Lag0035538 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0035538
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Transposon TX1 uncharacterized 149 kDa protein
Location	chr3: 23704294 .. 23706339 (-)
RNA-Seq Expression	Lag0035538
Synteny	Lag0035538

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGCTTCTTAGATGGAATAAATCAATAAAGGTATCTTCGGAAAATCTTCAATCTCTTATTTCTCAATTAAAGGCTTTGGACAATCTTGAAGATTGTGATTCTTTATCTTCTGACCAAATGTCTCAAAGGACTCATCTTAGAGAACAAATTGAGGATATTACTGCTCGAGAACATACATACTGGAGACAAAGATGTAAGCTTAATTGGTTGAAAGAAGGTGATGAGAATACAAAATTTTTTCATCGTTTTATGGCCGCTCGTAAAAGGAAAAAATCAATTTATGAGTTGTTGTCTCGTAATGGGGTGAGTCTTTTGCAGCTAAAGATATTGAGGAGGAGTTCATTGATTTCTATAAGAAGCTATATACGAAAGATTCAACTTCGCGATTTTTACCTACCAATCTTGAATGGGGTAGGATTAATGCCTCCCAATCATCTGCTCTAGAAACTCCTTTTACTGAAGAGGAGGTTTACTCTGCTGTGCAATCTCTGGGAACTAGTAAATCTCCTGGTCCTGACGGTTTTACTGCTGAATTCTTTAAGAACTCATGGACACTATTAAGCATGACGTTATGGCAATTTTGTATGAGTTTTATGGCAGCGGAATCATTAATGTGGCTCTGAATGAAACTTATATCTGTCTCATTACTAAACGAGTTGATTCTAGAACAATAAATGGTTATCGTCCCATCAGTCTTATTTCTTGTGCTTACAAGATCACTGCTCGTGTTTTATCTAATCAAATTAAGCAAGTCCTTCCTTTCACCATTGTTGAAAATCAAATGGCCTTTGTATTTAATCGACAAATCTTGGATGCTTCATTGATTGCAATGAATTGATTGATGACGGGAAATTCTCTAATGAGAAAGGTGTGGTTATTAAGCTGGATTTGGAGAAGGCATTTGATAAAGTTGATTGGGACTTTTTGGATGCAGTTCTTCAAGCTGAGGGCTTTGGTTGTGTTTGGAGAAAGTGGATTCGGGGTTGTATCTCTAGTGCTAATTATTCGATCATTATTAATGGACGACCTAGAGGTAAAATCATTCCATCTAGGGGAATTCGACAAGGGGATCCACTATCTCCTTTCCTCTTTATACTAGTATCTGATTGTTTAAGTCGATTACTGACCTATAGTTCTCAAATGGGGAAGATTATGACACATCCTGTGGGAATTCCTCTCTAAATGTGAGCCACTTGCAATTTGCAGATGATACTTTGTTATTCTCTACTATTGATTCTTCTGCCATTGACAATCTTTTTATATTATTAAAATTTTGAATTTACATCCGGTTTGAATATCAATTTTGGCAAAAGTGAACGGTTAGGGATTAACGTTGGTGAGTTGGAATTAGAAGTCTTAACTTCAAAGTTTGGGTGTAAAAAAGAAGTCTGGCCAACAACCTATCTTAGTCTTCCCTTAGGCGAAAATCCTAAGTTATCATCATTCTGGCAGCCTGTGATTGAAAGAATCCAGAGAAAGCTTCATAGTTGGAAGTATATGTACATTTCTAAAGGTGGCAGGTACACTCTAATTCATGCTACTCTCTCGAGTATGCCCATTTATTATTTATCTCTGTTTAAATTGCCAGCTAAGGTGGGGGCTTACATAATATCAATTGGGCAAAGACAGAACTCCCTCACATAATGGGAGGCCTTGGTATTGGAAATTTTCAATTAGAAATACTGCACCTTTATCAAAATGGATTTGGAGATTTTTGCATGATCAAGACAGCCTATGGAGAAAAATGATTTTGCTAAGTTTTACAATGCTGCTGGAATTTGTTGGTGGCCAAAGCCCATCATGGGAGGGTCATCAAAATCACTTTGGAAATCTATTTATAATACAATTGATGTTGTGGGTAGTCGAATTAAAAAACGACTTGGCAATGGTATGCATACATCTTTTTGGAATGATATATGGCTTGACAGTGTTCCTCTTAAAATATCATATCCTCGTCTTCATCGGATATCTACCCGACCTATGGATTCAGTTGCTGACGTTTGGAACTCAGCATCTAAAGCATGGGATTTGGGCCTCCGTTGA

