Lag0036250 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0036250
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionprotein AUXIN RESPONSE 4
Locationchr3: 42500079 .. 42502715 (+)
RNA-Seq ExpressionLag0036250
SyntenyLag0036250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGACCATTACCGAAGAACCAGACGAGCCAGTGCCGAAGCCATCAAAGCCCAAAACCAACAAATCAAGTTCATCCTCGAAGCCACCGCCGAGCACCGCCGCCGCAACCCCCAAAACCCCTTCTTCTCCGACCCCAAATCCGTTCACCTTCTGGTTCTATTTCACTTTGATCGTCTCTATCATAACCTTTTTCTTCATCTCTCTCCCTTCTCTCTCTCCCCAAGACCCCAAATCCTGGTTCTTAAGCTTACCCAACAGCCTCCGTCACCATTACTCAAAGGGCCGTTTTCTTAAGGTACAAATTAGCCCTAATTTATCTCCAATTGAAGTTTTCGCCGTTGAAAATGGCGCAAAAGGGAACGAAAATGTGGTCATTGTTCATGGGCTGGGGCTGAGCTCTTACTCCTTCCGCAAGGTTTTGGACTCATTAGGCTCCAAAGGAGTCCGGGCTTTGGCGTTTGATCTACCCGGCAATGGCTTTTCTGACAAATCCACGGTTGAGATCGACGAGAGCACAAATGGGGTTTTAGGGAGGCTTTTGGATGTTTATAATTTGATCCATGAAAAGGGTATCTTCTGGGCGTTTGATCAGATCATTGAAACTGGGCAGATTCCTTATGAAGAAATTCAGAAACATGTTCCGAAGAGGAAGATTTTGAAGCCAATTGGGTTAGGTCCTGAAGAGATTAGCAGTATTTTGGGGCAAATCATTGATACTATTGGTTTGGCTCCTGTGCATTTGGTTCTTCATGATTCAGCTTTATCAATGGCGGGATATTGGGTTGCAGAGAATTCAGGATTTGTGAGGAGTTTAACTCTTATTGATACCTTATCGAAACCTTCTCTTCCTTTATGGCTCTTGGAGTTGCCTGTTGTGAGAGAGGTTATTTTGGGATCTAATTTTGTGTATTCAAGGTTGATCAACTTCTGTTGTTCTAAAGGGAATGATGCTGCCTTGGATGTGGAGGCTCATAGAGTTCTTTTGAAGGGCCGGGGTGGGCGGAGGGCGGTTGTTAGCATGGGGAAGAAGTTGAACCATAGCTTTGACATTGCAGAATGGGGTGGTTTGGATGATCTGAAAAGTGTGCCGATTCAAGTTATTTGGTCCAACGGTTGGTCAAATGAATGGAGCGTGGAGGGACGCCGGGTTGCTGATGTGCTTCCACAGGCAGCTTTTGTTAAACATTCCGGTGGACGGTGGGCTCAGGTAAGGATTCATTACTTTGATTGAGAAGTTGTAATGAGTTTGGCTTGTATTTTATGTAATGAATTTGCAGTCTATATTGTGTTTTGCTGAGTTCAGAAGTATCAGTAGTCCTTTTGAGGATGAGGATTTTCATGGATTTAAATGAAACATGAATGATATAGTTAGGCCTGCCTACAGAAGCTGTTTTAGCATTCGAGTTCGAATTTTCATATCTTAGAACCATCACTGTTTTTCTCTAAAGTACAATGTGAAAAGTTTGAGTGACGTAGGGTGTATAATATGCAGCTTAACAAAATTAAAAGAAATCTGTTACTTCGAAATTTAACATTATCAAGCAGGACATGTCAAGCATGAAGGTTGTTCAAAATTTCAACAAACATCAAACTTCTAAGTAGGAAGTTGGAGTCCTTTGGATTCAATTCTGCTAGACTAGGTGAAAGTAAAACTTAGAGAGGGGCTAGGGAGGGGAGAGAAATGAGTGATGTGACACCGGATAACTTAGTGAGGAAGCATAGGCTCGGCTAATGAGGAGTTTGAGATGATGTTTCTGTCAACCAGCAGTCGAGATGGTGGAAAAAGTTTTCTTTTCTAGCTTTACAGAGAAAGTTACATAGACATATAGACTCACAGTTGTGCTTGCTGAACTGGAGATAGTAGAGAGTTGGGAAGAGACGCTCAAGACATCAACCCCCTGTTTAGATCATATGAGAGTCAAAACAGAAGGAAATGAAAAATGTTGACTTGTACCCATATGAACACTATATTTGAGACATCGTGAGTGCTTAAGCGTTGATTGTAAGAGATTACTTAGTCTCTCGGTGCAAAGAGGGTCACTTTTTGGCTTGGAGAGAACCTATTAAAACTCTCCCTAATCCATTCAGTTTGTCATCATGATTGTTTAACTTGTCCATGGTAACAAGTTGATTTCATCTGTCTTTGTGGATTGGCTCCTTGATTTTCATTTGTTTCCATAAATGGCCTCTCTTAGTGAAAGTTTATGTCATCTCCTTATTTTGGTACTTGGATGTTTGATATCACATTTTGCTCGATATCTTTGTCAATGTTTCTTTAATGGGAATCATGACTATTGATCGAACATAAGTGGGACTATTTTCATGCTAGAATTGCTACCCAGAAAGCTTGAATATATGTCTCTCTTTGAAGTTCAAATAAGCTCTTTATCTTTCTTGACCTTAGGAAGATGCAGCCAATGTCGTAGCTGACAGTATAGCTCAGTTTGTGTCCTCATTACCCCCCACGGTCAGGAAAACTGTCGAAGAGCCCGAACACATTCAGAAAGTTTTTGATGAATTGGGGAACAACGATGACCACCACCACGATCACCATCACCACCACGACCATGGCAGCCACGGTATTCATGGAGCTGGCTACATGGACGGCTATGGGCTCGGCGGTCATGCATGGTGA

