Lag0036432 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0036432
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCalmodulin-binding protein 60 G-like
Locationchr3: 46407289 .. 46410989 (+)
RNA-Seq ExpressionLag0036432
SyntenyLag0036432
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAGTGCTACAAAAAGAAACTTTCCAAATTTTCACAAGGTTGAAGAAGATGAAGATGAAGATGATCACCCTTCTCAAACTAACCCTAAAAGAATCTGCAACTCTACTGGGTTTGCTGTCTCTCTCTCTTTCTTTTTCTTCTTCTTCTTCTCTTCCCCTTTTTCAAGAAATGGGTTTCTTGATTTTGCCAGTTATGAAGCTGAATCTGACATTGGATCAGCTGAGCTTTTCTCTGTTGAATCTGAATCACACTCTCAAATAGAATCTTTCCTTCGTCGAATGGTATTTTTCGGGTCATTTTGGAATCATTTTCTAAATGTTTAGAAATACTTTCTTAAACATTTCAACCCCTCTTGATGACCATTTGGTTTTTGGTTTTTGGTTTTTGAAAATTAGGTTTGTTTTCTTCCATTTTCATCTTTGTCAAGGACACATGAATTCTTCATAAAATTTCAAAAACAAAAACAAGTTTCTAAAGTCCCGTTTTATAACCATTTAGTTTTTTAAATTTTGAACATTATACTTGTTTTCTCCCAATTTTTCTACCATGGTTATCATCTTTGTCAAGGATACATTTGAATTCCTTAGCCCAAATTCCAAAAACAAAAAAAGTTTTTGAAAATACTCTTTTTAGTTTTCAAAAATTGGCTTGGTTTTTTAAAACATGAAAAGAATGTATTAGAAAAACAAAGAAATTTGTAGGTGGAAGTAGTGTTTATAAGCTTAATTTTCAAAAACCAAATTGTTATCAAATGAGGTTTATTTTTTTTAGTTTTTAAAACCTGAGTTGGTTTTTGAAAACAGTGAATAATGTAGAAGAAAAGAAAAAAAAATGGGTGGAATGAGTGTTTACAAACTTAATTTTCAAAAACCAAAAACAAAATGGTTATCAAACGCAGTCTAGAAAGTTATTCCAAACAGACTCTTCCTCTCCCTCTTTTTCTTTTTCGTCTTAAGTTTTATATGAAGTTCCAAATTAAACTCGATTCATTGTGTTTTAATTTCAGGTACGTGAGGAAGTGAAGGGAAAGATTGAATCTCTTCTATCATCTTTAAGGTTACCACTTTAAAACGAACTAGTTTCCCTTGATGTTGTTCTTATTGAATAATAACACCAACGAAAAATTTTCATCTGTTTAGAAACAATCAACCTAATGATCATAAGACTGAAGAACATACAACCTTGAAGTTGAAGTTATGTTTTCTGAATCAAACTGCCTCCACCATTTTCACCAACAACGAGATCAAAGCGGAGAACGGCGACCCGTTACGAGTTGCATTATGTGATATGACCCACTTCAACGCCGTGGTCGTTTCCACGGGTCCTTTGTCGTCGGCTCAAATCGAATTTGTCGCCGTCGACGGAGAATTCAGCTCCGACTTGTGGCGAGAAGGAATAACTCGATCGAGTACTAATGATTTCAACAAAAGTGTTTTATCTGAAAGAGATGGGAAAAGACCATTGATGGTTGGCAATGATGTAAAAGTTTGTCTAAAAGATGGAGTTGGGTATATCAAGAGTTTGAGCTTCACAGACAACTCGAGTTGGATGAAATCTAAAAAGTTTAGACTCGGAGCAAGAATTATAGATGAGAAATTTTTAGCTCAGTTTCCAAGAATCGGAGAAGCTGTATCTCAACCATTTAGAGTCTTGGATCAACGAGGACAAGGTAGCTTCACTTTCAATAATATCTCTTTGTTTCTCGATTCTTATTTGGTAGACAATTCAGATTCTATTTTTGTGTTTTTGGATTCGCTCAATATGTGTTTGACAGATGTTTTTGAAAACTATTTTCGGATTGTGTGTGATCCAAAGGGTTGAAATTCTGAAAATAATTTTTTTTTAATGTATCTAGATTTTGAATTTGAAAATTTTAAATGTGGAATTAAATTTTAAAAAAAATGAAAATTTTAATAAATATAGTATTTCATGTTACAAACTCGATTCATTTTTGTGAAATTAATTAAAATTTGCATAATATAATGTATTATAAATTATATGATATTGTAAAATAATAATTATACAAAATTTATGACACCTGACATGTTTATAAATTAATTAACAATAGTTTATATTCAATTTAAATATTTAGTTGTCAAACACATTTAAACAATTGTTTAAATTGAGATCTAATTTCTGAATCTACTACCAAACACATATATCTGAAAACATGAAATACAACTTTGTTGTCATTGAATCTCTTTTTGTCCAAATTTCTATTTCAAATCACCAACAAAACGCACCCTAATTTCTAGGCCTCTCCTAAAGTTATGTTTTTTCTTACTTTTAAAATATTAGGGAACAAGAAGCACCACCCTCCAAAGGGGACAGATGAAGTATGGCGATTAGAAGGAATCGTCAAAAATGGTGTATATCATAATAACTTATCTTCTGAAAACATGGAAACGGTCGACGAATTCTTGAAGATTTATAATGGAAAGGGTCCTCTTTACTTGAGAAAGGTAACCAAGGAAAATGCTTAAAAGTTTTTCATTTGTGTTATATTGAATTAATTTTGGAGTTGTTGAATCTTGGCTTTGTATATGAATTGAAGCTACTTGGCAAGAAAGTCCCAAACAAGATATGGAAGGCAATGGTGGATAATGCTTTGGAGTGTGTCCCTATTTATTCCAACTTATCTCCAAGCTTTGAGGTTGGCTTTCTCCCTCCCTCAATCTAGAGGTGCAAGAAATTTATCCCTCACTCTTAACTTAATTTGTGTTGACATTTACATGAAAGATATGCCAATGGGAGCCTATGCTTAACTTTGAAGGTATATTATTACACTTTTGTTATTTTTTTCCATTTTAAAAAATAAAATAAAATTTATTACATTTTTTTTTATAGATTTACCATTGTTTCCACTTGGAGAAGCTTGAACTTCCAAGCCACAAACATGAAATGAGAAACAACTAAAAACTACCTAGCACTTGGATATTGAGTTGATGGTCAAATCGAAAGTGAGGCAGCCCAATGAAATCTAAATCTCTTATATATGTAAAGCGACAGACCACTACTCTCTTTTTTTTTTAGAACGAAAAGTAATTTCATTAATTGGTGTTGAATGAGAAATTTCGGCCAATCACAAATTGCCACGTCATTAATTTAAATTAATGATAAAATAAATTTGGGACCAATTACAAATTTACAGGTGTCCCTAAATTAAATTAATAGACATTAAAATTCGGCAAATCAAACAATGCCACTTGTCTATTATTTATACATAAGTCGAGTCCAATCAAATTATGCCACGTGGCAATTGGACTCGAGACTAATGTGACCCGAGATCATCGAAGAAATTGGGCTAAATTAAAATAATCCAATTTTATTAAAAATGAATTGGATTAAAGCCCAAAAGAGCCAAAGCCCAAAGATTGGGCCCAGGCCCAAGCCCATATGAGGTCCACCAAACTCTATAAATAGAGGAGGTCACCATTCATTTTGGGCATTCAGACAGAGTCAGAGAAGCCATAGCCCAGAGCATTCTCCCAAGGTCCAGAGTCTTCAGAAGTCAGAGAGTCCAAAGAATTCAGAAGATCCAAGATTCAGAATTCGGCCATCTCCAGACTCAGAAGATCCAACGACTGAAAGACTCCAAAGAATCAACCGTTTCTTCATCAAGATACAAGTTGAAGATTCAACCTTCCTCCGTCTGAGATCAAGCTTGTCGGCCCTCAGATCAGACTCCATATTTTGA

mRNA sequence

ATGGAGAGTGCTACAAAAAGAAACTTTCCAAATTTTCACAAGGTTGAAGAAGATGAAGATGAAGATGATCACCCTTCTCAAACTAACCCTAAAAGAATCTGCAACTCTACTGGGTTTGCTGTCTCTCTCTCTTTCTTTTTCTTCTTCTTCTTCTCTTCCCCTTTTTCAAGAAATGGGTTTCTTGATTTTGCCAGTTATGAAGCTGAATCTGACATTGGATCAGCTGAGCTTTTCTCTGTTGAATCTGAATCACACTCTCAAATAGAATCTTTCCTTCGTCGAATGGTACGTGAGGAAGTGAAGGGAAAGATTGAATCTCTTCTATCATCTTTAAGAAACAATCAACCTAATGATCATAAGACTGAAGAACATACAACCTTGAAGTTGAAGTTATGTTTTCTGAATCAAACTGCCTCCACCATTTTCACCAACAACGAGATCAAAGCGGAGAACGGCGACCCGTTACGAGTTGCATTATGTGATATGACCCACTTCAACGCCGTGGTCGTTTCCACGGGTCCTTTGTCGTCGGCTCAAATCGAATTTGTCGCCGTCGACGGAGAATTCAGCTCCGACTTGTGGCGAGAAGGAATAACTCGATCGAGTACTAATGATTTCAACAAAAGTGTTTTATCTGAAAGAGATGGGAAAAGACCATTGATGGTTGGCAATGATGTAAAAGTTTGTCTAAAAGATGGAGTTGGGTATATCAAGAGTTTGAGCTTCACAGACAACTCGAGTTGGATGAAATCTAAAAAGTTTAGACTCGGAGCAAGAATTATAGATGAGAAATTTTTAGCTCAGTTTCCAAGAATCGGAGAAGCTGTATCTCAACCATTTAGAGTCTTGGATCAACGAGGACAAGGGAACAAGAAGCACCACCCTCCAAAGGGGACAGATGAAGTATGGCGATTAGAAGGAATCGTCAAAAATGGTGTATATCATAATAACTTATCTTCTGAAAACATGGAAACGGTCGACGAATTCTTGAAGATTTATAATGGAAAGGGTCCTCTTTACTTGAGAAAGCTACTTGGCAAGAAAGTCCCAAACAAGATATGGAAGGCAATGGTGGATAATGCTTTGGAGTGTGTCCCTATTTATTCCAACTTATCTCCAAGCTTTGAGAGTCAGAGAAGCCATAGCCCAGAGCATTCTCCCAAGGTCCAGAGTCTTCAGAAGTCAGAGAGTCCAAAGAATTCAGAAGATCCAAGATTCAGAATTCGGCCATCTCCAGACTCAGAAGATCCAACGACTGAAAGACTCCAAAGAATCAACCGTTTCTTCATCAAGATACAAGTTGAAGATTCAACCTTCCTCCGTCTGAGATCAAGCTTGTCGGCCCTCAGATCAGACTCCATATTTTGA

Coding sequence (CDS)

ATGGAGAGTGCTACAAAAAGAAACTTTCCAAATTTTCACAAGGTTGAAGAAGATGAAGATGAAGATGATCACCCTTCTCAAACTAACCCTAAAAGAATCTGCAACTCTACTGGGTTTGCTGTCTCTCTCTCTTTCTTTTTCTTCTTCTTCTTCTCTTCCCCTTTTTCAAGAAATGGGTTTCTTGATTTTGCCAGTTATGAAGCTGAATCTGACATTGGATCAGCTGAGCTTTTCTCTGTTGAATCTGAATCACACTCTCAAATAGAATCTTTCCTTCGTCGAATGGTACGTGAGGAAGTGAAGGGAAAGATTGAATCTCTTCTATCATCTTTAAGAAACAATCAACCTAATGATCATAAGACTGAAGAACATACAACCTTGAAGTTGAAGTTATGTTTTCTGAATCAAACTGCCTCCACCATTTTCACCAACAACGAGATCAAAGCGGAGAACGGCGACCCGTTACGAGTTGCATTATGTGATATGACCCACTTCAACGCCGTGGTCGTTTCCACGGGTCCTTTGTCGTCGGCTCAAATCGAATTTGTCGCCGTCGACGGAGAATTCAGCTCCGACTTGTGGCGAGAAGGAATAACTCGATCGAGTACTAATGATTTCAACAAAAGTGTTTTATCTGAAAGAGATGGGAAAAGACCATTGATGGTTGGCAATGATGTAAAAGTTTGTCTAAAAGATGGAGTTGGGTATATCAAGAGTTTGAGCTTCACAGACAACTCGAGTTGGATGAAATCTAAAAAGTTTAGACTCGGAGCAAGAATTATAGATGAGAAATTTTTAGCTCAGTTTCCAAGAATCGGAGAAGCTGTATCTCAACCATTTAGAGTCTTGGATCAACGAGGACAAGGGAACAAGAAGCACCACCCTCCAAAGGGGACAGATGAAGTATGGCGATTAGAAGGAATCGTCAAAAATGGTGTATATCATAATAACTTATCTTCTGAAAACATGGAAACGGTCGACGAATTCTTGAAGATTTATAATGGAAAGGGTCCTCTTTACTTGAGAAAGCTACTTGGCAAGAAAGTCCCAAACAAGATATGGAAGGCAATGGTGGATAATGCTTTGGAGTGTGTCCCTATTTATTCCAACTTATCTCCAAGCTTTGAGAGTCAGAGAAGCCATAGCCCAGAGCATTCTCCCAAGGTCCAGAGTCTTCAGAAGTCAGAGAGTCCAAAGAATTCAGAAGATCCAAGATTCAGAATTCGGCCATCTCCAGACTCAGAAGATCCAACGACTGAAAGACTCCAAAGAATCAACCGTTTCTTCATCAAGATACAAGTTGAAGATTCAACCTTCCTCCGTCTGAGATCAAGCTTGTCGGCCCTCAGATCAGACTCCATATTTTGA

Protein sequence

MESATKRNFPNFHKVEEDEDEDDHPSQTNPKRICNSTGFAVSLSFFFFFFFSSPFSRNGFLDFASYEAESDIGSAELFSVESESHSQIESFLRRMVREEVKGKIESLLSSLRNNQPNDHKTEEHTTLKLKLCFLNQTASTIFTNNEIKAENGDPLRVALCDMTHFNAVVVSTGPLSSAQIEFVAVDGEFSSDLWREGITRSSTNDFNKSVLSERDGKRPLMVGNDVKVCLKDGVGYIKSLSFTDNSSWMKSKKFRLGARIIDEKFLAQFPRIGEAVSQPFRVLDQRGQGNKKHHPPKGTDEVWRLEGIVKNGVYHNNLSSENMETVDEFLKIYNGKGPLYLRKLLGKKVPNKIWKAMVDNALECVPIYSNLSPSFESQRSHSPEHSPKVQSLQKSESPKNSEDPRFRIRPSPDSEDPTTERLQRINRFFIKIQVEDSTFLRLRSSLSALRSDSIF
Homology
BLAST of Lag0036432 vs. NCBI nr
Match: XP_008451138.1 (PREDICTED: calmodulin-binding protein 60 B-like [Cucumis melo])

HSP 1 Score: 327.4 bits (838), Expect = 2.0e-85
Identity = 192/383 (50.13%), Postives = 245/383 (63.97%), Query Frame = 0

Query: 1   MESATKRNFPNFHKVEEDEDEDDHPSQTNPKRICNSTGFAVSLSFFFFFFFSSPFSRNGF 60
           ME   KR+F N H    D + D  PS+T+PKR  NS                +P S    
Sbjct: 1   MERDKKRDFQNLH----DNEHDYRPSETSPKRTSNS--------------IPTPGS---- 60

Query: 61  LDFASYEAESDIGSAELFSVESESHSQIESFLRRMVREEVKGKIESLL-----SSLRNNQ 120
            + AS +  ++I S +L+       SQ+E+ L R+V EE   K  S +     SS    Q
Sbjct: 61  -EVASDDIVANILSPQLW-------SQMEAILCRLVSEEFDRKFMSYVQFLPSSSSSTVQ 120

Query: 121 PNDHKTEEHTTLKLKLCFLNQTASTIFTNNEIKAENGDPLRVALCDMTHFNAVVVSTGPL 180
            N+H  EE  T   +LCFLNQ  STIFTNNEI+  NGD L+VAL D  + NA +VS GPL
Sbjct: 121 LNNHNNEE--TTNFRLCFLNQIPSTIFTNNEIETGNGDALQVALIDDNNSNA-IVSDGPL 180

Query: 181 SSAQIEFVAVDGEFSSDLWREGITRSSTNDFNKSVLSERDGKRPLMVGNDVKVCLKDGVG 240
           S AQIE   +DG+             S    ++++LS RDGKRPL+VG+D+K+ LK+GVG
Sbjct: 181 SIAQIEVSVLDGD-------------SDKFMSQNILSPRDGKRPLIVGDDLKLHLKNGVG 240

