Lag0035253 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0035253
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Glucan endo-1,3-beta-glucosidase
Location	chr3: 17491013 .. 17491861 (+)
RNA-Seq Expression	Lag0035253
Synteny	Lag0035253

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGACTCTATGATCCCAACAGAGCTTCCCTCGACGCTCTTAGAGGTTCCAACACTGAACTCATGCTTGGACTCCCCAACTCTGACCTCCAAAGAATCGCTTCAAGTCAGACCGAAGCGAATTCATGGGTTCAAAACAACGTTAGAAACTATCCTAACGTTAGATTCAGGTACGTCGCTGTCGGCAATGAAGTCCAACCCTCTAGTTCTGCTGCTTCATTCGTTGTCTCAGCAATGCGAAACATCCAAACTGCAATTTCCAATGCTGGTTTGGCTAATCAAATCAAAGTCTCCACCGCTATTGACACTGGTGTCCTCGGCGAGTCTTATCCTCCTTCTAAAGGCTCCTTTAAAACTGAGGCCTTATCGCTACTCAACCCCATCATTCGTTTTCTCAATGAAAATCACTCACCATTGCTCGTCAACTTGTACCCTTACTTTAGTTACATTGGTAACCCGAGTGCCATTCGTTTGGACTATGCCCTCTTCACGGCGCCATCAGTGGTGGTCACCGATGGCCAATATCGTTACCAAAATTTGTTCGATGCAATGGTCGATGCCGTTTACGCAGCATTGGAAAAGGCCAGTGGAGGAAGTTTGGAGATTGTGATATCAGAGAGTGGATGGCCATCGGCAGGGGGTGCAGCAACGTCGATTGATAACGCACGAACATACAACAACAATTTGATTCAACATGTCAAGAGAGGAACTCCCAGGCGTTCTGGAAAACCGATTCAGACTTATATTTTTGCCATGTTTGATGAAAATCAAAAGAGTCCTGAGTATGAGAAGCATTTTGGTCTCTTCTCTCCAAACAAGCAATCTAAATATCCGGTCAACTTCAATTGA

mRNA sequence

ATGAGACTCTATGATCCCAACAGAGCTTCCCTCGACGCTCTTAGAGGTTCCAACACTGAACTCATGCTTGGACTCCCCAACTCTGACCTCCAAAGAATCGCTTCAAGTCAGACCGAAGCGAATTCATGGGTTCAAAACAACGTTAGAAACTATCCTAACGTTAGATTCAGGTACGTCGCTGTCGGCAATGAAGTCCAACCCTCTAGTTCTGCTGCTTCATTCGTTGTCTCAGCAATGCGAAACATCCAAACTGCAATTTCCAATGCTGGTTTGGCTAATCAAATCAAAGTCTCCACCGCTATTGACACTGGTGTCCTCGGCGAGTCTTATCCTCCTTCTAAAGGCTCCTTTAAAACTGAGGCCTTATCGCTACTCAACCCCATCATTCGTTTTCTCAATGAAAATCACTCACCATTGCTCGTCAACTTGTACCCTTACTTTAGTTACATTGGTAACCCGAGTGCCATTCGTTTGGACTATGCCCTCTTCACGGCGCCATCAGTGGTGGTCACCGATGGCCAATATCGTTACCAAAATTTGTTCGATGCAATGGTCGATGCCGTTTACGCAGCATTGGAAAAGGCCAGTGGAGGAAGTTTGGAGATTGTGATATCAGAGAGTGGATGGCCATCGGCAGGGGGTGCAGCAACGTCGATTGATAACGCACGAACATACAACAACAATTTGATTCAACATGTCAAGAGAGGAACTCCCAGGCGTTCTGGAAAACCGATTCAGACTTATATTTTTGCCATGTTTGATGAAAATCAAAAGAGTCCTGAGTATGAGAAGCATTTTGGTCTCTTCTCTCCAAACAAGCAATCTAAATATCCGGTCAACTTCAATTGA

