCCTTCGCCTTCTTAACTCTGTCAACAGGAATCGAAATGCAGTGGTGCACACGTATAAACATGGATTCACAGGATTTGCAGCTCGAATGTCAGAACAGGAAGCCGAGGCCATGAGACATAGACCTGGAGTTGTTTCGGTTTTTCGAGATCCTCTGTTGAAGTTGCACACAACTCATTCGTGGGATTTCTTGATCAGTCAGACAAGTGTGAAGATCGATGCCAATCCTAAATCCGATCCCCCCAGCTCTTCCTCTCAACCATACGATACCATTATCGGCATCTTGGACACCGGAATCTGGCCGGAATCGGAGAGTTTTAACGACAAAGGTATGAAGCCAATACCGTCGAGGTGGAAAGGTACCTGCATGGTAGGCAATGACTTCACCTCCTCCAACTGTAATAAAAAAATCATTGGAGCAAGATTTTACGAAAGTTCTGAGAGCGACGGGATACAGTACCACTCACCTCGAGACGAAGCTGGACATGGCACACACGTGGCTTCAACGGCAGCCGGCAGCGCAGTGGCCAATGCTTCCTACTATGGCCTCGCAGCCGGGACGGCCAAGGGCGGCTCCCCCGGCTCAAGGATCGCCATGTATAGAGTCTGTATGGCCGACGGCTGCCGTGGGTCAGCCATCATGGCGGCGTTTGACGATGCCATCGCCGATGGGGTCGATTGCTTGTCCTTATCTCTCGGTACACCCTACTACTTCAAACAGGGACTGAAGGACGACCCTATTGCCATTGGGGCCTTTCATGCAGTTGAGAAGGGGATTGTAGTTGTCTGCTCTGCTGGAAACGACGGCCCCACCTCTGGATCGGTGGTGAATGACGCGCCATGGATTTTAACAGTAGCTGCCTCCACCATTGATCGGGTTTTTGAATCTGATGTTGTGTTGGGCAACAAAAAAGTGATCAAGGGAGAAGGTATAAATTTCTCTGCTCTGAAAAAATCCCCTGTATACCCACTGATACAAGGCAAGTCAGCCAAGAAAGGAGATGCCAGTGAAGACAGTGCGAGGATTTGTTCTGAAGACTCCATGGACGAAGCTCTGGTGAAGGGGAAGATTGTTATATGCGAAAGCAATGTCGAAGGAGGCGGTTCCAATTGGCAGGGTCAGGCTGAAACGGTGAAGAGTCTTGGAGGGATCGGTGTAGTTTTGATTGATGACCAATCAAAATTAATTGGAGAGAAGTTTGGAACTTTTCCCATGACAGCTATTGGCTCAAAGGATGGTGCTGAGGTCCTCTCCTATGTCAAGTCAAGCGGGAATCCAGTTGCTACAATCCTACCCTCCGTGACCATAATCAACTATAAGCCAGCGCCTGCTATAGCGTACTTTTCATCTAGAGGGCCTAATCCCAAAGTACTAAACATAATGAAGCCGGACATATCAGCACCAGGAGTGAACATTCTTGCAGCGTGGCTTGGCAATGACTCGAGCTCTACTCCACAAGGAACAAAGTCGCCACTCTTCAATATGATCTCAGGGACTTCAATGTCCTGCCCGCATGTCTCCGGCGTAGTGGCCTTCGTTAAATCTCAAAACCCCACATGGAGCCCCTCAGCAATCAAGTCGGCCATCATGACAACAGGCAAGAAATTAATCTCAGCAATGAAGAAAAAACTATTCTTTTCTTTTGTTGAATTGAATGTTTACTGA

Protein sequence

MHSISFCFFVLVCAIFFLLSENGEIAAAAQGGNNGVYIVYMGSASGSRTDFLRLLNSVNRNRNAVVHTYKHGFTGFAARMSEQEAEAMRHRPGVVSVFRDPLLKLHTTHSWDFLISQTSVKIDANPKSDPPSSSSQPYDTIIGILDTGIWPESESFNDKGMKPIPSRWKGTCMVGNDFTSSNCNKKIIGARFYESSESDGIQYHSPRDEAGHGTHVASTAAGSAVANASYYGLAAGTAKGGSPGSRIAMYRVCMADGCRGSAIMAAFDDAIADGVDCLSLSLGTPYYFKQGLKDDPIAIGAFHAVEKGIVVVCSAGNDGPTSGSVVNDAPWILTVAASTIDRVFESDVVLGNKKVIKGEGINFSALKKSPVYPLIQGKSAKKGDASEDSARICSEDSMDEALVKGKIVICESNVEGGGSNWQGQAETVKSLGGIGVVLIDDQSKLIGEKFGTFPMTAIGSKDGAEVLSYVKSSGNPVATILPSVTIINYKPAPAIAYFSSRGPNPKVLNIMKPDISAPGVNILAAWLGNDSSSTPQGTKSPLFNMISGTSMSCPHVSGVVAFVKSQNPTWSPSAIKSAIMTTGKKLISAMKKKLFFSFVELNVY
Homology
BLAST of Lag0036767 vs. NCBI nr
Match: TYK31472.1 (CO(2)-response secreted protease isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 981.1 bits (2535), Expect = 4.3e-282
Identity = 501/586 (85.49%), Postives = 533/586 (90.96%), Query Frame = 0

Query: 1   MHSISFC-FFVLVCAIFFLLSENGEIAAAAQGGNNGVYIVYMGSASGSRTDFLRLLNSVN 60
           M SISFC F  LVC  F L+SEN E+A AA+   NGVYIVYMGSASGSR DFLRLLNSVN
Sbjct: 1   MQSISFCVFLALVCVTFCLVSENVEVADAAEDAKNGVYIVYMGSASGSRIDFLRLLNSVN 60

Query: 61  RNRNAVVHTYKHGFTGFAARMSEQEAEAMRHRPGVVSVFRDPLLKLHTTHSWDFLISQTS 120
           R RNA+VHTYKHGFTGFAAR+SE EA+AMR  PGVVSVF DP+LKLHTTHSWDFL+SQTS
Sbjct: 61  R-RNALVHTYKHGFTGFAARLSEHEAQAMRQSPGVVSVFPDPILKLHTTHSWDFLVSQTS 120

Query: 121 VKIDANPKSDPPSSSSQPYDTIIGILDTGIWPESESFNDKGMKPIPSRWKGTCMVGNDFT 180
           VK+DANPKSD P+SSSQPYDTIIGILDTGIWPESESFNDKGM PIPSRWKGTCM+G+DFT
Sbjct: 121 VKVDANPKSDSPTSSSQPYDTIIGILDTGIWPESESFNDKGMGPIPSRWKGTCMIGDDFT 180

Query: 181 SSNCNKKIIGARFYESSESDGIQYHSPRDEAGHGTHVASTAAGSAVANASYYGLAAGTAK 240
           SSNCN+KIIGARFYESSESDGI+YHSPRD AGHGTHVASTAAGSAVANASYYGLAAGTAK
Sbjct: 181 SSNCNRKIIGARFYESSESDGIRYHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAK 240

Query: 241 GGSPGSRIAMYRVCMADGCRGSAIMAAFDDAIADGVDCLSLSLGTPYYFKQGLKDDPIAI 300
           GGSP SRIAMYRVCMADGCRGS+I+ AFDD+IADGVD LSLSLG+P + +  L  DPIAI
Sbjct: 241 GGSPRSRIAMYRVCMADGCRGSSILKAFDDSIADGVDVLSLSLGSPSFLRPDLTADPIAI 300

Query: 301 GAFHAVEKGIVVVCSAGNDGPTSGSVVNDAPWILTVAASTIDRVFESDVVLGNKKVIKGE 360
           GAFHAVEKGI VVCSAGNDGP+SG+VVNDAPWILTVAASTIDR FESDVVLGNKKVIKGE
Sbjct: 301 GAFHAVEKGITVVCSAGNDGPSSGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGE 360

Query: 361 GINFSALKKSPVYPLIQGKSAKKGDASEDSARICSEDSMDEALVKGKIVICESNVEGGGS 420
           GINFS L+KSPVYPLIQGKSAKK  ASEDSARICSEDSMDEA VKGKIVICES+VEGGGS
Sbjct: 361 GINFSDLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDEAQVKGKIVICESSVEGGGS 420

Query: 421 NWQGQAETVKSLGGIGVVLIDDQSKLIGEKFGTFPMTAIGSKDGAEVLSYVKSSGNPVAT 480
           +WQ QAETVKSLGG+GVVLIDD SKL+ EKF T PMT I  KDG E+LSYV SS  PVAT
Sbjct: 421 DWQSQAETVKSLGGVGVVLIDDDSKLVAEKF-TTPMTVISKKDGPEILSYVNSSRKPVAT 480

Query: 481 ILPSVTIINYKPAPAIAYFSSRGPNPKVLNIMKPDISAPGVNILAAWLGNDSSSTPQGTK 540
           ILP+ TIINYKPAPAI YFSSRGPNP VLNI+KPDISAPGVNILAAWLGNDSSSTPQ TK
Sbjct: 481 ILPTETIINYKPAPAITYFSSRGPNPAVLNIIKPDISAPGVNILAAWLGNDSSSTPQATK 540

Query: 541 SPLFNMISGTSMSCPHVSGVVAFVKSQNPTWSPSAIKSAIMTTGKK 586
           SPLFN+ISGTSMSCPHVSGVVA VKSQNPTWSPSAIKSAIMTTGK+
Sbjct: 541 SPLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIKSAIMTTGKE 584

BLAST of Lag0036767 vs. NCBI nr
Match: XP_004136374.1 (CO(2)-response secreted protease [Cucumis sativus] >KGN60051.1 hypothetical protein Csa_000867 [Cucumis sativus])

HSP 1 Score: 974.5 bits (2518), Expect = 4.0e-280
Identity = 499/584 (85.45%), Postives = 530/584 (90.75%), Query Frame = 0

Query: 1   MHSISFC-FFVLVCAIFFLLSENGEIAAAAQGGNNGVYIVYMGSA-SGSRTDFLRLLNSV 60
           M SISFC FF LVC  FFL+SEN ++A AA+   NGVYIVYMGSA SG RTDFLRLLNSV
Sbjct: 1   MQSISFCVFFALVCVTFFLVSENVKVADAAEDARNGVYIVYMGSASSGFRTDFLRLLNSV 60

Query: 61  NRNRNAVVHTYKHGFTGFAARMSEQEAEAMRHRPGVVSVFRDPLLKLHTTHSWDFLISQT 120
           NR RNAVVHTYKHGFTGFAA +SE EA+AMR  PGVVSVF DPLLKLHTTHSWDFL+SQT
Sbjct: 61  NR-RNAVVHTYKHGFTGFAAHLSEHEAQAMRQSPGVVSVFPDPLLKLHTTHSWDFLVSQT 120

Query: 121 SVKIDANPKSDPPSSSSQPYDTIIGILDTGIWPESESFNDKGMKPIPSRWKGTCMVGNDF 180
           SVKIDANPKSDPP+SSSQPYDTIIGILDTGIWPESESFND GM PIPSRWKGTCM G+DF
Sbjct: 121 SVKIDANPKSDPPASSSQPYDTIIGILDTGIWPESESFNDMGMGPIPSRWKGTCMTGDDF 180

Query: 181 TSSNCNKKIIGARFYESSESDGIQYHSPRDEAGHGTHVASTAAGSAVANASYYGLAAGTA 240
           TSSNCN+KIIGARFYESSESDGI+YHSPRD AGHGTHVASTAAGSAVANASYYGLAAGTA
Sbjct: 181 TSSNCNRKIIGARFYESSESDGIRYHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTA 240

Query: 241 KGGSPGSRIAMYRVCMADGCRGSAIMAAFDDAIADGVDCLSLSLGTPYYFKQGLKDDPIA 300
           KGGSPGSRIAMYRVCMADGCRGS+IM AFDD+IADGVD LSLSLGTP  F+  L  DPIA
Sbjct: 241 KGGSPGSRIAMYRVCMADGCRGSSIMKAFDDSIADGVDVLSLSLGTPSVFRPDLTADPIA 300

Query: 301 IGAFHAVEKGIVVVCSAGNDGPTSGSVVNDAPWILTVAASTIDRVFESDVVLGNKKVIKG 360
           IGAFHAVEKGI VVCSAGNDGP+SG+VVNDAPWILTVAASTIDR FESDVVLGNKKVIKG
Sbjct: 301 IGAFHAVEKGITVVCSAGNDGPSSGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKG 360

Query: 361 EGINFSALKKSPVYPLIQGKSAKKGDASEDSARICSEDSMDEALVKGKIVICESNVEGGG 420
           EGINFS L+KSPVYPLI+GKSAKK   SEDSARICSEDSMDEA VKGKIVICE++VEGGG
Sbjct: 361 EGINFSDLQKSPVYPLIEGKSAKKASDSEDSARICSEDSMDEAQVKGKIVICENSVEGGG 420

Query: 421 SNWQGQAETVKSLGGIGVVLIDDQSKLIGEKFGTFPMTAIGSKDGAEVLSYVKSSGNPVA 480
           S+WQ QAETVK+LGG+G+VLIDD SKL+ EKF T PMT I  KDG E+LSYV SS  PVA
Sbjct: 421 SDWQSQAETVKNLGGVGLVLIDDDSKLVAEKFST-PMTVISKKDGLEILSYVNSSRKPVA 480

Query: 481 TILPSVTIINYKPAPAIAYFSSRGPNPKVLNIMKPDISAPGVNILAAWLGNDSSSTPQGT 540
           T+LP+ TIINYKPAPAI YFSSRGPNP VLNI+KPDISAPGVNILAAWLGNDSSSTPQ T
Sbjct: 481 TVLPTETIINYKPAPAITYFSSRGPNPAVLNIIKPDISAPGVNILAAWLGNDSSSTPQAT 540

Query: 541 KSPLFNMISGTSMSCPHVSGVVAFVKSQNPTWSPSAIKSAIMTT 583
           KSPLFN+ISGTSMSCPHVSGVVA VKSQNPTWSPSAI+SAIMTT
Sbjct: 541 KSPLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIRSAIMTT 582

BLAST of Lag0036767 vs. NCBI nr
Match: XP_038896398.1 (CO(2)-response secreted protease [Benincasa hispida])

HSP 1 Score: 973.8 bits (2516), Expect = 6.9e-280
Identity = 502/586 (85.67%), Postives = 533/586 (90.96%), Query Frame = 0

Query: 1   MHSISFC-FFVLVCAIFFLLSENGEIAAAAQGG--NNGVYIVYMGSASGSRTDFLRLLNS 60
           M SISFC FFV VC IF L+SEN +I  AA  G  NNGVYIVYMGSAS SRTDFLRLLNS
Sbjct: 1   MQSISFCVFFVFVCVIFSLVSENAKIVTAAAEGPKNNGVYIVYMGSASASRTDFLRLLNS 60

Query: 61  VN-RNRNAVVHTYKHGFTGFAARMSEQEAEAMRHRPGVVSVFRDPLLKLHTTHSWDFLIS 120
           V+ RN  AVVHTYKHGFTGFAA +SEQEA+AMR  PGVVSVF DP+LKLHTTHSWDFL+S
Sbjct: 61  VHRRNGKAVVHTYKHGFTGFAAHLSEQEAQAMRQSPGVVSVFPDPILKLHTTHSWDFLVS 120

Query: 121 QTSVKIDANPKSDPPSSSSQPYDTIIGILDTGIWPESESFNDKGMKPIPSRWKGTCMVGN 180
           QTSVKIDANPKSD   SS QPYDTIIGILDTGIWPESESF+DKGM PIPSRWKGTCMVG+
Sbjct: 121 QTSVKIDANPKSD---SSPQPYDTIIGILDTGIWPESESFSDKGMSPIPSRWKGTCMVGD 180

Query: 181 DFTSSNCNKKIIGARFYESSESDGIQYHSPRDEAGHGTHVASTAAGSAVANASYYGLAAG 240
           DFTSSNCN+KIIGARFYESSESDGI++HSPRD AGHGTHVASTAAGSAVANASYYGLAAG
Sbjct: 181 DFTSSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAG 240

Query: 241 TAKGGSPGSRIAMYRVCMADGCRGSAIMAAFDDAIADGVDCLSLSLGTPYYFKQGLKDDP 300
           TAKGGSPGSRIAMYRVC+ADGC GS+IMAAFDDAIADGVD LSLSLG P YF+  L  DP
Sbjct: 241 TAKGGSPGSRIAMYRVCVADGCHGSSIMAAFDDAIADGVDVLSLSLGAPSYFRPDLTADP 300

Query: 301 IAIGAFHAVEKGIVVVCSAGNDGPTSGSVVNDAPWILTVAASTIDRVFESDVVLGNKKVI 360
           IAIGAFHAVEKGI VVCSAGNDGP+SGSVVNDAPWI+TVAASTIDR FESDVVLGNKKVI
Sbjct: 301 IAIGAFHAVEKGITVVCSAGNDGPSSGSVVNDAPWIITVAASTIDRDFESDVVLGNKKVI 360

Query: 361 KGEGINFSALKKSPVYPLIQGKSAKKGDASEDSARICSEDSMDEALVKGKIVICESNVEG 420
           KGEGINFS L+KSPVYPLIQGKSAKK  ASEDSARICSEDSMDE  VKGKIVICE++VEG
Sbjct: 361 KGEGINFSNLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDETHVKGKIVICENSVEG 420

Query: 421 GGSNWQGQAETVKSLGGIGVVLIDDQSKLIGEKFGTFPMTAIGSKDGAEVLSYVKSSGNP 480
           GGS+WQ QAETVK+LGG+GVVLIDD SKL+ EKF + PMT I  KDGAE+LSYV SSGNP
Sbjct: 421 GGSDWQDQAETVKNLGGVGVVLIDDNSKLVAEKFPS-PMTVISKKDGAELLSYVTSSGNP 480

Query: 481 VATILPSVTIINYKPAPAIAYFSSRGPNPKVLNIMKPDISAPGVNILAAWLGNDSSSTPQ 540
           VATILP+VTIINYKPAPAI YFSSRGPNP VLN++KPDISAPGVNILAAWLGNDSSSTPQ
Sbjct: 481 VATILPTVTIINYKPAPAITYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDSSSTPQ 540

Query: 541 GTKSPLFNMISGTSMSCPHVSGVVAFVKSQNPTWSPSAIKSAIMTT 583
            TKSPLFN+ISGTSMSCPHVSGVVAFVKSQNPTWSPSAI+SAIMTT
Sbjct: 541 ATKSPLFNVISGTSMSCPHVSGVVAFVKSQNPTWSPSAIRSAIMTT 582

BLAST of Lag0036767 vs. NCBI nr
Match: XP_022975820.1 (CO(2)-response secreted protease [Cucurbita maxima])

HSP 1 Score: 961.8 bits (2485), Expect = 2.7e-276
Identity = 494/583 (84.73%), Postives = 529/583 (90.74%), Query Frame = 0

Query: 1   MHSISFCFFVLVCAIFFLLSENGEIAAAAQGGNNGVYIVYMGSASGSRTDFLRLLNSVN- 60
           M +IS C F+LVC IF LLSEN +IAAAA  GNNGVYIVYMGSA  SRTDFLRLL SVN 
Sbjct: 1   MQTISSCAFLLVCVIFLLLSENAKIAAAA-AGNNGVYIVYMGSA--SRTDFLRLLTSVNR 60

Query: 61  RNRNAVVHTYKHGFTGFAARMSEQEAEAMRHRPGVVSVFRDPLLKLHTTHSWDFLISQTS 120
           RN NAVV TYKHGFTGFAAR+SEQEA AMR RPGVVSVF DP+LKLHTTHSWDFL+SQTS
Sbjct: 61  RNGNAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTS 120

Query: 121 VKIDANPKSDPPSSSSQPYDTIIGILDTGIWPESESFNDKGMKPIPSRWKGTCMVGNDFT 180
           VKID+ PKS+PP+SSSQP DTIIGILDTGIWPESESF D+GM PIPSRWKGTCM G+DFT
Sbjct: 121 VKIDSAPKSEPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFT 180

Query: 181 SSNCNKKIIGARFYESSESDGIQYHSPRDEAGHGTHVASTAAGSAVANASYYGLAAGTAK 240
           SSNCN+KIIGARFYESSESDGI++HSPRD AGHGTHVASTAAGSAVANASYYGLAAGTAK
Sbjct: 181 SSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAK 240

Query: 241 GGSPGSRIAMYRVCMADGCRGSAIMAAFDDAIADGVDCLSLSLGTPYYFKQGLKDDPIAI 300
           GGSPGSRIAMYRVCMADGC GS+I+AAFDDAI DGVD LSLSLGTPY++K  L  DPIAI
Sbjct: 241 GGSPGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGTPYFYKPELATDPIAI 300

Query: 301 GAFHAVEKGIVVVCSAGNDGPTSGSVVNDAPWILTVAASTIDRVFESDVVLGNKKVIKGE 360
           GAFHAVEKGI VVCSAGNDGPT GSVVNDAPWILTVAASTIDR FESDVVLGNKKVIKGE
Sbjct: 301 GAFHAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGE 360

Query: 361 GINFSALKKSPVYPLIQGKSAKKGDASEDSARICSEDSMDEALVKGKIVICESNVEGGGS 420
           GINFSAL+ SPVYPLI GKSA K   SEDSARICSE SMDEALVKGKIVICES+ EGGGS
Sbjct: 361 GINFSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGS 420

Query: 421 NWQGQAETVKSLGGIGVVLIDDQSKLIGEKFGTFPMTAIGSKDGAEVLSYVKSSGNPVAT 480
            WQGQAETV+SLGG+G+VLIDDQ+KL+ EKF T P+TAI SKDG EVL+YV SS NPVAT
Sbjct: 421 YWQGQAETVESLGGVGIVLIDDQAKLVAEKF-TSPITAISSKDGDEVLAYVHSSRNPVAT 480

Query: 481 ILPSVTIINYKPAPAIAYFSSRGPNPKVLNIMKPDISAPGVNILAAWLGNDSSSTPQGTK 540
           ILP++T+INYKPAPAIAYFSSRGPNP VLN++KPDISAPGVNILAAWLGND++STPQ   
Sbjct: 481 ILPTLTVINYKPAPAIAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPEN 540

Query: 541 SPLFNMISGTSMSCPHVSGVVAFVKSQNPTWSPSAIKSAIMTT 583
           SPLFN+ISGTSMSCPHVSGVVA VKSQNPTWSPSAIKSAIMTT
Sbjct: 541 SPLFNVISGTSMSCPHVSGVVAMVKSQNPTWSPSAIKSAIMTT 579

BLAST of Lag0036767 vs. NCBI nr
Match: XP_022937005.1 (CO(2)-response secreted protease [Cucurbita moschata])

HSP 1 Score: 960.7 bits (2482), Expect = 6.0e-276
Identity = 493/583 (84.56%), Postives = 528/583 (90.57%), Query Frame = 0

Query: 1   MHSISFCFFVLVCAIFFLLSENGEIAAAAQGGNNGVYIVYMGSASGSRTDFLRLLNSVN- 60
           M +IS C F+LVC IF LLSEN +IAAAA  GNNGVYIVYMGSA  SRTDFLRLL SVN 
Sbjct: 1   MQTISSCVFLLVCVIFLLLSENAKIAAAA-AGNNGVYIVYMGSA--SRTDFLRLLTSVNR 60

Query: 61  RNRNAVVHTYKHGFTGFAARMSEQEAEAMRHRPGVVSVFRDPLLKLHTTHSWDFLISQTS 120
           RN NAVV TYKHGFTGFAAR+SEQEA AMR RPGVVSVF DP+LKLHTTHSWDFL+SQTS
Sbjct: 61  RNGNAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTS 120

Query: 121 VKIDANPKSDPPSSSSQPYDTIIGILDTGIWPESESFNDKGMKPIPSRWKGTCMVGNDFT 180
           VKID+ PK+DPP+SSSQP+DTIIGILDTGIWPESESF D+GM PIPSRWKGTCM G+DFT
Sbjct: 121 VKIDSAPKADPPTSSSQPFDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFT 180

Query: 181 SSNCNKKIIGARFYESSESDGIQYHSPRDEAGHGTHVASTAAGSAVANASYYGLAAGTAK 240
           SSNCN+KIIGARFYESSESDGI++HSPRD AGHGTHVASTAAGSAVANASYYGLAAGTAK
Sbjct: 181 SSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAK 240

Query: 241 GGSPGSRIAMYRVCMADGCRGSAIMAAFDDAIADGVDCLSLSLGTPYYFKQGLKDDPIAI 300
           GGSPGSRIAMYRVCMADGC GS+I+AAFDDAI DGVD LSLSLG P Y+K  L  DPIAI
Sbjct: 241 GGSPGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPNYYKPELTTDPIAI 300

Query: 301 GAFHAVEKGIVVVCSAGNDGPTSGSVVNDAPWILTVAASTIDRVFESDVVLGNKKVIKGE 360
           GAFHAVEKGI VVCSAGNDGPT GSVVNDAPWILTVAASTIDR FESDVVLGNKKVIKGE
Sbjct: 301 GAFHAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGE 360

Query: 361 GINFSALKKSPVYPLIQGKSAKKGDASEDSARICSEDSMDEALVKGKIVICESNVEGGGS 420
           GINFSAL+ SPVYPLI GKSA K   SEDSARICSE SMDEALVKGKIVICES+ EGGGS
Sbjct: 361 GINFSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGS 420

Query: 421 NWQGQAETVKSLGGIGVVLIDDQSKLIGEKFGTFPMTAIGSKDGAEVLSYVKSSGNPVAT 480
            WQGQAETV+SLGG+G+VLIDDQ+KL+ EKF T P+TAI SKDG EVL+YV SS NPVAT
Sbjct: 421 YWQGQAETVESLGGVGIVLIDDQTKLVAEKF-TSPITAISSKDGDEVLAYVHSSRNPVAT 480

Query: 481 ILPSVTIINYKPAPAIAYFSSRGPNPKVLNIMKPDISAPGVNILAAWLGNDSSSTPQGTK 540
           ILP++T+INYKPAPAIAYFSSRGPNP VLN++KPDISAPGVNILAAWLGND++STPQ   
Sbjct: 481 ILPTLTVINYKPAPAIAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPEN 540

Query: 541 SPLFNMISGTSMSCPHVSGVVAFVKSQNPTWSPSAIKSAIMTT 583
           SPLFN+ISGTSMSCPHVSGVVA VKSQNPTWSPSAIKSAIMTT
Sbjct: 541 SPLFNVISGTSMSCPHVSGVVAMVKSQNPTWSPSAIKSAIMTT 579

BLAST of Lag0036767 vs. ExPASy Swiss-Prot
Match: Q9LNU1 (CO(2)-response secreted protease OS=Arabidopsis thaliana OX=3702 GN=CRSP PE=2 SV=1)

HSP 1 Score: 685.3 bits (1767), Expect = 6.4e-196
Identity = 356/579 (61.49%), Postives = 440/579 (75.99%), Query Frame = 0

Query: 8   FFVLVCAIFFLLSENGEIAAAAQGGNNGVYIVYMGSASGSRTDFLR--LLNSV-NRNRNA 67
           F  L+C +F   +E      A     +GVYIVYMGSAS +        L+N++  R  N 
Sbjct: 13  FLYLLCILFMTETE------AGSRNGDGVYIVYMGSASSAANANRAQILINTMFKRRAND 72

Query: 68  VVHTYKHGFTGFAARMSEQEAEAMRHRPGVVSVFRDPLLKLHTTHSWDFLISQTSVKIDA 127
           ++HTYKHGF+GFAAR++ +EA+ +  +PGVVSVF DP  +LHTTHSWDFL  QTSVK+D+
Sbjct: 73  LLHTYKHGFSGFAARLTAEEAKVIAKKPGVVSVFPDPHFQLHTTHSWDFLKYQTSVKVDS 132

Query: 128 NPKSDPPSSSSQPYDTIIGILDTGIWPESESFNDKGMKPIPSRWKGTCMVGNDFTSSNCN 187
            P   P S+S   YD+I+GILDTGIWPESESFNDK M PIPSRWKGTCM   DF SSNCN
Sbjct: 133 GP---PSSASDGSYDSIVGILDTGIWPESESFNDKDMGPIPSRWKGTCMEAKDFKSSNCN 192

Query: 188 KKIIGARFYESSESDGIQYHSPRDEAGHGTHVASTAAGSAVANASYYGLAAGTAKGGSPG 247
           +KIIGAR+Y++ + D  +Y++ RD  GHG+HV+ST AGSAV NASYYG+A+GTAKGGS  
Sbjct: 193 RKIIGARYYKNPDDDS-EYYTTRDVIGHGSHVSSTIAGSAVENASYYGVASGTAKGGSQN 252

Query: 248 SRIAMYRVCMADGCRGSAIMAAFDDAIADGVDCLSLSLGTPYYFKQGLKDDPIAIGAFHA 307
           +RIAMY+VC   GC GS+I+AAFDDAIADGVD LSLSLG P Y +  L  DPIAIGAFHA
Sbjct: 253 ARIAMYKVCNPGGCTGSSILAAFDDAIADGVDVLSLSLGAPAYARIDLNTDPIAIGAFHA 312

Query: 308 VEKGIVVVCSAGNDGPTSGSVVNDAPWILTVAASTIDRVFESDVVLGNKKVIKGEGINFS 367
           VE+GI+V+CSAGNDGP  G+V N APWI+TVAA+TIDR FESDVVLG  KVIKGEGI+FS
Sbjct: 313 VEQGILVICSAGNDGPDGGTVTNTAPWIMTVAANTIDRDFESDVVLGGNKVIKGEGIHFS 372

Query: 368 ALKKSPVYPLIQGKSAKKGDASEDSARICSEDSMDEALVKGKIVICESNVEGGGSNWQGQ 427
            + KSPVYPLI GKSAK  DASE SAR C  DS+D+  VKGKIV+CE NV  GGS +   
Sbjct: 373 NVSKSPVYPLIHGKSAKSADASEGSARACDSDSLDQEKVKGKIVLCE-NV--GGSYYASS 432

Query: 428 A-ETVKSLGGIGVVLIDDQSKLIGEKFGTFPMTAIGSKDGAEVLSYVKSSGNPVATILPS 487
           A + VKS GG G V +DD+++ +   +G+FP T I SK+ AE+ SY+ S+ +PVATILP+
Sbjct: 433 ARDEVKSKGGTGCVFVDDRTRAVASAYGSFPTTVIDSKEAAEIFSYLNSTKDPVATILPT 492

Query: 488 VTIINYKPAPAIAYFSSRGPNPKVLNIMKPDISAPGVNILAAWLGNDSSSTPQGTKSPLF 547
            T+  + PAPA+AYFSSRGP+    +I+KPDI+APGV+ILAAW GNDSS + +G  +  +
Sbjct: 493 ATVEKFTPAPAVAYFSSRGPSSLTRSILKPDITAPGVSILAAWTGNDSSISLEGKPASQY 552

Query: 548 NMISGTSMSCPHVSGVVAFVKSQNPTWSPSAIKSAIMTT 583
           N+ISGTSM+ PHVS V + +KSQ+PTW PSAI+SAIMTT
Sbjct: 553 NVISGTSMAAPHVSAVASLIKSQHPTWGPSAIRSAIMTT 578

BLAST of Lag0036767 vs. ExPASy Swiss-Prot
Match: F4HSQ2 (Subtilisin-like protease SBT5.1 OS=Arabidopsis thaliana OX=3702 GN=SBT5.1 PE=3 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 1.3e-159
Identity = 305/594 (51.35%), Postives = 417/594 (70.20%), Query Frame = 0

Query: 3   SISFCFFVLVCAIFFLLSENGEIAAAAQGGNNGVYIVYMGSAS---GSRTDFLRLLNS-V 62
           +I+  FF     +FF LS   +    ++   +G YI+YMG+AS    +  D + LL+S +
Sbjct: 6   TITIMFF-----MFFFLSVIQK--CKSETSKSGDYIIYMGAASSDGSTDNDHVELLSSLL 65

Query: 63  NRNRNAVVHTYKHGFTGFAARMSEQEAEAMRHRPGVVSVFRDPLLKLHTTHSWDFLISQT 122
            R+    +H YKHGF+GFAA +SE EA  +  +PGV+SVF D +L+LHTT SWDFL+ Q 
Sbjct: 66  QRSGKTPMHRYKHGFSGFAAHLSEDEAHLIAKQPGVLSVFPDQMLQLHTTRSWDFLV-QE 125

Query: 123 SVKIDA--NPKSDPPSSSSQPYDTIIGILDTGIWPESESFNDKGMKPIPSRWKGTCMVGN 182
           S + D      +    S     DTIIG LD+GIWPE++SFND+ M P+P +WKGTCM G 
Sbjct: 126 SYQRDTYFTEMNYEQESEMHEGDTIIGFLDSGIWPEAQSFNDRHMGPVPEKWKGTCMRGK 185

Query: 183 DF--TSSNCNKKIIGARFYESSESDGIQYHSPRDEAGHGTHVASTAAGSAVANASYYGLA 242
                S  CN+K+IGAR+Y SS      Y +PRD  GHGTHVAS AAG  +ANASYYGLA
Sbjct: 186 KTQPDSFRCNRKLIGARYYNSSFFLDPDYETPRDFLGHGTHVASIAAGQIIANASYYGLA 245

Query: 243 AGTAKGGSPGSRIAMYRVCMADGCRGSAIMAAFDDAIADGVDCLSLSLGTPYYFKQGLKD 302
           +G  +GGSP SRIAMYR C   GCRGS+I+AAFDDAIADGVD +S+S+G    +   L +
Sbjct: 246 SGIMRGGSPSSRIAMYRACSLLGCRGSSILAAFDDAIADGVDVISISMG---LWPDNLLE 305

Query: 303 DPIAIGAFHAVEKGIVVVCSAGNDGPTSGSVVNDAPWILTVAASTIDRVFESDVVLG--N 362
           DP++IG+FHAVE+GI VVCS GN GP+S SV N APW++TVAASTIDR FES+++LG   
Sbjct: 306 DPLSIGSFHAVERGITVVCSVGNSGPSSQSVFNAAPWMITVAASTIDRGFESNILLGGDE 365

Query: 363 KKVIKGEGINFSALKKSPVYPLIQGKSAKKGDASEDSARICSEDSMDEALVKGKIVICES 422
            ++I+G GIN + + K+  YPLI  +SAKK DA+E++AR C+ D++D+ +VKGKIV+C+S
Sbjct: 366 NRLIEGFGINIANIDKTQAYPLIHARSAKKIDANEEAARNCAPDTLDQTIVKGKIVVCDS 425

Query: 423 NVEGGGSNWQGQAETVKSLGGIGVVLIDDQSKLIGEKFGTFPMTAIGSKDGAEVLSYVKS 482
           +++     W  +++ VK LGGIG+VL+DD+S  +     +F +T I  +DG +++SY+ S
Sbjct: 426 DLDNQVIQW--KSDEVKRLGGIGMVLVDDESMDLSFIDPSFLVTIIKPEDGIQIMSYINS 485

Query: 483 SGNPVATILPSVTIINYKPAPAIAYFSSRGPNPKVLNIMKPDISAPGVNILAAWLGNDSS 542
           +  P+ATI+P+ +   +  AP+I  FSSRGP     +I+KPDI+APGVNILA+WL  D +
Sbjct: 486 TREPIATIMPTRSRTGHMLAPSIPSFSSRGPYLLTRSILKPDIAAPGVNILASWLVGDRN 545

Query: 543 STPQGTKSPLFNMISGTSMSCPHVSGVVAFVKSQNPTWSPSAIKSAIMTTGKKL 587
           + P+G   PLFN+ SGTSMSCPHVSG+ A +KS+ P+WSP+AI+SAIMTT  ++
Sbjct: 546 AAPEGKPPPLFNIESGTSMSCPHVSGIAARLKSRYPSWSPAAIRSAIMTTAVQM 586

BLAST of Lag0036767 vs. ExPASy Swiss-Prot
Match: A9JQS7 (Subtilisin-like serine-protease S OS=Lotus japonicus OX=34305 GN=SbtS PE=2 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 2.8e-127
Identity = 260/569 (45.69%), Postives = 354/569 (62.21%), Query Frame = 0

Query: 37  YIVYMGSASGSRTDFLRLLN---------SVNRNRNAVVHTYKHGFTGFAARMSEQEAEA 96
           YIVYMG  S   ++ +   N         S+N  + A +H Y   F GF+A ++ ++A+ 
Sbjct: 28  YIVYMGDRSHPNSESVVRANHEILASVTGSLNDAKAAAIHHYSRSFQGFSAMITPEQAKK 87

Query: 97  MRHRPGVVSVFRDPLLKLHTTHSWDFLISQTSVKIDANPKSDPPSSSSQPYDTIIGILDT 156
           +     VVSVF   + KLHTTHSWDFL   T  K       + PS+     + I+G++D+
Sbjct: 88  LADHNSVVSVFESKMNKLHTTHSWDFLGLDTVYK-------NNPSALDSASNVIVGVIDS 147

Query: 157 GIWPESESFNDKGMKPIPSRWKGTCMVGNDFTSSNCNKKIIGARFYESSES--------- 216
           G+WPESESFND G+ P+P ++KG C+ G++FT +NCNKKIIGARFY              
Sbjct: 148 GVWPESESFNDYGLGPVPEKFKGECVTGDNFTLANCNKKIIGARFYSKGLEAEIGPLENI 207

Query: 217 -DGIQYHSPRDEAGHGTHVASTAAGSAVANASYYGLAAGTAKGGSPGSRIAMYRVCMADG 276
            D I + SPRD  GHGTH AST AGS V+N S +G+A GTA+GG+P +R+++Y+ C    
Sbjct: 208 VDSIFFRSPRDSDGHGTHTASTIAGSIVSNVSLFGMAKGTARGGAPSARLSIYKACWFGF 267

Query: 277 CRGSAIMAAFDDAIADGVDCLSLSLG----TPYYFKQGLKDDPIAIGAFHAVEKGIVVVC 336
           C  + + AA DDAI DGVD LSLSLG     P YF     ++ I++GAFHA +KGI+V  
Sbjct: 268 CSDADVFAAMDDAIHDGVDILSLSLGPDPPQPLYF-----ENAISVGAFHAFQKGILVSA 327

Query: 337 SAGNDGPTSGSVVNDAPWILTVAASTIDRVFESDVVLGNKKVIKGEGINFSALKKSPVYP 396
           SAGN      +  N APWI TVAAST+DR F SD+ LGN KV+KG  +N   +K    Y 
Sbjct: 328 SAGN-SVFPRTACNVAPWIFTVAASTVDREFRSDIYLGNSKVLKGLSLN--PIKMEGSYG 387

Query: 397 LIQGKSAKKGDASEDSARICSEDSMDEALVKGKIVICESNVEGGGSNWQGQAETVKSLGG 456
           LI G +A     +  +A  C E ++D  L+KGKIVIC   VE    N + +A  +K  GG
Sbjct: 388 LIYGSAAAAAGDAALNASFCKEHTLDPTLIKGKIVIC--TVEKFTDNRREKAIIIKQGGG 447

Query: 457 IGVVLIDDQSKLIGEKFGTFPMTAIGSKDGAEVLSYVKSSGNPVATILPSVTIINYKPAP 516
           +G++LID  ++ +G +F   P T IG     E+ +Y+K+  NP ATI P++T++  KPAP
Sbjct: 448 VGMILIDHNARDVGFQF-VIPSTMIGQDAVEELQAYMKTEKNPTATIFPTLTLVGTKPAP 507

Query: 517 AIAYFSSRGPNPKVLNIMKPDISAPGVNILAAWLGNDSSSTPQGTKSPLFNMISGTSMSC 576
             A FSS GPN    +I+KPDI+ PGVNILAAW    + +T +  KS  +N+ISGTSMSC
Sbjct: 508 ESAAFSSVGPNIITPDIIKPDITGPGVNILAAWSPVATEATVE-QKSVNYNIISGTSMSC 567

Query: 577 PHVSGVVAFVKSQNPTWSPSAIKSAIMTT 583
           PH+S + A +KS +P+WSP+AI SAIMT+
Sbjct: 568 PHISAISAIIKSHHPSWSPAAIMSAIMTS 577

BLAST of Lag0036767 vs. ExPASy Swiss-Prot
Match: Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 3.4e-120
Identity = 257/557 (46.14%), Postives = 347/557 (62.30%), Query Frame = 0

Query: 36  VYIVYMGSASGSRTDF---------LRLLNSVNRNRNAVVHTYKHGFTGFAARMSEQEAE 95
           VYIVYMGS S SR D+         L+ +   +     +V +YK  F GFAAR++E E  
Sbjct: 32  VYIVYMGSLS-SRADYIPTSDHMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERT 91

Query: 96  AMRHRPGVVSVFRDPLLKLHTTHSWDFLISQTSVKIDANPKSDPPSSSSQPYDTIIGILD 155
            +    GVVSVF + +L+LHTT SWDF+     VK   N K +    S    DTIIG++D
Sbjct: 92  LIAEIEGVVSVFPNKILQLHTTTSWDFM----GVKEGKNTKRNLAIES----DTIIGVID 151

Query: 156 TGIWPESESFNDKGMKPIPSRWKGTCMVGNDFTSSNCNKKIIGARFYESSESDGIQYHSP 215
           TGIWPES+SF+DKG  P P +WKG C  G +FT   CN K+IGAR Y S           
Sbjct: 152 TGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFT---CNNKLIGARDYTS--------EGT 211

Query: 216 RDEAGHGTHVASTAAGSAVANASYYGLAAGTAKGGSPGSRIAMYRVCMADGCRGSAIMAA 275
           RD +GHGTH ASTAAG+AV + S++G+  GT +GG P SRIA Y+VC   GC   A++++
Sbjct: 212 RDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALLSS 271

Query: 276 FDDAIADGVDCLSLSLGTPYYFKQGLKDDPIAIGAFHAVEKGIVVVCSAGNDGPTSGSVV 335
           FDDAIADGVD +++S+G  + F    +DDPIAIGAFHA+ KGI+ V SAGN GP   +V 
Sbjct: 272 FDDAIADGVDLITISIG--FQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVS 331

Query: 336 NDAPWILTVAASTIDRVFESDVVLGNKKVIKGEGINFSALKKSPVYPLIQGKSAKKGDAS 395
           + APWI TVAAST +R F + VVLGN K + G  +N   + K   YPL+ GKSA      
Sbjct: 332 HVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDM-KGKKYPLVYGKSAASSACD 391

Query: 396 EDSARICSEDSMDEALVKGKIVICESNVEGGGSNWQGQAETVKSLGGIGVVLIDDQSKLI 455
             +A +C+   ++++ VKGKI++C     GG S +    +  KS+G I ++   D+S   
Sbjct: 392 AKTAALCAPACLNKSRVKGKILVC-----GGPSGY----KIAKSVGAIAII---DKSPRP 451

Query: 456 GEKF-GTFPMTAIGSKDGAEVLSYVKSSGNPVATILPSVTIINYKPAPAIAYFSSRGPNP 515
              F    P + + +KD   ++SY++S  +P A +L + TI N + +P IA FSSRGPN 
Sbjct: 452 DVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFN-RTSPVIASFSSRGPNT 511

Query: 516 KVLNIMKPDISAPGVNILAAWLGNDSSSTPQGTKSPLFNMISGTSMSCPHVSGVVAFVKS 575
             ++I+KPDI+APGV ILAA+  N   S    T+   +++ SGTSM+CPHV+GV A+VK+
Sbjct: 512 IAVDILKPDITAPGVEILAAFSPNGEPS-EDDTRRVKYSVFSGTSMACPHVAGVAAYVKT 551

Query: 576 QNPTWSPSAIKSAIMTT 583
             P WSPS I+SAIMTT
Sbjct: 572 FYPRWSPSMIQSAIMTT 551

BLAST of Lag0036767 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 9.2e-118
Identity = 258/599 (43.07%), Postives = 353/599 (58.93%), Query Frame = 0

Query: 16  FFLLSENGEIAAAAQGGNNG--VYIVYMG-------SASGSRTDFLRLLNSVNRNRNAVV 75
           FF L  +  +A+     ++G  +YIVYMG       SA       L  +        +V+
Sbjct: 11  FFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVVGSTFAPESVL 70

Query: 76  HTYKHGFTGFAARMSEQEAEAMRHRPGVVSVFRDPLLKLHTTHSWDFLISQTSVKIDANP 135
           HTYK  F GFA +++E+EAE +    GVVSVF + + +LHTT SWDFL    +V      
Sbjct: 71  HTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFPLTV------ 130

Query: 136 KSDPPSSSSQPYDTIIGILDTGIWPESESFNDKGMKPIPSRWKGTCMVGNDFTSSNCNKK 195
               P  S    + ++G+LDTGIWPES SF+D+G  P P +WKGTC   N+F    CN+K
Sbjct: 131 ----PRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNF---RCNRK 190

Query: 196 IIGARFYESSE--SDGIQYHSPRDEAGHGTHVASTAAGSAVANASYYGLAAGTAKGGSPG 255
           IIGAR Y      S G   + PRD  GHGTH ASTAAG  V+ A+ YGL  GTA+GG P 
Sbjct: 191 IIGARSYHIGRPISPG-DVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPL 250

Query: 256 SRIAMYRVCMADGCRGSAIMAAFDDAIADGVDCLSLSLG---TPYYFKQGLKDDPIAIGA 315
           +RIA Y+VC  DGC  + I+AA+DDAIADGVD +SLS+G     +YF      D IAIG+
Sbjct: 251 ARIAAYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFV-----DAIAIGS 310

Query: 316 FHAVEKGIVVVCSAGNDGPTSGSVVNDAPWILTVAASTIDRVFESDVVLGNKKVIKGEGI 375
           FHAVE+GI+   SAGN GP   +  + +PW+L+VAAST+DR F + V +GN +  +G  I
Sbjct: 311 FHAVERGILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSI 370

Query: 376 NFSALKKSPVYPLIQGKSAKKGDASEDSARICSEDSMDEALVKGKIVICESNVEGGGSNW 435
           N      +  YPL+ G+        + ++R C++ S++  L+KGKIV+CE++        
Sbjct: 371 N---TFDNQYYPLVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCEASF------- 430

Query: 436 QGQAETVKSLGGIGVVLIDDQSKLIGEKFGTFPMTAIGSKDGAEVLSYVKSSGNPVATIL 495
            G  E  KSL G   VL+   ++   + +   P + +   D    L Y+ S  +P ATI 
Sbjct: 431 -GPHEFFKSLDGAAGVLMTSNTRDYADSY-PLPSSVLDPNDLLATLRYIYSIRSPGATIF 490

Query: 496 PSVTIINYKPAPAIAYFSSRGPNPKVLNIMKPDISAPGVNILAAWLGNDSSSTPQG--TK 555
            S TI+N   AP +  FSSRGPN    +++KPDIS PGV ILAAW     S  P G   +
Sbjct: 491 KSTTILN-ASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAW----PSVAPVGGIRR 550

Query: 556 SPLFNMISGTSMSCPHVSGVVAFVKSQNPTWSPSAIKSAIMTTGKKLISAMKKKLFFSF 599
           + LFN+ISGTSMSCPH++G+  +VK+ NPTWSP+AIKSA+MTT   + +    +  F++
Sbjct: 551 NTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAY 573

BLAST of Lag0036767 vs. ExPASy TrEMBL
Match: A0A5D3E6D0 (CO(2)-response secreted protease isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G007880 PE=3 SV=1)

HSP 1 Score: 981.1 bits (2535), Expect = 2.1e-282
Identity = 501/586 (85.49%), Postives = 533/586 (90.96%), Query Frame = 0

Query: 1   MHSISFC-FFVLVCAIFFLLSENGEIAAAAQGGNNGVYIVYMGSASGSRTDFLRLLNSVN 60
           M SISFC F  LVC  F L+SEN E+A AA+   NGVYIVYMGSASGSR DFLRLLNSVN
Sbjct: 1   MQSISFCVFLALVCVTFCLVSENVEVADAAEDAKNGVYIVYMGSASGSRIDFLRLLNSVN 60

Query: 61  RNRNAVVHTYKHGFTGFAARMSEQEAEAMRHRPGVVSVFRDPLLKLHTTHSWDFLISQTS 120
           R RNA+VHTYKHGFTGFAAR+SE EA+AMR  PGVVSVF DP+LKLHTTHSWDFL+SQTS
Sbjct: 61  R-RNALVHTYKHGFTGFAARLSEHEAQAMRQSPGVVSVFPDPILKLHTTHSWDFLVSQTS 120

Query: 121 VKIDANPKSDPPSSSSQPYDTIIGILDTGIWPESESFNDKGMKPIPSRWKGTCMVGNDFT 180
           VK+DANPKSD P+SSSQPYDTIIGILDTGIWPESESFNDKGM PIPSRWKGTCM+G+DFT
Sbjct: 121 VKVDANPKSDSPTSSSQPYDTIIGILDTGIWPESESFNDKGMGPIPSRWKGTCMIGDDFT 180

Query: 181 SSNCNKKIIGARFYESSESDGIQYHSPRDEAGHGTHVASTAAGSAVANASYYGLAAGTAK 240
           SSNCN+KIIGARFYESSESDGI+YHSPRD AGHGTHVASTAAGSAVANASYYGLAAGTAK
Sbjct: 181 SSNCNRKIIGARFYESSESDGIRYHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAK 240

Query: 241 GGSPGSRIAMYRVCMADGCRGSAIMAAFDDAIADGVDCLSLSLGTPYYFKQGLKDDPIAI 300
           GGSP SRIAMYRVCMADGCRGS+I+ AFDD+IADGVD LSLSLG+P + +  L  DPIAI
Sbjct: 241 GGSPRSRIAMYRVCMADGCRGSSILKAFDDSIADGVDVLSLSLGSPSFLRPDLTADPIAI 300

Query: 301 GAFHAVEKGIVVVCSAGNDGPTSGSVVNDAPWILTVAASTIDRVFESDVVLGNKKVIKGE 360
           GAFHAVEKGI VVCSAGNDGP+SG+VVNDAPWILTVAASTIDR FESDVVLGNKKVIKGE
Sbjct: 301 GAFHAVEKGITVVCSAGNDGPSSGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGE 360

Query: 361 GINFSALKKSPVYPLIQGKSAKKGDASEDSARICSEDSMDEALVKGKIVICESNVEGGGS 420
           GINFS L+KSPVYPLIQGKSAKK  ASEDSARICSEDSMDEA VKGKIVICES+VEGGGS
Sbjct: 361 GINFSDLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDEAQVKGKIVICESSVEGGGS 420

Query: 421 NWQGQAETVKSLGGIGVVLIDDQSKLIGEKFGTFPMTAIGSKDGAEVLSYVKSSGNPVAT 480
           +WQ QAETVKSLGG+GVVLIDD SKL+ EKF T PMT I  KDG E+LSYV SS  PVAT
Sbjct: 421 DWQSQAETVKSLGGVGVVLIDDDSKLVAEKF-TTPMTVISKKDGPEILSYVNSSRKPVAT 480

Query: 481 ILPSVTIINYKPAPAIAYFSSRGPNPKVLNIMKPDISAPGVNILAAWLGNDSSSTPQGTK 540
           ILP+ TIINYKPAPAI YFSSRGPNP VLNI+KPDISAPGVNILAAWLGNDSSSTPQ TK
Sbjct: 481 ILPTETIINYKPAPAITYFSSRGPNPAVLNIIKPDISAPGVNILAAWLGNDSSSTPQATK 540

Query: 541 SPLFNMISGTSMSCPHVSGVVAFVKSQNPTWSPSAIKSAIMTTGKK 586
           SPLFN+ISGTSMSCPHVSGVVA VKSQNPTWSPSAIKSAIMTTGK+
Sbjct: 541 SPLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIKSAIMTTGKE 584

BLAST of Lag0036767 vs. ExPASy TrEMBL
Match: A0A0A0LJ71 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G873800 PE=3 SV=1)

HSP 1 Score: 974.5 bits (2518), Expect = 2.0e-280
Identity = 499/584 (85.45%), Postives = 530/584 (90.75%), Query Frame = 0

Query: 1   MHSISFC-FFVLVCAIFFLLSENGEIAAAAQGGNNGVYIVYMGSA-SGSRTDFLRLLNSV 60
           M SISFC FF LVC  FFL+SEN ++A AA+   NGVYIVYMGSA SG RTDFLRLLNSV
Sbjct: 1   MQSISFCVFFALVCVTFFLVSENVKVADAAEDARNGVYIVYMGSASSGFRTDFLRLLNSV 60

Query: 61  NRNRNAVVHTYKHGFTGFAARMSEQEAEAMRHRPGVVSVFRDPLLKLHTTHSWDFLISQT 120
           NR RNAVVHTYKHGFTGFAA +SE EA+AMR  PGVVSVF DPLLKLHTTHSWDFL+SQT
Sbjct: 61  NR-RNAVVHTYKHGFTGFAAHLSEHEAQAMRQSPGVVSVFPDPLLKLHTTHSWDFLVSQT 120

Query: 121 SVKIDANPKSDPPSSSSQPYDTIIGILDTGIWPESESFNDKGMKPIPSRWKGTCMVGNDF 180
           SVKIDANPKSDPP+SSSQPYDTIIGILDTGIWPESESFND GM PIPSRWKGTCM G+DF
Sbjct: 121 SVKIDANPKSDPPASSSQPYDTIIGILDTGIWPESESFNDMGMGPIPSRWKGTCMTGDDF 180

Query: 181 TSSNCNKKIIGARFYESSESDGIQYHSPRDEAGHGTHVASTAAGSAVANASYYGLAAGTA 240
           TSSNCN+KIIGARFYESSESDGI+YHSPRD AGHGTHVASTAAGSAVANASYYGLAAGTA
Sbjct: 181 TSSNCNRKIIGARFYESSESDGIRYHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTA 240

Query: 241 KGGSPGSRIAMYRVCMADGCRGSAIMAAFDDAIADGVDCLSLSLGTPYYFKQGLKDDPIA 300
           KGGSPGSRIAMYRVCMADGCRGS+IM AFDD+IADGVD LSLSLGTP  F+  L  DPIA
Sbjct: 241 KGGSPGSRIAMYRVCMADGCRGSSIMKAFDDSIADGVDVLSLSLGTPSVFRPDLTADPIA 300

Query: 301 IGAFHAVEKGIVVVCSAGNDGPTSGSVVNDAPWILTVAASTIDRVFESDVVLGNKKVIKG 360
           IGAFHAVEKGI VVCSAGNDGP+SG+VVNDAPWILTVAASTIDR FESDVVLGNKKVIKG
Sbjct: 301 IGAFHAVEKGITVVCSAGNDGPSSGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKG 360

Query: 361 EGINFSALKKSPVYPLIQGKSAKKGDASEDSARICSEDSMDEALVKGKIVICESNVEGGG 420
           EGINFS L+KSPVYPLI+GKSAKK   SEDSARICSEDSMDEA VKGKIVICE++VEGGG
Sbjct: 361 EGINFSDLQKSPVYPLIEGKSAKKASDSEDSARICSEDSMDEAQVKGKIVICENSVEGGG 420

Query: 421 SNWQGQAETVKSLGGIGVVLIDDQSKLIGEKFGTFPMTAIGSKDGAEVLSYVKSSGNPVA 480
           S+WQ QAETVK+LGG+G+VLIDD SKL+ EKF T PMT I  KDG E+LSYV SS  PVA
Sbjct: 421 SDWQSQAETVKNLGGVGLVLIDDDSKLVAEKFST-PMTVISKKDGLEILSYVNSSRKPVA 480

Query: 481 TILPSVTIINYKPAPAIAYFSSRGPNPKVLNIMKPDISAPGVNILAAWLGNDSSSTPQGT 540
           T+LP+ TIINYKPAPAI YFSSRGPNP VLNI+KPDISAPGVNILAAWLGNDSSSTPQ T
Sbjct: 481 TVLPTETIINYKPAPAITYFSSRGPNPAVLNIIKPDISAPGVNILAAWLGNDSSSTPQAT 540

Query: 541 KSPLFNMISGTSMSCPHVSGVVAFVKSQNPTWSPSAIKSAIMTT 583
           KSPLFN+ISGTSMSCPHVSGVVA VKSQNPTWSPSAI+SAIMTT
Sbjct: 541 KSPLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIRSAIMTT 582

BLAST of Lag0036767 vs. ExPASy TrEMBL
Match: A0A6J1IHT1 (CO(2)-response secreted protease OS=Cucurbita maxima OX=3661 GN=LOC111476411 PE=3 SV=1)

HSP 1 Score: 961.8 bits (2485), Expect = 1.3e-276
Identity = 494/583 (84.73%), Postives = 529/583 (90.74%), Query Frame = 0

Query: 1   MHSISFCFFVLVCAIFFLLSENGEIAAAAQGGNNGVYIVYMGSASGSRTDFLRLLNSVN- 60
           M +IS C F+LVC IF LLSEN +IAAAA  GNNGVYIVYMGSA  SRTDFLRLL SVN 
Sbjct: 1   MQTISSCAFLLVCVIFLLLSENAKIAAAA-AGNNGVYIVYMGSA--SRTDFLRLLTSVNR 60

Query: 61  RNRNAVVHTYKHGFTGFAARMSEQEAEAMRHRPGVVSVFRDPLLKLHTTHSWDFLISQTS 120
           RN NAVV TYKHGFTGFAAR+SEQEA AMR RPGVVSVF DP+LKLHTTHSWDFL+SQTS
Sbjct: 61  RNGNAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTS 120

Query: 121 VKIDANPKSDPPSSSSQPYDTIIGILDTGIWPESESFNDKGMKPIPSRWKGTCMVGNDFT 180
           VKID+ PKS+PP+SSSQP DTIIGILDTGIWPESESF D+GM PIPSRWKGTCM G+DFT
Sbjct: 121 VKIDSAPKSEPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFT 180

Query: 181 SSNCNKKIIGARFYESSESDGIQYHSPRDEAGHGTHVASTAAGSAVANASYYGLAAGTAK 240
           SSNCN+KIIGARFYESSESDGI++HSPRD AGHGTHVASTAAGSAVANASYYGLAAGTAK
Sbjct: 181 SSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAK 240

Query: 241 GGSPGSRIAMYRVCMADGCRGSAIMAAFDDAIADGVDCLSLSLGTPYYFKQGLKDDPIAI 300
           GGSPGSRIAMYRVCMADGC GS+I+AAFDDAI DGVD LSLSLGTPY++K  L  DPIAI
Sbjct: 241 GGSPGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGTPYFYKPELATDPIAI 300

Query: 301 GAFHAVEKGIVVVCSAGNDGPTSGSVVNDAPWILTVAASTIDRVFESDVVLGNKKVIKGE 360
           GAFHAVEKGI VVCSAGNDGPT GSVVNDAPWILTVAASTIDR FESDVVLGNKKVIKGE
Sbjct: 301 GAFHAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGE 360

Query: 361 GINFSALKKSPVYPLIQGKSAKKGDASEDSARICSEDSMDEALVKGKIVICESNVEGGGS 420
           GINFSAL+ SPVYPLI GKSA K   SEDSARICSE SMDEALVKGKIVICES+ EGGGS
Sbjct: 361 GINFSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGS 420

Query: 421 NWQGQAETVKSLGGIGVVLIDDQSKLIGEKFGTFPMTAIGSKDGAEVLSYVKSSGNPVAT 480
            WQGQAETV+SLGG+G+VLIDDQ+KL+ EKF T P+TAI SKDG EVL+YV SS NPVAT
Sbjct: 421 YWQGQAETVESLGGVGIVLIDDQAKLVAEKF-TSPITAISSKDGDEVLAYVHSSRNPVAT 480

Query: 481 ILPSVTIINYKPAPAIAYFSSRGPNPKVLNIMKPDISAPGVNILAAWLGNDSSSTPQGTK 540
           ILP++T+INYKPAPAIAYFSSRGPNP VLN++KPDISAPGVNILAAWLGND++STPQ   
Sbjct: 481 ILPTLTVINYKPAPAIAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPEN 540

Query: 541 SPLFNMISGTSMSCPHVSGVVAFVKSQNPTWSPSAIKSAIMTT 583
           SPLFN+ISGTSMSCPHVSGVVA VKSQNPTWSPSAIKSAIMTT
Sbjct: 541 SPLFNVISGTSMSCPHVSGVVAMVKSQNPTWSPSAIKSAIMTT 579

BLAST of Lag0036767 vs. ExPASy TrEMBL
Match: A0A6J1FFB5 (CO(2)-response secreted protease OS=Cucurbita moschata OX=3662 GN=LOC111443427 PE=3 SV=1)

HSP 1 Score: 960.7 bits (2482), Expect = 2.9e-276
Identity = 493/583 (84.56%), Postives = 528/583 (90.57%), Query Frame = 0

Query: 1   MHSISFCFFVLVCAIFFLLSENGEIAAAAQGGNNGVYIVYMGSASGSRTDFLRLLNSVN- 60
           M +IS C F+LVC IF LLSEN +IAAAA  GNNGVYIVYMGSA  SRTDFLRLL SVN 
Sbjct: 1   MQTISSCVFLLVCVIFLLLSENAKIAAAA-AGNNGVYIVYMGSA--SRTDFLRLLTSVNR 60

Query: 61  RNRNAVVHTYKHGFTGFAARMSEQEAEAMRHRPGVVSVFRDPLLKLHTTHSWDFLISQTS 120
           RN NAVV TYKHGFTGFAAR+SEQEA AMR RPGVVSVF DP+LKLHTTHSWDFL+SQTS
Sbjct: 61  RNGNAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTS 120

Query: 121 VKIDANPKSDPPSSSSQPYDTIIGILDTGIWPESESFNDKGMKPIPSRWKGTCMVGNDFT 180
           VKID+ PK+DPP+SSSQP+DTIIGILDTGIWPESESF D+GM PIPSRWKGTCM G+DFT
Sbjct: 121 VKIDSAPKADPPTSSSQPFDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFT 180

Query: 181 SSNCNKKIIGARFYESSESDGIQYHSPRDEAGHGTHVASTAAGSAVANASYYGLAAGTAK 240
           SSNCN+KIIGARFYESSESDGI++HSPRD AGHGTHVASTAAGSAVANASYYGLAAGTAK
Sbjct: 181 SSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAK 240

Query: 241 GGSPGSRIAMYRVCMADGCRGSAIMAAFDDAIADGVDCLSLSLGTPYYFKQGLKDDPIAI 300
           GGSPGSRIAMYRVCMADGC GS+I+AAFDDAI DGVD LSLSLG P Y+K  L  DPIAI
Sbjct: 241 GGSPGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPNYYKPELTTDPIAI 300

Query: 301 GAFHAVEKGIVVVCSAGNDGPTSGSVVNDAPWILTVAASTIDRVFESDVVLGNKKVIKGE 360
           GAFHAVEKGI VVCSAGNDGPT GSVVNDAPWILTVAASTIDR FESDVVLGNKKVIKGE
Sbjct: 301 GAFHAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGE 360

Query: 361 GINFSALKKSPVYPLIQGKSAKKGDASEDSARICSEDSMDEALVKGKIVICESNVEGGGS 420
           GINFSAL+ SPVYPLI GKSA K   SEDSARICSE SMDEALVKGKIVICES+ EGGGS
Sbjct: 361 GINFSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGS 420

Query: 421 NWQGQAETVKSLGGIGVVLIDDQSKLIGEKFGTFPMTAIGSKDGAEVLSYVKSSGNPVAT 480
            WQGQAETV+SLGG+G+VLIDDQ+KL+ EKF T P+TAI SKDG EVL+YV SS NPVAT
Sbjct: 421 YWQGQAETVESLGGVGIVLIDDQTKLVAEKF-TSPITAISSKDGDEVLAYVHSSRNPVAT 480

Query: 481 ILPSVTIINYKPAPAIAYFSSRGPNPKVLNIMKPDISAPGVNILAAWLGNDSSSTPQGTK 540
           ILP++T+INYKPAPAIAYFSSRGPNP VLN++KPDISAPGVNILAAWLGND++STPQ   
Sbjct: 481 ILPTLTVINYKPAPAIAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPEN 540

Query: 541 SPLFNMISGTSMSCPHVSGVVAFVKSQNPTWSPSAIKSAIMTT 583
           SPLFN+ISGTSMSCPHVSGVVA VKSQNPTWSPSAIKSAIMTT
Sbjct: 541 SPLFNVISGTSMSCPHVSGVVAMVKSQNPTWSPSAIKSAIMTT 579

BLAST of Lag0036767 vs. ExPASy TrEMBL
Match: A0A6J1C938 (CO(2)-response secreted protease OS=Momordica charantia OX=3673 GN=LOC111009346 PE=3 SV=1)

HSP 1 Score: 952.2 bits (2460), Expect = 1.0e-273
Identity = 484/584 (82.88%), Postives = 525/584 (89.90%), Query Frame = 0

Query: 1   MHSISFCFFVLVCAIFFLLSENGEIAAAAQGGNNGVYIVYMGSASGSRTDFLRLLNSVN- 60
           M  I +    L  ++ FL+S +G+   AA+ GNNGVYIVYMG+AS SRTDFLRLL+SVN 
Sbjct: 1   MQRIIYFSVFLFASVIFLVSVHGK--TAAEAGNNGVYIVYMGTASASRTDFLRLLSSVNR 60

Query: 61  RNRNAVVHTYKHGFTGFAARMSEQEAEAMRHRPGVVSVFRDPLLKLHTTHSWDFLISQTS 120
           RNRNAVVHTYKHGF+GFAAR+SE+E + MR  PGVVSVF DPLLKLHTTHSWDFL+SQTS
Sbjct: 61  RNRNAVVHTYKHGFSGFAARLSEKETQEMRQTPGVVSVFPDPLLKLHTTHSWDFLVSQTS 120

Query: 121 VKIDANP-KSDPPSSSSQPYDTIIGILDTGIWPESESFNDKGMKPIPSRWKGTCMVGNDF 180
           VKIDANP KSDPP+SSSQPYD+IIGILDTGIWPESESFND GM PIP RWKGTCMVGNDF
Sbjct: 121 VKIDANPTKSDPPASSSQPYDSIIGILDTGIWPESESFNDNGMGPIPLRWKGTCMVGNDF 180

Query: 181 TSSNCNKKIIGARFYESSESDGIQYHSPRDEAGHGTHVASTAAGSAVANASYYGLAAGTA 240
           TSSNCN K+IGARFYESS+SD I++HSPRDEAGHGTHVASTAAGSA ANASYYGLAAGTA
Sbjct: 181 TSSNCNTKLIGARFYESSDSDEIRFHSPRDEAGHGTHVASTAAGSAAANASYYGLAAGTA 240

Query: 241 KGGSPGSRIAMYRVCMADGCRGSAIMAAFDDAIADGVDCLSLSLGTPYYFKQGLKDDPIA 300
           KGGSPGSRIAMYRVC  DGCRGSAIMAAFDDAIADGVD LSLSLG+PYYF+Q  KDDPIA
Sbjct: 241 KGGSPGSRIAMYRVCGTDGCRGSAIMAAFDDAIADGVDVLSLSLGSPYYFRQETKDDPIA 300

Query: 301 IGAFHAVEKGIVVVCSAGNDGPTSGSVVNDAPWILTVAASTIDRVFESDVVLGNKKVIKG 360
           IGAFHAVEKGIVVVCSAGNDGPTSGSVVNDAPWILTVAASTIDR FESDVVLGN KVIKG
Sbjct: 301 IGAFHAVEKGIVVVCSAGNDGPTSGSVVNDAPWILTVAASTIDRDFESDVVLGNNKVIKG 360

Query: 361 EGINFSALKKSPVYPLIQGKSAKKGDASEDSARICSEDSMDEALVKGKIVICESNVEGGG 420
           EGINFSALKK+P YPLIQGKSAKK DASEDSARICSEDSMDEALVKGKIVICES+VEGGG
Sbjct: 361 EGINFSALKKTPEYPLIQGKSAKKADASEDSARICSEDSMDEALVKGKIVICESSVEGGG 420

Query: 421 SNWQGQAETVKSLGGIGVVLIDDQSKLIGEKFGTFPMTAIGSKDGAEVLSYVKSSGNPVA 480
           S+WQ Q ETVK L GIG+VLIDD++KL+ EKF    +TAI  KD AEVLSY  SS NP A
Sbjct: 421 SDWQNQIETVKGLEGIGMVLIDDRAKLVAEKFDGSNITAISKKDSAEVLSYANSSRNPTA 480

Query: 481 TILPSVTIINYKPAPAIAYFSSRGPNPKVLNIMKPDISAPGVNILAAWLGNDSSSTPQGT 540
           TILP+VT+INYKPAPA+AYFSSRGPNP +LNI+KPDISAPGVNILAAWLGNDS+STPQG 
Sbjct: 481 TILPTVTVINYKPAPAVAYFSSRGPNPAILNIIKPDISAPGVNILAAWLGNDSNSTPQGK 540

Query: 541 KSPLFNMISGTSMSCPHVSGVVAFVKSQNPTWSPSAIKSAIMTT 583
           K PLFN+ISGTSMSCPHVSGVV  ++S+NP+WSPSAIKSAIMTT
Sbjct: 541 KPPLFNVISGTSMSCPHVSGVVGLLRSKNPSWSPSAIKSAIMTT 582

BLAST of Lag0036767 vs. TAIR 10
Match: AT1G20160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 685.3 bits (1767), Expect = 4.6e-197
Identity = 356/579 (61.49%), Postives = 440/579 (75.99%), Query Frame = 0

Query: 8   FFVLVCAIFFLLSENGEIAAAAQGGNNGVYIVYMGSASGSRTDFLR--LLNSV-NRNRNA 67
           F  L+C +F   +E      A     +GVYIVYMGSAS +        L+N++  R  N 
Sbjct: 13  FLYLLCILFMTETE------AGSRNGDGVYIVYMGSASSAANANRAQILINTMFKRRAND 72

Query: 68  VVHTYKHGFTGFAARMSEQEAEAMRHRPGVVSVFRDPLLKLHTTHSWDFLISQTSVKIDA 127
           ++HTYKHGF+GFAAR++ +EA+ +  +PGVVSVF DP  +LHTTHSWDFL  QTSVK+D+
Sbjct: 73  LLHTYKHGFSGFAARLTAEEAKVIAKKPGVVSVFPDPHFQLHTTHSWDFLKYQTSVKVDS 132

Query: 128 NPKSDPPSSSSQPYDTIIGILDTGIWPESESFNDKGMKPIPSRWKGTCMVGNDFTSSNCN 187
            P   P S+S   YD+I+GILDTGIWPESESFNDK M PIPSRWKGTCM   DF SSNCN
Sbjct: 133 GP---PSSASDGSYDSIVGILDTGIWPESESFNDKDMGPIPSRWKGTCMEAKDFKSSNCN 192

Query: 188 KKIIGARFYESSESDGIQYHSPRDEAGHGTHVASTAAGSAVANASYYGLAAGTAKGGSPG 247
           +KIIGAR+Y++ + D  +Y++ RD  GHG+HV+ST AGSAV NASYYG+A+GTAKGGS  
Sbjct: 193 RKIIGARYYKNPDDDS-EYYTTRDVIGHGSHVSSTIAGSAVENASYYGVASGTAKGGSQN 252

Query: 248 SRIAMYRVCMADGCRGSAIMAAFDDAIADGVDCLSLSLGTPYYFKQGLKDDPIAIGAFHA 307
           +RIAMY+VC   GC GS+I+AAFDDAIADGVD LSLSLG P Y +  L  DPIAIGAFHA
Sbjct: 253 ARIAMYKVCNPGGCTGSSILAAFDDAIADGVDVLSLSLGAPAYARIDLNTDPIAIGAFHA 312

Query: 308 VEKGIVVVCSAGNDGPTSGSVVNDAPWILTVAASTIDRVFESDVVLGNKKVIKGEGINFS 367
           VE+GI+V+CSAGNDGP  G+V N APWI+TVAA+TIDR FESDVVLG  KVIKGEGI+FS
Sbjct: 313 VEQGILVICSAGNDGPDGGTVTNTAPWIMTVAANTIDRDFESDVVLGGNKVIKGEGIHFS 372

Query: 368 ALKKSPVYPLIQGKSAKKGDASEDSARICSEDSMDEALVKGKIVICESNVEGGGSNWQGQ 427
            + KSPVYPLI GKSAK  DASE SAR C  DS+D+  VKGKIV+CE NV  GGS +   
Sbjct: 373 NVSKSPVYPLIHGKSAKSADASEGSARACDSDSLDQEKVKGKIVLCE-NV--GGSYYASS 432

Query: 428 A-ETVKSLGGIGVVLIDDQSKLIGEKFGTFPMTAIGSKDGAEVLSYVKSSGNPVATILPS 487
           A + VKS GG G V +DD+++ +   +G+FP T I SK+ AE+ SY+ S+ +PVATILP+
Sbjct: 433 ARDEVKSKGGTGCVFVDDRTRAVASAYGSFPTTVIDSKEAAEIFSYLNSTKDPVATILPT 492

Query: 488 VTIINYKPAPAIAYFSSRGPNPKVLNIMKPDISAPGVNILAAWLGNDSSSTPQGTKSPLF 547
            T+  + PAPA+AYFSSRGP+    +I+KPDI+APGV+ILAAW GNDSS + +G  +  +
Sbjct: 493 ATVEKFTPAPAVAYFSSRGPSSLTRSILKPDITAPGVSILAAWTGNDSSISLEGKPASQY 552

Query: 548 NMISGTSMSCPHVSGVVAFVKSQNPTWSPSAIKSAIMTT 583
           N+ISGTSM+ PHVS V + +KSQ+PTW PSAI+SAIMTT
Sbjct: 553 NVISGTSMAAPHVSAVASLIKSQHPTWGPSAIRSAIMTT 578

BLAST of Lag0036767 vs. TAIR 10
Match: AT1G20160.2 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 670.2 bits (1728), Expect = 1.5e-192
Identity = 344/546 (63.00%), Postives = 424/546 (77.66%), Query Frame = 0

Query: 41  MGSASGSRTDFLR--LLNSV-NRNRNAVVHTYKHGFTGFAARMSEQEAEAMRHRPGVVSV 100
           MGSAS +        L+N++  R  N ++HTYKHGF+GFAAR++ +EA+ +  +PGVVSV
Sbjct: 1   MGSASSAANANRAQILINTMFKRRANDLLHTYKHGFSGFAARLTAEEAKVIAKKPGVVSV 60

Query: 101 FRDPLLKLHTTHSWDFLISQTSVKIDANPKSDPPSSSSQPYDTIIGILDTGIWPESESFN 160
           F DP  +LHTTHSWDFL  QTSVK+D+ P   P S+S   YD+I+GILDTGIWPESESFN
Sbjct: 61  FPDPHFQLHTTHSWDFLKYQTSVKVDSGP---PSSASDGSYDSIVGILDTGIWPESESFN 120

Query: 161 DKGMKPIPSRWKGTCMVGNDFTSSNCNKKIIGARFYESSESDGIQYHSPRDEAGHGTHVA 220
           DK M PIPSRWKGTCM   DF SSNCN+KIIGAR+Y++ + D  +Y++ RD  GHG+HV+
Sbjct: 121 DKDMGPIPSRWKGTCMEAKDFKSSNCNRKIIGARYYKNPDDDS-EYYTTRDVIGHGSHVS 180

Query: 221 STAAGSAVANASYYGLAAGTAKGGSPGSRIAMYRVCMADGCRGSAIMAAFDDAIADGVDC 280
           ST AGSAV NASYYG+A+GTAKGGS  +RIAMY+VC   GC GS+I+AAFDDAIADGVD 
Sbjct: 181 STIAGSAVENASYYGVASGTAKGGSQNARIAMYKVCNPGGCTGSSILAAFDDAIADGVDV 240

Query: 281 LSLSLGTPYYFKQGLKDDPIAIGAFHAVEKGIVVVCSAGNDGPTSGSVVNDAPWILTVAA 340
           LSLSLG P Y +  L  DPIAIGAFHAVE+GI+V+CSAGNDGP  G+V N APWI+TVAA
Sbjct: 241 LSLSLGAPAYARIDLNTDPIAIGAFHAVEQGILVICSAGNDGPDGGTVTNTAPWIMTVAA 300

Query: 341 STIDRVFESDVVLGNKKVIKGEGINFSALKKSPVYPLIQGKSAKKGDASEDSARICSEDS 400
           +TIDR FESDVVLG  KVIKGEGI+FS + KSPVYPLI GKSAK  DASE SAR C  DS
Sbjct: 301 NTIDRDFESDVVLGGNKVIKGEGIHFSNVSKSPVYPLIHGKSAKSADASEGSARACDSDS 360

Query: 401 MDEALVKGKIVICESNVEGGGSNWQGQA-ETVKSLGGIGVVLIDDQSKLIGEKFGTFPMT 460
           +D+  VKGKIV+CE NV  GGS +   A + VKS GG G V +DD+++ +   +G+FP T
Sbjct: 361 LDQEKVKGKIVLCE-NV--GGSYYASSARDEVKSKGGTGCVFVDDRTRAVASAYGSFPTT 420

Query: 461 AIGSKDGAEVLSYVKSSGNPVATILPSVTIINYKPAPAIAYFSSRGPNPKVLNIMKPDIS 520
            I SK+ AE+ SY+ S+ +PVATILP+ T+  + PAPA+AYFSSRGP+    +I+KPDI+
Sbjct: 421 VIDSKEAAEIFSYLNSTKDPVATILPTATVEKFTPAPAVAYFSSRGPSSLTRSILKPDIT 480

Query: 521 APGVNILAAWLGNDSSSTPQGTKSPLFNMISGTSMSCPHVSGVVAFVKSQNPTWSPSAIK 580
           APGV+ILAAW GNDSS + +G  +  +N+ISGTSM+ PHVS V + +KSQ+PTW PSAI+
Sbjct: 481 APGVSILAAWTGNDSSISLEGKPASQYNVISGTSMAAPHVSAVASLIKSQHPTWGPSAIR 539

Query: 581 SAIMTT 583
           SAIMTT
Sbjct: 541 SAIMTT 539

BLAST of Lag0036767 vs. TAIR 10
Match: AT1G20150.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 564.7 bits (1454), Expect = 9.0e-161
Identity = 305/594 (51.35%), Postives = 417/594 (70.20%), Query Frame = 0

Query: 3   SISFCFFVLVCAIFFLLSENGEIAAAAQGGNNGVYIVYMGSAS---GSRTDFLRLLNS-V 62
           +I+  FF     +FF LS   +    ++   +G YI+YMG+AS    +  D + LL+S +
Sbjct: 6   TITIMFF-----MFFFLSVIQK--CKSETSKSGDYIIYMGAASSDGSTDNDHVELLSSLL 65

Query: 63  NRNRNAVVHTYKHGFTGFAARMSEQEAEAMRHRPGVVSVFRDPLLKLHTTHSWDFLISQT 122
            R+    +H YKHGF+GFAA +SE EA  +  +PGV+SVF D +L+LHTT SWDFL+ Q 
Sbjct: 66  QRSGKTPMHRYKHGFSGFAAHLSEDEAHLIAKQPGVLSVFPDQMLQLHTTRSWDFLV-QE 125

Query: 123 SVKIDA--NPKSDPPSSSSQPYDTIIGILDTGIWPESESFNDKGMKPIPSRWKGTCMVGN 182
           S + D      +    S     DTIIG LD+GIWPE++SFND+ M P+P +WKGTCM G 
Sbjct: 126 SYQRDTYFTEMNYEQESEMHEGDTIIGFLDSGIWPEAQSFNDRHMGPVPEKWKGTCMRGK 185

Query: 183 DF--TSSNCNKKIIGARFYESSESDGIQYHSPRDEAGHGTHVASTAAGSAVANASYYGLA 242
                S  CN+K+IGAR+Y SS      Y +PRD  GHGTHVAS AAG  +ANASYYGLA
Sbjct: 186 KTQPDSFRCNRKLIGARYYNSSFFLDPDYETPRDFLGHGTHVASIAAGQIIANASYYGLA 245

Query: 243 AGTAKGGSPGSRIAMYRVCMADGCRGSAIMAAFDDAIADGVDCLSLSLGTPYYFKQGLKD 302
           +G  +GGSP SRIAMYR C   GCRGS+I+AAFDDAIADGVD +S+S+G    +   L +
Sbjct: 246 SGIMRGGSPSSRIAMYRACSLLGCRGSSILAAFDDAIADGVDVISISMG---LWPDNLLE 305

Query: 303 DPIAIGAFHAVEKGIVVVCSAGNDGPTSGSVVNDAPWILTVAASTIDRVFESDVVLG--N 362
           DP++IG+FHAVE+GI VVCS GN GP+S SV N APW++TVAASTIDR FES+++LG   
Sbjct: 306 DPLSIGSFHAVERGITVVCSVGNSGPSSQSVFNAAPWMITVAASTIDRGFESNILLGGDE 365

Query: 363 KKVIKGEGINFSALKKSPVYPLIQGKSAKKGDASEDSARICSEDSMDEALVKGKIVICES 422
            ++I+G GIN + + K+  YPLI  +SAKK DA+E++AR C+ D++D+ +VKGKIV+C+S
Sbjct: 366 NRLIEGFGINIANIDKTQAYPLIHARSAKKIDANEEAARNCAPDTLDQTIVKGKIVVCDS 425