mRNA sequence

ATGGAGCTTCTTAGATGGAATAAATCAATAAAGGTATCTTCGGAAAATCTTCAATCTCTTATTTCTCAATTAAAGGCTTTGGACAATCTTGAAGATTGTGATTCTTTATCTTCTGACCAAATGTCTCAAAGGACTCATCTTAGAGAACAAATTGAGGATATTACTGCTCGAGAACATACATACTGGAGACAAAGATCTAAAGATATTGAGGAGGAGTTCATTGATTTCTATAAGAAGCTATATACGAAAGATTCAACTTCGCGATTTTTACCTACCAATCTTGAATGGGGTAGGATTAATGCCTCCCAATCATCTGCTCTAGAAACTCCTTTTACTGAAGAGGAGGTTTACTCTGCTGTGCAATCTCTGGGAACTAGTAAATCTCCTGGTCCTGACGGTTTTACTGCTGAATTCTTTAAGAACTCATGGACACTATTAAGCATGACGTTATGGCAATTTTGTGTGGTTATTAAGCTGGATTTGGAGAAGGCATTTGATAAAGTTGATTGGGACTTTTTGGATGCAGTTCTTCAAGCTGAGGGCTTTGGTTGTGTTTGGAGAAAGTGGATTCGGGGTTGTATCTCTAGTGCTAATTATTCGATCATTATTAATGGACGACCTAGAGGCGAAAATCCTAAGTTATCATCATTCTGGCAGCCTGTGATTGAAAGAATCCAGAGAAAGCTTCATAGTTGGAAGTATATGTACATTTCTAAAGGTGGCAGACAGCCTATGGAGAAAAATGATTTTGCTAAGTTTTACAATGCTGCTGGAATTTGTTGGTGGCCAAAGCCCATCATGGGAGGGTCATCAAAATCACTTTGGAAATCTATTTATAATACAATTGATGTTGTGGGTAGTCGAATTAAAAAACGACTTGGCAATGGTATGCATACATCTTTTTGGAATGATATATGGCTTGACAGTGTTCCTCTTAAAATATCATATCCTCGTCTTCATCGGATATCTACCCGACCTATGGATTCAGTTGCTGACGTTTGGAACTCAGCATCTAAAGCATGGGATTTGGGCCTCCGTTGA

Coding sequence (CDS)

ATGGAGCTTCTTAGATGGAATAAATCAATAAAGGTATCTTCGGAAAATCTTCAATCTCTTATTTCTCAATTAAAGGCTTTGGACAATCTTGAAGATTGTGATTCTTTATCTTCTGACCAAATGTCTCAAAGGACTCATCTTAGAGAACAAATTGAGGATATTACTGCTCGAGAACATACATACTGGAGACAAAGATCTAAAGATATTGAGGAGGAGTTCATTGATTTCTATAAGAAGCTATATACGAAAGATTCAACTTCGCGATTTTTACCTACCAATCTTGAATGGGGTAGGATTAATGCCTCCCAATCATCTGCTCTAGAAACTCCTTTTACTGAAGAGGAGGTTTACTCTGCTGTGCAATCTCTGGGAACTAGTAAATCTCCTGGTCCTGACGGTTTTACTGCTGAATTCTTTAAGAACTCATGGACACTATTAAGCATGACGTTATGGCAATTTTGTGTGGTTATTAAGCTGGATTTGGAGAAGGCATTTGATAAAGTTGATTGGGACTTTTTGGATGCAGTTCTTCAAGCTGAGGGCTTTGGTTGTGTTTGGAGAAAGTGGATTCGGGGTTGTATCTCTAGTGCTAATTATTCGATCATTATTAATGGACGACCTAGAGGCGAAAATCCTAAGTTATCATCATTCTGGCAGCCTGTGATTGAAAGAATCCAGAGAAAGCTTCATAGTTGGAAGTATATGTACATTTCTAAAGGTGGCAGACAGCCTATGGAGAAAAATGATTTTGCTAAGTTTTACAATGCTGCTGGAATTTGTTGGTGGCCAAAGCCCATCATGGGAGGGTCATCAAAATCACTTTGGAAATCTATTTATAATACAATTGATGTTGTGGGTAGTCGAATTAAAAAACGACTTGGCAATGGTATGCATACATCTTTTTGGAATGATATATGGCTTGACAGTGTTCCTCTTAAAATATCATATCCTCGTCTTCATCGGATATCTACCCGACCTATGGATTCAGTTGCTGACGTTTGGAACTCAGCATCTAAAGCATGGGATTTGGGCCTCCGTTGA

Protein sequence

MELLRWNKSIKVSSENLQSLISQLKALDNLEDCDSLSSDQMSQRTHLREQIEDITAREHTYWRQRSKDIEEEFIDFYKKLYTKDSTSRFLPTNLEWGRINASQSSALETPFTEEEVYSAVQSLGTSKSPGPDGFTAEFFKNSWTLLSMTLWQFCVVIKLDLEKAFDKVDWDFLDAVLQAEGFGCVWRKWIRGCISSANYSIIINGRPRGENPKLSSFWQPVIERIQRKLHSWKYMYISKGGRQPMEKNDFAKFYNAAGICWWPKPIMGGSSKSLWKSIYNTIDVVGSRIKKRLGNGMHTSFWNDIWLDSVPLKISYPRLHRISTRPMDSVADVWNSASKAWDLGLR

Homology

[Close]

BLAST of Lag0035538 vs. NCBI nr
Match: RVW26335.1 (Uridylate kinase [Vitis vinifera])

HSP 1 Score: 161.8 bits (408), Expect = 1.1e-35
Identity = 104/317 (32.81%), Postives = 154/317 (48.58%), Query Frame = 0


Query: 2   ELLRWNK-SIKVSSENLQSLISQLKALDNLEDCDSLSSDQMSQRTHLREQIEDITAREHT 61
           +L  WNK +     E    ++S L  +D +E   +L+ D +S+RT  R ++ED+  +E  
Sbjct: 608 KLKDWNKVAFGDLRERKNLILSNLGRIDLIEQEGNLNLDLLSERTLRRRKLEDLLLKEEV 667

Query: 62  YWRQRSK------------DIE---EEFIDFYKKLYTKDSTSRFLPTNLEWGRINASQSS 121
           +WRQ+S+            +IE   EE ++F+ KLY+K   + +    L+W  I+   ++
Sbjct: 668 HWRQKSRVKWIKEGDCNSNNIEVIFEEILNFFGKLYSKPEGASWRVEGLDWVPISRESAA 727

Query: 122 ALETPFTEEEVYSAVQSLGTSKSPGPDGFTAEFFKNSWTLLSMTLW-------------Q 181
            L+ PF EEEV  AV  L   K+ GPDGFT   +++ W ++   L              Q
Sbjct: 728 WLDRPFIEEEVRLAVFQLNKEKALGPDGFTIAVYQDCWNVIKENLMRVFLEFHTNGIINQ 787

Query: 182 FC-------------------------------------------VVIKLDLEKAFDKVD 241
            C                                           VV K+D EKA+D VD
Sbjct: 788 RCLRKVLHETIFGSQGAFVEGRHILDAVLIANEVVDEKRRSGEEGVVFKIDFEKAYDYVD 847

Query: 242 WDFLDAVLQAEGFGCVWRKWIRGCISSANYSIIINGR----PRGENPKLSSFWQPVIERI 243
           W FLD VL+ + F   WR WIRGC+SS++++I++NG     P G NPK   FW PV+ERI
Sbjct: 848 WGFLDHVLERKEFSPKWRSWIRGCLSSSSFAILVNGNAKGFPLGGNPKTIDFWDPVVERI 907

[Close]

BLAST of Lag0035538 vs. NCBI nr
Match: RVW76917.1 (hypothetical protein CK203_051555 [Vitis vinifera])

HSP 1 Score: 154.8 bits (390), Expect = 1.3e-33
Identity = 90/281 (32.03%), Postives = 134/281 (47.69%), Query Frame = 0


Query: 25   KALDNLEDCDSLSSDQMSQRTHLREQIEDITAREHTYWRQRSKDIEEEFIDFYKKLYTKD 84
            K +D +E   +L+ D +S+RT  R+++ED+  +E        + I EE ++F+  LY+K 
Sbjct: 846  KRIDRIEQEGNLNLDLVSERTLRRKELEDLLLKEEGETLNNIEVISEEIVNFFGNLYSKP 905

Query: 85   STSRFLPTNLEWGRINASQSSALETPFTEEEVYSAVQSLGTSKSPGPDGFTAEFFKNSWT 144
                +    ++W  I+   +  L+ PF+EEEV  AV  L   K+P PDGFT   ++  W 
Sbjct: 906  EGDSWKIEGIDWAPISEESAIWLDRPFSEEEVRMAVFQLNKEKAPDPDGFTIAVYQECWD 965

Query: 145  LLSMTL-------------------------------------------WQFCVVIKLDL 204
            ++   L                                            +  VV K+D 
Sbjct: 966  VIKEDLNEGRLRKVLHETIFGSQGAFVEGRQILDAVLIANEVVDEKRRSGEEGVVFKIDF 1025

Query: 205  EKAFDKVDWDFLDAVLQAEGFGCVWRKWIRGCISSANYSIIINGR--------------- 243
            EKA+D V+W FLD VLQ +GF   WR W+RGC+SS++++I++NG                
Sbjct: 1026 EKAYDHVEWGFLDHVLQRKGFSQKWRSWMRGCLSSSSFAILVNGNAKGWVKPLESVKWPL 1085

[Close]

BLAST of Lag0035538 vs. NCBI nr
Match: RVW51835.1 (putative ribonuclease H protein [Vitis vinifera])

HSP 1 Score: 150.2 bits (378), Expect = 3.3e-32
Identity = 108/437 (24.71%), Postives = 171/437 (39.13%), Query Frame = 0


Query: 12  VSSENLQSLISQLKALDNLEDCDSLSSDQMSQRTHLREQIEDITAREHTYWRQ------- 71
           V  E  ++++ ++  +D  E   +LSSD + QR    +       R    + +       
Sbjct: 66  VGKERKKTILDEIANIDANEQERALSSDLVVQRALFHKVANGRRNRNFIKFLENESGLVL 125

Query: 72  -RSKDIEEEFIDFYKKLYTKDSTSRFLPTNLEWGRINASQSSALETPFTEEEVYSAVQSL 131
             S+ I EE + ++KKLY+      +    ++W  I+   +S L++ F+E E+Y+ +  L
Sbjct: 126 DNSESITEEILLYFKKLYSSPPGESWRVEGIDWSPISEESASRLDSLFSEVEIYNVIFQL 185

Query: 132 GTSKSPGPDGFTAEFFKNSWTLLSMTLWQFC----------------------------- 191
              K+ GPDGFT   F++ W ++   L +                               
Sbjct: 186 NRDKASGPDGFTIAMFQDCWDVIKDDLVRVFAEFHNSGIINQNTNASFIVLLPKKILIAN 245

Query: 192 -------------VVIKLDLEKAFDKVDWDFLDAVLQAEGFGCVWRKWIRGCISSANYSI 251
                        VV K+D EKA+D V WDFLD VL+ +GF   WRKW+RGC+ S +Y+I
Sbjct: 246 EIVDEKRRSGEEGVVFKIDFEKAYDHVKWDFLDHVLKKKGFSSKWRKWMRGCLLSVSYAI 305

Query: 252 IIN-------------------GRPRGENPKLSSFWQPVIERIQRKLHSWKYMYISK--- 311
           ++N                     P GENP    FW PVIERI R+L  +   ++ K   
Sbjct: 306 LVNDVLSRMLLKAEERSLLKGFNLPLGENPTACGFWDPVIERISRRLDGFLLQWLQKLRG 365

Query: 312 ---------GGRQPMEKND--------------------FAKFYNAAGICWWPKPIMGGS 347
                     G+ P+                            Y      W    ++  S
Sbjct: 366 CKGTFFGQGLGKIPLRNRALLGKWLWRYLRESTALWHQVILSIYGTHSNGWDANTLVRWS 425

[Close]

BLAST of Lag0035538 vs. NCBI nr
Match: RVW70261.1 (Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera])

HSP 1 Score: 146.7 bits (369), Expect = 3.6e-31
Identity = 106/433 (24.48%), Postives = 151/433 (34.87%), Query Frame = 0


Query: 67   KDIEEEFIDFYKKLYTKDSTSRFLPTNLEWGRINASQSSALETPFTEEEVYSAVQSLGTS 126
            K I+EE + +++KLYT  S   +    L+W  I+   +  LE+PFTEEE++ A+  +   
Sbjct: 568  KSIKEEILRYFEKLYTSPSGESWRVEGLDWSPISGESAFRLESPFTEEEIFKAIFQMDMD 627

Query: 127  KSPGPDGFTAEFFKNSWTLLSMTLWQFC-------------------------------- 186
            K+P PDGFT   F++ W ++   L +                                  
Sbjct: 628  KAPWPDGFTIAVFQDCWEVIKEDLVKVFAEFHRNGIINQSTNASFIVLLPKKNMSRRISD 687

Query: 187  ------------------------------------------------------------ 246
                                                                        
Sbjct: 688  FRPISLITSLYKIIAKVLAGRIRGVLHETIHSTQGAFVQGRQILDAVLIANEIVDEKRRS 747

Query: 247  ----VVIKLDLEKAFDKVDWDFLDAVLQAEGFGCVWRKWIRGCISSANYSIIINGR---- 306
                VV K+D EKA+D V WDFLD VL+ +GFG  WRKW+RGC+SS ++++++NG     
Sbjct: 748  GEEGVVFKIDFEKAYDHVSWDFLDHVLEMKGFGLRWRKWMRGCLSSVSFAVLVNGNAKGW 807

Query: 307  ------------------------------------------------------------ 325
                                                                        
Sbjct: 808  VKASRGLRQGDPLSPFLFTIVADVLSRMLLKAEERNVLEGFRNHLSRLAEMLDCKASGWP 867

[Close]

BLAST of Lag0035538 vs. NCBI nr
Match: RVX09324.1 (LINE-1 retrotransposable element ORF2 protein [Vitis vinifera])

HSP 1 Score: 144.8 bits (364), Expect = 1.4e-30
Identity = 126/569 (22.14%), Postives = 192/569 (33.74%), Query Frame = 0


Query: 2   ELLRWN-KSIKVSSENLQSLISQLKALDNLEDCDSLSSDQMSQRTHLREQIEDITAREHT 61
           +L  WN  +     E  + +++ L  +D +E   +L+SD + +RT  R ++ED+  +E  
Sbjct: 175 KLKEWNIMTFGDLKERKKLILTDLSRIDLIEQEGNLNSDLVLERTLRRRELEDVLLKEEV 234

Query: 62  YWRQRSK------------------------------------------DIEEEFIDFYK 121
            WRQ+S+                                          DI EE ++F+ 
Sbjct: 235 QWRQKSRVKWIKEGDCNSKFFHRVATGRRSRKFIKSLISERGETLNNIEDISEEIVNFFG 294

Query: 122 KLYTKDSTSRFLPTNLEWGRINASQSSALETPFTEEEVYSAVQSLGTSKSPGPDGFTAEF 181
            LY+K     +    ++W  I+      L+ PFTEEEV  AV  L   K+PGP+GFT   
Sbjct: 295 NLYSKPVGESWRVEGIDWVPISGESGVWLDRPFTEEEVRMAVFQLNKEKAPGPNGFTIAV 354

Query: 182 FKNSWTLLSMTLWQFC-------------------------------------------- 241
           ++  W ++   L +                                              
Sbjct: 355 YQECWDVIKEDLMRVFLEFHTNGVLSGRLRKVLHETISGSQGAFIEGRHILDAVLIANEV 414

Query: 242 -----------VVIKLDLEKAFDKVDWDFLDAVLQAEGFGCVWRKWIRGCISSANYSIII 301
                      +V K+D EKA+D VDW FLD VLQ +G    WR WIRGC+SS++++I++
Sbjct: 415 VDEKRRSGEEGIVFKIDFEKAYDHVDWGFLDHVLQRKGSSRKWRSWIRGCLSSSSFAILV 474

Query: 302 NGR--------------------------------------------------------- 347
           NG                                                          
Sbjct: 475 NGNAKGWVKASRGLRQGDPLSPFLFTLVADVLSRMLFRAEETGFTEASMEHLQNLKIILL 534

[Close]

BLAST of Lag0035538 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 53.1 bits (126), Expect = 7.1e-06
Identity = 28/79 (35.44%), Postives = 46/79 (58.23%), Query Frame = 0


Query: 67  KDIEEEFIDFYKKLYTK-----DSTSRFLPTNLEWGRINASQSSALETPFTEEEVYSAVQ 126
           ++I+     FYK+LY+      D   +FL    +  ++N  Q   L +P + +E+ + + 
Sbjct: 412 EEIQNTIRSFYKRLYSTKLENLDEMDKFL-DRYQVPKLNQDQVDHLNSPISPKEIEAVIN 471

Query: 127 SLGTSKSPGPDGFTAEFFK 141
           SL T KSPGPDGF+AEF++
Sbjct: 472 SLPTKKSPGPDGFSAEFYQ 489

[Close]

BLAST of Lag0035538 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.6e-05
Identity = 29/78 (37.18%), Postives = 42/78 (53.85%), Query Frame = 0


Query: 68  DIEEEFIDFYKKLYTK-----DSTSRFLPTNLEWGRINASQSSALETPFTEEEVYSAVQS 127
           +I+    ++YK LY       +    FL T     R+N  +  +L  P T  E+ + + S
Sbjct: 406 EIQTTIREYYKHLYANKLENLEEMDTFLDT-YTLPRLNQEEVESLNRPITGSEIVAIINS 465

Query: 128 LGTSKSPGPDGFTAEFFK 141
           L T KSPGPDGFTAEF++
Sbjct: 466 LPTKKSPGPDGFTAEFYQ 482

[Close]

BLAST of Lag0035538 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 2.3e-04
Identity = 27/77 (35.06%), Postives = 45/77 (58.44%), Query Frame = 0


Query: 68  DIEEEFIDFYKKLYT-KDSTSRFLPTNLE---WGRINASQSSALETPFTEEEVYSAVQSL 127
           +I++   ++YKKLY+ K    + +   LE     R++  +   L  P +  E+ S +Q+L
Sbjct: 405 EIQKILNEYYKKLYSHKYENLKEIDQYLEACHLPRLSQKEVEMLNRPISSSEIASTIQNL 464

Query: 128 GTSKSPGPDGFTAEFFK 141
              KSPGPDGFT+EF++
Sbjct: 465 PKKKSPGPDGFTSEFYQ 481

[Close]

BLAST of Lag0035538 vs. ExPASy TrEMBL
Match: A0A438CT04 (Uridylate kinase OS=Vitis vinifera OX=29760 GN=pyrH_6 PE=4 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 5.2e-36
Identity = 104/317 (32.81%), Postives = 154/317 (48.58%), Query Frame = 0


Query: 2   ELLRWNK-SIKVSSENLQSLISQLKALDNLEDCDSLSSDQMSQRTHLREQIEDITAREHT 61
           +L  WNK +     E    ++S L  +D +E   +L+ D +S+RT  R ++ED+  +E  
Sbjct: 608 KLKDWNKVAFGDLRERKNLILSNLGRIDLIEQEGNLNLDLLSERTLRRRKLEDLLLKEEV 667

Query: 62  YWRQRSK------------DIE---EEFIDFYKKLYTKDSTSRFLPTNLEWGRINASQSS 121
           +WRQ+S+            +IE   EE ++F+ KLY+K   + +    L+W  I+   ++
Sbjct: 668 HWRQKSRVKWIKEGDCNSNNIEVIFEEILNFFGKLYSKPEGASWRVEGLDWVPISRESAA 727

Query: 122 ALETPFTEEEVYSAVQSLGTSKSPGPDGFTAEFFKNSWTLLSMTLW-------------Q 181
            L+ PF EEEV  AV  L   K+ GPDGFT   +++ W ++   L              Q
Sbjct: 728 WLDRPFIEEEVRLAVFQLNKEKALGPDGFTIAVYQDCWNVIKENLMRVFLEFHTNGIINQ 787

Query: 182 FC-------------------------------------------VVIKLDLEKAFDKVD 241
            C                                           VV K+D EKA+D VD
Sbjct: 788 RCLRKVLHETIFGSQGAFVEGRHILDAVLIANEVVDEKRRSGEEGVVFKIDFEKAYDYVD 847

Query: 242 WDFLDAVLQAEGFGCVWRKWIRGCISSANYSIIINGR----PRGENPKLSSFWQPVIERI 243
           W FLD VL+ + F   WR WIRGC+SS++++I++NG     P G NPK   FW PV+ERI
Sbjct: 848 WGFLDHVLERKEFSPKWRSWIRGCLSSSSFAILVNGNAKGFPLGGNPKTIDFWDPVVERI 907

[Close]

BLAST of Lag0035538 vs. ExPASy TrEMBL
Match: A0A438GXK3 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=CK203_051555 PE=4 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 6.4e-34
Identity = 90/281 (32.03%), Postives = 134/281 (47.69%), Query Frame = 0


Query: 25   KALDNLEDCDSLSSDQMSQRTHLREQIEDITAREHTYWRQRSKDIEEEFIDFYKKLYTKD 84
            K +D +E   +L+ D +S+RT  R+++ED+  +E        + I EE ++F+  LY+K 
Sbjct: 846  KRIDRIEQEGNLNLDLVSERTLRRKELEDLLLKEEGETLNNIEVISEEIVNFFGNLYSKP 905

Query: 85   STSRFLPTNLEWGRINASQSSALETPFTEEEVYSAVQSLGTSKSPGPDGFTAEFFKNSWT 144
                +    ++W  I+   +  L+ PF+EEEV  AV  L   K+P PDGFT   ++  W 
Sbjct: 906  EGDSWKIEGIDWAPISEESAIWLDRPFSEEEVRMAVFQLNKEKAPDPDGFTIAVYQECWD 965

Query: 145  LLSMTL-------------------------------------------WQFCVVIKLDL 204
            ++   L                                            +  VV K+D 
Sbjct: 966  VIKEDLNEGRLRKVLHETIFGSQGAFVEGRQILDAVLIANEVVDEKRRSGEEGVVFKIDF 1025

Query: 205  EKAFDKVDWDFLDAVLQAEGFGCVWRKWIRGCISSANYSIIINGR--------------- 243
            EKA+D V+W FLD VLQ +GF   WR W+RGC+SS++++I++NG                
Sbjct: 1026 EKAYDHVEWGFLDHVLQRKGFSQKWRSWMRGCLSSSSFAILVNGNAKGWVKPLESVKWPL 1085

[Close]

BLAST of Lag0035538 vs. ExPASy TrEMBL
Match: A0A438EVW8 (Putative ribonuclease H protein OS=Vitis vinifera OX=29760 GN=VvCHDh000004_264 PE=4 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 1.6e-32
Identity = 108/437 (24.71%), Postives = 171/437 (39.13%), Query Frame = 0


Query: 12  VSSENLQSLISQLKALDNLEDCDSLSSDQMSQRTHLREQIEDITAREHTYWRQ------- 71
           V  E  ++++ ++  +D  E   +LSSD + QR    +       R    + +       
Sbjct: 66  VGKERKKTILDEIANIDANEQERALSSDLVVQRALFHKVANGRRNRNFIKFLENESGLVL 125

Query: 72  -RSKDIEEEFIDFYKKLYTKDSTSRFLPTNLEWGRINASQSSALETPFTEEEVYSAVQSL 131
             S+ I EE + ++KKLY+      +    ++W  I+   +S L++ F+E E+Y+ +  L
Sbjct: 126 DNSESITEEILLYFKKLYSSPPGESWRVEGIDWSPISEESASRLDSLFSEVEIYNVIFQL 185

Query: 132 GTSKSPGPDGFTAEFFKNSWTLLSMTLWQFC----------------------------- 191
              K+ GPDGFT   F++ W ++   L +                               
Sbjct: 186 NRDKASGPDGFTIAMFQDCWDVIKDDLVRVFAEFHNSGIINQNTNASFIVLLPKKILIAN 245

Query: 192 -------------VVIKLDLEKAFDKVDWDFLDAVLQAEGFGCVWRKWIRGCISSANYSI 251
                        VV K+D EKA+D V WDFLD VL+ +GF   WRKW+RGC+ S +Y+I
Sbjct: 246 EIVDEKRRSGEEGVVFKIDFEKAYDHVKWDFLDHVLKKKGFSSKWRKWMRGCLLSVSYAI 305

Query: 252 IIN-------------------GRPRGENPKLSSFWQPVIERIQRKLHSWKYMYISK--- 311
           ++N                     P GENP    FW PVIERI R+L  +   ++ K   
Sbjct: 306 LVNDVLSRMLLKAEERSLLKGFNLPLGENPTACGFWDPVIERISRRLDGFLLQWLQKLRG 365

Query: 312 ---------GGRQPMEKND--------------------FAKFYNAAGICWWPKPIMGGS 347
                     G+ P+                            Y      W    ++  S
Sbjct: 366 CKGTFFGQGLGKIPLRNRALLGKWLWRYLRESTALWHQVILSIYGTHSNGWDANTLVRWS 425

[Close]

BLAST of Lag0035538 vs. ExPASy TrEMBL
Match: A0A438JK46 (LINE-1 retrotransposable element ORF2 protein OS=Vitis vinifera OX=29760 GN=LORF2_207 PE=4 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 6.6e-31
Identity = 126/569 (22.14%), Postives = 192/569 (33.74%), Query Frame = 0


Query: 2   ELLRWN-KSIKVSSENLQSLISQLKALDNLEDCDSLSSDQMSQRTHLREQIEDITAREHT 61
           +L  WN  +     E  + +++ L  +D +E   +L+SD + +RT  R ++ED+  +E  
Sbjct: 175 KLKEWNIMTFGDLKERKKLILTDLSRIDLIEQEGNLNSDLVLERTLRRRELEDVLLKEEV 234

Query: 62  YWRQRSK------------------------------------------DIEEEFIDFYK 121
            WRQ+S+                                          DI EE ++F+ 
Sbjct: 235 QWRQKSRVKWIKEGDCNSKFFHRVATGRRSRKFIKSLISERGETLNNIEDISEEIVNFFG 294

Query: 122 KLYTKDSTSRFLPTNLEWGRINASQSSALETPFTEEEVYSAVQSLGTSKSPGPDGFTAEF 181
            LY+K     +    ++W  I+      L+ PFTEEEV  AV  L   K+PGP+GFT   
Sbjct: 295 NLYSKPVGESWRVEGIDWVPISGESGVWLDRPFTEEEVRMAVFQLNKEKAPGPNGFTIAV 354

Query: 182 FKNSWTLLSMTLWQFC-------------------------------------------- 241
           ++  W ++   L +                                              
Sbjct: 355 YQECWDVIKEDLMRVFLEFHTNGVLSGRLRKVLHETISGSQGAFIEGRHILDAVLIANEV 414

Query: 242 -----------VVIKLDLEKAFDKVDWDFLDAVLQAEGFGCVWRKWIRGCISSANYSIII 301
                      +V K+D EKA+D VDW FLD VLQ +G    WR WIRGC+SS++++I++
Sbjct: 415 VDEKRRSGEEGIVFKIDFEKAYDHVDWGFLDHVLQRKGSSRKWRSWIRGCLSSSSFAILV 474

Query: 302 NGR--------------------------------------------------------- 347
           NG                                                          
Sbjct: 475 NGNAKGWVKASRGLRQGDPLSPFLFTLVADVLSRMLFRAEETGFTEASMEHLQNLKIILL 534

[Close]

BLAST of Lag0035538 vs. ExPASy TrEMBL
Match: A0A438J9S5 (Transposon TX1 uncharacterized 149 kDa protein OS=Vitis vinifera OX=29760 GN=YTX2_690 PE=4 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 9.5e-30
Identity = 127/590 (21.53%), Postives = 197/590 (33.39%), Query Frame = 0


Query: 1    MELLRWN-KSIKVSSENLQSLISQLKALDNLEDCDSLSSDQMSQRTHLREQIEDITAREH 60
            ++L  WN  +     E  + +++ L  +D +E   +L+ D + +RT  R+++ED+  +E 
Sbjct: 609  LKLKEWNIMTFGDLKERKKLILTDLSRIDLIEQEGNLNPDLVLERTLRRKELEDVLLKEE 668

Query: 61   TYWRQRSK------------------------------------------DIEEEFIDFY 120
              WRQ+S+                                          DI EE ++F+
Sbjct: 669  VQWRQKSRVKWIKEGDCNSKFFHRVATCRRSRKFIKSLISERGETLNNIEDISEEIVNFF 728