mRNA sequence

ATGGTGACCATTACCGAAGAACCAGACGAGCCAGTGCCGAAGCCATCAAAGCCCAAAACCAACAAATCAAGTTCATCCTCGAAGCCACCGCCGAGCACCGCCGCCGCAACCCCCAAAACCCCTTCTTCTCCGACCCCAAATCCGTTCACCTTCTGGTTCTATTTCACTTTGATCGTCTCTATCATAACCTTTTTCTTCATCTCTCTCCCTTCTCTCTCTCCCCAAGACCCCAAATCCTGGTTCTTAAGCTTACCCAACAGCCTCCGTCACCATTACTCAAAGGGCCGTTTTCTTAAGGTACAAATTAGCCCTAATTTATCTCCAATTGAAGTTTTCGCCGTTGAAAATGGCGCAAAAGGGAACGAAAATGTGGTCATTGTTCATGGGCTGGGGCTGAGCTCTTACTCCTTCCGCAAGGTTTTGGACTCATTAGGCTCCAAAGGAGTCCGGGCTTTGGCGTTTGATCTACCCGGCAATGGCTTTTCTGACAAATCCACGGTTGAGATCGACGAGAGCACAAATGGGGTTTTAGGGAGGCTTTTGGATGTTTATAATTTGATCCATGAAAAGGGTATCTTCTGGGCGTTTGATCAGATCATTGAAACTGGGCAGATTCCTTATGAAGAAATTCAGAAACATGTTCCGAAGAGGAAGATTTTGAAGCCAATTGGGTTAGGTCCTGAAGAGATTAGCAGTATTTTGGGGCAAATCATTGATACTATTGGTTTGGCTCCTGTGCATTTGGTTCTTCATGATTCAGCTTTATCAATGGCGGGATATTGGGTTGCAGAGAATTCAGGATTTGTGAGGAGTTTAACTCTTATTGATACCTTATCGAAACCTTCTCTTCCTTTATGGCTCTTGGAGTTGCCTGTTGTGAGAGAGGTTATTTTGGGATCTAATTTTGTGTATTCAAGGTTGATCAACTTCTGTTGTTCTAAAGGGAATGATGCTGCCTTGGATGTGGAGGCTCATAGAGTTCTTTTGAAGGGCCGGGGTGGGCGGAGGGCGGTTGTTAGCATGGGGAAGAAGTTGAACCATAGCTTTGACATTGCAGAATGGGGTGGTTTGGATGATCTGAAAAGTGTGCCGATTCAAGTTATTTGGTCCAACGGTTGGTCAAATGAATGGAGCGTGGAGGGACGCCGGGTTGCTGATGTGCTTCCACAGGCAGCTTTTGTTAAACATTCCGGTGGACGGTGGGCTCAGGAAGATGCAGCCAATGTCGTAGCTGACAGTATAGCTCAGTTTGTGTCCTCATTACCCCCCACGGTCAGGAAAACTGTCGAAGAGCCCGAACACATTCAGAAAGTTTTTGATGAATTGGGGAACAACGATGACCACCACCACGATCACCATCACCACCACGACCATGGCAGCCACGGTATTCATGGAGCTGGCTACATGGACGGCTATGGGCTCGGCGGTCATGCATGGTGA

Coding sequence (CDS)

ATGGTGACCATTACCGAAGAACCAGACGAGCCAGTGCCGAAGCCATCAAAGCCCAAAACCAACAAATCAAGTTCATCCTCGAAGCCACCGCCGAGCACCGCCGCCGCAACCCCCAAAACCCCTTCTTCTCCGACCCCAAATCCGTTCACCTTCTGGTTCTATTTCACTTTGATCGTCTCTATCATAACCTTTTTCTTCATCTCTCTCCCTTCTCTCTCTCCCCAAGACCCCAAATCCTGGTTCTTAAGCTTACCCAACAGCCTCCGTCACCATTACTCAAAGGGCCGTTTTCTTAAGGTACAAATTAGCCCTAATTTATCTCCAATTGAAGTTTTCGCCGTTGAAAATGGCGCAAAAGGGAACGAAAATGTGGTCATTGTTCATGGGCTGGGGCTGAGCTCTTACTCCTTCCGCAAGGTTTTGGACTCATTAGGCTCCAAAGGAGTCCGGGCTTTGGCGTTTGATCTACCCGGCAATGGCTTTTCTGACAAATCCACGGTTGAGATCGACGAGAGCACAAATGGGGTTTTAGGGAGGCTTTTGGATGTTTATAATTTGATCCATGAAAAGGGTATCTTCTGGGCGTTTGATCAGATCATTGAAACTGGGCAGATTCCTTATGAAGAAATTCAGAAACATGTTCCGAAGAGGAAGATTTTGAAGCCAATTGGGTTAGGTCCTGAAGAGATTAGCAGTATTTTGGGGCAAATCATTGATACTATTGGTTTGGCTCCTGTGCATTTGGTTCTTCATGATTCAGCTTTATCAATGGCGGGATATTGGGTTGCAGAGAATTCAGGATTTGTGAGGAGTTTAACTCTTATTGATACCTTATCGAAACCTTCTCTTCCTTTATGGCTCTTGGAGTTGCCTGTTGTGAGAGAGGTTATTTTGGGATCTAATTTTGTGTATTCAAGGTTGATCAACTTCTGTTGTTCTAAAGGGAATGATGCTGCCTTGGATGTGGAGGCTCATAGAGTTCTTTTGAAGGGCCGGGGTGGGCGGAGGGCGGTTGTTAGCATGGGGAAGAAGTTGAACCATAGCTTTGACATTGCAGAATGGGGTGGTTTGGATGATCTGAAAAGTGTGCCGATTCAAGTTATTTGGTCCAACGGTTGGTCAAATGAATGGAGCGTGGAGGGACGCCGGGTTGCTGATGTGCTTCCACAGGCAGCTTTTGTTAAACATTCCGGTGGACGGTGGGCTCAGGAAGATGCAGCCAATGTCGTAGCTGACAGTATAGCTCAGTTTGTGTCCTCATTACCCCCCACGGTCAGGAAAACTGTCGAAGAGCCCGAACACATTCAGAAAGTTTTTGATGAATTGGGGAACAACGATGACCACCACCACGATCACCATCACCACCACGACCATGGCAGCCACGGTATTCATGGAGCTGGCTACATGGACGGCTATGGGCTCGGCGGTCATGCATGGTGA

Protein sequence

MVTITEEPDEPVPKPSKPKTNKSSSSSKPPPSTAAATPKTPSSPTPNPFTFWFYFTLIVSIITFFFISLPSLSPQDPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAKGNENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKSTVEIDESTNGVLGRLLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEISSILGQIIDTIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILGSNFVYSRLINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDDLKSVPIQVIWSNGWSNEWSVEGRRVADVLPQAAFVKHSGGRWAQEDAANVVADSIAQFVSSLPPTVRKTVEEPEHIQKVFDELGNNDDHHHDHHHHHDHGSHGIHGAGYMDGYGLGGHAW
Homology
BLAST of Lag0036250 vs. NCBI nr
Match: XP_038880139.1 (protein AUXIN RESPONSE 4 [Benincasa hispida])

HSP 1 Score: 876.3 bits (2263), Expect = 1.2e-250
Identity = 442/481 (91.89%), Postives = 454/481 (94.39%), Query Frame = 0

Query: 1   MVTITEEPDEPVPKPSKPKTNKSSSSSKPPPSTAAATPKTPSSPTPNPFTFWFYFTLIVS 60
           MVTITEEPDE VPKPSKPK+NKSSSSSKPPPS+  A PKTP SPTPNPFTFWFYFTLIVS
Sbjct: 1   MVTITEEPDESVPKPSKPKSNKSSSSSKPPPSSTTAPPKTPPSPTPNPFTFWFYFTLIVS 60

Query: 61  IITFFFISLPSLSPQDPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAKG 120
            +TFFFISLPSLSPQDPKSWFLSLP SLR HYSKGR LKVQISPNLSPIEVFAVENG KG
Sbjct: 61  FVTFFFISLPSLSPQDPKSWFLSLPTSLRQHYSKGRLLKVQISPNLSPIEVFAVENGVKG 120

Query: 121 NENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKSTVEIDESTNGVLGRL 180
           NENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKST EIDES+NGVLGRL
Sbjct: 121 NENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKSTAEIDESSNGVLGRL 180

Query: 181 LDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEISSILGQIIDT 240
           LDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEI SILGQI+DT
Sbjct: 181 LDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEIGSILGQIVDT 240

Query: 241 IGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILGS 300
           IGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILGS
Sbjct: 241 IGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILGS 300

Query: 301 NFVYSRLINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDDL 360
           NFVYSRLIN CCSKGNDA +DVEAHRVLLKGRGGRRAVVSMGKKLN SFDI+EWGGLDDL
Sbjct: 301 NFVYSRLINLCCSKGNDAPIDVEAHRVLLKGRGGRRAVVSMGKKLNDSFDISEWGGLDDL 360

Query: 361 KSVPIQVIWSNGWSNEWSVEGRRVADVLPQAAFVKHSGGRWAQEDAANVVADSIAQFVSS 420
           KSVP+QVIWSNGWSNEWS EGRRVADVLPQA+FVKHSGGRWAQED A+VVADSIAQFVSS
Sbjct: 361 KSVPMQVIWSNGWSNEWSAEGRRVADVLPQASFVKHSGGRWAQEDVADVVADSIAQFVSS 420

Query: 421 LPPTVRKTVEE--PEHIQKVFDELGNNDDHHHDHHHHHDHGSHGIHGAGYMDGYGLGGHA 480
           LPPTVRK+ EE  PEHIQKVFDE GN+     DHHHHHDHGSHGIHGAGYMDGYGLG HA
Sbjct: 421 LPPTVRKSAEETTPEHIQKVFDESGNS-----DHHHHHDHGSHGIHGAGYMDGYGLGSHA 476

BLAST of Lag0036250 vs. NCBI nr
Match: XP_022969547.1 (protein AUXIN RESPONSE 4 [Cucurbita maxima])

HSP 1 Score: 859.8 bits (2220), Expect = 1.2e-245
Identity = 436/480 (90.83%), Postives = 448/480 (93.33%), Query Frame = 0

Query: 1   MVTITEEPDEPVPKPSKPKTNKSSSSSKPPP-STAAATPKTPSSPTPNPFTFWFYFTLIV 60
           MVTITEE DEPVPK SKPK NKSSSSSKPPP STAAAT KT  SP PNPFTFWFYFTLIV
Sbjct: 1   MVTITEESDEPVPKASKPKPNKSSSSSKPPPSSTAAATSKTLRSPNPNPFTFWFYFTLIV 60

Query: 61  SIITFFFISLPSLSPQDPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAK 120
           SIITFFFIS PSLSPQDPKSWFLSLP SLRHHYSKGR LKVQ+SPNLSPIEVFAVENGAK
Sbjct: 61  SIITFFFISFPSLSPQDPKSWFLSLPKSLRHHYSKGRLLKVQVSPNLSPIEVFAVENGAK 120

Query: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKSTVEIDESTNGVLGR 180
           GNENVV+VHGLGLSSYSFRKVLDSLGSKGVRA+AFDLPGNGFSDKST EIDES+NGV GR
Sbjct: 121 GNENVVVVHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKSTAEIDESSNGVFGR 180

Query: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEISSILGQIID 240
           LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVP+RKILKPIGLGPEEI SILGQIID
Sbjct: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPQRKILKPIGLGPEEIGSILGQIID 240

Query: 241 TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG 300
           T+GLAPVHLVLHDSALSMAGYWVAENS  VRSLTLIDTLS PSLPLWLLELPV REV+LG
Sbjct: 241 TVGLAPVHLVLHDSALSMAGYWVAENSQSVRSLTLIDTLSNPSLPLWLLELPVAREVVLG 300

Query: 301 SNFVYSRLINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDD 360
           SNFVYS+LINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDD
Sbjct: 301 SNFVYSKLINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDD 360

Query: 361 LKSVPIQVIWSNGWSNEWSVEGRRVADVLPQAAFVKHSGGRWAQEDAANVVADSIAQFVS 420
           LKSVPIQVIWSN WS+EWS EG RVAD+LPQAAFVKHSGGRWAQEDAAN VADSIAQFVS
Sbjct: 361 LKSVPIQVIWSNNWSDEWSAEGSRVADLLPQAAFVKHSGGRWAQEDAANAVADSIAQFVS 420

Query: 421 SLPPTVRKTVEEPEHIQKVFDELGNNDDHHHDHHHHHDHGSHGIHGAGYMDGYGLGGHAW 480
           SLPPTVRK VEEPEH QKVFDELGN+D     HHHHHDHGSH IHGAGYM+GYGLG  AW
Sbjct: 421 SLPPTVRKDVEEPEHTQKVFDELGNSD----QHHHHHDHGSHDIHGAGYMEGYGLGSQAW 476

BLAST of Lag0036250 vs. NCBI nr
Match: KAG6588477.1 (Protein AUXIN RESPONSE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 855.9 bits (2210), Expect = 1.7e-244
Identity = 431/480 (89.79%), Postives = 448/480 (93.33%), Query Frame = 0

Query: 1   MVTITEEPDEPVPKPSKPKTNKSSSSSKPPP-STAAATPKTPSSPTPNPFTFWFYFTLIV 60
           MVTITEE DEPVPK SKPK NKSSSS+KPPP STAAAT KT  SP P+PFTFWFYFTLIV
Sbjct: 1   MVTITEESDEPVPKASKPKPNKSSSSTKPPPLSTAAATSKTLRSPNPHPFTFWFYFTLIV 60

