Lag0035509 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0035509
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionProtein kinase domain-containing protein
Locationchr3: 23019544 .. 23020653 (+)
RNA-Seq ExpressionLag0035509
SyntenyLag0035509
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCGACATGATTGTGAACAGCCAGTACGAAATCCTGGAGGAGATCGGTCGAGGAAGATTCGGAACCATCACTCGCTGCTTTTGCCCCGTTTCCAACAAGTTCTTCGCCTGCAAAACCATCGCCAAGAACGATCTCCTCGACGAAACTGACAAAGAATGCCTCGACAAGGAGCCCAAAATCATGGCCCTTCTCCCTCCCCATCCCAACATTCTCCAGCTAATCGACGTTCTCGAGAACGACGATTATCTCTGTTTAATCTCCGACCTCTGCGACTCCATTTCTCTCTACGATCGGATTCTTCGTTGTCCCTTTTCCGAATCCGAAGCCGCTGTCGTTATGAAGCAGCTTCTCGAAGCGCTCAGCCACTGTCATTTCCACGGCGTCGTTCATCGCGATGTGAAGCCCGACAATGTGCTCTTTGATTCCAGGAACAATTTGAAACTCATCGATTTCGGATCGGCCGAGTGGTGTGAGAAGGACGGATTTATGTTCGGTGTCGTCGGGACGCCTTATTATGTTGCGCCTGAGGTTTTGAGAGGATCCGAGTACGATCGGAAGGTCGATGTGTGGAGCGCCGGTGTGATTCTCTACACGATGTTGGCCGGATTTCCACCGTTCTACGGCGAGTCTGCGGCGGAGGTCTTCGAGGCGGTTCTCAGAGGAAATCTGAGATTTCCGACTAGGGTTTTCAGGTCGGTTTCTTCGGCGGCTAAGGACTTGATGAAGAAGATGATCTGTCGAGATGTTTCCAGAAGATTCTCTGCAGAACAAGCTCTCAGTAAGTCAAATTTCTTCCTCTAATTCAACAATGAGTTTGTGTTCTCTTTGATCTGAACTGTTGATTTCTCTTTCCGATTCCATATCTGTCTGCAAATCTAGAAGAAACCCCAAGCCCAGTATAAATTCATTACTGATTTGAGATGCTGGGACTTGATTTCTGTGATTCTTGAGATTTGGGGTCTGTTTATTTCATTTCTTGTTTGGTTACTGAGAAAATTTCTGTATGAGCATGGAAAATCTGTCTTATGGGAATTGGAAACTGCTGTGTTTATTACAGGACACCCATGGATTTTGAGTGGAGGAGCAGGAACAGGTTCAGTGGAGTAA

mRNA sequence

ATGGGCGACATGATTGTGAACAGCCAGTACGAAATCCTGGAGGAGATCGGTCGAGGAAGATTCGGAACCATCACTCGCTGCTTTTGCCCCGTTTCCAACAAGTTCTTCGCCTGCAAAACCATCGCCAAGAACGATCTCCTCGACGAAACTGACAAAGAATGCCTCGACAAGGAGCCCAAAATCATGGCCCTTCTCCCTCCCCATCCCAACATTCTCCAGCTAATCGACGTTCTCGAGAACGACGATTATCTCTGTTTAATCTCCGACCTCTGCGACTCCATTTCTCTCTACGATCGGATTCTTCGTTGTCCCTTTTCCGAATCCGAAGCCGCTGTCGTTATGAAGCAGCTTCTCGAAGCGCTCAGCCACTGTCATTTCCACGGCGTCGTTCATCGCGATGTGAAGCCCGACAATGTGCTCTTTGATTCCAGGAACAATTTGAAACTCATCGATTTCGGATCGGCCGAGTGGTGTGAGAAGGACGGATTTATGTTCGGTGTCGTCGGGACGCCTTATTATGTTGCGCCTGAGGTTTTGAGAGGATCCGAGTACGATCGGAAGGTCGATGTGTGGAGCGCCGGTGTGATTCTCTACACGATGTTGGCCGGATTTCCACCGTTCTACGGCGAGTCTGCGGCGGAGGTCTTCGAGGCGGTTCTCAGAGGAAATCTGAGATTTCCGACTAGGGTTTTCAGGTCGGTTTCTTCGGCGGCTAAGGACTTGATGAAGAAGATGATCTGTCGAGATGTTTCCAGAAGATTCTCTGCAGAACAAGCTCTCAGACACCCATGGATTTTGAGTGGAGGAGCAGGAACAGGTTCAGTGGAGTAA

Coding sequence (CDS)

ATGGGCGACATGATTGTGAACAGCCAGTACGAAATCCTGGAGGAGATCGGTCGAGGAAGATTCGGAACCATCACTCGCTGCTTTTGCCCCGTTTCCAACAAGTTCTTCGCCTGCAAAACCATCGCCAAGAACGATCTCCTCGACGAAACTGACAAAGAATGCCTCGACAAGGAGCCCAAAATCATGGCCCTTCTCCCTCCCCATCCCAACATTCTCCAGCTAATCGACGTTCTCGAGAACGACGATTATCTCTGTTTAATCTCCGACCTCTGCGACTCCATTTCTCTCTACGATCGGATTCTTCGTTGTCCCTTTTCCGAATCCGAAGCCGCTGTCGTTATGAAGCAGCTTCTCGAAGCGCTCAGCCACTGTCATTTCCACGGCGTCGTTCATCGCGATGTGAAGCCCGACAATGTGCTCTTTGATTCCAGGAACAATTTGAAACTCATCGATTTCGGATCGGCCGAGTGGTGTGAGAAGGACGGATTTATGTTCGGTGTCGTCGGGACGCCTTATTATGTTGCGCCTGAGGTTTTGAGAGGATCCGAGTACGATCGGAAGGTCGATGTGTGGAGCGCCGGTGTGATTCTCTACACGATGTTGGCCGGATTTCCACCGTTCTACGGCGAGTCTGCGGCGGAGGTCTTCGAGGCGGTTCTCAGAGGAAATCTGAGATTTCCGACTAGGGTTTTCAGGTCGGTTTCTTCGGCGGCTAAGGACTTGATGAAGAAGATGATCTGTCGAGATGTTTCCAGAAGATTCTCTGCAGAACAAGCTCTCAGACACCCATGGATTTTGAGTGGAGGAGCAGGAACAGGTTCAGTGGAGTAA

Protein sequence

MGDMIVNSQYEILEEIGRGRFGTITRCFCPVSNKFFACKTIAKNDLLDETDKECLDKEPKIMALLPPHPNILQLIDVLENDDYLCLISDLCDSISLYDRILRCPFSESEAAVVMKQLLEALSHCHFHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCEKDGFMFGVVGTPYYVAPEVLRGSEYDRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKKMICRDVSRRFSAEQALRHPWILSGGAGTGSVE
Homology
BLAST of Lag0035509 vs. NCBI nr
Match: XP_038877083.1 (phosphoenolpyruvate carboxylase kinase 1-like [Benincasa hispida])

HSP 1 Score: 537.7 bits (1384), Expect = 5.8e-149
Identity = 253/274 (92.34%), Postives = 269/274 (98.18%), Query Frame = 0

Query: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPVSNKFFACKTIAKNDLLDETDKECLDKEPK 60
           MGDMIVNSQY+ILEEIGRGRFGTITRCFCP+SNKFFACKTIAKNDL+DETDKECL+KEPK
Sbjct: 1   MGDMIVNSQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDDYLCLISDLCDSISLYDRILRCPFSESEAAVVMKQLLEA 120
           IMA+LPPHPNILQLIDVLENDDYLCLISDLCDS+SLYDRI+R PFSESEAA+V+KQLLEA
Sbjct: 61  IMAILPPHPNILQLIDVLENDDYLCLISDLCDSLSLYDRIIRRPFSESEAAIVIKQLLEA 120

Query: 121 LSHCHFHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCEKDGFMFGVVGTPYYVAPEVLR 180
           L+HCH HGVVHRDVKPDNVLFDSRNNLKLIDFGSA+WC++DGFMFGVVGTPYY+APEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSADWCDEDGFMFGVVGTPYYIAPEVLR 180

Query: 181 GSEYDRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEY RKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGAGTGS 275
           LMKKMICRDVSRRFSAEQ+LRHPWILSGG GT +
Sbjct: 241 LMKKMICRDVSRRFSAEQSLRHPWILSGGEGTAT 274

BLAST of Lag0035509 vs. NCBI nr
Match: XP_022988181.1 (phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita maxima])

HSP 1 Score: 536.2 bits (1380), Expect = 1.7e-148
Identity = 252/276 (91.30%), Postives = 269/276 (97.46%), Query Frame = 0

Query: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPVSNKFFACKTIAKNDLLDETDKECLDKEPK 60
           MGDMIVNSQYEILEEIGRGRFGTITRCFCP+SNKFFACKTIAK+DL+DETDKECL+KEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDDYLCLISDLCDSISLYDRILRCPFSESEAAVVMKQLLEA 120
           IMALLPPHPNILQLIDV ENDDYLCLIS+LCDS+SLYDRI+R PFSESEAA+VMKQLL+A
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 121 LSHCHFHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCEKDGFMFGVVGTPYYVAPEVLR 180
           L+HCH+HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWC+KDGF FGVVGTPYY+APEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 181 GSEYDRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEY +KVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFP RVFRS+SSAAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGAGTGSVE 277
           LMKKMICRDVSRRFSAEQALRHPWILSGG GT SVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEGTSSVE 276

BLAST of Lag0035509 vs. NCBI nr
Match: KAG7023891.1 (Phosphoenolpyruvate carboxylase kinase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 533.5 bits (1373), Expect = 1.1e-147
Identity = 250/276 (90.58%), Postives = 268/276 (97.10%), Query Frame = 0

Query: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPVSNKFFACKTIAKNDLLDETDKECLDKEPK 60
           MGDMIVNSQYEILEEIGRGRFGTITRCFCP+SNKFFACKTIAK+DL+DETDKECL+KEPK
Sbjct: 43  MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 102

Query: 61  IMALLPPHPNILQLIDVLENDDYLCLISDLCDSISLYDRILRCPFSESEAAVVMKQLLEA 120
           IMALLPPHPNILQLIDV ENDDYLCLIS+LCDS+SLYDRI+R PFSESEAA+VMKQLL+A
Sbjct: 103 IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 162

Query: 121 LSHCHFHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCEKDGFMFGVVGTPYYVAPEVLR 180
           L+HCH+HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWC+KDGF FGVVGTPYY+APEVLR
Sbjct: 163 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 222

Query: 181 GSEYDRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEY +KVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFP RVFRS+S AAKD
Sbjct: 223 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 282

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGAGTGSVE 277
           LMKKMICRDVSRRFSAEQALRHPWI+SGG GT SVE
Sbjct: 283 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE 318

BLAST of Lag0035509 vs. NCBI nr
Match: XP_022961225.1 (phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita moschata] >XP_022961226.1 phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita moschata])

HSP 1 Score: 533.5 bits (1373), Expect = 1.1e-147
Identity = 250/276 (90.58%), Postives = 268/276 (97.10%), Query Frame = 0

Query: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPVSNKFFACKTIAKNDLLDETDKECLDKEPK 60
           MGDMIVNSQYEILEEIGRGRFGTITRCFCP+SNKFFACKTIAK+DL+DETDKECL+KEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDDYLCLISDLCDSISLYDRILRCPFSESEAAVVMKQLLEA 120
           IMALLPPHPNILQLIDV ENDDYLCLIS+LCDS+SLYDRI+R PFSESEAA+VMKQLL+A
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 121 LSHCHFHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCEKDGFMFGVVGTPYYVAPEVLR 180
           L+HCH+HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWC+KDGF FGVVGTPYY+APEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 181 GSEYDRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEY +KVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFP RVFRS+S AAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGAGTGSVE 277
           LMKKMICRDVSRRFSAEQALRHPWI+SGG GT SVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE 276

BLAST of Lag0035509 vs. NCBI nr
Match: KAG6590287.1 (Phosphoenolpyruvate carboxylase kinase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 531.6 bits (1368), Expect = 4.1e-147
Identity = 249/276 (90.22%), Postives = 267/276 (96.74%), Query Frame = 0

Query: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPVSNKFFACKTIAKNDLLDETDKECLDKEPK 60
           MGDMIVNSQYEILEEIGRGRFGTITRCFCP+SNKFFACKTIAK+DL+DETDKECL+KEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDDYLCLISDLCDSISLYDRILRCPFSESEAAVVMKQLLEA 120
           IMALLPPHPNILQLIDV ENDDYLCLIS+LCDS+SLYDRI+R PFSESEAA+VMKQLL+A
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 121 LSHCHFHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCEKDGFMFGVVGTPYYVAPEVLR 180
           L+HCH+HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWC+KDGF FGVVGTPYY+APEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 181 GSEYDRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEY +KVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFP RVFRS+S AAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGAGTGSVE 277
           LMKKMICRDVSRRFSAEQALRHPWI+SGG GT  VE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSPVE 276

BLAST of Lag0035509 vs. ExPASy Swiss-Prot
Match: Q9SPK4 (Phosphoenolpyruvate carboxylase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PPCK1 PE=1 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 2.6e-91
Identity = 160/265 (60.38%), Postives = 202/265 (76.23%), Query Frame = 0

Query: 8   SQYEILEEIGRGRFGTITRCFCPVSNKFFACKTIAKNDLLDETDKECLDKEPKIMALLPP 67
           ++Y+I EEIGRGRFGT++R + P +  FFACKTI K  L D+ D+ CLD EPK+MALL  
Sbjct: 13  NKYQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLSDDLDRACLDNEPKLMALLSY 72

Query: 68  HPNILQLIDVLENDDYLCLISDLC-DSISLYDRILRC-PFSESEAAVVMKQLLEALSHCH 127
           HPNI+Q+ D+++ D  L +  +L   S+S+YDR++    F E + A   KQ+L+ALSHCH
Sbjct: 73  HPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCH 132

Query: 128 FHGVVHRDVKPDNVLFDSRNN-LKLIDFGSAEWCEKDGFMFGVVGTPYYVAPEVLRGSEY 187
            +GVVHRD+KP+N+L D RN+ +K+ DFGS  W  +     GVVGTPYYVAPEVL G  Y
Sbjct: 133 RYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEGETTEGVVGTPYYVAPEVLMGYSY 192

Query: 188 DRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKK 247
             KVD+WSAGV+LYTMLAG PPFYGE+A E+FEAVLRGNLRFPT++FR VSS AKD ++K
Sbjct: 193 GEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLRK 252

Query: 248 MICRDVSRRFSAEQALRHPWILSGG 270
           +IC+D SRRFSAEQALRHPWI   G
Sbjct: 253 LICKDASRRFSAEQALRHPWIQRAG 277

BLAST of Lag0035509 vs. ExPASy Swiss-Prot
Match: Q93VK0 (Phosphoenolpyruvate carboxylase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PPCK2 PE=1 SV=2)

HSP 1 Score: 331.3 bits (848), Expect = 1.1e-89
Identity = 158/268 (58.96%), Postives = 206/268 (76.87%), Query Frame = 0

Query: 6   VNSQYEILEEIGRGRFGTITRCFCPVSNKFFACKTIAKNDLLDETDKECLDKEPKIMALL 65
           + + Y++ +EIGRGRFGTITRCF P + +F+ACKTI K  L+D  D+EC++ EP+IMA+L
Sbjct: 7   LENNYQLCDEIGRGRFGTITRCFSPATKEFYACKTIDKRVLIDALDRECIETEPRIMAML 66

Query: 66  PPHPNILQLIDVLENDDYLCLISDLCD-SISLYDRILRC--PFSESEAAVVMKQLLEALS 125
           PPHPNI+++ D+ E +D L ++ +L D  +++YDR++      SESE+A   KQ+L AL+
Sbjct: 67  PPHPNIIRIFDLYETEDSLAIVMELVDPPMTIYDRLISAGGRLSESESASYAKQILSALA 126

Query: 126 HCHFHGVVHRDVKPDNVLFD-SRNNLKLIDFGSAEWCEKDGFMFGVVGTPYYVAPEVLRG 185
           HCH   VVHRDVKPDNVL D     +KL DFGSA W   +    GVVGTPYYVAPEV+ G
Sbjct: 127 HCHRCDVVHRDVKPDNVLVDLVSGGVKLCDFGSAVWLGGE-TAEGVVGTPYYVAPEVVMG 186

Query: 186 SEYDRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
            +YD KVD+WSAGV++YTMLAG PPF GE+A ++FE++LRGNLRFP + F SVSS AKDL
Sbjct: 187 RKYDEKVDIWSAGVVIYTMLAGEPPFNGETAEDIFESILRGNLRFPPKKFGSVSSEAKDL 246

Query: 246 MKKMICRDVSRRFSAEQALRHPWILSGG 270
           ++KMICRDVSRRFSAE ALRH W+++ G
Sbjct: 247 LRKMICRDVSRRFSAEDALRHSWMMNVG 273

BLAST of Lag0035509 vs. ExPASy Swiss-Prot
Match: P28582 (Calcium-dependent protein kinase OS=Daucus carota OX=4039 PE=2 SV=2)

HSP 1 Score: 221.9 bits (564), Expect = 9.2e-57
Identity = 109/268 (40.67%), Postives = 174/268 (64.93%), Query Frame = 0

Query: 6   VNSQYEILEEIGRGRFGTITRCFCPVSNKFFACKTIAKNDLLDETDKECLDKEPKIMALL 65
           +  +Y + +E+GRG+FG + +C    S + +ACK+I K  L+ + DKE + +E +I+  L
Sbjct: 77  IRGKYTLGKELGRGQFGCVYQCTENSSGQLYACKSILKRKLVSKNDKEDIKREIQILQHL 136

Query: 66  PPHPNILQLIDVLENDDYLCLISDLCDSISLYDRIL-RCPFSESEAAVVMKQLLEALSHC 125
              PNI++   V E+   + L+ +LC    L+DRI+ +  +SE  AA + +Q++  +  C
Sbjct: 137 SGQPNIVEFKGVFEDRQSVHLVMELCAGGELFDRIIAQGHYSERAAATICRQIVNVVHVC 196

Query: 126 HFHGVVHRDVKPDNVLFDSRNN---LKLIDFGSAEWCEKDGFMFGVVGTPYYVAPEVLRG 185
           HF GV+HRD+KP+N L  S++    LK  DFG + + E+      +VG+ YYVAPEVLR 
Sbjct: 197 HFMGVMHRDLKPENFLLSSKDKDAMLKATDFGLSVFIEEGKVYRNIVGSAYYVAPEVLRR 256

Query: 186 SEYDRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
           S Y +++D+WSAGVILY +L+G PPF+ E+   +F+A+L G + F +  + SVS++AKDL
Sbjct: 257 S-YGKEIDIWSAGVILYILLSGVPPFWAENEKGIFDAILEGVIDFESEPWPSVSNSAKDL 316

Query: 246 MKKMICRDVSRRFSAEQALRHPWILSGG 270
           ++KM+ +D  RR ++ Q L HPW+  GG
Sbjct: 317 VRKMLTQDPRRRITSAQVLDHPWMREGG 343

BLAST of Lag0035509 vs. ExPASy Swiss-Prot
Match: Q9C6P3 (Calcium-dependent protein kinase 33 OS=Arabidopsis thaliana OX=3702 GN=CPK33 PE=1 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 2.7e-56
Identity = 107/264 (40.53%), Postives = 173/264 (65.53%), Query Frame = 0

Query: 10  YEILEEIGRGRFGTITRCFCPVSNKFFACKTIAKNDLLDETDKECLDKEPKIMALLPPHP 69
           Y + +E+GRG+FG    C    + K FACK+I+K  L+ + DKE + +E +IM  L   P
Sbjct: 73  YTLSKELGRGQFGVTYLCTEKSTGKRFACKSISKKKLVTKGDKEDMRREIQIMQHLSGQP 132

Query: 70  NILQLIDVLENDDYLCLISDLCDSISLYDRIL-RCPFSESEAAVVMKQLLEALSHCHFHG 129
           NI++     E++  + L+ +LC    L+DRIL +  +SE  AA V +Q++  ++ CHF G
Sbjct: 133 NIVEFKGAYEDEKAVNLVMELCAGGELFDRILAKGHYSERAAASVCRQIVNVVNICHFMG 192

Query: 130 VVHRDVKPDNVLFDSRNN---LKLIDFGSAEWCEKDGFMFGVVGTPYYVAPEVLRGSEYD 189
           V+HRD+KP+N L  S++    +K  DFG + + E+      +VG+ YYVAPEVL+   Y 
Sbjct: 193 VMHRDLKPENFLLSSKDEKALIKATDFGLSVFIEEGRVYKDIVGSAYYVAPEVLK-RRYG 252

Query: 190 RKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKKM 249
           +++D+WSAG+ILY +L+G PPF+ E+   +F+A+L G + F ++ + S+S++AKDL+++M
Sbjct: 253 KEIDIWSAGIILYILLSGVPPFWAETEKGIFDAILEGEIDFESQPWPSISNSAKDLVRRM 312

Query: 250 ICRDVSRRFSAEQALRHPWILSGG 270
           + +D  RR SA + L+HPW+  GG
Sbjct: 313 LTQDPKRRISAAEVLKHPWLREGG 335

BLAST of Lag0035509 vs. ExPASy Swiss-Prot
Match: P93759 (Calcium-dependent protein kinase 14 OS=Arabidopsis thaliana OX=3702 GN=CPK14 PE=2 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 7.8e-56
Identity = 113/267 (42.32%), Postives = 169/267 (63.30%), Query Frame = 0

Query: 6   VNSQYEILEEIGRGRFGTITRCFCPVSNKFFACKTIAKNDLLDETDKECLDKEPKIMALL 65
           +  +Y++  E+GRG FG    C    + + FACK+I K  L    D E + +E +IM  +
Sbjct: 50  IKQKYKLGRELGRGEFGVTYLCTEIETGEIFACKSILKKKLKTSIDIEDVKREVEIMRQM 109

Query: 66  PPHPNILQLIDVLENDDYLCLISDLCDSISLYDRIL-RCPFSESEAAVVMKQLLEALSHC 125
           P HPNI+ L +  E+D  + L+ +LC+   L+DRI+ R  ++E  AA V+K ++E +  C
Sbjct: 110 PEHPNIVTLKETYEDDKAVHLVMELCEGGELFDRIVARGHYTERAAASVIKTIIEVVQMC 169

Query: 126 HFHGVVHRDVKPDNVLFDSRN---NLKLIDFGSAEWCEKDGFMFGVVGTPYYVAPEVLRG 185
           H HGV+HRD+KP+N LF ++    +LK IDFG + + +       +VG+PYY+APEVLR 
Sbjct: 170 HKHGVMHRDLKPENFLFANKKETASLKAIDFGLSVFFKPGERFNEIVGSPYYMAPEVLRR 229

Query: 186 SEYDRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
           S Y +++D+WSAGVILY +L G PPF+ E+   V +A+L+  + F    +  VS  AKDL
Sbjct: 230 S-YGQEIDIWSAGVILYILLCGVPPFWAETEHGVAKAILKSVIDFKRDPWPKVSDNAKDL 289

Query: 246 MKKMICRDVSRRFSAEQALRHPWILSG 269
           +KKM+  D  RR +A+Q L HPWI +G
Sbjct: 290 IKKMLHPDPRRRLTAQQVLDHPWIQNG 315

BLAST of Lag0035509 vs. ExPASy TrEMBL
Match: A0A6J1JGI4 (phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111485498 PE=3 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 8.1e-149
Identity = 252/276 (91.30%), Postives = 269/276 (97.46%), Query Frame = 0

Query: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPVSNKFFACKTIAKNDLLDETDKECLDKEPK 60
           MGDMIVNSQYEILEEIGRGRFGTITRCFCP+SNKFFACKTIAK+DL+DETDKECL+KEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDDYLCLISDLCDSISLYDRILRCPFSESEAAVVMKQLLEA 120
           IMALLPPHPNILQLIDV ENDDYLCLIS+LCDS+SLYDRI+R PFSESEAA+VMKQLL+A
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 121 LSHCHFHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCEKDGFMFGVVGTPYYVAPEVLR 180
           L+HCH+HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWC+KDGF FGVVGTPYY+APEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 181 GSEYDRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEY +KVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFP RVFRS+SSAAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGAGTGSVE 277
           LMKKMICRDVSRRFSAEQALRHPWILSGG GT SVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEGTSSVE 276

BLAST of Lag0035509 vs. ExPASy TrEMBL
Match: A0A6J1HBL3 (phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111461799 PE=3 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 5.3e-148
Identity = 250/276 (90.58%), Postives = 268/276 (97.10%), Query Frame = 0

Query: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPVSNKFFACKTIAKNDLLDETDKECLDKEPK 60
           MGDMIVNSQYEILEEIGRGRFGTITRCFCP+SNKFFACKTIAK+DL+DETDKECL+KEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDDYLCLISDLCDSISLYDRILRCPFSESEAAVVMKQLLEA 120
           IMALLPPHPNILQLIDV ENDDYLCLIS+LCDS+SLYDRI+R PFSESEAA+VMKQLL+A
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 121 LSHCHFHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCEKDGFMFGVVGTPYYVAPEVLR 180
           L+HCH+HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWC+KDGF FGVVGTPYY+APEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 181 GSEYDRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEY +KVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFP RVFRS+S AAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGAGTGSVE 277
           LMKKMICRDVSRRFSAEQALRHPWI+SGG GT SVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE 276

BLAST of Lag0035509 vs. ExPASy TrEMBL
Match: A0A5A7TYV2 (Phosphoenolpyruvate carboxylase kinase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00970 PE=3 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 4.9e-146
Identity = 252/276 (91.30%), Postives = 264/276 (95.65%), Query Frame = 0

Query: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPVSNKFFACKTIAKNDLLDETDKECLDKEPK 60
           M DMIVNSQY+ILEEIGRGRFG ITRCFCP+SNKFFACKTI+K DL+DETDKECL KEPK
Sbjct: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKTDLIDETDKECLVKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDDYLCLISDLCDSISLYDRILRCPFSESEAAVVMKQLLEA 120
           IMALLPPHPNILQLIDVLEND YLCLISDLC+ +SLYDRI+R PFSESEAAVVMKQLL+A
Sbjct: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCEPLSLYDRIIRRPFSESEAAVVMKQLLQA 120

Query: 121 LSHCHFHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCEKDGFMFGVVGTPYYVAPEVLR 180
           L+HCH HGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWC++DGFM+GVVGTPYYVAPEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180

Query: 181 GSEYDRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEY RKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGAGTGSVE 277
           LMKKMICRDVSRR SAEQALRHPWILSGG GT SVE
Sbjct: 241 LMKKMICRDVSRRLSAEQALRHPWILSGGEGTSSVE 276

BLAST of Lag0035509 vs. ExPASy TrEMBL
Match: A0A1S3CHK9 (phosphoenolpyruvate carboxylase kinase 1 OS=Cucumis melo OX=3656 GN=LOC103501000 PE=3 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 4.9e-146
Identity = 252/276 (91.30%), Postives = 264/276 (95.65%), Query Frame = 0

Query: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPVSNKFFACKTIAKNDLLDETDKECLDKEPK 60
           M DMIVNSQY+ILEEIGRGRFG ITRCFCP+SNKFFACKTI+K DL+DETDKECL KEPK
Sbjct: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKTDLIDETDKECLVKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDDYLCLISDLCDSISLYDRILRCPFSESEAAVVMKQLLEA 120
           IMALLPPHPNILQLIDVLEND YLCLISDLC+ +SLYDRI+R PFSESEAAVVMKQLL+A
Sbjct: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCEPLSLYDRIIRRPFSESEAAVVMKQLLQA 120

Query: 121 LSHCHFHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCEKDGFMFGVVGTPYYVAPEVLR 180
           L+HCH HGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWC++DGFM+GVVGTPYYVAPEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180

Query: 181 GSEYDRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEY RKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGAGTGSVE 277
           LMKKMICRDVSRR SAEQALRHPWILSGG GT SVE
Sbjct: 241 LMKKMICRDVSRRLSAEQALRHPWILSGGEGTSSVE 276

BLAST of Lag0035509 vs. ExPASy TrEMBL
Match: A0A0A0M3G7 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G690150 PE=3 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 1.4e-145
Identity = 252/276 (91.30%), Postives = 264/276 (95.65%), Query Frame = 0

Query: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPVSNKFFACKTIAKNDLLDETDKECLDKEPK 60
           M DMIVNSQY+ILEE+GRGRFG ITRCFCP+SNKFFACKTI+KNDL DETDKECL KEPK
Sbjct: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDDYLCLISDLCDSISLYDRILRCPFSESEAAVVMKQLLEA 120
           IMALLP HPNILQLIDVLEND YLCLISDLC+S+SLYDRI+R PFSESEAAVVMKQLL+A
Sbjct: 61  IMALLPSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120

Query: 121 LSHCHFHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCEKDGFMFGVVGTPYYVAPEVLR 180
           L+HCH HGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWC++DGFM+GVVGTPYYVAPEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180

Query: 181 GSEYDRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEY RKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGAGTGSVE 277
           LMKKMICRDVSRRFSAEQALRHPWILSGG  T SVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 276

BLAST of Lag0035509 vs. TAIR 10
Match: AT1G08650.1 (phosphoenolpyruvate carboxylase kinase 1 )

HSP 1 Score: 336.7 bits (862), Expect = 1.8e-92
Identity = 160/265 (60.38%), Postives = 202/265 (76.23%), Query Frame = 0

Query: 8   SQYEILEEIGRGRFGTITRCFCPVSNKFFACKTIAKNDLLDETDKECLDKEPKIMALLPP 67
           ++Y+I EEIGRGRFGT++R + P +  FFACKTI K  L D+ D+ CLD EPK+MALL  
Sbjct: 13  NKYQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLSDDLDRACLDNEPKLMALLSY 72

Query: 68  HPNILQLIDVLENDDYLCLISDLC-DSISLYDRILRC-PFSESEAAVVMKQLLEALSHCH 127
           HPNI+Q+ D+++ D  L +  +L   S+S+YDR++    F E + A   KQ+L+ALSHCH
Sbjct: 73  HPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCH 132

Query: 128 FHGVVHRDVKPDNVLFDSRNN-LKLIDFGSAEWCEKDGFMFGVVGTPYYVAPEVLRGSEY 187
            +GVVHRD+KP+N+L D RN+ +K+ DFGS  W  +     GVVGTPYYVAPEVL G  Y
Sbjct: 133 RYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEGETTEGVVGTPYYVAPEVLMGYSY 192

Query: 188 DRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKK 247
             KVD+WSAGV+LYTMLAG PPFYGE+A E+FEAVLRGNLRFPT++FR VSS AKD ++K
Sbjct: 193 GEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLRK 252

Query: 248 MICRDVSRRFSAEQALRHPWILSGG 270
           +IC+D SRRFSAEQALRHPWI   G
Sbjct: 253 LICKDASRRFSAEQALRHPWIQRAG 277

BLAST of Lag0035509 vs. TAIR 10
Match: AT3G04530.1 (phosphoenolpyruvate carboxylase kinase 2 )

HSP 1 Score: 331.3 bits (848), Expect = 7.6e-91
Identity = 158/268 (58.96%), Postives = 206/268 (76.87%), Query Frame = 0

Query: 6   VNSQYEILEEIGRGRFGTITRCFCPVSNKFFACKTIAKNDLLDETDKECLDKEPKIMALL 65
           + + Y++ +EIGRGRFGTITRCF P + +F+ACKTI K  L+D  D+EC++ EP+IMA+L
Sbjct: 7   LENNYQLCDEIGRGRFGTITRCFSPATKEFYACKTIDKRVLIDALDRECIETEPRIMAML 66

Query: 66  PPHPNILQLIDVLENDDYLCLISDLCD-SISLYDRILRC--PFSESEAAVVMKQLLEALS 125
           PPHPNI+++ D+ E +D L ++ +L D  +++YDR++      SESE+A   KQ+L AL+
Sbjct: 67  PPHPNIIRIFDLYETEDSLAIVMELVDPPMTIYDRLISAGGRLSESESASYAKQILSALA 126

Query: 126 HCHFHGVVHRDVKPDNVLFD-SRNNLKLIDFGSAEWCEKDGFMFGVVGTPYYVAPEVLRG 185
           HCH   VVHRDVKPDNVL D     +KL DFGSA W   +    GVVGTPYYVAPEV+ G
Sbjct: 127 HCHRCDVVHRDVKPDNVLVDLVSGGVKLCDFGSAVWLGGE-TAEGVVGTPYYVAPEVVMG 186

Query: 186 SEYDRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
            +YD KVD+WSAGV++YTMLAG PPF GE+A ++FE++LRGNLRFP + F SVSS AKDL
Sbjct: 187 RKYDEKVDIWSAGVVIYTMLAGEPPFNGETAEDIFESILRGNLRFPPKKFGSVSSEAKDL 246

Query: 246 MKKMICRDVSRRFSAEQALRHPWILSGG 270
           ++KMICRDVSRRFSAE ALRH W+++ G
Sbjct: 247 LRKMICRDVSRRFSAEDALRHSWMMNVG 273

BLAST of Lag0035509 vs. TAIR 10
Match: AT1G08650.2 (phosphoenolpyruvate carboxylase kinase 1 )

HSP 1 Score: 321.6 bits (823), Expect = 6.1e-88
Identity = 154/256 (60.16%), Postives = 196/256 (76.56%), Query Frame = 0

Query: 8   SQYEILEEIGRGRFGTITRCFCPVSNKFFACKTIAKNDLLDETDKECLDKEPKIMALLPP 67
           ++Y+I EEIGRGRFGT++R + P +  FFACKTI K  L D+ D+ CLD EPK+MALL  
Sbjct: 13  NKYQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLSDDLDRACLDNEPKLMALLSY 72

Query: 68  HPNILQLIDVLENDDYLCLISDLC-DSISLYDRILRC-PFSESEAAVVMKQLLEALSHCH 127
           HPNI+Q+ D+++ D  L +  +L   S+S+YDR++    F E + A   KQ+L+ALSHCH
Sbjct: 73  HPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCH 132

Query: 128 FHGVVHRDVKPDNVLFDSRNN-LKLIDFGSAEWCEKDGFMFGVVGTPYYVAPEVLRGSEY 187
            +GVVHRD+KP+N+L D RN+ +K+ DFGS  W  +     GVVGTPYYVAPEVL G  Y
Sbjct: 133 RYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEGETTEGVVGTPYYVAPEVLMGYSY 192

Query: 188 DRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKK 247
             KVD+WSAGV+LYTMLAG PPFYGE+A E+FEAVLRGNLRFPT++FR VSS AKD ++K
Sbjct: 193 GEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLRK 252

Query: 248 MICRDVSRRFSAEQAL 261
           +IC+D SRRFSAEQAL
Sbjct: 253 LICKDASRRFSAEQAL 268

BLAST of Lag0035509 vs. TAIR 10
Match: AT1G50700.1 (calcium-dependent protein kinase 33 )

HSP 1 Score: 220.3 bits (560), Expect = 1.9e-57
Identity = 107/264 (40.53%), Postives = 173/264 (65.53%), Query Frame = 0

Query: 10  YEILEEIGRGRFGTITRCFCPVSNKFFACKTIAKNDLLDETDKECLDKEPKIMALLPPHP 69
           Y + +E+GRG+FG    C    + K FACK+I+K  L+ + DKE + +E +IM  L   P
Sbjct: 73  YTLSKELGRGQFGVTYLCTEKSTGKRFACKSISKKKLVTKGDKEDMRREIQIMQHLSGQP 132

Query: 70  NILQLIDVLENDDYLCLISDLCDSISLYDRIL-RCPFSESEAAVVMKQLLEALSHCHFHG 129
           NI++     E++  + L+ +LC    L+DRIL +  +SE  AA V +Q++  ++ CHF G
Sbjct: 133 NIVEFKGAYEDEKAVNLVMELCAGGELFDRILAKGHYSERAAASVCRQIVNVVNICHFMG 192

Query: 130 VVHRDVKPDNVLFDSRNN---LKLIDFGSAEWCEKDGFMFGVVGTPYYVAPEVLRGSEYD 189
           V+HRD+KP+N L  S++    +K  DFG + + E+      +VG+ YYVAPEVL+   Y 
Sbjct: 193 VMHRDLKPENFLLSSKDEKALIKATDFGLSVFIEEGRVYKDIVGSAYYVAPEVLK-RRYG 252

Query: 190 RKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKKM 249
           +++D+WSAG+ILY +L+G PPF+ E+   +F+A+L G + F ++ + S+S++AKDL+++M
Sbjct: 253 KEIDIWSAGIILYILLSGVPPFWAETEKGIFDAILEGEIDFESQPWPSISNSAKDLVRRM 312

Query: 250 ICRDVSRRFSAEQALRHPWILSGG 270
           + +D  RR SA + L+HPW+  GG
Sbjct: 313 LTQDPKRRISAAEVLKHPWLREGG 335

BLAST of Lag0035509 vs. TAIR 10
Match: AT2G41860.2 (calcium-dependent protein kinase 14 )

HSP 1 Score: 219.2 bits (557), Expect = 4.2e-57
Identity = 113/267 (42.32%), Postives = 169/267 (63.30%), Query Frame = 0

Query: 6   VNSQYEILEEIGRGRFGTITRCFCPVSNKFFACKTIAKNDLLDETDKECLDKEPKIMALL 65
           +  +Y++  E+GRG FG    C    + + FACK+I K  L    D E + +E +IM  +
Sbjct: 50  IKQKYKLGRELGRGEFGVTYLCTEIETGEIFACKSILKKKLKTSIDIEDVKREVEIMRQM 109

Query: 66  PPHPNILQLIDVLENDDYLCLISDLCDSISLYDRIL-RCPFSESEAAVVMKQLLEALSHC 125
           P HPNI+ L +  E+D  + L+ +LC+   L+DRI+ R  ++E  AA V+K ++E +  C
Sbjct: 110 PEHPNIVTLKETYEDDKAVHLVMELCEGGELFDRIVARGHYTERAAASVIKTIIEVVQMC 169

Query: 126 HFHGVVHRDVKPDNVLFDSRN---NLKLIDFGSAEWCEKDGFMFGVVGTPYYVAPEVLRG 185
           H HGV+HRD+KP+N LF ++    +LK IDFG + + +       +VG+PYY+APEVLR 
Sbjct: 170 HKHGVMHRDLKPENFLFANKKETASLKAIDFGLSVFFKPGDRFNEIVGSPYYMAPEVLRR 229

Query: 186 SEYDRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
           S Y +++D+WSAGVILY +L G PPF+ E+   V +A+L+  + F    +  VS  AKDL
Sbjct: 230 S-YGQEIDIWSAGVILYILLCGVPPFWAETEHGVAKAILKSVIDFKRDPWPKVSDNAKDL 289

Query: 246 MKKMICRDVSRRFSAEQALRHPWILSG 269
           +KKM+  D  RR +A+Q L HPWI +G
Sbjct: 290 IKKMLHPDPRRRLTAQQVLDHPWIQNG 315

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038877083.15.8e-14992.34phosphoenolpyruvate carboxylase kinase 1-like [Benincasa hispida][more]
XP_022988181.11.7e-14891.30phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita maxima][more]
KAG7023891.11.1e-14790.58Phosphoenolpyruvate carboxylase kinase 1, partial [Cucurbita argyrosperma subsp.... [more]
XP_022961225.11.1e-14790.58phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita moschata] >XP_022961226... [more]
KAG6590287.14.1e-14790.22Phosphoenolpyruvate carboxylase kinase 1, partial [Cucurbita argyrosperma subsp.... [more]
Match NameE-valueIdentityDescription
Q9SPK42.6e-9160.38Phosphoenolpyruvate carboxylase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PPCK... [more]
Q93VK01.1e-8958.96Phosphoenolpyruvate carboxylase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PPCK... [more]
P285829.2e-5740.67Calcium-dependent protein kinase OS=Daucus carota OX=4039 PE=2 SV=2[more]
Q9C6P32.7e-5640.53Calcium-dependent protein kinase 33 OS=Arabidopsis thaliana OX=3702 GN=CPK33 PE=... [more]
P937597.8e-5642.32Calcium-dependent protein kinase 14 OS=Arabidopsis thaliana OX=3702 GN=CPK14 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1JGI48.1e-14991.30phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1HBL35.3e-14890.58phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A5A7TYV24.9e-14691.30Phosphoenolpyruvate carboxylase kinase 1 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3CHK94.9e-14691.30phosphoenolpyruvate carboxylase kinase 1 OS=Cucumis melo OX=3656 GN=LOC103501000... [more]
A0A0A0M3G71.4e-14591.30Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G690... [more]
Match NameE-valueIdentityDescription
AT1G08650.11.8e-9260.38phosphoenolpyruvate carboxylase kinase 1 [more]
AT3G04530.17.6e-9158.96phosphoenolpyruvate carboxylase kinase 2 [more]
AT1G08650.26.1e-8860.16phosphoenolpyruvate carboxylase kinase 1 [more]
AT1G50700.11.9e-5740.53calcium-dependent protein kinase 33 [more]
AT2G41860.24.2e-5742.32calcium-dependent protein kinase 14 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 10..265
e-value: 2.6E-88
score: 309.4
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 10..265
e-value: 5.2E-71
score: 239.1
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 10..265
score: 46.986374
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 4..272
e-value: 7.7E-86
score: 290.3
NoneNo IPR availablePIRSRPIRSR037921-2PIRSR037921-2coord: 10..208
e-value: 1.3E-8
score: 31.4
NoneNo IPR availablePANTHERPTHR24349SERINE/THREONINE-PROTEIN KINASEcoord: 6..270
NoneNo IPR availablePANTHERPTHR24349:SF410PHOSPHOENOLPYRUVATE CARBOXYLASE KINASE 1coord: 6..270
NoneNo IPR availableCDDcd05117STKc_CAMKcoord: 9..264
e-value: 2.04629E-120
score: 342.919
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 16..43
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 129..141
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 6..266

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0035509.1Lag0035509.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0018105 peptidyl-serine phosphorylation
biological_process GO:0046777 protein autophosphorylation
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0009931 calcium-dependent protein serine/threonine kinase activity
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0004683 calmodulin-dependent protein kinase activity
molecular_function GO:0004672 protein kinase activity