Query: 423 NVEGGGSNWQGQAETVKSLGGIGVVLIDDQSKLIGEKFGTFPMTAIGSKDGAEVLSYVKS 482
           +++     W  +++ VK LGGIG+VL+DD+S  +     +F +T I  +DG +++SY+ S
Sbjct: 426 DLDNQVIQW--KSDEVKRLGGIGMVLVDDESMDLSFIDPSFLVTIIKPEDGIQIMSYINS 485

Query: 483 SGNPVATILPSVTIINYKPAPAIAYFSSRGPNPKVLNIMKPDISAPGVNILAAWLGNDSS 542
           +  P+ATI+P+ +   +  AP+I  FSSRGP     +I+KPDI+APGVNILA+WL  D +
Sbjct: 486 TREPIATIMPTRSRTGHMLAPSIPSFSSRGPYLLTRSILKPDIAAPGVNILASWLVGDRN 545

Query: 543 STPQGTKSPLFNMISGTSMSCPHVSGVVAFVKSQNPTWSPSAIKSAIMTTGKKL 587
           + P+G   PLFN+ SGTSMSCPHVSG+ A +KS+ P+WSP+AI+SAIMTT  ++
Sbjct: 546 AAPEGKPPPLFNIESGTSMSCPHVSGIAARLKSRYPSWSPAAIRSAIMTTAVQM 586

BLAST of Lag0036767 vs. TAIR 10
Match: AT5G59090.2 (subtilase 4.12 )

HSP 1 Score: 435.6 bits (1119), Expect = 6.3e-122
Identity = 258/559 (46.15%), Postives = 348/559 (62.25%), Query Frame = 0

Query: 36  VYIVYMGSASGSRTDF---------LRLLNSVNRNRNAVVHTYKHGFTGFAARMSEQEAE 95
           VYIVYMGS S SR D+         L+ +   +     +V +YK  F GFAAR++E E  
Sbjct: 32  VYIVYMGSLS-SRADYIPTSDHMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERT 91

Query: 96  AMRHRPGVVSVFRDPLLKLHTTHSWDFLISQTSVKIDANPKSDPPSSSSQPYDTIIGILD 155
            +    GVVSVF + +L+LHTT SWDF+     VK   N K +    S    DTIIG++D
Sbjct: 92  LIAEIEGVVSVFPNKILQLHTTTSWDFM----GVKEGKNTKRNLAIES----DTIIGVID 151

Query: 156 TGIWPESESFNDKGMKPIPSRWKGTCMVGNDFTSSNCNKKIIGARFYESSESDGIQYHSP 215
           TGIWPES+SF+DKG  P P +WKG C  G +FT   CN K+IGAR Y S           
Sbjct: 152 TGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFT---CNNKLIGARDYTS--------EGT 211

Query: 216 RDEAGHGTHVASTAAGSAVANASYYGLAAGTAKGGSPGSRIAMYRVCMADGCRGSAIMAA 275
           RD +GHGTH ASTAAG+AV + S++G+  GT +GG P SRIA Y+VC   GC   A++++
Sbjct: 212 RDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALLSS 271

Query: 276 FDDAIADGVDCLSLSLGTPYYFKQGLKDDPIAIGAFHAVEKGIVVVCSAGNDGPTSGSVV 335
           FDDAIADGVD +++S+G  + F    +DDPIAIGAFHA+ KGI+ V SAGN GP   +V 
Sbjct: 272 FDDAIADGVDLITISIG--FQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVS 331

Query: 336 NDAPWILTVAASTIDRVFESDVVLGNKKVIKGEGINFSALKKSPVYPLIQGKSAKKGDAS 395
           + APWI TVAAST +R F + VVLGN K + G  +N   + K   YPL+ GKSA      
Sbjct: 332 HVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDM-KGKKYPLVYGKSAASSACD 391

Query: 396 EDSARICSEDSMDEALVKGKIVICESNVEGGGSNWQGQAETVKSLGGIGVVLIDDQSKLI 455
             +A +C+   ++++ VKGKI++C     GG S +    +  KS+G I ++   D+S   
Sbjct: 392 AKTAALCAPACLNKSRVKGKILVC-----GGPSGY----KIAKSVGAIAII---DKSPRP 451

Query: 456 GEKF-GTFPMTAIGSKDGAEVLSYVKSSGNPVATILPSVTIINYKPAPAIAYFSSRGPNP 515
              F    P + + +KD   ++SY++S  +P A +L + TI N + +P IA FSSRGPN 
Sbjct: 452 DVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFN-RTSPVIASFSSRGPNT 511

Query: 516 KVLNIMKPDISAPGVNILAAWLGNDSSSTPQGTKSPLFNMISGTSMSCPHVSGVVAFVKS 575
             ++I+KPDI+APGV ILAA+  N   S    T+   +++ SGTSM+CPHV+GV A+VK+
Sbjct: 512 IAVDILKPDITAPGVEILAAFSPNGEPS-EDDTRRVKYSVFSGTSMACPHVAGVAAYVKT 553

Query: 576 QNPTWSPSAIKSAIMTTGK 585
             P WSPS I+SAIMTT K
Sbjct: 572 FYPRWSPSMIQSAIMTTAK 553

BLAST of Lag0036767 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 433.7 bits (1114), Expect = 2.4e-121
Identity = 257/557 (46.14%), Postives = 347/557 (62.30%), Query Frame = 0

Query: 36  VYIVYMGSASGSRTDF---------LRLLNSVNRNRNAVVHTYKHGFTGFAARMSEQEAE 95
           VYIVYMGS S SR D+         L+ +   +     +V +YK  F GFAAR++E E  
Sbjct: 32  VYIVYMGSLS-SRADYIPTSDHMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERT 91

Query: 96  AMRHRPGVVSVFRDPLLKLHTTHSWDFLISQTSVKIDANPKSDPPSSSSQPYDTIIGILD 155
            +    GVVSVF + +L+LHTT SWDF+     VK   N K +    S    DTIIG++D
Sbjct: 92  LIAEIEGVVSVFPNKILQLHTTTSWDFM----GVKEGKNTKRNLAIES----DTIIGVID 151

Query: 156 TGIWPESESFNDKGMKPIPSRWKGTCMVGNDFTSSNCNKKIIGARFYESSESDGIQYHSP 215
           TGIWPES+SF+DKG  P P +WKG C  G +FT   CN K+IGAR Y S           
Sbjct: 152 TGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFT---CNNKLIGARDYTS--------EGT 211

Query: 216 RDEAGHGTHVASTAAGSAVANASYYGLAAGTAKGGSPGSRIAMYRVCMADGCRGSAIMAA 275
           RD +GHGTH ASTAAG+AV + S++G+  GT +GG P SRIA Y+VC   GC   A++++
Sbjct: 212 RDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALLSS 271

Query: 276 FDDAIADGVDCLSLSLGTPYYFKQGLKDDPIAIGAFHAVEKGIVVVCSAGNDGPTSGSVV 335
           FDDAIADGVD +++S+G  + F    +DDPIAIGAFHA+ KGI+ V SAGN GP   +V 
Sbjct: 272 FDDAIADGVDLITISIG--FQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVS 331

Query: 336 NDAPWILTVAASTIDRVFESDVVLGNKKVIKGEGINFSALKKSPVYPLIQGKSAKKGDAS 395
           + APWI TVAAST +R F + VVLGN K + G  +N   + K   YPL+ GKSA      
Sbjct: 332 HVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDM-KGKKYPLVYGKSAASSACD 391

Query: 396 EDSARICSEDSMDEALVKGKIVICESNVEGGGSNWQGQAETVKSLGGIGVVLIDDQSKLI 455
             +A +C+   ++++ VKGKI++C     GG S +    +  KS+G I ++   D+S   
Sbjct: 392 AKTAALCAPACLNKSRVKGKILVC-----GGPSGY----KIAKSVGAIAII---DKSPRP 451

Query: 456 GEKF-GTFPMTAIGSKDGAEVLSYVKSSGNPVATILPSVTIINYKPAPAIAYFSSRGPNP 515
              F    P + + +KD   ++SY++S  +P A +L + TI N + +P IA FSSRGPN 
Sbjct: 452 DVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFN-RTSPVIASFSSRGPNT 511

Query: 516 KVLNIMKPDISAPGVNILAAWLGNDSSSTPQGTKSPLFNMISGTSMSCPHVSGVVAFVKS 575
             ++I+KPDI+APGV ILAA+  N   S    T+   +++ SGTSM+CPHV+GV A+VK+
Sbjct: 512 IAVDILKPDITAPGVEILAAFSPNGEPS-EDDTRRVKYSVFSGTSMACPHVAGVAAYVKT 551

Query: 576 QNPTWSPSAIKSAIMTT 583
             P WSPS I+SAIMTT
Sbjct: 572 FYPRWSPSMIQSAIMTT 551

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYK31472.14.3e-28285.49CO(2)-response secreted protease isoform X2 [Cucumis melo var. makuwa][more]
XP_004136374.14.0e-28085.45CO(2)-response secreted protease [Cucumis sativus] >KGN60051.1 hypothetical prot... [more]
XP_038896398.16.9e-28085.67CO(2)-response secreted protease [Benincasa hispida][more]
XP_022975820.12.7e-27684.73CO(2)-response secreted protease [Cucurbita maxima][more]
XP_022937005.16.0e-27684.56CO(2)-response secreted protease [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9LNU16.4e-19661.49CO(2)-response secreted protease OS=Arabidopsis thaliana OX=3702 GN=CRSP PE=2 SV... [more]
F4HSQ21.3e-15951.35Subtilisin-like protease SBT5.1 OS=Arabidopsis thaliana OX=3702 GN=SBT5.1 PE=3 S... [more]
A9JQS72.8e-12745.69Subtilisin-like serine-protease S OS=Lotus japonicus OX=34305 GN=SbtS PE=2 SV=1[more]
Q8L7D23.4e-12046.14Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2... [more]
Q395479.2e-11843.07Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3E6D02.1e-28285.49CO(2)-response secreted protease isoform X2 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A0A0LJ712.0e-28085.45Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G873800 PE=3 SV=1[more]
A0A6J1IHT11.3e-27684.73CO(2)-response secreted protease OS=Cucurbita maxima OX=3661 GN=LOC111476411 PE=... [more]
A0A6J1FFB52.9e-27684.56CO(2)-response secreted protease OS=Cucurbita moschata OX=3662 GN=LOC111443427 P... [more]
A0A6J1C9381.0e-27382.88CO(2)-response secreted protease OS=Momordica charantia OX=3673 GN=LOC111009346 ... [more]
Match NameE-valueIdentityDescription
AT1G20160.14.6e-19761.49Subtilisin-like serine endopeptidase family protein [more]
AT1G20160.21.5e-19263.00Subtilisin-like serine endopeptidase family protein [more]
AT1G20150.19.0e-16151.35Subtilisin-like serine endopeptidase family protein [more]
AT5G59090.26.3e-12246.15subtilase 4.12 [more]
AT5G59090.12.4e-12146.14subtilase 4.12 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 547..563
score: 52.12
coord: 208..221
score: 53.07
coord: 137..156
score: 26.21
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 36..106
e-value: 2.6E-12
score: 47.2
NoneNo IPR availableGENE3D3.50.30.30coord: 341..487
e-value: 2.7E-163
score: 546.1
NoneNo IPR availablePANTHERPTHR10795:SF598CO(2)-RESPONSE SECRETED PROTEASEcoord: 58..583
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 110..604
score: 26.577251
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 347..480
e-value: 2.64867E-25
score: 99.4103
NoneNo IPR availableSUPERFAMILY54897Protease propeptides/inhibitorscoord: 36..102
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 140..586
e-value: 2.2E-45
score: 155.1
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 20..106
e-value: 2.3E-18
score: 68.4
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 139..589
e-value: 2.7E-163
score: 546.1
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 135..586
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 58..583
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 548..558
IPR022398Peptidase S8, subtilisin, His-active sitePROSITEPS00137SUBTILASE_HIScoord: 212..222
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 103..582
e-value: 1.27681E-131
score: 385.798

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0036767.1Lag0036767.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity