Lag0035312 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0035312
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionwall-associated receptor kinase-like 1
Locationchr3: 18603321 .. 18606189 (+)
RNA-Seq ExpressionLag0035312
SyntenyLag0035312
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGCTTCTTCTCCTTGCTATGCTTTTTCTCTCATCATCTTCCTTTTCTTCTTCCCTCGAGAATCCATCTGCCAGCCTACATATGTCGGAAATGGAAGCTGTTCTATCAATGATACTTCAACGCCAAAAGAGTATCTTTGCGTTGATTCTATTATTGACTCATGTGATTCATATTATCTAACCTTCAAATCTCCACCTCATTCTTATTACTTTTCTTACACCAATATCACATTAGGAAACAATATTTTGTTTAGCACTTACATTTATCCTTGTAAATGTTTGGGTGGTTCTTTTCAGAATTTACACTCTTATACTATTGAGAGTGGTGATAGTTATGAGAAAATAGTAAACGACTATTTTCAAGGGCTGACGAGCTGCTCGGCCCTAAGAACTCAAAACCATGACTTTGCTATCAATAGTTCTTCTTCCACAGGTAGACAAATTGTAGTTCCTTTGCGATGTGCTTGTCCAACCAAAGCTCAACAAGCAAATGGAACTGAGTCTTTGGCTATGTATGTGCTACAACCCAATCAAACAATTGGATCTGTTGCGAGGGACTTTAGGGTTGACAAAGAATTCGTCTTGAAAGCAAACAACCTTTCCAACTCAACCCACGCCTACCCTTTCATGCTTATTTTGGTTCCCTTGGGCAAATATGATATTTGCAGAATATTTGGTTGTGACTCTAAGTGGCGGATCCAAAACATGATGCTAGGGGTGGACAACTTTATATTGGTATAAATTTATTACAAGTTTAGTTCGTGAAATTTTAGATTTTGATTTATATAGTCACTTAATTTTTAAAAAGTGTCTAATAAGCCCCTAATATTACTTTTTTGGTCTAATTTGATTAAGTTTAATTTTGTATACAATAGGATTTTAACATATTCAAAATGTTTAAAAAATAAATGTTATATTAGACATAAAATTAAGTTGTGTCTAATAAGTTCTCAATCTATTATTTGACATTTTCTAAAATTAATGTATTTATTAGCTACAAAATTGGAAGTTTAAAGCTCAAATAGATACAAAATTAAATTGTATATATGTATAATAGATCAATTAATTTAATTTTTTCAAAATATGTTTAAGACTTATTAGATACAAAATTAAAATTTCATGAATCTATTAAACATTTTTAAAGTTTAAGAACTTATTAGACACAAAATTAAAAGTTTAATAACGCATTAAATACAAATTATAAAATTTAGGGACTAAACTTGTAATTTAACTTTCATATAAATAGAATTTTAAATTCATTGATATTTAATATTTAGAGTAAGCAATATTAATTACAAAATTAACCATGATAGAATTATCCGCGCTATGGAAGAATCAAAATTACGTTGAAGTGATCCTCTAAGATTGGGAATTATAAACTGGATAGTTAATTTTAATATTATTTTGTTGTTTAGCTAAGATAAAGCCAAGTGTAAAAAGTAGATTTAATATAATATCAATTTGATATTAATTAATTTTTTCCTACCATAACTTCAACTTGCACGTATGTCAACATTGTAGAGGTAATTACTTTAGGACATTAAAAAGTGTCTAAAGAAGTATTGTTTTTGCATTTTAATTTCTATCTTTTGGTAGTGTAATTTCAGGTGCACGCGTGTACATATACATATGAATATATCAACACATTCAAGGAATTATTACCAATCTCTCATTCTGTTTCAAACTTGTTTTTATCAATTCATTTTGAATACTAACGTCAAATATCTTTTTCTTCACTGAAGGTTTGTTTATAGTATTACTCGGTTGTTTCATTTGTGGTTGCAAAGGAAATCAAGTACTCATTAAGAAAAGAAGACGTAAGCTTGGTAAGAAAAAATTCTTCAAGCAAAATGGTGGCCTCTTGTTGCGACAAAAACTACTCCAACATAGCGAGAGGGTAAAAATATTTACACAGGAAGAACTCGAACAAGCAACGGATAATTATAGTCAAAGTAGGTTTCTTGGTCAAGGAGGATTTGGCACGGTTTACAAAGGGATACTTTGTGACAACACCCTCGTTGCAATCAAAAGGTCCAAACAAATTGAGGAAAATTGGATCGAACAATTCATTAATGAAGTTATCGTCCTCTCTCAAATTAACCACAGAAATATTGTTAGACTTCTGGGTTGTTGTTTGGAAACTGAGTTCCCTTTGCTAGTCTATGAATTTGTTTCTAACGGGACGCTTTCACACCATATTCACAGGGAAGATCCTCAAGAATCACCTTCTCTTTCTTGGGAAAATCGTCTCAATATTGCTTCTGAAGTTGCAGGAGCTATATCTTACATGCATTCTGCAGCTTCAATTCCTATTTTTCACAGAGATATTAAGTCTTTAAATATTCTTTTGGACGATAATTTTAGTGCAAAGGTTTCTGATTTTGGAACATCAAGATTTGTTTCTTGCGATCAATCTCATTTGACCACAAAAGTGTTGGGAACTTTTGGGTATATAGATCCTGAGTACTTCAGATCAAGTCAGTACACTGAAAAGAGTGATGTATATAGCTTCGGAGTCTTGTTGGTGGAGCTTTTAACAAGGAAGCAACCTGTCACTTTTCCCAAAAATGGTGGTGGGAATCTATTGGACTACTTCATTTCACTGGAAATGACTAATCGACTTGTTGAAATTCTAGATGTTACAGTTGCAAAGGAAGAAATGAGGGAGGTGGTGAACAATGTCGCGATACTTGCCATAAAATGTTTAAGATCAAATGGGAGAGAAAGGCCTACAATGAAACAAGTATATTTGGAATTGGAAGGATTGAGAAGGCCTCAAGATTGTTTAGGAATTGCTGAAGAAAGTCATTCAAGAACTTTTTCATTGCACTCATTCGAGAGTAGTGACGACGAGCTTCTAAGATAA

mRNA sequence

ATGGAGGCTTCTTCTCCTTGCTATGCTTTTTCTCTCATCATCTTCCTTTTCTTCTTCCCTCGAGAATCCATCTGCCAGCCTACATATGTCGGAAATGGAAGCTGTTCTATCAATGATACTTCAACGCCAAAAGAGTATCTTTGCGTTGATTCTATTATTGACTCATGTGATTCATATTATCTAACCTTCAAATCTCCACCTCATTCTTATTACTTTTCTTACACCAATATCACATTAGGAAACAATATTTTGTTTAGCACTTACATTTATCCTTGTAAATGTTTGGGTGGTTCTTTTCAGAATTTACACTCTTATACTATTGAGAGTGGTGATAGTTATGAGAAAATAGTAAACGACTATTTTCAAGGGCTGACGAGCTGCTCGGCCCTAAGAACTCAAAACCATGACTTTGCTATCAATAGTTCTTCTTCCACAGGTAGACAAATTGTAGTTCCTTTGCGATGTGCTTGTCCAACCAAAGCTCAACAAGCAAATGGAACTGAGTCTTTGGCTATGTATGTGCTACAACCCAATCAAACAATTGGATCTGTTGCGAGGGACTTTAGGGTTGACAAAGAATTCGTCTTGAAAGCAAACAACCTTTCCAACTCAACCCACGCCTACCCTTTCATGCTTATTTTGGTTCCCTTGGGCAAATATGATATTTGCAGAATATTTGGTTGTGACTCTAAGTGGCGGATCCAAAACATGATGCTAGGGGTGGACAACTTTATATTGGAAGAACTCGAACAAGCAACGGATAATTATAGTCAAAGTAGGTTTCTTGGTCAAGGAGGATTTGGCACGGTTTACAAAGGGATACTTTGTGACAACACCCTCGTTGCAATCAAAAGGTCCAAACAAATTGAGGAAAATTGGATCGAACAATTCATTAATGAAGTTATCGTCCTCTCTCAAATTAACCACAGAAATATTGTTAGACTTCTGGGTTGTTGTTTGGAAACTGAGTTCCCTTTGCTAGTCTATGAATTTGTTTCTAACGGGACGCTTTCACACCATATTCACAGGGAAGATCCTCAAGAATCACCTTCTCTTTCTTGGGAAAATCGTCTCAATATTGCTTCTGAAGTTGCAGGAGCTATATCTTACATGCATTCTGCAGCTTCAATTCCTATTTTTCACAGAGATATTAAGTCTTTAAATATTCTTTTGGACGATAATTTTAGTGCAAAGGTTTCTGATTTTGGAACATCAAGATTTGTTTCTTGCGATCAATCTCATTTGACCACAAAAGTGTTGGGAACTTTTGGGTATATAGATCCTGAGTACTTCAGATCAAGTCAGTACACTGAAAAGAGTGATGTATATAGCTTCGGAGTCTTGTTGGTGGAGCTTTTAACAAGGAAGCAACCTGTCACTTTTCCCAAAAATGGTGGTGGGAATCTATTGGACTACTTCATTTCACTGGAAATGACTAATCGACTTGTTGAAATTCTAGATGTTACAGTTGCAAAGGAAGAAATGAGGGAGGTGGTGAACAATGTCGCGATACTTGCCATAAAATGTTTAAGATCAAATGGGAGAGAAAGGCCTACAATGAAACAAGTATATTTGGAATTGGAAGGATTGAGAAGGCCTCAAGATTGTTTAGGAATTGCTGAAGAAAGTCATTCAAGAACTTTTTCATTGCACTCATTCGAGAGTAGTGACGACGAGCTTCTAAGATAA

Coding sequence (CDS)

ATGGAGGCTTCTTCTCCTTGCTATGCTTTTTCTCTCATCATCTTCCTTTTCTTCTTCCCTCGAGAATCCATCTGCCAGCCTACATATGTCGGAAATGGAAGCTGTTCTATCAATGATACTTCAACGCCAAAAGAGTATCTTTGCGTTGATTCTATTATTGACTCATGTGATTCATATTATCTAACCTTCAAATCTCCACCTCATTCTTATTACTTTTCTTACACCAATATCACATTAGGAAACAATATTTTGTTTAGCACTTACATTTATCCTTGTAAATGTTTGGGTGGTTCTTTTCAGAATTTACACTCTTATACTATTGAGAGTGGTGATAGTTATGAGAAAATAGTAAACGACTATTTTCAAGGGCTGACGAGCTGCTCGGCCCTAAGAACTCAAAACCATGACTTTGCTATCAATAGTTCTTCTTCCACAGGTAGACAAATTGTAGTTCCTTTGCGATGTGCTTGTCCAACCAAAGCTCAACAAGCAAATGGAACTGAGTCTTTGGCTATGTATGTGCTACAACCCAATCAAACAATTGGATCTGTTGCGAGGGACTTTAGGGTTGACAAAGAATTCGTCTTGAAAGCAAACAACCTTTCCAACTCAACCCACGCCTACCCTTTCATGCTTATTTTGGTTCCCTTGGGCAAATATGATATTTGCAGAATATTTGGTTGTGACTCTAAGTGGCGGATCCAAAACATGATGCTAGGGGTGGACAACTTTATATTGGAAGAACTCGAACAAGCAACGGATAATTATAGTCAAAGTAGGTTTCTTGGTCAAGGAGGATTTGGCACGGTTTACAAAGGGATACTTTGTGACAACACCCTCGTTGCAATCAAAAGGTCCAAACAAATTGAGGAAAATTGGATCGAACAATTCATTAATGAAGTTATCGTCCTCTCTCAAATTAACCACAGAAATATTGTTAGACTTCTGGGTTGTTGTTTGGAAACTGAGTTCCCTTTGCTAGTCTATGAATTTGTTTCTAACGGGACGCTTTCACACCATATTCACAGGGAAGATCCTCAAGAATCACCTTCTCTTTCTTGGGAAAATCGTCTCAATATTGCTTCTGAAGTTGCAGGAGCTATATCTTACATGCATTCTGCAGCTTCAATTCCTATTTTTCACAGAGATATTAAGTCTTTAAATATTCTTTTGGACGATAATTTTAGTGCAAAGGTTTCTGATTTTGGAACATCAAGATTTGTTTCTTGCGATCAATCTCATTTGACCACAAAAGTGTTGGGAACTTTTGGGTATATAGATCCTGAGTACTTCAGATCAAGTCAGTACACTGAAAAGAGTGATGTATATAGCTTCGGAGTCTTGTTGGTGGAGCTTTTAACAAGGAAGCAACCTGTCACTTTTCCCAAAAATGGTGGTGGGAATCTATTGGACTACTTCATTTCACTGGAAATGACTAATCGACTTGTTGAAATTCTAGATGTTACAGTTGCAAAGGAAGAAATGAGGGAGGTGGTGAACAATGTCGCGATACTTGCCATAAAATGTTTAAGATCAAATGGGAGAGAAAGGCCTACAATGAAACAAGTATATTTGGAATTGGAAGGATTGAGAAGGCCTCAAGATTGTTTAGGAATTGCTGAAGAAAGTCATTCAAGAACTTTTTCATTGCACTCATTCGAGAGTAGTGACGACGAGCTTCTAAGATAA

Protein sequence

MEASSPCYAFSLIIFLFFFPRESICQPTYVGNGSCSINDTSTPKEYLCVDSIIDSCDSYYLTFKSPPHSYYFSYTNITLGNNILFSTYIYPCKCLGGSFQNLHSYTIESGDSYEKIVNDYFQGLTSCSALRTQNHDFAINSSSSTGRQIVVPLRCACPTKAQQANGTESLAMYVLQPNQTIGSVARDFRVDKEFVLKANNLSNSTHAYPFMLILVPLGKYDICRIFGCDSKWRIQNMMLGVDNFILEELEQATDNYSQSRFLGQGGFGTVYKGILCDNTLVAIKRSKQIEENWIEQFINEVIVLSQINHRNIVRLLGCCLETEFPLLVYEFVSNGTLSHHIHREDPQESPSLSWENRLNIASEVAGAISYMHSAASIPIFHRDIKSLNILLDDNFSAKVSDFGTSRFVSCDQSHLTTKVLGTFGYIDPEYFRSSQYTEKSDVYSFGVLLVELLTRKQPVTFPKNGGGNLLDYFISLEMTNRLVEILDVTVAKEEMREVVNNVAILAIKCLRSNGRERPTMKQVYLELEGLRRPQDCLGIAEESHSRTFSLHSFESSDDELLR
Homology
BLAST of Lag0035312 vs. NCBI nr
Match: XP_022152076.1 (wall-associated receptor kinase-like 1 [Momordica charantia])

HSP 1 Score: 701.4 bits (1809), Expect = 6.1e-198
Identity = 380/618 (61.49%), Postives = 445/618 (72.01%), Query Frame = 0

Query: 12  LIIFLFFFPRESICQPTYVGN-GSCSINDTSTPKEYLCVDSIIDSCDSYYLTFKSPPHSY 71
           +I+F   F  +S CQ +Y+GN  SCSIN+ S PKEYLC DSI  SCDSY+  F+   H  
Sbjct: 14  IILFFSLFLYQSFCQQSYLGNETSCSINNNSGPKEYLCTDSIY-SCDSYF-AFRYELHFG 73

Query: 72  YFSYTNITLGNNILFSTYIYPCKCLGGSFQNLHSYTIESGDSYEKIVNDYFQGLTSCSAL 131
            F Y NITLG+N L    +  C CLG S+Q    YTI+SGDSYEKI +D FQGLTSCSAL
Sbjct: 74  NFYYFNITLGDNNLSIAKVL-CSCLGSSYQYNMLYTIKSGDSYEKIAHDVFQGLTSCSAL 133

Query: 132 RTQNHDFAINSSSSTGRQIVVPLRCACPTKAQQANGTESLAMYVLQPNQTIGSVARDFRV 191
           + +NHD+ + ++++ GR++ VP+RCACPTKAQ A+   SL +Y LQ N+TIGSVAR F V
Sbjct: 134 KNKNHDYPLVNTAAIGRRLEVPIRCACPTKAQIASRVHSLIVYTLQLNETIGSVARAFGV 193

Query: 192 DKEFVLKANNLSNSTHAYPFMLILVPLGKYDICRIF------------------------ 251
           +++ VL+AN LSN +H YPF  ILVPLGK D CRI+                        
Sbjct: 194 EEKSVLEANMLSNWSHIYPFTPILVPLGKDDTCRIYPYSLDCRDMSSSFSDIFSYIFLNL 253

Query: 252 -----------------GC----------------------DSKWRIQNMML----GVDN 311
                            GC                      +    +Q  +L     V  
Sbjct: 254 VLIPIAGLIGLACCFCCGCKGNQLLLKKRRRKLYKKKFFKQNGGHLLQQKLLEDSERVKI 313

Query: 312 FILEELEQATDNYSQSRFLGQGGFGTVYKGILCDNTLVAIKRSKQIEENWIEQFINEVIV 371
           +  EELEQATDNY+ SRFLGQGG+GTVYKGIL DNT+VAIKRSKQI+ +WIEQFINEVI+
Sbjct: 314 YTQEELEQATDNYNDSRFLGQGGYGTVYKGILHDNTVVAIKRSKQIDTSWIEQFINEVII 373

Query: 372 LSQINHRNIVRLLGCCLETEFPLLVYEFVSNGTLSHHIHREDPQESPSLSWENRLNIASE 431
           LSQINHRNIV+LLGCCLETEFPLLVYEFV NGTLSHHIH+E+ +ESP LSWE RL IASE
Sbjct: 374 LSQINHRNIVKLLGCCLETEFPLLVYEFVYNGTLSHHIHKENYEESPPLSWETRLRIASE 433

Query: 432 VAGAISYMHSAASIPIFHRDIKSLNILLDDNFSAKVSDFGTSRFVSCDQSHLTTKVLGTF 491
           V+GA+SYMHSAASIPI+HRDIKSLNILLD+N+SAKVSDFGTSRFVSCDQSHLTTKVLGTF
Sbjct: 434 VSGAMSYMHSAASIPIYHRDIKSLNILLDENYSAKVSDFGTSRFVSCDQSHLTTKVLGTF 493

Query: 492 GYIDPEYFRSSQYTEKSDVYSFGVLLVELLTRKQPVTFPKNGGGNLLDYFISLEMTNRLV 551
           GYIDPEYFRSSQYTEKSDVYSFGVL+VELLT K PVTF ++ G NLLDYFISLE  N+LV
Sbjct: 494 GYIDPEYFRSSQYTEKSDVYSFGVLMVELLTGKLPVTFARDDGRNLLDYFISLEKANQLV 553

Query: 552 EILDVTVAKEEMREVVNNVAILAIKCLRSNGRERPTMKQVYLELEGLRRPQDCLGIAEES 561
           EILD+ VA EE  +VVN+VA LA KCLRSNG+ERPTMKQVYLELEGLR+ +DC  I+E+ 
Sbjct: 554 EILDIIVAAEESMDVVNSVATLATKCLRSNGKERPTMKQVYLELEGLRKSRDCPDISEDV 613

BLAST of Lag0035312 vs. NCBI nr
Match: XP_022152101.1 (wall-associated receptor kinase-like 1 [Momordica charantia])

HSP 1 Score: 681.4 bits (1757), Expect = 6.6e-192
Identity = 372/611 (60.88%), Postives = 427/611 (69.89%), Query Frame = 0

Query: 11  SLIIFLFFFPRESICQPTYVGN-GSCSINDTSTPKEYLCVDSIIDSCDSYYLTFKSPPHS 70
           SL+  LFFFP +S+CQ  Y+GN  +CS+NDTST K Y+C DSI  SC SY +       S
Sbjct: 3   SLLFSLFFFPLQSLCQQPYIGNQTNCSMNDTSTSKGYICTDSIF-SCQSYLIFRSDSLLS 62

Query: 71  YYFSYTNITLGNNILFSTYIYPCKCLGGSFQNLHSYTIESGDSYEKIVNDYFQGLTSCSA 130
           Y     N TLG N LF T    C C G  +  +  YTI+ GDSYEKIVND FQG TSCS 
Sbjct: 63  YDLYLLNFTLGKNTLFRT-TADCGCWGLYYHYIAYYTIKKGDSYEKIVNDIFQGFTSCSV 122

Query: 131 LRTQNHDFAINSSSSTGRQIVVPLRCACPTKAQQANGTESLAMYVLQPNQTIGSVARDFR 190
           LR+ + D+AI+++SS GR++ VPL CACPTKAQ+A G E LA+Y+LQPN++IGSVARDF 
Sbjct: 123 LRSVSLDYAIDNTSSVGRRLQVPLGCACPTKAQKAKGVEFLAVYMLQPNESIGSVARDFG 182

Query: 191 VDKEFVLKANNLSNSTHAYPFMLILVPLGKYDICRI------------------------ 250
           V++E +L+AN LS S+  +PF  IL+PL K   CRI                        
Sbjct: 183 VEEEGILEANMLSMSSQTFPFTPILIPLNKNVTCRIDPSSYYDCNAIPPPTSNDNDYILY 242

Query: 251 ---------------FGCDSKWR------------------------IQNMML----GVD 310
                          F C  K                          +Q  +L     V 
Sbjct: 243 IVLIAIGGVIVLVSCFFCGCKGHQLLLRKRRRKLHTKKFFRQNGGLLLQQKLLENSERVK 302

Query: 311 NFILEELEQATDNYSQSRFLGQGGFGTVYKGILCDNTLVAIKRSKQIEENWIEQFINEVI 370
            F  EELEQATDNY++SRFLGQGG+GTVYKG+L DNT+VAIKRS QIE NWI+QFINEVI
Sbjct: 303 IFTHEELEQATDNYNESRFLGQGGYGTVYKGMLNDNTIVAIKRSNQIEANWIDQFINEVI 362

Query: 371 VLSQINHRNIVRLLGCCLETEFPLLVYEFVSNGTLSHHIHREDPQESPSLSWENRLNIAS 430
           +LSQINHRNIVRLLGCCLETE PLLVYEFVSNGTLSHHI+++D  ESPSLSWE RL IAS
Sbjct: 363 ILSQINHRNIVRLLGCCLETEHPLLVYEFVSNGTLSHHINQKDHGESPSLSWETRLRIAS 422

Query: 431 EVAGAISYMHSAASIPIFHRDIKSLNILLDDNFSAKVSDFGTSRFVSCDQSHLTTKVLGT 490
           EVAGAISYMHS AS  IFH+DIKSLNILLDDN+  KVSDFGTSRFVS DQSHLTTKV GT
Sbjct: 423 EVAGAISYMHSTASTQIFHKDIKSLNILLDDNYHTKVSDFGTSRFVSSDQSHLTTKVQGT 482

Query: 491 FGYIDPEYFRSSQYTEKSDVYSFGVLLVELLTRKQPVTFPKNGGGNLLDYFISLEMTNRL 550
           FGYIDPEYFRSS YTEKSDVY+FGVL+VELLT K PVTF K+ G NLL+YFISLE  N+L
Sbjct: 483 FGYIDPEYFRSSHYTEKSDVYNFGVLMVELLTGKSPVTFAKHEGRNLLEYFISLEKANQL 542

Query: 551 VEILDVTVAKEEMREVVNNVAILAIKCLRSNGRERPTMKQVYLELEGLRRPQDCLGIAEE 553
           VEILD+ VAKEE  EVVN VA L  KCLRSNG+ERPTMK VYLELE  R+PQ+CL I EE
Sbjct: 543 VEILDIAVAKEEKMEVVNAVAKLTTKCLRSNGKERPTMKHVYLELERSRKPQNCLAIIEE 602

BLAST of Lag0035312 vs. NCBI nr
Match: XP_022152099.1 (putative wall-associated receptor kinase-like 16 isoform X1 [Momordica charantia])

HSP 1 Score: 658.7 bits (1698), Expect = 4.6e-185
Identity = 366/623 (58.75%), Postives = 425/623 (68.22%), Query Frame = 0

Query: 5   SPCYAFSLII--FLFFFPRESICQPTYVG-NGSCSINDTSTPKEYLCVDSIIDSCDSYYL 64
           S C+A  LII   +  FP +S  Q +Y+G   SCSIN+ STP+E                
Sbjct: 6   SSCHALPLIILFLISLFPYQSFSQQSYLGKETSCSINNNSTPQE---------------- 65

Query: 65  TFKSPPHSYYFSYTNITLGNNILFSTYIYPCKCLGGSFQNLHSYTIESGDSYEKIVNDYF 124
                                     Y++ C C G S+Q +  YTI+SGDSYEKI  D F
Sbjct: 66  --------------------------YVWSCSCFGHSYQYIIDYTIKSGDSYEKIAYDIF 125

Query: 125 QGLTSCSALRTQNHDFAINSSSSTGRQIVVPLRCACPTKAQQANGTESLAMYVLQPNQTI 184
           QGLTSCSAL+ +N D+ +NS+++ GR++ VPLRCACPTKAQ A+  ++L +Y+LQ N+TI
Sbjct: 126 QGLTSCSALKNKNDDYPLNSTAAIGRRLEVPLRCACPTKAQNASRIDNLVVYMLQLNETI 185

Query: 185 GSVARDFRVDKEFVLKANNLSNSTHAYPFMLILVPLGKYDICRIF--------------- 244
           GSVAR F V+++ VL+AN LSN +H YPF  ILVPLGK D CRI+               
Sbjct: 186 GSVARAFGVEEKSVLEANMLSNWSHIYPFTPILVPLGKDDTCRIYPYGDCSSNSNSYLLP 245

Query: 245 -----------------------GC----------------------DSKWRIQNMML-- 304
                                  GC                      +  + +Q  +L  
Sbjct: 246 FIFLYLVTIPIGGLITLACCFFCGCKGNQLLLKKRRRKLYKKKFFKQNGGFLLQQKLLED 305

Query: 305 --GVDNFILEELEQATDNYSQSRFLGQGGFGTVYKGILCDNTLVAIKRSKQIEENWIEQF 364
              V  F  EELEQATDNYS SR LGQGG+GTVYKG+L DNT VAIKRSKQIE +WIEQF
Sbjct: 306 SERVKVFTEEELEQATDNYSDSRLLGQGGYGTVYKGMLRDNTTVAIKRSKQIETSWIEQF 365

Query: 365 INEVIVLSQINHRNIVRLLGCCLETEFPLLVYEFVSNGTLSHHIHREDPQESPSLSWENR 424
           INEVIVLSQINHRNIVRLLGCCLETEFPLLVYEFV NGTLSHHIH E+ ++SP LSWE R
Sbjct: 366 INEVIVLSQINHRNIVRLLGCCLETEFPLLVYEFVPNGTLSHHIHEENLEDSP-LSWEAR 425

Query: 425 LNIASEVAGAISYMHSAASIPIFHRDIKSLNILLDDNFSAKVSDFGTSRFVSCDQSHLTT 484
           L IASEVAGAISYMHSAASIPI+HRDIKSLNILLDDNFSAKVSDFGTSRFVSCDQSHLTT
Sbjct: 426 LRIASEVAGAISYMHSAASIPIYHRDIKSLNILLDDNFSAKVSDFGTSRFVSCDQSHLTT 485

Query: 485 KVLGTFGYIDPEYFRSSQYTEKSDVYSFGVLLVELLTRKQPVTFPKNGGGNLLDYFISLE 544
           KVLGTFGYIDPEYFRSSQYTEKSDVYSFGVL+VELLT K PV+F ++ G NLLD+FISL 
Sbjct: 486 KVLGTFGYIDPEYFRSSQYTEKSDVYSFGVLVVELLTGKLPVSFARDDGRNLLDHFISLV 545

Query: 545 MTNRLVEILDVTVAKEEMREVVNNVAILAIKCLRSNGRERPTMKQVYLELEGLRRPQDCL 560
            T++L+EILD  VA EE+ +VVN+VA LA KCLRSNG+ERPTMKQVYLELEGLR+ QDC 
Sbjct: 546 KTDQLIEILDFIVATEEIMDVVNHVATLATKCLRSNGKERPTMKQVYLELEGLRKSQDCP 585

BLAST of Lag0035312 vs. NCBI nr
Match: XP_022961349.1 (wall-associated receptor kinase-like 2 [Cucurbita moschata])

HSP 1 Score: 600.9 bits (1548), Expect = 1.1e-167
Identity = 348/643 (54.12%), Postives = 414/643 (64.39%), Query Frame = 0

Query: 1   MEASSPCY-AFSLIIFLFFFPRESICQPTYVGNGS----CSINDT-STPKEYL-CVDSII 60
           MEAS   Y     I+FL  FP +S+ Q  Y+G  +    CSINDT STP  Y  C     
Sbjct: 1   MEASFASYCCLCFILFLSLFPIQSVSQYPYLGTANQTSGCSINDTNSTPNGYYSCKHFPA 60

Query: 61  DSCDSYYLTFKSPPHSYYFSYTNITLGNNILFSTYIYPCKCLGGS-FQNLHSYTIESGDS 120
            SC S YL  KS  H++   + + + GN  L    +  C C GG  + N+  Y   SGDS
Sbjct: 61  GSCSS-YLIAKSKSHNHDLGFYDTSFGNTNLVMKAV-GCSCGGGHLYGNITEYNTTSGDS 120

Query: 121 YEKIVNDYFQGLTSCSALRT--QNHDFAINSSSSTGRQIVVPLRCACPTKAQQANGTESL 180
           YE I N  F G TSCS LR+   NH   IN+SS+ G  + VPLRCACPT+ Q+  G  SL
Sbjct: 121 YETIANHVFYGFTSCSMLRSFNPNHSMDINASSTEGISVEVPLRCACPTQDQKKVGIHSL 180

Query: 181 AMYVLQPNQTIGSVARDFRVDKEFVLKANNLSNSTHAYPFMLILVPLGKYDICRIFGCDS 240
           A+Y++QPN++I S+ARD  VDK+ +L AN LSN+T  YPF  +LVPL  Y+ C++   +S
Sbjct: 181 AVYMVQPNESITSIARDLLVDKDTILDANMLSNTTQLYPFTPLLVPLNTYETCKLDPSNS 240

Query: 241 --------KWRIQ----------------------------------------------- 300
                   +W ++                                               
Sbjct: 241 DACVSPYLEWYLKYAATIGFIIFIAFASGIGMFFCCCGCVGQRLFINRRSRKLHKKKFFR 300

Query: 301 -------NMMLGVDN-----FILEELEQATDNYSQSRFLGQGGFGTVYKGILCDNTLVAI 360
                     L  DN     F+ EELE+AT NYSQ+RFLGQGGFG VYKGIL DNT+VA+
Sbjct: 301 QNGGLVLQQKLLEDNERVKIFLQEELEEATHNYSQTRFLGQGGFGAVYKGILHDNTIVAV 360

Query: 361 KRSKQIEENWIEQFINEVIVLSQINHRNIVRLLGCCLETEFPLLVYEFVSNGTLSHHIHR 420
           KRSK+IEE WIEQFINEVI+LS+INH+NIVRL+GCCLETEFPLLVYEFVSNGTL  H+H 
Sbjct: 361 KRSKRIEEAWIEQFINEVIILSRINHKNIVRLIGCCLETEFPLLVYEFVSNGTLLSHLHH 420

Query: 421 EDPQESPSLSWENRLNIASEVAGAISYMHSAASIPIFHRDIKSLNILLDDNFSAKVSDFG 480
            D  ESPSLSWE RL IASEVAGAISYMHSAAS PIFHRDIKSLNILL+DN++AKVSDFG
Sbjct: 421 TDHGESPSLSWETRLGIASEVAGAISYMHSAASTPIFHRDIKSLNILLEDNYNAKVSDFG 480

Query: 481 TSRFVSCDQSHLTTKVLGTFGYIDPEYFRSSQYTEKSDVYSFGVLLVELLTRKQPVTFPK 540
           TSRFVSCDQSHLTTKV GT GYIDPEYF+SSQYTEKSDVYSFGV++VELLT K P  F +
Sbjct: 481 TSRFVSCDQSHLTTKVHGTLGYIDPEYFKSSQYTEKSDVYSFGVVMVELLTGKHPTIFAR 540

Query: 541 NGGGNLLDYFISLEMTNRLVEILDVTVAK-EEMREVVNNVAILAIKCLRSNGRERPTMKQ 563
           N   NLL+ FI+LE    +V I+D  +AK EE  E VN VA L  KCLR+NG+ERPTMKQ
Sbjct: 541 NEERNLLEDFIALENGKEVVGIVDSAIAKDEETMEGVNLVAKLTAKCLRANGKERPTMKQ 600

BLAST of Lag0035312 vs. NCBI nr
Match: XP_022987096.1 (wall-associated receptor kinase-like 2 [Cucurbita maxima])

HSP 1 Score: 573.9 bits (1478), Expect = 1.5e-159
Identity = 331/638 (51.88%), Postives = 406/638 (63.64%), Query Frame = 0

Query: 1   MEASSPCY-AFSLIIFLFFFPRESICQPTYVGNGS----CSINDT-STPKEYLCVDSIID 60
           MEAS   Y     I+FL  FP +S+ +  Y+G  +    CSINDT STP  Y     +  
Sbjct: 1   MEASFASYCCLCFILFLSLFPIQSVSKYPYLGTANQTSGCSINDTNSTPNGYYSCAYVPY 60

Query: 61  SCDSYYLTFKSPPHSYYFSYTNITLGNNILFSTYIYPCKCLGGS-FQNLHSYTIESGDSY 120
           S  S YL  KS  H+    + + ++GN  L    +  C C G   + N+  Y   SGDSY
Sbjct: 61  SSCSSYLIAKSKSHNQDLGFYDKSIGNTNLLMKAV-GCSCGGDHLYGNIMEYNTTSGDSY 120

Query: 121 EKIVNDYFQGLTSCSALRT--QNHDFAINSSSSTGRQIVVPLRCACPTKAQQANGTESLA 180
           E I N  F G TSCS LR+   NH   IN+SS+ G  + V LRCACPT+ Q+  G +SLA
Sbjct: 121 ETIANHVFYGFTSCSMLRSFNPNHSIDINASSTEGISVEVWLRCACPTQDQKNLGIQSLA 180

Query: 181 MYVLQPNQTIGSVARDFRVDKEFVLKANNLSNSTHAYPFMLILVPLGKYDICRI-----F 240
           +Y++QPN++I S+ARD  V+++ +L+AN LSN+T  YPF  +LVPL   + C+I      
Sbjct: 181 VYMVQPNESISSIARDLLVEQDSILEANMLSNTTQLYPFTPLLVPLNTSETCKIDPSNSD 240

Query: 241 GCDSKW------------------------------------------------------ 300
            C S++                                                      
Sbjct: 241 ACVSRYLKSYLKFVAIAAIISITFAIGIGMFCCGCGCVGQRLFIKRRSRKLCKKKFFRQN 300

Query: 301 -------RIQNMMLGVDNFILEELEQATDNYSQSRFLGQGGFGTVYKGILCDNTLVAIKR 360
                  ++Q     V  F+ EELE+AT +YSQ+RFLGQGGFGTVYKGIL DNT+VA+KR
Sbjct: 301 GGLLLQQKVQEDNERVKIFLQEELEEATHSYSQTRFLGQGGFGTVYKGILHDNTVVAVKR 360

Query: 361 SKQIEENWIEQFINEVIVLSQINHRNIVRLLGCCLETEFPLLVYEFVSNGTLSHHIHRED 420
           SK+IEE WIEQFINEV++LS+INH+NIVRL+GCCLE E+PLLVYEFVSNGTLS H+H  D
Sbjct: 361 SKRIEEAWIEQFINEVVILSRINHKNIVRLIGCCLEAEYPLLVYEFVSNGTLSSHLHHTD 420

Query: 421 PQESPSLSWENRLNIASEVAGAISYMHSAASIPIFHRDIKSLNILLDDNFSAKVSDFGTS 480
             ES SLSWE RL IASEVAGAISYMHSAAS PIFHRDIKSLNILLD+N+SAKVS+FGTS
Sbjct: 421 HGESSSLSWETRLGIASEVAGAISYMHSAASTPIFHRDIKSLNILLDNNYSAKVSEFGTS 480

Query: 481 RFVSCDQSHLTTKVLGTFGYIDPEYFRSSQYTEKSDVYSFGVLLVELLTRKQPVTFPKNG 540
           RFVSCDQSHLTT+V GT GYIDPEYFRSSQYTEKSDVYSFGV++VELLT K P  F +N 
Sbjct: 481 RFVSCDQSHLTTRVHGTLGYIDPEYFRSSQYTEKSDVYSFGVVMVELLTGKHPTIFARNE 540

Query: 541 GGNLLDYFISLEMTNRLVEILDVTVAK-EEMREVVNNVAILAIKCLRSNGRERPTMKQVY 563
             NLL+ FI+LE    +V I+D  +AK EE  E VN VA L  KCLR+NG+E PTMKQVY
Sbjct: 541 ERNLLEDFIALENGKEVVGIVDSAIAKDEETMEGVNLVAKLTAKCLRANGKESPTMKQVY 600

BLAST of Lag0035312 vs. ExPASy Swiss-Prot
Match: Q9S9M5 (Wall-associated receptor kinase-like 1 OS=Arabidopsis thaliana OX=3702 GN=WAKL1 PE=2 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 1.6e-92
Identity = 170/301 (56.48%), Postives = 225/301 (74.75%), Query Frame = 0

Query: 244 FILEELEQATDNYSQSRFLGQGGFGTVYKGILCDNTLVAIKRSKQIEENWIEQFINEVIV 303
           F  +EL +ATDN+S  R LGQGG GTVYKG+L D ++VA+KRSK ++E+ +E+FINE+++
Sbjct: 417 FSSKELRKATDNFSIDRVLGQGGQGTVYKGMLVDGSIVAVKRSKVVDEDKMEEFINEIVL 476

Query: 304 LSQINHRNIVRLLGCCLETEFPLLVYEFVSNGTLSHHIHREDPQESPSLSWENRLNIASE 363
           LSQINHRNIV+LLGCCLETE P+LVYE++ NG L   +H  D  +  +++WE RL IA E
Sbjct: 477 LSQINHRNIVKLLGCCLETEVPILVYEYIPNGDLFKRLH--DESDDYTMTWEVRLRIAIE 536

Query: 364 VAGAISYMHSAASIPIFHRDIKSLNILLDDNFSAKVSDFGTSRFVSCDQSHLTTKVLGTF 423
           +AGA++YMHSAAS PIFHRDIK+ NILLD+ + AKVSDFGTSR V+ DQ+HLTT V GTF
Sbjct: 537 IAGALTYMHSAASFPIFHRDIKTTNILLDEKYRAKVSDFGTSRSVTLDQTHLTTLVAGTF 596

Query: 424 GYIDPEYFRSSQYTEKSDVYSFGVLLVELLTRKQPVTFPKN-GGGNLLDYFISLEMTNRL 483
           GY+DPEYF SSQYT KSDVYSFGV+LVEL+T ++P++  ++  G  L  +F+     NR+
Sbjct: 597 GYMDPEYFLSSQYTHKSDVYSFGVVLVELITGEKPLSRVRSEEGRGLATHFLEAMKENRV 656

Query: 484 VEILDVTVAKEEMREVVNNVAILAIKCLRSNGRERPTMKQVYLELEGLRRPQDCLGIAEE 543
           ++I+D+ +  E   E V  VA LA KCL   G+ RP MK+V  ELE +R   + L +  E
Sbjct: 657 IDIIDIRIKDESKLEQVMAVAKLARKCLNRKGKNRPNMKEVSNELERIRSSPEDLDVRTE 715

BLAST of Lag0035312 vs. ExPASy Swiss-Prot
Match: Q7X8C5 (Wall-associated receptor kinase-like 2 OS=Arabidopsis thaliana OX=3702 GN=WAKL2 PE=2 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.5e-90
Identity = 169/291 (58.08%), Postives = 225/291 (77.32%), Query Frame = 0

Query: 244 FILEELEQATDNYSQSRFLGQGGFGTVYKGILCDNTLVAIKRSKQIEENWIEQFINEVIV 303
           F   ELE+ATDN++++R LGQGG GTVYKG+L D  +VA+KRSK ++E+ +E+FINEV+V
Sbjct: 404 FSSHELEKATDNFNKNRVLGQGGQGTVYKGMLVDGRIVAVKRSKAVDEDRVEEFINEVVV 463

Query: 304 LSQINHRNIVRLLGCCLETEFPLLVYEFVSNGTLSHHIHREDPQESPSLSWENRLNIASE 363
           L+QINHRNIV+LLGCCLETE P+LVYEFV NG L   +H  D  +  +++WE RL+IA E
Sbjct: 464 LAQINHRNIVKLLGCCLETEVPVLVYEFVPNGDLCKRLH--DESDDYTMTWEVRLHIAIE 523

Query: 364 VAGAISYMHSAASIPIFHRDIKSLNILLDDNFSAKVSDFGTSRFVSCDQSHLTTKVLGTF 423
           +AGA+SY+HSAAS PI+HRDIK+ NILLD+   AKVSDFGTSR V+ DQ+HLTT+V GTF
Sbjct: 524 IAGALSYLHSAASFPIYHRDIKTTNILLDERNRAKVSDFGTSRSVTIDQTHLTTQVAGTF 583

Query: 424 GYIDPEYFRSSQYTEKSDVYSFGVLLVELLTRKQP---VTFPKNGGGNLLDYFISLEMTN 483
           GY+DPEYF+SS++TEKSDVYSFGV+LVELLT ++P   V   +N G  L  +F+     N
Sbjct: 584 GYVDPEYFQSSKFTEKSDVYSFGVVLVELLTGEKPSSRVRSEENRG--LAAHFVEAVKEN 643

Query: 484 RLVEILDVTVAKEEMREVVNNVAILAIKCLRSNGRERPTMKQVYLELEGLR 532
           R+++I+D  +  E   + V +VA LA +CL   G++RP M++V +ELE +R
Sbjct: 644 RVLDIVDDRIKDECNMDQVMSVANLARRCLNRKGKKRPNMREVSIELEMIR 690

BLAST of Lag0035312 vs. ExPASy Swiss-Prot
Match: Q9S9M3 (Wall-associated receptor kinase-like 3 OS=Arabidopsis thaliana OX=3702 GN=WAKL3 PE=2 SV=2)

HSP 1 Score: 334.0 bits (855), Expect = 3.4e-90
Identity = 172/319 (53.92%), Postives = 232/319 (72.73%), Query Frame = 0

Query: 244 FILEELEQATDNYSQSRFLGQGGFGTVYKGILCDNTLVAIKRSKQIEENWIEQFINEVIV 303
           F   ELE+ATDN+S  R LGQGG GTVYK +L D ++VA+KRSK ++E+ +E+FINE+++
Sbjct: 416 FSSRELEKATDNFSIDRVLGQGGQGTVYKRMLVDGSIVAVKRSKVVDEDKMEEFINEIVL 475

Query: 304 LSQINHRNIVRLLGCCLETEFPLLVYEFVSNGTLSHHIHREDPQESPSLSWENRLNIASE 363
           LSQINHRNIV+LLGCCLETE P+LVYE++ NG L   +H  D  +   ++WE RL IA E
Sbjct: 476 LSQINHRNIVKLLGCCLETEVPILVYEYIPNGDLFKRLH--DEYDDYMMTWEVRLRIAVE 535

Query: 364 VAGAISYMHSAASIPIFHRDIKSLNILLDDNFSAKVSDFGTSRFVSCDQSHLTTKVLGTF 423
           +AGA+SYMHSAAS PIFHRDIK+ NILLD+ + AK+SDFGTSR V+ DQ+HLTT V GTF
Sbjct: 536 IAGALSYMHSAASFPIFHRDIKTTNILLDEKYRAKISDFGTSRSVATDQTHLTTLVAGTF 595

Query: 424 GYIDPEYFRSSQYTEKSDVYSFGVLLVELLTRKQPVTFPKNGGG-NLLDYFISLEMTNRL 483
           GY+DPEYF SSQYT KSDVYSFGV+LVEL+T ++P++  ++  G  L  YF+     NR 
Sbjct: 596 GYMDPEYFLSSQYTHKSDVYSFGVVLVELITGEKPMSRVRSEEGIGLATYFLEAMKENRA 655

