Lag0035501 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0035501
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr3: 22815283 .. 22816778 (-)
RNA-Seq ExpressionLag0035501
SyntenyLag0035501
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCACTATTGAAACGTCTTCGGGGGATGCTGAGACTCCGTGGATGGTTGGCGGTGATTTTAATGCGAACTTGTACCAGAATGAGAAGGAAGGAGGAAGGGCTAAATCAAAATCTGAACTAAACGGTTTTCGAGAGGCTGTGGACTCGTGCAGTTTGATTGATTTTGGGTTCACAGGGAGAGGTTTACGTCGTGTAATAGAAGGTAGGGTACGGGAACAGTCTGGGAGAGAATTGACAGATGCTTTGGAAACATGGCACTACAAATGCTTTTCCCACACGCTGAGGTGAAACATTTGGACTTCAGCCGATCGGATCATCACCCTATTTTGCTATCATTAACGCCAATGGTTCGAATGGTTGATGCTCAGGGGAGCAGAATTTATAGATTTGAGGAGGCCTGGTTGTTGGATCCGGGATTTATGGAGGTGGTTAAGAGCAGTTGGGGGGCAAGTCTGTCGGTTGGATCGCCGAGAGGAGTGGCAGGGGGACTGGGAAATGCCTGGAGGCAATGAGGCGTTGGGGAAGGGGGAGGTGTGGTAGGTATGGGGAGAGAATATGGGAAGCGTCTGATGAGGTTCAAAGGGCTATGGGTCGATTGAGCACCTCTGTGTCTAGTGCAGAGCTACAGGCAGTTGAGGCCAGATTGGAGGCGATATTCCTGGAGGAAGAGGTATACTAGAAACAACGCTCCAGGGAGCATTGGTTGAAATGGGGTGACAGGAATACCCGCTGGTTTCACACACATGCTTCATTTAGGAGGAAGAGGAATCTAATTCGGGGTTTGGTAGACAGTGGTGGCGTGATGAGGCATGAGCCTGGAGAGATTGTGGGTCTGGTCTCAGAGTACTTCGAGAACATCTTCACGTCTAGTTGTCCGACAGCCAAGGATATTGATGTTGTTACAGCAGGGGTGAGGAGATCAGTAACAGATGAGATGAACAGACGACTGATGAGACCTTTCCACCAGGAGGAGATTCTCCTTGCTTTGAAACAAATGCATCCTAATAAAGCTCCGGGGTCGGATGGGCTTTCAGGGCTTTCTTTAGGAAGTCATGGGAGGTGGTGGGACCGAATGTAGTGAGGTGTTGTCTGAGCATTCTGAATGAGGGGTATCTCCAGGGTCGGTCAACGAGACCATGATTGTCTTGATTCCGAAAGTCAAAAACCCCTGTAGGGTCTCAAAGTTTAGACCCATATCCCTTTGTAATGTGGTGTACAAGCTAGTCTTCAAAGCACTGGTGAACAGAATGAAAGGAATTCTGAACATGCTAATCTCCCAAAACCAGAGTGCCTTTATCCCGGGTCGATGTGTGGTGGATAATGTCATACTGGGTTATGAGTGCATCCATGCTTTGAAGAAAAGGAGGGGGAAAACTGAGTGGGCCTCACTCAAGCTTGACATGAGTAAGGCCTACGATCGGGTGGAATGGGTGTATTTGGAGCAGATTATGCTGAAAATGGGATTCGAGCAGGGATGGGTCGATTCACCGTGA

mRNA sequence

ATGGGCACTATTGAAACGTCTTCGGGGGATGCTGAGACTCCGTGGATGGTTGGCGGTGATTTTAATGCGAACTTGTACCAGAATGAGAAGGAAGGAGGAAGGGCTAAATCAAAATCTGAACTAAACGGTTTTCGAGAGGCTGTGGACTCGTGCAGTTTGATTGATTTTGGGTTCACAGGGAGAGGTTTACGTCGTGTAATAGAAGGTAGGGTACGGGAACAGTCTGGGAGAGAATTGACAGATGCTTTGGAAACATGGCACTACAAATGCTTTTCCCACACGCTGAGGGGAGCAGAATTTATAGATTTGAGGAGGCCTGGTTGTTGGATCCGGGATTTATGGAGGTGGTTAAGAGCAGTTGGGGGGCAAGTCTGTCGGTTGGATCGCCGAGAGGAGTGGCAGGGGGACTGGGAAATGCCTGGAGGCAATGAGGCGTTGGGGAAGGGGGAGAGCTACAGGCAGTTGAGGCCAGATTGGAGGCGATATTCCTGGAGGAAGAGGAGGAAGAGGAATCTAATTCGGGGTTTGGTAGACAGTGGTGGCGTGATGAGGCATGAGCCTGGAGAGATTGTGGGTCTGGTCTCAGAGTACTTCGAGAACATCTTCACGTCTAGTTGTCCGACAGCCAAGGATATTGATGTTGTTACAGCAGGGGTGAGGAGATCAGTAACAGATGAGATGAACAGACGACTGATGAGACCTTTCCACCAGGAGGAGATTCTCCTTGCTTTGAAACAAATGCATCCTAATAAAGCTCCGGGGTCGGATGGGCTTTCAGGGCTTTCTTTAGGAAGTCATGGGAGGGTCTCAAAGTTTAGACCCATATCCCTTTGTAATGTGGTGTACAAGCTAGTCTTCAAAGCACTGGTGAACAGAATGAAAGGAATTCTGAACATGCTAATCTCCCAAAACCAGAGTGCCTTTATCCCGGGTCGATGTGTGGTGGATAATGTCATACTGGGTTATGAGTGCATCCATGCTTTGAAGAAAAGGAGGGGGAAAACTGAGTGGGCCTCACTCAAGCTTGACATGAGTAAGGCCTACGATCGGGTGGAATGGGTGTATTTGGAGCAGATTATGCTGAAAATGGGATTCGAGCAGGGATGGGTCGATTCACCGTGA

Coding sequence (CDS)

ATGGGCACTATTGAAACGTCTTCGGGGGATGCTGAGACTCCGTGGATGGTTGGCGGTGATTTTAATGCGAACTTGTACCAGAATGAGAAGGAAGGAGGAAGGGCTAAATCAAAATCTGAACTAAACGGTTTTCGAGAGGCTGTGGACTCGTGCAGTTTGATTGATTTTGGGTTCACAGGGAGAGGTTTACGTCGTGTAATAGAAGGTAGGGTACGGGAACAGTCTGGGAGAGAATTGACAGATGCTTTGGAAACATGGCACTACAAATGCTTTTCCCACACGCTGAGGGGAGCAGAATTTATAGATTTGAGGAGGCCTGGTTGTTGGATCCGGGATTTATGGAGGTGGTTAAGAGCAGTTGGGGGGCAAGTCTGTCGGTTGGATCGCCGAGAGGAGTGGCAGGGGGACTGGGAAATGCCTGGAGGCAATGAGGCGTTGGGGAAGGGGGAGAGCTACAGGCAGTTGAGGCCAGATTGGAGGCGATATTCCTGGAGGAAGAGGAGGAAGAGGAATCTAATTCGGGGTTTGGTAGACAGTGGTGGCGTGATGAGGCATGAGCCTGGAGAGATTGTGGGTCTGGTCTCAGAGTACTTCGAGAACATCTTCACGTCTAGTTGTCCGACAGCCAAGGATATTGATGTTGTTACAGCAGGGGTGAGGAGATCAGTAACAGATGAGATGAACAGACGACTGATGAGACCTTTCCACCAGGAGGAGATTCTCCTTGCTTTGAAACAAATGCATCCTAATAAAGCTCCGGGGTCGGATGGGCTTTCAGGGCTTTCTTTAGGAAGTCATGGGAGGGTCTCAAAGTTTAGACCCATATCCCTTTGTAATGTGGTGTACAAGCTAGTCTTCAAAGCACTGGTGAACAGAATGAAAGGAATTCTGAACATGCTAATCTCCCAAAACCAGAGTGCCTTTATCCCGGGTCGATGTGTGGTGGATAATGTCATACTGGGTTATGAGTGCATCCATGCTTTGAAGAAAAGGAGGGGGAAAACTGAGTGGGCCTCACTCAAGCTTGACATGAGTAAGGCCTACGATCGGGTGGAATGGGTGTATTTGGAGCAGATTATGCTGAAAATGGGATTCGAGCAGGGATGGGTCGATTCACCGTGA

Protein sequence

MGTIETSSGDAETPWMVGGDFNANLYQNEKEGGRAKSKSELNGFREAVDSCSLIDFGFTGRGLRRVIEGRVREQSGRELTDALETWHYKCFSHTLRGAEFIDLRRPGCWIRDLWRWLRAVGGQVCRLDRREEWQGDWEMPGGNEALGKGESYRQLRPDWRRYSWRKRRKRNLIRGLVDSGGVMRHEPGEIVGLVSEYFENIFTSSCPTAKDIDVVTAGVRRSVTDEMNRRLMRPFHQEEILLALKQMHPNKAPGSDGLSGLSLGSHGRVSKFRPISLCNVVYKLVFKALVNRMKGILNMLISQNQSAFIPGRCVVDNVILGYECIHALKKRRGKTEWASLKLDMSKAYDRVEWVYLEQIMLKMGFEQGWVDSP
Homology
BLAST of Lag0035501 vs. NCBI nr
Match: CCA66050.1 (hypothetical protein [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 215.7 bits (548), Expect = 6.8e-52
Identity = 160/484 (33.06%), Postives = 219/484 (45.25%), Query Frame = 0

Query: 12  ETPWMVGGDFNANLYQNEKEGGRAKSKSELNGFREAVDSCSLIDFGFTG------RGLRR 71
           E P + GGDFN  L  +EKEGG ++ +  + GFR  +D CSL D  F G      RG  R
Sbjct: 130 EGPIVFGGDFNEILSYDEKEGGASRERRAIVGFRNVMDDCSLGDLRFVGQWHTWERG--R 189

Query: 72  VIEGRVREQSGRELTDA--LETWHYKCFSHTLR-------------GAEFIDLRRP-GCW 131
             E R+RE+  R +     L  +      H +R             G E +  RR  G W
Sbjct: 190 SPESRIRERLDRFIVSRSWLHLFPEAFIDHQVRYCSDHAAIVLRCLGNEGMPRRRAGGFW 249

Query: 132 IRDLW------------RWLRAVGGQVCR-----LDRREEW------------------- 191
               W             W  A GG++C          + W                   
Sbjct: 250 FETFWLLDDTCEEVVRGAWNAAEGGRICEKLGAVARELQGWSKKTFGSLRKKIEAVEKKL 309

Query: 192 ---QGD------WEMPGG-----NEALGKGESYRQLR------------PDWRRYSWRKR 251
              QG+      WE   G     +E   K E+Y  LR              +  +   +R
Sbjct: 310 HAAQGEATSIDSWERCVGLERELDELHAKNEAYWYLRSRVAEVKDGDRNTSYFHHKASQR 369

Query: 252 RKRNLIRGLVDSGGVMRHEPGEIVGLVSEYFENIFTSSCPTAKDIDVVTAGVRRSVTDEM 311
           +KRNLI G+ D GG  + E  EI  +V  YF+ IFTSS P++ D   V   V+RSVT E 
Sbjct: 370 KKRNLIHGIFDGGGRWQTEGEEIECVVERYFQEIFTSSEPSSNDFQEVLQHVKRSVTQEY 429

Query: 312 NRRLMRPFHQEEILLALKQMHPNKAPGSDGLSGL-------------------------- 371
           N  L++P+ +EEI  AL  MHP KAPG DG+  +                          
Sbjct: 430 NDILLKPYSKEEIFAALSDMHPCKAPGPDGMHAIFYQRFWHIIGDEVFNFVSSILHNYSC 489

BLAST of Lag0035501 vs. NCBI nr
Match: XP_030942013.1 (uncharacterized protein LOC115967068 [Quercus lobata])

HSP 1 Score: 208.8 bits (530), Expect = 8.3e-50
Identity = 144/452 (31.86%), Postives = 219/452 (48.45%), Query Frame = 0

Query: 4   IETSSGDAETPWMVGGDFNANLYQNEKEGGRAKSKSELNGFREAVDSCSLIDFGFTGRGL 63
           I +     + PW++ GDFN  ++ +EK G   +   ++ GFR+ +  C L+D GF G+  
Sbjct: 100 IRSLKRQCDLPWVIFGDFNEIVHSDEKLGWLDRDARQMEGFRDCLADCGLVDLGFVGQRY 159

Query: 64  RRVIEGRVREQSGRELTD---ALETW-----HYKCFSHTLRGAEF----IDLRRP----- 123
                GR+ EQ      D   A E W       K +   +  ++     + LRR      
Sbjct: 160 -TWCNGRIGEQRTLVRLDRMVANEKWLNMFREAKVYHRAMAASDHCLLNLSLRRQIQDRL 219

Query: 124 GCWIRDLWRWLRAVGGQV--------CRLDRREEWQGDWEMPGGNEALGKGESYRQLRPD 183
            C+   L  W + V G V         RL   E      E     + L K  +   LR +
Sbjct: 220 KCYQDSLQTWNKRVFGNVNKTLKLKQNRLQELEMLNLLHEPVEEIKVLKKEINEVTLREE 279

Query: 184 --WRRYS---WRK---------------RRKRNLIRGLVDSGGVMRHEPGEIVGLVSEYF 243
             W + S   W K               RR++N I+GL D+ G  + +  E+ G++  YF
Sbjct: 280 MMWNQRSRAVWVKCGDRNTKFFHETASNRRRKNRIKGLCDNEGRWKEDKEEVEGIILNYF 339

Query: 244 ENIFTSSCPTAKDIDVVTAGVRRSVTDEMNRRLMRPFHQEEILLALKQMHPNKAPGSDGL 303
           + IF++S P   +       + R V+D+MN  L++ F +EE+  ALKQMHP K+PG + +
Sbjct: 340 QEIFSTSYP--DEFGCSLGAIERRVSDDMNDDLLQEFREEELRRALKQMHPTKSPGPNSM 399

Query: 304 SGLSLGSH---------------------------------------GRVSKFRPISLCN 363
           S +   S+                                        ++S+FRPISLCN
Sbjct: 400 SPIFFQSYWDVVGPQVVDCVLNTLKTGVMPNGLNDTYICLIPKVNCPQKMSEFRPISLCN 459

Query: 364 VVYKLVFKALVNRMKGILNMLISQNQSAFIPGRCVVDNVILGYECIHAL-KKRRGKTEWA 371
           V+YK+V K L NR+K +L  +IS+ QSAF+PGR + DNV++ +E +H + +KR GK    
Sbjct: 460 VIYKIVSKVLANRLKKVLPAIISEAQSAFVPGRQITDNVLVAFETMHYINQKRMGKKGLM 519

BLAST of Lag0035501 vs. NCBI nr
Match: OMO59710.1 (reverse transcriptase [Corchorus capsularis])

HSP 1 Score: 206.1 bits (523), Expect = 5.4e-49
Identity = 156/490 (31.84%), Postives = 217/490 (44.29%), Query Frame = 0

Query: 12   ETPWMVGGDFNANLYQNEKEGGRAKSKSELNGFREAVDSCSLIDFGFTG------RGLRR 71
            E  W   GDFN  L+Q EK+GGR + ++++  FREA+D C L D G+ G      RG+  
Sbjct: 559  EKNWFCFGDFNELLWQAEKDGGRERPEAQMVAFREALDDCGLYDIGYRGNMFTWKRGMGN 618

Query: 72   VIEGRVREQSGRELTDALETWHYKCFSH----------TLRGAEFIDLRRP--------- 131
                  R   G    +    +   C +H           L   E    RR          
Sbjct: 619  NEFIHERLDRGVATFEWTSRFPTACITHLSSSVSDHSPILLNTEVKQRRRKKQSCSCKQN 678

Query: 132  -----------------GCW---------------------------------IRDLWRW 191
                              CW                                 I +L + 
Sbjct: 679  FFEAGWCKEADCEKLVVDCWEFTDGLGLLDRIVQLRDSLGKKYDQQFRSLRERIDELSKK 738

Query: 192  LRAVGGQVCRLDRREEWQGDWEMPGGNEALGKGESYRQLRPDWRRYSW------------ 251
            L  + G    +   EE +   E+   N  L + ES+      W R +W            
Sbjct: 739  LNKISGVGGHVRNSEEVELREEI---NRLLEEEESFWL---QWSRVNWLSEGDRNTSFFH 798

Query: 252  ---RKRRKRNLIRGLVDSGGVMRHEPGEIVGLVSEYFENIFTSSCPTAKDIDVVTAGVRR 311
                KRRK+N I  L    G +  +P EI  + S YF+ +F SS   +K  D +   V  
Sbjct: 799  AQASKRRKKNSIEQLEGENGRLSDDPVEIQDIASAYFKKLFISS--GSKHYDEILEAVNP 858

Query: 312  SVTDEMNRRLMRPFHQEEILLALKQMHPNKAPGSDGLS-----------GLSLGS----- 371
            S+T EMN  L+  F  EEI  ALKQ+HP KAPG DG+            G  + S     
Sbjct: 859  SITTEMNEHLLADFTAEEIFTALKQIHPTKAPGPDGMPVFFFKKFWHIVGSDVTSFCLDF 918

BLAST of Lag0035501 vs. NCBI nr
Match: XP_010686122.1 (PREDICTED: uncharacterized protein LOC104900404 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 202.6 bits (514), Expect = 5.9e-48
Identity = 151/482 (31.33%), Postives = 214/482 (44.40%), Query Frame = 0

Query: 14  PWMVGGDFNANLYQNEKEGGRAKSKSELNGFREAVDSCSLIDFGFTGRGLR----RVIEG 73
           P ++GGDFN  L  +EK+GG  + +  + GFRE +D+C L D    G+          E 
Sbjct: 321 PLVLGGDFNEILSYDEKQGGADRERRAMRGFREVIDTCGLRDLRAVGQWYTWERGDSPET 380

Query: 74  RVREQSGRELTDA--LETWHYKCFSHTLR-----GAEFIDLRRP---GCWIRDL---WRW 133
           R+RE+  R L     L+ +      H +R      A  +  + P    C +R      +W
Sbjct: 381 RIRERLDRFLVSQTWLQLFPEAVVEHLVRYKSDHAAIVLKTQAPKMKQCHMRQFKFETKW 440

Query: 134 LRAVGGQVCRLDRREEWQGD------------------WEMPGGNEALGK---------- 193
           L   G   C    RE W G                   W   G  +   K          
Sbjct: 441 LLEEG---CEATVREAWDGSVGDPIQSRLGVVARGLVGWSKAGSGDLAKKIDRVEKQLHN 500

Query: 194 --------------GESYRQL-------------------------RPDWRRYSWRKRRK 253
                         GE  ++L                            +  +   +R+K
Sbjct: 501 AQKEEISETTCKKCGELEKELDSLNAKLEAHWYMRSRVAEIKDGDRNTSYFHHKASQRKK 560

Query: 254 RNLIRGLVDSGGVMRHEPGEIVGLVSEYFENIFTSSCPTAKDIDVVTAGVRRSVTDEMNR 313
           RN I+GL D  G  R E  E+  LV +YF  IFTSS P+   +D V   V++SVT E N 
Sbjct: 561 RNRIKGLFDEHGEWREEEEELERLVQKYFREIFTSSDPSTGAMDEVLQFVKKSVTTEFND 620

Query: 314 RLMRPFHQEEILLALKQMHPNKAPGSDGLSGL---------------------------- 372
            L++P+ +EEI  ALKQMHP KAPG DGL  +                            
Sbjct: 621 ILLKPYSKEEIHEALKQMHPCKAPGPDGLHAIFYQRFWHIIGDEVFHFVSNILHSYCCPS 680

BLAST of Lag0035501 vs. NCBI nr
Match: XP_010684899.1 (PREDICTED: uncharacterized protein LOC104899410 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 202.2 bits (513), Expect = 7.8e-48
Identity = 152/452 (33.63%), Postives = 217/452 (48.01%), Query Frame = 0

Query: 12  ETPWMVGGDFNANLYQNEKEGGRAKSKSELNGFREAVDSCSLIDFGFTG------RGLRR 71
           E P + GGDFN  L  +EKEGG ++ +  + GFR  +D CSL +  F G      RG  R
Sbjct: 390 EGPVVFGGDFNEILSYDEKEGGASRERRAMVGFRNVMDDCSLRELRFVGQWHTWERG--R 449

Query: 72  VIEGRVREQSGRELTDA--LETWHYKCFSHTLR-------------GAEFIDLRRPG--- 131
             E R+RE+  R +     L  +      H +R             G E +  RR G   
Sbjct: 450 SPESRIRERLDRFIVSRSWLNIFPEAFIDHKVRYCSDHAAIVLRCLGNEGMLRRRDGGFR 509

Query: 132 ---CWIRD-------LWRWLRAVGGQVCRL--DRREEWQGDWEMPGGN------------ 191
               W+ D       +  W  A  G++C        E QG  +   G+            
Sbjct: 510 FETFWLLDDACEEVVMGAWSAAEDGRICEKLGVVARELQGWSKKSFGSLRKKIEVVEKKL 569

Query: 192 -----EALG-------------------KGESYRQLR------------PDWRRYSWRKR 251
                EA+                    K E++  +R              +  +   +R
Sbjct: 570 HEAQCEAISNDSCERCVSMEKELDELHTKNEAHLYMRSRVAEVKDGDRNTSYFHHKASQR 629

Query: 252 RKRNLIRGLVDSGGVMRHEPGEIVGLVSEYFENIFTSSCPTAKDIDVVTAGVRRSVTDEM 311
           +KRNLI G+ D GG  + E  EI  +V  YF+ IFTSS P+  D   V   V+  VT E 
Sbjct: 630 KKRNLIHGIFDGGGRWQTEGEEIECVVERYFQEIFTSSEPSFDDFQEVLQHVKVFVTQEY 689

Query: 312 NRRLMRPFHQEEILLALKQMHPNKAPGSDG---LSGLSLGSHGR----VSKFRPISLCNV 371
           N  L+ P+ +EEI  AL  MHP KAPG DG    + ++L    +    VS+FRPISLCNV
Sbjct: 690 NDVLLMPYSKEEIFAALSDMHPCKAPGPDGNVNCTNIALIPKVKPPTVVSEFRPISLCNV 749

BLAST of Lag0035501 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 6.5e-13
Identity = 67/231 (29.00%), Postives = 98/231 (42.42%), Query Frame = 0

Query: 165 RKRRKRNLIRGLVDSGGVMRHEPGEIVGLVSEYFENIFTSSCPTAKDIDVVTAGVRRSVT 224
           +K+  R  I  L    G    +P  I      +++N+F S  P + D           V+
Sbjct: 380 KKKGNRKQITCLFAEDGTPLEDPEAIRDRARSFYQNLF-SPDPISPDACEELWDGLPVVS 439

Query: 225 DEMNRRLMRPFHQEEILLALKQMHPNKAPGSDGL-------------------------- 284
           +    RL  P   +E+  AL+ M  NK+PG DGL                          
Sbjct: 440 ERRKERLETPITLDELSQALRLMPHNKSPGLDGLTIEFFQFFWDTLGPDFHRVLTEAFKK 499

Query: 285 ---------SGLSL----GSHGRVSKFRPISLCNVVYKLVFKALVNRMKGILNMLISQNQ 344
                    + LSL    G    +  +RP+SL +  YK+V KA+  R+K +L  +I  +Q
Sbjct: 500 GELPLSCRRAVLSLLPKKGDLRLIKNWRPVSLLSTDYKIVAKAISLRLKSVLAEVIHPDQ 559

Query: 345 SAFIPGRCVVDNVILGYECIHALKKRRGKTEWASLKLDMSKAYDRVEWVYL 357
           S  +PGR + DNV L  + +H    RR     A L LD  KA+DRV+  YL
Sbjct: 560 SYTVPGRTIFDNVFLIRDLLHF--ARRTGLSLAFLSLDQEKAFDRVDHQYL 607

BLAST of Lag0035501 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.0e-10
Identity = 56/243 (23.05%), Postives = 105/243 (43.21%), Query Frame = 0

Query: 165 RKRRKRNLIRGLVDSGGVMRHEPGEIVGLVSEYFENIFTSSCPTAKDID-VVTAGVRRSV 224
           RK+R ++LI  + +    +  +P EI  +++EY++ +++      K+ID  + A     +
Sbjct: 381 RKKRVKSLISSIRNGNDEITTDPSEIQKILNEYYKKLYSHKYENLKEIDQYLEACHLPRL 440

Query: 225 TDEMNRRLMRPFHQEEILLALKQMHPNKAPGSDGLSG--------------LSLGSH--- 284
           + +    L RP    EI   ++ +   K+PG DG +               L+L  +   
Sbjct: 441 SQKEVEMLNRPISSSEIASTIQNLPKKKSPGPDGFTSEFYQTFKEELVPILLNLFQNIEK 500

Query: 285 -----------------------GRVSKFRPISLCNVVYKLVFKALVNRMKGILNMLISQ 344
                                   R   +RPISL N+  K++ K L NR++  +  +I  
Sbjct: 501 EGILPNTFYEANITLIPKPGKDPTRKENYRPISLMNIDAKILNKILTNRIQQHIKKIIHH 560

Query: 345 NQSAFIPGRCVVDNVILGYECIHALKKRRGKTEWASLKLDMSKAYDRVEWVYLEQIMLKM 367
           +Q  FIPG     N+      I  + K + K +   L +D  KA+D ++  ++ + + K+
Sbjct: 561 DQVGFIPGSQGWFNIRKSINVIQHINKLKNK-DHMILSIDAEKAFDNIQHPFMIRTLKKI 620

BLAST of Lag0035501 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 5.1e-10
Identity = 52/243 (21.40%), Postives = 102/243 (41.98%), Query Frame = 0

Query: 165 RKRRKRNLIRGLVDSGGVMRHEPGEIVGLVSEYFENIFTSSCPTAKDIDV-VTAGVRRSV 224
           +K+R++N I  + +  G +  +P EI   + EY+++++ +     +++D  +       +
Sbjct: 382 KKKREKNQIDTIKNDKGDITTDPTEIQTTIREYYKHLYANKLENLEEMDTFLDTYTLPRL 441

Query: 225 TDEMNRRLMRPFHQEEILLALKQMHPNKAPGSDGLSG------------------LSLGS 284
             E    L RP    EI+  +  +   K+PG DG +                    S+  
Sbjct: 442 NQEEVESLNRPITGSEIVAIINSLPTKKSPGPDGFTAEFYQRYKEELVPFLLKLFQSIEK 501

Query: 285 HG----------------------RVSKFRPISLCNVVYKLVFKALVNRMKGILNMLISQ 344
            G                      +   FRPISL N+  K++ K L NR++  +  LI  
Sbjct: 502 EGILPNSFYEASIILIPKPGRDTTKKENFRPISLMNIDAKILNKILANRIQQHIKKLIHH 561

Query: 345 NQSAFIPGRCVVDNVILGYECIHALKKRRGKTEWASLKLDMSKAYDRVEWVYLEQIMLKM 367
           +Q  FIPG     N+      I  + + + K     + +D  KA+D+++  ++ + + K+
Sbjct: 562 DQVGFIPGMQGWFNIRKSINVIQHINRAKDKNH-VIISIDAEKAFDKIQQPFMLKTLNKL 621

BLAST of Lag0035501 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 59.3 bits (142), Expect = 1.1e-07
Identity = 50/243 (20.58%), Postives = 102/243 (41.98%), Query Frame = 0

Query: 168 RKRNLIRGLVDSGGVMRHEPGEIVGLVSEYFENIFTSSCPTAKDIDVVTAGVRRSVTDEM 227
           R + LI  + +  G +  +P EI   +  +++ ++++     +++D +   + R    ++
Sbjct: 392 RDKILINKIRNEKGDITTDPEEIQNTIRSFYKRLYSTK---LENLDEMDKFLDRYQVPKL 451

Query: 228 NR----RLMRPFHQEEILLALKQMHPNKAPGSDGLSG----------------------- 287
           N+     L  P   +EI   +  +   K+PG DG S                        
Sbjct: 452 NQDQVDHLNSPISPKEIEAVINSLPTKKSPGPDGFSAEFYQTFKEDLIPILHKLFHKIEV 511

Query: 288 ------------LSL-----GSHGRVSKFRPISLCNVVYKLVFKALVNRMKGILNMLISQ 347
                       ++L         ++  FRPISL N+  K++ K L NR++  +  +I  
Sbjct: 512 EGTLPNSFYEATITLIPKPQKDPTKIENFRPISLMNIDAKILNKILANRIQEHIKAIIHP 571

Query: 348 NQSAFIPGRCVVDNVILGYECIHALKKRRGKTEWASLKLDMSKAYDRVEWVYLEQIMLKM 367
           +Q  FIPG     N+      IH + K + K     + LD  KA+D+++  ++ +++ + 
Sbjct: 572 DQVGFIPGMQGWFNIRKSINVIHYINKLKDKNHMI-ISLDAEKAFDKIQHPFMIKVLERS 630

BLAST of Lag0035501 vs. ExPASy TrEMBL
Match: F4NCJ4 (Reverse transcriptase domain-containing protein OS=Beta vulgaris subsp. vulgaris OX=3555 PE=4 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 3.3e-52
Identity = 160/484 (33.06%), Postives = 219/484 (45.25%), Query Frame = 0

Query: 12  ETPWMVGGDFNANLYQNEKEGGRAKSKSELNGFREAVDSCSLIDFGFTG------RGLRR 71
           E P + GGDFN  L  +EKEGG ++ +  + GFR  +D CSL D  F G      RG  R
Sbjct: 130 EGPIVFGGDFNEILSYDEKEGGASRERRAIVGFRNVMDDCSLGDLRFVGQWHTWERG--R 189

Query: 72  VIEGRVREQSGRELTDA--LETWHYKCFSHTLR-------------GAEFIDLRRP-GCW 131
             E R+RE+  R +     L  +      H +R             G E +  RR  G W
Sbjct: 190 SPESRIRERLDRFIVSRSWLHLFPEAFIDHQVRYCSDHAAIVLRCLGNEGMPRRRAGGFW 249

Query: 132 IRDLW------------RWLRAVGGQVCR-----LDRREEW------------------- 191
               W             W  A GG++C          + W                   
Sbjct: 250 FETFWLLDDTCEEVVRGAWNAAEGGRICEKLGAVARELQGWSKKTFGSLRKKIEAVEKKL 309

Query: 192 ---QGD------WEMPGG-----NEALGKGESYRQLR------------PDWRRYSWRKR 251
              QG+      WE   G     +E   K E+Y  LR              +  +   +R
Sbjct: 310 HAAQGEATSIDSWERCVGLERELDELHAKNEAYWYLRSRVAEVKDGDRNTSYFHHKASQR 369

Query: 252 RKRNLIRGLVDSGGVMRHEPGEIVGLVSEYFENIFTSSCPTAKDIDVVTAGVRRSVTDEM 311
           +KRNLI G+ D GG  + E  EI  +V  YF+ IFTSS P++ D   V   V+RSVT E 
Sbjct: 370 KKRNLIHGIFDGGGRWQTEGEEIECVVERYFQEIFTSSEPSSNDFQEVLQHVKRSVTQEY 429

Query: 312 NRRLMRPFHQEEILLALKQMHPNKAPGSDGLSGL-------------------------- 371
           N  L++P+ +EEI  AL  MHP KAPG DG+  +                          
Sbjct: 430 NDILLKPYSKEEIFAALSDMHPCKAPGPDGMHAIFYQRFWHIIGDEVFNFVSSILHNYSC 489

BLAST of Lag0035501 vs. ExPASy TrEMBL
Match: A0A2N9IZB6 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS57536 PE=4 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 5.2e-50
Identity = 143/383 (37.34%), Postives = 203/383 (53.00%), Query Frame = 0

Query: 14  PWMVGGDFNANLYQNEKEGGRAKSKSELNGFREAVDSCSLIDFGFTGRGL----RRVIEG 73
           PWMV  DFN  +  +E+ G   +S +++  F+EA+    L D GF G       RR+ E 
Sbjct: 505 PWMVFSDFNEAISLDEQCGKEDRSLNQMASFKEALVDYELQDLGFKGPEFTWSNRRLGED 564

Query: 74  RVREQSGRELTDALETWHYKCFSHTLRGAEFIDLRRPGCWIRDLWRWLRAVGGQVCRLDR 133
            VR +  R +++    W+          A+   +  P      +   +R    +   L+ 
Sbjct: 565 LVRVRLDRAVSN--PAWNL-----LFPNAQVSHIIVPSSDHLRVLVEIRPNPARAQLLEM 624

Query: 134 REEWQGDWEMPGGNEALGKGESYRQLRPD--WR---RYSW---------------RKRRK 193
             +    ++  G N AL K  +    + +  WR   R +W                +R++
Sbjct: 625 ESKPMVTYDAQGVN-ALRKEITSLLAKEEVAWRQRSRVNWLADGDQNTGFFHECAAQRKR 684

Query: 194 RNLIRGLVDSGGVMRHEPGEIVGLVSEYFENIFTSSCPTAKDIDVVTAGVRRSVTDEMNR 253
            N I+GL+D     R +P E+  + + YF  +FTSS PT  D+D V   V   VT +MN 
Sbjct: 685 TNTIQGLLDREDYWRTDPHEVEQIATAYFNTLFTSSRPT--DVDDVVQVVESVVTADMNE 744

Query: 254 RLMRPFHQEEILLALKQMHPNKAPGSDGLSGLSLGSHGRVSKFRPISLCNVVYKLVFKAL 313
            L+RPF  +E+  AL QMHP+KAPG D  S  S+      S+FRPISLCNV+YK++ K L
Sbjct: 745 DLLRPFSPDEVKQALFQMHPSKAPGPDVKSPESM------SQFRPISLCNVIYKIISKIL 804

Query: 314 VNRMKGILNMLISQNQSAFIPGRCVVDNVILGYECIHALKK-RRGKTEWASLKLDMSKAY 372
           VNRMK +L  +IS++Q AF+PGR + DNVI+ +E IH LK  R GK    + KLDMSKAY
Sbjct: 805 VNRMKQVLPRVISESQRAFVPGRMITDNVIIAFEAIHHLKNLRGGKNAQLAAKLDMSKAY 864

BLAST of Lag0035501 vs. ExPASy TrEMBL
Match: A0A1R3GNW3 (Reverse transcriptase OS=Corchorus capsularis OX=210143 GN=CCACVL1_24647 PE=4 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 2.6e-49
Identity = 156/490 (31.84%), Postives = 217/490 (44.29%), Query Frame = 0

Query: 12   ETPWMVGGDFNANLYQNEKEGGRAKSKSELNGFREAVDSCSLIDFGFTG------RGLRR 71
            E  W   GDFN  L+Q EK+GGR + ++++  FREA+D C L D G+ G      RG+  
Sbjct: 559  EKNWFCFGDFNELLWQAEKDGGRERPEAQMVAFREALDDCGLYDIGYRGNMFTWKRGMGN 618

Query: 72   VIEGRVREQSGRELTDALETWHYKCFSH----------TLRGAEFIDLRRP--------- 131
                  R   G    +    +   C +H           L   E    RR          
Sbjct: 619  NEFIHERLDRGVATFEWTSRFPTACITHLSSSVSDHSPILLNTEVKQRRRKKQSCSCKQN 678

Query: 132  -----------------GCW---------------------------------IRDLWRW 191
                              CW                                 I +L + 
Sbjct: 679  FFEAGWCKEADCEKLVVDCWEFTDGLGLLDRIVQLRDSLGKKYDQQFRSLRERIDELSKK 738

Query: 192  LRAVGGQVCRLDRREEWQGDWEMPGGNEALGKGESYRQLRPDWRRYSW------------ 251
            L  + G    +   EE +   E+   N  L + ES+      W R +W            
Sbjct: 739  LNKISGVGGHVRNSEEVELREEI---NRLLEEEESFWL---QWSRVNWLSEGDRNTSFFH 798

Query: 252  ---RKRRKRNLIRGLVDSGGVMRHEPGEIVGLVSEYFENIFTSSCPTAKDIDVVTAGVRR 311
                KRRK+N I  L    G +  +P EI  + S YF+ +F SS   +K  D +   V  
Sbjct: 799  AQASKRRKKNSIEQLEGENGRLSDDPVEIQDIASAYFKKLFISS--GSKHYDEILEAVNP 858

Query: 312  SVTDEMNRRLMRPFHQEEILLALKQMHPNKAPGSDGLS-----------GLSLGS----- 371
            S+T EMN  L+  F  EEI  ALKQ+HP KAPG DG+            G  + S     
Sbjct: 859  SITTEMNEHLLADFTAEEIFTALKQIHPTKAPGPDGMPVFFFKKFWHIVGSDVTSFCLDF 918

BLAST of Lag0035501 vs. ExPASy TrEMBL
Match: A0A2N9IXL7 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS57999 PE=4 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 3.4e-49
Identity = 144/390 (36.92%), Postives = 201/390 (51.54%), Query Frame = 0

Query: 12  ETPWMVGGDFNANLYQNEKEGGRAKSKSELNGFREAVDSCSLIDFGFTG--------RGL 71
           E PWMV GDFN     +E+ G   +++++++ FREA+  C L+D GF+G        RG 
Sbjct: 515 EVPWMVLGDFNEITRLDEQVGQVDRNEAQMSSFREALLDCDLLDLGFSGPVCTWSNNRGH 574

Query: 72  RRVIEGRV-REQSGRELTD-----ALETWHYKCFSHT--------LRGAEFIDLRRPGCW 131
             ++  R+ R  +  E         +      C  H           G + +D RR   +
Sbjct: 575 TALVRARLDRAVASAEWMSLFPMATISHLAVACSDHMGLLLNTNGNSGEQRVDRRRKKLF 634

Query: 132 IRDLWRWLRAVGGQVCRLDRREEWQGDWE-MPGGNEALGKGESYRQLRPDWRRYSWRKRR 191
            R    W+R VG +       E   G W  +P G       E  +Q R            
Sbjct: 635 -RFEKAWIREVGCE-------EVIAGAWNVIPIGTAMYWVAEKIKQCR------------ 694

Query: 192 KRNLIRGLVDSGGVMRHEPGEIVGLVSEYFENIFTSSCPTAKDIDVVTAGVRRSVTDEMN 251
             NLI+         +  P EI  +   YF +IFTSS P A  I  V + V   VT  MN
Sbjct: 695 -MNLIQWSQAHDSTWQSTPAEIERIAVAYFNHIFTSSNPQA--IVEVVSEVDGVVTPGMN 754

Query: 252 RRLMRPFHQEEILLALKQMHPNKAPGSDGLSGLS-------LGSHGRVSKFRPISLCNVV 311
             L++PF +EE+  AL QM+P+KAPG DG    +       + +  ++++FRPISLCNV+
Sbjct: 755 EELLKPFVKEEVQKALFQMYPSKAPGPDGSINFTHIVLIPKVTAPEQITQFRPISLCNVL 814

Query: 312 YKLVFKALVNRMKGILNMLISQNQSAFIPGRCVVDNVILGYECIHALKK-RRGKTEWASL 371
           YK+  K LVNRMK +L  +IS++QSAF+P R + DNVI+ +E IH LK  R+G     + 
Sbjct: 815 YKIASKVLVNRMKTMLPQVISESQSAFVPERMITDNVIIAFENIHYLKNLRQGNNVQMAA 874

BLAST of Lag0035501 vs. ExPASy TrEMBL
Match: A0A2N9EV35 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS6577 PE=4 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 5.8e-49
Identity = 143/441 (32.43%), Postives = 211/441 (47.85%), Query Frame = 0

Query: 14   PWMVGGDFNANLYQNEKEGGRAKSKSELNGFREAVDSCSLIDFGFTGRGLR--RVIEGRV 73
            PW+  GDFN  + QNEK G   +S +++  FRE  + C+L+D GF+G         +G  
Sbjct: 600  PWLCFGDFNEIMVQNEKRGRYPRSLAKMCAFREVANRCNLLDMGFSGYEFTWDNNRDGMA 659

Query: 74   REQSGRELTDALETW----------------------------HYKCFSHTLRGAEFID- 133
              Q   +   A  TW                            H        +   F + 
Sbjct: 660  NVQERIDRAFASPTWSNWFPNSHVSHLPVFSSDHLPILVEVGQHVSALPRRKKPHRFEEK 719

Query: 134  -LRRPGC--WIRDLWRWLRAVGGQVCRLDRREEWQGDWEMPGGNEALGKGESYRQ-LRPD 193
             +  P C   IR LW+    VG  +  L  + +      +    E  G    ++Q +R  
Sbjct: 720  WIANPECEEIIRFLWQQEGDVGSPMYCLTEKLKRCRMGLVQWSKEKYGDEIHWKQRVRTV 779

Query: 194  WRRYSWR----------KRRKRNLIRGLVDSGGVMRHEPGEIVGLVSEYFENIFTSSCPT 253
            W +   R          +R+K N + GL+DS      +P  +  + ++YF+ +FT+S P+
Sbjct: 780  WLKVGDRNTRFFHQSATQRKKNNTVTGLMDSHDQWCTDPDGMGVIAADYFKELFTTSNPS 839

Query: 254  AKDIDVVTAGVRRSVTDEMNRRLMRPFHQEEILLALKQMHPNKAPGSDGLSGL------- 313
               ID     V R VT EMNRRL+ P++  EI  AL QMHP+K+PG DG+S +       
Sbjct: 840  R--IDNTLLAVDRVVTPEMNRRLLLPYNAVEIKRALFQMHPSKSPGPDGMSCIFFQKFWH 899

Query: 314  --------------------------------SLGSHGRVSKFRPISLCNVVYKLVFKAL 370
                                             + +  ++S +RPISLCNVVYK++ K L
Sbjct: 900  IIGCDVVQAISYVLTSGHMLKKINFTHIALIPKIKNPQQLSDYRPISLCNVVYKMISKCL 959

BLAST of Lag0035501 vs. TAIR 10
Match: AT4G20520.1 (RNA binding;RNA-directed DNA polymerases )

HSP 1 Score: 83.6 bits (205), Expect = 3.8e-16
Identity = 33/82 (40.24%), Postives = 54/82 (65.85%), Query Frame = 0

Query: 289 LVNRMKGILNMLISQNQSAFIPGRCVVDNVILGYECIHALKKRRGKTEWASLKLDMSKAY 348
           +V R+K ++  LI   Q++FIPGR   DN++   E +H++++++G   W  LKLD+ KAY
Sbjct: 1   MVERLKPLMTNLIGPAQASFIPGRVSTDNIVFVQEAVHSMRRKKGVKGWMLLKLDLEKAY 60

Query: 349 DRVEWVYLEQIMLKMGFEQGWV 371
           DR+ W YLE  ++  GF + W+
Sbjct: 61  DRIRWDYLEDTLISAGFPEVWL 82

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CCA66050.16.8e-5233.06hypothetical protein [Beta vulgaris subsp. vulgaris][more]
XP_030942013.18.3e-5031.86uncharacterized protein LOC115967068 [Quercus lobata][more]
OMO59710.15.4e-4931.84reverse transcriptase [Corchorus capsularis][more]
XP_010686122.15.9e-4831.33PREDICTED: uncharacterized protein LOC104900404 [Beta vulgaris subsp. vulgaris][more]
XP_010684899.17.8e-4833.63PREDICTED: uncharacterized protein LOC104899410 [Beta vulgaris subsp. vulgaris][more]
Match NameE-valueIdentityDescription
P143816.5e-1329.00Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV... [more]
P085481.0e-1023.05LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1[more]
O003705.1e-1021.40LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1[more]
P113691.1e-0720.58LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE... [more]
Match NameE-valueIdentityDescription
F4NCJ43.3e-5233.06Reverse transcriptase domain-containing protein OS=Beta vulgaris subsp. vulgaris... [more]
A0A2N9IZB65.2e-5037.34Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F... [more]
A0A1R3GNW32.6e-4931.84Reverse transcriptase OS=Corchorus capsularis OX=210143 GN=CCACVL1_24647 PE=4 SV... [more]
A0A2N9IXL73.4e-4936.92Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS57999 PE=4 SV=1[more]
A0A2N9EV355.8e-4932.43Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS6577 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G20520.13.8e-1640.24RNA binding;RNA-directed DNA polymerases [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 270..367
e-value: 8.8E-18
score: 64.6
NoneNo IPR availablePANTHERPTHR19446:SF440SUBFAMILY NOT NAMEDcoord: 270..371
NoneNo IPR availablePANTHERPTHR19446:SF440SUBFAMILY NOT NAMEDcoord: 135..261
NoneNo IPR availablePANTHERPTHR19446REVERSE TRANSCRIPTASEScoord: 270..371
coord: 135..261
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 212..369

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0035501.1Lag0035501.1mRNA