Lag0035717 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0035717
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionNon-specific serine/threonine protein kinase
Locationchr3: 28432246 .. 28434917 (-)
RNA-Seq ExpressionLag0035717
SyntenyLag0035717
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGATACCGATTCATTCAATGCTTCCTCCACTCTTCTTCACCATCCAGATTTCTCTCACTATGTTGAAATTGATCCCACTCGCAGATATGGAAGAGTACTCCTCTTTCTCTCTCTCTCTCTTCATCCGCTTTCATTCTCATTCTCATTCTATTCTTTTCTTTCTTGCAGTACGATCAAATCCTCGGCAAAGGAGCTTCCAAGACAGTGTATCAACATACAATTCACCCTCTCCCTCCTCTCCTTTGCATTTCTCTAATTCCTATCCATTTTCCGATTTTGTTACATTTTCCCTTCCTTTTCAGAATTTCATCTCTTCTGCCTGTTTCATTGTTGTTCCTTTGCTTCTCTTTCTCGCCTAATAAGGGCGTTTCATTTTTTTAATCTGATTTAGTTTTTGGGAATGGGAAATTCGATTGAGGGGTTTTTTTTTTTGTTTTTTTTTTTTTTGTGTGTTGCAGTTACAGAGCATTTGATGAGTTTGAAGGGATTGAAGTTGCTTGGAACCAGGTCAAACTCTGTAACTTCCTTCAATGCCCTGAAGATCTTGAGAGGCTATACTCTGAGATTCATCTTTTAAAGACATTGAAACATAAGAACATCATGAAATTCTATACTTCTTGGGTCGACACTGCAAATAGGAACATCAACTTTGTAACTGAAATGTTCACTTCTGGGACTTTGAGGCAGTAAGAATGATTTTCAGCATTCATCTTATCTTACACCCTTCACATAGCCTTCTCCCTTTTTATGATTTCTGCTAAATTTCTGGTTGGTTGGTTCTGTTCTGACAGGTATAGGCTAAAGCATAGGAGAGTTAATGTTAGAGCAGTGAAGCGATGGTGTAGACAGATCTTGAGGGGTCTTCTCTATTTACACAGCCAAAACCCCCCTGTGATCCACAGAGATCTCAAGTGTGACAACATTTTTGTTAATGGTAACCAAGGAGAAATTAAAATTGGAGATCTTGGTCTTGCTGCTATCCTCCGGAAATCGCATGCTGATCATTGCGTCGGTATGTATGTTTATCTTCTTCAAATCCTGTGAAATTTTGGCAATAGATTCTACTTCTGGAAAGGATGATCTTGGCTTTCTGATGTTAAATGCCCTTTCAGTGAATCTTTTTCCTTGAATTGTTGGTGAGTTTTGTGAGAGAAGTAAGTCCTCTCGTTAGATGTTTCTTACTGTTGTTATTACATTACAGGGACACCAGAGTTCATGGCTCCTGAAGTATATGAAGAGGCATATAATGAATTGGTAGACATTTATTCTTTTGGGATGTGTGTTTTGGAGATGGTTACATTTGAATATCCATATAGTGAATGTAATCATCCTGTTCAAATCTATAAGAAAGTAATTTCTGTAAGATCTCCCTCTCTCAATGGGCATGTATTCTTCTTTAATCTTTTATGTGCGGGAAGAAAAAATCGATTCCTTAACATGCTTTCTAGTTTCTACTTGTAGGGGAAAAAACCAGATGCCTTGTATAAAGTGAAGGATCCGAGCATGCGGCAATTTGTCGAGAAATGCTTGGCACCAGTCTCTTGTAGACTTTCGGCAAGGGAGCTTTTGAGTGACCCTTTTCTCCAAATTGATGATTGTGAAAGCAAGTCAAAACTTTCAGATTTTCGGGAAGAACTGGATGGTATTGCTTCTACTACAGCACAACCTTTCCTTGAACAAGAGAAAAGTTTGAGCTCGATTAATTACAGTTTGGAGGCCTCAGATGAATGGAGGTATCTTACAGTTCCGACTGTACCCGATGGTATCGAACTTTTTGAGGACGAGGATAATGACCAATTGGAAAATCTTGATAACAGTATTAAGGGGAAGATAAGAGAAGATGGCAGCATTGTTTTAAGACTTAGGATTGCAGAGAAAGAAGGTAAGACACATTTATTTTCCTCCTTTCGTATTTCGTCATGGAATGCTTGCCTTCTATAGTTACACAAACATTAACTCGAACGATCGGGTAAATGTGCAGGGCATATTCGAAACATATATTTCCCATTTGACACCAAGAATGATACGGCATTGACAGTTGCCACTGAAATGATAGCAGAACTTGATATTACCGACCAAGATGTGATCAAGATAGCTGAAAAAATTGATGGGGAAATCGCTTCCTTGGTACCGGAGTGGAAGCCGGGGCCTGGCATAGATGAAACACCTCGCATCTCCTATGATGGTGGTTGTCAAAGCTACGATGCTTCTAACCATCCATCTGAGAATCATTTGATAGGGAATAAGCGAAATGGAGCTAAACTCTATCAGATTTTAAACTGCAGCACAGATGAATGTGCTACAGCAGACAGACATTTCGAGCAGGTCTCCCTCGAAGCAGATCAGCCTCCACAGCCTCTAGTAGATAGCACACCAAAAGTGTCAAGCCAGCATTATCAGCAAAACTCAGTCCATGAAAACCAAGCTCTTGGTTCTCGTAGTTTTAGGCGAAGTCATTCTGATCAAGATCAACATTACAAGAAAACAGGCCAATCAATCGCTGTTGGGGATAACAAAGAAAAGTTACTTGATAGCAAAGCCACTGTCATTGCAAGAACTTCACTCAGAAGCTTACTGGGTTCCCGATCGCTGTCTACGGTTTCGTCCTATTGTGAAGATAAATTTGCATCTCAAATCCATTGGGAAATTAGATGGCTTTGGAATTGA

mRNA sequence

ATGGACGATACCGATTCATTCAATGCTTCCTCCACTCTTCTTCACCATCCAGATTTCTCTCACTATGTTGAAATTGATCCCACTCGCAGATATGGAAGATACGATCAAATCCTCGGCAAAGGAGCTTCCAAGACAGTTTACAGAGCATTTGATGAGTTTGAAGGGATTGAAGTTGCTTGGAACCAGGTCAAACTCTGTAACTTCCTTCAATGCCCTGAAGATCTTGAGAGGCTATACTCTGAGATTCATCTTTTAAAGACATTGAAACATAAGAACATCATGAAATTCTATACTTCTTGGGTCGACACTGCAAATAGGAACATCAACTTTGTAACTGAAATGTTCACTTCTGGGACTTTGAGGCAGTATAGGCTAAAGCATAGGAGAGTTAATGTTAGAGCAGTGAAGCGATGGTGTAGACAGATCTTGAGGGGTCTTCTCTATTTACACAGCCAAAACCCCCCTGTGATCCACAGAGATCTCAAGTGTGACAACATTTTTGTTAATGGTAACCAAGGAGAAATTAAAATTGGAGATCTTGGTCTTGCTGCTATCCTCCGGAAATCGCATGCTGATCATTGCGTCGGGACACCAGAGTTCATGGCTCCTGAAGTATATGAAGAGGCATATAATGAATTGGTAGACATTTATTCTTTTGGGATGTGTGTTTTGGAGATGGTTACATTTGAATATCCATATAGTGAATGTAATCATCCTGTTCAAATCTATAAGAAAGTAATTTCTGGGAAAAAACCAGATGCCTTGTATAAAGTGAAGGATCCGAGCATGCGGCAATTTGTCGAGAAATGCTTGGCACCAGTCTCTTGTAGACTTTCGGCAAGGGAGCTTTTGAGTGACCCTTTTCTCCAAATTGATGATTGTGAAAGCAAGTCAAAACTTTCAGATTTTCGGGAAGAACTGGATGGTATTGCTTCTACTACAGCACAACCTTTCCTTGAACAAGAGAAAAGTTTGAGCTCGATTAATTACAGTTTGGAGGCCTCAGATGAATGGAGGTATCTTACAGTTCCGACTGTACCCGATGGTATCGAACTTTTTGAGGACGAGGATAATGACCAATTGGAAAATCTTGATAACAGTATTAAGGGGAAGATAAGAGAAGATGGCAGCATTGTTTTAAGACTTAGGATTGCAGAGAAAGAAGGGCATATTCGAAACATATATTTCCCATTTGACACCAAGAATGATACGGCATTGACAGTTGCCACTGAAATGATAGCAGAACTTGATATTACCGACCAAGATGTGATCAAGATAGCTGAAAAAATTGATGGGGAAATCGCTTCCTTGGTACCGGAGTGGAAGCCGGGGCCTGGCATAGATGAAACACCTCGCATCTCCTATGATGGTGGTTGTCAAAGCTACGATGCTTCTAACCATCCATCTGAGAATCATTTGATAGGGAATAAGCGAAATGGAGCTAAACTCTATCAGATTTTAAACTGCAGCACAGATGAATGTGCTACAGCAGACAGACATTTCGAGCAGGTCTCCCTCGAAGCAGATCAGCCTCCACAGCCTCTAGTAGATAGCACACCAAAAGTGTCAAGCCAGCATTATCAGCAAAACTCAGTCCATGAAAACCAAGCTCTTGGTTCTCGTAGTTTTAGGCGAAGTCATTCTGATCAAGATCAACATTACAAGAAAACAGGCCAATCAATCGCTGTTGGGGATAACAAAGAAAAGTTACTTGATAGCAAAGCCACTGTCATTGCAAGAACTTCACTCAGAAGCTTACTGGGTTCCCGATCGCTGTCTACGGTTTCGTCCTATTGTGAAGATAAATTTGCATCTCAAATCCATTGGGAAATTAGATGGCTTTGGAATTGA

Coding sequence (CDS)

ATGGACGATACCGATTCATTCAATGCTTCCTCCACTCTTCTTCACCATCCAGATTTCTCTCACTATGTTGAAATTGATCCCACTCGCAGATATGGAAGATACGATCAAATCCTCGGCAAAGGAGCTTCCAAGACAGTTTACAGAGCATTTGATGAGTTTGAAGGGATTGAAGTTGCTTGGAACCAGGTCAAACTCTGTAACTTCCTTCAATGCCCTGAAGATCTTGAGAGGCTATACTCTGAGATTCATCTTTTAAAGACATTGAAACATAAGAACATCATGAAATTCTATACTTCTTGGGTCGACACTGCAAATAGGAACATCAACTTTGTAACTGAAATGTTCACTTCTGGGACTTTGAGGCAGTATAGGCTAAAGCATAGGAGAGTTAATGTTAGAGCAGTGAAGCGATGGTGTAGACAGATCTTGAGGGGTCTTCTCTATTTACACAGCCAAAACCCCCCTGTGATCCACAGAGATCTCAAGTGTGACAACATTTTTGTTAATGGTAACCAAGGAGAAATTAAAATTGGAGATCTTGGTCTTGCTGCTATCCTCCGGAAATCGCATGCTGATCATTGCGTCGGGACACCAGAGTTCATGGCTCCTGAAGTATATGAAGAGGCATATAATGAATTGGTAGACATTTATTCTTTTGGGATGTGTGTTTTGGAGATGGTTACATTTGAATATCCATATAGTGAATGTAATCATCCTGTTCAAATCTATAAGAAAGTAATTTCTGGGAAAAAACCAGATGCCTTGTATAAAGTGAAGGATCCGAGCATGCGGCAATTTGTCGAGAAATGCTTGGCACCAGTCTCTTGTAGACTTTCGGCAAGGGAGCTTTTGAGTGACCCTTTTCTCCAAATTGATGATTGTGAAAGCAAGTCAAAACTTTCAGATTTTCGGGAAGAACTGGATGGTATTGCTTCTACTACAGCACAACCTTTCCTTGAACAAGAGAAAAGTTTGAGCTCGATTAATTACAGTTTGGAGGCCTCAGATGAATGGAGGTATCTTACAGTTCCGACTGTACCCGATGGTATCGAACTTTTTGAGGACGAGGATAATGACCAATTGGAAAATCTTGATAACAGTATTAAGGGGAAGATAAGAGAAGATGGCAGCATTGTTTTAAGACTTAGGATTGCAGAGAAAGAAGGGCATATTCGAAACATATATTTCCCATTTGACACCAAGAATGATACGGCATTGACAGTTGCCACTGAAATGATAGCAGAACTTGATATTACCGACCAAGATGTGATCAAGATAGCTGAAAAAATTGATGGGGAAATCGCTTCCTTGGTACCGGAGTGGAAGCCGGGGCCTGGCATAGATGAAACACCTCGCATCTCCTATGATGGTGGTTGTCAAAGCTACGATGCTTCTAACCATCCATCTGAGAATCATTTGATAGGGAATAAGCGAAATGGAGCTAAACTCTATCAGATTTTAAACTGCAGCACAGATGAATGTGCTACAGCAGACAGACATTTCGAGCAGGTCTCCCTCGAAGCAGATCAGCCTCCACAGCCTCTAGTAGATAGCACACCAAAAGTGTCAAGCCAGCATTATCAGCAAAACTCAGTCCATGAAAACCAAGCTCTTGGTTCTCGTAGTTTTAGGCGAAGTCATTCTGATCAAGATCAACATTACAAGAAAACAGGCCAATCAATCGCTGTTGGGGATAACAAAGAAAAGTTACTTGATAGCAAAGCCACTGTCATTGCAAGAACTTCACTCAGAAGCTTACTGGGTTCCCGATCGCTGTCTACGGTTTCGTCCTATTGTGAAGATAAATTTGCATCTCAAATCCATTGGGAAATTAGATGGCTTTGGAATTGA

Protein sequence

MDDTDSFNASSTLLHHPDFSHYVEIDPTRRYGRYDQILGKGASKTVYRAFDEFEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTLRQYRLKHRRVNVRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKLSDFREELDGIASTTAQPFLEQEKSLSSINYSLEASDEWRYLTVPTVPDGIELFEDEDNDQLENLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRNGAKLYQILNCSTDECATADRHFEQVSLEADQPPQPLVDSTPKVSSQHYQQNSVHENQALGSRSFRRSHSDQDQHYKKTGQSIAVGDNKEKLLDSKATVIARTSLRSLLGSRSLSTVSSYCEDKFASQIHWEIRWLWN
Homology
BLAST of Lag0035717 vs. NCBI nr
Match: KAG6588353.1 (putative serine/threonine-protein kinase WNK1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022205.1 putative serine/threonine-protein kinase WNK1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1031.6 bits (2666), Expect = 2.8e-297
Identity = 524/617 (84.93%), Postives = 556/617 (90.11%), Query Frame = 0

Query: 1   MDDTDSFNASSTLLHHPDFSHYVEIDPTRRYGRYDQILGKGASKTVYRAFDEFEGIEVAW 60
           M+  DS    S LL  PDFS YVEIDPT RYGRY++ILGKGASKTVYRAFDE+EGIEVAW
Sbjct: 1   METIDSITGFSNLLQPPDFSQYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120

Query: 121 RQYRLKHRRVNVRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQYRLKHRRVN+RAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNI VNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNILVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCR SARELLSDPFLQI+DCESK +L
Sbjct: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRPSARELLSDPFLQINDCESKLQL 300

Query: 301 SDFREELDGIASTTAQPFLEQEKSLSSINYSLEASDEWRYLTVPTVPDGIELFEDEDNDQ 360
           S    ELDGIA TTA PFLE++ S SSINY LEASDEWR  +V T PDG ELFE++D+DQ
Sbjct: 301 SGCPRELDGIAFTTAHPFLERQGSFSSINYHLEASDEWRCRSVQTEPDGFELFENDDDDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIVLRLRIA+KEG IRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRNG 480
           QDVIKIAEKIDGEIASLVPEWK GPGIDETPRISYDGGCQS+DA+NH  ++ LIGN +NG
Sbjct: 421 QDVIKIAEKIDGEIASLVPEWKSGPGIDETPRISYDGGCQSHDAANHSCDHSLIGNNQNG 480

Query: 481 AKLYQILNCSTDECATADRHFEQVSLEADQPPQPLVDSTPKVSSQHYQQNSV-HENQALG 540
            KLYQILN ST E A+    F+Q SL+AD+PP+P+V+S PKVSSQHYQ NSV  E+ ALG
Sbjct: 481 EKLYQILNLSTVERASVHELFKQDSLKADRPPEPVVESPPKVSSQHYQPNSVLLEDPALG 540

Query: 541 SRSFRRSHSDQDQHYKKTGQSIAVGDNKEKLLDSKATVIARTSLRSLLGSRSLSTVSSYC 600
           S SFR SHS  +Q++KK GQSI VGD+KEK+LD  A  IARTSLRSLLGSRSLSTV SY 
Sbjct: 541 SHSFRHSHS--NQNFKKIGQSIIVGDDKEKILDKNA--IARTSLRSLLGSRSLSTVPSYR 600

Query: 601 EDKFASQIHWEIRWLWN 617
           EDKFASQIH EIRWLWN
Sbjct: 601 EDKFASQIHREIRWLWN 613

BLAST of Lag0035717 vs. NCBI nr
Match: XP_023001906.1 (probable serine/threonine-protein kinase WNK9 [Cucurbita maxima])

HSP 1 Score: 1031.2 bits (2665), Expect = 3.7e-297
Identity = 524/617 (84.93%), Postives = 555/617 (89.95%), Query Frame = 0

Query: 1   MDDTDSFNASSTLLHHPDFSHYVEIDPTRRYGRYDQILGKGASKTVYRAFDEFEGIEVAW 60
           M+  DS    S LL  PDFS YVEIDPT RYGRY++ILGKGASKTVYRAFDE+EGIEVAW
Sbjct: 1   METIDSITGFSNLLQPPDFSQYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLK+LKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKSLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120

Query: 121 RQYRLKHRRVNVRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQYRLKHRRVN+RAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNI VNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNILVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCR SARELLSDPFLQIDDCESK +L
Sbjct: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRPSARELLSDPFLQIDDCESKLQL 300

Query: 301 SDFREELDGIASTTAQPFLEQEKSLSSINYSLEASDEWRYLTVPTVPDGIELFEDEDNDQ 360
           S    EL+GIA TTA PFLE++ S SSINY LEASDEWR  +V T PDG ELFE++D+DQ
Sbjct: 301 SGCPRELNGIAFTTAHPFLERQGSFSSINYCLEASDEWRCRSVQTEPDGFELFENDDDDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIVLRLRIA+KEG IRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRNG 480
           QDVIKIAEKIDGEIASLVPEWK GPGIDETPRISYDGGCQS+DA+NH  ++ LIGN +NG
Sbjct: 421 QDVIKIAEKIDGEIASLVPEWKSGPGIDETPRISYDGGCQSHDAANHSCDHSLIGNNQNG 480

Query: 481 AKLYQILNCSTDECATADRHFEQVSLEADQPPQPLVDSTPKVSSQHYQQNSV-HENQALG 540
            KLYQILN ST E A+    F+Q SL+AD PP+P+V+S PKVSSQHYQ NSV  EN ALG
Sbjct: 481 EKLYQILNLSTVERASVHELFKQDSLKADPPPEPVVESPPKVSSQHYQPNSVLLENPALG 540

Query: 541 SRSFRRSHSDQDQHYKKTGQSIAVGDNKEKLLDSKATVIARTSLRSLLGSRSLSTVSSYC 600
           S SFR SHS  +Q++KK GQSI VGD+KEK+LD  A  IARTSLRSLLGSRSLSTV SY 
Sbjct: 541 SHSFRHSHS--NQNFKKIGQSIIVGDDKEKILDKNA--IARTSLRSLLGSRSLSTVPSYR 600

Query: 601 EDKFASQIHWEIRWLWN 617
           EDKFASQIH EIRWLWN
Sbjct: 601 EDKFASQIHREIRWLWN 613

BLAST of Lag0035717 vs. NCBI nr
Match: XP_038880702.1 (probable serine/threonine-protein kinase WNK9 isoform X1 [Benincasa hispida])

HSP 1 Score: 1030.8 bits (2664), Expect = 4.8e-297
Identity = 523/619 (84.49%), Postives = 554/619 (89.50%), Query Frame = 0

Query: 1   MDDTDSFNASSTLLHHPDFSHYVEIDPTRRYGRYDQILGKGASKTVYRAFDEFEGIEVAW 60
           MD TDS +  S L+  PDFS YVEIDPT RYGRY++ILGKGASK VYRAFDE+EGIEVAW
Sbjct: 1   MDKTDSISGFSDLVQLPDFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTAN+NINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL 120

Query: 121 RQYRLKHRRVNVRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQ+RLKHRRVN+RAVKRWCRQILRGL YLHSQ+PPVIHRDLKCDNIFVNGNQGEIKIGDL
Sbjct: 121 RQFRLKHRRVNIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSEC+HPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECSHPV 240

Query: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKVISGKKP ALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQ+DD ESKSK+
Sbjct: 241 QIYKKVISGKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVDDGESKSKI 300

Query: 301 SDFREELDGIASTTAQPFLEQEKSLSSINYSLEASDEWRYLTVPTVPDGIELFEDEDNDQ 360
           S+ R ELD  AST A PFLEQEK  SSI+YS E SDEWR  TV    DGIELFED+DND 
Sbjct: 301 SNSRGELDDFASTIAHPFLEQEKRFSSISYSFETSDEWRCHTVQKEADGIELFEDDDNDI 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIVLRLRIA+KEG IRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRNG 480
           QDVIKIAEKIDGEIASLVP+WKPGPGIDETPRISYDGG +SY+ SN PS+N LI NK NG
Sbjct: 421 QDVIKIAEKIDGEIASLVPDWKPGPGIDETPRISYDGGSRSYNDSNQPSDNSLIENKGNG 480

Query: 481 AKLYQILNCSTDECATADRHFE--QVSLEADQPPQPLVDSTPKVSSQHYQQNS-VHENQA 540
           AKLYQILNCS D  A    HFE  Q SL+ADQP Q +V+S PKVSSQH Q NS +HENQA
Sbjct: 481 AKLYQILNCSIDGRALVQGHFEQGQFSLKADQPTQSVVESRPKVSSQHNQSNSALHENQA 540

Query: 541 LGSRSFRRSHSDQDQHYKKTGQSIAVGDNKEKLLDSKATVIARTSLRSLLGSRSLSTVSS 600
           L S SFR+ HS   ++YKKT QS+ VG +KEK+ D+KATVI RTSLRSLLGSRSLSTV S
Sbjct: 541 LSSHSFRQRHS--GENYKKTDQSVTVGYSKEKVPDTKATVIDRTSLRSLLGSRSLSTVPS 600

Query: 601 YCEDKFASQIHWEIRWLWN 617
           YCEDKFASQIHWEIRWLWN
Sbjct: 601 YCEDKFASQIHWEIRWLWN 617

BLAST of Lag0035717 vs. NCBI nr
Match: XP_022933886.1 (probable serine/threonine-protein kinase WNK9 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1030.0 bits (2662), Expect = 8.3e-297
Identity = 523/617 (84.76%), Postives = 555/617 (89.95%), Query Frame = 0

Query: 1   MDDTDSFNASSTLLHHPDFSHYVEIDPTRRYGRYDQILGKGASKTVYRAFDEFEGIEVAW 60
           M+  DS    S LL  PDFS YVEIDPT RYGRY++ILGKGASKTVYRAFDE+EGIEVAW
Sbjct: 1   METIDSITGFSNLLQPPDFSQYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120

Query: 121 RQYRLKHRRVNVRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQYRLKHRRVN+RAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNI VNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNILVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKV+SGKKPDALYKVKDPSMRQFVEKCLAPVSCR SARELLSDPFLQIDDCESK +L
Sbjct: 241 QIYKKVVSGKKPDALYKVKDPSMRQFVEKCLAPVSCRPSARELLSDPFLQIDDCESKLQL 300

Query: 301 SDFREELDGIASTTAQPFLEQEKSLSSINYSLEASDEWRYLTVPTVPDGIELFEDEDNDQ 360
           S    ELDGIA TTA PFLE++ S SSINY LEASDEWR  +V T PDG ELFE++D+DQ
Sbjct: 301 SGCPRELDGIAFTTAHPFLERQGSFSSINYRLEASDEWRCHSVQTEPDGFELFENDDDDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIVLRLRIA+KEG IRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRNG 480
           QDVIKIAEKIDGEIASLVPEWK GPGIDETPRISYDG CQS+DA+NH  ++ LIGN +NG
Sbjct: 421 QDVIKIAEKIDGEIASLVPEWKSGPGIDETPRISYDGDCQSHDAANHSCDHSLIGNNQNG 480

Query: 481 AKLYQILNCSTDECATADRHFEQVSLEADQPPQPLVDSTPKVSSQHYQQNSV-HENQALG 540
            KLYQILN ST E A+    F+Q SL+AD+PP+P+V+S PKVSSQHYQ NSV  E+ ALG
Sbjct: 481 EKLYQILNLSTVERASVHELFKQDSLKADRPPEPVVESPPKVSSQHYQPNSVLLEDPALG 540

Query: 541 SRSFRRSHSDQDQHYKKTGQSIAVGDNKEKLLDSKATVIARTSLRSLLGSRSLSTVSSYC 600
           S SFR SHS  +Q++KK GQSI VGD+KEK+LD  A  IARTSLRSLLGSRSLSTV SY 
Sbjct: 541 SHSFRHSHS--NQNFKKIGQSIIVGDDKEKILDKNA--IARTSLRSLLGSRSLSTVPSYR 600

Query: 601 EDKFASQIHWEIRWLWN 617
           EDKFASQIH EIRWLWN
Sbjct: 601 EDKFASQIHREIRWLWN 613

BLAST of Lag0035717 vs. NCBI nr
Match: XP_023529957.1 (probable serine/threonine-protein kinase WNK9 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1029.6 bits (2661), Expect = 1.1e-296
Identity = 524/617 (84.93%), Postives = 555/617 (89.95%), Query Frame = 0

Query: 1   MDDTDSFNASSTLLHHPDFSHYVEIDPTRRYGRYDQILGKGASKTVYRAFDEFEGIEVAW 60
           M+  DS    S LL  PDFS YVEIDPT RYGRY++ILGKGASKTVYRAFDE+EGIEVAW
Sbjct: 1   METIDSITGFSNLLQPPDFSQYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120

Query: 121 RQYRLKHRRVNVRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQYRLKHRRVN+RAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNI VNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNILVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCR SARELLSDPFLQIDDCESK +L
Sbjct: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRPSARELLSDPFLQIDDCESKLQL 300

Query: 301 SDFREELDGIASTTAQPFLEQEKSLSSINYSLEASDEWRYLTVPTVPDGIELFEDEDNDQ 360
           S    ELD IA TTA PFLE++ S SSINY LEASDEWR  +V T PDG ELFE++D+DQ
Sbjct: 301 SGCPRELDCIAFTTAHPFLERQGSFSSINYRLEASDEWRCHSVQTEPDGFELFENDDDDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIVLRLRIA+KEG IRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRNG 480
           QDVIKIAEKIDGEIASLVPEWK GPGIDETPRISYDGGCQS+DA+NH  ++ LIGN +NG
Sbjct: 421 QDVIKIAEKIDGEIASLVPEWKSGPGIDETPRISYDGGCQSHDAANHSCDHSLIGNNQNG 480

Query: 481 AKLYQILNCSTDECATADRHFEQVSLEADQPPQPLVDSTPKVSSQHYQQNSV-HENQALG 540
            KLYQILN ST E A+    F+Q SL+AD+PP+P+V+S PKVSSQHYQ NSV  E+ ALG
Sbjct: 481 EKLYQILNLSTVERASVHELFKQDSLKADRPPEPVVESPPKVSSQHYQPNSVLLEDPALG 540

Query: 541 SRSFRRSHSDQDQHYKKTGQSIAVGDNKEKLLDSKATVIARTSLRSLLGSRSLSTVSSYC 600
           S SFR SHS  +Q++KK GQSI VGD+KEK+LD  A  IARTSLRSLLGSRSLSTV SY 
Sbjct: 541 SHSFRHSHS--NQNFKKIGQSIIVGDDKEKILDKNA--IARTSLRSLLGSRSLSTVPSYR 600

Query: 601 EDKFASQIHWEIRWLWN 617
           EDKFASQIH EIRWLWN
Sbjct: 601 EDKFASQIHREIRWLWN 613

BLAST of Lag0035717 vs. ExPASy Swiss-Prot
Match: A2YMV6 (Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. indica OX=39946 GN=WNK1 PE=2 SV=2)

HSP 1 Score: 638.6 bits (1646), Expect = 7.0e-182
Identity = 333/548 (60.77%), Postives = 391/548 (71.35%), Query Frame = 0

Query: 18  DFSHYVEIDPTRRYGRYDQILGKGASKTVYRAFDEFEGIEVAWNQVKLCNFLQCPEDLER 77
           D   Y E+DPT RYGRY+ +LGKGASKTVYRAFDE++G+EVAWNQVKL +FLQ PEDLER
Sbjct: 13  DLPEYAEVDPTGRYGRYNDVLGKGASKTVYRAFDEYQGMEVAWNQVKLHDFLQSPEDLER 72

Query: 78  LYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTLRQYRLKHRRVNVRAVKR 137
           LY EIHLLKTLKH+NIMKFYTSWVD + RNINF+TEMFTSGTLRQYR KH RVN+ AVK 
Sbjct: 73  LYCEIHLLKTLKHRNIMKFYTSWVDVSRRNINFITEMFTSGTLRQYRQKHMRVNIWAVKH 132

Query: 138 WCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGT 197
           WCRQIL GLLYLHS +PP+IHRDLKCDNIFVNGNQGE+KIGDLGLAAILRKSHA HCVGT
Sbjct: 133 WCRQILSGLLYLHSHDPPIIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAVHCVGT 192

Query: 198 PEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYK 257
           PEFMAPEVYEE YNELVDIYSFGMCVLEMVTFEYPYSEC HPVQIYKKVISG KP+ALYK
Sbjct: 193 PEFMAPEVYEEEYNELVDIYSFGMCVLEMVTFEYPYSECTHPVQIYKKVISGTKPEALYK 252

Query: 258 VKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKLSDFREELDGIASTTAQP 317
           VKDP +RQFVEKCLA  S RLSARELL DPFLQ+DD        D+      + +   QP
Sbjct: 253 VKDPMVRQFVEKCLATASRRLSARELLKDPFLQVDDLVFCPGDGDY-----SLMNYLRQP 312

Query: 318 FLEQEKS---------LSSINYSLEASDEWRYLTVPTVPDGIELFEDEDNDQLENLDNSI 377
           +LE   S           SI+      D W         DGI+LF   +++ L N+D +I
Sbjct: 313 YLEHAYSNVSMMSNGLSESIDEDTPTEDRWDCEDDDIKADGIDLFNGHEDEPLGNVDITI 372

Query: 378 KGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAE 437
           KG+  EDGSI LRLRIA+ +GH+RNIYFPFD + DTAL+VATEM+AELDITD +V +IAE
Sbjct: 373 KGRKSEDGSIFLRLRIADNDGHVRNIYFPFDIEADTALSVATEMVAELDITDHEVTRIAE 432

Query: 438 KIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRNGAKLYQILN 497
            IDGE+++LVP+W+PGPGI+E+   +Y   C S              N  +   LY  ++
Sbjct: 433 MIDGEVSALVPDWRPGPGIEESQDTTYCHNCGS--------------NVSSCGSLYAYMS 492

Query: 498 CSTDECATADRH--FEQVSLEADQPPQPLVDSTPKVSSQHYQQNSVHENQALGSRSF--- 551
            +   C  A+ H  FE+++ +A+     L DS         Q   V + +A+ S  F   
Sbjct: 493 SAARGCQCAELHGRFEEITFQANGEQTDLQDSGGSSDDGGGQTQHVKDQEAVHSNGFVQM 541

BLAST of Lag0035717 vs. ExPASy Swiss-Prot
Match: Q0D598 (Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. japonica OX=39947 GN=WNK1 PE=2 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 7.0e-182
Identity = 333/548 (60.77%), Postives = 391/548 (71.35%), Query Frame = 0

Query: 18  DFSHYVEIDPTRRYGRYDQILGKGASKTVYRAFDEFEGIEVAWNQVKLCNFLQCPEDLER 77
           D   Y E+DPT RYGRY+ +LGKGASKTVYRAFDE++G+EVAWNQVKL +FLQ PEDLER
Sbjct: 13  DLPEYAEVDPTGRYGRYNDVLGKGASKTVYRAFDEYQGMEVAWNQVKLHDFLQSPEDLER 72

Query: 78  LYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTLRQYRLKHRRVNVRAVKR 137
           LY EIHLLKTLKH+NIMKFYTSWVD + RNINF+TEMFTSGTLRQYR KH RVN+ AVK 
Sbjct: 73  LYCEIHLLKTLKHRNIMKFYTSWVDVSRRNINFITEMFTSGTLRQYRQKHMRVNIWAVKH 132

Query: 138 WCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGT 197
           WCRQIL GLLYLHS +PP+IHRDLKCDNIFVNGNQGE+KIGDLGLAAILRKSHA HCVGT
Sbjct: 133 WCRQILSGLLYLHSHDPPIIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAVHCVGT 192

Query: 198 PEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYK 257
           PEFMAPEVYEE YNELVDIYSFGMCVLEMVTFEYPYSEC HPVQIYKKVISG KP+ALYK
Sbjct: 193 PEFMAPEVYEEEYNELVDIYSFGMCVLEMVTFEYPYSECTHPVQIYKKVISGTKPEALYK 252

Query: 258 VKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKLSDFREELDGIASTTAQP 317
           VKDP +RQFVEKCLA  S RLSARELL DPFLQ+DD        D+      + +   QP
Sbjct: 253 VKDPMVRQFVEKCLATASRRLSARELLKDPFLQVDDLVFCPGDGDY-----SLMNYLRQP 312

Query: 318 FLEQEKS---------LSSINYSLEASDEWRYLTVPTVPDGIELFEDEDNDQLENLDNSI 377
           +LE   S           SI+      D W         DGI+LF   +++ L N+D +I
Sbjct: 313 YLEHAYSNVSMMSNGLSESIDEDTPTEDRWDCEDDDIKADGIDLFNGHEDEPLGNVDITI 372

Query: 378 KGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAE 437
           KG+  EDGSI LRLRIA+ +GH+RNIYFPFD + DTAL+VATEM+AELDITD +V +IAE
Sbjct: 373 KGRKSEDGSIFLRLRIADNDGHVRNIYFPFDIEADTALSVATEMVAELDITDHEVTRIAE 432

Query: 438 KIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRNGAKLYQILN 497
            IDGE+++LVP+W+PGPGI+E+   +Y   C S              N  +   LY  ++
Sbjct: 433 MIDGEVSALVPDWRPGPGIEESQDTTYCHNCGS--------------NVSSCGSLYAYMS 492

Query: 498 CSTDECATADRH--FEQVSLEADQPPQPLVDSTPKVSSQHYQQNSVHENQALGSRSF--- 551
            +   C  A+ H  FE+++ +A+     L DS         Q   V + +A+ S  F   
Sbjct: 493 SAARGCQCAELHGRFEEITFQANGEQTDLQDSGGSSDDGGGQTQHVKDQEAVHSNGFVQM 541

BLAST of Lag0035717 vs. ExPASy Swiss-Prot
Match: Q9CAV6 (Serine/threonine-protein kinase WNK1 OS=Arabidopsis thaliana OX=3702 GN=WNK1 PE=1 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 2.3e-180
Identity = 349/641 (54.45%), Postives = 426/641 (66.46%), Query Frame = 0

Query: 17  PDFSHYVEIDPTRRYGRYDQILGKGASKTVYRAFDEFEGIEVAWNQVKLCNFLQCPEDLE 76
           PD+S +VE+DPT RYGRY+++LGKGASKTVYRAFDE+EGIEVAWNQVKL +FLQ PEDLE
Sbjct: 9   PDYSEFVEVDPTGRYGRYNEVLGKGASKTVYRAFDEYEGIEVAWNQVKLYDFLQSPEDLE 68

Query: 77  RLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTLRQYRLKHRRVNVRAVK 136
           RLY EIHLLKTLKHKNIMKFYTSWVDTANRNINFVTE+FTSGTLRQYRL+H+RVN+RA+K
Sbjct: 69  RLYCEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTELFTSGTLRQYRLRHKRVNIRAMK 128

Query: 137 RWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVG 196
            WCRQILRGL YLHS +PPVIHRDLKCDNIFVNGNQGE+KIGDLGLAAILRKSHA HCVG
Sbjct: 129 HWCRQILRGLHYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAAHCVG 188

Query: 197 TPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALY 256
           TPEFMAPEVYEEAYNELVDIYSFGMC+LEMVTF+YPYSEC HP QIYKKV+SGKKPDALY
Sbjct: 189 TPEFMAPEVYEEAYNELVDIYSFGMCILEMVTFDYPYSECTHPAQIYKKVMSGKKPDALY 248

Query: 257 KVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKLSDFREELDGIASTTAQ 316
           KVKDP ++ F+EKCLA VS R+SARELL DPFL+IDD E   +  D     D +     Q
Sbjct: 249 KVKDPEVKCFIEKCLATVSLRVSARELLDDPFLRIDDGEFDLRSVDME---DSVGPLYRQ 308

Query: 317 P-----FLEQEKSLSSIN------------------YS------LEASDEWRYLTVPT-V 376
           P     +     + SS+N                  YS       E  + W Y    T  
Sbjct: 309 PHHLPDYYNYPSNSSSLNRQYSNGNYPSNSSSLNRQYSNGYNSHHEYQNGWAYNPAETEE 368

Query: 377 PDGIELFEDEDNDQLE------NLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDT 436
             GIELFE  +ND  E      N+D +IKGK R+DG + LRLRIA+KEG +RNIYFPFD 
Sbjct: 369 THGIELFESRNNDDQEEEKKSGNVDITIKGKRRDDGGLFLRLRIADKEGRVRNIYFPFDI 428

Query: 437 KNDTALTVATEMIAELDITDQDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQ 496
           + DTAL+VATEM+AELD+ D  V KIA  IDGEI+SLVP W+PGP  +E    +      
Sbjct: 429 ETDTALSVATEMVAELDMDDHGVTKIANMIDGEISSLVPSWRPGPEFEECLAAAAAANAA 488

Query: 497 SY---DASNHPSENHLIGNKRNGAKLYQILNCSTDECATADRHFEQVSLEADQPPQPLVD 556
           S      SN  S   ++   R       ++ C  + C      FE++++           
Sbjct: 489 SICNNCVSNRTSMGSVMDFLRTNPGA-NVIQCCRNGCGETHGRFEEITIR---------- 548

Query: 557 STPKVSSQHYQQNSVHENQALGSRSFRRSHSDQDQHYKKTGQSIAVGDNKEKLLDSKATV 615
            T     + ++     E++ L S     +HS++++      +   + ++ E +   +   
Sbjct: 549 ETEVRLRELWKLQQQQESRELSSIDSGHNHSEEEE------EEEVLYEDPENMFSCE--- 608

BLAST of Lag0035717 vs. ExPASy Swiss-Prot
Match: Q2V338 (Probable serine/threonine-protein kinase WNK9 OS=Arabidopsis thaliana OX=3702 GN=WNK9 PE=2 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 2.6e-160
Identity = 289/437 (66.13%), Postives = 339/437 (77.57%), Query Frame = 0

Query: 18  DFSHYVEIDPTRRYGRYDQILGKGASKTVYRAFDEFEGIEVAWNQVKLCNFLQCPEDLER 77
           D+S YVE+DPT RYGRY+++LGKG+SKTVYR FDE++GIEVAWNQVKL +FLQ P++LER
Sbjct: 11  DYSEYVEVDPTGRYGRYNEVLGKGSSKTVYRGFDEYQGIEVAWNQVKLYDFLQSPQELER 70

Query: 78  LYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTLRQYRLKHRRVNVRAVKR 137
           LY EIHLLKTLKHK+IMKFY SWVDT NRNINFVTEMFTSGTLRQYRLKH+RVN+RAVK 
Sbjct: 71  LYCEIHLLKTLKHKSIMKFYASWVDTDNRNINFVTEMFTSGTLRQYRLKHKRVNIRAVKN 130

Query: 138 WCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGT 197
           WCRQILRGL YLH+ +PPVIHRDLKCDNIF+NGNQGE+KIGDLGLAA L+ SHA HCVGT
Sbjct: 131 WCRQILRGLNYLHTHDPPVIHRDLKCDNIFINGNQGEVKIGDLGLAACLQHSHAAHCVGT 190

Query: 198 PEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYK 257
           PEFMAPEVY+E YN+LVDIYSFGMCVLEMVTF+YPYSEC+HP QIYK+VISGKKPD L K
Sbjct: 191 PEFMAPEVYKEEYNQLVDIYSFGMCVLEMVTFDYPYSECSHPAQIYKRVISGKKPDGLDK 250

Query: 258 VKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKLSDFR--EELDGIASTTA 317
           VKDP +R F+EKCLA VS RLSA ELL D FL ID+       SD R  E   G+     
Sbjct: 251 VKDPEVRGFIEKCLATVSLRLSACELLDDHFLCIDE-------SDMRRVESEKGLIDEAG 310

Query: 318 QPFLEQE--KSLSSINYSLEASDEWRYLTVPTVP----DGIELFEDEDNDQLE----NLD 377
            P          S+  YSL   ++W Y    TV     D +E   D+D ++ +    ++D
Sbjct: 311 TPLRHSYHIPHYSNGYYSLYNQNQWDYNGDETVESHEIDLLEFQNDDDEEEEDKRFGSVD 370

Query: 378 NSIKGKIREDG-SIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITDQDVI 437
            SIKGK R++G  + LRL+   KEG +RNIYFPFD + DTA++VA EM+ EL++ D+DV 
Sbjct: 371 ISIKGKRRDNGDGLFLRLKTVNKEGCVRNIYFPFDIETDTAISVAREMVEELEMDDRDVT 430

Query: 438 KIAEKIDGEIASLVPEW 442
           KIA  IDGEIASLVP W
Sbjct: 431 KIANMIDGEIASLVPNW 440

BLAST of Lag0035717 vs. ExPASy Swiss-Prot
Match: Q8S8Y9 (Serine/threonine-protein kinase WNK2 OS=Arabidopsis thaliana OX=3702 GN=WNK2 PE=1 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 9.9e-160
Identity = 283/426 (66.43%), Postives = 333/426 (78.17%), Query Frame = 0

Query: 18  DFSHYVEIDPTRRYGRYDQILGKGASKTVYRAFDEFEGIEVAWNQVKLCNFLQCPEDLER 77
           D S +VEIDP+ RYGRYD+ILGKGASKTVYRAFDE+EGIEVAWNQVKL NF + PE+LE+
Sbjct: 10  DCSVFVEIDPSGRYGRYDEILGKGASKTVYRAFDEYEGIEVAWNQVKLRNFTRNPEELEK 69

Query: 78  LYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTLRQYRLKHRRVNVRAVKR 137
            + EIHLLKTL H+NIMKFYTSWVDT N +INFVTE+FTSGTLRQYRL+HRRVN+RAVK+
Sbjct: 70  FFREIHLLKTLNHQNIMKFYTSWVDTNNLSINFVTELFTSGTLRQYRLRHRRVNIRAVKQ 129

Query: 138 WCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGT 197
           WC+QIL+GLLYLHS++PP+IHRDLKCDNIF+NGNQGE+KIGDLGLAAILRKSHA  CVGT
Sbjct: 130 WCKQILKGLLYLHSRSPPIIHRDLKCDNIFINGNQGEVKIGDLGLAAILRKSHAVRCVGT 189

Query: 198 PEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYK 257
           PEFMAPEVY+E YNELVD+Y+FGMCVLEMVTF+YPYSEC HP QIYKKV SGKKP+A Y 
Sbjct: 190 PEFMAPEVYDEEYNELVDVYAFGMCVLEMVTFDYPYSECTHPAQIYKKVTSGKKPEAFYL 249

Query: 258 VKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCES-KSKLSDFREELDGIASTTAQ 317
           VKDP +R+FVEKCLA V+CRL+A ELL DPFLQ D+ +    +  D+    D        
Sbjct: 250 VKDPEVREFVEKCLANVTCRLTALELLQDPFLQDDNMDGFVMRPIDYYNGYDETGVFLRH 309

Query: 318 PFLEQEKSLSSINYSLEASDEWRYLTVPTVPDGIELFEDEDNDQLENLDNSIKGKIREDG 377
           P ++          S                  I+LF ++D D   ++D SIKGK   D 
Sbjct: 310 PLIDDPLYHDQFESSQICE--------------IDLFANDDED---HVDISIKGKRNGDD 369

Query: 378 SIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEIAS 437
            I LRLRI++ EG IRNIYFPF+T  DTA +VA EM++ELDIT+QDV KIAE ID EIA+
Sbjct: 370 GIFLRLRISDAEGRIRNIYFPFETAIDTAWSVAVEMVSELDITNQDVAKIAEMIDAEIAA 418

Query: 438 LVPEWK 443
           LVP+WK
Sbjct: 430 LVPDWK 418

BLAST of Lag0035717 vs. ExPASy TrEMBL
Match: A0A6J1KJY6 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111495945 PE=4 SV=1)

HSP 1 Score: 1031.2 bits (2665), Expect = 1.8e-297
Identity = 524/617 (84.93%), Postives = 555/617 (89.95%), Query Frame = 0

Query: 1   MDDTDSFNASSTLLHHPDFSHYVEIDPTRRYGRYDQILGKGASKTVYRAFDEFEGIEVAW 60
           M+  DS    S LL  PDFS YVEIDPT RYGRY++ILGKGASKTVYRAFDE+EGIEVAW
Sbjct: 1   METIDSITGFSNLLQPPDFSQYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLK+LKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKSLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120

Query: 121 RQYRLKHRRVNVRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQYRLKHRRVN+RAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNI VNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNILVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCR SARELLSDPFLQIDDCESK +L
Sbjct: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRPSARELLSDPFLQIDDCESKLQL 300

Query: 301 SDFREELDGIASTTAQPFLEQEKSLSSINYSLEASDEWRYLTVPTVPDGIELFEDEDNDQ 360
           S    EL+GIA TTA PFLE++ S SSINY LEASDEWR  +V T PDG ELFE++D+DQ
Sbjct: 301 SGCPRELNGIAFTTAHPFLERQGSFSSINYCLEASDEWRCRSVQTEPDGFELFENDDDDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIVLRLRIA+KEG IRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRNG 480
           QDVIKIAEKIDGEIASLVPEWK GPGIDETPRISYDGGCQS+DA+NH  ++ LIGN +NG
Sbjct: 421 QDVIKIAEKIDGEIASLVPEWKSGPGIDETPRISYDGGCQSHDAANHSCDHSLIGNNQNG 480

Query: 481 AKLYQILNCSTDECATADRHFEQVSLEADQPPQPLVDSTPKVSSQHYQQNSV-HENQALG 540
            KLYQILN ST E A+    F+Q SL+AD PP+P+V+S PKVSSQHYQ NSV  EN ALG
Sbjct: 481 EKLYQILNLSTVERASVHELFKQDSLKADPPPEPVVESPPKVSSQHYQPNSVLLENPALG 540

Query: 541 SRSFRRSHSDQDQHYKKTGQSIAVGDNKEKLLDSKATVIARTSLRSLLGSRSLSTVSSYC 600
           S SFR SHS  +Q++KK GQSI VGD+KEK+LD  A  IARTSLRSLLGSRSLSTV SY 
Sbjct: 541 SHSFRHSHS--NQNFKKIGQSIIVGDDKEKILDKNA--IARTSLRSLLGSRSLSTVPSYR 600

Query: 601 EDKFASQIHWEIRWLWN 617
           EDKFASQIH EIRWLWN
Sbjct: 601 EDKFASQIHREIRWLWN 613

BLAST of Lag0035717 vs. ExPASy TrEMBL
Match: A0A6J1F099 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111441166 PE=4 SV=1)

HSP 1 Score: 1030.0 bits (2662), Expect = 4.0e-297
Identity = 523/617 (84.76%), Postives = 555/617 (89.95%), Query Frame = 0

Query: 1   MDDTDSFNASSTLLHHPDFSHYVEIDPTRRYGRYDQILGKGASKTVYRAFDEFEGIEVAW 60
           M+  DS    S LL  PDFS YVEIDPT RYGRY++ILGKGASKTVYRAFDE+EGIEVAW
Sbjct: 1   METIDSITGFSNLLQPPDFSQYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120

Query: 121 RQYRLKHRRVNVRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQYRLKHRRVN+RAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNI VNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNILVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKV+SGKKPDALYKVKDPSMRQFVEKCLAPVSCR SARELLSDPFLQIDDCESK +L
Sbjct: 241 QIYKKVVSGKKPDALYKVKDPSMRQFVEKCLAPVSCRPSARELLSDPFLQIDDCESKLQL 300

Query: 301 SDFREELDGIASTTAQPFLEQEKSLSSINYSLEASDEWRYLTVPTVPDGIELFEDEDNDQ 360
           S    ELDGIA TTA PFLE++ S SSINY LEASDEWR  +V T PDG ELFE++D+DQ
Sbjct: 301 SGCPRELDGIAFTTAHPFLERQGSFSSINYRLEASDEWRCHSVQTEPDGFELFENDDDDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIVLRLRIA+KEG IRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRNG 480
           QDVIKIAEKIDGEIASLVPEWK GPGIDETPRISYDG CQS+DA+NH  ++ LIGN +NG
Sbjct: 421 QDVIKIAEKIDGEIASLVPEWKSGPGIDETPRISYDGDCQSHDAANHSCDHSLIGNNQNG 480

Query: 481 AKLYQILNCSTDECATADRHFEQVSLEADQPPQPLVDSTPKVSSQHYQQNSV-HENQALG 540
            KLYQILN ST E A+    F+Q SL+AD+PP+P+V+S PKVSSQHYQ NSV  E+ ALG
Sbjct: 481 EKLYQILNLSTVERASVHELFKQDSLKADRPPEPVVESPPKVSSQHYQPNSVLLEDPALG 540

Query: 541 SRSFRRSHSDQDQHYKKTGQSIAVGDNKEKLLDSKATVIARTSLRSLLGSRSLSTVSSYC 600
           S SFR SHS  +Q++KK GQSI VGD+KEK+LD  A  IARTSLRSLLGSRSLSTV SY 
Sbjct: 541 SHSFRHSHS--NQNFKKIGQSIIVGDDKEKILDKNA--IARTSLRSLLGSRSLSTVPSYR 600

Query: 601 EDKFASQIHWEIRWLWN 617
           EDKFASQIH EIRWLWN
Sbjct: 601 EDKFASQIHREIRWLWN 613

BLAST of Lag0035717 vs. ExPASy TrEMBL
Match: A0A1S3CI45 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103501021 PE=4 SV=1)

HSP 1 Score: 1029.6 bits (2661), Expect = 5.2e-297
Identity = 527/619 (85.14%), Postives = 554/619 (89.50%), Query Frame = 0

Query: 1   MDDTDSFNASSTLLHHPDFSHYVEIDPTRRYGRYDQILGKGASKTVYRAFDEFEGIEVAW 60
           MD TDS  A S LL  PDFSHYVEIDPT RYGRY++ILGKGASKTVYRAFDE+EGIEVAW
Sbjct: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTAN+NINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL 120

Query: 121 RQYRLKHRRVNVRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQYRLKHRR N+RAVKRWCRQILRGL YLHSQ+PPVIHRDLKCDNIFVNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRANIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAIL+KSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILQKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFL+ID CESK K+
Sbjct: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLEIDICESKLKI 300

Query: 301 SDFREELDGIASTTAQPFLEQEKSLSSINYSLEASDEWRYLTVPTVPDGIELFEDEDNDQ 360
           SD R ELD  AST  +PFLE+EK  SSI+YSLEASDEWRY +V    DGIELFED DNDQ
Sbjct: 301 SDSRRELDDFASTIVRPFLEREKRFSSISYSLEASDEWRYRSVQKERDGIELFEDNDNDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LENLD +IKGKIREDGSIVLRLRI +KEG IRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LENLDKNIKGKIREDGSIVLRLRITDKEGLIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRNG 480
           QDVIKIAEKIDGEI+SLVPEWKPGPGIDETPRISYDGG QSY+ASN PS+N LI NK NG
Sbjct: 421 QDVIKIAEKIDGEISSLVPEWKPGPGIDETPRISYDGGSQSYNASNQPSDNILIENKGNG 480

Query: 481 AKLYQILNCSTDECATADRHF--EQVSLEADQPPQPLVDSTPKVSSQHYQQNSV-HENQA 540
            KLYQILN STD  A A +HF  EQ SL+AD+P QP+V S P VSSQHYQ +SV  ENQA
Sbjct: 481 IKLYQILNLSTDGHALAHKHFEQEQFSLKADRPTQPVVGSIPNVSSQHYQPDSVLPENQA 540

Query: 541 LGSRSFRRSHSDQDQHYKKTGQSIAVGDNKEKLLDSKATVIARTSLRSLLGSRSLSTVSS 600
           L SRSFR+ H   D +YKKT QS+AVG  KEKL D KATVI  TS RSLLGSRSLS VSS
Sbjct: 541 LSSRSFRQRH--YDDNYKKTDQSLAVG-YKEKLPDKKATVI-DTSQRSLLGSRSLSRVSS 600

Query: 601 YCEDKFASQIHWEIRWLWN 617
           YCEDKF+SQIHWEIRWLWN
Sbjct: 601 YCEDKFSSQIHWEIRWLWN 615

BLAST of Lag0035717 vs. ExPASy TrEMBL
Match: A0A0A0M0J2 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_1G695390 PE=4 SV=1)

HSP 1 Score: 1018.5 bits (2632), Expect = 1.2e-293
Identity = 522/619 (84.33%), Postives = 548/619 (88.53%), Query Frame = 0

Query: 1   MDDTDSFNASSTLLHHPDFSHYVEIDPTRRYGRYDQILGKGASKTVYRAFDEFEGIEVAW 60
           MD TDS  A S LL  PDFSHYVEIDPT RYGRYD+ILGKGASKTVYRAFDE+EGIEVAW
Sbjct: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRYDEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTAN+NINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL 120

Query: 121 RQYRLKHRRVNVRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQYRLKHRR N+RAVKRWCRQILRGL YLHSQ+PPVIHRDLKCDNIFVNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRANIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKVISGKKP ALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFL+ID CESK K+
Sbjct: 241 QIYKKVISGKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLEIDGCESKLKI 300

Query: 301 SDFREELDGIASTTAQPFLEQEKSLSSINYSLEASDEWRYLTVPTVPDGIELFEDEDNDQ 360
           SD R ELD  AST  +PFLE+EK  SSI+YSLE SDEWRY +V   PDGIELFED DNDQ
Sbjct: 301 SDSRRELDDFASTIVRPFLEREKRFSSISYSLEGSDEWRYRSVQKEPDGIELFEDNDNDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           L +LDN+IKGKIREDGSIVLRLRI +KEG IRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LVSLDNNIKGKIREDGSIVLRLRITDKEGLIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRNG 480
           QDVIKIAEKIDGEI+SLVPEWKPGPGIDETPRISYDGG QSY+A N PS+N LI NK NG
Sbjct: 421 QDVIKIAEKIDGEISSLVPEWKPGPGIDETPRISYDGGSQSYNACNQPSDNILIENKGNG 480

Query: 481 AKLYQILNCSTDECATADRHF--EQVSLEADQPPQPLVDSTPKVSSQHYQQNSV-HENQA 540
            KLYQILN STD  A A  HF  EQ SL+AD+P Q      P VSSQHYQ +SV +ENQA
Sbjct: 481 IKLYQILNLSTDGHALAHEHFEQEQFSLKADRPTQ------PNVSSQHYQPDSVLNENQA 540

Query: 541 LGSRSFRRSHSDQDQHYKKTGQSIAVGDNKEKLLDSKATVIARTSLRSLLGSRSLSTVSS 600
           L S SFR+ HS  D +YKK  QS+ VG NKEKL  +KATVI  TS RSLLGSRSLSTVSS
Sbjct: 541 LSSHSFRQRHS--DDNYKKIDQSLTVGYNKEKLPVNKATVI-DTSQRSLLGSRSLSTVSS 600

Query: 601 YCEDKFASQIHWEIRWLWN 617
           YCEDKF+SQIHWEIRWLWN
Sbjct: 601 YCEDKFSSQIHWEIRWLWN 610

BLAST of Lag0035717 vs. ExPASy TrEMBL
Match: A0A6J1DKV6 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111020998 PE=4 SV=1)

HSP 1 Score: 1008.4 bits (2606), Expect = 1.2e-290
Identity = 513/617 (83.14%), Postives = 546/617 (88.49%), Query Frame = 0

Query: 1   MDDTDSFNASSTLLHHPDFSHYVEIDPTRRYGRYDQILGKGASKTVYRAFDEFEGIEVAW 60
           M+  +S +  S  LHHPD S YVEIDP+ RYGRY++ILGKGASKTVYRAFDE+EGIEVAW
Sbjct: 1   MEKDESIDGFSN-LHHPDSSRYVEIDPSGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120
           NQVKLCN LQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSG+L
Sbjct: 61  NQVKLCNLLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGSL 120

Query: 121 RQYRLKHRRVNVRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQ+RLKHRRVN RAVKRWCRQILR LLYLHSQ+PPVIHRDLKCDNIFVNGNQGEIKIGDL
Sbjct: 121 RQFRLKHRRVNNRAVKRWCRQILRALLYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240
           GLAAILRKSHAD CVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV
Sbjct: 181 GLAAILRKSHADRCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKL 300
           QIYKKV SG+KP ALYKVKDPSMRQFVEKCLAPV  RLSARELLSDPFLQIDDCESKSK+
Sbjct: 241 QIYKKVTSGEKPAALYKVKDPSMRQFVEKCLAPVPYRLSARELLSDPFLQIDDCESKSKI 300

Query: 301 SDFREELDGIASTTAQPFLEQEKSLSSINYSLEASDEWRYLTVPTVPDGIELFEDEDNDQ 360
           SD + ELD IAST  QPFLE+E S SSINYSL+ SDEWR   V T PDG ELFED+D+DQ
Sbjct: 301 SDCQGELDRIASTIVQPFLEREISFSSINYSLDTSDEWRCRIVETEPDGTELFEDDDDDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LEN+DNSI GKIREDGSIVLRLRIA+KEG IRNIYFPFDTK DTALTVATEMIAELDITD
Sbjct: 361 LENVDNSITGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKMDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQSYDASNHPSENHLIGNKRNG 480
           QDV KIAEKIDGEIASLVPEWKPGPGIDETPRISYDG  QSYD SNHPS NHLI   +NG
Sbjct: 421 QDVTKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGDLQSYDTSNHPSYNHLIEKMQNG 480

Query: 481 AKLYQILNCSTDECATADRHFEQVSLEADQPPQPLVDSTPKVSSQHYQQNSV-HENQALG 540
           AK  QILNCS DE A+A RHFEQVS + DQP QP+V+  PKVS+QHYQ NSV HE QAL 
Sbjct: 481 AKNSQILNCSADERASAPRHFEQVSHKEDQPTQPVVERIPKVSNQHYQLNSVLHEGQALS 540

Query: 541 SRSFRRSHSDQDQHYKKTGQSIAVGDNKEKLLDSKATVIARTSLRSLLGSRSLSTVSSYC 600
           S SFR+SHSDQ+   KKTGQ I  G++ EK+  SKAT IA++S+RSLLGSRSLS V SY 
Sbjct: 541 SHSFRQSHSDQNN--KKTGQLITGGNDIEKIQVSKATAIAKSSIRSLLGSRSLSRVPSYR 600

Query: 601 EDKFASQIHWEIRWLWN 617
           EDKFASQIHWEIRWLWN
Sbjct: 601 EDKFASQIHWEIRWLWN 614

BLAST of Lag0035717 vs. TAIR 10
Match: AT3G04910.1 (with no lysine (K) kinase 1 )