Query: 121  KKLYTKDSTSRFLPTNLEWGRINASQSSALETPFTEEEVYSAVQSLGTSKSPGPDGFTAE 180
              LY+K     +    ++W  I+      L+ PFTEEEV  AV  L   K+PGPDGFT  
Sbjct: 729  GNLYSKPVGESWRVEGIDWVPISGESGVWLDRPFTEEEVRMAVFQLNKEKAPGPDGFTIA 788

Query: 181  FFKNSWTLLSMTLWQFC------------------------------------------- 240
             ++  W ++   L +                                             
Sbjct: 789  VYQECWDVIKEDLMRVFLEFHTNGVINQSTNATFIALVPKKSQSFKISDYRPISLVTSLY 848

Query: 241  -----------------------------------------------------VVIKLDL 300
                                                                 +V K+D 
Sbjct: 849  KIIAKVLSGRLRKVLHETISGSQGAFVEGRHILDAVLIANEVVDERRRSGEEGIVFKIDF 908

Query: 301  EKAFDKVDWDFLDAVLQAEGFGCVWRKWIRGCISSANYSIIINGR--------------- 347
            EKA+D VDW FLD VLQ +GF   WR WIRGC+SS++++I++NG                
Sbjct: 909  EKAYDHVDWGFLDHVLQRKGFSQKWRSWIRGCLSSSSFAILVNGMQRVGLRLLEFADDTI 968

[Close]

BLAST of Lag0035538 vs. TAIR 10
Match: AT1G43760.1 (DNAse I-like superfamily protein )

HSP 1 Score: 43.5 bits (101), Expect = 4.0e-04
Identity = 24/64 (37.50%), Postives = 38/64 (59.38%), Query Frame = 0


Query: 80  LYTKDSTSRFLPTNLEWGRINASQSSALETPFTEEEVYSAVQSLGTSKSPGPDGFTAEFF 139
           + T DS  R    ++   R N + +S L    +++E+ +AV ++  +K+PGPD FTAEFF
Sbjct: 502 ILTPDSVQRI--KDIHPFRCNDTLASRLSALPSDKEITAAVFAMPRNKAPGPDSFTAEFF 561

Query: 140 KNSW 144
             SW
Sbjct: 562 WESW 563

The following BLAST results are available for this feature:

BLAST of Lag0035538 vs. NCBI nr


Match Name	E-value	Identity	Description
RVW26335.1	1.1e-35	32.81	Uridylate kinase [Vitis vinifera]	[more]
RVW76917.1	1.3e-33	32.03	hypothetical protein CK203_051555 [Vitis vinifera]	[more]
RVW51835.1	3.3e-32	24.71	putative ribonuclease H protein [Vitis vinifera]	[more]
RVW70261.1	3.6e-31	24.48	Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera]	[more]
RVX09324.1	1.4e-30	22.14	LINE-1 retrotransposable element ORF2 protein [Vitis vinifera]	[more]

BLAST of Lag0035538 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P11369	7.1e-06	35.44	LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE...	[more]
O00370	1.6e-05	37.18	LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1	[more]
P08548	2.3e-04	35.06	LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1	[more]

BLAST of Lag0035538 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A438CT04	5.2e-36	32.81	Uridylate kinase OS=Vitis vinifera OX=29760 GN=pyrH_6 PE=4 SV=1	[more]
A0A438GXK3	6.4e-34	32.03	Uncharacterized protein OS=Vitis vinifera OX=29760 GN=CK203_051555 PE=4 SV=1	[more]
A0A438EVW8	1.6e-32	24.71	Putative ribonuclease H protein OS=Vitis vinifera OX=29760 GN=VvCHDh000004_264 P...	[more]
A0A438JK46	6.6e-31	22.14	LINE-1 retrotransposable element ORF2 protein OS=Vitis vinifera OX=29760 GN=LORF...	[more]
A0A438J9S5	9.5e-30	21.53	Transposon TX1 uncharacterized 149 kDa protein OS=Vitis vinifera OX=29760 GN=YTX...	[more]

BLAST of Lag0035538 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G43760.1	4.0e-04	37.50	DNAse I-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000477	Reverse transcriptase domain	PFAM	PF00078	RVT_1	coord: 152..207 e-value: 2.1E-8 score: 34.0
None	No IPR available	PANTHER	PTHR33116	REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED	coord: 9..147
None	No IPR available	PANTHER	PTHR33116:SF38	OS01G0158850 PROTEIN	coord: 154..207
None	No IPR available	PANTHER	PTHR33116	REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED	coord: 154..207
None	No IPR available	PANTHER	PTHR33116:SF38	OS01G0158850 PROTEIN	coord: 9..147

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0035538.1	Lag0035538.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044237	cellular metabolic process
cellular_component	GO:0110165	cellular anatomical entity
molecular_function	GO:0003824	catalytic activity