Query: 484 VEILDVTVAKEEMREVVNNVAILAIKCLRSNGRERPTMKQVYLELEGLRRPQDCLGIAEE 543
           V+I+D+ + K+E ++V+  VA LA +CL   G +RP M++V ++LE +R     L +  E
Sbjct: 656 VDIIDIRI-KDESKQVM-AVAKLARRCLNRKGNKRPNMREVSIKLERIRSSPKDLDVHTE 715

Query: 544 SHSRTFSLHSFESSDDELL 562
           +          E  +D+L+
Sbjct: 716 NEE--------EEEEDQLM 722

BLAST of Lag0035312 vs. ExPASy Swiss-Prot
Match: Q9S9M1 (Wall-associated receptor kinase-like 5 OS=Arabidopsis thaliana OX=3702 GN=WAKL5 PE=2 SV=2)

HSP 1 Score: 332.0 bits (850), Expect = 1.3e-89
Identity = 162/294 (55.10%), Postives = 222/294 (75.51%), Query Frame = 0

Query: 244 FILEELEQATDNYSQSRFLGQGGFGTVYKGILCDNTLVAIKRSKQIEENWIEQFINEVIV 303
           F  EEL++ATDN+S  R LG+G  GTVYKG++ D  ++A+KRSK ++E+ +E+FINE+I+
Sbjct: 420 FSSEELKKATDNFSVKRVLGKGSQGTVYKGMMVDGKIIAVKRSKVVDEDKLEKFINEIIL 479

Query: 304 LSQINHRNIVRLLGCCLETEFPLLVYEFVSNGTLSHHIHREDPQESPSLSWENRLNIASE 363
           LSQINHRNIV+L+GCCLETE P+LVYE++ NG +   +H  D  +  +++WE RL IA E
Sbjct: 480 LSQINHRNIVKLIGCCLETEVPILVYEYIPNGDMFKRLH--DESDDYAMTWEVRLRIAIE 539

Query: 364 VAGAISYMHSAASIPIFHRDIKSLNILLDDNFSAKVSDFGTSRFVSCDQSHLTTKVLGTF 423
           +AGA++YMHSAAS PI+HRDIK+ NILLD+ + AKVSDFGTSR V+ DQ+HLTT V GTF
Sbjct: 540 IAGALTYMHSAASFPIYHRDIKTTNILLDEKYGAKVSDFGTSRSVTIDQTHLTTMVAGTF 599

Query: 424 GYIDPEYFRSSQYTEKSDVYSFGVLLVELLTRKQPVT-FPKNGGGNLLDYFISLEMTNRL 483
           GY+DPEYF SSQYT+KSDVYSFGV+LVEL+T ++P++      G  L  +F+     NR+
Sbjct: 600 GYMDPEYFLSSQYTDKSDVYSFGVVLVELITGEKPLSRIRSEEGRGLATHFLEAMKENRV 659

Query: 484 VEILDVTVAKEEMREVVNNVAILAIKCLRSNGRERPTMKQVYLELEGLR-RPQD 536
           ++I+D+ + +E   + +  VA LA KCL   G +RP M++  LELE +R  P+D
Sbjct: 660 IDIIDIRIKEESKLDQLMAVAKLARKCLSRKGIKRPNMREASLELERIRSSPED 711

BLAST of Lag0035312 vs. ExPASy Swiss-Prot
Match: Q9C9L5 (Wall-associated receptor kinase-like 9 OS=Arabidopsis thaliana OX=3702 GN=WAKL9 PE=2 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 4.9e-89
Identity = 164/314 (52.23%), Postives = 227/314 (72.29%), Query Frame = 0

Query: 244 FILEELEQATDNYSQSRFLGQGGFGTVYKGILCDNTLVAIKRSKQIEENWIEQFINEVIV 303
           F   ELE+AT+N+S +R LGQGG GTVYKG+L D  +VA+K+SK ++E+ +E+FINEV++
Sbjct: 435 FSSRELEKATENFSSNRILGQGGQGTVYKGMLVDGRIVAVKKSKVVDEDKLEEFINEVVI 494

Query: 304 LSQINHRNIVRLLGCCLETEFPLLVYEFVSNGTLSHHIHREDPQESPSLSWENRLNIASE 363
           LSQINHRNIV+LLGCCLET+ P+LVYEF+ NG L  H+H ++  E+   +W  RL IA +
Sbjct: 495 LSQINHRNIVKLLGCCLETKVPVLVYEFIPNGNLFEHLH-DEFDENIMATWNIRLRIAID 554

Query: 364 VAGAISYMHSAASIPIFHRDIKSLNILLDDNFSAKVSDFGTSRFVSCDQSHLTTKVLGTF 423
           +AGA+SY+HS+AS PI+HRD+KS NI+LD+ + AKVSDFGTSR V+ D +HLTT V GT 
Sbjct: 555 IAGALSYLHSSASSPIYHRDVKSTNIMLDEKYRAKVSDFGTSRTVTVDHTHLTTVVSGTV 614

Query: 424 GYIDPEYFRSSQYTEKSDVYSFGVLLVELLTRKQPVTFPKN-GGGNLLDYFISLEMTNRL 483
           GY+DPEYF+SSQ+T+KSDVYSFGV+LVEL+T ++ ++F ++     L  YFI     N+L
Sbjct: 615 GYMDPEYFQSSQFTDKSDVYSFGVVLVELITGEKSISFLRSQENRTLATYFILAMKENKL 674

Query: 484 VEILDVTVAKEEMREVVNNVAILAIKCLRSNGRERPTMKQVYLELEGLRRP------QDC 543
            +I+D  +    M   V   A +A KCL   GR+RP+M++V +EL+ +R P      Q+C
Sbjct: 675 FDIIDARIRDGCMLSQVTATAKVARKCLNLKGRKRPSMREVSMELDSIRMPCGDMQLQEC 734

Query: 544 LGIAEESHSRTFSL 551
           +   EE   +   L
Sbjct: 735 VSENEEGEEQNKGL 747

BLAST of Lag0035312 vs. ExPASy TrEMBL
Match: A0A6J1DGJ3 (wall-associated receptor kinase-like 1 OS=Momordica charantia OX=3673 GN=LOC111019878 PE=4 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 3.0e-198
Identity = 380/618 (61.49%), Postives = 445/618 (72.01%), Query Frame = 0

Query: 12  LIIFLFFFPRESICQPTYVGN-GSCSINDTSTPKEYLCVDSIIDSCDSYYLTFKSPPHSY 71
           +I+F   F  +S CQ +Y+GN  SCSIN+ S PKEYLC DSI  SCDSY+  F+   H  
Sbjct: 14  IILFFSLFLYQSFCQQSYLGNETSCSINNNSGPKEYLCTDSIY-SCDSYF-AFRYELHFG 73

Query: 72  YFSYTNITLGNNILFSTYIYPCKCLGGSFQNLHSYTIESGDSYEKIVNDYFQGLTSCSAL 131
            F Y NITLG+N L    +  C CLG S+Q    YTI+SGDSYEKI +D FQGLTSCSAL
Sbjct: 74  NFYYFNITLGDNNLSIAKVL-CSCLGSSYQYNMLYTIKSGDSYEKIAHDVFQGLTSCSAL 133

Query: 132 RTQNHDFAINSSSSTGRQIVVPLRCACPTKAQQANGTESLAMYVLQPNQTIGSVARDFRV 191
           + +NHD+ + ++++ GR++ VP+RCACPTKAQ A+   SL +Y LQ N+TIGSVAR F V
Sbjct: 134 KNKNHDYPLVNTAAIGRRLEVPIRCACPTKAQIASRVHSLIVYTLQLNETIGSVARAFGV 193

Query: 192 DKEFVLKANNLSNSTHAYPFMLILVPLGKYDICRIF------------------------ 251
           +++ VL+AN LSN +H YPF  ILVPLGK D CRI+                        
Sbjct: 194 EEKSVLEANMLSNWSHIYPFTPILVPLGKDDTCRIYPYSLDCRDMSSSFSDIFSYIFLNL 253

Query: 252 -----------------GC----------------------DSKWRIQNMML----GVDN 311
                            GC                      +    +Q  +L     V  
Sbjct: 254 VLIPIAGLIGLACCFCCGCKGNQLLLKKRRRKLYKKKFFKQNGGHLLQQKLLEDSERVKI 313

Query: 312 FILEELEQATDNYSQSRFLGQGGFGTVYKGILCDNTLVAIKRSKQIEENWIEQFINEVIV 371
           +  EELEQATDNY+ SRFLGQGG+GTVYKGIL DNT+VAIKRSKQI+ +WIEQFINEVI+
Sbjct: 314 YTQEELEQATDNYNDSRFLGQGGYGTVYKGILHDNTVVAIKRSKQIDTSWIEQFINEVII 373

Query: 372 LSQINHRNIVRLLGCCLETEFPLLVYEFVSNGTLSHHIHREDPQESPSLSWENRLNIASE 431
           LSQINHRNIV+LLGCCLETEFPLLVYEFV NGTLSHHIH+E+ +ESP LSWE RL IASE
Sbjct: 374 LSQINHRNIVKLLGCCLETEFPLLVYEFVYNGTLSHHIHKENYEESPPLSWETRLRIASE 433

Query: 432 VAGAISYMHSAASIPIFHRDIKSLNILLDDNFSAKVSDFGTSRFVSCDQSHLTTKVLGTF 491
           V+GA+SYMHSAASIPI+HRDIKSLNILLD+N+SAKVSDFGTSRFVSCDQSHLTTKVLGTF
Sbjct: 434 VSGAMSYMHSAASIPIYHRDIKSLNILLDENYSAKVSDFGTSRFVSCDQSHLTTKVLGTF 493

Query: 492 GYIDPEYFRSSQYTEKSDVYSFGVLLVELLTRKQPVTFPKNGGGNLLDYFISLEMTNRLV 551
           GYIDPEYFRSSQYTEKSDVYSFGVL+VELLT K PVTF ++ G NLLDYFISLE  N+LV
Sbjct: 494 GYIDPEYFRSSQYTEKSDVYSFGVLMVELLTGKLPVTFARDDGRNLLDYFISLEKANQLV 553

Query: 552 EILDVTVAKEEMREVVNNVAILAIKCLRSNGRERPTMKQVYLELEGLRRPQDCLGIAEES 561
           EILD+ VA EE  +VVN+VA LA KCLRSNG+ERPTMKQVYLELEGLR+ +DC  I+E+ 
Sbjct: 554 EILDIIVAAEESMDVVNSVATLATKCLRSNGKERPTMKQVYLELEGLRKSRDCPDISEDV 613

BLAST of Lag0035312 vs. ExPASy TrEMBL
Match: A0A6J1DGL8 (wall-associated receptor kinase-like 1 OS=Momordica charantia OX=3673 GN=LOC111019885 PE=4 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 3.2e-192
Identity = 372/611 (60.88%), Postives = 427/611 (69.89%), Query Frame = 0

Query: 11  SLIIFLFFFPRESICQPTYVGN-GSCSINDTSTPKEYLCVDSIIDSCDSYYLTFKSPPHS 70
           SL+  LFFFP +S+CQ  Y+GN  +CS+NDTST K Y+C DSI  SC SY +       S
Sbjct: 3   SLLFSLFFFPLQSLCQQPYIGNQTNCSMNDTSTSKGYICTDSIF-SCQSYLIFRSDSLLS 62

Query: 71  YYFSYTNITLGNNILFSTYIYPCKCLGGSFQNLHSYTIESGDSYEKIVNDYFQGLTSCSA 130
           Y     N TLG N LF T    C C G  +  +  YTI+ GDSYEKIVND FQG TSCS 
Sbjct: 63  YDLYLLNFTLGKNTLFRT-TADCGCWGLYYHYIAYYTIKKGDSYEKIVNDIFQGFTSCSV 122

Query: 131 LRTQNHDFAINSSSSTGRQIVVPLRCACPTKAQQANGTESLAMYVLQPNQTIGSVARDFR 190
           LR+ + D+AI+++SS GR++ VPL CACPTKAQ+A G E LA+Y+LQPN++IGSVARDF 
Sbjct: 123 LRSVSLDYAIDNTSSVGRRLQVPLGCACPTKAQKAKGVEFLAVYMLQPNESIGSVARDFG 182

Query: 191 VDKEFVLKANNLSNSTHAYPFMLILVPLGKYDICRI------------------------ 250
           V++E +L+AN LS S+  +PF  IL+PL K   CRI                        
Sbjct: 183 VEEEGILEANMLSMSSQTFPFTPILIPLNKNVTCRIDPSSYYDCNAIPPPTSNDNDYILY 242

Query: 251 ---------------FGCDSKWR------------------------IQNMML----GVD 310
                          F C  K                          +Q  +L     V 
Sbjct: 243 IVLIAIGGVIVLVSCFFCGCKGHQLLLRKRRRKLHTKKFFRQNGGLLLQQKLLENSERVK 302

Query: 311 NFILEELEQATDNYSQSRFLGQGGFGTVYKGILCDNTLVAIKRSKQIEENWIEQFINEVI 370
            F  EELEQATDNY++SRFLGQGG+GTVYKG+L DNT+VAIKRS QIE NWI+QFINEVI
Sbjct: 303 IFTHEELEQATDNYNESRFLGQGGYGTVYKGMLNDNTIVAIKRSNQIEANWIDQFINEVI 362

Query: 371 VLSQINHRNIVRLLGCCLETEFPLLVYEFVSNGTLSHHIHREDPQESPSLSWENRLNIAS 430
           +LSQINHRNIVRLLGCCLETE PLLVYEFVSNGTLSHHI+++D  ESPSLSWE RL IAS
Sbjct: 363 ILSQINHRNIVRLLGCCLETEHPLLVYEFVSNGTLSHHINQKDHGESPSLSWETRLRIAS 422

Query: 431 EVAGAISYMHSAASIPIFHRDIKSLNILLDDNFSAKVSDFGTSRFVSCDQSHLTTKVLGT 490
           EVAGAISYMHS AS  IFH+DIKSLNILLDDN+  KVSDFGTSRFVS DQSHLTTKV GT
Sbjct: 423 EVAGAISYMHSTASTQIFHKDIKSLNILLDDNYHTKVSDFGTSRFVSSDQSHLTTKVQGT 482

Query: 491 FGYIDPEYFRSSQYTEKSDVYSFGVLLVELLTRKQPVTFPKNGGGNLLDYFISLEMTNRL 550
           FGYIDPEYFRSS YTEKSDVY+FGVL+VELLT K PVTF K+ G NLL+YFISLE  N+L
Sbjct: 483 FGYIDPEYFRSSHYTEKSDVYNFGVLMVELLTGKSPVTFAKHEGRNLLEYFISLEKANQL 542

Query: 551 VEILDVTVAKEEMREVVNNVAILAIKCLRSNGRERPTMKQVYLELEGLRRPQDCLGIAEE 553
           VEILD+ VAKEE  EVVN VA L  KCLRSNG+ERPTMK VYLELE  R+PQ+CL I EE
Sbjct: 543 VEILDIAVAKEEKMEVVNAVAKLTTKCLRSNGKERPTMKHVYLELERSRKPQNCLAIIEE 602

BLAST of Lag0035312 vs. ExPASy TrEMBL
Match: A0A6J1DF11 (putative wall-associated receptor kinase-like 16 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019884 PE=4 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 2.2e-185
Identity = 366/623 (58.75%), Postives = 425/623 (68.22%), Query Frame = 0

Query: 5   SPCYAFSLII--FLFFFPRESICQPTYVG-NGSCSINDTSTPKEYLCVDSIIDSCDSYYL 64
           S C+A  LII   +  FP +S  Q +Y+G   SCSIN+ STP+E                
Sbjct: 6   SSCHALPLIILFLISLFPYQSFSQQSYLGKETSCSINNNSTPQE---------------- 65

Query: 65  TFKSPPHSYYFSYTNITLGNNILFSTYIYPCKCLGGSFQNLHSYTIESGDSYEKIVNDYF 124
                                     Y++ C C G S+Q +  YTI+SGDSYEKI  D F
Sbjct: 66  --------------------------YVWSCSCFGHSYQYIIDYTIKSGDSYEKIAYDIF 125

Query: 125 QGLTSCSALRTQNHDFAINSSSSTGRQIVVPLRCACPTKAQQANGTESLAMYVLQPNQTI 184
           QGLTSCSAL+ +N D+ +NS+++ GR++ VPLRCACPTKAQ A+  ++L +Y+LQ N+TI
Sbjct: 126 QGLTSCSALKNKNDDYPLNSTAAIGRRLEVPLRCACPTKAQNASRIDNLVVYMLQLNETI 185

Query: 185 GSVARDFRVDKEFVLKANNLSNSTHAYPFMLILVPLGKYDICRIF--------------- 244
           GSVAR F V+++ VL+AN LSN +H YPF  ILVPLGK D CRI+               
Sbjct: 186 GSVARAFGVEEKSVLEANMLSNWSHIYPFTPILVPLGKDDTCRIYPYGDCSSNSNSYLLP 245

Query: 245 -----------------------GC----------------------DSKWRIQNMML-- 304
                                  GC                      +  + +Q  +L  
Sbjct: 246 FIFLYLVTIPIGGLITLACCFFCGCKGNQLLLKKRRRKLYKKKFFKQNGGFLLQQKLLED 305

Query: 305 --GVDNFILEELEQATDNYSQSRFLGQGGFGTVYKGILCDNTLVAIKRSKQIEENWIEQF 364
              V  F  EELEQATDNYS SR LGQGG+GTVYKG+L DNT VAIKRSKQIE +WIEQF
Sbjct: 306 SERVKVFTEEELEQATDNYSDSRLLGQGGYGTVYKGMLRDNTTVAIKRSKQIETSWIEQF 365

Query: 365 INEVIVLSQINHRNIVRLLGCCLETEFPLLVYEFVSNGTLSHHIHREDPQESPSLSWENR 424
           INEVIVLSQINHRNIVRLLGCCLETEFPLLVYEFV NGTLSHHIH E+ ++SP LSWE R
Sbjct: 366 INEVIVLSQINHRNIVRLLGCCLETEFPLLVYEFVPNGTLSHHIHEENLEDSP-LSWEAR 425

Query: 425 LNIASEVAGAISYMHSAASIPIFHRDIKSLNILLDDNFSAKVSDFGTSRFVSCDQSHLTT 484
           L IASEVAGAISYMHSAASIPI+HRDIKSLNILLDDNFSAKVSDFGTSRFVSCDQSHLTT
Sbjct: 426 LRIASEVAGAISYMHSAASIPIYHRDIKSLNILLDDNFSAKVSDFGTSRFVSCDQSHLTT 485

Query: 485 KVLGTFGYIDPEYFRSSQYTEKSDVYSFGVLLVELLTRKQPVTFPKNGGGNLLDYFISLE 544
           KVLGTFGYIDPEYFRSSQYTEKSDVYSFGVL+VELLT K PV+F ++ G NLLD+FISL 
Sbjct: 486 KVLGTFGYIDPEYFRSSQYTEKSDVYSFGVLVVELLTGKLPVSFARDDGRNLLDHFISLV 545

Query: 545 MTNRLVEILDVTVAKEEMREVVNNVAILAIKCLRSNGRERPTMKQVYLELEGLRRPQDCL 560
            T++L+EILD  VA EE+ +VVN+VA LA KCLRSNG+ERPTMKQVYLELEGLR+ QDC 
Sbjct: 546 KTDQLIEILDFIVATEEIMDVVNHVATLATKCLRSNGKERPTMKQVYLELEGLRKSQDCP 585

BLAST of Lag0035312 vs. ExPASy TrEMBL
Match: A0A6J1HDS3 (wall-associated receptor kinase-like 2 OS=Cucurbita moschata OX=3662 GN=LOC111461865 PE=4 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 5.5e-168
Identity = 348/643 (54.12%), Postives = 414/643 (64.39%), Query Frame = 0

Query: 1   MEASSPCY-AFSLIIFLFFFPRESICQPTYVGNGS----CSINDT-STPKEYL-CVDSII 60
           MEAS   Y     I+FL  FP +S+ Q  Y+G  +    CSINDT STP  Y  C     
Sbjct: 1   MEASFASYCCLCFILFLSLFPIQSVSQYPYLGTANQTSGCSINDTNSTPNGYYSCKHFPA 60

Query: 61  DSCDSYYLTFKSPPHSYYFSYTNITLGNNILFSTYIYPCKCLGGS-FQNLHSYTIESGDS 120
            SC S YL  KS  H++   + + + GN  L    +  C C GG  + N+  Y   SGDS
Sbjct: 61  GSCSS-YLIAKSKSHNHDLGFYDTSFGNTNLVMKAV-GCSCGGGHLYGNITEYNTTSGDS 120

Query: 121 YEKIVNDYFQGLTSCSALRT--QNHDFAINSSSSTGRQIVVPLRCACPTKAQQANGTESL 180
           YE I N  F G TSCS LR+   NH   IN+SS+ G  + VPLRCACPT+ Q+  G  SL
Sbjct: 121 YETIANHVFYGFTSCSMLRSFNPNHSMDINASSTEGISVEVPLRCACPTQDQKKVGIHSL 180

Query: 181 AMYVLQPNQTIGSVARDFRVDKEFVLKANNLSNSTHAYPFMLILVPLGKYDICRIFGCDS 240
           A+Y++QPN++I S+ARD  VDK+ +L AN LSN+T  YPF  +LVPL  Y+ C++   +S
Sbjct: 181 AVYMVQPNESITSIARDLLVDKDTILDANMLSNTTQLYPFTPLLVPLNTYETCKLDPSNS 240

Query: 241 --------KWRIQ----------------------------------------------- 300
                   +W ++                                               
Sbjct: 241 DACVSPYLEWYLKYAATIGFIIFIAFASGIGMFFCCCGCVGQRLFINRRSRKLHKKKFFR 300

Query: 301 -------NMMLGVDN-----FILEELEQATDNYSQSRFLGQGGFGTVYKGILCDNTLVAI 360
                     L  DN     F+ EELE+AT NYSQ+RFLGQGGFG VYKGIL DNT+VA+
Sbjct: 301 QNGGLVLQQKLLEDNERVKIFLQEELEEATHNYSQTRFLGQGGFGAVYKGILHDNTIVAV 360

Query: 361 KRSKQIEENWIEQFINEVIVLSQINHRNIVRLLGCCLETEFPLLVYEFVSNGTLSHHIHR 420
           KRSK+IEE WIEQFINEVI+LS+INH+NIVRL+GCCLETEFPLLVYEFVSNGTL  H+H 
Sbjct: 361 KRSKRIEEAWIEQFINEVIILSRINHKNIVRLIGCCLETEFPLLVYEFVSNGTLLSHLHH 420

Query: 421 EDPQESPSLSWENRLNIASEVAGAISYMHSAASIPIFHRDIKSLNILLDDNFSAKVSDFG 480
            D  ESPSLSWE RL IASEVAGAISYMHSAAS PIFHRDIKSLNILL+DN++AKVSDFG
Sbjct: 421 TDHGESPSLSWETRLGIASEVAGAISYMHSAASTPIFHRDIKSLNILLEDNYNAKVSDFG 480

Query: 481 TSRFVSCDQSHLTTKVLGTFGYIDPEYFRSSQYTEKSDVYSFGVLLVELLTRKQPVTFPK 540
           TSRFVSCDQSHLTTKV GT GYIDPEYF+SSQYTEKSDVYSFGV++VELLT K P  F +
Sbjct: 481 TSRFVSCDQSHLTTKVHGTLGYIDPEYFKSSQYTEKSDVYSFGVVMVELLTGKHPTIFAR 540

Query: 541 NGGGNLLDYFISLEMTNRLVEILDVTVAK-EEMREVVNNVAILAIKCLRSNGRERPTMKQ 563
           N   NLL+ FI+LE    +V I+D  +AK EE  E VN VA L  KCLR+NG+ERPTMKQ
Sbjct: 541 NEERNLLEDFIALENGKEVVGIVDSAIAKDEETMEGVNLVAKLTAKCLRANGKERPTMKQ 600

BLAST of Lag0035312 vs. ExPASy TrEMBL
Match: A0A6J1JD61 (wall-associated receptor kinase-like 2 OS=Cucurbita maxima OX=3661 GN=LOC111484752 PE=4 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 7.2e-160
Identity = 331/638 (51.88%), Postives = 406/638 (63.64%), Query Frame = 0

Query: 1   MEASSPCY-AFSLIIFLFFFPRESICQPTYVGNGS----CSINDT-STPKEYLCVDSIID 60
           MEAS   Y     I+FL  FP +S+ +  Y+G  +    CSINDT STP  Y     +  
Sbjct: 1   MEASFASYCCLCFILFLSLFPIQSVSKYPYLGTANQTSGCSINDTNSTPNGYYSCAYVPY 60

Query: 61  SCDSYYLTFKSPPHSYYFSYTNITLGNNILFSTYIYPCKCLGGS-FQNLHSYTIESGDSY 120
           S  S YL  KS  H+    + + ++GN  L    +  C C G   + N+  Y   SGDSY
Sbjct: 61  SSCSSYLIAKSKSHNQDLGFYDKSIGNTNLLMKAV-GCSCGGDHLYGNIMEYNTTSGDSY 120

Query: 121 EKIVNDYFQGLTSCSALRT--QNHDFAINSSSSTGRQIVVPLRCACPTKAQQANGTESLA 180
           E I N  F G TSCS LR+   NH   IN+SS+ G  + V LRCACPT+ Q+  G +SLA
Sbjct: 121 ETIANHVFYGFTSCSMLRSFNPNHSIDINASSTEGISVEVWLRCACPTQDQKNLGIQSLA 180

Query: 181 MYVLQPNQTIGSVARDFRVDKEFVLKANNLSNSTHAYPFMLILVPLGKYDICRI-----F 240
           +Y++QPN++I S+ARD  V+++ +L+AN LSN+T  YPF  +LVPL   + C+I      
Sbjct: 181 VYMVQPNESISSIARDLLVEQDSILEANMLSNTTQLYPFTPLLVPLNTSETCKIDPSNSD 240

Query: 241 GCDSKW------------------------------------------------------ 300
            C S++                                                      
Sbjct: 241 ACVSRYLKSYLKFVAIAAIISITFAIGIGMFCCGCGCVGQRLFIKRRSRKLCKKKFFRQN 300

Query: 301 -------RIQNMMLGVDNFILEELEQATDNYSQSRFLGQGGFGTVYKGILCDNTLVAIKR 360
                  ++Q     V  F+ EELE+AT +YSQ+RFLGQGGFGTVYKGIL DNT+VA+KR
Sbjct: 301 GGLLLQQKVQEDNERVKIFLQEELEEATHSYSQTRFLGQGGFGTVYKGILHDNTVVAVKR 360

Query: 361 SKQIEENWIEQFINEVIVLSQINHRNIVRLLGCCLETEFPLLVYEFVSNGTLSHHIHRED 420
           SK+IEE WIEQFINEV++LS+INH+NIVRL+GCCLE E+PLLVYEFVSNGTLS H+H  D
Sbjct: 361 SKRIEEAWIEQFINEVVILSRINHKNIVRLIGCCLEAEYPLLVYEFVSNGTLSSHLHHTD 420

Query: 421 PQESPSLSWENRLNIASEVAGAISYMHSAASIPIFHRDIKSLNILLDDNFSAKVSDFGTS 480
             ES SLSWE RL IASEVAGAISYMHSAAS PIFHRDIKSLNILLD+N+SAKVS+FGTS
Sbjct: 421 HGESSSLSWETRLGIASEVAGAISYMHSAASTPIFHRDIKSLNILLDNNYSAKVSEFGTS 480

Query: 481 RFVSCDQSHLTTKVLGTFGYIDPEYFRSSQYTEKSDVYSFGVLLVELLTRKQPVTFPKNG 540
           RFVSCDQSHLTT+V GT GYIDPEYFRSSQYTEKSDVYSFGV++VELLT K P  F +N 
Sbjct: 481 RFVSCDQSHLTTRVHGTLGYIDPEYFRSSQYTEKSDVYSFGVVMVELLTGKHPTIFARNE 540

Query: 541 GGNLLDYFISLEMTNRLVEILDVTVAK-EEMREVVNNVAILAIKCLRSNGRERPTMKQVY 563
             NLL+ FI+LE    +V I+D  +AK EE  E VN VA L  KCLR+NG+E PTMKQVY
Sbjct: 541 ERNLLEDFIALENGKEVVGIVDSAIAKDEETMEGVNLVAKLTAKCLRANGKESPTMKQVY 600

BLAST of Lag0035312 vs. TAIR 10
Match: AT1G16120.1 (wall associated kinase-like 1 )

HSP 1 Score: 341.7 bits (875), Expect = 1.2e-93
Identity = 170/301 (56.48%), Postives = 225/301 (74.75%), Query Frame = 0

Query: 244 FILEELEQATDNYSQSRFLGQGGFGTVYKGILCDNTLVAIKRSKQIEENWIEQFINEVIV 303
           F  +EL +ATDN+S  R LGQGG GTVYKG+L D ++VA+KRSK ++E+ +E+FINE+++
Sbjct: 417 FSSKELRKATDNFSIDRVLGQGGQGTVYKGMLVDGSIVAVKRSKVVDEDKMEEFINEIVL 476

Query: 304 LSQINHRNIVRLLGCCLETEFPLLVYEFVSNGTLSHHIHREDPQESPSLSWENRLNIASE 363
           LSQINHRNIV+LLGCCLETE P+LVYE++ NG L   +H  D  +  +++WE RL IA E
Sbjct: 477 LSQINHRNIVKLLGCCLETEVPILVYEYIPNGDLFKRLH--DESDDYTMTWEVRLRIAIE 536

Query: 364 VAGAISYMHSAASIPIFHRDIKSLNILLDDNFSAKVSDFGTSRFVSCDQSHLTTKVLGTF 423
           +AGA++YMHSAAS PIFHRDIK+ NILLD+ + AKVSDFGTSR V+ DQ+HLTT V GTF
Sbjct: 537 IAGALTYMHSAASFPIFHRDIKTTNILLDEKYRAKVSDFGTSRSVTLDQTHLTTLVAGTF 596

Query: 424 GYIDPEYFRSSQYTEKSDVYSFGVLLVELLTRKQPVTFPKN-GGGNLLDYFISLEMTNRL 483
           GY+DPEYF SSQYT KSDVYSFGV+LVEL+T ++P++  ++  G  L  +F+     NR+
Sbjct: 597 GYMDPEYFLSSQYTHKSDVYSFGVVLVELITGEKPLSRVRSEEGRGLATHFLEAMKENRV 656

Query: 484 VEILDVTVAKEEMREVVNNVAILAIKCLRSNGRERPTMKQVYLELEGLRRPQDCLGIAEE 543
           ++I+D+ +  E   E V  VA LA KCL   G+ RP MK+V  ELE +R   + L +  E
Sbjct: 657 IDIIDIRIKDESKLEQVMAVAKLARKCLNRKGKNRPNMKEVSNELERIRSSPEDLDVRTE 715

BLAST of Lag0035312 vs. TAIR 10
Match: AT1G16130.1 (wall associated kinase-like 2 )

HSP 1 Score: 335.1 bits (858), Expect = 1.1e-91
Identity = 169/291 (58.08%), Postives = 225/291 (77.32%), Query Frame = 0

Query: 244 FILEELEQATDNYSQSRFLGQGGFGTVYKGILCDNTLVAIKRSKQIEENWIEQFINEVIV 303
           F   ELE+ATDN++++R LGQGG GTVYKG+L D  +VA+KRSK ++E+ +E+FINEV+V
Sbjct: 404 FSSHELEKATDNFNKNRVLGQGGQGTVYKGMLVDGRIVAVKRSKAVDEDRVEEFINEVVV 463

Query: 304 LSQINHRNIVRLLGCCLETEFPLLVYEFVSNGTLSHHIHREDPQESPSLSWENRLNIASE 363
           L+QINHRNIV+LLGCCLETE P+LVYEFV NG L   +H  D  +  +++WE RL+IA E
Sbjct: 464 LAQINHRNIVKLLGCCLETEVPVLVYEFVPNGDLCKRLH--DESDDYTMTWEVRLHIAIE 523

Query: 364 VAGAISYMHSAASIPIFHRDIKSLNILLDDNFSAKVSDFGTSRFVSCDQSHLTTKVLGTF 423
           +AGA+SY+HSAAS PI+HRDIK+ NILLD+   AKVSDFGTSR V+ DQ+HLTT+V GTF
Sbjct: 524 IAGALSYLHSAASFPIYHRDIKTTNILLDERNRAKVSDFGTSRSVTIDQTHLTTQVAGTF 583

Query: 424 GYIDPEYFRSSQYTEKSDVYSFGVLLVELLTRKQP---VTFPKNGGGNLLDYFISLEMTN 483
           GY+DPEYF+SS++TEKSDVYSFGV+LVELLT ++P   V   +N G  L  +F+     N
Sbjct: 584 GYVDPEYFQSSKFTEKSDVYSFGVVLVELLTGEKPSSRVRSEENRG--LAAHFVEAVKEN 643

Query: 484 RLVEILDVTVAKEEMREVVNNVAILAIKCLRSNGRERPTMKQVYLELEGLR 532
           R+++I+D  +  E   + V +VA LA +CL   G++RP M++V +ELE +R
Sbjct: 644 RVLDIVDDRIKDECNMDQVMSVANLARRCLNRKGKKRPNMREVSIELEMIR 690

BLAST of Lag0035312 vs. TAIR 10
Match: AT1G16160.1 (wall associated kinase-like 5 )

HSP 1 Score: 332.0 bits (850), Expect = 9.1e-91
Identity = 162/294 (55.10%), Postives = 222/294 (75.51%), Query Frame = 0

Query: 244 FILEELEQATDNYSQSRFLGQGGFGTVYKGILCDNTLVAIKRSKQIEENWIEQFINEVIV 303
           F  EEL++ATDN+S  R LG+G  GTVYKG++ D  ++A+KRSK ++E+ +E+FINE+I+
Sbjct: 400 FSSEELKKATDNFSVKRVLGKGSQGTVYKGMMVDGKIIAVKRSKVVDEDKLEKFINEIIL 459

Query: 304 LSQINHRNIVRLLGCCLETEFPLLVYEFVSNGTLSHHIHREDPQESPSLSWENRLNIASE 363
           LSQINHRNIV+L+GCCLETE P+LVYE++ NG +   +H  D  +  +++WE RL IA E
Sbjct: 460 LSQINHRNIVKLIGCCLETEVPILVYEYIPNGDMFKRLH--DESDDYAMTWEVRLRIAIE 519

Query: 364 VAGAISYMHSAASIPIFHRDIKSLNILLDDNFSAKVSDFGTSRFVSCDQSHLTTKVLGTF 423
           +AGA++YMHSAAS PI+HRDIK+ NILLD+ + AKVSDFGTSR V+ DQ+HLTT V GTF
Sbjct: 520 IAGALTYMHSAASFPIYHRDIKTTNILLDEKYGAKVSDFGTSRSVTIDQTHLTTMVAGTF 579

Query: 424 GYIDPEYFRSSQYTEKSDVYSFGVLLVELLTRKQPVT-FPKNGGGNLLDYFISLEMTNRL 483
           GY+DPEYF SSQYT+KSDVYSFGV+LVEL+T ++P++      G  L  +F+     NR+
Sbjct: 580 GYMDPEYFLSSQYTDKSDVYSFGVVLVELITGEKPLSRIRSEEGRGLATHFLEAMKENRV 639

Query: 484 VEILDVTVAKEEMREVVNNVAILAIKCLRSNGRERPTMKQVYLELEGLR-RPQD 536
           ++I+D+ + +E   + +  VA LA KCL   G +RP M++  LELE +R  P+D
Sbjct: 640 IDIIDIRIKEESKLDQLMAVAKLARKCLSRKGIKRPNMREASLELERIRSSPED 691

BLAST of Lag0035312 vs. TAIR 10
Match: AT1G69730.1 (Wall-associated kinase family protein )

HSP 1 Score: 330.1 bits (845), Expect = 3.5e-90
Identity = 164/314 (52.23%), Postives = 227/314 (72.29%), Query Frame = 0

Query: 244 FILEELEQATDNYSQSRFLGQGGFGTVYKGILCDNTLVAIKRSKQIEENWIEQFINEVIV 303
           F   ELE+AT+N+S +R LGQGG GTVYKG+L D  +VA+K+SK ++E+ +E+FINEV++
Sbjct: 435 FSSRELEKATENFSSNRILGQGGQGTVYKGMLVDGRIVAVKKSKVVDEDKLEEFINEVVI 494

Query: 304 LSQINHRNIVRLLGCCLETEFPLLVYEFVSNGTLSHHIHREDPQESPSLSWENRLNIASE 363
           LSQINHRNIV+LLGCCLET+ P+LVYEF+ NG L  H+H ++  E+   +W  RL IA +
Sbjct: 495 LSQINHRNIVKLLGCCLETKVPVLVYEFIPNGNLFEHLH-DEFDENIMATWNIRLRIAID 554

Query: 364 VAGAISYMHSAASIPIFHRDIKSLNILLDDNFSAKVSDFGTSRFVSCDQSHLTTKVLGTF 423
           +AGA+SY+HS+AS PI+HRD+KS NI+LD+ + AKVSDFGTSR V+ D +HLTT V GT 
Sbjct: 555 IAGALSYLHSSASSPIYHRDVKSTNIMLDEKYRAKVSDFGTSRTVTVDHTHLTTVVSGTV 614

Query: 424 GYIDPEYFRSSQYTEKSDVYSFGVLLVELLTRKQPVTFPKN-GGGNLLDYFISLEMTNRL 483
           GY+DPEYF+SSQ+T+KSDVYSFGV+LVEL+T ++ ++F ++     L  YFI     N+L
Sbjct: 615 GYMDPEYFQSSQFTDKSDVYSFGVVLVELITGEKSISFLRSQENRTLATYFILAMKENKL 674

Query: 484 VEILDVTVAKEEMREVVNNVAILAIKCLRSNGRERPTMKQVYLELEGLRRP------QDC 543
            +I+D  +    M   V   A +A KCL   GR+RP+M++V +EL+ +R P      Q+C
Sbjct: 675 FDIIDARIRDGCMLSQVTATAKVARKCLNLKGRKRPSMREVSMELDSIRMPCGDMQLQEC 734

Query: 544 LGIAEESHSRTFSL 551
           +   EE   +   L
Sbjct: 735 VSENEEGEEQNKGL 747

BLAST of Lag0035312 vs. TAIR 10
Match: AT4G31100.1 (wall-associated kinase, putative )

HSP 1 Score: 327.8 bits (839), Expect = 1.7e-89
Identity = 164/295 (55.59%), Postives = 218/295 (73.90%), Query Frame = 0

Query: 244 FILEELEQATDNYSQSRFLGQGGFGTVYKGILCDNTLVAIKRSKQIEENWIEQFINEVIV 303
           F  +ELE+AT+N+S++R LG GG GTVYKG+L D   VA+K+SK I+E+ +++FINEV++
Sbjct: 432 FTSKELEKATENFSENRVLGHGGQGTVYKGMLVDGRTVAVKKSKVIDEDKLQEFINEVVI 491

Query: 304 LSQINHRNIVRLLGCCLETEFPLLVYEFVSNGTLSHHIHREDPQESPSLSWENRLNIASE 363
           LSQINHR++V+LLGCCLETE P+LVYEF+ NG L  HIH E+  +  ++ W  RL IA +
Sbjct: 492 LSQINHRHVVKLLGCCLETEVPILVYEFIINGNLFKHIHEEEADDY-TMIWGMRLRIAVD 551

Query: 364 VAGAISYMHSAASIPIFHRDIKSLNILLDDNFSAKVSDFGTSRFVSCDQSHLTTKVLGTF 423
           +AGA+SY+HSAAS PI+HRDIKS NILLD+ + AKV+DFGTSR V+ DQ+H TT + GT 
Sbjct: 552 IAGALSYLHSAASSPIYHRDIKSTNILLDEKYRAKVADFGTSRSVTIDQTHWTTVISGTV 611

Query: 424 GYIDPEYFRSSQYTEKSDVYSFGVLLVELLTRKQPVTFPKNGGG--NLLDYFISLEMTNR 483
           GY+DPEY+RSSQYTEKSDVYSFGV+L EL+T  +PV   +N      L ++F       R
Sbjct: 612 GYVDPEYYRSSQYTEKSDVYSFGVILAELITGDKPVIMVQNTQEIIALAEHFRVAMKERR 671

Query: 484 LVEILDVTVAKEEMREVVNNVAILAIKCLRSNGRERPTMKQVYLELEGL-RRPQD 536
           L +I+D  +  +   E V  VA LA+KCL S GR RP M++V+ ELE +   P+D
Sbjct: 672 LSDIMDARIRDDSKPEQVMAVANLAMKCLSSRGRNRPNMREVFTELERICTSPED 725

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152076.16.1e-19861.49wall-associated receptor kinase-like 1 [Momordica charantia][more]
XP_022152101.16.6e-19260.88wall-associated receptor kinase-like 1 [Momordica charantia][more]
XP_022152099.14.6e-18558.75putative wall-associated receptor kinase-like 16 isoform X1 [Momordica charantia... [more]
XP_022961349.11.1e-16754.12wall-associated receptor kinase-like 2 [Cucurbita moschata][more]
XP_022987096.11.5e-15951.88wall-associated receptor kinase-like 2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9S9M51.6e-9256.48Wall-associated receptor kinase-like 1 OS=Arabidopsis thaliana OX=3702 GN=WAKL1 ... [more]
Q7X8C51.5e-9058.08Wall-associated receptor kinase-like 2 OS=Arabidopsis thaliana OX=3702 GN=WAKL2 ... [more]
Q9S9M33.4e-9053.92Wall-associated receptor kinase-like 3 OS=Arabidopsis thaliana OX=3702 GN=WAKL3 ... [more]
Q9S9M11.3e-8955.10Wall-associated receptor kinase-like 5 OS=Arabidopsis thaliana OX=3702 GN=WAKL5 ... [more]
Q9C9L54.9e-8952.23Wall-associated receptor kinase-like 9 OS=Arabidopsis thaliana OX=3702 GN=WAKL9 ... [more]
Match NameE-valueIdentityDescription
A0A6J1DGJ33.0e-19861.49wall-associated receptor kinase-like 1 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1DGL83.2e-19260.88wall-associated receptor kinase-like 1 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1DF112.2e-18558.75putative wall-associated receptor kinase-like 16 isoform X1 OS=Momordica charant... [more]
A0A6J1HDS35.5e-16854.12wall-associated receptor kinase-like 2 OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
A0A6J1JD617.2e-16051.88wall-associated receptor kinase-like 2 OS=Cucurbita maxima OX=3661 GN=LOC1114847... [more]
Match NameE-valueIdentityDescription
AT1G16120.11.2e-9356.48wall associated kinase-like 1 [more]
AT1G16130.11.1e-9158.08wall associated kinase-like 2 [more]
AT1G16160.19.1e-9155.10wall associated kinase-like 5 [more]
AT1G69730.13.5e-9052.23Wall-associated kinase family protein [more]
AT4G31100.11.7e-8955.59wall-associated kinase, putative [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 256..530
e-value: 4.1E-36
score: 136.0
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 256..523
e-value: 1.4E-50
score: 172.1
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 256..530
score: 38.859325
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 332..546
e-value: 2.5E-57
score: 195.5
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 228..331
e-value: 9.1E-31
score: 108.1
NoneNo IPR availablePANTHERPTHR27005:SF181LYSM TYPE RECEPTOR KINASEcoord: 29..219
NoneNo IPR availablePANTHERPTHR27005WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21coord: 244..546
coord: 29..219
NoneNo IPR availablePANTHERPTHR27005:SF181LYSM TYPE RECEPTOR KINASEcoord: 244..546
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 262..530
e-value: 1.91535E-95
score: 290.328
IPR018392LysM domainPFAMPF01476LysMcoord: 173..216
e-value: 0.045
score: 13.8
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 379..391
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 262..284
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 237..527

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0035312.1Lag0035312.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity