Lag0035549 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0035549 vs. NCBI nr
Match: PHT70243.1 (hypothetical protein T459_25347 [Capsicum annuum])

HSP 1 Score: 221.9 bits (564), Expect = 8.2e-54
Identity = 111/207 (53.62%), Postives = 137/207 (66.18%), Query Frame = 0

Query: 21  EKPEKFNGSDFKRWQQKMLFYLTTLSLARFLKEDAPVLDEGETNKEKLLALEAWKHTEYL 80
           EK EKF G DFKRWQQKM FYLTTL L RF  EDAP + EG ++KE  + +EAWKH+++L
Sbjct: 24  EKLEKFAGIDFKRWQQKMFFYLTTLWLQRFTSEDAPEVSEGTSDKEHFMIVEAWKHSDFL 83

Query: 81  CRNYILNGLENTLYNVYSGVDSAKA----------------------------------- 140
           CRNYIL+GL++ LY+ YSG  ++K                                    
Sbjct: 84  CRNYILSGLQDDLYSFYSGTKTSKKLWGALEWKYKTEDAGIKKFFVARYMEFDIAKTPLD 143

Query: 141 -----RSTMESEFIALDKAGEEVEWLRNFLEDIPNWTKPVPPICIHCDSQAAIGRAQNLM 188
                R   E EFIALDKAGEE EW +NFLEDIP WTKPV P+CI+CDSQAAIGRA ++M
Sbjct: 144 VSFTHRKNEEYEFIALDKAGEEAEWQQNFLEDIPYWTKPVAPVCIYCDSQAAIGRAGSMM 203

BLAST of Lag0035549 vs. NCBI nr
Match: KAF3616341.1 (hypothetical protein FXO38_34599 [Capsicum annuum])

HSP 1 Score: 189.1 bits (479), Expect = 5.9e-44
Identity = 108/256 (42.19%), Postives = 133/256 (51.95%), Query Frame = 0

Query: 21  EKPEKFNGSDFKRWQQKMLFYLTTLSLARFLKEDAPVLDEGETNKEKLLALEAWKHTEYL 80
           EKP+KF   +FKRWQQKM FYLTTL L  F  +D P + E  ++KE+ + +E WKH+++L
Sbjct: 35  EKPKKFADINFKRWQQKMFFYLTTLCLQWFTGKDVPEVPEETSDKERFVIVEVWKHSDFL 94

Query: 81  CRNYILNGLENTLYNVYS------------------------------------------ 140
           CRN IL+GL++ LYNVYS                                          
Sbjct: 95  CRNSILSGLQDDLYNVYSLIVNDAFQVAAIIEKLPPLWKEFKNYLKHKRKEMTFEILIVR 154

Query: 141 ------------GVDSAK-----------------------------------ARSTMES 188
                        +DS                                     ARS ME 
Sbjct: 155 LQCNLVGNPRKWWMDSGATCHVCANKELFSLFAPAQVEEMIYMANSATAKTCIARSIMEF 214

BLAST of Lag0035549 vs. NCBI nr
Match: KAF5931719.1 (hypothetical protein HYC85_027890 [Camellia sinensis])

HSP 1 Score: 182.2 bits (461), Expect = 7.2e-42
Identity = 116/330 (35.15%), Postives = 138/330 (41.82%), Query Frame = 0

Query: 8   TPSPIPQGGGNHGEKPEKFNGSDFKRWQQKMLFYLTTLSLARFLKEDAPVLDEGETNKEK 67
           TPSP+     NHGEKPEKF+G+DFKRWQQKMLFYLTTL+LARFL+EDAP+L E +T+++ 
Sbjct: 138 TPSPVVTVPVNHGEKPEKFSGTDFKRWQQKMLFYLTTLNLARFLREDAPILKEDKTDRQV 197

Query: 68  LLALEAWKHTEYLCRNYILNGLENTLYNVYSGVDSAK----------------------- 127
           + A++AWKH ++LCRNY+LN L+N LYNVYS + +AK                       
Sbjct: 198 VAAVDAWKHADFLCRNYVLNRLDNKLYNVYSPIKTAKKLWESLEKKYKTEDAGSKKFVVG 257

Query: 128 ------------------------------------------------------------ 165
                                                                       
Sbjct: 258 RFLDFVIVDSKTVISQAQDFQLILHDIHAEVVFEANLVGNPKEWWVDTGATRHICADKKM 317

BLAST of Lag0035549 vs. NCBI nr
Match: PHT32382.1 (hypothetical protein CQW23_28719 [Capsicum baccatum])

HSP 1 Score: 180.3 bits (456), Expect = 2.7e-41
Identity = 89/167 (53.29%), Postives = 111/167 (66.47%), Query Frame = 0

Query: 21  EKPEKFNGSDFKRWQQKMLFYLTTLSLARFLKEDAPVLDEGETNKEKLLALEAWKHTEYL 80
           EKP+KF G DFKRWQQK+ FYLTT  L RF+ E AP + EG + +EK + +EAWKH++ L
Sbjct: 35  EKPKKFAGIDFKRWQQKIFFYLTTFCLQRFIFEKAPEVPEGTSRQEKFVIVEAWKHSDLL 94

Query: 81  CRNYILNGLENTLYNVYSGVDSAKARSTMESEFIALDKAGEEVEWLRNFLEDIPNWTKPV 140
            RN IL+                             DK GEE +WL+NFLEDIP W K V
Sbjct: 95  YRNNILS-----------------------------DKVGEEAKWLQNFLEDIPYWLKLV 154

Query: 141 PPICIHCDSQAAIGRAQNLMYNGKSRHIRRRHNTIRQLLSNGIISID 188
            P+CIHCDSQAAIGRA ++MYN KS HIRRR+NT+R++LS+GII +D
Sbjct: 155 APVCIHCDSQAAIGRAGSMMYNDKSCHIRRRYNTVREILSSGIIIVD 172

BLAST of Lag0035549 vs. NCBI nr
Match: PHU26485.1 (hypothetical protein BC332_04817 [Capsicum chinense])

HSP 1 Score: 164.5 bits (415), Expect = 1.5e-36
Identity = 101/215 (46.98%), Postives = 119/215 (55.35%), Query Frame = 0

Query: 21  EKPEKFNGSDFKRWQQKMLFYLTTLSLARFLKEDAPV-LDEGET-----NKEKL------ 80
           EKPEKF+G DFKRWQQK+ FYLTTL L RF  EDAP  +D   T     N+E        
Sbjct: 35  EKPEKFSGIDFKRWQQKIFFYLTTLCLQRFTSEDAPEWMDSVATCHVCANRELFSSFAPA 94

Query: 81  ----------LALEAWKHTEYLCRNYILNGLE--NTLYNVYSGVDSAK------------ 140
                      A    + T  +C        E  N+  N+  G + AK            
Sbjct: 95  QVEEMIYMANSATAKVEGTGKICLKMTSGKKEALNSDANLIIGSNEAKSTSGYVFTIGGG 154

Query: 141 ------------ARSTMESEFIALDKAGEEVEWLRNFLEDIPNWTKPVPPICIHCDSQAA 188
                       A STMESEF ALDKA EE EWLRNFLEDI  W  PV P+CIHCDSQA 
Sbjct: 155 SVSWKSSKQTCIAYSTMESEFTALDKANEEAEWLRNFLEDISYWPNPVAPVCIHCDSQAT 214

BLAST of Lag0035549 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 3.2e-08
Identity = 29/89 (32.58%), Postives = 56/89 (62.92%), Query Frame = 0

Query: 98   SGVDSAKARSTMESEFIALDKAGEEVEWLRNFLEDIPNWTKPVPPICIHCDSQAAIGRAQ 157
            S +    A ST E+E+IA  + G+E+ WL+ FL+++    K      ++CDSQ+AI  ++
Sbjct: 1209 SKLQKCVALSTTEAEYIAATETGKEMIWLKRFLQELGLHQK---EYVVYCDSQSAIDLSK 1268

Query: 158  NLMYNGKSRHIRRRHNTIRQLLSNGIISI 187
            N MY+ +++HI  R++ IR+++ +  + +
Sbjct: 1269 NSMYHARTKHIDVRYHWIREMVDDESLKV 1294

BLAST of Lag0035549 vs. ExPASy TrEMBL
Match: A0A2G2YKH8 (Uncharacterized protein OS=Capsicum annuum OX=4072 GN=T459_25347 PE=4 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 4.0e-54
Identity = 111/207 (53.62%), Postives = 137/207 (66.18%), Query Frame = 0

Query: 21  EKPEKFNGSDFKRWQQKMLFYLTTLSLARFLKEDAPVLDEGETNKEKLLALEAWKHTEYL 80
           EK EKF G DFKRWQQKM FYLTTL L RF  EDAP + EG ++KE  + +EAWKH+++L
Sbjct: 24  EKLEKFAGIDFKRWQQKMFFYLTTLWLQRFTSEDAPEVSEGTSDKEHFMIVEAWKHSDFL 83

Query: 81  CRNYILNGLENTLYNVYSGVDSAKA----------------------------------- 140
           CRNYIL+GL++ LY+ YSG  ++K                                    
Sbjct: 84  CRNYILSGLQDDLYSFYSGTKTSKKLWGALEWKYKTEDAGIKKFFVARYMEFDIAKTPLD 143

Query: 141 -----RSTMESEFIALDKAGEEVEWLRNFLEDIPNWTKPVPPICIHCDSQAAIGRAQNLM 188
                R   E EFIALDKAGEE EW +NFLEDIP WTKPV P+CI+CDSQAAIGRA ++M
Sbjct: 144 VSFTHRKNEEYEFIALDKAGEEAEWQQNFLEDIPYWTKPVAPVCIYCDSQAAIGRAGSMM 203

BLAST of Lag0035549 vs. ExPASy TrEMBL
Match: A0A7J7FTW0 ((S)-2-hydroxy-acid oxidase OS=Camellia sinensis OX=4442 GN=HYC85_027890 PE=3 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 3.5e-42
Identity = 116/330 (35.15%), Postives = 138/330 (41.82%), Query Frame = 0

Query: 8   TPSPIPQGGGNHGEKPEKFNGSDFKRWQQKMLFYLTTLSLARFLKEDAPVLDEGETNKEK 67
           TPSP+     NHGEKPEKF+G+DFKRWQQKMLFYLTTL+LARFL+EDAP+L E +T+++ 
Sbjct: 138 TPSPVVTVPVNHGEKPEKFSGTDFKRWQQKMLFYLTTLNLARFLREDAPILKEDKTDRQV 197

Query: 68  LLALEAWKHTEYLCRNYILNGLENTLYNVYSGVDSAK----------------------- 127
           + A++AWKH ++LCRNY+LN L+N LYNVYS + +AK                       
Sbjct: 198 VAAVDAWKHADFLCRNYVLNRLDNKLYNVYSPIKTAKKLWESLEKKYKTEDAGSKKFVVG 257

Query: 128 ------------------------------------------------------------ 165
                                                                       
Sbjct: 258 RFLDFVIVDSKTVISQAQDFQLILHDIHAEVVFEANLVGNPKEWWVDTGATRHICADKKM 317

BLAST of Lag0035549 vs. ExPASy TrEMBL
Match: A0A2G2VHD3 (Uncharacterized protein OS=Capsicum baccatum OX=33114 GN=CQW23_28719 PE=4 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 1.3e-41
Identity = 89/167 (53.29%), Postives = 111/167 (66.47%), Query Frame = 0

Query: 21  EKPEKFNGSDFKRWQQKMLFYLTTLSLARFLKEDAPVLDEGETNKEKLLALEAWKHTEYL 80
           EKP+KF G DFKRWQQK+ FYLTT  L RF+ E AP + EG + +EK + +EAWKH++ L
Sbjct: 35  EKPKKFAGIDFKRWQQKIFFYLTTFCLQRFIFEKAPEVPEGTSRQEKFVIVEAWKHSDLL 94

Query: 81  CRNYILNGLENTLYNVYSGVDSAKARSTMESEFIALDKAGEEVEWLRNFLEDIPNWTKPV 140
            RN IL+                             DK GEE +WL+NFLEDIP W K V
Sbjct: 95  YRNNILS-----------------------------DKVGEEAKWLQNFLEDIPYWLKLV 154

Query: 141 PPICIHCDSQAAIGRAQNLMYNGKSRHIRRRHNTIRQLLSNGIISID 188
            P+CIHCDSQAAIGRA ++MYN KS HIRRR+NT+R++LS+GII +D
Sbjct: 155 APVCIHCDSQAAIGRAGSMMYNDKSCHIRRRYNTVREILSSGIIIVD 172

BLAST of Lag0035549 vs. ExPASy TrEMBL
Match: A0A2N9ILQ6 (Integrase catalytic domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS52911 PE=4 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 8.9e-38
Identity = 86/131 (65.65%), Postives = 97/131 (74.05%), Query Frame = 0

Query: 89  LENTLYNVYSGVDSAKARSTMESEFIALDKAGEEVEWLRNFLEDIPNWTKPVPPICIHCD 148
           LE    +  S   +  ARSTMESEFIALDKAGEE EWLR+FLED+P WTKPVPPICIHCD
Sbjct: 734 LEGNTVSWKSSKQTCIARSTMESEFIALDKAGEEAEWLRHFLEDMPMWTKPVPPICIHCD 793

Query: 149 SQAAIGRAQNLMYNGKSRHIRRRHNTIRQLLSNGIISIDCL-GWSAVTAPETARLDFRRL 208
           SQ+AIGRAQ+ MYNGKSRHIRRRHNT+RQLLSNGIISID +     +  P T  L   R+
Sbjct: 794 SQSAIGRAQSYMYNGKSRHIRRRHNTVRQLLSNGIISIDYIKSKDNIAGPLTKGLSGERV 853

Query: 209 KFSSQLKFTKP 219
             SS+    KP
Sbjct: 854 NCSSRGMGLKP 864

BLAST of Lag0035549 vs. ExPASy TrEMBL
Match: A0A2N9HX08 (Integrase catalytic domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS44135 PE=4 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 2.0e-37
Identity = 83/115 (72.17%), Postives = 92/115 (80.00%), Query Frame = 0

Query: 105 ARSTMESEFIALDKAGEEVEWLRNFLEDIPNWTKPVPPICIHCDSQAAIGRAQNLMYNGK 164
           ARSTMESEFIALDKAGEE EWLR+FLED+P WTKPVPPICIHCDSQ+AIGRAQ+ MYNGK
Sbjct: 854 ARSTMESEFIALDKAGEEAEWLRHFLEDMPMWTKPVPPICIHCDSQSAIGRAQSYMYNGK 913

Query: 165 SRHIRRRHNTIRQLLSNGIISIDCL-GWSAVTAPETARLDFRRLKFSSQLKFTKP 219
           SRHIRRRHNT+RQLLSNGIISID +     +  P T  L   R+  SS+    KP
Sbjct: 914 SRHIRRRHNTVRQLLSNGIISIDYIKSKDNIADPLTKGLSGERINCSSRGMGLKP 968

BLAST of Lag0035549 vs. TAIR 10
Match: AT5G35970.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 52.4 bits (124), Expect = 8.0e-07
Identity = 22/36 (61.11%), Postives = 33/36 (91.67%), Query Frame = 0

Query: 287 KRELGKSVVRWIGKAMRAVASDFASTEDQGDFSKLR 323
           +R+LG++VV+WI +AM+A+ASDFA+ E QG+FS+LR
Sbjct: 116 RRDLGRNVVKWISQAMKAMASDFATAEVQGEFSELR 151

BLAST of Lag0035549 vs. TAIR 10
Match: AT4G00980.1 (zinc knuckle (CCHC-type) family protein )

HSP 1 Score: 45.8 bits (107), Expect = 7.5e-05
Identity = 25/84 (29.76%), Postives = 39/84 (46.43%), Query Frame = 0

Query: 22  KPEKFNGSDFKRWQQKMLFYLTTLSLARFLKEDAPVLDEG---ETNKEKLLALEA----W 81
           K  +F+G  +  W  +M  +L  L L   L E  P +      ETN  ++   +A    W
Sbjct: 194 KISRFDGKSYLYWASQMELFLKQLKLTYVLSEPCPSIGSSQGPETNPREITRADATGKKW 253

Query: 82  KHTEYLCRNYILNGLENTLYNVYS 99
              +YLC  +++N L + LY  YS
Sbjct: 254 LRDDYLCYTHLMNSLSDHLYRRYS 277