Lag0035516 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0035516
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionDNA-directed DNA polymerase
Locationchr3: 23184942 .. 23187806 (+)
RNA-Seq ExpressionLag0035516
SyntenyLag0035516
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTCGGCGGTCGGTGAGCACCGCTCGCCAGCGGCGGTAATTTTTTTTTTTATTATTTTTTTATCTTCTTCTATCCTTTCTCCAAAGAGCGAACATATTCTACAAGTAAGAACTGCTTACTTTTGAACTTAAAAAAAAAAGAACTACTTACTTTTTCTTATCCAACGTCAACCATAACTTTAATTCGAACAGTTCAAACCTAAATGCAGAAGAGTTCCTTAGAATCAGATATAAAACACTTAATTAAATCCAAAAAGAGATTAGAGCAAGTAATTAGCCACTAATCCTTGAGGCCCTTTGGGTTGTGGATTGTTGTCGAATGTTGCCTCAGGGGAGAAATCACGTTGCTTAGAGTTCCAAGTTGTCAAGAACAGATCTGGCTATCGGAGATGGAGGTTCGTCCTCGCCAATTCGTGGTCGTCGAACAGATGAGCTTGCGCCCCCGTGGGTCCGTCGTTGGAGGTTCCGTCACACTACAAACTCTTGCACTGTGGCTCGTCGCCAGGAGAGTTGGTTGCGTGTGCGTAGCGGTTCGTGATAGGTGCTCGCGCCAGAGAAGAAGACCGCTCGAATTCGCCGTCGCGTGGGTTGCTCCAAACACATCGCTGCCGGTCTGGGTCACAGGTCGGAGGAGCAGCTGCTAACGTCGAAGGAGGAGGAGACTGGCCTGAGTTGGGCTGGAGACAAAGGTGGGGGGAGGGAAGAACAAGCCACCGCCGGCGGTTTGCGATTTCGCTGGAAAATCGGGGAGGGAGCTCCCTCCTTTGTTTTTCCGGCGAAAAACGGGGAGGGAGGGAGCTTCCTCCCTACTTGGTTTCTTTTTTTAAAAAAAAAAACTGGAGAATATGGGAGAAGAGAGGGAGAAGAAAAGAAATAAAGAAGAAGAAGAAGGAAAAAAAAAAAAGTCACCGGCGGCGAGCGGTGGTCGCCGGGCGCCAGCCATGGAGGAAGATTGAAGAAGAAGAAGAAGTGGTGGAAGGAAGAGAATGAGGAAAAGAAAATAATAAAAATAAATCAAATAAATTAAAAATAATAATATTTTAATTATTTTAATATATTTTTATTTTAAAAATTAAAAATTAATTTTTTAATTAAAATAATAGTGCCACGTCCGCAGAGGGTTAGATTTAACAGTAAAATGGATGGCAGGGACCAAAATGCTCCAAAACTAAAACCTCAAGGACTAATTTGTTGAGTTTGAAACCTCAGGGACCAAAATGAAACCTAACTCAAACCTTAGGGACTAAAAGTGTATTTTTCCCATTAATATATTGTTTTAATTTAAATTTAATTAAATTTGATTTAATTGAATTGCATTAAAACTATAGGTTATGTGGTATTATTTATATAAAATTGTTTTATATAAATGTTATTAAATTAAGTGTTGATTTAATTAAATCAAATTAATTAATAAGTTATTTATTAATTAATTAATTTAAAATTAACTATCAGTTAACTTAACTGATAGAAAATTTCACTCCCACTAACCTATTCACGATTGGTAGAGAGTTTTTTTAGGGTTATTGGTCCAAGTTTGACGGATAATCCCTACGCCCTCTCTAACGAATAGGCTCTCTCTCACAAGTGAATACTCTGAGTTTTCTCTCCTTCCAAGAATCACAGAGAATCCTTCTTGCTCCCAAGAACTCCCTAGAATTTCTTTGGTTCCCACAAACCACTCAACTCCAATCCTTAGGAGAATACCAGTGTGATTGGCTGGTGGTGTTCGTGGTGTTTTTCCAGCAAGGAAAAGGAGATTTTTTCTGCTGTTTTTTGGTGAGAATTCAAGGGATTTTGGTGAAACAATCAAGAACTTCAATTTGAGTAAGTGTTCTTAAATCTCTAGATCATTAGGATCTCTTTTTAGATTAGATCTAGAAATTGTTTAATGATTACAACATGTTTTATTTGGTTTTTACTGCTGTGAATGTTTTGCTGTCGTATTGGTATCCCAATCGACGTTTAATTCGAAAAAGGTCTCTGACGTCTTCCGCTGCCTTGGGCTTTTAACCCTACACGAACAACCTGTGAAGGATTAACTTATGAATCATGGTTAAATTAAATTCAAAATTATTGAATTTTTGGAGGTGGAGTGCATGGTGACTAAACCCATGCACTCCCCTCATGAAAGCCACCCAAATTTCCACTTCTTCTTGGCTGATTGAGTGTCAATCTGTAGGGCTTTTCTTTTTCATGAATACCTCTATAAAAGGGAAAAAGATTGCTGAAGAAAGAGATCTTGAAACCTACTGAATTGCTGCCAAAAATGAAGCTCACCATACCCACCTCAATAAGCTTGTTTTTCATGCATTTTCACTTCAATTCCATCACTTGAGGATCCCACAATCTGTTCCATAGGTTTGAGGATAGCAAGGAAGATCCTTTGGTGGTTCGTGACTCGTTGGTGAAGAAAAACAAGAAGGAAATCCAATCAGTTTCTCCATTGAAACAAGGTTGGTAAACTGTCTCTGTGTTTCCTCTTATGAACTGCATGCAATGTTTGATCAAATTGAGCTTCTTATGTGGCTTAGATCCAACATTGTTTCCGTATTTTAGATATTAACACTTTCAAAAACACCTCAAAGCACACAATTTAAGCACAAAACTAGCTAAAATTAATAGGAAATAAATAGCATATTTGCATGGTATCAGAGATCCTGACAAAGAAGAAGAGATTAGGAGAGTTCGAGAGTGTGTCTCTTATTGAGGAATGTAGTGCTATTCTTAAGAATGGGCTACCTCCCAAGGCTAAGGATCCAGGATCATTCACTATTCCTATCTCTAGAGGAGAAAATGAGTTGGGGAGAGCACTTTGTGATTTAGGCGCAAGCATAAACCTTATGCCTCTCTCAATTTATCAATAG

mRNA sequence

ATGGTCGGCGGTCGGGGAGAAATCACGTTGCTTAGAGTTCCAAGTTGTCAAGAACAGATCTGGCTATCGGAGATGGAGGTTCGTCCTCGCCAATTCGTGGTCGTCGAACAGATGAGCTTGCGCCCCCGTGGGTCCGTCGTTGGAGGTTCCGTCACACTACAAACTCTTGCACTGTGGCTCGTCGCCAGGAGAGTTGGTTGCGTGTGCGTAGCGGTTCGTGATAGGTCGGAGGAGCAGCTGCTAACGTCGAAGGAGGAGGAGACTGGCCTGAGTTGGGCTGGAGACAAAGGTGGGGGGAGGGAAGAACAAGCCACCGCCGGCGGTTTGCGATTTCGCTGGAAAATCGGGGAGGGAGCTCCCTCCTTTGTTTTTCCGGCGAAAAACGGGGAGGGAGGGAGCTTCCTCCCTACTTGGTTTGAGGATAGCAAGGAAGATCCTTTGGTGGTTCGTGACTCGTTGGTGAAGAAAAACAAGAAGGAAATCCAATCAGTTTCTCCATTGAAACAAGAGATCCTGACAAAGAAGAAGAGATTAGGAGAGTTCGAGAGTGTGTCTCTTATTGAGGAATGTAGTGCTATTCTTAAGAATGGGCTACCTCCCAAGGCTAAGGATCCAGGATCATTCACTATTCCTATCTCTAGAGGAGAAAATGAGTTGGGGAGAGCACTTTGTGATTTAGGCGCAAGCATAAACCTTATGCCTCTCTCAATTTATCAATAG

Coding sequence (CDS)

ATGGTCGGCGGTCGGGGAGAAATCACGTTGCTTAGAGTTCCAAGTTGTCAAGAACAGATCTGGCTATCGGAGATGGAGGTTCGTCCTCGCCAATTCGTGGTCGTCGAACAGATGAGCTTGCGCCCCCGTGGGTCCGTCGTTGGAGGTTCCGTCACACTACAAACTCTTGCACTGTGGCTCGTCGCCAGGAGAGTTGGTTGCGTGTGCGTAGCGGTTCGTGATAGGTCGGAGGAGCAGCTGCTAACGTCGAAGGAGGAGGAGACTGGCCTGAGTTGGGCTGGAGACAAAGGTGGGGGGAGGGAAGAACAAGCCACCGCCGGCGGTTTGCGATTTCGCTGGAAAATCGGGGAGGGAGCTCCCTCCTTTGTTTTTCCGGCGAAAAACGGGGAGGGAGGGAGCTTCCTCCCTACTTGGTTTGAGGATAGCAAGGAAGATCCTTTGGTGGTTCGTGACTCGTTGGTGAAGAAAAACAAGAAGGAAATCCAATCAGTTTCTCCATTGAAACAAGAGATCCTGACAAAGAAGAAGAGATTAGGAGAGTTCGAGAGTGTGTCTCTTATTGAGGAATGTAGTGCTATTCTTAAGAATGGGCTACCTCCCAAGGCTAAGGATCCAGGATCATTCACTATTCCTATCTCTAGAGGAGAAAATGAGTTGGGGAGAGCACTTTGTGATTTAGGCGCAAGCATAAACCTTATGCCTCTCTCAATTTATCAATAG

Protein sequence

MVGGRGEITLLRVPSCQEQIWLSEMEVRPRQFVVVEQMSLRPRGSVVGGSVTLQTLALWLVARRVGCVCVAVRDRSEEQLLTSKEEETGLSWAGDKGGGREEQATAGGLRFRWKIGEGAPSFVFPAKNGEGGSFLPTWFEDSKEDPLVVRDSLVKKNKKEIQSVSPLKQEILTKKKRLGEFESVSLIEECSAILKNGLPPKAKDPGSFTIPISRGENELGRALCDLGASINLMPLSIYQ
Homology
[Close]
BLAST of Lag0035516 vs. NCBI nr
Match: XP_024028757.1 (uncharacterized protein LOC112093792 [Morus notabilis])

HSP 1 Score: 113.6 bits (283), Expect = 2.3e-21
Identity = 54/81 (66.67%), Postives = 68/81 (83.95%), Query Frame = 0 

Query: 159 KEIQSVSPLKQEILTKKKRLGEFESVSLIEECSAILKNGLPPKAKDPGSFTIPISRGENE 218
           +++ S     ++ILTKK+RLGEFE+V+L EECSAILKN LPPK KDPGSFTIP S G+  
Sbjct: 361 EQMPSYVKFMKDILTKKRRLGEFETVALTEECSAILKNRLPPKLKDPGSFTIPCSIGDQY 420

Query: 219 LGRALCDLGASINLMPLSIYQ 240
           +G+ALCDLGASINLMP+SI++
Sbjct: 421 IGKALCDLGASINLMPMSIFR 441

[Close]
BLAST of Lag0035516 vs. NCBI nr
Match: XP_024022362.1 (uncharacterized protein LOC112091881 [Morus notabilis])

HSP 1 Score: 110.5 bits (275), Expect = 2.0e-20
Identity = 53/81 (65.43%), Postives = 67/81 (82.72%), Query Frame = 0 

Query: 159 KEIQSVSPLKQEILTKKKRLGEFESVSLIEECSAILKNGLPPKAKDPGSFTIPISRGENE 218
           +++ S     + IL+KK+RLGEFE+V+L EECSAILKN LPPK KDPGSFTIP S G+  
Sbjct: 131 EQMPSYVKFMKVILSKKRRLGEFETVALTEECSAILKNRLPPKLKDPGSFTIPCSIGDQY 190

Query: 219 LGRALCDLGASINLMPLSIYQ 240
           +G+ALCDLGASINLMP+SI++
Sbjct: 191 IGKALCDLGASINLMPMSIFR 211

[Close]
BLAST of Lag0035516 vs. NCBI nr
Match: XP_024028414.1 (uncharacterized protein LOC112093694 [Morus notabilis])

HSP 1 Score: 108.6 bits (270), Expect = 7.5e-20
Identity = 51/81 (62.96%), Postives = 66/81 (81.48%), Query Frame = 0 

Query: 159 KEIQSVSPLKQEILTKKKRLGEFESVSLIEECSAILKNGLPPKAKDPGSFTIPISRGENE 218
           +++ S +   ++++TKK RLGEF++V+L EECSAIL+N LPPK KDPGSFTIP S G   
Sbjct: 234 EQMPSYAKFMKDLMTKKHRLGEFKTVALTEECSAILQNKLPPKLKDPGSFTIPCSIGNQY 293

Query: 219 LGRALCDLGASINLMPLSIYQ 240
            GRALCDLGASINLMP+SI++
Sbjct: 294 FGRALCDLGASINLMPMSIFK 314

[Close]
BLAST of Lag0035516 vs. NCBI nr
Match: XP_030509265.1 (uncharacterized protein LOC115723943 [Cannabis sativa])

HSP 1 Score: 108.2 bits (269), Expect = 9.8e-20
Identity = 50/71 (70.42%), Postives = 63/71 (88.73%), Query Frame = 0 

Query: 169 QEILTKKKRLGEFESVSLIEECSAILKNGLPPKAKDPGSFTIPISRGENELGRALCDLGA 228
           ++ILTKK+RLGEFE+V+L E CSA+LK+ +PPK KDPGSFTIP S G  ++GRALCDLGA
Sbjct: 385 KDILTKKRRLGEFETVALTEGCSAMLKSKIPPKLKDPGSFTIPCSIGGRDVGRALCDLGA 444

Query: 229 SINLMPLSIYQ 240
           SINLMP+SI++
Sbjct: 445 SINLMPMSIFK 455

[Close]
BLAST of Lag0035516 vs. NCBI nr
Match: XP_030502183.1 (uncharacterized protein LOC115717351 [Cannabis sativa])

HSP 1 Score: 108.2 bits (269), Expect = 9.8e-20
Identity = 50/71 (70.42%), Postives = 63/71 (88.73%), Query Frame = 0 

Query: 169 QEILTKKKRLGEFESVSLIEECSAILKNGLPPKAKDPGSFTIPISRGENELGRALCDLGA 228
           ++ILTKK+RLGEFE+V+L E CSA+LK+ +PPK KDPGSFTIP S G  ++GRALCDLGA
Sbjct: 110 KDILTKKRRLGEFETVALTEGCSAMLKSKIPPKLKDPGSFTIPCSIGGRDVGRALCDLGA 169

Query: 229 SINLMPLSIYQ 240
           SINLMP+SI++
Sbjct: 170 SINLMPMSIFK 180

[Close]
BLAST of Lag0035516 vs. ExPASy TrEMBL
Match: A0A2G9HP96 (DNA-directed DNA polymerase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_07975 PE=4 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 8.1e-20
Identity = 56/106 (52.83%), Postives = 74/106 (69.81%), Query Frame = 0 

Query: 138 WFEDSKEDPLVVRDSLVK----KNKKEIQSVSPLKQEILTKKKRLGEFESVSLIEECSAI 197
           WFE   ED +      +     K  +++ S     ++IL+KK+RLG++E+V+L EECSAI
Sbjct: 98  WFEYLPEDSITTWKLHINIPFAKALEQMPSYVKFMKDILSKKRRLGDYETVALTEECSAI 157

Query: 198 LKNGLPPKAKDPGSFTIPISRGENELGRALCDLGASINLMPLSIYQ 240
           ++N LPPK KDPGSFTIP + G +  GRALCDLGASINLMP SIY+
Sbjct: 158 IQNKLPPKLKDPGSFTIPCTIGTHFSGRALCDLGASINLMPYSIYR 203

[Close]
BLAST of Lag0035516 vs. ExPASy TrEMBL
Match: A0A2G9HWL8 (DNA-directed DNA polymerase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_05423 PE=4 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 1.4e-19
Identity = 55/98 (56.12%), Postives = 72/98 (73.47%), Query Frame = 0 

Query: 142 SKEDPLVVRDSLVKKNKKEIQSVSPLKQEILTKKKRLGEFESVSLIEECSAILKNGLPPK 201
           S+E    V   L  K  +++ S     ++IL+KK+RLG++E+V+L EECSAI++N LPPK
Sbjct: 420 SEEKGKEVETPLEVKALEQMPSYVKFMKDILSKKRRLGDYETVALTEECSAIIQNKLPPK 479

Query: 202 AKDPGSFTIPISRGENELGRALCDLGASINLMPLSIYQ 240
            KDPGSFTIP + G +  GRALCDLGASINLMP SIY+
Sbjct: 480 LKDPGSFTIPCTIGTHFSGRALCDLGASINLMPYSIYR 517

[Close]
BLAST of Lag0035516 vs. ExPASy TrEMBL
Match: A0A2G9HH15 (Reverse transcriptase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_10756 PE=4 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 2.4e-19
Identity = 54/98 (55.10%), Postives = 72/98 (73.47%), Query Frame = 0 

Query: 142 SKEDPLVVRDSLVKKNKKEIQSVSPLKQEILTKKKRLGEFESVSLIEECSAILKNGLPPK 201
           S+E    +   L  K  +++ S     ++IL+KK+RLG++E+V+L EECSAI++N LPPK
Sbjct: 524 SEEKGKEIEAPLEVKALEQMPSYVKFMKDILSKKRRLGDYETVALTEECSAIIQNKLPPK 583

Query: 202 AKDPGSFTIPISRGENELGRALCDLGASINLMPLSIYQ 240
            KDPGSFTIP + G +  GRALCDLGASINLMP SIY+
Sbjct: 584 LKDPGSFTIPCTIGTHFSGRALCDLGASINLMPYSIYR 621

[Close]
BLAST of Lag0035516 vs. ExPASy TrEMBL
Match: A0A2G9I766 (DNA-directed DNA polymerase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_01653 PE=4 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 3.1e-19
Identity = 58/120 (48.33%), Postives = 77/120 (64.17%), Query Frame = 0 

Query: 120 PSFVFPAKNGEGGSFLPTWFEDSKEDPLVVRDSLVKKNKKEIQSVSPLKQEILTKKKRLG 179
           PS   P    +G   +    E   E PL V      K  +++ S     ++IL+KK+RLG
Sbjct: 181 PSNTKPNPRQDGKEVISEEKEKEVESPLEV------KALEQMSSYVKFMKDILSKKRRLG 240

Query: 180 EFESVSLIEECSAILKNGLPPKAKDPGSFTIPISRGENELGRALCDLGASINLMPLSIYQ 239
           ++E+V+L EECSAI++N LPPK KDP SFTIP + G +  GRALCDLGASINL+P SIY+
Sbjct: 241 DYETVALTEECSAIIQNKLPPKLKDPRSFTIPCTTGTHFSGRALCDLGASINLIPYSIYR 294

[Close]
BLAST of Lag0035516 vs. ExPASy TrEMBL
Match: A0A2G9HYA0 (Reverse transcriptase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_04802 PE=4 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 4.0e-19
Identity = 50/81 (61.73%), Postives = 66/81 (81.48%), Query Frame = 0 

Query: 159 KEIQSVSPLKQEILTKKKRLGEFESVSLIEECSAILKNGLPPKAKDPGSFTIPISRGENE 218
           +++ S     ++IL+KK+RLG++E+V+L EECSAI++N LPPK KDPGSFTIP + G + 
Sbjct: 347 EQMPSYVKFMKDILSKKRRLGDYETVALTEECSAIIQNKLPPKLKDPGSFTIPCTIGTHF 406

Query: 219 LGRALCDLGASINLMPLSIYQ 240
            GRALCDLGASINLMP SIY+
Sbjct: 407 SGRALCDLGASINLMPYSIYR 427

The following BLAST results are available for this feature:
BLAST of Lag0035516 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_024028757.12.3e-2166.67uncharacterized protein LOC112093792 [Morus notabilis][more]
XP_024022362.12.0e-2065.43uncharacterized protein LOC112091881 [Morus notabilis][more]
XP_024028414.17.5e-2062.96uncharacterized protein LOC112093694 [Morus notabilis][more]
XP_030509265.19.8e-2070.42uncharacterized protein LOC115723943 [Cannabis sativa][more]
XP_030502183.19.8e-2070.42uncharacterized protein LOC115717351 [Cannabis sativa][more]
BLAST of Lag0035516 vs. NCBI nr
Match NameE-valueIdentityDescription
BLAST of Lag0035516 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A2G9HP968.1e-2052.83DNA-directed DNA polymerase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_079... [more]
A0A2G9HWL81.4e-1956.12DNA-directed DNA polymerase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_054... [more]
A0A2G9HH152.4e-1955.10Reverse transcriptase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_10756 PE=... [more]
A0A2G9I7663.1e-1948.33DNA-directed DNA polymerase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_016... [more]
A0A2G9HYA04.0e-1961.73Reverse transcriptase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_04802 PE=... [more]
BLAST of Lag0035516 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR33067FAMILY NOT NAMEDcoord: 159..238
NoneNo IPR availablePANTHERPTHR33067:SF13ASPARTYL PROTEASEcoord: 159..238

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0035516.1Lag0035516.1mRNA