Lag0035265 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0035265
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	chr3: 17696732 .. 17697580 (-)
RNA-Seq Expression	Lag0035265
Synteny	Lag0035265

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGACTCTATGATCCCAACAGAGCTTCCCTCGACGCTCTTAGAGGTTCCAACATTGAACTCATGCTTGGCCTTCCCAACCCTGATCTCCAAAGAATCGCTTCAAGTCAGGCCGAAGCAAATTCATGGGTTCAAAACAACGTTAGAAACTATCCTAACGTTAGATTCAAGTACATTGTTGTTGGCAATGAAGTCCAACCCTCTAGTCCTACTGCTGCATTCGTTGTCCCAGCAATGCGAAACATCCAAACTGCAATTTCCAATGCTGGTTTGGCTAATCAAATCAAAGTCTCCACCGCTATTGACACTGGCGTCCTCGGCGAGTCTTTCCCTCCTTCTAAAGGCTCCTTTAGAAATGATGCCCTATCGCTACTCAACCCCATCATTCGTTTTCTCAATGAAAATCGCTCACCGTTGCTCGTCAACTTGTACCCTTACTTCAGTTACATTGGTAACCCAAGCGCCATTCGTTTGGACTATGCCCTCTTCACAGCACCATCAGTGGTGGTCACCGATGGCCAGCTTCGTTACCAAAATCTGTTTGATGCAATTCTTGATGCTGTTTACGCAGCATTGGAGAAGGCCAATGGAGGAAGTTTGGAGATTGTGATATCAGAGAGTGGATGGCCATCGGCAGGTGGTGCAGCAACGTCGATTGATAATGCACGAACATACAACAATAATCTGATTCAACATGTCAAGAGAGGGACTCCCAGGCGCTCTGGTAGACCGATTGAAACTTATATTTTTGCCATGTTTGATGAGAACCAAAAGAGTCCTGAGTTCGAGAAGCATTTTGGTCTTTTCTCTCCAAACAAGCAGCTTAAATATCCAGTCAACTTCAATTGA

mRNA sequence

ATGAGACTCTATGATCCCAACAGAGCTTCCCTCGACGCTCTTAGAGGTTCCAACATTGAACTCATGCTTGGCCTTCCCAACCCTGATCTCCAAAGAATCGCTTCAAGTCAGGCCGAAGCAAATTCATGGGTTCAAAACAACGTTAGAAACTATCCTAACGTTAGATTCAAGTACATTGTTGTTGGCAATGAAGTCCAACCCTCTAGTCCTACTGCTGCATTCGTTGTCCCAGCAATGCGAAACATCCAAACTGCAATTTCCAATGCTGGTTTGGCTAATCAAATCAAAGTCTCCACCGCTATTGACACTGGCGTCCTCGGCGAGTCTTTCCCTCCTTCTAAAGGCTCCTTTAGAAATGATGCCCTATCGCTACTCAACCCCATCATTCGTTTTCTCAATGAAAATCGCTCACCGTTGCTCGTCAACTTGTACCCTTACTTCAGTTACATTGGTAACCCAAGCGCCATTCGTTTGGACTATGCCCTCTTCACAGCACCATCAGTGGTGGTCACCGATGGCCAGCTTCGTTACCAAAATCTGTTTGATGCAATTCTTGATGCTGTTTACGCAGCATTGGAGAAGGCCAATGGAGGAAGTTTGGAGATTGTGATATCAGAGAGTGGATGGCCATCGGCAGGTGGTGCAGCAACGTCGATTGATAATGCACGAACATACAACAATAATCTGATTCAACATGTCAAGAGAGGGACTCCCAGGCGCTCTGGTAGACCGATTGAAACTTATATTTTTGCCATGTTTGATGAGAACCAAAAGAGTCCTGAGTTCGAGAAGCATTTTGGTCTTTTCTCTCCAAACAAGCAGCTTAAATATCCAGTCAACTTCAATTGA

Coding sequence (CDS)

ATGAGACTCTATGATCCCAACAGAGCTTCCCTCGACGCTCTTAGAGGTTCCAACATTGAACTCATGCTTGGCCTTCCCAACCCTGATCTCCAAAGAATCGCTTCAAGTCAGGCCGAAGCAAATTCATGGGTTCAAAACAACGTTAGAAACTATCCTAACGTTAGATTCAAGTACATTGTTGTTGGCAATGAAGTCCAACCCTCTAGTCCTACTGCTGCATTCGTTGTCCCAGCAATGCGAAACATCCAAACTGCAATTTCCAATGCTGGTTTGGCTAATCAAATCAAAGTCTCCACCGCTATTGACACTGGCGTCCTCGGCGAGTCTTTCCCTCCTTCTAAAGGCTCCTTTAGAAATGATGCCCTATCGCTACTCAACCCCATCATTCGTTTTCTCAATGAAAATCGCTCACCGTTGCTCGTCAACTTGTACCCTTACTTCAGTTACATTGGTAACCCAAGCGCCATTCGTTTGGACTATGCCCTCTTCACAGCACCATCAGTGGTGGTCACCGATGGCCAGCTTCGTTACCAAAATCTGTTTGATGCAATTCTTGATGCTGTTTACGCAGCATTGGAGAAGGCCAATGGAGGAAGTTTGGAGATTGTGATATCAGAGAGTGGATGGCCATCGGCAGGTGGTGCAGCAACGTCGATTGATAATGCACGAACATACAACAATAATCTGATTCAACATGTCAAGAGAGGGACTCCCAGGCGCTCTGGTAGACCGATTGAAACTTATATTTTTGCCATGTTTGATGAGAACCAAAAGAGTCCTGAGTTCGAGAAGCATTTTGGTCTTTTCTCTCCAAACAAGCAGCTTAAATATCCAGTCAACTTCAATTGA

Protein sequence

MRLYDPNRASLDALRGSNIELMLGLPNPDLQRIASSQAEANSWVQNNVRNYPNVRFKYIVVGNEVQPSSPTAAFVVPAMRNIQTAISNAGLANQIKVSTAIDTGVLGESFPPSKGSFRNDALSLLNPIIRFLNENRSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQLRYQNLFDAILDAVYAALEKANGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPRRSGRPIETYIFAMFDENQKSPEFEKHFGLFSPNKQLKYPVNFN

Homology

[Close]

BLAST of Lag0035265 vs. NCBI nr
Match: XP_038876764.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Benincasa hispida])

HSP 1 Score: 479.2 bits (1232), Expect = 2.5e-131
Identity = 239/282 (84.75%), Postives = 254/282 (90.07%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNIELMLGLPNPDLQRIASSQAEANSWVQNNVRNYPNVRFKYIV 60
           MRLYDPNRASLDALRGSNIELMLGLPN DLQRIASSQ+EAN+WVQNNV+++ NVRFKYI 
Sbjct: 56  MRLYDPNRASLDALRGSNIELMLGLPNSDLQRIASSQSEANAWVQNNVKSFGNVRFKYIA 115

Query: 61  VGNEVQPSSPTAAFVVPAMRNIQTAISNAGLANQIKVSTAIDTGVLGESFPPSKGSFRND 120
           VGNEVQPSS  A FVVPAM+NIQTA+SN GLANQIKVSTAIDTG+LG SFPPS G+F N 
Sbjct: 116 VGNEVQPSSSAAQFVVPAMQNIQTALSNGGLANQIKVSTAIDTGLLGVSFPPSSGAFNNQ 175

Query: 121 ALSLLNPIIRFLNENRSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQLRYQNL 180
           A+ LLNPIIRFLN NRSPLLVNLYP+FSYI NP  IRLDYALFTA S VVTDGQLRYQNL
Sbjct: 176 AMPLLNPIIRFLNANRSPLLVNLYPFFSYISNPRDIRLDYALFTAKSTVVTDGQLRYQNL 235

Query: 181 FDAILDAVYAALEKANGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPRR 240
           FDAI+DA+YAALEKA G SLEIV+SESGWPSAGG A S +NARTYNNNLIQHVKRGTPRR
Sbjct: 236 FDAIVDAIYAALEKAGGSSLEIVVSESGWPSAGGTAASTENARTYNNNLIQHVKRGTPRR 295

Query: 241 SGRPIETYIFAMFDENQKSPEFEKHFGLFSPNKQLKYPVNFN 283
             RPIETYIFAMFDENQKSPEFEKHFGLF PNKQ KY VNFN
Sbjct: 296 PQRPIETYIFAMFDENQKSPEFEKHFGLFFPNKQPKYAVNFN 337

[Close]

BLAST of Lag0035265 vs. NCBI nr
Match: TYK19849.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo var. makuwa])

HSP 1 Score: 474.9 bits (1221), Expect = 4.7e-130
Identity = 233/282 (82.62%), Postives = 252/282 (89.36%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNIELMLGLPNPDLQRIASSQAEANSWVQNNVRNYPNVRFKYIV 60
           MRLYDPNR SLDALRGS IELMLGLPNPDLQRIASSQAEAN+WVQNN++NY NVRFKYI 
Sbjct: 23  MRLYDPNRGSLDALRGSTIELMLGLPNPDLQRIASSQAEANAWVQNNIKNYQNVRFKYIA 82

Query: 61  VGNEVQPSSPTAAFVVPAMRNIQTAISNAGLANQIKVSTAIDTGVLGESFPPSKGSFRND 120
           VGNEVQPSS  A+FVVPAMRNIQTA++NAGLAN+IKVSTAIDT +L  S+PPS G+ +N+
Sbjct: 83  VGNEVQPSSSAASFVVPAMRNIQTALNNAGLANRIKVSTAIDTSILAMSYPPSSGTIKNE 142

Query: 121 ALSLLNPIIRFLNENRSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQLRYQNL 180
            +  ++ +IRFL +N SPLLVNLYPYFSYIGNP  IRLDYALFTAPS VV DGQ RYQNL
Sbjct: 143 DMPFMSSVIRFLKDNGSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQFRYQNL 202

Query: 181 FDAILDAVYAALEKANGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPRR 240
           FDAILDAVYAALEK  GG+LEIVISESGWPSAGG A SIDNARTY NNLIQHVKRGTPRR
Sbjct: 203 FDAILDAVYAALEKVGGGNLEIVISESGWPSAGGTAASIDNARTYINNLIQHVKRGTPRR 262

Query: 241 SGRPIETYIFAMFDENQKSPEFEKHFGLFSPNKQLKYPVNFN 283
            GRPIETYIFAMFDENQK+PEFEKHFGLF PNKQ KYPVNFN
Sbjct: 263 PGRPIETYIFAMFDENQKNPEFEKHFGLFFPNKQSKYPVNFN 304

[Close]

BLAST of Lag0035265 vs. NCBI nr
Match: XP_022987104.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucurbita maxima])

HSP 1 Score: 473.0 bits (1216), Expect = 1.8e-129
Identity = 232/282 (82.27%), Postives = 256/282 (90.78%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNIELMLGLPNPDLQRIASSQAEANSWVQNNVRNYPNVRFKYIV 60
           MRLY PNR SL AL GSNIEL+L +PNPDLQRIASSQAEAN+WVQNN+R++PNVRFKYI 
Sbjct: 56  MRLYGPNRDSLGALSGSNIELILDVPNPDLQRIASSQAEANTWVQNNIRSFPNVRFKYIS 115

Query: 61  VGNEVQPSSPTAAFVVPAMRNIQTAISNAGLANQIKVSTAIDTGVLGESFPPSKGSFRND 120
           VGNEVQPSS  A FVVPAMRNIQTA++N GL+++IKVST+IDTGV+G SFPPS G+F+++
Sbjct: 116 VGNEVQPSSSNAQFVVPAMRNIQTALANVGLSSRIKVSTSIDTGVIGTSFPPSNGAFKSE 175

Query: 121 ALSLLNPIIRFLNENRSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQLRYQNL 180
           A+ LLNPIIRFLNEN SPLLVNLYPYFSYIGN   IRLDYALFTAPS VVTDGQ RYQNL
Sbjct: 176 AMPLLNPIIRFLNENSSPLLVNLYPYFSYIGNMRDIRLDYALFTAPSTVVTDGQFRYQNL 235

Query: 181 FDAILDAVYAALEKANGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPRR 240
           FDAILDA YAALEKA G +LEIV+SESGWP+AGGAATSI NARTY NNLIQHVKRGTPRR
Sbjct: 236 FDAILDATYAALEKAGGRNLEIVVSESGWPTAGGAATSISNARTYINNLIQHVKRGTPRR 295

Query: 241 SGRPIETYIFAMFDENQKSPEFEKHFGLFSPNKQLKYPVNFN 283
            GRPIE YIFAMFDENQK+PEFEKHFGLF+PNKQLKYP+NFN
Sbjct: 296 PGRPIEAYIFAMFDENQKNPEFEKHFGLFTPNKQLKYPINFN 337

[Close]

BLAST of Lag0035265 vs. NCBI nr
Match: KAG6590084.1 (Glucan endo-1,3-beta-glucosidase, basic isoform, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 470.7 bits (1210), Expect = 8.9e-129
Identity = 232/282 (82.27%), Postives = 255/282 (90.43%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNIELMLGLPNPDLQRIASSQAEANSWVQNNVRNYPNVRFKYIV 60
           MRLY PNR SL+AL GSNIEL+L +PNPDLQRIASSQAEAN+WVQNN+R+ PNVRFKYI 
Sbjct: 23  MRLYGPNRDSLNALSGSNIELILDVPNPDLQRIASSQAEANTWVQNNIRSLPNVRFKYIA 82

Query: 61  VGNEVQPSSPTAAFVVPAMRNIQTAISNAGLANQIKVSTAIDTGVLGESFPPSKGSFRND 120
           VGNEVQ SS  A FVVPAMRNIQTA++N GL ++IKVST+IDTGVLG SFPPS G+F+++
Sbjct: 83  VGNEVQSSSSNAQFVVPAMRNIQTALANVGLNSRIKVSTSIDTGVLGTSFPPSNGAFKSE 142

Query: 121 ALSLLNPIIRFLNENRSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQLRYQNL 180
           A+ LLNPIIRFLNEN SPLLVNLYPYFSYIGN   IRLDYALFTAPS VVTDGQ RYQNL
Sbjct: 143 AMPLLNPIIRFLNENGSPLLVNLYPYFSYIGNMRDIRLDYALFTAPSTVVTDGQFRYQNL 202

Query: 181 FDAILDAVYAALEKANGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPRR 240
           FDAILDA YAALEKA G ++EIV+SESGWP+AGG ATSIDNARTY NNLIQHVKRGTPRR
Sbjct: 203 FDAILDATYAALEKAGGRNVEIVVSESGWPTAGGTATSIDNARTYINNLIQHVKRGTPRR 262

Query: 241 SGRPIETYIFAMFDENQKSPEFEKHFGLFSPNKQLKYPVNFN 283
            G+PIETYIFAMFDENQK+PEFEKHFGLFSPNKQLKYP+NFN
Sbjct: 263 PGKPIETYIFAMFDENQKNPEFEKHFGLFSPNKQLKYPINFN 304

[Close]

BLAST of Lag0035265 vs. NCBI nr
Match: KAG7023754.1 (Glucan endo-1,3-beta-glucosidase, basic isoform, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 469.5 bits (1207), Expect = 2.0e-128
Identity = 231/282 (81.91%), Postives = 254/282 (90.07%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNIELMLGLPNPDLQRIASSQAEANSWVQNNVRNYPNVRFKYIV 60
           MRLY PNR SL+AL GSNIEL+L +PNPDLQRIASSQAEAN+WVQNN+R+ PNVRFKYI 
Sbjct: 208 MRLYGPNRDSLNALSGSNIELILDVPNPDLQRIASSQAEANTWVQNNIRSLPNVRFKYIA 267

Query: 61  VGNEVQPSSPTAAFVVPAMRNIQTAISNAGLANQIKVSTAIDTGVLGESFPPSKGSFRND 120
           VGNEVQ SS  A FVVPAMRNIQTA+++ GL  +IKVSTAIDTGVLG SFPPS G+F+++
Sbjct: 268 VGNEVQSSSSNAQFVVPAMRNIQTALASVGLGGRIKVSTAIDTGVLGTSFPPSNGAFKSE 327

Query: 121 ALSLLNPIIRFLNENRSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQLRYQNL 180
           A+ LLNPIIRFLNEN SPLLVNLYPYFSYIGN   IRLDYALFTAPS VVTDGQ RYQNL
Sbjct: 328 AMPLLNPIIRFLNENGSPLLVNLYPYFSYIGNMRDIRLDYALFTAPSTVVTDGQFRYQNL 387

Query: 181 FDAILDAVYAALEKANGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPRR 240
           FDAILDA YAALEKA G ++EIV+SESGWP+AGG ATSIDNARTY NNLIQHVKRGTPRR
Sbjct: 388 FDAILDATYAALEKAGGRNVEIVVSESGWPTAGGTATSIDNARTYINNLIQHVKRGTPRR 447

Query: 241 SGRPIETYIFAMFDENQKSPEFEKHFGLFSPNKQLKYPVNFN 283
            G+PIETYIFAMFDENQK+PEFEKHFGLFSPNKQ+KYP+NFN
Sbjct: 448 PGKPIETYIFAMFDENQKNPEFEKHFGLFSPNKQIKYPINFN 489

[Close]

BLAST of Lag0035265 vs. ExPASy Swiss-Prot
Match: P52408 (Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS1 PE=3 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 4.7e-117
Identity = 211/282 (74.82%), Postives = 243/282 (86.17%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNIELMLGLPNPDLQRIASSQAEANSWVQNNVRNYPNVRFKYIV 60
           MRLYDPN A+L+ALRGSNI+L+LG+PN +LQ IA SQA AN+WVQNNVRNY NV+FKYI 
Sbjct: 69  MRLYDPNPAALEALRGSNIKLLLGVPNENLQYIALSQANANAWVQNNVRNYANVKFKYIA 128

Query: 61  VGNEVQPSSPTAAFVVPAMRNIQTAISNAGLANQIKVSTAIDTGVLGESFPPSKGSFRND 120
           VGNEV+PS   A F+VPAMRNIQ AIS AGLA +IKVSTAIDTGVLGE+FPPS GSF+++
Sbjct: 129 VGNEVKPSDSFAQFLVPAMRNIQEAISLAGLAKKIKVSTAIDTGVLGETFPPSIGSFKSE 188

Query: 121 ALSLLNPIIRFLNENRSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQLRYQNL 180
             +LL PIIRFL  ++SPLLVNLYPYF+Y GN   IRLDYALFTAPSVVV DG   Y+NL
Sbjct: 189 YNALLYPIIRFLVSHQSPLLVNLYPYFAYSGNTQDIRLDYALFTAPSVVVQDGNFGYRNL 248

Query: 181 FDAILDAVYAALEKANGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPRR 240
           FDA+LD VYAALEKA GGSL++VISE+GWPSA G AT+IDNART+ +NLIQHVK GTPRR
Sbjct: 249 FDAMLDGVYAALEKAGGGSLKVVISETGWPSAAGTATTIDNARTFISNLIQHVKEGTPRR 308

Query: 241 SGRPIETYIFAMFDENQKSPEFEKHFGLFSPNKQLKYPVNFN 283
            GRPIETYIFAMFDEN+K+PE EKH+GLFSP KQ KY ++FN
Sbjct: 309 PGRPIETYIFAMFDENRKTPELEKHWGLFSPTKQPKYQISFN 350

[Close]

BLAST of Lag0035265 vs. ExPASy Swiss-Prot
Match: P07979 (Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3)

HSP 1 Score: 383.6 bits (984), Expect = 1.9e-105
Identity = 189/285 (66.32%), Postives = 232/285 (81.40%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNIELMLGLPNPDLQRIASSQAEANSWVQNNVRNY-PNVRFKYI 60
           MRLYDPN+A+L ALRGSNIE+MLG+PN DLQ IA++ + AN+WVQ NVRN+ P V+F+YI
Sbjct: 62  MRLYDPNQAALQALRGSNIEVMLGVPNSDLQNIAANPSNANNWVQRNVRNFWPAVKFRYI 121

Query: 61  VVGNEVQPSSPTAA---FVVPAMRNIQTAISNAGLANQIKVSTAIDTGVLGESFPPSKGS 120
            VGNEV P + T++   +++PAMRNI+ AIS+AGL N IKVS+++D  ++G SFPPS+GS
Sbjct: 122 AVGNEVSPVTGTSSLTRYLLPAMRNIRNAISSAGLQNNIKVSSSVDMTLIGNSFPPSQGS 181

Query: 121 FRNDALSLLNPIIRFLNENRSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQLR 180
           FRND  S ++PII F+    SPLLVN+YPYFSY GNP  I L YALFTAP+VVV DG L 
Sbjct: 182 FRNDVRSFIDPIIGFVRRINSPLLVNIYPYFSYAGNPRDISLPYALFTAPNVVVQDGSLG 241

Query: 181 YQNLFDAILDAVYAALEKANGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRG 240
           Y+NLFDA+ DAVYAAL +A GGS+EIV+SESGWPSAG  A + +NA TY  NLIQHVKRG
Sbjct: 242 YRNLFDAMSDAVYAALSRAGGGSIEIVVSESGWPSAGAFAATTNNAATYYKNLIQHVKRG 301

Query: 241 TPRRSGRPIETYIFAMFDENQKSPEFEKHFGLFSPNKQLKYPVNF 282
           +PRR  + IETY+FAMFDEN K+PE EKHFGLFSPNKQ KYP++F
Sbjct: 302 SPRRPNKVIETYLFAMFDENNKNPELEKHFGLFSPNKQPKYPLSF 346

[Close]

BLAST of Lag0035265 vs. ExPASy Swiss-Prot
Match: Q03773 (Glucan endo-1,3-beta-glucosidase OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 5.4e-105
Identity = 190/285 (66.67%), Postives = 231/285 (81.05%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNIELMLGLPNPDLQRIASSQAEANSWVQNNVRNYP-NVRFKYI 60
           MR+Y P+   L+ALRGSNIEL+L +PN +L+ +ASSQ  AN WVQ+N++NY  NVRF+Y+
Sbjct: 63  MRIYGPSPEVLEALRGSNIELLLDIPNDNLRNLASSQDNANKWVQDNIKNYANNVRFRYV 122

Query: 61  VVGNEVQPSSPTAAFVVPAMRNIQTAISNAGLANQIKVSTAIDTGVLGESFPPSKGSFRN 120
            VGNEV+P    A F+VPA+ NIQ AISNAGL NQ+KVSTAIDTG L ESFPPSKGSF++
Sbjct: 123 SVGNEVKPEHSFAQFLVPALENIQRAISNAGLGNQVKVSTAIDTGALAESFPPSKGSFKS 182

Query: 121 DAL-SLLNPIIRFLNENRSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQLRYQ 180
           D   + L+ +IRFL  N +PL+VN+Y YF+Y  NP  I LDYALF +PSVVV DG L Y+
Sbjct: 183 DYRGAYLDGVIRFLVNNNAPLMVNVYSYFAYTANPKDISLDYALFRSPSVVVQDGSLGYR 242

Query: 181 NLFDAILDAVYAALEKANGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTP 240
           NLFDA +DAVYAALEKA GGSL IV+SESGWPS+GG ATS+DNARTYN NL+++VK+GTP
Sbjct: 243 NLFDASVDAVYAALEKAGGGSLNIVVSESGWPSSGGTATSLDNARTYNTNLVRNVKQGTP 302

Query: 241 RRSGRPIETYIFAMFDENQKSPEFEKHFGLFSP-NKQLKYPVNFN 283
           +R G P+ETY+FAMFDENQK PEFEK +GLFSP  KQ KY +NFN
Sbjct: 303 KRPGAPLETYVFAMFDENQKQPEFEKFWGLFSPITKQPKYSINFN 347

[Close]

BLAST of Lag0035265 vs. ExPASy Swiss-Prot
Match: F4J270 (Probable glucan endo-1,3-beta-glucosidase BG3 OS=Arabidopsis thaliana OX=3702 GN=BG3 PE=2 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 1.7e-103
Identity = 197/284 (69.37%), Postives = 228/284 (80.28%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNIELMLGLPNPDLQRIASSQAEANSWVQNNVRNYPNVRFKYIV 60
           MRLYDPN+ +L+ALRGSNIEL+L +PNPDLQR+ASSQAEA++WV+NNVRNY NV F+YI 
Sbjct: 64  MRLYDPNQETLNALRGSNIELVLDVPNPDLQRLASSQAEADTWVRNNVRNYANVTFRYIS 123

Query: 61  VGNEVQPSSPTAAFVVPAMRNIQTAISNAGLANQIKVSTAIDT-GVLGESFPPSKGSFRN 120
           VGNEVQPS   A+FV+PAM+NI+ A+S+ G    IKVSTAIDT G+ G  FPPS G+F  
Sbjct: 124 VGNEVQPSDQAASFVLPAMQNIERAVSSLG----IKVSTAIDTRGISG--FPPSSGTFTP 183

Query: 121 DALSLLNPIIRFLNENRSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQLRYQN 180
           +  S + P+I FL+  +SPLLVN YPYFSY GN   IRLDY LFTAPS VV DGQ +Y+N
Sbjct: 184 EFRSFIAPVISFLSSKQSPLLVNNYPYFSYTGNMRDIRLDYTLFTAPSTVVNDGQNQYRN 243

Query: 181 LFDAILDAVYAALEKANGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPR 240
           LF AILD VYA+LEKA GGSLEIV+SESGWP+AGGAAT +DNARTY NNLIQ VK G+PR
Sbjct: 244 LFHAILDTVYASLEKAGGGSLEIVVSESGWPTAGGAATGVDNARTYVNNLIQTVKNGSPR 303

Query: 241 RSGRPIETYIFAMFDENQK-SPEFEKHFGLFSPNKQLKYPVNFN 283
           R GR  ETYIFAMFDEN K  PE EK +GLF PN Q KY VNFN
Sbjct: 304 RPGRATETYIFAMFDENSKQGPETEKFWGLFLPNLQPKYVVNFN 341

[Close]

BLAST of Lag0035265 vs. ExPASy Swiss-Prot
Match: P36401 (Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum OX=4097 PE=1 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 3.9e-103
Identity = 189/286 (66.08%), Postives = 230/286 (80.42%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNIELMLGLPNPDLQRIASSQAEANSWVQNNVRNYPNVRFKYIV 60
           MR+YDP++ +L+ALRGSNIELMLG+PNPDL+ +A+SQA A++WVQNNVRNY NV+F+YI 
Sbjct: 55  MRIYDPDQPTLEALRGSNIELMLGVPNPDLENVAASQANADTWVQNNVRNYGNVKFRYIA 114

Query: 61  VGNEVQPSSPTAAFV---VPAMRNIQTAISNAGLANQIKVSTAIDTGVLGESFPPSKGSF 120
           VGNEV P +  + +V   + AMRNIQTAIS AGL NQIKVSTAI+TG+  ++ PPS G F
Sbjct: 115 VGNEVSPLNENSKYVPVLLNAMRNIQTAISGAGLGNQIKVSTAIETGLTTDTSPPSNGRF 174

Query: 121 RNDALSLLNPIIRFLNENRSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQLRY 180
           ++D    + PII FL  NR+PLLVNLYPYF+ I N + I+L+YALFT+  VVV D    Y
Sbjct: 175 KDDVRQFIEPIINFLVTNRAPLLVNLYPYFA-IANNADIKLEYALFTSSEVVVNDNGRGY 234

Query: 181 QNLFDAILDAVYAALEKANGGSLEIVISESGWPSAG-GAATSIDNARTYNNNLIQHVKRG 240
           +NLFDAILDA Y+ALEKA+G SLEIV+SESGWPSAG G  TSIDNARTYNNNLI HVK G
Sbjct: 235 RNLFDAILDATYSALEKASGSSLEIVVSESGWPSAGAGQLTSIDNARTYNNNLISHVKGG 294

Query: 241 TPRRSGRPIETYIFAMFDENQKSPEFEKHFGLFSPNKQLKYPVNFN 283
           +P+R   PIETY+FA+FDE+QK PE EKHFGLFS N Q KY ++FN
Sbjct: 295 SPKRPSGPIETYVFALFDEDQKDPEIEKHFGLFSANMQPKYQISFN 339

[Close]

BLAST of Lag0035265 vs. ExPASy TrEMBL
Match: A0A5D3D8R0 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold811G00600 PE=3 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 2.3e-130
Identity = 233/282 (82.62%), Postives = 252/282 (89.36%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNIELMLGLPNPDLQRIASSQAEANSWVQNNVRNYPNVRFKYIV 60
           MRLYDPNR SLDALRGS IELMLGLPNPDLQRIASSQAEAN+WVQNN++NY NVRFKYI 
Sbjct: 23  MRLYDPNRGSLDALRGSTIELMLGLPNPDLQRIASSQAEANAWVQNNIKNYQNVRFKYIA 82

Query: 61  VGNEVQPSSPTAAFVVPAMRNIQTAISNAGLANQIKVSTAIDTGVLGESFPPSKGSFRND 120
           VGNEVQPSS  A+FVVPAMRNIQTA++NAGLAN+IKVSTAIDT +L  S+PPS G+ +N+
Sbjct: 83  VGNEVQPSSSAASFVVPAMRNIQTALNNAGLANRIKVSTAIDTSILAMSYPPSSGTIKNE 142

Query: 121 ALSLLNPIIRFLNENRSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQLRYQNL 180
            +  ++ +IRFL +N SPLLVNLYPYFSYIGNP  IRLDYALFTAPS VV DGQ RYQNL
Sbjct: 143 DMPFMSSVIRFLKDNGSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQFRYQNL 202

Query: 181 FDAILDAVYAALEKANGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPRR 240
           FDAILDAVYAALEK  GG+LEIVISESGWPSAGG A SIDNARTY NNLIQHVKRGTPRR
Sbjct: 203 FDAILDAVYAALEKVGGGNLEIVISESGWPSAGGTAASIDNARTYINNLIQHVKRGTPRR 262

Query: 241 SGRPIETYIFAMFDENQKSPEFEKHFGLFSPNKQLKYPVNFN 283
            GRPIETYIFAMFDENQK+PEFEKHFGLF PNKQ KYPVNFN
Sbjct: 263 PGRPIETYIFAMFDENQKNPEFEKHFGLFFPNKQSKYPVNFN 304

[Close]

BLAST of Lag0035265 vs. ExPASy TrEMBL
Match: A0A6J1JHX8 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484757 PE=3 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 8.6e-130
Identity = 232/282 (82.27%), Postives = 256/282 (90.78%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNIELMLGLPNPDLQRIASSQAEANSWVQNNVRNYPNVRFKYIV 60
           MRLY PNR SL AL GSNIEL+L +PNPDLQRIASSQAEAN+WVQNN+R++PNVRFKYI 
Sbjct: 56  MRLYGPNRDSLGALSGSNIELILDVPNPDLQRIASSQAEANTWVQNNIRSFPNVRFKYIS 115

Query: 61  VGNEVQPSSPTAAFVVPAMRNIQTAISNAGLANQIKVSTAIDTGVLGESFPPSKGSFRND 120
           VGNEVQPSS  A FVVPAMRNIQTA++N GL+++IKVST+IDTGV+G SFPPS G+F+++
Sbjct: 116 VGNEVQPSSSNAQFVVPAMRNIQTALANVGLSSRIKVSTSIDTGVIGTSFPPSNGAFKSE 175

Query: 121 ALSLLNPIIRFLNENRSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQLRYQNL 180
           A+ LLNPIIRFLNEN SPLLVNLYPYFSYIGN   IRLDYALFTAPS VVTDGQ RYQNL
Sbjct: 176 AMPLLNPIIRFLNENSSPLLVNLYPYFSYIGNMRDIRLDYALFTAPSTVVTDGQFRYQNL 235

Query: 181 FDAILDAVYAALEKANGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPRR 240
           FDAILDA YAALEKA G +LEIV+SESGWP+AGGAATSI NARTY NNLIQHVKRGTPRR
Sbjct: 236 FDAILDATYAALEKAGGRNLEIVVSESGWPTAGGAATSISNARTYINNLIQHVKRGTPRR 295

Query: 241 SGRPIETYIFAMFDENQKSPEFEKHFGLFSPNKQLKYPVNFN 283
            GRPIE YIFAMFDENQK+PEFEKHFGLF+PNKQLKYP+NFN
Sbjct: 296 PGRPIEAYIFAMFDENQKNPEFEKHFGLFTPNKQLKYPINFN 337

[Close]

BLAST of Lag0035265 vs. ExPASy TrEMBL
Match: A0A5A7TA86 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold270G001600 PE=3 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 1.2e-128
Identity = 232/283 (81.98%), Postives = 251/283 (88.69%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNIELMLGLPNPDLQRIASSQAEANSWVQNNVRNYPNVRFKYIV 60
           MRLYDPNR SLDALRGS IELMLGLPNPDLQRIASSQAEAN+WVQNN++NY NVRFKYI 
Sbjct: 23  MRLYDPNRGSLDALRGSTIELMLGLPNPDLQRIASSQAEANAWVQNNIKNYQNVRFKYIA 82

Query: 61  VGNEVQPSSPTAAFVVPAMRNIQTAISNAGLANQIKVSTAIDTGVLGESFPPSKGSFRND 120
           VGNEVQPSS  A+FVVPAMRNIQTA++NAGL N+IKVSTAIDT +L  S+PPS G+ +N+
Sbjct: 83  VGNEVQPSSSAASFVVPAMRNIQTALNNAGLVNRIKVSTAIDTSILAMSYPPSSGTIKNE 142

Query: 121 ALSLLNPIIRFLNENRSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQLRYQNL 180
            +  ++ +IRFL +N SPLLVNLYPYFSYIGNP  IRLDYALFTAPS VV DGQ RYQNL
Sbjct: 143 DMPFMSSVIRFLKDNGSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQFRYQNL 202

Query: 181 FDAILDAVYAALEKANGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPRR 240
           FDAILDAVYAALEK  GG+LEIVISESGWPSAGG A SIDNARTY NNLIQHVKRGTPRR
Sbjct: 203 FDAILDAVYAALEKVGGGNLEIVISESGWPSAGGTAASIDNARTYINNLIQHVKRGTPRR 262

Query: 241 -SGRPIETYIFAMFDENQKSPEFEKHFGLFSPNKQLKYPVNFN 283
             GRPIETYIFAMFDENQK+PEFEKHFGLF PNKQ KYPVNFN
Sbjct: 263 PGGRPIETYIFAMFDENQKNPEFEKHFGLFFPNKQSKYPVNFN 305

[Close]

BLAST of Lag0035265 vs. ExPASy TrEMBL
Match: A0A1S3B9H8 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487244 PE=3 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 1.2e-128
Identity = 232/283 (81.98%), Postives = 251/283 (88.69%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNIELMLGLPNPDLQRIASSQAEANSWVQNNVRNYPNVRFKYIV 60
           MRLYDPNR SLDALRGS IELMLGLPNPDLQRIASSQAEAN+WVQNN++NY NVRFKYI 
Sbjct: 57  MRLYDPNRGSLDALRGSTIELMLGLPNPDLQRIASSQAEANAWVQNNIKNYQNVRFKYIA 116

Query: 61  VGNEVQPSSPTAAFVVPAMRNIQTAISNAGLANQIKVSTAIDTGVLGESFPPSKGSFRND 120
           VGNEVQPSS  A+FVVPAMRNIQTA++NAGL N+IKVSTAIDT +L  S+PPS G+ +N+
Sbjct: 117 VGNEVQPSSSAASFVVPAMRNIQTALNNAGLVNRIKVSTAIDTSILAMSYPPSSGTIKNE 176

Query: 121 ALSLLNPIIRFLNENRSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQLRYQNL 180
            +  ++ +IRFL +N SPLLVNLYPYFSYIGNP  IRLDYALFTAPS VV DGQ RYQNL
Sbjct: 177 DMPFMSSVIRFLKDNGSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQFRYQNL 236

Query: 181 FDAILDAVYAALEKANGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPRR 240
           FDAILDAVYAALEK  GG+LEIVISESGWPSAGG A SIDNARTY NNLIQHVKRGTPRR
Sbjct: 237 FDAILDAVYAALEKVGGGNLEIVISESGWPSAGGTAASIDNARTYINNLIQHVKRGTPRR 296

Query: 241 -SGRPIETYIFAMFDENQKSPEFEKHFGLFSPNKQLKYPVNFN 283
             GRPIETYIFAMFDENQK+PEFEKHFGLF PNKQ KYPVNFN
Sbjct: 297 PGGRPIETYIFAMFDENQKNPEFEKHFGLFFPNKQSKYPVNFN 339

[Close]

BLAST of Lag0035265 vs. ExPASy TrEMBL
Match: A0A6J1CAZ7 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111010014 PE=3 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 2.4e-127
Identity = 234/283 (82.69%), Postives = 249/283 (87.99%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNIELMLGLPNPDLQRIASSQAEANSWVQNNVRNYPNVRFKYIV 60
           MRLYDPNRASLDAL+ SNIEL+LGLPNPDLQRIASSQ EAN+WVQNNVRN+ NVRFKYI 
Sbjct: 56  MRLYDPNRASLDALKXSNIELILGLPNPDLQRIASSQGEANAWVQNNVRNFANVRFKYIA 115

Query: 61  VGNEVQPSSPTAA-FVVPAMRNIQTAISNAGLANQIKVSTAIDTGVLGESFPPSKGSFRN 120
           VGNEV  S P AA FV PAM+NIQ AIS AGL  QIKVSTAIDT V+G SFPPS G+F+ 
Sbjct: 116 VGNEVLSSDPGAARFVGPAMQNIQNAISAAGLGGQIKVSTAIDTRVIGVSFPPSAGAFKT 175

Query: 121 DALSLLNPIIRFLNENRSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQLRYQN 180
           D    ++P+IRFL  NRSPLL+NLYPYFSYIGN   IRLDYALFTAPSVVVTDGQ RYQN
Sbjct: 176 DVRGFMDPVIRFLVANRSPLLLNLYPYFSYIGNTRDIRLDYALFTAPSVVVTDGQFRYQN 235

Query: 181 LFDAILDAVYAALEKANGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPR 240
           LFDAILD+ YAALEKA GG+LEIVISESGWPSAGG ATSIDNARTYNNNLIQHVKRGTPR
Sbjct: 236 LFDAILDSFYAALEKAGGGNLEIVISESGWPSAGGTATSIDNARTYNNNLIQHVKRGTPR 295

Query: 241 RSGRPIETYIFAMFDENQKSPEFEKHFGLFSPNKQLKYPVNFN 283
           R GRPIETYIF MFDENQKSPEFEKHFGLFSPNKQ KYP+NFN
Sbjct: 296 RPGRPIETYIFGMFDENQKSPEFEKHFGLFSPNKQRKYPINFN 338

[Close]

BLAST of Lag0035265 vs. TAIR 10
Match: AT3G57240.1 (beta-1,3-glucanase 3 )

HSP 1 Score: 377.1 bits (967), Expect = 1.2e-104
Identity = 197/284 (69.37%), Postives = 228/284 (80.28%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNIELMLGLPNPDLQRIASSQAEANSWVQNNVRNYPNVRFKYIV 60
           MRLYDPN+ +L+ALRGSNIEL+L +PNPDLQR+ASSQAEA++WV+NNVRNY NV F+YI 
Sbjct: 64  MRLYDPNQETLNALRGSNIELVLDVPNPDLQRLASSQAEADTWVRNNVRNYANVTFRYIS 123

Query: 61  VGNEVQPSSPTAAFVVPAMRNIQTAISNAGLANQIKVSTAIDT-GVLGESFPPSKGSFRN 120
           VGNEVQPS   A+FV+PAM+NI+ A+S+ G    IKVSTAIDT G+ G  FPPS G+F  
Sbjct: 124 VGNEVQPSDQAASFVLPAMQNIERAVSSLG----IKVSTAIDTRGISG--FPPSSGTFTP 183

Query: 121 DALSLLNPIIRFLNENRSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQLRYQN 180
           +  S + P+I FL+  +SPLLVN YPYFSY GN   IRLDY LFTAPS VV DGQ +Y+N
Sbjct: 184 EFRSFIAPVISFLSSKQSPLLVNNYPYFSYTGNMRDIRLDYTLFTAPSTVVNDGQNQYRN 243

Query: 181 LFDAILDAVYAALEKANGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPR 240
           LF AILD VYA+LEKA GGSLEIV+SESGWP+AGGAAT +DNARTY NNLIQ VK G+PR
Sbjct: 244 LFHAILDTVYASLEKAGGGSLEIVVSESGWPTAGGAATGVDNARTYVNNLIQTVKNGSPR 303

Query: 241 RSGRPIETYIFAMFDENQK-SPEFEKHFGLFSPNKQLKYPVNFN 283
           R GR  ETYIFAMFDEN K  PE EK +GLF PN Q KY VNFN
Sbjct: 304 RPGRATETYIFAMFDENSKQGPETEKFWGLFLPNLQPKYVVNFN 341

[Close]

BLAST of Lag0035265 vs. TAIR 10
Match: AT3G57270.1 (beta-1,3-glucanase 1 )

HSP 1 Score: 354.0 bits (907), Expect = 1.1e-97
Identity = 180/284 (63.38%), Postives = 219/284 (77.11%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNIELMLGLPNPDLQRIASSQAEANSWVQNNVRNYPN-VRFKYI 60
           +RLY P+   L ALRGSNIE+ LGLPN  LQ +ASSQ++AN+WVQ  V NY N VRF+YI
Sbjct: 56  VRLYSPDHDVLAALRGSNIEVTLGLPNSYLQSVASSQSQANAWVQTYVMNYANGVRFRYI 115

Query: 61  VVGNEVQPSSPTAAFVVPAMRNIQTAISNAGLANQIKVSTAIDTGVLGESFPPSKGSFRN 120
            VGNEV+ S   A F+VPAM NI  A+  AGL  +IKVST++D GVL ES+PPSKGSFR 
Sbjct: 116 SVGNEVKISDSYAQFLVPAMENIDRAVLAAGLGGRIKVSTSVDMGVLRESYPPSKGSFRG 175

Query: 121 DALSLLNPIIRFLNENRSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQLRYQN 180
           D + ++ PIIRFL    SPLL+NLY YFSY GN   IRLDYALFTAPS +V+D    YQN
Sbjct: 176 DVMVVMEPIIRFLVSKNSPLLLNLYTYFSYAGNVGQIRLDYALFTAPSGIVSDPPRSYQN 235

Query: 181 LFDAILDAVYAALEKANGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPR 240
           LFDA+LDA+Y+ALEK+ G SLEIV++E+GWP+ GG  T+I+NAR YNNNLI+HVK GTP+
Sbjct: 236 LFDAMLDAMYSALEKSGGASLEIVVAETGWPTGGGTDTNIENARIYNNNLIKHVKNGTPK 295

Query: 241 RSGRPIETYIFAMFDENQK--SPEFEKHFGLFSPNKQLKYPVNF 282
           R G+ IETY+FA++DENQK   P  EK +GLF PNKQ KY +NF
Sbjct: 296 RPGKEIETYLFAIYDENQKPTPPYVEKFWGLFYPNKQPKYDINF 339

[Close]

BLAST of Lag0035265 vs. TAIR 10
Match: AT3G57260.1 (beta-1,3-glucanase 2 )

HSP 1 Score: 338.2 bits (866), Expect = 6.4e-93
Identity = 173/283 (61.13%), Postives = 218/283 (77.03%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNIELMLGLPNPDLQRIASSQAEANSWVQNNVRNY-PNVRFKYI 60
           MRLY P+  +L ALRGS+IEL+L +P+ DL+R+ASSQ EA+ WVQ NV++Y   VRF+YI
Sbjct: 61  MRLYGPDPGALAALRGSDIELILDVPSSDLERLASSQTEADKWVQENVQSYRDGVRFRYI 120

Query: 61  VVGNEVQPSSPTAAFVVPAMRNIQTAISNAGLANQIKVSTAIDTGVLGESFPPSKGSFRN 120
            VGNEV+PS     F++ AM+NI+ A+S AGL  ++KVSTAI T    ++ PPS+G FR+
Sbjct: 121 NVGNEVKPS--VGGFLLQAMQNIENAVSGAGL--EVKVSTAIATDTTTDTSPPSQGRFRD 180

Query: 121 DALSLLNPIIRFLNENRSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQLRYQN 180
           +  S L P+I FL   +SPLLVNLYPYFSY+G+ + I LDYALFTA S V  D    YQN
Sbjct: 181 EYKSFLEPVIGFLASKQSPLLVNLYPYFSYMGDTANIHLDYALFTAQSTVDNDPGYSYQN 240

Query: 181 LFDAILDAVYAALEKANGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPR 240
           LFDA LD+VYAALEK+ GGSLEIV+SE+GWP+ G   TS++NA+TY NNLIQHVK G+PR
Sbjct: 241 LFDANLDSVYAALEKSGGGSLEIVVSETGWPTEGAVGTSVENAKTYVNNLIQHVKNGSPR 300

Query: 241 RSGRPIETYIFAMFDENQKSPEFEKHFGLFSPNKQLKYPVNFN 283
           R G+ IETYIFAMFDEN+K P +EK +GLF P++Q KY VNFN
Sbjct: 301 RPGKAIETYIFAMFDENKKEPTYEKFWGLFHPDRQSKYEVNFN 339

[Close]

BLAST of Lag0035265 vs. TAIR 10
Match: AT4G16260.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 334.0 bits (855), Expect = 1.2e-91
Identity = 169/285 (59.30%), Postives = 212/285 (74.39%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNIELMLGLPNPDLQRIASSQAEANSWVQNNVRN-YPNVRFKYI 60
           +RLYDPN+A+L+ALR + IE+++G+PN DL R  ++ + A SW+QNNV N YP V FKYI
Sbjct: 53  VRLYDPNQAALNALRNTGIEVIIGVPNTDL-RSLTNPSSARSWLQNNVLNYYPAVSFKYI 112

Query: 61  VVGNEVQPSSPTAAFVVPAMRNIQTAISNAGLANQIKVSTAIDTGVLGESFPPSKGSFRN 120
            VGNEV PS+     V+PAMRN+  A+  A L ++IKVSTAID  ++G SFPPS G FR 
Sbjct: 113 AVGNEVSPSN-GGDVVLPAMRNVYDALRGANLQDRIKVSTAIDMTLIGNSFPPSSGEFRG 172

Query: 121 DALSLLNPIIRFLNENRSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQLRYQN 180
           D    ++P+I FL    S LL N+YPYFSY+ NP  I L YALFT+PSVVV DG   YQN
Sbjct: 173 DVRWYIDPVIGFLTSTNSALLANIYPYFSYVDNPRDISLSYALFTSPSVVVWDGSRGYQN 232

Query: 181 LFDAILDAVYAALEKANGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVK--RGT 240
           LFDA+LD VY+A+E++ GGSL +V+SESGWPS GG A S DNAR +  NL   V+  RGT
Sbjct: 233 LFDALLDVVYSAVERSGGGSLPVVVSESGWPSNGGNAASFDNARAFYTNLASRVRENRGT 292

Query: 241 PRRSGRPIETYIFAMFDENQKSPEFEKHFGLFSPNKQLKYPVNFN 283
           P+R GR +ETY+FAMFDENQKSPE EK+FGLF PNKQ K+P+ F+
Sbjct: 293 PKRPGRGVETYLFAMFDENQKSPEIEKNFGLFFPNKQPKFPITFS 335

[Close]

BLAST of Lag0035265 vs. TAIR 10
Match: AT5G56590.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 237.7 bits (605), Expect = 1.2e-62
Identity = 123/292 (42.12%), Postives = 192/292 (65.75%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNIELMLGLPNPDLQRIASSQAEANSWVQNNV-RNYPNVRFKYI 60
           +R+YD N   L A   ++IELM+G+PN DL   + SQ+  ++W++N+V   YP  +  YI
Sbjct: 55  VRIYDYNSQVLKAFGNTSIELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYI 114

Query: 61  VVGNEV--QPSSPTAAFVVPAMRNIQTAISNAGLANQIKVSTAIDTGVLGESFPPSKGSF 120
            VG E    P    ++FVVPAM+N+ TA+   GL+ +IKVST +  G+L  SFPPS G+F
Sbjct: 115 TVGAESTDDPHINASSFVVPAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAF 174

Query: 121 RNDALSLLNPIIRFLNENRSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQ-LR 180
            +     L P++ FL EN+SP +++LYPY++Y  +P+ + LDY LF + S V+     L 
Sbjct: 175 NSSYAYFLRPMLEFLAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLL 234

Query: 181 YQNLFDAILDAVYAALEKANGGSLEIVISESGWPSAGG----AATSIDNARTYNNNLIQH 240
           Y+N+FDA +DA+Y AL   N  +++I+++E+GWP+ G     AA S DNA TYN+N+I+H
Sbjct: 235 YKNMFDAQVDALYYALTALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRH 294

Query: 241 V--KRGTPRRSGRPIETYIFAMFDENQKSP-EFEKHFGLFSPNKQLKYPVNF 282
           V   +GTP + G  +  YIF++F+EN+K+  + E+++GLF P++   Y ++F
Sbjct: 295 VVTNQGTPAKPGEAMNVYIFSLFNENRKAGLDSERNWGLFYPDQTSVYQLDF 346

The following BLAST results are available for this feature:

BLAST of Lag0035265 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038876764.1	2.5e-131	84.75	glucan endo-1,3-beta-glucosidase, basic isoform-like [Benincasa hispida]	[more]
TYK19849.1	4.7e-130	82.62	glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo var. makuwa]	[more]
XP_022987104.1	1.8e-129	82.27	glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucurbita maxima]	[more]
KAG6590084.1	8.9e-129	82.27	Glucan endo-1,3-beta-glucosidase, basic isoform, partial [Cucurbita argyrosperma...	[more]
KAG7023754.1	2.0e-128	81.91	Glucan endo-1,3-beta-glucosidase, basic isoform, partial [Cucurbita argyrosperma...	[more]

BLAST of Lag0035265 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P52408	4.7e-117	74.82	Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS...	[more]
P07979	1.9e-105	66.32	Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3	[more]
Q03773	5.4e-105	66.67	Glucan endo-1,3-beta-glucosidase OS=Glycine max OX=3847 PE=1 SV=1	[more]
F4J270	1.7e-103	69.37	Probable glucan endo-1,3-beta-glucosidase BG3 OS=Arabidopsis thaliana OX=3702 GN...	[more]
P36401	3.9e-103	66.08	Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum OX=4...	[more]

BLAST of Lag0035265 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D8R0	2.3e-130	82.62	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A6J1JHX8	8.6e-130	82.27	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484757 PE=...	[more]
A0A5A7TA86	1.2e-128	81.98	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A1S3B9H8	1.2e-128	81.98	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487244 PE=3 SV...	[more]
A0A6J1CAZ7	2.4e-127	82.69	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111010014 ...	[more]

BLAST of Lag0035265 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G57240.1	1.2e-104	69.37	beta-1,3-glucanase 3	[more]
AT3G57270.1	1.1e-97	63.38	beta-1,3-glucanase 1	[more]
AT3G57260.1	6.4e-93	61.13	beta-1,3-glucanase 2	[more]
AT4G16260.1	1.2e-91	59.30	Glycosyl hydrolase superfamily protein	[more]
AT5G56590.1	1.2e-62	42.12	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 1..281 e-value: 5.9E-110 score: 367.7
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 200..213
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 1..282 e-value: 1.7E-124 score: 416.9
None	No IPR available	PANTHER	PTHR32227:SF138	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG3-RELATED	coord: 1..279
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 1..279
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 1..280

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0035265.1	Lag0035265.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds