Lag0036280 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0036280
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	50S ribosomal protein L18
Location	chr3: 43210772 .. 43211116 (+)
RNA-Seq Expression	Lag0036280
Synteny	Lag0036280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGATTCCTCCTCCAGTTAGGCCACCAAGATTAACCCAGTATCTAAAGCCATATGTCTTGAGGATGCACTTCACAAACAAGTATGTGAGCGCCCAGGTGATCCACTCCGAAACGGCCACCGTTGCTTCGGCTGCAAGCTCGCAAGAGAAGGCATTGCGGTCGAGCATGGAGTCAACGAGGGATGTGGCTGCTGCTGCAAAGATTGGGAAATTGTTGGGGGAGAGGCTTTTGCTTAAGGGAATCCCAGCTGTTTGTGTGCACTTGAAAAGAGAACAGAAATATCATGGTAAGGTCAAAGCTGTTGTTGATTCTGTTAGGGAAGCAGGTGTTAAGCTGCTTTGA

mRNA sequence

ATGGTGATTCCTCCTCCAGTTAGGCCACCAAGATTAACCCAGTATCTAAAGCCATATGTCTTGAGGATGCACTTCACAAACAAGTATGTGAGCGCCCAGGTGATCCACTCCGAAACGGCCACCGTTGCTTCGGCTGCAAGCTCGCAAGAGAAGGCATTGCGGTCGAGCATGGAGTCAACGAGGGATGTGGCTGCTGCTGCAAAGATTGGGAAATTGTTGGGGGAGAGGCTTTTGCTTAAGGGAATCCCAGCTGTTTGTGTGCACTTGAAAAGAGAACAGAAATATCATGGTAAGGTCAAAGCTGTTGTTGATTCTGTTAGGGAAGCAGGTGTTAAGCTGCTTTGA

Coding sequence (CDS)

ATGGTGATTCCTCCTCCAGTTAGGCCACCAAGATTAACCCAGTATCTAAAGCCATATGTCTTGAGGATGCACTTCACAAACAAGTATGTGAGCGCCCAGGTGATCCACTCCGAAACGGCCACCGTTGCTTCGGCTGCAAGCTCGCAAGAGAAGGCATTGCGGTCGAGCATGGAGTCAACGAGGGATGTGGCTGCTGCTGCAAAGATTGGGAAATTGTTGGGGGAGAGGCTTTTGCTTAAGGGAATCCCAGCTGTTTGTGTGCACTTGAAAAGAGAACAGAAATATCATGGTAAGGTCAAAGCTGTTGTTGATTCTGTTAGGGAAGCAGGTGTTAAGCTGCTTTGA

Protein sequence

MVIPPPVRPPRLTQYLKPYVLRMHFTNKYVSAQVIHSETATVASAASSQEKALRSSMESTRDVAAAAKIGKLLGERLLLKGIPAVCVHLKREQKYHGKVKAVVDSVREAGVKLL

Homology

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BLAST of Lag0036280 vs. NCBI nr
Match: KAG6590442.1 (hypothetical protein SDJN03_15865, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023978.1 rplR, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 210.7 bits (535), Expect = 6.7e-51
Identity = 111/114 (97.37%), Postives = 114/114 (100.00%), Query Frame = 0


Query: 1   MVIPPPVRPPRLTQYLKPYVLRMHFTNKYVSAQVIHSETATVASAASSQEKALRSSMEST 60
           MVIPPPVRPPRLTQYLKPYVL+MHFTNKYVSAQVIHSETATVASAASSQEKALRSSMEST
Sbjct: 1   MVIPPPVRPPRLTQYLKPYVLKMHFTNKYVSAQVIHSETATVASAASSQEKALRSSMEST 60

Query: 61  RDVAAAAKIGKLLGERLLLKGIPAVCVHLKREQKYHGKVKAVVDSVREAGVKLL 115
           RDVAAAAKIGKLLGERL+LKGIPAVCVHLKREQKYHGKVKAVVDSVREAG+KLL
Sbjct: 61  RDVAAAAKIGKLLGERLMLKGIPAVCVHLKREQKYHGKVKAVVDSVREAGIKLL 114

[Close]

BLAST of Lag0036280 vs. NCBI nr
Match: XP_022961444.1 (uncharacterized protein LOC111462025 [Cucurbita moschata] >XP_022961445.1 uncharacterized protein LOC111462025 [Cucurbita moschata])

HSP 1 Score: 210.3 bits (534), Expect = 8.7e-51
Identity = 111/114 (97.37%), Postives = 114/114 (100.00%), Query Frame = 0


Query: 1   MVIPPPVRPPRLTQYLKPYVLRMHFTNKYVSAQVIHSETATVASAASSQEKALRSSMEST 60
           MVIPPPVRPPRLTQYLKPYVL+MHFTNKYVSAQVIHSETATVASAASSQEKALRSSMEST
Sbjct: 1   MVIPPPVRPPRLTQYLKPYVLKMHFTNKYVSAQVIHSETATVASAASSQEKALRSSMEST 60

Query: 61  RDVAAAAKIGKLLGERLLLKGIPAVCVHLKREQKYHGKVKAVVDSVREAGVKLL 115
           RDVAAAAKIGKLLGERL+LKGIPAVCVHLKREQKYHGKVKAVVDSVREAG+KLL
Sbjct: 61  RDVAAAAKIGKLLGERLVLKGIPAVCVHLKREQKYHGKVKAVVDSVREAGIKLL 114

[Close]

BLAST of Lag0036280 vs. NCBI nr
Match: XP_022968817.1 (uncharacterized protein LOC111467940 [Cucurbita maxima] >XP_022968819.1 uncharacterized protein LOC111467940 [Cucurbita maxima] >XP_022968820.1 uncharacterized protein LOC111467940 [Cucurbita maxima])

HSP 1 Score: 209.1 bits (531), Expect = 1.9e-50
Identity = 110/114 (96.49%), Postives = 113/114 (99.12%), Query Frame = 0


Query: 1   MVIPPPVRPPRLTQYLKPYVLRMHFTNKYVSAQVIHSETATVASAASSQEKALRSSMEST 60
           MVIPPPVRPPRLTQYLKPYVL+MHFTNKYVSAQVIHSETATVASAASSQEK LRSSMEST
Sbjct: 1   MVIPPPVRPPRLTQYLKPYVLKMHFTNKYVSAQVIHSETATVASAASSQEKVLRSSMEST 60

Query: 61  RDVAAAAKIGKLLGERLLLKGIPAVCVHLKREQKYHGKVKAVVDSVREAGVKLL 115
           RDVAAAAKIGKLLGERL+LKGIPAVCVHLKREQKYHGKVKAVVDSVREAG+KLL
Sbjct: 61  RDVAAAAKIGKLLGERLMLKGIPAVCVHLKREQKYHGKVKAVVDSVREAGIKLL 114

[Close]

BLAST of Lag0036280 vs. NCBI nr
Match: XP_023515963.1 (uncharacterized protein LOC111779971 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 208.8 bits (530), Expect = 2.5e-50
Identity = 109/114 (95.61%), Postives = 114/114 (100.00%), Query Frame = 0


Query: 1   MVIPPPVRPPRLTQYLKPYVLRMHFTNKYVSAQVIHSETATVASAASSQEKALRSSMEST 60
           MVIPPPVRPPRLTQYLKPY+L+MHFTNKYVSAQVIHS+TATVASAASSQEKALRSSMEST
Sbjct: 1   MVIPPPVRPPRLTQYLKPYILKMHFTNKYVSAQVIHSKTATVASAASSQEKALRSSMEST 60

Query: 61  RDVAAAAKIGKLLGERLLLKGIPAVCVHLKREQKYHGKVKAVVDSVREAGVKLL 115
           RDVAAAAKIGKLLGERL+LKGIPAVCVHLKREQKYHGKVKAVVDSVREAG+KLL
Sbjct: 61  RDVAAAAKIGKLLGERLMLKGIPAVCVHLKREQKYHGKVKAVVDSVREAGIKLL 114

[Close]

BLAST of Lag0036280 vs. NCBI nr
Match: XP_022970166.1 (uncharacterized protein LOC111469142 [Cucurbita maxima] >XP_022970175.1 uncharacterized protein LOC111469142 [Cucurbita maxima])

HSP 1 Score: 206.8 bits (525), Expect = 9.6e-50
Identity = 107/114 (93.86%), Postives = 112/114 (98.25%), Query Frame = 0


Query: 1   MVIPPPVRPPRLTQYLKPYVLRMHFTNKYVSAQVIHSETATVASAASSQEKALRSSMEST 60
           MVIPPPVRPPRLTQYLKPYVL+MHFTNKYVSAQVIHSETAT+ASAASSQEKALR SMEST
Sbjct: 1   MVIPPPVRPPRLTQYLKPYVLKMHFTNKYVSAQVIHSETATIASAASSQEKALRPSMEST 60

Query: 61  RDVAAAAKIGKLLGERLLLKGIPAVCVHLKREQKYHGKVKAVVDSVREAGVKLL 115
           RDVAAA KIGKLLGERLLLKGIPAVCVHLKREQKYHGK+KAV+DSVREAG+KLL
Sbjct: 61  RDVAAAVKIGKLLGERLLLKGIPAVCVHLKREQKYHGKIKAVIDSVREAGIKLL 114

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BLAST of Lag0036280 vs. ExPASy Swiss-Prot
Match: A8IAP8 (50S ribosomal protein L18 OS=Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571) OX=438753 GN=rplR PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 5.9e-10
Identity = 41/92 (44.57%), Postives = 57/92 (61.96%), Query Frame = 0


Query: 21  LRMHFTNKYVSAQVIHSETATVASAASSQEKALRSSMESTRDVAAAAKIGKLLGERLLLK 80
           L +H T+K++ AQ+I         AASS EK LRSS+++  DV AA  IGKL+ ER   K
Sbjct: 29  LSVHRTSKHIYAQIIDDAKGETLVAASSLEKDLRSSLKTGADVEAAKAIGKLVAERATAK 88

Query: 81  GIPAVCVHLKREQKYHGKVKAVVDSVREAGVK 113
           G+ AV V  +    YHG+VKA+ +  RE G++
Sbjct: 89  GVTAV-VFDRGAYIYHGRVKALAEGAREGGLQ 119

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BLAST of Lag0036280 vs. ExPASy Swiss-Prot
Match: P52863 (50S ribosomal protein L18 OS=Vibrio proteolyticus OX=671 GN=rplR PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 7.8e-10
Identity = 41/92 (44.57%), Postives = 59/92 (64.13%), Query Frame = 0


Query: 21  LRMHFTNKYVSAQVIHSETATVASAASSQEKALRSSMESTRDVAAAAKIGKLLGERLLLK 80
           L +H T ++V AQVI S  + V +AAS+ EKA+R  +++T +V AA  +GK + ER L K
Sbjct: 26  LVVHRTPRHVYAQVIASNGSEVIAAASTVEKAIREQVKNTGNVDAAKAVGKAIAERALEK 85

Query: 81  GIPAVCVHLKREQKYHGKVKAVVDSVREAGVK 113
           G+  V       Q YHG+V A+ +S REAG+K
Sbjct: 86  GVETVAFDRSGFQ-YHGRVAALAESAREAGLK 116

[Close]

BLAST of Lag0036280 vs. ExPASy Swiss-Prot
Match: B7VLE1 (50S ribosomal protein L18 OS=Vibrio atlanticus (strain LGP32) OX=575788 GN=rplR PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.3e-09
Identity = 40/92 (43.48%), Postives = 60/92 (65.22%), Query Frame = 0


Query: 21  LRMHFTNKYVSAQVIHSETATVASAASSQEKALRSSMESTRDVAAAAKIGKLLGERLLLK 80
           L +H T ++V AQVI +  + V +AAS+ EKA+R  +++T +V AA  +GK + ER L K
Sbjct: 26  LVVHRTPRHVYAQVISANGSEVIAAASTVEKAIREQVKNTGNVDAAKAVGKAVAERALEK 85

Query: 81  GIPAVCVHLKREQKYHGKVKAVVDSVREAGVK 113
           G+ +V       Q YHG+V A+ +S REAG+K
Sbjct: 86  GVASVAFDRSGFQ-YHGRVAALAESAREAGLK 116

[Close]

BLAST of Lag0036280 vs. ExPASy Swiss-Prot
Match: B6EPU1 (50S ribosomal protein L18 OS=Aliivibrio salmonicida (strain LFI1238) OX=316275 GN=rplR PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 1.7e-09
Identity = 42/92 (45.65%), Postives = 57/92 (61.96%), Query Frame = 0


Query: 21  LRMHFTNKYVSAQVIHSETATVASAASSQEKALRSSMESTRDVAAAAKIGKLLGERLLLK 80
           L +H T ++  AQVI    + V +AAS+ EKA+R  + +T + AAA  IGKL+ ER + K
Sbjct: 26  LVVHRTPRHTYAQVIAPNGSEVIAAASTVEKAIREQVGNTSNKAAAVAIGKLIAERAIEK 85

Query: 81  GIPAVCVHLKREQKYHGKVKAVVDSVREAGVK 113
           GI  V       Q YHG+V A+ DS REAG+K
Sbjct: 86  GITNVAFDRSGFQ-YHGRVAALADSAREAGLK 116

[Close]

BLAST of Lag0036280 vs. ExPASy Swiss-Prot
Match: A7MWH4 (50S ribosomal protein L18 OS=Vibrio campbellii (strain ATCC BAA-1116 / BB120) OX=338187 GN=rplR PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 1.7e-09
Identity = 41/92 (44.57%), Postives = 59/92 (64.13%), Query Frame = 0


Query: 21  LRMHFTNKYVSAQVIHSETATVASAASSQEKALRSSMESTRDVAAAAKIGKLLGERLLLK 80
           L +H T ++V AQVI +  + V +AAS+ EKA+R  ++ T +V AA  +GK + ER L K
Sbjct: 26  LVVHRTPRHVYAQVIAANGSEVIAAASTVEKAIREQVKYTGNVDAAKAVGKAVAERALEK 85

Query: 81  GIPAVCVHLKREQKYHGKVKAVVDSVREAGVK 113
           G+ AV       Q YHG+V A+ +S REAG+K
Sbjct: 86  GVTAVAFDRSGFQ-YHGRVAALAESAREAGLK 116

The following BLAST results are available for this feature:

BLAST of Lag0036280 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6590442.1	6.7e-51	97.37	hypothetical protein SDJN03_15865, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022961444.1	8.7e-51	97.37	uncharacterized protein LOC111462025 [Cucurbita moschata] >XP_022961445.1 unchar...	[more]
XP_022968817.1	1.9e-50	96.49	uncharacterized protein LOC111467940 [Cucurbita maxima] >XP_022968819.1 uncharac...	[more]
XP_023515963.1	2.5e-50	95.61	uncharacterized protein LOC111779971 [Cucurbita pepo subsp. pepo]	[more]
XP_022970166.1	9.6e-50	93.86	uncharacterized protein LOC111469142 [Cucurbita maxima] >XP_022970175.1 uncharac...	[more]

BLAST of Lag0036280 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A8IAP8	5.9e-10	44.57	50S ribosomal protein L18 OS=Azorhizobium caulinodans (strain ATCC 43989 / DSM 5...	[more]
P52863	7.8e-10	44.57	50S ribosomal protein L18 OS=Vibrio proteolyticus OX=671 GN=rplR PE=3 SV=1	[more]
B7VLE1	1.3e-09	43.48	50S ribosomal protein L18 OS=Vibrio atlanticus (strain LGP32) OX=575788 GN=rplR ...	[more]
B6EPU1	1.7e-09	45.65	50S ribosomal protein L18 OS=Aliivibrio salmonicida (strain LFI1238) OX=316275 G...	[more]
A7MWH4	1.7e-09	44.57	50S ribosomal protein L18 OS=Vibrio campbellii (strain ATCC BAA-1116 / BB120) OX...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005484	Ribosomal protein L18	PFAM	PF00861	Ribosomal_L18p	coord: 20..113 e-value: 3.0E-17 score: 63.0
IPR005484	Ribosomal protein L18	PANTHER	PTHR12899	39S RIBOSOMAL PROTEIN L18, MITOCHONDRIAL	coord: 1..114
None	No IPR available	GENE3D	3.30.420.100		coord: 10..113 e-value: 4.6E-25 score: 90.3
None	No IPR available	PANTHER	PTHR12899:SF6	OS03G0694800 PROTEIN	coord: 1..114
None	No IPR available	CDD	cd00432	Ribosomal_L18_L5e	coord: 15..111 e-value: 2.10683E-26 score: 92.2179
None	No IPR available	SUPERFAMILY	53137	Translational machinery components	coord: 10..111

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0036280.1	Lag0036280.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006412	translation
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005840	ribosome
molecular_function	GO:0008097	5S rRNA binding
molecular_function	GO:0003735	structural constituent of ribosome