Query: 61  SIITFFFISLPSLSPQDPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAK 120
           SIITFFFIS+PSLSPQDPKSWFLSLP SLRHHYSKGR LKVQISPNLSPIEVFAVENGAK
Sbjct: 61  SIITFFFISVPSLSPQDPKSWFLSLPKSLRHHYSKGRLLKVQISPNLSPIEVFAVENGAK 120

Query: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKSTVEIDESTNGVLGR 180
           GNENVV+VHGLGLSSYSFRKVLDSLGSKGVRA+AFDLPGNGFSDKST EIDE++NGV GR
Sbjct: 121 GNENVVVVHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKSTAEIDENSNGVFGR 180

Query: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEISSILGQIID 240
           LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVP+RKILKPIGLGPEEI SILGQIID
Sbjct: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPQRKILKPIGLGPEEIGSILGQIID 240

Query: 241 TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG 300
           T+GLAPVHLVLHDSALSMAGYWVAENS  VRSLTLIDTLS PSLPLWLLELPV RE++LG
Sbjct: 241 TVGLAPVHLVLHDSALSMAGYWVAENSQSVRSLTLIDTLSNPSLPLWLLELPVAREIVLG 300

Query: 301 SNFVYSRLINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDD 360
           SNFVYS+LINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDD
Sbjct: 301 SNFVYSKLINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDD 360

Query: 361 LKSVPIQVIWSNGWSNEWSVEGRRVADVLPQAAFVKHSGGRWAQEDAANVVADSIAQFVS 420
           LKSVPIQVIWSN WS+EWS EG RVAD+LPQAAFVKHSGGRWAQEDAAN + DSIAQFVS
Sbjct: 361 LKSVPIQVIWSNNWSDEWSAEGSRVADLLPQAAFVKHSGGRWAQEDAANAIVDSIAQFVS 420

Query: 421 SLPPTVRKTVEEPEHIQKVFDELGNNDDHHHDHHHHHDHGSHGIHGAGYMDGYGLGGHAW 480
           SLPPTVRK VEEPEH QKVFDELGN+D     HHHHHDHG H IHGAGYM+GYGLG HAW
Sbjct: 421 SLPPTVRKAVEEPEHTQKVFDELGNSD----QHHHHHDHGGHDIHGAGYMEGYGLGSHAW 476

BLAST of Lag0036250 vs. NCBI nr
Match: XP_022933511.1 (protein AUXIN RESPONSE 4 [Cucurbita moschata])

HSP 1 Score: 855.9 bits (2210), Expect = 1.7e-244
Identity = 432/480 (90.00%), Postives = 447/480 (93.12%), Query Frame = 0

Query: 1   MVTITEEPDEPVPKPSKPKTNKSSSSSKPPP-STAAATPKTPSSPTPNPFTFWFYFTLIV 60
           MVTITEE DEPVPK SKPK NKSSSSSKPPP STAAAT KT  SP PNPFTFWFYFTLIV
Sbjct: 1   MVTITEESDEPVPKASKPKPNKSSSSSKPPPSSTAAATSKTLRSPNPNPFTFWFYFTLIV 60

Query: 61  SIITFFFISLPSLSPQDPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAK 120
           SIITFFFIS+PSLSPQDPKSWFLSLP SLRHHYSKGR LKVQISPNLSPIEVFAVENGAK
Sbjct: 61  SIITFFFISVPSLSPQDPKSWFLSLPKSLRHHYSKGRLLKVQISPNLSPIEVFAVENGAK 120

Query: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKSTVEIDESTNGVLGR 180
           GNENVV+VHGLGLSSYSFRKVLDSLGSKGV A+AFDLPGNGFSDKST EIDE++NGV GR
Sbjct: 121 GNENVVVVHGLGLSSYSFRKVLDSLGSKGVHAVAFDLPGNGFSDKSTAEIDENSNGVFGR 180

Query: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEISSILGQIID 240
           LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVP+RKILKPIGLGPEEI SILGQIID
Sbjct: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPQRKILKPIGLGPEEIGSILGQIID 240

Query: 241 TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG 300
           T+GL+PVHLVLHDSALSMAGYWVAENS  VRSLTLIDTLS PSLPLWLLELPV RE++LG
Sbjct: 241 TVGLSPVHLVLHDSALSMAGYWVAENSQSVRSLTLIDTLSNPSLPLWLLELPVAREIVLG 300

Query: 301 SNFVYSRLINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDD 360
           SNFVYS+LINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDD
Sbjct: 301 SNFVYSKLINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDD 360

Query: 361 LKSVPIQVIWSNGWSNEWSVEGRRVADVLPQAAFVKHSGGRWAQEDAANVVADSIAQFVS 420
           LK VPIQVIWSN WS+EWS EG RVAD+LPQAAFVKHSGGRWAQEDAAN VADSIAQFVS
Sbjct: 361 LKRVPIQVIWSNNWSDEWSAEGSRVADLLPQAAFVKHSGGRWAQEDAANAVADSIAQFVS 420

Query: 421 SLPPTVRKTVEEPEHIQKVFDELGNNDDHHHDHHHHHDHGSHGIHGAGYMDGYGLGGHAW 480
           SLPPTVRK VEEPEH QKVFDELGN+D     HHHHHDHG H IHGAGYM+GYGLG HAW
Sbjct: 421 SLPPTVRKAVEEPEHTQKVFDELGNSD----QHHHHHDHGGHDIHGAGYMEGYGLGSHAW 476

BLAST of Lag0036250 vs. NCBI nr
Match: XP_023530238.1 (protein AUXIN RESPONSE 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 850.5 bits (2196), Expect = 7.0e-243
Identity = 429/480 (89.38%), Postives = 446/480 (92.92%), Query Frame = 0

Query: 1   MVTITEEPDEPVPKPSKPKTNKSSSSSK-PPPSTAAATPKTPSSPTPNPFTFWFYFTLIV 60
           MVTITEE D+PVPK SKPK NKSSSS+K PPPSTAAAT KT  SP PNPFTFWFYFTLIV
Sbjct: 1   MVTITEESDDPVPKASKPKPNKSSSSTKPPPPSTAAATSKTLRSPNPNPFTFWFYFTLIV 60

Query: 61  SIITFFFISLPSLSPQDPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAK 120
           SIITFFFIS PSLSPQDPKSWFLSLP SLRHHYSKGR LKVQ+SPNLSPIEVFAVENGAK
Sbjct: 61  SIITFFFISFPSLSPQDPKSWFLSLPKSLRHHYSKGRLLKVQVSPNLSPIEVFAVENGAK 120

Query: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKSTVEIDESTNGVLGR 180
           GNENVV+VHGLGLSSYSFRKVLDSLGSKGVRA+AFDLPGNGFSDKST EIDE++NGV GR
Sbjct: 121 GNENVVVVHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKSTAEIDENSNGVFGR 180

Query: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEISSILGQIID 240
           LLDVYNLIH+KGIFWAFDQIIETGQIPYEEIQKHVP+RKILKPIGLGPEEI SILGQIID
Sbjct: 181 LLDVYNLIHDKGIFWAFDQIIETGQIPYEEIQKHVPQRKILKPIGLGPEEIGSILGQIID 240

Query: 241 TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG 300
           T+GLAPVHLVLHDSALSMAGYWVAENS  VRSLTLIDTLS PSLPLWLLELPV REV+LG
Sbjct: 241 TVGLAPVHLVLHDSALSMAGYWVAENSQSVRSLTLIDTLSNPSLPLWLLELPVAREVVLG 300

Query: 301 SNFVYSRLINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDD 360
           SNFVYS+LINFCCSKGNDAALDVEAHRVLLKGRGGRR+VVSMGKKLNHSFDIAEWGGLDD
Sbjct: 301 SNFVYSKLINFCCSKGNDAALDVEAHRVLLKGRGGRRSVVSMGKKLNHSFDIAEWGGLDD 360

Query: 361 LKSVPIQVIWSNGWSNEWSVEGRRVADVLPQAAFVKHSGGRWAQEDAANVVADSIAQFVS 420
           LKSVPIQVIWSN WSNEWS EG RVAD+LPQAAFVKHSGGRWAQEDAAN +ADSIAQFVS
Sbjct: 361 LKSVPIQVIWSNNWSNEWSAEGSRVADLLPQAAFVKHSGGRWAQEDAANAIADSIAQFVS 420

Query: 421 SLPPTVRKTVEEPEHIQKVFDELGNNDDHHHDHHHHHDHGSHGIHGAGYMDGYGLGGHAW 480
           SLP TVRK VEEPEH QKVFDELGN+D     HHHHHDHG H IHGAGYM+GYGLG  AW
Sbjct: 421 SLPLTVRKAVEEPEHTQKVFDELGNSD----QHHHHHDHGGHDIHGAGYMEGYGLGSQAW 476

BLAST of Lag0036250 vs. ExPASy Swiss-Prot
Match: Q9FZ33 (Protein AUXIN RESPONSE 4 OS=Arabidopsis thaliana OX=3702 GN=AXR4 PE=1 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 2.5e-134
Identity = 260/480 (54.17%), Postives = 333/480 (69.38%), Query Frame = 0

Query: 1   MVTITEEPDEPVPKPSKPKTNKSSSSSKPPPSTAAATPKTPSSPTPNPFTFWFYFTLIVS 60
           M  ITEE ++  PK   P  NK   S     + + +T K P   + NPF FWFYFT++VS
Sbjct: 1   MAIITEEEED--PKTLNPPKNKPKDSDF---TKSESTMKNPKPQSQNPFPFWFYFTVVVS 60

Query: 61  IITFFFISLPSLSPQ-DPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAK 120
           + T  FISL   S Q DP+SWFLSLP +LR HYS GR +KVQ++ N SPIEVF  E+G+ 
Sbjct: 61  LATIIFISLSLFSSQNDPRSWFLSLPPALRQHYSNGRTIKVQVNSNESPIEVFVAESGSI 120

Query: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKSTVEI-DESTNGVLG 180
             E VVIVHGLGLSS++F++++ SLGSKG+ ++A DLPGNGFSDKS V I  +   G + 
Sbjct: 121 HTETVVIVHGLGLSSFAFKEMIQSLGSKGIHSVAIDLPGNGFSDKSMVVIGGDREIGFVA 180

Query: 181 RLLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQK-HVPKRKILKPIGLGPEEISSILGQI 240
           R+ +VY LI EKG+FWAFDQ+IETG +PYEEI K    KR+  K I LG EE + +LGQ+
Sbjct: 181 RVKEVYGLIQEKGVFWAFDQMIETGDLPYEEIIKLQNSKRRSFKAIELGSEETARVLGQV 240

Query: 241 IDTIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVI 300
           IDT+GLAPVHLVLHDSAL +A  WV+EN   VRS+TLID+   P+LPLW+L +P +RE++
Sbjct: 241 IDTLGLAPVHLVLHDSALGLASNWVSENWQSVRSVTLIDSSISPALPLWVLNVPGIREIL 300

Query: 301 LGSNFVYSRLINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGL 360
           L  +F + +L++F CSK    + D++AHR+LLKGR GR AVV+   KLNHSFDIA+WG  
Sbjct: 301 LAFSFGFEKLVSFRCSKEMTLS-DIDAHRILLKGRNGREAVVASLNKLNHSFDIAQWGNS 360

Query: 361 DDLKSVPIQVIWSNGWSNEWSVEGRRVADVLPQAAFVKHSGGRWAQEDAANVVADSIAQF 420
           D +  +P+QVIWS+  S EWS EG+RVA  LP+A FV HSG RW QE  +  +AD I++F
Sbjct: 361 DGINGIPMQVIWSSEASKEWSDEGQRVAKALPKAKFVTHSGSRWPQESKSGELADYISEF 420

Query: 421 VSSLPPTVRKTVEE--PEHIQKVFDELGNNDDHHHDHHHHHDHGSHGIHGAGYMDGYGLG 476
           VS LP ++R+  EE  PE +QKV +E    DDH H H H H H       AGY D YGLG
Sbjct: 421 VSLLPKSIRRVAEEPIPEEVQKVLEEAKAGDDHDHHHGHGHAH-------AGYSDAYGLG 467

BLAST of Lag0036250 vs. ExPASy TrEMBL
Match: A0A6J1HWL6 (protein AUXIN RESPONSE 4 OS=Cucurbita maxima OX=3661 GN=LOC111468529 PE=4 SV=1)

HSP 1 Score: 859.8 bits (2220), Expect = 5.6e-246
Identity = 436/480 (90.83%), Postives = 448/480 (93.33%), Query Frame = 0

Query: 1   MVTITEEPDEPVPKPSKPKTNKSSSSSKPPP-STAAATPKTPSSPTPNPFTFWFYFTLIV 60
           MVTITEE DEPVPK SKPK NKSSSSSKPPP STAAAT KT  SP PNPFTFWFYFTLIV
Sbjct: 1   MVTITEESDEPVPKASKPKPNKSSSSSKPPPSSTAAATSKTLRSPNPNPFTFWFYFTLIV 60

Query: 61  SIITFFFISLPSLSPQDPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAK 120
           SIITFFFIS PSLSPQDPKSWFLSLP SLRHHYSKGR LKVQ+SPNLSPIEVFAVENGAK
Sbjct: 61  SIITFFFISFPSLSPQDPKSWFLSLPKSLRHHYSKGRLLKVQVSPNLSPIEVFAVENGAK 120

Query: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKSTVEIDESTNGVLGR 180
           GNENVV+VHGLGLSSYSFRKVLDSLGSKGVRA+AFDLPGNGFSDKST EIDES+NGV GR
Sbjct: 121 GNENVVVVHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKSTAEIDESSNGVFGR 180

Query: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEISSILGQIID 240
           LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVP+RKILKPIGLGPEEI SILGQIID
Sbjct: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPQRKILKPIGLGPEEIGSILGQIID 240

Query: 241 TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG 300
           T+GLAPVHLVLHDSALSMAGYWVAENS  VRSLTLIDTLS PSLPLWLLELPV REV+LG
Sbjct: 241 TVGLAPVHLVLHDSALSMAGYWVAENSQSVRSLTLIDTLSNPSLPLWLLELPVAREVVLG 300

Query: 301 SNFVYSRLINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDD 360
           SNFVYS+LINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDD
Sbjct: 301 SNFVYSKLINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDD 360

Query: 361 LKSVPIQVIWSNGWSNEWSVEGRRVADVLPQAAFVKHSGGRWAQEDAANVVADSIAQFVS 420
           LKSVPIQVIWSN WS+EWS EG RVAD+LPQAAFVKHSGGRWAQEDAAN VADSIAQFVS
Sbjct: 361 LKSVPIQVIWSNNWSDEWSAEGSRVADLLPQAAFVKHSGGRWAQEDAANAVADSIAQFVS 420

Query: 421 SLPPTVRKTVEEPEHIQKVFDELGNNDDHHHDHHHHHDHGSHGIHGAGYMDGYGLGGHAW 480
           SLPPTVRK VEEPEH QKVFDELGN+D     HHHHHDHGSH IHGAGYM+GYGLG  AW
Sbjct: 421 SLPPTVRKDVEEPEHTQKVFDELGNSD----QHHHHHDHGSHDIHGAGYMEGYGLGSQAW 476

BLAST of Lag0036250 vs. ExPASy TrEMBL
Match: A0A6J1EZ91 (protein AUXIN RESPONSE 4 OS=Cucurbita moschata OX=3662 GN=LOC111440912 PE=4 SV=1)

HSP 1 Score: 855.9 bits (2210), Expect = 8.0e-245
Identity = 432/480 (90.00%), Postives = 447/480 (93.12%), Query Frame = 0

Query: 1   MVTITEEPDEPVPKPSKPKTNKSSSSSKPPP-STAAATPKTPSSPTPNPFTFWFYFTLIV 60
           MVTITEE DEPVPK SKPK NKSSSSSKPPP STAAAT KT  SP PNPFTFWFYFTLIV
Sbjct: 1   MVTITEESDEPVPKASKPKPNKSSSSSKPPPSSTAAATSKTLRSPNPNPFTFWFYFTLIV 60

Query: 61  SIITFFFISLPSLSPQDPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAK 120
           SIITFFFIS+PSLSPQDPKSWFLSLP SLRHHYSKGR LKVQISPNLSPIEVFAVENGAK
Sbjct: 61  SIITFFFISVPSLSPQDPKSWFLSLPKSLRHHYSKGRLLKVQISPNLSPIEVFAVENGAK 120

Query: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKSTVEIDESTNGVLGR 180
           GNENVV+VHGLGLSSYSFRKVLDSLGSKGV A+AFDLPGNGFSDKST EIDE++NGV GR
Sbjct: 121 GNENVVVVHGLGLSSYSFRKVLDSLGSKGVHAVAFDLPGNGFSDKSTAEIDENSNGVFGR 180

Query: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEISSILGQIID 240
           LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVP+RKILKPIGLGPEEI SILGQIID
Sbjct: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPQRKILKPIGLGPEEIGSILGQIID 240

Query: 241 TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG 300
           T+GL+PVHLVLHDSALSMAGYWVAENS  VRSLTLIDTLS PSLPLWLLELPV RE++LG
Sbjct: 241 TVGLSPVHLVLHDSALSMAGYWVAENSQSVRSLTLIDTLSNPSLPLWLLELPVAREIVLG 300

Query: 301 SNFVYSRLINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDD 360
           SNFVYS+LINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDD
Sbjct: 301 SNFVYSKLINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDD 360

Query: 361 LKSVPIQVIWSNGWSNEWSVEGRRVADVLPQAAFVKHSGGRWAQEDAANVVADSIAQFVS 420
           LK VPIQVIWSN WS+EWS EG RVAD+LPQAAFVKHSGGRWAQEDAAN VADSIAQFVS
Sbjct: 361 LKRVPIQVIWSNNWSDEWSAEGSRVADLLPQAAFVKHSGGRWAQEDAANAVADSIAQFVS 420

Query: 421 SLPPTVRKTVEEPEHIQKVFDELGNNDDHHHDHHHHHDHGSHGIHGAGYMDGYGLGGHAW 480
           SLPPTVRK VEEPEH QKVFDELGN+D     HHHHHDHG H IHGAGYM+GYGLG HAW
Sbjct: 421 SLPPTVRKAVEEPEHTQKVFDELGNSD----QHHHHHDHGGHDIHGAGYMEGYGLGSHAW 476

BLAST of Lag0036250 vs. ExPASy TrEMBL
Match: A0A5D3CG86 (Protein AUXIN RESPONSE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003850 PE=3 SV=1)

HSP 1 Score: 841.6 bits (2173), Expect = 1.6e-240
Identity = 432/481 (89.81%), Postives = 448/481 (93.14%), Query Frame = 0

Query: 1   MVTITEEPDEPVPKPSKPKTNK-SSSSSKPPPSTAAATPKTPSSPTPNPFTFWFYFTLIV 60
           MVTITEEPDEPV KPS+P+ NK +SSSSKP  S+A AT KTP SPTPNPFTFWFYF LIV
Sbjct: 1   MVTITEEPDEPVRKPSEPQPNKPTSSSSKPLSSSATATHKTPPSPTPNPFTFWFYFILIV 60

Query: 61  SIITFFFISLPSLSPQDPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAK 120
           SIIT+FFISLPSLSP DPKSWFLSLPNSLRHHYSKGR LKVQIS NLSPIEVFAVENGAK
Sbjct: 61  SIITYFFISLPSLSPPDPKSWFLSLPNSLRHHYSKGRLLKVQISGNLSPIEVFAVENGAK 120

Query: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKSTVEIDESTNGVLGR 180
           GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKST EIDES+NGVLGR
Sbjct: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKSTAEIDESSNGVLGR 180

Query: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEISSILGQIID 240
           LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEI SILGQIID
Sbjct: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEIGSILGQIID 240