HSP 1 Score: 633.6 bits (1633), Expect = 1.6e-181
Identity = 349/641 (54.45%), Postives = 426/641 (66.46%), Query Frame = 0

Query: 17  PDFSHYVEIDPTRRYGRYDQILGKGASKTVYRAFDEFEGIEVAWNQVKLCNFLQCPEDLE 76
           PD+S +VE+DPT RYGRY+++LGKGASKTVYRAFDE+EGIEVAWNQVKL +FLQ PEDLE
Sbjct: 9   PDYSEFVEVDPTGRYGRYNEVLGKGASKTVYRAFDEYEGIEVAWNQVKLYDFLQSPEDLE 68

Query: 77  RLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTLRQYRLKHRRVNVRAVK 136
           RLY EIHLLKTLKHKNIMKFYTSWVDTANRNINFVTE+FTSGTLRQYRL+H+RVN+RA+K
Sbjct: 69  RLYCEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTELFTSGTLRQYRLRHKRVNIRAMK 128

Query: 137 RWCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVG 196
            WCRQILRGL YLHS +PPVIHRDLKCDNIFVNGNQGE+KIGDLGLAAILRKSHA HCVG
Sbjct: 129 HWCRQILRGLHYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAAHCVG 188

Query: 197 TPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALY 256
           TPEFMAPEVYEEAYNELVDIYSFGMC+LEMVTF+YPYSEC HP QIYKKV+SGKKPDALY
Sbjct: 189 TPEFMAPEVYEEAYNELVDIYSFGMCILEMVTFDYPYSECTHPAQIYKKVMSGKKPDALY 248

Query: 257 KVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKLSDFREELDGIASTTAQ 316
           KVKDP ++ F+EKCLA VS R+SARELL DPFL+IDD E   +  D     D +     Q
Sbjct: 249 KVKDPEVKCFIEKCLATVSLRVSARELLDDPFLRIDDGEFDLRSVDME---DSVGPLYRQ 308

Query: 317 P-----FLEQEKSLSSIN------------------YS------LEASDEWRYLTVPT-V 376
           P     +     + SS+N                  YS       E  + W Y    T  
Sbjct: 309 PHHLPDYYNYPSNSSSLNRQYSNGNYPSNSSSLNRQYSNGYNSHHEYQNGWAYNPAETEE 368

Query: 377 PDGIELFEDEDNDQLE------NLDNSIKGKIREDGSIVLRLRIAEKEGHIRNIYFPFDT 436
             GIELFE  +ND  E      N+D +IKGK R+DG + LRLRIA+KEG +RNIYFPFD 
Sbjct: 369 THGIELFESRNNDDQEEEKKSGNVDITIKGKRRDDGGLFLRLRIADKEGRVRNIYFPFDI 428

Query: 437 KNDTALTVATEMIAELDITDQDVIKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGGCQ 496
           + DTAL+VATEM+AELD+ D  V KIA  IDGEI+SLVP W+PGP  +E    +      
Sbjct: 429 ETDTALSVATEMVAELDMDDHGVTKIANMIDGEISSLVPSWRPGPEFEECLAAAAAANAA 488

Query: 497 SY---DASNHPSENHLIGNKRNGAKLYQILNCSTDECATADRHFEQVSLEADQPPQPLVD 556
           S      SN  S   ++   R       ++ C  + C      FE++++           
Sbjct: 489 SICNNCVSNRTSMGSVMDFLRTNPGA-NVIQCCRNGCGETHGRFEEITIR---------- 548

Query: 557 STPKVSSQHYQQNSVHENQALGSRSFRRSHSDQDQHYKKTGQSIAVGDNKEKLLDSKATV 615
            T     + ++     E++ L S     +HS++++      +   + ++ E +   +   
Sbjct: 549 ETEVRLRELWKLQQQQESRELSSIDSGHNHSEEEE------EEEVLYEDPENMFSCE--- 608

BLAST of Lag0035717 vs. TAIR 10
Match: AT3G04910.2 (with no lysine (K) kinase 1 )

HSP 1 Score: 583.2 bits (1502), Expect = 2.5e-166
Identity = 327/612 (53.43%), Postives = 399/612 (65.20%), Query Frame = 0

Query: 46  VYRAFDEFEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTAN 105
           +YRAFDE+EGIEVAWNQVKL +FLQ PEDLERLY EIHLLKTLKHKNIMKFYTSWVDTAN
Sbjct: 15  LYRAFDEYEGIEVAWNQVKLYDFLQSPEDLERLYCEIHLLKTLKHKNIMKFYTSWVDTAN 74

Query: 106 RNINFVTEMFTSGTLRQYRLKHRRVNVRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDN 165
           RNINFVTE+FTSGTLRQYRL+H+RVN+RA+K WCRQILRGL YLHS +PPVIHRDLKCDN
Sbjct: 75  RNINFVTELFTSGTLRQYRLRHKRVNIRAMKHWCRQILRGLHYLHSHDPPVIHRDLKCDN 134

Query: 166 IFVNGNQGEIKIGDLGLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLE 225
           IFVNGNQGE+KIGDLGLAAILRKSHA HCVGTPEFMAPEVYEEAYNELVDIYSFGMC+LE
Sbjct: 135 IFVNGNQGEVKIGDLGLAAILRKSHAAHCVGTPEFMAPEVYEEAYNELVDIYSFGMCILE 194

Query: 226 MVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLS 285
           MVTF+YPYSEC HP QIYKKV+SGKKPDALYKVKDP ++ F+EKCLA VS R+SARELL 
Sbjct: 195 MVTFDYPYSECTHPAQIYKKVMSGKKPDALYKVKDPEVKCFIEKCLATVSLRVSARELLD 254

Query: 286 DPFLQIDDCESKSKLSDFREELDGIASTTAQP-----FLEQEKSLSSIN----------- 345
           DPFL+IDD E   +  D     D +     QP     +     + SS+N           
Sbjct: 255 DPFLRIDDGEFDLRSVDME---DSVGPLYRQPHHLPDYYNYPSNSSSLNRQYSNGNYPSN 314

Query: 346 -------YS------LEASDEWRYLTVPT-VPDGIELFEDEDNDQLE------NLDNSIK 405
                  YS       E  + W Y    T    GIELFE  +ND  E      N+D +IK
Sbjct: 315 SSSLNRQYSNGYNSHHEYQNGWAYNPAETEETHGIELFESRNNDDQEEEKKSGNVDITIK 374

Query: 406 GKIREDGSIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEK 465
           GK R+DG + LRLRIA+KEG +RNIYFPFD + DTAL+VATEM+AELD+ D  V KIA  
Sbjct: 375 GKRRDDGGLFLRLRIADKEGRVRNIYFPFDIETDTALSVATEMVAELDMDDHGVTKIANM 434

Query: 466 IDGEIASLVPEWKPGPGIDETPRISYDGGCQSY---DASNHPSENHLIGNKRNGAKLYQI 525
           IDGEI+SLVP W+PGP  +E    +      S      SN  S   ++   R       +
Sbjct: 435 IDGEISSLVPSWRPGPEFEECLAAAAAANAASICNNCVSNRTSMGSVMDFLRTNPGA-NV 494

Query: 526 LNCSTDECATADRHFEQVSLEADQPPQPLVDSTPKVSSQHYQQNSVHENQALGSRSFRRS 585
           + C  + C      FE++++            T     + ++     E++ L S     +
Sbjct: 495 IQCCRNGCGETHGRFEEITIR----------ETEVRLRELWKLQQQQESRELSSIDSGHN 554

Query: 586 HSDQDQHYKKTGQSIAVGDNKEKLLDSKATVIARTSLRSLLGSRSLSTV-SSYCE---DK 615
           HS++++      +   + ++ E +   +    A   +  + GS S S + S YC+   +K
Sbjct: 555 HSEEEE------EEEVLYEDPENMFSCE----AGNEINHISGSGSFSFMPSKYCDEPSEK 602

BLAST of Lag0035717 vs. TAIR 10
Match: AT5G28080.2 (Protein kinase superfamily protein )

HSP 1 Score: 567.0 bits (1460), Expect = 1.8e-161
Identity = 289/437 (66.13%), Postives = 339/437 (77.57%), Query Frame = 0

Query: 18  DFSHYVEIDPTRRYGRYDQILGKGASKTVYRAFDEFEGIEVAWNQVKLCNFLQCPEDLER 77
           D+S YVE+DPT RYGRY+++LGKG+SKTVYR FDE++GIEVAWNQVKL +FLQ P++LER
Sbjct: 11  DYSEYVEVDPTGRYGRYNEVLGKGSSKTVYRGFDEYQGIEVAWNQVKLYDFLQSPQELER 70

Query: 78  LYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTLRQYRLKHRRVNVRAVKR 137
           LY EIHLLKTLKHK+IMKFY SWVDT NRNINFVTEMFTSGTLRQYRLKH+RVN+RAVK 
Sbjct: 71  LYCEIHLLKTLKHKSIMKFYASWVDTDNRNINFVTEMFTSGTLRQYRLKHKRVNIRAVKN 130

Query: 138 WCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGT 197
           WCRQILRGL YLH+ +PPVIHRDLKCDNIF+NGNQGE+KIGDLGLAA L+ SHA HCVGT
Sbjct: 131 WCRQILRGLNYLHTHDPPVIHRDLKCDNIFINGNQGEVKIGDLGLAACLQHSHAAHCVGT 190

Query: 198 PEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYK 257
           PEFMAPEVY+E YN+LVDIYSFGMCVLEMVTF+YPYSEC+HP QIYK+VISGKKPD L K
Sbjct: 191 PEFMAPEVYKEEYNQLVDIYSFGMCVLEMVTFDYPYSECSHPAQIYKRVISGKKPDGLDK 250

Query: 258 VKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCESKSKLSDFR--EELDGIASTTA 317
           VKDP +R F+EKCLA VS RLSA ELL D FL ID+       SD R  E   G+     
Sbjct: 251 VKDPEVRGFIEKCLATVSLRLSACELLDDHFLCIDE-------SDMRRVESEKGLIDEAG 310

Query: 318 QPFLEQE--KSLSSINYSLEASDEWRYLTVPTVP----DGIELFEDEDNDQLE----NLD 377
            P          S+  YSL   ++W Y    TV     D +E   D+D ++ +    ++D
Sbjct: 311 TPLRHSYHIPHYSNGYYSLYNQNQWDYNGDETVESHEIDLLEFQNDDDEEEEDKRFGSVD 370

Query: 378 NSIKGKIREDG-SIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITDQDVI 437
            SIKGK R++G  + LRL+   KEG +RNIYFPFD + DTA++VA EM+ EL++ D+DV 
Sbjct: 371 ISIKGKRRDNGDGLFLRLKTVNKEGCVRNIYFPFDIETDTAISVAREMVEELEMDDRDVT 430

Query: 438 KIAEKIDGEIASLVPEW 442
           KIA  IDGEIASLVP W
Sbjct: 431 KIANMIDGEIASLVPNW 440

BLAST of Lag0035717 vs. TAIR 10
Match: AT3G22420.1 (with no lysine (K) kinase 2 )

HSP 1 Score: 565.1 bits (1455), Expect = 7.0e-161
Identity = 283/426 (66.43%), Postives = 333/426 (78.17%), Query Frame = 0

Query: 18  DFSHYVEIDPTRRYGRYDQILGKGASKTVYRAFDEFEGIEVAWNQVKLCNFLQCPEDLER 77
           D S +VEIDP+ RYGRYD+ILGKGASKTVYRAFDE+EGIEVAWNQVKL NF + PE+LE+
Sbjct: 10  DCSVFVEIDPSGRYGRYDEILGKGASKTVYRAFDEYEGIEVAWNQVKLRNFTRNPEELEK 69

Query: 78  LYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTLRQYRLKHRRVNVRAVKR 137
            + EIHLLKTL H+NIMKFYTSWVDT N +INFVTE+FTSGTLRQYRL+HRRVN+RAVK+
Sbjct: 70  FFREIHLLKTLNHQNIMKFYTSWVDTNNLSINFVTELFTSGTLRQYRLRHRRVNIRAVKQ 129

Query: 138 WCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGT 197
           WC+QIL+GLLYLHS++PP+IHRDLKCDNIF+NGNQGE+KIGDLGLAAILRKSHA  CVGT
Sbjct: 130 WCKQILKGLLYLHSRSPPIIHRDLKCDNIFINGNQGEVKIGDLGLAAILRKSHAVRCVGT 189

Query: 198 PEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYK 257
           PEFMAPEVY+E YNELVD+Y+FGMCVLEMVTF+YPYSEC HP QIYKKV SGKKP+A Y 
Sbjct: 190 PEFMAPEVYDEEYNELVDVYAFGMCVLEMVTFDYPYSECTHPAQIYKKVTSGKKPEAFYL 249

Query: 258 VKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQIDDCES-KSKLSDFREELDGIASTTAQ 317
           VKDP +R+FVEKCLA V+CRL+A ELL DPFLQ D+ +    +  D+    D        
Sbjct: 250 VKDPEVREFVEKCLANVTCRLTALELLQDPFLQDDNMDGFVMRPIDYYNGYDETGVFLRH 309

Query: 318 PFLEQEKSLSSINYSLEASDEWRYLTVPTVPDGIELFEDEDNDQLENLDNSIKGKIREDG 377
           P ++          S                  I+LF ++D D   ++D SIKGK   D 
Sbjct: 310 PLIDDPLYHDQFESSQICE--------------IDLFANDDED---HVDISIKGKRNGDD 369

Query: 378 SIVLRLRIAEKEGHIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEIAS 437
            I LRLRI++ EG IRNIYFPF+T  DTA +VA EM++ELDIT+QDV KIAE ID EIA+
Sbjct: 370 GIFLRLRISDAEGRIRNIYFPFETAIDTAWSVAVEMVSELDITNQDVAKIAEMIDAEIAA 418

Query: 438 LVPEWK 443
           LVP+WK
Sbjct: 430 LVPDWK 418

BLAST of Lag0035717 vs. TAIR 10
Match: AT3G22420.2 (with no lysine (K) kinase 2 )

HSP 1 Score: 533.9 bits (1374), Expect = 1.7e-151
Identity = 283/485 (58.35%), Postives = 333/485 (68.66%), Query Frame = 0

Query: 18  DFSHYVEIDPTRRYGRYDQILGKGASKTVYRAFDEFEGIEVAWNQVKLCNFLQCPEDLER 77
           D S +VEIDP+ RYGRYD+ILGKGASKTVYRAFDE+EGIEVAWNQVKL NF + PE+LE+
Sbjct: 10  DCSVFVEIDPSGRYGRYDEILGKGASKTVYRAFDEYEGIEVAWNQVKLRNFTRNPEELEK 69

Query: 78  LYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTLRQYRLKHRRVNVRAVKR 137
            + EIHLLKTL H+NIMKFYTSWVDT N +INFVTE+FTSGTLRQYRL+HRRVN+RAVK+
Sbjct: 70  FFREIHLLKTLNHQNIMKFYTSWVDTNNLSINFVTELFTSGTLRQYRLRHRRVNIRAVKQ 129

Query: 138 WCRQILRGLLYLHSQNPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCV-- 197
           WC+QIL+GLLYLHS++PP+IHRDLKCDNIF+NGNQGE+KIGDLGLAAILRKSHA  CV  
Sbjct: 130 WCKQILKGLLYLHSRSPPIIHRDLKCDNIFINGNQGEVKIGDLGLAAILRKSHAVRCVGT 189

Query: 198 ------------------------------GTPEFMAPEVYEEAYNELVDIYSFGMCVLE 257
                                         GTPEFMAPEVY+E YNELVD+Y+FGMCVLE
Sbjct: 190 SKPSHHWNFIALIMFFTTLDLPLLCLCVVKGTPEFMAPEVYDEEYNELVDVYAFGMCVLE 249

Query: 258 MVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLS 317
           MVTF+YPYSEC HP QIYKKV SGKKP+A Y VKDP +R+FVEKCLA V+CRL+A ELL 
Sbjct: 250 MVTFDYPYSECTHPAQIYKKVTSGKKPEAFYLVKDPEVREFVEKCLANVTCRLTALELLQ 309

Query: 318 DPFLQIDDCES-KSKLSDFREELDGIASTTAQPFLEQEKSLSSINYSLEASDEWRYLTVP 377
           DPFLQ D+ +    +  D+    D        P ++          S             
Sbjct: 310 DPFLQDDNMDGFVMRPIDYYNGYDETGVFLRHPLIDDPLYHDQFESSQICE--------- 369

Query: 378 TVPDGIELFEDEDNDQLENLDNSIKGKIREDGSIVLRLRIAEKE---------------- 437
                I+LF ++D D   ++D SIKGK   D  I LRLRI++ E                
Sbjct: 370 -----IDLFANDDED---HVDISIKGKRNGDDGIFLRLRISDAEGIVSIFFDSFKIIGLK 429

Query: 438 -----------GHIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEIASL 443
                      G IRNIYFPF+T  DTA +VA EM++ELDIT+QDV KIAE ID EIA+L
Sbjct: 430 NVTRFRVLGNVGRIRNIYFPFETAIDTAWSVAVEMVSELDITNQDVAKIAEMIDAEIAAL 477

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6588353.12.8e-29784.93putative serine/threonine-protein kinase WNK1, partial [Cucurbita argyrosperma s... [more]
XP_023001906.13.7e-29784.93probable serine/threonine-protein kinase WNK9 [Cucurbita maxima][more]
XP_038880702.14.8e-29784.49probable serine/threonine-protein kinase WNK9 isoform X1 [Benincasa hispida][more]
XP_022933886.18.3e-29784.76probable serine/threonine-protein kinase WNK9 isoform X1 [Cucurbita moschata][more]
XP_023529957.11.1e-29684.93probable serine/threonine-protein kinase WNK9 isoform X1 [Cucurbita pepo subsp. ... [more]
Match NameE-valueIdentityDescription
A2YMV67.0e-18260.77Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. indica OX=3... [more]
Q0D5987.0e-18260.77Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. japonica OX... [more]
Q9CAV62.3e-18054.45Serine/threonine-protein kinase WNK1 OS=Arabidopsis thaliana OX=3702 GN=WNK1 PE=... [more]
Q2V3382.6e-16066.13Probable serine/threonine-protein kinase WNK9 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q8S8Y99.9e-16066.43Serine/threonine-protein kinase WNK2 OS=Arabidopsis thaliana OX=3702 GN=WNK2 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1KJY61.8e-29784.93Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1F0994.0e-29784.76Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A1S3CI455.2e-29785.14Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10350... [more]
A0A0A0M0J21.2e-29384.33Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_1... [more]
A0A6J1DKV61.2e-29083.14Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L... [more]
Match NameE-valueIdentityDescription
AT3G04910.11.6e-18154.45with no lysine (K) kinase 1 [more]
AT3G04910.22.5e-16653.43with no lysine (K) kinase 1 [more]
AT5G28080.21.8e-16166.13Protein kinase superfamily protein [more]
AT3G22420.17.0e-16166.43with no lysine (K) kinase 2 [more]
AT3G22420.21.7e-15158.35with no lysine (K) kinase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 32..289
e-value: 2.2E-59
score: 213.3
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 35..289
e-value: 6.7E-49
score: 166.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 32..289
score: 38.505974
NoneNo IPR availableGENE3D3.10.20.90coord: 376..475
e-value: 1.6E-5
score: 27.2
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 18..113
e-value: 7.8E-32
score: 111.2
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 115..295
e-value: 1.7E-49
score: 169.8
NoneNo IPR availablePIRSRPIRSR000606-51PIRSR000606-51coord: 80..302
e-value: 2.6E-19
score: 66.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 516..543
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 507..561
NoneNo IPR availablePANTHERPTHR13902:SF144SERINE/THREONINE-PROTEIN KINASE WNK1coord: 14..616
NoneNo IPR availablePANTHERPTHR13902SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATEDcoord: 14..616
NoneNo IPR availableCDDcd13983STKc_WNKcoord: 30..289
e-value: 2.31863E-171
score: 485.577
IPR024678Serine/threonine-protein kinase OSR1/WNK, CCT domainPFAMPF12202OSR1_Ccoord: 379..433
e-value: 3.3E-5
score: 23.8
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 156..168
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 25..290

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0035717.1Lag0035717.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity