Lag0035606 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0035606
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	membrane protein of ER body-like protein
Location	chr3: 25244947 .. 25246499 (-)
RNA-Seq Expression	Lag0035606
Synteny	Lag0035606

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGGGAGATTCCCATCAATCACAGACCTTCTGTGTATCTTGGTATACAGGTAGGGGGAGTTACTTTAACTTCCCCACTAAGCCCTTTTTTTCTCTCCCCACTACCTGGACACACATGTGGGTAGGTTTATTTTTATTTATTTTTTATTTTTATATTTAATTTAAAGTAATTAATAATTAATATTAATAATAATAATAAATATTAATTAATTTAATTAATAATTAATTAAACCATTTAATTAATTATTATACATTAAATTAATCAAACTAATAATAACTTGTGTATAAAACACTTACGAGGAAATTTAAACAAACATATTAGTTAAATTCTAAAAAACTTTTATACAAAATGTTTAAAATAAAATGCATTCTTGAAAAACACTCAAACTAAAAGCTATTCCAAATGGTCAGCTGATTGGTTGTTATTTTGTATTTTGGTGGTGAAGGTTTAGGCCCGTTTGATAACCATTTGGTTTTTTGTTTTTGGTTTTTGAAAATTATGTTTGTTTTCATCCAATTTTCTTATCTTGGTTTTCATCCTTGTTAAAGACATACTTGAGTTCCTAGCCAAATTTCAAAAACAAAAACAAGTTTTTGAAAACTATTGGTTTTTGAAAACTTTTGAAAACATGAAAATGAGATAGATAACAAAACAAAGAAACTTGTAGATGCATGTAGTGTTTTTAAGCTTAATTTCAAAAACCAAAAACCAGAAACCAAATGGTTATCAAACGTGGTCTTAGTATTGGGTTTACTTGTTTAGATACCCAAACCTCGTATCTTTAGTCATTTTCAATTAAAAAAAAAAAAGTCTCTCTCATTTGGTTTACACCACACAATTCTAATAAATGATTTTGTCTAAGTTACCCTTAAAATAATGTTTACTAGTTTAAAAAACGAAATTCTTTGATATGTAATGAAATTGAACACCATCACAATTTTTTTTTTAAAAAAAAGTATGCTTTGTGTGCTGTTTATGAATTAGATAAAGAAAAATATCATCAATAATAAATCAAAAAACATCCTTTGTTTAATTCTAATAGATAGCTAGATATACACTCTGAAAACAACCATTCCTTGCTATTATAAATCATAATAAATGTTAGATTCTTTCACTTCTATTGTTTATGAGGAATTTCACAGTTATCAGGACTGAAGAATAGGTTGGAGAAGGAGTATAATGGAGCTGAAGTAGAAGGAAGACACCACTACAGAGAAGAAGTGATAGATACAATGCATTATCTTCTCCATTTTATCATTGCTTTCTTATCCTTCCTATTTTGTGGCTTAGTGCCTCCATTGGTTTATGCATTCTCATTTCCTAAGAGTGGCAACAAGAATTTCAAGATCATAGCCACTATGGGAGCTTCCCTTTTGTGCACTACAATGCTTGCCATTCTAAAAGTGCACATTCAGAAACCAAAAGAATGGGGTATCTATGTCAAAACTGCAGCCACTTACATTTCAATTGGAGCTGTAGCCTTCAGTTTATCATACCTAGCAGGTAACATGATTGTAAAGCTCACAGAAAAGTTTCCATGGGAATTTTAG

mRNA sequence

ATGTGGGGAGATTCCCATCAATCACAGACCTTCTGTGTATCTTGGTATACAGGACTGAAGAATAGGTTGGAGAAGGAGTATAATGGAGCTGAAGTAGAAGGAAGACACCACTACAGAGAAGAAGTGATAGATACAATGCATTATCTTCTCCATTTTATCATTGCTTTCTTATCCTTCCTATTTTGTGGCTTAGTGCCTCCATTGGTTTATGCATTCTCATTTCCTAAGAGTGGCAACAAGAATTTCAAGATCATAGCCACTATGGGAGCTTCCCTTTTGTGCACTACAATGCTTGCCATTCTAAAAGTGCACATTCAGAAACCAAAAGAATGGGGTATCTATGTCAAAACTGCAGCCACTTACATTTCAATTGGAGCTGTAGCCTTCAGTTTATCATACCTAGCAGGTAACATGATTGTAAAGCTCACAGAAAAGTTTCCATGGGAATTTTAG

Coding sequence (CDS)

ATGTGGGGAGATTCCCATCAATCACAGACCTTCTGTGTATCTTGGTATACAGGACTGAAGAATAGGTTGGAGAAGGAGTATAATGGAGCTGAAGTAGAAGGAAGACACCACTACAGAGAAGAAGTGATAGATACAATGCATTATCTTCTCCATTTTATCATTGCTTTCTTATCCTTCCTATTTTGTGGCTTAGTGCCTCCATTGGTTTATGCATTCTCATTTCCTAAGAGTGGCAACAAGAATTTCAAGATCATAGCCACTATGGGAGCTTCCCTTTTGTGCACTACAATGCTTGCCATTCTAAAAGTGCACATTCAGAAACCAAAAGAATGGGGTATCTATGTCAAAACTGCAGCCACTTACATTTCAATTGGAGCTGTAGCCTTCAGTTTATCATACCTAGCAGGTAACATGATTGTAAAGCTCACAGAAAAGTTTCCATGGGAATTTTAG

Protein sequence

MWGDSHQSQTFCVSWYTGLKNRLEKEYNGAEVEGRHHYREEVIDTMHYLLHFIIAFLSFLFCGLVPPLVYAFSFPKSGNKNFKIIATMGASLLCTTMLAILKVHIQKPKEWGIYVKTAATYISIGAVAFSLSYLAGNMIVKLTEKFPWEF

Homology

[Close]

BLAST of Lag0035606 vs. NCBI nr
Match: XP_022158259.1 (uncharacterized protein LOC111024792 [Momordica charantia])

HSP 1 Score: 125.6 bits (314), Expect = 3.7e-25
Identity = 61/121 (50.41%), Postives = 85/121 (70.25%), Query Frame = 0


Query: 31  EVEGRHH---YREEVIDTMHYLLHFIIAFLSFLFCGLVPPLVYAFSFPKSGNKNFKIIAT 90
           E + + H   Y E + +  +Y+LH+++A LSF+  G+VPPLVY FSF KS +K+FK++A 
Sbjct: 794 ETDDQQHVDRYEEALGNRENYILHYMVAILSFVIFGMVPPLVYGFSFTKSNDKDFKLLAV 853

Query: 91  MGASLLCTTMLAILKVHIQKPKEWGIYVKTAATYISIGAVAFSLSYLAGNMIVKLTEKFP 149
            GASLLC T+LAI K H Q+P  W +YVKT ATY+ I A A   SYLAG++I +L +K+ 
Sbjct: 854 AGASLLCITLLAIGKAHTQRPNRWDVYVKTVATYVIIAAGAGGFSYLAGDLIDRLIKKYG 913

[Close]

BLAST of Lag0035606 vs. NCBI nr
Match: XP_038897352.1 (uncharacterized protein LOC120085457 isoform X1 [Benincasa hispida] >XP_038897353.1 uncharacterized protein LOC120085457 isoform X1 [Benincasa hispida] >XP_038897354.1 uncharacterized protein LOC120085457 isoform X1 [Benincasa hispida])

HSP 1 Score: 124.0 bits (310), Expect = 1.1e-24
Identity = 66/120 (55.00%), Postives = 85/120 (70.83%), Query Frame = 0


Query: 20  KNRLEKEYNGAEVEGRHHYREEVIDTMHYLLHFIIAFLSFLFCGLVPPLVYAFSFPKSGN 79
           K R  K  +  EVEG + Y+E + + ++YL +F+IAFLSFLF GLVPPLVYA S  K+ N
Sbjct: 765 KRRNNKGCSRNEVEG-NQYKEALKEKLYYLFNFMIAFLSFLFFGLVPPLVYALSSLKTTN 824

Query: 80  KNFKIIATMGASLLCTTMLAILKVHI-QKPKEWGIYVKTAATYISIGAVAFSLSYLAGNM 139
           KN KI+A  GASL CT  LAI K HI QKP+ W +Y+KTA  Y+S+G  AF ++Y+AG M
Sbjct: 825 KNVKIVAAAGASLPCTAFLAIQKAHISQKPRNWNVYIKTAGIYVSVGVGAFGIAYVAGYM 883

[Close]

BLAST of Lag0035606 vs. NCBI nr
Match: XP_008458056.1 (PREDICTED: uncharacterized protein LOC103497596 isoform X1 [Cucumis melo])

HSP 1 Score: 121.3 bits (303), Expect = 7.0e-24
Identity = 64/134 (47.76%), Postives = 88/134 (65.67%), Query Frame = 0


Query: 17  TGLKNRLEKEYNGAEVEGRHHYREEVI--DTMHYLLHFIIAFLSFLFCGLVPPLVYAFSF 76
           TGLK+   +  N  + +  H  R EV+  +  +Y+LHF++A  SF+  GLVPPLVY FSF
Sbjct: 644 TGLKSEQFRTSNETD-DDDHVDRYEVVLGNRENYILHFVLAIFSFVLFGLVPPLVYGFSF 703

Query: 77  PKSGNKNFKIIATMGASLLCTTMLAILKVHIQKPKEWGIYVKTAATYISIGAVAFSLSYL 136
            KS +K+ K+ A  GASLLC T+LA+ K +IQ+P  W +Y+KT  +YI I A A   SYL
Sbjct: 704 TKSNDKDLKLTAVAGASLLCITLLALGKAYIQRPNRWDVYIKTVVSYIVIAAGAGGFSYL 763

Query: 137 AGNMIVKLTEKFPW 149
           AGN+I K  +K+ W
Sbjct: 764 AGNLIDKCVKKYGW 776

[Close]

BLAST of Lag0035606 vs. NCBI nr
Match: XP_004149777.1 (uncharacterized protein LOC101210214 isoform X1 [Cucumis sativus])

HSP 1 Score: 121.3 bits (303), Expect = 7.0e-24
Identity = 63/133 (47.37%), Postives = 88/133 (66.17%), Query Frame = 0


Query: 17  TGLKNRLEKEYNGAEVEGRHHYREEVI-DTMHYLLHFIIAFLSFLFCGLVPPLVYAFSFP 76
           TGLK+   +  N  + + R    E V+ +  +Y+LHF++A  SF+F GLVPPLVY FSF 
Sbjct: 643 TGLKSEQFRTSNETDDDDRVDRYEVVLGNRENYILHFVLAIFSFVFFGLVPPLVYGFSFT 702

Query: 77  KSGNKNFKIIATMGASLLCTTMLAILKVHIQKPKEWGIYVKTAATYISIGAVAFSLSYLA 136
           KS +K+ K+ A  GASLLC  +LA+ K +IQ+P  W +Y+KT A+YI I A A   SYLA
Sbjct: 703 KSNDKDLKLAAVAGASLLCIILLALGKAYIQRPNRWDVYIKTVASYIVIAAGAGGFSYLA 762

Query: 137 GNMIVKLTEKFPW 149
           G++I K  +K+ W
Sbjct: 763 GSLIDKCIKKYGW 775

[Close]

BLAST of Lag0035606 vs. NCBI nr
Match: XP_008458057.1 (PREDICTED: uncharacterized protein LOC103497596 isoform X2 [Cucumis melo] >TYK11596.1 membrane protein of ER body-like protein isoform X4 [Cucumis melo var. makuwa])

HSP 1 Score: 121.3 bits (303), Expect = 7.0e-24
Identity = 64/134 (47.76%), Postives = 88/134 (65.67%), Query Frame = 0


Query: 17  TGLKNRLEKEYNGAEVEGRHHYREEVI--DTMHYLLHFIIAFLSFLFCGLVPPLVYAFSF 76
           TGLK+   +  N  + +  H  R EV+  +  +Y+LHF++A  SF+  GLVPPLVY FSF
Sbjct: 641 TGLKSEQFRTSNETD-DDDHVDRYEVVLGNRENYILHFVLAIFSFVLFGLVPPLVYGFSF 700

Query: 77  PKSGNKNFKIIATMGASLLCTTMLAILKVHIQKPKEWGIYVKTAATYISIGAVAFSLSYL 136
            KS +K+ K+ A  GASLLC T+LA+ K +IQ+P  W +Y+KT  +YI I A A   SYL
Sbjct: 701 TKSNDKDLKLTAVAGASLLCITLLALGKAYIQRPNRWDVYIKTVVSYIVIAAGAGGFSYL 760

Query: 137 AGNMIVKLTEKFPW 149
           AGN+I K  +K+ W
Sbjct: 761 AGNLIDKCVKKYGW 773

[Close]

BLAST of Lag0035606 vs. ExPASy Swiss-Prot
Match: Q8LPT3 (Membrane protein of ER body-like protein OS=Arabidopsis thaliana OX=3702 GN=MEBL PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 8.6e-17
Identity = 47/125 (37.60%), Postives = 73/125 (58.40%), Query Frame = 0


Query: 24  EKEYNGAEVEGRHHYREEVIDTMHYLLHFIIAFLSFLFCGLVPPLVYAFSFPKSGNKNFK 83
           + + NG E E    Y+  +    ++ LH  +A LSF+  G++PP+VY FSF +  NK++K
Sbjct: 611 DNQTNGRE-EEEGRYKRLLGRRENFTLHATVAILSFIITGILPPVVYYFSFSEKHNKDYK 670

Query: 84  IIATMGASLLCTTMLAILKVHIQKPKEWGIYVKTAATYISIGAVAFSLSYLAGNMIVKLT 143
           + +  GASL C  +LAI K H++ P+  G Y+K+   Y SI      +SY+ GN + +L 
Sbjct: 671 VASVFGASLFCIVLLAIAKAHVRYPR--GSYLKSILYYGSIAVSVSGISYVVGNFLEQLL 730

Query: 144 EKFPW 149
           EK  W
Sbjct: 731 EKHGW 732

[Close]

BLAST of Lag0035606 vs. ExPASy Swiss-Prot
Match: F4KFS7 (Membrane protein of ER body 2 OS=Arabidopsis thaliana OX=3702 GN=MEB2 PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 6.2e-15
Identity = 40/100 (40.00%), Postives = 67/100 (67.00%), Query Frame = 0


Query: 50  LHFIIAFLSFLFCGLVPPLVYAFSFPKSGNKNFKIIATMGASLLCTTMLAILKVHIQKPK 109
           +H ++A +S++F GL+PPLVYAFSF ++G KN+K+I+    SL+C  +L  +KV+++KP 
Sbjct: 423 IHILVAVMSYIFFGLIPPLVYAFSFYETGIKNYKLISVFLGSLVCVILLGSIKVYVRKPT 482

Query: 110 ----EWGIYVKTAATYISIGAVAFSLSYLAGNMIVKLTEK 146
                   Y+K+AA Y SI   +  +SY+ G+++ +  EK
Sbjct: 483 NSCGSTKAYLKSAAYYTSIVVASCGISYVVGDIMGEYIEK 522

[Close]

BLAST of Lag0035606 vs. ExPASy Swiss-Prot
Match: Q8W4P8 (Membrane protein of ER body 1 OS=Arabidopsis thaliana OX=3702 GN=MEB1 PE=1 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.1e-11
Identity = 44/118 (37.29%), Postives = 62/118 (52.54%), Query Frame = 0


Query: 31  EVEGRHHYREEVIDTMHY-LLHFIIAFLSFLFCGLVPPLVYAFSFPKSGNK--NFKIIAT 90
           E EG     EEV+    Y  +H +IA  SF+  GL+PPLVY FSF K   K   +K++A 
Sbjct: 484 EGEGEEDRYEEVLGRREYSRIHRVIAISSFVIFGLIPPLVYGFSFRKKMEKRQEYKVLAV 543

Query: 91  MGASLLCTTMLAILKVHIQKPKEWGIYVKTAATYISIGAVAFSLSYLAGNMIVKLTEK 146
              SLLC  +L+I K ++ K ++   YVKT   Y +    A   S   G ++ +  EK
Sbjct: 544 YAVSLLCIVLLSIAKAYVSKKRD---YVKTLFRYTTTATTASGFSQFVGYLVSQWLEK 598

The following BLAST results are available for this feature:

BLAST of Lag0035606 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158259.1	3.7e-25	50.41	uncharacterized protein LOC111024792 [Momordica charantia]	[more]
XP_038897352.1	1.1e-24	55.00	uncharacterized protein LOC120085457 isoform X1 [Benincasa hispida] >XP_03889735...	[more]
XP_008458056.1	7.0e-24	47.76	PREDICTED: uncharacterized protein LOC103497596 isoform X1 [Cucumis melo]	[more]
XP_004149777.1	7.0e-24	47.37	uncharacterized protein LOC101210214 isoform X1 [Cucumis sativus]	[more]
XP_008458057.1	7.0e-24	47.76	PREDICTED: uncharacterized protein LOC103497596 isoform X2 [Cucumis melo] >TYK11...	[more]

BLAST of Lag0035606 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LPT3	8.6e-17	37.60	Membrane protein of ER body-like protein OS=Arabidopsis thaliana OX=3702 GN=MEBL...	[more]
F4KFS7	6.2e-15	40.00	Membrane protein of ER body 2 OS=Arabidopsis thaliana OX=3702 GN=MEB2 PE=1 SV=1	[more]
Q8W4P8	1.1e-11	37.29	Membrane protein of ER body 1 OS=Arabidopsis thaliana OX=3702 GN=MEB1 PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR31851:SF64	GLYCINE-RICH PROTEIN FAMILY	coord: 34..148
IPR008217	Ccc1 family	PANTHER	PTHR31851	FE(2+)/MN(2+) TRANSPORTER PCL1	coord: 34..148

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0035606.1	Lag0035606.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030026	cellular manganese ion homeostasis
biological_process	GO:0098662	inorganic cation transmembrane transport
biological_process	GO:0000041	transition metal ion transport
cellular_component	GO:0016020	membrane
molecular_function	GO:0005384	manganese ion transmembrane transporter activity