Query: 241 TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG 300
           TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG
Sbjct: 241 TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG 300

Query: 301 SNFVYSRLINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDD 360
           SNFVYSRLIN CCSKGNDA LDVEAHRVLLKG GGRRAVVSMGKKLN SFDIAEWGGLDD
Sbjct: 301 SNFVYSRLINLCCSKGNDAPLDVEAHRVLLKGLGGRRAVVSMGKKLNDSFDIAEWGGLDD 360

Query: 361 LKSVPIQVIWSNGWSNEWSVEGRRVADVLPQAAFVKHSGGRWAQEDAANVVADSIAQFVS 420
           LKSVP+QVIWSNGWSNEWS EGRRVA+VLPQA+FV+HSGGRWAQED A+VVADSI+QF+S
Sbjct: 361 LKSVPMQVIWSNGWSNEWSTEGRRVAEVLPQASFVEHSGGRWAQEDVADVVADSISQFIS 420

Query: 421 SLPPTVRKTVE-EPEHIQKVFDELGNNDDHHHDHHHHHDHGSHGIHGAGYMDGYGLGGHA 480
           SLPPTVRKT E  PEHI +VFD+  N+D     HHHHHDHGSHGIHGAGYMDGYGLG  A
Sbjct: 421 SLPPTVRKTEEPTPEHIHEVFDQSMNSD----HHHHHHDHGSHGIHGAGYMDGYGLGSQA 477

BLAST of Lag0036250 vs. ExPASy TrEMBL
Match: A0A1S3BP26 (protein AUXIN RESPONSE 4 OS=Cucumis melo OX=3656 GN=LOC103492173 PE=4 SV=1)

HSP 1 Score: 841.6 bits (2173), Expect = 1.6e-240
Identity = 432/481 (89.81%), Postives = 448/481 (93.14%), Query Frame = 0

Query: 1   MVTITEEPDEPVPKPSKPKTNK-SSSSSKPPPSTAAATPKTPSSPTPNPFTFWFYFTLIV 60
           MVTITEEPDEPV KPS+P+ NK +SSSSKP  S+A AT KTP SPTPNPFTFWFYF LIV
Sbjct: 1   MVTITEEPDEPVRKPSEPQPNKPTSSSSKPLSSSATATHKTPPSPTPNPFTFWFYFILIV 60

Query: 61  SIITFFFISLPSLSPQDPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAK 120
           SIIT+FFISLPSLSP DPKSWFLSLPNSLRHHYSKGR LKVQIS NLSPIEVFAVENGAK
Sbjct: 61  SIITYFFISLPSLSPPDPKSWFLSLPNSLRHHYSKGRLLKVQISGNLSPIEVFAVENGAK 120

Query: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKSTVEIDESTNGVLGR 180
           GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKST EIDES+NGVLGR
Sbjct: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKSTAEIDESSNGVLGR 180

Query: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEISSILGQIID 240
           LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEI SILGQIID
Sbjct: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEIGSILGQIID 240

Query: 241 TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG 300
           TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG
Sbjct: 241 TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG 300

Query: 301 SNFVYSRLINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDD 360
           SNFVYSRLIN CCSKGNDA LDVEAHRVLLKG GGRRAVVSMGKKLN SFDIAEWGGLDD
Sbjct: 301 SNFVYSRLINLCCSKGNDAPLDVEAHRVLLKGLGGRRAVVSMGKKLNDSFDIAEWGGLDD 360

Query: 361 LKSVPIQVIWSNGWSNEWSVEGRRVADVLPQAAFVKHSGGRWAQEDAANVVADSIAQFVS 420
           LKSVP+QVIWSNGWSNEWS EGRRVA+VLPQA+FV+HSGGRWAQED A+VVADSI+QF+S
Sbjct: 361 LKSVPMQVIWSNGWSNEWSTEGRRVAEVLPQASFVEHSGGRWAQEDVADVVADSISQFIS 420

Query: 421 SLPPTVRKTVE-EPEHIQKVFDELGNNDDHHHDHHHHHDHGSHGIHGAGYMDGYGLGGHA 480
           SLPPTVRKT E  PEHI +VFD+  N+D     HHHHHDHGSHGIHGAGYMDGYGLG  A
Sbjct: 421 SLPPTVRKTEEPTPEHIHEVFDQSMNSD----HHHHHHDHGSHGIHGAGYMDGYGLGSQA 477

BLAST of Lag0036250 vs. ExPASy TrEMBL
Match: A0A5A7U9L3 (Protein AUXIN RESPONSE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold761G00110 PE=3 SV=1)

HSP 1 Score: 836.6 bits (2160), Expect = 5.1e-239
Identity = 430/481 (89.40%), Postives = 445/481 (92.52%), Query Frame = 0

Query: 1   MVTITEEPDEPVPKPSKPKTNK-SSSSSKPPPSTAAATPKTPSSPTPNPFTFWFYFTLIV 60
           MVTITEEPDEPV KPS P+ NK +SSSSKP  S+A AT KTP SPTPNPFTFWFYF LIV
Sbjct: 1   MVTITEEPDEPVRKPSGPQPNKPTSSSSKPLSSSATATHKTPPSPTPNPFTFWFYFILIV 60

Query: 61  SIITFFFISLPSLSPQDPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAK 120
           SIIT+FFISLPSLSP DPKSWFLSLPNSLRHHYSKGR LKVQIS NLSPIEVFAVENGAK
Sbjct: 61  SIITYFFISLPSLSPPDPKSWFLSLPNSLRHHYSKGRLLKVQISGNLSPIEVFAVENGAK 120

Query: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKSTVEIDESTNGVLGR 180
           GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKST EIDES+NGVLGR
Sbjct: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKSTAEIDESSNGVLGR 180

Query: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEISSILGQIID 240
           LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEI SILGQIID
Sbjct: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEIGSILGQIID 240

Query: 241 TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG 300
           TIGL PVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG
Sbjct: 241 TIGLTPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG 300

Query: 301 SNFVYSRLINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDD 360
           SNFVYSRLI  CCSKGNDA LDVEAHRVLLKG GGRRAVVSMGKKLN SFDIAEWGGLDD
Sbjct: 301 SNFVYSRLIKLCCSKGNDAPLDVEAHRVLLKGLGGRRAVVSMGKKLNDSFDIAEWGGLDD 360

Query: 361 LKSVPIQVIWSNGWSNEWSVEGRRVADVLPQAAFVKHSGGRWAQEDAANVVADSIAQFVS 420
           LKSVP+QVIWSNGWSNEWS EGRRVA+VLPQA+FV+HSGGRWAQED A+VVADSI+QF+S
Sbjct: 361 LKSVPMQVIWSNGWSNEWSTEGRRVAEVLPQASFVEHSGGRWAQEDVADVVADSISQFIS 420

Query: 421 SLPPTVRKTVE-EPEHIQKVFDELGNNDDHHHDHHHHHDHGSHGIHGAGYMDGYGLGGHA 480
           SLPPTVRKT E  PEHI +VFD+  N+D     HHHHHDHGSHGIHGAGYMDGYGLG  A
Sbjct: 421 SLPPTVRKTEEPTPEHIHEVFDQSMNSD----HHHHHHDHGSHGIHGAGYMDGYGLGSQA 477

BLAST of Lag0036250 vs. TAIR 10
Match: AT1G54990.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 480.3 bits (1235), Expect = 1.8e-135
Identity = 260/480 (54.17%), Postives = 333/480 (69.38%), Query Frame = 0

Query: 1   MVTITEEPDEPVPKPSKPKTNKSSSSSKPPPSTAAATPKTPSSPTPNPFTFWFYFTLIVS 60
           M  ITEE ++  PK   P  NK   S     + + +T K P   + NPF FWFYFT++VS
Sbjct: 1   MAIITEEEED--PKTLNPPKNKPKDSDF---TKSESTMKNPKPQSQNPFPFWFYFTVVVS 60

Query: 61  IITFFFISLPSLSPQ-DPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAK 120
           + T  FISL   S Q DP+SWFLSLP +LR HYS GR +KVQ++ N SPIEVF  E+G+ 
Sbjct: 61  LATIIFISLSLFSSQNDPRSWFLSLPPALRQHYSNGRTIKVQVNSNESPIEVFVAESGSI 120

Query: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKSTVEI-DESTNGVLG 180
             E VVIVHGLGLSS++F++++ SLGSKG+ ++A DLPGNGFSDKS V I  +   G + 
Sbjct: 121 HTETVVIVHGLGLSSFAFKEMIQSLGSKGIHSVAIDLPGNGFSDKSMVVIGGDREIGFVA 180

Query: 181 RLLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQK-HVPKRKILKPIGLGPEEISSILGQI 240
           R+ +VY LI EKG+FWAFDQ+IETG +PYEEI K    KR+  K I LG EE + +LGQ+
Sbjct: 181 RVKEVYGLIQEKGVFWAFDQMIETGDLPYEEIIKLQNSKRRSFKAIELGSEETARVLGQV 240

Query: 241 IDTIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVI 300
           IDT+GLAPVHLVLHDSAL +A  WV+EN   VRS+TLID+   P+LPLW+L +P +RE++
Sbjct: 241 IDTLGLAPVHLVLHDSALGLASNWVSENWQSVRSVTLIDSSISPALPLWVLNVPGIREIL 300

Query: 301 LGSNFVYSRLINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGL 360
           L  +F + +L++F CSK    + D++AHR+LLKGR GR AVV+   KLNHSFDIA+WG  
Sbjct: 301 LAFSFGFEKLVSFRCSKEMTLS-DIDAHRILLKGRNGREAVVASLNKLNHSFDIAQWGNS 360

Query: 361 DDLKSVPIQVIWSNGWSNEWSVEGRRVADVLPQAAFVKHSGGRWAQEDAANVVADSIAQF 420
           D +  +P+QVIWS+  S EWS EG+RVA  LP+A FV HSG RW QE  +  +AD I++F
Sbjct: 361 DGINGIPMQVIWSSEASKEWSDEGQRVAKALPKAKFVTHSGSRWPQESKSGELADYISEF 420

Query: 421 VSSLPPTVRKTVEE--PEHIQKVFDELGNNDDHHHDHHHHHDHGSHGIHGAGYMDGYGLG 476
           VS LP ++R+  EE  PE +QKV +E    DDH H H H H H       AGY D YGLG
Sbjct: 421 VSLLPKSIRRVAEEPIPEEVQKVLEEAKAGDDHDHHHGHGHAH-------AGYSDAYGLG 467

BLAST of Lag0036250 vs. TAIR 10
Match: AT4G12830.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 50.1 bits (118), Expect = 5.9e-06
Identity = 25/53 (47.17%), Postives = 36/53 (67.92%), Query Frame = 0

Query: 112 FAVENGAKGNENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDK 165
           F+VE+G+  +  V+++HG    +YS+RK +  L SK  RA+AFD  G GFSDK
Sbjct: 124 FSVESGSVDSPPVILIHGFPSQAYSYRKTIPVL-SKNYRAIAFDWLGFGFSDK 175

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038880139.11.2e-25091.89protein AUXIN RESPONSE 4 [Benincasa hispida][more]
XP_022969547.11.2e-24590.83protein AUXIN RESPONSE 4 [Cucurbita maxima][more]
KAG6588477.11.7e-24489.79Protein AUXIN RESPONSE 4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022933511.11.7e-24490.00protein AUXIN RESPONSE 4 [Cucurbita moschata][more]
XP_023530238.17.0e-24389.38protein AUXIN RESPONSE 4 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9FZ332.5e-13454.17Protein AUXIN RESPONSE 4 OS=Arabidopsis thaliana OX=3702 GN=AXR4 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HWL65.6e-24690.83protein AUXIN RESPONSE 4 OS=Cucurbita maxima OX=3661 GN=LOC111468529 PE=4 SV=1[more]
A0A6J1EZ918.0e-24590.00protein AUXIN RESPONSE 4 OS=Cucurbita moschata OX=3662 GN=LOC111440912 PE=4 SV=1[more]
A0A5D3CG861.6e-24089.81Protein AUXIN RESPONSE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3BP261.6e-24089.81protein AUXIN RESPONSE 4 OS=Cucumis melo OX=3656 GN=LOC103492173 PE=4 SV=1[more]
A0A5A7U9L35.1e-23989.40Protein AUXIN RESPONSE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
Match NameE-valueIdentityDescription
AT1G54990.11.8e-13554.17alpha/beta-Hydrolases superfamily protein [more]
AT4G12830.15.9e-0647.17alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 124..281
e-value: 3.1E-8
score: 33.6
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 105..420
e-value: 3.0E-24
score: 88.3
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 108..419
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 442..462
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..43
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 17..33
NoneNo IPR availablePANTHERPTHR43329:SF81PROTEIN AUXIN RESPONSE 4coord: 37..459
NoneNo IPR availablePANTHERPTHR43329EPOXIDE HYDROLASEcoord: 37..459

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0036250.1Lag0036250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009926 auxin polar transport
biological_process GO:0007049 cell cycle
biological_process GO:0051301 cell division
biological_process GO:0009733 response to auxin
biological_process GO:0009612 response to mechanical stimulus
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016787 hydrolase activity