Lag0035547 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0035547
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationchr3: 23865914 .. 23866216 (-)
RNA-Seq ExpressionLag0035547
SyntenyLag0035547
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGAATGCAATGATGGCTGGATTGTCTATGCATGGTTGTGGAAAAGAAGCTTTAGAACTCTACTCAAAGATGGAGAGCCATGGTGTTGAACCCAATGATATCACATTCATTTCAGTATTTCACGCTTGTAGTCATTCTGGATTGTTACCAGAAGGGAAGAAGCATTTCAACAGAATGGTTCATGACTTTGGAATTGTTCCAAAGATCGAGCACTATGAATGCTTGGTGGATCTTCTCGGTCGAGCTGGACATCTCGACGAAGCTTACAACATCATTCAAAACATGCCCATGAGGCCGTAA

mRNA sequence

ATGTGGAATGCAATGATGGCTGGATTGTCTATGCATGGTTGTGGAAAAGAAGCTTTAGAACTCTACTCAAAGATGGAGAGCCATGGTGTTGAACCCAATGATATCACATTCATTTCAGTATTTCACGCTTGTAGTCATTCTGGATTGTTACCAGAAGGGAAGAAGCATTTCAACAGAATGGTTCATGACTTTGGAATTGTTCCAAAGATCGAGCACTATGAATGCTTGGTGGATCTTCTCGGTCGAGCTGGACATCTCGACGAAGCTTACAACATCATTCAAAACATGCCCATGAGGCCGTAA

Coding sequence (CDS)

ATGTGGAATGCAATGATGGCTGGATTGTCTATGCATGGTTGTGGAAAAGAAGCTTTAGAACTCTACTCAAAGATGGAGAGCCATGGTGTTGAACCCAATGATATCACATTCATTTCAGTATTTCACGCTTGTAGTCATTCTGGATTGTTACCAGAAGGGAAGAAGCATTTCAACAGAATGGTTCATGACTTTGGAATTGTTCCAAAGATCGAGCACTATGAATGCTTGGTGGATCTTCTCGGTCGAGCTGGACATCTCGACGAAGCTTACAACATCATTCAAAACATGCCCATGAGGCCGTAA

Protein sequence

MWNAMMAGLSMHGCGKEALELYSKMESHGVEPNDITFISVFHACSHSGLLPEGKKHFNRMVHDFGIVPKIEHYECLVDLLGRAGHLDEAYNIIQNMPMRP
Homology
BLAST of Lag0035547 vs. NCBI nr
Match: XP_008462708.1 (PREDICTED: pentatricopeptide repeat-containing protein At3g26782, mitochondrial-like [Cucumis melo])

HSP 1 Score: 198.4 bits (503), Expect = 3.0e-47
Identity = 88/100 (88.00%), Postives = 97/100 (97.00%), Query Frame = 0

Query: 1   MWNAMMAGLSMHGCGKEALELYSKMESHGVEPNDITFISVFHACSHSGLLPEGKKHFNRM 60
           MWNAMMAG SMHGCGKEALEL+S+MESHGVEPNDITFIS+FHACSHSGL+ EGKKHFNRM
Sbjct: 462 MWNAMMAGFSMHGCGKEALELFSEMESHGVEPNDITFISIFHACSHSGLVVEGKKHFNRM 521

Query: 61  VHDFGIVPKIEHYECLVDLLGRAGHLDEAYNIIQNMPMRP 101
           VH+FGIVPK+EHY CLVDLLGRAGHL+EA+NII+NMPMRP
Sbjct: 522 VHNFGIVPKMEHYGCLVDLLGRAGHLEEAHNIIENMPMRP 561

BLAST of Lag0035547 vs. NCBI nr
Match: KAA0062552.1 (pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa] >TYK28774.1 pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 198.0 bits (502), Expect = 3.9e-47
Identity = 88/100 (88.00%), Postives = 96/100 (96.00%), Query Frame = 0

Query: 1   MWNAMMAGLSMHGCGKEALELYSKMESHGVEPNDITFISVFHACSHSGLLPEGKKHFNRM 60
           MWNAMMAG SMHGCGKEALEL+S+MESHGVEPNDITFIS+FHACSHSGL+ EGKKHFNRM
Sbjct: 462 MWNAMMAGFSMHGCGKEALELFSEMESHGVEPNDITFISIFHACSHSGLVVEGKKHFNRM 521

Query: 61  VHDFGIVPKIEHYECLVDLLGRAGHLDEAYNIIQNMPMRP 101
           VH FGIVPK+EHY CLVDLLGRAGHL+EA+NII+NMPMRP
Sbjct: 522 VHSFGIVPKMEHYGCLVDLLGRAGHLEEAHNIIENMPMRP 561

BLAST of Lag0035547 vs. NCBI nr
Match: XP_038879151.1 (pentatricopeptide repeat-containing protein At3g26782, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 197.2 bits (500), Expect = 6.7e-47
Identity = 88/100 (88.00%), Postives = 96/100 (96.00%), Query Frame = 0

Query: 1   MWNAMMAGLSMHGCGKEALELYSKMESHGVEPNDITFISVFHACSHSGLLPEGKKHFNRM 60
           MWNAMMAG SMHGCGKEALEL+S+ME +GVEPNDITFISVFHACSHSGL+ +GKKHFNRM
Sbjct: 463 MWNAMMAGFSMHGCGKEALELFSEMEGYGVEPNDITFISVFHACSHSGLVVDGKKHFNRM 522

Query: 61  VHDFGIVPKIEHYECLVDLLGRAGHLDEAYNIIQNMPMRP 101
           VHDFGIVPKIEHY CLVDLLGRAGHLDEA+NII+NMPM+P
Sbjct: 523 VHDFGIVPKIEHYGCLVDLLGRAGHLDEAHNIIENMPMKP 562

BLAST of Lag0035547 vs. NCBI nr
Match: KAE8653622.1 (hypothetical protein Csa_007111 [Cucumis sativus])

HSP 1 Score: 196.4 bits (498), Expect = 1.1e-46
Identity = 86/100 (86.00%), Postives = 96/100 (96.00%), Query Frame = 0

Query: 1   MWNAMMAGLSMHGCGKEALELYSKMESHGVEPNDITFISVFHACSHSGLLPEGKKHFNRM 60
           MWN MMAG SMHGCGKEALEL+S+MESHGVEPNDITF+S+FHACSHSGL+ EGKK+FN+M
Sbjct: 384 MWNTMMAGFSMHGCGKEALELFSEMESHGVEPNDITFVSIFHACSHSGLVVEGKKYFNKM 443

Query: 61  VHDFGIVPKIEHYECLVDLLGRAGHLDEAYNIIQNMPMRP 101
           VHDFGIVPK+EHY CLVDLLGRAGHLDEA+NII+NMPMRP
Sbjct: 444 VHDFGIVPKMEHYGCLVDLLGRAGHLDEAHNIIENMPMRP 483

BLAST of Lag0035547 vs. NCBI nr
Match: XP_011660280.1 (pentatricopeptide repeat-containing protein At3g26782, mitochondrial [Cucumis sativus])

HSP 1 Score: 196.4 bits (498), Expect = 1.1e-46
Identity = 86/100 (86.00%), Postives = 96/100 (96.00%), Query Frame = 0

Query: 1   MWNAMMAGLSMHGCGKEALELYSKMESHGVEPNDITFISVFHACSHSGLLPEGKKHFNRM 60
           MWN MMAG SMHGCGKEALEL+S+MESHGVEPNDITF+S+FHACSHSGL+ EGKK+FN+M
Sbjct: 461 MWNTMMAGFSMHGCGKEALELFSEMESHGVEPNDITFVSIFHACSHSGLVVEGKKYFNKM 520

Query: 61  VHDFGIVPKIEHYECLVDLLGRAGHLDEAYNIIQNMPMRP 101
           VHDFGIVPK+EHY CLVDLLGRAGHLDEA+NII+NMPMRP
Sbjct: 521 VHDFGIVPKMEHYGCLVDLLGRAGHLDEAHNIIENMPMRP 560

BLAST of Lag0035547 vs. ExPASy Swiss-Prot
Match: Q9LUS3 (Pentatricopeptide repeat-containing protein At3g16610 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E91 PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 3.2e-28
Identity = 51/100 (51.00%), Postives = 72/100 (72.00%), Query Frame = 0

Query: 2   WNAMMAGLSMHGCGKEALELYSKMESHGVEPNDITFISVFHACSHSGLLPEGKKHFNRMV 61
           WN M+ G  +HG GKEAL L++ M+  GV P+++T +++  ACSHSGL+ EGK+ FN M 
Sbjct: 478 WNTMLFGFGIHGLGKEALSLFNSMQETGVNPDEVTLLAILSACSHSGLVDEGKQLFNSMS 537

Query: 62  H-DFGIVPKIEHYECLVDLLGRAGHLDEAYNIIQNMPMRP 101
             DF ++P+I+HY C+ DLL RAG+LDEAY+ +  MP  P
Sbjct: 538 RGDFNVIPRIDHYNCMTDLLARAGYLDEAYDFVNKMPFEP 577

BLAST of Lag0035547 vs. ExPASy Swiss-Prot
Match: Q9LS72 (Pentatricopeptide repeat-containing protein At3g29230 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E27 PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 3.6e-27
Identity = 51/99 (51.52%), Postives = 71/99 (71.72%), Query Frame = 0

Query: 2   WNAMMAGLSMHGCGKEALELYSKMESHGVEPNDITFISVFHACSHSGLLPEGKKHFNRMV 61
           WN M+ GL +HG GKEA+EL+S+M   G+ P+ +TFI+V  +C+H+GL+ EG  +F  M 
Sbjct: 384 WNTMLHGLGVHGHGKEAIELFSRMRREGIRPDKVTFIAVLCSCNHAGLIDEGIDYFYSME 443

Query: 62  HDFGIVPKIEHYECLVDLLGRAGHLDEAYNIIQNMPMRP 101
             + +VP++EHY CLVDLLGR G L EA  ++Q MPM P
Sbjct: 444 KVYDLVPQVEHYGCLVDLLGRVGRLKEAIKVVQTMPMEP 482

BLAST of Lag0035547 vs. ExPASy Swiss-Prot
Match: O82380 (Pentatricopeptide repeat-containing protein At2g29760, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-H33 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 6.1e-27
Identity = 49/100 (49.00%), Postives = 78/100 (78.00%), Query Frame = 0

Query: 1   MWNAMMAGLSMHGCGKEALELYSKMESHGVEPNDITFISVFHACSHSGLLPEGKKHFNRM 60
           +W+AM+ GL+MHGCG EA++++ KM+   V+PN +TF +VF ACSH+GL+ E +  F++M
Sbjct: 433 VWSAMIGGLAMHGCGNEAVDMFYKMQEANVKPNGVTFTNVFCACSHTGLVDEAESLFHQM 492

Query: 61  VHDFGIVPKIEHYECLVDLLGRAGHLDEAYNIIQNMPMRP 101
             ++GIVP+ +HY C+VD+LGR+G+L++A   I+ MP+ P
Sbjct: 493 ESNYGIVPEEKHYACIVDVLGRSGYLEKAVKFIEAMPIPP 532

BLAST of Lag0035547 vs. ExPASy Swiss-Prot
Match: Q9SMZ2 (Pentatricopeptide repeat-containing protein At4g33170 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H53 PE=3 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 8.0e-27
Identity = 53/97 (54.64%), Postives = 71/97 (73.20%), Query Frame = 0

Query: 2   WNAMMAGLSMHGCGKEALELYSKMESHGVEPNDITFISVFHACSHSGLLPEGKKHFNRMV 61
           WNAM+ GL+ HG GKE L+L+ +M+S G++P+ +TFI V  ACSHSGL+ E  KH   M 
Sbjct: 687 WNAMLVGLAQHGEGKETLQLFKQMKSLGIKPDKVTFIGVLSACSHSGLVSEAYKHMRSMH 746

Query: 62  HDFGIVPKIEHYECLVDLLGRAGHLDEAYNIIQNMPM 99
            D+GI P+IEHY CL D LGRAG + +A N+I++M M
Sbjct: 747 GDYGIKPEIEHYSCLADALGRAGLVKQAENLIESMSM 783

BLAST of Lag0035547 vs. ExPASy Swiss-Prot
Match: Q9CA54 (Pentatricopeptide repeat-containing protein At1g74630 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H71 PE=2 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 1.0e-26
Identity = 51/99 (51.52%), Postives = 76/99 (76.77%), Query Frame = 0

Query: 2   WNAMMAGLSMHGCGKEALELYSKMESHGVEPNDITFISVFHACSHSGLLPEGKKHFNRMV 61
           W +M+AGL+MHG G+EA+ L+++M ++GV P+ I+FIS+ HACSH+GL+ EG+ +F+ M 
Sbjct: 339 WTSMIAGLAMHGQGEEAVRLFNEMTAYGVTPDGISFISLLHACSHAGLIEEGEDYFSEMK 398

Query: 62  HDFGIVPKIEHYECLVDLLGRAGHLDEAYNIIQNMPMRP 101
             + I P+IEHY C+VDL GR+G L +AY+ I  MP+ P
Sbjct: 399 RVYHIEPEIEHYGCMVDLYGRSGKLQKAYDFICQMPIPP 437

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008462708.13.0e-4788.00PREDICTED: pentatricopeptide repeat-containing protein At3g26782, mitochondrial-... [more]
KAA0062552.13.9e-4788.00pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa] >TYK28774... [more]
XP_038879151.16.7e-4788.00pentatricopeptide repeat-containing protein At3g26782, mitochondrial-like [Benin... [more]
KAE8653622.11.1e-4686.00hypothetical protein Csa_007111 [Cucumis sativus][more]
XP_011660280.11.1e-4686.00pentatricopeptide repeat-containing protein At3g26782, mitochondrial [Cucumis sa... [more]
Match NameE-valueIdentityDescription
Q9LUS33.2e-2851.00Pentatricopeptide repeat-containing protein At3g16610 OS=Arabidopsis thaliana OX... [more]
Q9LS723.6e-2751.52Pentatricopeptide repeat-containing protein At3g29230 OS=Arabidopsis thaliana OX... [more]
O823806.1e-2749.00Pentatricopeptide repeat-containing protein At2g29760, chloroplastic OS=Arabidop... [more]
Q9SMZ28.0e-2754.64Pentatricopeptide repeat-containing protein At4g33170 OS=Arabidopsis thaliana OX... [more]
Q9CA541.0e-2651.52Pentatricopeptide repeat-containing protein At1g74630 OS=Arabidopsis thaliana OX... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 2..45
e-value: 9.6E-12
score: 44.9
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 1..33
e-value: 9.9E-9
score: 32.9
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 73..97
e-value: 0.07
score: 13.4
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 1..32
score: 11.136739
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 1..100
e-value: 9.8E-25
score: 89.6
NoneNo IPR availablePANTHERPTHR47925:SF61PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN DOT4, CHLOROPLASTICcoord: 2..99
NoneNo IPR availablePANTHERPTHR47925OS01G0913400 PROTEIN-RELATEDcoord: 2..99

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0035547.1Lag0035547.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding
molecular_function GO:0008270 zinc ion binding