Lag0036267 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0036267
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	V-type proton ATPase subunit F
Location	chr3: 42863619 .. 42864446 (-)
RNA-Seq Expression	Lag0036267
Synteny	Lag0036267

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGGTAAAGCTCAAATCCCTACCAAAAGCTCAGCGCTTATTTCTATGATTGCTGATGAGGTACGCCCAATATACTTCCATAGTGCAACAGCTTTTCTGGGTACCTTAAACTATTAAATTGACTTGAGGTTCGATAGGAGTTAATATTACATGCTTATTTGATCTTATTGTTATGTGATTTTGGTGTTTGGTTTTCTTTCTACAGATAAATCATCCACTCATGTGATCTTATTGTTATGTCATATGTCATTGAAGTACCTCAAATACCTTTGTCTTAATCGGGTTTTACATTACATGTATGTGTATGTCAAAGAAAATCTGCTTGTCAATCACGTTTACATAAGTAGTTAGTCTTGAGCCCTAGAGTAAACTCTTTATTATTAGTCTTTCCATGAAATTGTCAACTTTGCTCAATAAACATCCATCATTCAATTTTTTGCTTCTGTGATTTTAGGAATATTTTCTTCAGTTCATTGCCATTCCCAAGGCATAATAAAGCATCTGGATAATGATAATGATTGGGCTCCCGCTCATTCTGTTGTGTTGTGCATCGTGAATTATGTGAGTTTTGGTTCTATGTTTAAACTAAATTTTCTTCATAATAGGATACGGTGGTTGGATTTCTTCTTGCTGGAGTTGGTAATGTTGACCTGCGAAGAAAAACTAATTACCTTATAGTGGACTCAAGTAAGTATCCCTCTGATGTAAATTGCTTGCAACTCTGATTTGCCATTTGACTTGTAACTCTGGCATCCTATTTCGTTAGGCTCATCCTCTGTGTCTAAATCCTGTGAAGTTCCAATTGCTGAAATCCTTGTTATTTAA

mRNA sequence

ATGGCTGGTAAAGCTCAAATCCCTACCAAAAGCTCAGCGCTTATTTCTATGATTGCTGATGAGGATACGGTGGTTGGATTTCTTCTTGCTGGAGTTGGTAATGTTGACCTGCGAAGAAAAACTAATTACCTTATAGTGGACTCAAGCTCATCCTCTGTGTCTAAATCCTGTGAAGTTCCAATTGCTGAAATCCTTGTTATTTAA

Coding sequence (CDS)

ATGGCTGGTAAAGCTCAAATCCCTACCAAAAGCTCAGCGCTTATTTCTATGATTGCTGATGAGGATACGGTGGTTGGATTTCTTCTTGCTGGAGTTGGTAATGTTGACCTGCGAAGAAAAACTAATTACCTTATAGTGGACTCAAGCTCATCCTCTGTGTCTAAATCCTGTGAAGTTCCAATTGCTGAAATCCTTGTTATTTAA

Protein sequence

MAGKAQIPTKSSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSSSSSVSKSCEVPIAEILVI

Homology

[Close]

BLAST of Lag0036267 vs. NCBI nr
Match: XP_022150945.1 (V-type proton ATPase subunit F [Momordica charantia])

HSP 1 Score: 94.0 bits (232), Expect = 5.4e-16
Identity = 48/51 (94.12%), Postives = 50/51 (98.04%), Query Frame = 0


Query: 1  MAGKAQIPTKSSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSSSS 52
          MAGKAQIPTKSSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDS ++
Sbjct: 1  MAGKAQIPTKSSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTT 51

[Close]

BLAST of Lag0036267 vs. NCBI nr
Match: XP_038879624.1 (V-type proton ATPase subunit F [Benincasa hispida])

HSP 1 Score: 92.8 bits (229), Expect = 1.2e-15
Identity = 47/51 (92.16%), Postives = 50/51 (98.04%), Query Frame = 0


Query: 1  MAGKAQIPTKSSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSSSS 52
          MAGKAQIPTK+SALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDS ++
Sbjct: 1  MAGKAQIPTKNSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTT 51

[Close]

BLAST of Lag0036267 vs. NCBI nr
Match: KAG6590444.1 (V-type proton ATPase subunit F, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 92.8 bits (229), Expect = 1.2e-15
Identity = 47/51 (92.16%), Postives = 50/51 (98.04%), Query Frame = 0


Query: 1  MAGKAQIPTKSSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSSSS 52
          MAGKAQIPTK+SALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDS ++
Sbjct: 24 MAGKAQIPTKNSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTT 74

[Close]

BLAST of Lag0036267 vs. NCBI nr
Match: XP_004151178.1 (V-type proton ATPase subunit F [Cucumis sativus] >XP_008450453.1 PREDICTED: V-type proton ATPase subunit F [Cucumis melo] >XP_008450454.1 PREDICTED: V-type proton ATPase subunit F [Cucumis melo] >XP_011660114.1 V-type proton ATPase subunit F [Cucumis sativus] >KAA0040144.1 V-type proton ATPase subunit F-like [Cucumis melo var. makuwa] >KGN66455.1 hypothetical protein Csa_007359 [Cucumis sativus] >TYK27648.1 V-type proton ATPase subunit F-like [Cucumis melo var. makuwa])

HSP 1 Score: 92.8 bits (229), Expect = 1.2e-15
Identity = 47/51 (92.16%), Postives = 50/51 (98.04%), Query Frame = 0


Query: 1  MAGKAQIPTKSSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSSSS 52
          MAGKAQIPTK+SALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDS ++
Sbjct: 1  MAGKAQIPTKNSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTT 51

[Close]

BLAST of Lag0036267 vs. NCBI nr
Match: XP_022930111.1 (V-type proton ATPase subunit F isoform X2 [Cucurbita moschata] >XP_022930112.1 V-type proton ATPase subunit F isoform X2 [Cucurbita moschata] >XP_022961522.1 V-type proton ATPase subunit F [Cucurbita moschata] >XP_022968815.1 V-type proton ATPase subunit F [Cucurbita maxima] >XP_022973615.1 V-type proton ATPase subunit F isoform X1 [Cucurbita maxima] >XP_022973616.1 V-type proton ATPase subunit F isoform X1 [Cucurbita maxima] >XP_023522885.1 V-type proton ATPase subunit F [Cucurbita pepo subsp. pepo] >XP_023553409.1 V-type proton ATPase subunit F isoform X1 [Cucurbita pepo subsp. pepo] >XP_023553415.1 V-type proton ATPase subunit F isoform X1 [Cucurbita pepo subsp. pepo] >KAG7023981.1 V-type proton ATPase subunit F [Cucurbita argyrosperma subsp. argyrosperma] >KAG7032475.1 V-type proton ATPase subunit F, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 92.8 bits (229), Expect = 1.2e-15
Identity = 47/51 (92.16%), Postives = 50/51 (98.04%), Query Frame = 0


Query: 1  MAGKAQIPTKSSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSSSS 52
          MAGKAQIPTK+SALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDS ++
Sbjct: 1  MAGKAQIPTKNSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTT 51

[Close]

BLAST of Lag0036267 vs. ExPASy Swiss-Prot
Match: Q9ZQX4 (V-type proton ATPase subunit F OS=Arabidopsis thaliana OX=3702 GN=VHA-F PE=2 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 9.5e-16
Identity = 40/51 (78.43%), Postives = 48/51 (94.12%), Query Frame = 0


Query: 1  MAGKAQIPTKSSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSSSS 52
          MAG+A IP ++SALI+MIADEDTVVGFL+AGVGNVD+RRKTNYLIVDS ++
Sbjct: 1  MAGRATIPARNSALIAMIADEDTVVGFLMAGVGNVDIRRKTNYLIVDSKTT 51

[Close]

BLAST of Lag0036267 vs. ExPASy Swiss-Prot
Match: Q55AH5 (V-type proton ATPase subunit F OS=Dictyostelium discoideum OX=44689 GN=vatF PE=3 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 1.5e-05
Identity = 22/40 (55.00%), Postives = 31/40 (77.50%), Query Frame = 0


Query: 12 SALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSSSS 52
          +AL+++I DED V GFLLAGVG  D ++  N+L+VDS +S
Sbjct: 9  TALVAVIGDEDVVTGFLLAGVGQKDKKKNENFLVVDSKTS 48

[Close]

BLAST of Lag0036267 vs. ExPASy Swiss-Prot
Match: Q23680 (Probable V-type proton ATPase subunit F OS=Caenorhabditis elegans OX=6239 GN=vha-9 PE=3 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 2.0e-05
Identity = 22/38 (57.89%), Postives = 30/38 (78.95%), Query Frame = 0


Query: 14 LISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSSSS 52
          ++++I DEDTVVGFLL GVG ++  RK NYLIVD  ++
Sbjct: 9  ILAVIGDEDTVVGFLLGGVGELNKARKPNYLIVDKQTT 46

[Close]

BLAST of Lag0036267 vs. ExPASy Swiss-Prot
Match: Q24583 (V-type proton ATPase subunit F 1 OS=Drosophila melanogaster OX=7227 GN=Vha14-1 PE=1 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 1.3e-04
Identity = 20/38 (52.63%), Postives = 29/38 (76.32%), Query Frame = 0


Query: 14 LISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSSSS 52
          LIS+I DEDT VGFLL GVG ++  R  N+++VD +++
Sbjct: 11 LISVIGDEDTCVGFLLGGVGEINKNRHPNFMVVDKNTA 48

[Close]

BLAST of Lag0036267 vs. ExPASy Swiss-Prot
Match: Q17029 (V-type proton ATPase subunit F OS=Anopheles gambiae OX=7165 GN=Vha14 PE=2 SV=2)

HSP 1 Score: 46.6 bits (109), Expect = 1.3e-04
Identity = 21/47 (44.68%), Postives = 31/47 (65.96%), Query Frame = 0


Query: 5  AQIPTKSSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSSSS 52
          A +      LIS+I DEDT VGFLL GVG ++  R  N+++VD +++
Sbjct: 2  ALLSAMKGKLISVIGDEDTCVGFLLGGVGEINKNRHPNFMVVDKNTA 48

The following BLAST results are available for this feature:

BLAST of Lag0036267 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150945.1	5.4e-16	94.12	V-type proton ATPase subunit F [Momordica charantia]	[more]
XP_038879624.1	1.2e-15	92.16	V-type proton ATPase subunit F [Benincasa hispida]	[more]
KAG6590444.1	1.2e-15	92.16	V-type proton ATPase subunit F, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_004151178.1	1.2e-15	92.16	V-type proton ATPase subunit F [Cucumis sativus] >XP_008450453.1 PREDICTED: V-ty...	[more]
XP_022930111.1	1.2e-15	92.16	V-type proton ATPase subunit F isoform X2 [Cucurbita moschata] >XP_022930112.1 V...	[more]

BLAST of Lag0036267 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZQX4	9.5e-16	78.43	V-type proton ATPase subunit F OS=Arabidopsis thaliana OX=3702 GN=VHA-F PE=2 SV=...	[more]
Q55AH5	1.5e-05	55.00	V-type proton ATPase subunit F OS=Dictyostelium discoideum OX=44689 GN=vatF PE=3...	[more]
Q23680	2.0e-05	57.89	Probable V-type proton ATPase subunit F OS=Caenorhabditis elegans OX=6239 GN=vha...	[more]
Q24583	1.3e-04	52.63	V-type proton ATPase subunit F 1 OS=Drosophila melanogaster OX=7227 GN=Vha14-1 P...	[more]
Q17029	1.3e-04	44.68	V-type proton ATPase subunit F OS=Anopheles gambiae OX=7165 GN=Vha14 PE=2 SV=2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036906	ATPase, V1 complex, subunit F superfamily	GENE3D	3.40.50.10580	ATPase, V1 complex, subunit F	coord: 8..64 e-value: 2.7E-12 score: 48.8
IPR008218	ATPase, V1 complex, subunit F	PFAM	PF01990	ATP-synt_F	coord: 15..42 e-value: 4.9E-5 score: 23.6
None	No IPR available	PANTHER	PTHR13861:SF12	V-TYPE PROTON ATPASE SUBUNIT F	coord: 1..51
None	No IPR available	PANTHER	PTHR13861	VACUOLAR ATP SYNTHASE SUBUNIT F	coord: 1..51

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0036267.1	Lag0036267.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1902600	proton transmembrane transport
biological_process	GO:0034220	ion transmembrane transport
cellular_component	GO:0033180	proton-transporting V-type ATPase, V1 domain
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0046961	proton-transporting ATPase activity, rotational mechanism