Query: 241 YIKSLSFTDNSSWMKSKKFRLGARIIDEKFLAQFPRIGEAVSQPFRVLDQRGQGNKKHHP 300
           +I+SLSFTDNSSW++SK+FRLG RI+D+K  ++FPRI E VSQPFRVLD RG+ N+KHHP
Sbjct: 241 FIQSLSFTDNSSWVRSKQFRLGLRIVDDKIQSKFPRIRETVSQPFRVLDHRGEVNQKHHP 300

Query: 301 PKGTDEVWRLEGIVKNGVYHNNLSSENMETVDEFLKIYNGKGPLYLRKLLGKKVPNKIWK 360
           P+  DE+WRLE I +NGVYH  LSS+ + TV +FL+ Y  KGPLYLRKLLGK V NK W 
Sbjct: 301 PQREDEIWRLERIGRNGVYHKLLSSQGINTVGDFLRTYEKKGPLYLRKLLGKNVSNKSWN 337

Query: 361 AMVDNALECVPIYSNLSPSFESQ 379
           AMV NALEC+P+  N +PSFE Q
Sbjct: 361 AMVTNALECIPLVKNFAPSFERQ 337

BLAST of Lag0036432 vs. NCBI nr
Match: XP_022961393.1 (calmodulin-binding protein 60 G-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 320.9 bits (821), Expect = 1.8e-83
Identity = 162/294 (55.10%), Postives = 218/294 (74.15%), Query Frame = 0

Query: 89  ESFLRRMVREEVKGKIESLLSSLRNNQPNDHKTEEHT-----TLKLKLCFLNQTASTIFT 148
           E F+R +V E V+ ++   + S+ ++Q + H  +E T       K KL FLNQT+ TIFT
Sbjct: 54  EGFIRSVVHEVVELQVNYYIHSILSSQLSIHIVKEETINGFGASKFKLLFLNQTSPTIFT 113

Query: 149 NNEIKAENGDPLRVALCDMTHFNAVVVSTGPLSSAQIEFVAVDGEFSSDLWREGITRSST 208
           NNEI+A NG PL VA+CD T+ N  ++ TGPLSSA +E V ++ EF SD  R   +  ++
Sbjct: 114 NNEIEARNGKPLLVAMCDTTNSNG-IIKTGPLSSALVELVVLNLEFGSD-QRVDESSWTS 173

Query: 209 NDFNKSVLSERDGKRPLMVGNDVKVCLKDGVGYIKSLSFTDNSSWMKSKKFRLGARIIDE 268
           +DFN +V+S+R+GKRPL+VGND+ +CLK+GVG+IK LSF+DNSSW KSKKFRLGA+++D+
Sbjct: 174 SDFNNNVVSKREGKRPLIVGNDMIICLKNGVGFIKGLSFSDNSSWTKSKKFRLGAKVVDK 233

Query: 269 KFLAQFPRIGEAVSQPFRVLDQRGQGNKKHHPPKGTDEVWRLEGIVKNGVYHNNLSSENM 328
             LA+FPRI EAVS+PFRV+D RG+  KKHHPP   DE+WRLEGI K+GVYHN LSSE +
Sbjct: 234 NILAKFPRIVEAVSKPFRVMDHRGEVYKKHHPPSREDEIWRLEGIGKDGVYHNRLSSEGI 293

Query: 329 ETVDEFLKIYNGKGPLYLRKLLGKKVPNKIWKAMVDNALECVPIYSNL--SPSF 376
           + VD+FL  Y+ KGP+Y+RKLLG +VP   WK MV+NALEC    +++  +PSF
Sbjct: 294 KNVDDFLNAYHKKGPIYVRKLLGGRVPQNTWKRMVNNALECDAFIASMASNPSF 345

BLAST of Lag0036432 vs. NCBI nr
Match: XP_022968714.1 (calmodulin-binding protein 60 G-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 318.2 bits (814), Expect = 1.2e-82
Identity = 161/294 (54.76%), Postives = 217/294 (73.81%), Query Frame = 0

Query: 89  ESFLRRMVREEVKGKIESLLSSLRNNQPNDHKTEEHT-----TLKLKLCFLNQTASTIFT 148
           E F+R +V E V+ ++   + S+ ++Q ++H  +E T       K KL FLNQTA TIFT
Sbjct: 54  EGFIRSVVHEVVELQVNYYIRSILSSQLSNHIGKEATINGFGASKFKLLFLNQTAPTIFT 113

Query: 149 NNEIKAENGDPLRVALCDMTHFNAVVVSTGPLSSAQIEFVAVDGEFSSDLWREGITRSST 208
           NNEI+A NG PL VA+CD T+ N  ++ TGPLSSA +E V ++ EF SD  R   +  ++
Sbjct: 114 NNEIEARNGKPLLVAICDTTNSNG-IIKTGPLSSALVELVVLNLEFGSD-QRVDESSWTS 173

Query: 209 NDFNKSVLSERDGKRPLMVGNDVKVCLKDGVGYIKSLSFTDNSSWMKSKKFRLGARIIDE 268
            DFN +V+S+R+GKRPL+VGND+ +CLK+GVG+IK LSF+DNSSW KSKKFRLGA+++D+
Sbjct: 174 RDFNNNVVSKREGKRPLIVGNDMIICLKNGVGFIKGLSFSDNSSWTKSKKFRLGAKVVDK 233

Query: 269 KFLAQFPRIGEAVSQPFRVLDQRGQGNKKHHPPKGTDEVWRLEGIVKNGVYHNNLSSENM 328
             LA+FPRI EAVS+PFRV+D RG+  KKHHPP+  DE+WRLEGI K+G+YHN L S+ +
Sbjct: 234 NILAKFPRIVEAVSKPFRVMDHRGKVYKKHHPPRREDEIWRLEGIGKDGIYHNRLFSKGI 293

Query: 329 ETVDEFLKIYNGKGPLYLRKLLGKKVPNKIWKAMVDNALECVPIYSNL--SPSF 376
           + VD+FLK Y+ KGP Y RKLLG +VP   WK MV+NALEC    +++  +PSF
Sbjct: 294 KNVDDFLKAYHKKGPTYFRKLLGGRVPQNTWKRMVNNALECDAFIASMASNPSF 345

BLAST of Lag0036432 vs. NCBI nr
Match: XP_022968713.1 (calmodulin-binding protein 60 G-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 317.8 bits (813), Expect = 1.5e-82
Identity = 163/296 (55.07%), Postives = 218/296 (73.65%), Query Frame = 0

Query: 89  ESFLRRMVREEVKGKIESLLSSL--RNNQPNDHKTEEHT-----TLKLKLCFLNQTASTI 148
           E F+R +V E V+ ++   + S+  RN+Q ++H  +E T       K KL FLNQTA TI
Sbjct: 54  EGFIRSVVHEVVELQVNYYIRSILSRNSQLSNHIGKEATINGFGASKFKLLFLNQTAPTI 113

Query: 149 FTNNEIKAENGDPLRVALCDMTHFNAVVVSTGPLSSAQIEFVAVDGEFSSDLWREGITRS 208
           FTNNEI+A NG PL VA+CD T+ N  ++ TGPLSSA +E V ++ EF SD  R   +  
Sbjct: 114 FTNNEIEARNGKPLLVAICDTTNSNG-IIKTGPLSSALVELVVLNLEFGSD-QRVDESSW 173

Query: 209 STNDFNKSVLSERDGKRPLMVGNDVKVCLKDGVGYIKSLSFTDNSSWMKSKKFRLGARII 268
           ++ DFN +V+S+R+GKRPL+VGND+ +CLK+GVG+IK LSF+DNSSW KSKKFRLGA+++
Sbjct: 174 TSRDFNNNVVSKREGKRPLIVGNDMIICLKNGVGFIKGLSFSDNSSWTKSKKFRLGAKVV 233

Query: 269 DEKFLAQFPRIGEAVSQPFRVLDQRGQGNKKHHPPKGTDEVWRLEGIVKNGVYHNNLSSE 328
           D+  LA+FPRI EAVS+PFRV+D RG+  KKHHPP+  DE+WRLEGI K+G+YHN L S+
Sbjct: 234 DKNILAKFPRIVEAVSKPFRVMDHRGKVYKKHHPPRREDEIWRLEGIGKDGIYHNRLFSK 293

Query: 329 NMETVDEFLKIYNGKGPLYLRKLLGKKVPNKIWKAMVDNALECVPIYSNL--SPSF 376
            ++ VD+FLK Y+ KGP Y RKLLG +VP   WK MV+NALEC    +++  +PSF
Sbjct: 294 GIKNVDDFLKAYHKKGPTYFRKLLGGRVPQNTWKRMVNNALECDAFIASMASNPSF 347

BLAST of Lag0036432 vs. NCBI nr
Match: XP_022961394.1 (calmodulin-binding protein 60 G-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 316.6 bits (810), Expect = 3.4e-82
Identity = 160/288 (55.56%), Postives = 214/288 (74.31%), Query Frame = 0

Query: 95  MVREEVKGKIESLLSSLRNNQPNDHKTEEHT-----TLKLKLCFLNQTASTIFTNNEIKA 154
           MV E V+ ++   + S+ ++Q + H  +E T       K KL FLNQT+ TIFTNNEI+A
Sbjct: 1   MVHEVVELQVNYYIHSILSSQLSIHIVKEETINGFGASKFKLLFLNQTSPTIFTNNEIEA 60

Query: 155 ENGDPLRVALCDMTHFNAVVVSTGPLSSAQIEFVAVDGEFSSDLWREGITRSSTNDFNKS 214
            NG PL VA+CD T+ N  ++ TGPLSSA +E V ++ EF SD  R   +  +++DFN +
Sbjct: 61  RNGKPLLVAMCDTTNSNG-IIKTGPLSSALVELVVLNLEFGSD-QRVDESSWTSSDFNNN 120

Query: 215 VLSERDGKRPLMVGNDVKVCLKDGVGYIKSLSFTDNSSWMKSKKFRLGARIIDEKFLAQF 274
           V+S+R+GKRPL+VGND+ +CLK+GVG+IK LSF+DNSSW KSKKFRLGA+++D+  LA+F
Sbjct: 121 VVSKREGKRPLIVGNDMIICLKNGVGFIKGLSFSDNSSWTKSKKFRLGAKVVDKNILAKF 180

Query: 275 PRIGEAVSQPFRVLDQRGQGNKKHHPPKGTDEVWRLEGIVKNGVYHNNLSSENMETVDEF 334
           PRI EAVS+PFRV+D RG+  KKHHPP   DE+WRLEGI K+GVYHN LSSE ++ VD+F
Sbjct: 181 PRIVEAVSKPFRVMDHRGEVYKKHHPPSREDEIWRLEGIGKDGVYHNRLSSEGIKNVDDF 240

Query: 335 LKIYNGKGPLYLRKLLGKKVPNKIWKAMVDNALECVPIYSNL--SPSF 376
           L  Y+ KGP+Y+RKLLG +VP   WK MV+NALEC    +++  +PSF
Sbjct: 241 LNAYHKKGPIYVRKLLGGRVPQNTWKRMVNNALECDAFIASMASNPSF 286

BLAST of Lag0036432 vs. ExPASy Swiss-Prot
Match: F4K2R6 (Calmodulin-binding protein 60 G OS=Arabidopsis thaliana OX=3702 GN=CBP60G PE=1 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 9.5e-51
Identity = 111/280 (39.64%), Postives = 172/280 (61.43%), Query Frame = 0

Query: 87  QIESFLRRMVREEVKGKIESLLSSLRNNQPNDHKTEEHTTLKLKLCFLNQTASTIFTNNE 146
           Q+E+ +RR+VREE++  ++  LSS   +          +  +LKLCF+N   S+IFT ++
Sbjct: 44  QMENMIRRIVREEIQRSLQPFLSSSCVSMERSRSETPSSRSRLKLCFINSPPSSIFTGSK 103

Query: 147 IKAENGDPLRVALCDMTHFNAVVVSTGPLSSAQIEFVAVDGEFSSDLWREGITRSSTNDF 206
           I+AE+G PL + L D T     +VSTGP SS+++E V ++ +F+ + W       +   F
Sbjct: 104 IEAEDGSPLVIELVDAT--TNTLVSTGPFSSSRVELVPLNADFTEESW-------TVEGF 163

Query: 207 NKSVLSERDGKRPLMVGNDVKVCLKDGVGYIK-SLSFTDNSSWMKSKKFRLGARIIDEKF 266
           N+++L++R+GKRPL+ G D+ V LK+GVG I   ++F+DNSSW +S+KFRLGA++  +  
Sbjct: 164 NRNILTQREGKRPLLTG-DLTVMLKNGVGVITGDIAFSDNSSWTRSRKFRLGAKLTGDGA 223

Query: 267 LAQFPRIGEAVSQPFRVLDQRGQGNKKHHPPKGTDEVWRLEGIVKNGVYHNNLSSENMET 326
           +       EA S+ F   DQRG+  KKHHPP  +DEVWRLE I K+GV    L+   + T
Sbjct: 224 V-------EARSEAFGCRDQRGESYKKHHPPCPSDEVWRLEKIAKDGVSATRLAERKILT 283

Query: 327 VDEFLKIYNGKGPLYLRKLLGKKVPNKIWKAMVDNALECV 366
           V +F ++Y       L  ++G  V  K W  +V +A++CV
Sbjct: 284 VKDFRRLYT-VNRNELHNIIGAGVSKKTWNTIVSHAMDCV 305

BLAST of Lag0036432 vs. ExPASy Swiss-Prot
Match: C0SVV6 (Calmodulin-binding protein 60 A OS=Arabidopsis thaliana OX=3702 GN=CBP60A PE=2 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.6e-45
Identity = 107/294 (36.39%), Postives = 175/294 (59.52%), Query Frame = 0

Query: 88  IESFLRRMVREEVKGKIESLLSSLRNNQPNDHKTEEHTTLKLKLCFLNQTASTIFTNNEI 147
           +E  +R++V+EEV+  +   L+ ++     +    E   L+LK  FLN  +  +FT+  I
Sbjct: 32  LEPLIRKVVKEEVELALGKHLAGIKWICEKETHPLESRNLQLK--FLNNLSLPVFTSARI 91

Query: 148 KAENGDPLRVALCDMTHFNAVVVSTGPLSSAQIEFVAVDGEFSSDLWREGITRSSTNDFN 207
           + + G  +RV L D +     + S+GP SSA++E   V+G+F+S      ++  +  D  
Sbjct: 92  EGDEGQAIRVGLIDPS--TGQIFSSGPASSAKLEVFVVEGDFNS------VSDWTDEDIR 151

Query: 208 KSVLSERDGKRPLMVGNDVKVCLKDGVGYIKSLSFTDNSSWMKSKKFRLGARIIDEKFLA 267
            +++ ER+GK+PL+ GN V   L DG+G +  +SFTDNSSW +S+KFRLG RI+D+    
Sbjct: 152 NNIVREREGKKPLLNGN-VFAVLNDGIGVMDEISFTDNSSWTRSRKFRLGVRIVDQ---F 211

Query: 268 QFPRIGEAVSQPFRVLDQRGQGNKKHHPPKGTDEVWRLEGIVKNGVYHNNLSSENMETVD 327
            + +I EA+++ F V D RG+  KKHHPP   DEVWRLE I K+G +H  L+  N+ TV 
Sbjct: 212 DYVKIREAITESFVVRDHRGELYKKHHPPSLFDEVWRLEKIGKDGAFHRRLNLSNINTVK 271

Query: 328 EFLKIYNGKGPLYLRKLLGKKVPNKIWKAMVDNALECV---PIYSNLSPSFESQ 379
           +FL  ++      LR++LG  + +K+W+  +D+A  CV    ++   +P F+ +
Sbjct: 272 DFLTHFH-LNSSKLRQVLGTGMSSKMWEITLDHARSCVLDSSVHVYQAPGFQKK 310

BLAST of Lag0036432 vs. ExPASy Swiss-Prot
Match: Q9FKL6 (Calmodulin-binding protein 60 B OS=Arabidopsis thaliana OX=3702 GN=CBP60B PE=2 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 2.5e-43
Identity = 108/284 (38.03%), Postives = 166/284 (58.45%), Query Frame = 0

Query: 86  SQIESFLRRMVREEVKGKIESL----LSSLRNNQPNDHKTEEHTTLKLKLCFLNQTASTI 145
           S +E  LRR+V EE++  +  L    L+    + P   + E     KL+L F ++ +  +
Sbjct: 49  SSLEPILRRVVSEELERALAKLGPARLTGSSGSSPK--RIEGPDGRKLQLHFKSRLSLPL 108

Query: 146 FTNNEIKAENGDPLRVALCDMTHFNAVVVSTGPLSSAQIEFVAVDGEFSSDLWREGITRS 205
           FT  +++ E G  + V L D     AVV   GP +SA++  V ++G+F++    E     
Sbjct: 109 FTGGKVEGEQGAVIHVVLIDANTGRAVVY--GPEASAKLHIVVLEGDFNT----EDDEDW 168

Query: 206 STNDFNKSVLSERDGKRPLMVGNDVKVCLKDGVGYIKSLSFTDNSSWMKSKKFRLGARII 265
           +  +F   V+ ER GKRPL+ G +V V LK+GVG +  L FTDNSSW++S+KFRLG R++
Sbjct: 169 TQEEFESHVVKERSGKRPLLTG-EVYVTLKEGVGTLGELVFTDNSSWIRSRKFRLGLRVV 228

Query: 266 DEKFLAQFPRIGEAVSQPFRVLDQRGQGNKKHHPPKGTDEVWRLEGIVKNGVYHNNLSSE 325
                    RI EA ++ F V D RG+  KKH+PP   D+VWRL+ I K+G +H  L++E
Sbjct: 229 SG--CCDGMRIREAKTEAFVVKDHRGELYKKHYPPALNDDVWRLDKIGKDGAFHKKLTAE 288

Query: 326 NMETVDEFLKIYNGKGPLYLRKLLGKKVPNKIWKAMVDNALECV 366
            + TV++FL++     P  LR +LG  + NK+W A+V++A  CV
Sbjct: 289 GINTVEDFLRVMVKDSP-KLRTILGSGMSNKMWDALVEHAKTCV 320

BLAST of Lag0036432 vs. ExPASy Swiss-Prot
Match: C0SV51 (Calmodulin-binding protein 60 C OS=Arabidopsis thaliana OX=3702 GN=CBP60C PE=2 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 1.2e-42
Identity = 105/283 (37.10%), Postives = 164/283 (57.95%), Query Frame = 0

Query: 86  SQIESFLRRMVREEVKGKIESLLSSLRNNQPNDHKTEEHTTLKLKLCFLNQTASTIFTNN 145
           S +E  LRR+V EEV+  +  L  +  + + +  + E      L+L F ++ +  +FT  
Sbjct: 55  SSLEPILRRVVSEEVERALAKLGPARLSERSSPKRIEGIGGRNLQLQFRSRLSVPLFTGG 114

Query: 146 EIKAENGDPLRVALCDMTHFNAVVVSTGPLSSAQIEFVAVDGEFSS---DLWREGITRSS 205
           +I+ E G  + V L DMT     V++ GP +SA+++ V +DG+F++   D W       S
Sbjct: 115 KIEGEQGAAIHVVLLDMT--TGHVLTVGPEASAKLDVVVLDGDFNTEDDDGW-------S 174

Query: 206 TNDFNKSVLSERDGKRPLMVGNDVKVCLKDGVGYIKSLSFTDNSSWMKSKKFRLGARIID 265
             +F   ++ ER GKRPL+ G DV+V LK+GVG +  L FTDNSSW++ +KFRLG R+  
Sbjct: 175 GEEFEGHLVKERQGKRPLLTG-DVQVTLKEGVGTLGELIFTDNSSWIRCRKFRLGLRV-- 234

Query: 266 EKFLAQFPRIGEAVSQPFRVLDQRGQGNKKHHPPKGTDEVWRLEGIVKNGVYHNNLSSEN 325
                +  R+ EA ++ F V D RG+  KKH+PP   DEVWRLE I K+G +H  L+   
Sbjct: 235 SSGYCEGMRVREAKTEAFTVKDHRGELYKKHYPPALDDEVWRLEKIGKDGAFHKKLNKAG 294

Query: 326 METVDEFLKIYNGKGPLYLRKLLGKKVPNKIWKAMVDNALECV 366
           +  V EFL++   K    LR +LG  + N++W+ + +++  CV
Sbjct: 295 IYNVKEFLRLM-VKDSQKLRTILGSGMSNRMWETLAEHSKTCV 324

BLAST of Lag0036432 vs. ExPASy Swiss-Prot
Match: F4IPM3 (Calmodulin-binding protein 60 E OS=Arabidopsis thaliana OX=3702 GN=CBP60E PE=2 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 1.6e-42
Identity = 107/284 (37.68%), Postives = 162/284 (57.04%), Query Frame = 0

Query: 86  SQIESFLRRMVREEVKGKIESL-LSSLRNNQPNDHKTEEHTTLKLKLCFLNQTASTIFTN 145
           S +E   RR+V EEV+  +  L  + L +  P   + ++     L+L F  +    +FT 
Sbjct: 45  SSLEPLFRRIVSEEVERALSRLGNAKLTSRSPEPKRIQDRNGRNLQLHFRTRMPPHLFTG 104

Query: 146 NEIKAENGDPLRVALCDMTHFNAVVVSTGPLSSAQIEFVAVDGEFSSDLWREGITRSSTN 205
            +++ E G  + V L D    N  VV TG  S++++  V ++G+F +D   E  TR    
Sbjct: 105 GKVEGERGSAIHVVLIDANTGN--VVQTGEESASKLNVVVLEGDF-NDEDDEDWTREHFE 164

Query: 206 DFNKSVLSERDGKRPLMVGNDVKVCLKDGVGYIKSLSFTDNSSWMKSKKFRLGARIID-- 265
            F    + ER+GKRP++ G D ++ LK+GVG +  L+FTDNSSW++S+KFRLG +     
Sbjct: 165 SFE---VKEREGKRPILTG-DTQIVLKEGVGTLGELTFTDNSSWIRSRKFRLGVKPASGY 224

Query: 266 -EKFLAQFPRIGEAVSQPFRVLDQRGQGNKKHHPPKGTDEVWRLEGIVKNGVYHNNLSSE 325
            + F      I EA ++PF V D RG+  KKH+PP   DEVWRL+ I K+GV H  L   
Sbjct: 225 GDSFC-----IREAKTEPFAVKDHRGELYKKHYPPAVHDEVWRLDRIAKDGVLHKKLLKA 284

Query: 326 NMETVDEFLKIYNGKGPLYLRKLLGKKVPNKIWKAMVDNALECV 366
           N+ TV++FL++   K P  LR LLG  + N++W+  V++A  CV
Sbjct: 285 NIVTVEDFLRLL-VKDPQKLRNLLGSGMSNRMWENTVEHAKTCV 315

BLAST of Lag0036432 vs. ExPASy TrEMBL
Match: A0A1S3BQ83 (calmodulin-binding protein 60 B-like OS=Cucumis melo OX=3656 GN=LOC103492515 PE=3 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 9.5e-86
Identity = 192/383 (50.13%), Postives = 245/383 (63.97%), Query Frame = 0

Query: 1   MESATKRNFPNFHKVEEDEDEDDHPSQTNPKRICNSTGFAVSLSFFFFFFFSSPFSRNGF 60
           ME   KR+F N H    D + D  PS+T+PKR  NS                +P S    
Sbjct: 1   MERDKKRDFQNLH----DNEHDYRPSETSPKRTSNS--------------IPTPGS---- 60

Query: 61  LDFASYEAESDIGSAELFSVESESHSQIESFLRRMVREEVKGKIESLL-----SSLRNNQ 120
            + AS +  ++I S +L+       SQ+E+ L R+V EE   K  S +     SS    Q
Sbjct: 61  -EVASDDIVANILSPQLW-------SQMEAILCRLVSEEFDRKFMSYVQFLPSSSSSTVQ 120

Query: 121 PNDHKTEEHTTLKLKLCFLNQTASTIFTNNEIKAENGDPLRVALCDMTHFNAVVVSTGPL 180
            N+H  EE  T   +LCFLNQ  STIFTNNEI+  NGD L+VAL D  + NA +VS GPL
Sbjct: 121 LNNHNNEE--TTNFRLCFLNQIPSTIFTNNEIETGNGDALQVALIDDNNSNA-IVSDGPL 180

Query: 181 SSAQIEFVAVDGEFSSDLWREGITRSSTNDFNKSVLSERDGKRPLMVGNDVKVCLKDGVG 240
           S AQIE   +DG+             S    ++++LS RDGKRPL+VG+D+K+ LK+GVG
Sbjct: 181 SIAQIEVSVLDGD-------------SDKFMSQNILSPRDGKRPLIVGDDLKLHLKNGVG 240

Query: 241 YIKSLSFTDNSSWMKSKKFRLGARIIDEKFLAQFPRIGEAVSQPFRVLDQRGQGNKKHHP 300
           +I+SLSFTDNSSW++SK+FRLG RI+D+K  ++FPRI E VSQPFRVLD RG+ N+KHHP
Sbjct: 241 FIQSLSFTDNSSWVRSKQFRLGLRIVDDKIQSKFPRIRETVSQPFRVLDHRGEVNQKHHP 300

Query: 301 PKGTDEVWRLEGIVKNGVYHNNLSSENMETVDEFLKIYNGKGPLYLRKLLGKKVPNKIWK 360
           P+  DE+WRLE I +NGVYH  LSS+ + TV +FL+ Y  KGPLYLRKLLGK V NK W 
Sbjct: 301 PQREDEIWRLERIGRNGVYHKLLSSQGINTVGDFLRTYEKKGPLYLRKLLGKNVSNKSWN 337

Query: 361 AMVDNALECVPIYSNLSPSFESQ 379
           AMV NALEC+P+  N +PSFE Q
Sbjct: 361 AMVTNALECIPLVKNFAPSFERQ 337

BLAST of Lag0036432 vs. ExPASy TrEMBL
Match: A0A6J1HBN4 (calmodulin-binding protein 60 G-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461979 PE=3 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 8.9e-84
Identity = 162/294 (55.10%), Postives = 218/294 (74.15%), Query Frame = 0

Query: 89  ESFLRRMVREEVKGKIESLLSSLRNNQPNDHKTEEHT-----TLKLKLCFLNQTASTIFT 148
           E F+R +V E V+ ++   + S+ ++Q + H  +E T       K KL FLNQT+ TIFT
Sbjct: 54  EGFIRSVVHEVVELQVNYYIHSILSSQLSIHIVKEETINGFGASKFKLLFLNQTSPTIFT 113

Query: 149 NNEIKAENGDPLRVALCDMTHFNAVVVSTGPLSSAQIEFVAVDGEFSSDLWREGITRSST 208
           NNEI+A NG PL VA+CD T+ N  ++ TGPLSSA +E V ++ EF SD  R   +  ++
Sbjct: 114 NNEIEARNGKPLLVAMCDTTNSNG-IIKTGPLSSALVELVVLNLEFGSD-QRVDESSWTS 173

Query: 209 NDFNKSVLSERDGKRPLMVGNDVKVCLKDGVGYIKSLSFTDNSSWMKSKKFRLGARIIDE 268
           +DFN +V+S+R+GKRPL+VGND+ +CLK+GVG+IK LSF+DNSSW KSKKFRLGA+++D+
Sbjct: 174 SDFNNNVVSKREGKRPLIVGNDMIICLKNGVGFIKGLSFSDNSSWTKSKKFRLGAKVVDK 233

Query: 269 KFLAQFPRIGEAVSQPFRVLDQRGQGNKKHHPPKGTDEVWRLEGIVKNGVYHNNLSSENM 328
             LA+FPRI EAVS+PFRV+D RG+  KKHHPP   DE+WRLEGI K+GVYHN LSSE +
Sbjct: 234 NILAKFPRIVEAVSKPFRVMDHRGEVYKKHHPPSREDEIWRLEGIGKDGVYHNRLSSEGI 293

Query: 329 ETVDEFLKIYNGKGPLYLRKLLGKKVPNKIWKAMVDNALECVPIYSNL--SPSF 376
           + VD+FL  Y+ KGP+Y+RKLLG +VP   WK MV+NALEC    +++  +PSF
Sbjct: 294 KNVDDFLNAYHKKGPIYVRKLLGGRVPQNTWKRMVNNALECDAFIASMASNPSF 345

BLAST of Lag0036432 vs. ExPASy TrEMBL
Match: A0A6J1I0G5 (calmodulin-binding protein 60 G-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467868 PE=3 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 5.7e-83
Identity = 161/294 (54.76%), Postives = 217/294 (73.81%), Query Frame = 0

Query: 89  ESFLRRMVREEVKGKIESLLSSLRNNQPNDHKTEEHT-----TLKLKLCFLNQTASTIFT 148
           E F+R +V E V+ ++   + S+ ++Q ++H  +E T       K KL FLNQTA TIFT
Sbjct: 54  EGFIRSVVHEVVELQVNYYIRSILSSQLSNHIGKEATINGFGASKFKLLFLNQTAPTIFT 113

Query: 149 NNEIKAENGDPLRVALCDMTHFNAVVVSTGPLSSAQIEFVAVDGEFSSDLWREGITRSST 208
           NNEI+A NG PL VA+CD T+ N  ++ TGPLSSA +E V ++ EF SD  R   +  ++
Sbjct: 114 NNEIEARNGKPLLVAICDTTNSNG-IIKTGPLSSALVELVVLNLEFGSD-QRVDESSWTS 173

Query: 209 NDFNKSVLSERDGKRPLMVGNDVKVCLKDGVGYIKSLSFTDNSSWMKSKKFRLGARIIDE 268
            DFN +V+S+R+GKRPL+VGND+ +CLK+GVG+IK LSF+DNSSW KSKKFRLGA+++D+
Sbjct: 174 RDFNNNVVSKREGKRPLIVGNDMIICLKNGVGFIKGLSFSDNSSWTKSKKFRLGAKVVDK 233

Query: 269 KFLAQFPRIGEAVSQPFRVLDQRGQGNKKHHPPKGTDEVWRLEGIVKNGVYHNNLSSENM 328
             LA+FPRI EAVS+PFRV+D RG+  KKHHPP+  DE+WRLEGI K+G+YHN L S+ +
Sbjct: 234 NILAKFPRIVEAVSKPFRVMDHRGKVYKKHHPPRREDEIWRLEGIGKDGIYHNRLFSKGI 293

Query: 329 ETVDEFLKIYNGKGPLYLRKLLGKKVPNKIWKAMVDNALECVPIYSNL--SPSF 376
           + VD+FLK Y+ KGP Y RKLLG +VP   WK MV+NALEC    +++  +PSF
Sbjct: 294 KNVDDFLKAYHKKGPTYFRKLLGGRVPQNTWKRMVNNALECDAFIASMASNPSF 345

BLAST of Lag0036432 vs. ExPASy TrEMBL
Match: A0A6J1HYX2 (calmodulin-binding protein 60 G-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467868 PE=3 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 7.5e-83
Identity = 163/296 (55.07%), Postives = 218/296 (73.65%), Query Frame = 0

Query: 89  ESFLRRMVREEVKGKIESLLSSL--RNNQPNDHKTEEHT-----TLKLKLCFLNQTASTI 148
           E F+R +V E V+ ++   + S+  RN+Q ++H  +E T       K KL FLNQTA TI
Sbjct: 54  EGFIRSVVHEVVELQVNYYIRSILSRNSQLSNHIGKEATINGFGASKFKLLFLNQTAPTI 113

Query: 149 FTNNEIKAENGDPLRVALCDMTHFNAVVVSTGPLSSAQIEFVAVDGEFSSDLWREGITRS 208
           FTNNEI+A NG PL VA+CD T+ N  ++ TGPLSSA +E V ++ EF SD  R   +  
Sbjct: 114 FTNNEIEARNGKPLLVAICDTTNSNG-IIKTGPLSSALVELVVLNLEFGSD-QRVDESSW 173

Query: 209 STNDFNKSVLSERDGKRPLMVGNDVKVCLKDGVGYIKSLSFTDNSSWMKSKKFRLGARII 268
           ++ DFN +V+S+R+GKRPL+VGND+ +CLK+GVG+IK LSF+DNSSW KSKKFRLGA+++
Sbjct: 174 TSRDFNNNVVSKREGKRPLIVGNDMIICLKNGVGFIKGLSFSDNSSWTKSKKFRLGAKVV 233

Query: 269 DEKFLAQFPRIGEAVSQPFRVLDQRGQGNKKHHPPKGTDEVWRLEGIVKNGVYHNNLSSE 328
           D+  LA+FPRI EAVS+PFRV+D RG+  KKHHPP+  DE+WRLEGI K+G+YHN L S+
Sbjct: 234 DKNILAKFPRIVEAVSKPFRVMDHRGKVYKKHHPPRREDEIWRLEGIGKDGIYHNRLFSK 293

Query: 329 NMETVDEFLKIYNGKGPLYLRKLLGKKVPNKIWKAMVDNALECVPIYSNL--SPSF 376
            ++ VD+FLK Y+ KGP Y RKLLG +VP   WK MV+NALEC    +++  +PSF
Sbjct: 294 GIKNVDDFLKAYHKKGPTYFRKLLGGRVPQNTWKRMVNNALECDAFIASMASNPSF 347

BLAST of Lag0036432 vs. ExPASy TrEMBL
Match: A0A6J1HDW8 (calmodulin-binding protein 60 G-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111461979 PE=3 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 1.7e-82
Identity = 160/288 (55.56%), Postives = 214/288 (74.31%), Query Frame = 0

Query: 95  MVREEVKGKIESLLSSLRNNQPNDHKTEEHT-----TLKLKLCFLNQTASTIFTNNEIKA 154
           MV E V+ ++   + S+ ++Q + H  +E T       K KL FLNQT+ TIFTNNEI+A
Sbjct: 1   MVHEVVELQVNYYIHSILSSQLSIHIVKEETINGFGASKFKLLFLNQTSPTIFTNNEIEA 60

Query: 155 ENGDPLRVALCDMTHFNAVVVSTGPLSSAQIEFVAVDGEFSSDLWREGITRSSTNDFNKS 214
            NG PL VA+CD T+ N  ++ TGPLSSA +E V ++ EF SD  R   +  +++DFN +
Sbjct: 61  RNGKPLLVAMCDTTNSNG-IIKTGPLSSALVELVVLNLEFGSD-QRVDESSWTSSDFNNN 120

Query: 215 VLSERDGKRPLMVGNDVKVCLKDGVGYIKSLSFTDNSSWMKSKKFRLGARIIDEKFLAQF 274
           V+S+R+GKRPL+VGND+ +CLK+GVG+IK LSF+DNSSW KSKKFRLGA+++D+  LA+F
Sbjct: 121 VVSKREGKRPLIVGNDMIICLKNGVGFIKGLSFSDNSSWTKSKKFRLGAKVVDKNILAKF 180

Query: 275 PRIGEAVSQPFRVLDQRGQGNKKHHPPKGTDEVWRLEGIVKNGVYHNNLSSENMETVDEF 334
           PRI EAVS+PFRV+D RG+  KKHHPP   DE+WRLEGI K+GVYHN LSSE ++ VD+F
Sbjct: 181 PRIVEAVSKPFRVMDHRGEVYKKHHPPSREDEIWRLEGIGKDGVYHNRLSSEGIKNVDDF 240

Query: 335 LKIYNGKGPLYLRKLLGKKVPNKIWKAMVDNALECVPIYSNL--SPSF 376
           L  Y+ KGP+Y+RKLLG +VP   WK MV+NALEC    +++  +PSF
Sbjct: 241 LNAYHKKGPIYVRKLLGGRVPQNTWKRMVNNALECDAFIASMASNPSF 286

BLAST of Lag0036432 vs. TAIR 10
Match: AT5G26920.1 (Cam-binding protein 60-like G )

HSP 1 Score: 202.6 bits (514), Expect = 6.8e-52
Identity = 111/280 (39.64%), Postives = 172/280 (61.43%), Query Frame = 0

Query: 87  QIESFLRRMVREEVKGKIESLLSSLRNNQPNDHKTEEHTTLKLKLCFLNQTASTIFTNNE 146
           Q+E+ +RR+VREE++  ++  LSS   +          +  +LKLCF+N   S+IFT ++
Sbjct: 44  QMENMIRRIVREEIQRSLQPFLSSSCVSMERSRSETPSSRSRLKLCFINSPPSSIFTGSK 103

Query: 147 IKAENGDPLRVALCDMTHFNAVVVSTGPLSSAQIEFVAVDGEFSSDLWREGITRSSTNDF 206
           I+AE+G PL + L D T     +VSTGP SS+++E V ++ +F+ + W       +   F
Sbjct: 104 IEAEDGSPLVIELVDAT--TNTLVSTGPFSSSRVELVPLNADFTEESW-------TVEGF 163

Query: 207 NKSVLSERDGKRPLMVGNDVKVCLKDGVGYIK-SLSFTDNSSWMKSKKFRLGARIIDEKF 266
           N+++L++R+GKRPL+ G D+ V LK+GVG I   ++F+DNSSW +S+KFRLGA++  +  
Sbjct: 164 NRNILTQREGKRPLLTG-DLTVMLKNGVGVITGDIAFSDNSSWTRSRKFRLGAKLTGDGA 223

Query: 267 LAQFPRIGEAVSQPFRVLDQRGQGNKKHHPPKGTDEVWRLEGIVKNGVYHNNLSSENMET 326
           +       EA S+ F   DQRG+  KKHHPP  +DEVWRLE I K+GV    L+   + T
Sbjct: 224 V-------EARSEAFGCRDQRGESYKKHHPPCPSDEVWRLEKIAKDGVSATRLAERKILT 283

Query: 327 VDEFLKIYNGKGPLYLRKLLGKKVPNKIWKAMVDNALECV 366
           V +F ++Y       L  ++G  V  K W  +V +A++CV
Sbjct: 284 VKDFRRLYT-VNRNELHNIIGAGVSKKTWNTIVSHAMDCV 305

BLAST of Lag0036432 vs. TAIR 10
Match: AT5G26920.2 (Cam-binding protein 60-like G )

HSP 1 Score: 201.4 bits (511), Expect = 1.5e-51
Identity = 110/280 (39.29%), Postives = 171/280 (61.07%), Query Frame = 0

Query: 87  QIESFLRRMVREEVKGKIESLLSSLRNNQPNDHKTEEHTTLKLKLCFLNQTASTIFTNNE 146
           Q+E+ +RR+VREE++  ++  LSS   +          +  +LKLCF+N   S+IFT ++
Sbjct: 44  QMENMIRRIVREEIQRSLQPFLSSSCVSMERSRSETPSSRSRLKLCFINSPPSSIFTGSK 103

Query: 147 IKAENGDPLRVALCDMTHFNAVVVSTGPLSSAQIEFVAVDGEFSSDLWREGITRSSTNDF 206
           I+AE+G PL + L D T     +VSTGP SS+++E V ++ +F+ + W       +   F
Sbjct: 104 IEAEDGSPLVIELVDAT--TNTLVSTGPFSSSRVELVPLNADFTEESW-------TVEGF 163

Query: 207 NKSVLSERDGKRPLMVGNDVKVCLKDGVGYIK-SLSFTDNSSWMKSKKFRLGARIIDEKF 266
           N+++L++R+GKRPL+ G D+ V LK+GVG I   ++F+DNSSW +S+KFRLGA++  +  
Sbjct: 164 NRNILTQREGKRPLLTG-DLTVMLKNGVGVITGDIAFSDNSSWTRSRKFRLGAKLTGDGA 223

Query: 267 LAQFPRIGEAVSQPFRVLDQRGQGNKKHHPPKGTDEVWRLEGIVKNGVYHNNLSSENMET 326
           +       EA S+ F   DQRG+  KKHHPP  +DEVWRLE I K+GV    L+   + T
Sbjct: 224 V-------EARSEAFGCRDQRGESYKKHHPPCPSDEVWRLEKIAKDGVSATRLAERKILT 283

Query: 327 VDEFLKIYNGKGPLYLRKLLGKKVPNKIWKAMVDNALECV 366
           V +F ++Y          ++G  V  K W  +V +A++CV
Sbjct: 284 VKDFRRLYT---------IIGAGVSKKTWNTIVSHAMDCV 297

BLAST of Lag0036432 vs. TAIR 10
Match: AT5G62570.1 (Calmodulin binding protein-like )

HSP 1 Score: 185.3 bits (469), Expect = 1.1e-46
Identity = 107/294 (36.39%), Postives = 175/294 (59.52%), Query Frame = 0

Query: 88  IESFLRRMVREEVKGKIESLLSSLRNNQPNDHKTEEHTTLKLKLCFLNQTASTIFTNNEI 147
           +E  +R++V+EEV+  +   L+ ++     +    E   L+LK  FLN  +  +FT+  I
Sbjct: 14  LEPLIRKVVKEEVELALGKHLAGIKWICEKETHPLESRNLQLK--FLNNLSLPVFTSARI 73

Query: 148 KAENGDPLRVALCDMTHFNAVVVSTGPLSSAQIEFVAVDGEFSSDLWREGITRSSTNDFN 207
           + + G  +RV L D +     + S+GP SSA++E   V+G+F+S      ++  +  D  
Sbjct: 74  EGDEGQAIRVGLIDPS--TGQIFSSGPASSAKLEVFVVEGDFNS------VSDWTDEDIR 133

Query: 208 KSVLSERDGKRPLMVGNDVKVCLKDGVGYIKSLSFTDNSSWMKSKKFRLGARIIDEKFLA 267
            +++ ER+GK+PL+ GN V   L DG+G +  +SFTDNSSW +S+KFRLG RI+D+    
Sbjct: 134 NNIVREREGKKPLLNGN-VFAVLNDGIGVMDEISFTDNSSWTRSRKFRLGVRIVDQ---F 193

Query: 268 QFPRIGEAVSQPFRVLDQRGQGNKKHHPPKGTDEVWRLEGIVKNGVYHNNLSSENMETVD 327
            + +I EA+++ F V D RG+  KKHHPP   DEVWRLE I K+G +H  L+  N+ TV 
Sbjct: 194 DYVKIREAITESFVVRDHRGELYKKHHPPSLFDEVWRLEKIGKDGAFHRRLNLSNINTVK 253

Query: 328 EFLKIYNGKGPLYLRKLLGKKVPNKIWKAMVDNALECV---PIYSNLSPSFESQ 379
           +FL  ++      LR++LG  + +K+W+  +D+A  CV    ++   +P F+ +
Sbjct: 254 DFLTHFH-LNSSKLRQVLGTGMSSKMWEITLDHARSCVLDSSVHVYQAPGFQKK 292

BLAST of Lag0036432 vs. TAIR 10
Match: AT5G62570.2 (Calmodulin binding protein-like )

HSP 1 Score: 185.3 bits (469), Expect = 1.1e-46
Identity = 107/294 (36.39%), Postives = 175/294 (59.52%), Query Frame = 0

Query: 88  IESFLRRMVREEVKGKIESLLSSLRNNQPNDHKTEEHTTLKLKLCFLNQTASTIFTNNEI 147
           +E  +R++V+EEV+  +   L+ ++     +    E   L+LK  FLN  +  +FT+  I
Sbjct: 32  LEPLIRKVVKEEVELALGKHLAGIKWICEKETHPLESRNLQLK--FLNNLSLPVFTSARI 91

Query: 148 KAENGDPLRVALCDMTHFNAVVVSTGPLSSAQIEFVAVDGEFSSDLWREGITRSSTNDFN 207
           + + G  +RV L D +     + S+GP SSA++E   V+G+F+S      ++  +  D  
Sbjct: 92  EGDEGQAIRVGLIDPS--TGQIFSSGPASSAKLEVFVVEGDFNS------VSDWTDEDIR 151

Query: 208 KSVLSERDGKRPLMVGNDVKVCLKDGVGYIKSLSFTDNSSWMKSKKFRLGARIIDEKFLA 267
            +++ ER+GK+PL+ GN V   L DG+G +  +SFTDNSSW +S+KFRLG RI+D+    
Sbjct: 152 NNIVREREGKKPLLNGN-VFAVLNDGIGVMDEISFTDNSSWTRSRKFRLGVRIVDQ---F 211

Query: 268 QFPRIGEAVSQPFRVLDQRGQGNKKHHPPKGTDEVWRLEGIVKNGVYHNNLSSENMETVD 327
            + +I EA+++ F V D RG+  KKHHPP   DEVWRLE I K+G +H  L+  N+ TV 
Sbjct: 212 DYVKIREAITESFVVRDHRGELYKKHHPPSLFDEVWRLEKIGKDGAFHRRLNLSNINTVK 271

Query: 328 EFLKIYNGKGPLYLRKLLGKKVPNKIWKAMVDNALECV---PIYSNLSPSFESQ 379
           +FL  ++      LR++LG  + +K+W+  +D+A  CV    ++   +P F+ +
Sbjct: 272 DFLTHFH-LNSSKLRQVLGTGMSSKMWEITLDHARSCVLDSSVHVYQAPGFQKK 310

BLAST of Lag0036432 vs. TAIR 10
Match: AT5G57580.1 (Calmodulin-binding protein )

HSP 1 Score: 177.9 bits (450), Expect = 1.8e-44
Identity = 108/284 (38.03%), Postives = 166/284 (58.45%), Query Frame = 0

Query: 86  SQIESFLRRMVREEVKGKIESL----LSSLRNNQPNDHKTEEHTTLKLKLCFLNQTASTI 145
           S +E  LRR+V EE++  +  L    L+    + P   + E     KL+L F ++ +  +
Sbjct: 49  SSLEPILRRVVSEELERALAKLGPARLTGSSGSSPK--RIEGPDGRKLQLHFKSRLSLPL 108

Query: 146 FTNNEIKAENGDPLRVALCDMTHFNAVVVSTGPLSSAQIEFVAVDGEFSSDLWREGITRS 205
           FT  +++ E G  + V L D     AVV   GP +SA++  V ++G+F++    E     
Sbjct: 109 FTGGKVEGEQGAVIHVVLIDANTGRAVVY--GPEASAKLHIVVLEGDFNT----EDDEDW 168

Query: 206 STNDFNKSVLSERDGKRPLMVGNDVKVCLKDGVGYIKSLSFTDNSSWMKSKKFRLGARII 265
           +  +F   V+ ER GKRPL+ G +V V LK+GVG +  L FTDNSSW++S+KFRLG R++
Sbjct: 169 TQEEFESHVVKERSGKRPLLTG-EVYVTLKEGVGTLGELVFTDNSSWIRSRKFRLGLRVV 228

Query: 266 DEKFLAQFPRIGEAVSQPFRVLDQRGQGNKKHHPPKGTDEVWRLEGIVKNGVYHNNLSSE 325
                    RI EA ++ F V D RG+  KKH+PP   D+VWRL+ I K+G +H  L++E
Sbjct: 229 SG--CCDGMRIREAKTEAFVVKDHRGELYKKHYPPALNDDVWRLDKIGKDGAFHKKLTAE 288

Query: 326 NMETVDEFLKIYNGKGPLYLRKLLGKKVPNKIWKAMVDNALECV 366
            + TV++FL++     P  LR +LG  + NK+W A+V++A  CV
Sbjct: 289 GINTVEDFLRVMVKDSP-KLRTILGSGMSNKMWDALVEHAKTCV 320

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008451138.12.0e-8550.13PREDICTED: calmodulin-binding protein 60 B-like [Cucumis melo][more]
XP_022961393.11.8e-8355.10calmodulin-binding protein 60 G-like isoform X1 [Cucurbita moschata][more]
XP_022968714.11.2e-8254.76calmodulin-binding protein 60 G-like isoform X2 [Cucurbita maxima][more]
XP_022968713.11.5e-8255.07calmodulin-binding protein 60 G-like isoform X1 [Cucurbita maxima][more]
XP_022961394.13.4e-8255.56calmodulin-binding protein 60 G-like isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
F4K2R69.5e-5139.64Calmodulin-binding protein 60 G OS=Arabidopsis thaliana OX=3702 GN=CBP60G PE=1 S... [more]
C0SVV61.6e-4536.39Calmodulin-binding protein 60 A OS=Arabidopsis thaliana OX=3702 GN=CBP60A PE=2 S... [more]
Q9FKL62.5e-4338.03Calmodulin-binding protein 60 B OS=Arabidopsis thaliana OX=3702 GN=CBP60B PE=2 S... [more]
C0SV511.2e-4237.10Calmodulin-binding protein 60 C OS=Arabidopsis thaliana OX=3702 GN=CBP60C PE=2 S... [more]
F4IPM31.6e-4237.68Calmodulin-binding protein 60 E OS=Arabidopsis thaliana OX=3702 GN=CBP60E PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A1S3BQ839.5e-8650.13calmodulin-binding protein 60 B-like OS=Cucumis melo OX=3656 GN=LOC103492515 PE=... [more]
A0A6J1HBN48.9e-8455.10calmodulin-binding protein 60 G-like isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1I0G55.7e-8354.76calmodulin-binding protein 60 G-like isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1HYX27.5e-8355.07calmodulin-binding protein 60 G-like isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1HDW81.7e-8255.56calmodulin-binding protein 60 G-like isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT5G26920.16.8e-5239.64Cam-binding protein 60-like G [more]
AT5G26920.21.5e-5139.29Cam-binding protein 60-like G [more]
AT5G62570.11.1e-4636.39Calmodulin binding protein-like [more]
AT5G62570.21.1e-4636.39Calmodulin binding protein-like [more]
AT5G57580.11.8e-4438.03Calmodulin-binding protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012416CALMODULIN-BINDING PROTEIN60PFAMPF07887Calmodulin_bindcoord: 129..366
e-value: 1.0E-72
score: 244.9
IPR012416CALMODULIN-BINDING PROTEIN60PANTHERPTHR31713OS02G0177800 PROTEINcoord: 81..367
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 375..420
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..28
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 375..397
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 398..420
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..31
NoneNo IPR availablePANTHERPTHR31713:SF43CALMODULIN-BINDING PROTEIN 60 Gcoord: 81..367

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0036432.1Lag0036432.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005516 calmodulin binding