Coding sequence (CDS)

ATGAGACTCTATGATCCCAACAGAGCTTCCCTCGACGCTCTTAGAGGTTCCAACACTGAACTCATGCTTGGACTCCCCAACTCTGACCTCCAAAGAATCGCTTCAAGTCAGACCGAAGCGAATTCATGGGTTCAAAACAACGTTAGAAACTATCCTAACGTTAGATTCAGGTACGTCGCTGTCGGCAATGAAGTCCAACCCTCTAGTTCTGCTGCTTCATTCGTTGTCTCAGCAATGCGAAACATCCAAACTGCAATTTCCAATGCTGGTTTGGCTAATCAAATCAAAGTCTCCACCGCTATTGACACTGGTGTCCTCGGCGAGTCTTATCCTCCTTCTAAAGGCTCCTTTAAAACTGAGGCCTTATCGCTACTCAACCCCATCATTCGTTTTCTCAATGAAAATCACTCACCATTGCTCGTCAACTTGTACCCTTACTTTAGTTACATTGGTAACCCGAGTGCCATTCGTTTGGACTATGCCCTCTTCACGGCGCCATCAGTGGTGGTCACCGATGGCCAATATCGTTACCAAAATTTGTTCGATGCAATGGTCGATGCCGTTTACGCAGCATTGGAAAAGGCCAGTGGAGGAAGTTTGGAGATTGTGATATCAGAGAGTGGATGGCCATCGGCAGGGGGTGCAGCAACGTCGATTGATAACGCACGAACATACAACAACAATTTGATTCAACATGTCAAGAGAGGAACTCCCAGGCGTTCTGGAAAACCGATTCAGACTTATATTTTTGCCATGTTTGATGAAAATCAAAAGAGTCCTGAGTATGAGAAGCATTTTGGTCTCTTCTCTCCAAACAAGCAATCTAAATATCCGGTCAACTTCAATTGA

Protein sequence

MRLYDPNRASLDALRGSNTELMLGLPNSDLQRIASSQTEANSWVQNNVRNYPNVRFRYVAVGNEVQPSSSAASFVVSAMRNIQTAISNAGLANQIKVSTAIDTGVLGESYPPSKGSFKTEALSLLNPIIRFLNENHSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQYRYQNLFDAMVDAVYAALEKASGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPRRSGKPIQTYIFAMFDENQKSPEYEKHFGLFSPNKQSKYPVNFN

Homology

[Close]

BLAST of Lag0035253 vs. NCBI nr
Match: XP_038876764.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Benincasa hispida])

HSP 1 Score: 465.7 bits (1197), Expect = 2.8e-127
Identity = 232/282 (82.27%), Postives = 254/282 (90.07%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNTELMLGLPNSDLQRIASSQTEANSWVQNNVRNYPNVRFRYVA 60
           MRLYDPNRASLDALRGSN ELMLGLPNSDLQRIASSQ+EAN+WVQNNV+++ NVRF+Y+A
Sbjct: 56  MRLYDPNRASLDALRGSNIELMLGLPNSDLQRIASSQSEANAWVQNNVKSFGNVRFKYIA 115

Query: 61  VGNEVQPSSSAASFVVSAMRNIQTAISNAGLANQIKVSTAIDTGVLGESYPPSKGSFKTE 120
           VGNEVQPSSSAA FVV AM+NIQTA+SN GLANQIKVSTAIDTG+LG S+PPS G+F  +
Sbjct: 116 VGNEVQPSSSAAQFVVPAMQNIQTALSNGGLANQIKVSTAIDTGLLGVSFPPSSGAFNNQ 175

Query: 121 ALSLLNPIIRFLNENHSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQYRYQNL 180
           A+ LLNPIIRFLN N SPLLVNLYP+FSYI NP  IRLDYALFTA S VVTDGQ RYQNL
Sbjct: 176 AMPLLNPIIRFLNANRSPLLVNLYPFFSYISNPRDIRLDYALFTAKSTVVTDGQLRYQNL 235

Query: 181 FDAMVDAVYAALEKASGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPRR 240
           FDA+VDA+YAALEKA G SLEIV+SESGWPSAGG A S +NARTYNNNLIQHVKRGTPRR
Sbjct: 236 FDAIVDAIYAALEKAGGSSLEIVVSESGWPSAGGTAASTENARTYNNNLIQHVKRGTPRR 295

Query: 241 SGKPIQTYIFAMFDENQKSPEYEKHFGLFSPNKQSKYPVNFN 283
             +PI+TYIFAMFDENQKSPE+EKHFGLF PNKQ KY VNFN
Sbjct: 296 PQRPIETYIFAMFDENQKSPEFEKHFGLFFPNKQPKYAVNFN 337

[Close]

BLAST of Lag0035253 vs. NCBI nr
Match: TYK19849.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo var. makuwa])

HSP 1 Score: 464.2 bits (1193), Expect = 8.3e-127
Identity = 229/282 (81.21%), Postives = 252/282 (89.36%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNTELMLGLPNSDLQRIASSQTEANSWVQNNVRNYPNVRFRYVA 60
           MRLYDPNR SLDALRGS  ELMLGLPN DLQRIASSQ EAN+WVQNN++NY NVRF+Y+A
Sbjct: 23  MRLYDPNRGSLDALRGSTIELMLGLPNPDLQRIASSQAEANAWVQNNIKNYQNVRFKYIA 82

Query: 61  VGNEVQPSSSAASFVVSAMRNIQTAISNAGLANQIKVSTAIDTGVLGESYPPSKGSFKTE 120
           VGNEVQPSSSAASFVV AMRNIQTA++NAGLAN+IKVSTAIDT +L  SYPPS G+ K E
Sbjct: 83  VGNEVQPSSSAASFVVPAMRNIQTALNNAGLANRIKVSTAIDTSILAMSYPPSSGTIKNE 142

Query: 121 ALSLLNPIIRFLNENHSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQYRYQNL 180
            +  ++ +IRFL +N SPLLVNLYPYFSYIGNP  IRLDYALFTAPS VV DGQ+RYQNL
Sbjct: 143 DMPFMSSVIRFLKDNGSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQFRYQNL 202

Query: 181 FDAMVDAVYAALEKASGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPRR 240
           FDA++DAVYAALEK  GG+LEIVISESGWPSAGG A SIDNARTY NNLIQHVKRGTPRR
Sbjct: 203 FDAILDAVYAALEKVGGGNLEIVISESGWPSAGGTAASIDNARTYINNLIQHVKRGTPRR 262

Query: 241 SGKPIQTYIFAMFDENQKSPEYEKHFGLFSPNKQSKYPVNFN 283
            G+PI+TYIFAMFDENQK+PE+EKHFGLF PNKQSKYPVNFN
Sbjct: 263 PGRPIETYIFAMFDENQKNPEFEKHFGLFFPNKQSKYPVNFN 304

[Close]

BLAST of Lag0035253 vs. NCBI nr
Match: XP_031737288.1 (glucan endo-1,3-beta-glucosidase, basic isoform [Cucumis sativus] >KGN66700.1 hypothetical protein Csa_007701 [Cucumis sativus])

HSP 1 Score: 461.5 bits (1186), Expect = 5.4e-126
Identity = 229/282 (81.21%), Postives = 253/282 (89.72%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNTELMLGLPNSDLQRIASSQTEANSWVQNNVRNYPNVRFRYVA 60
           MRLYDPNR SLDALRGS  ELMLG+PNSDLQRIASSQTEAN+WVQNNV+NYPNVRFRY+A
Sbjct: 56  MRLYDPNRGSLDALRGSPIELMLGVPNSDLQRIASSQTEANAWVQNNVKNYPNVRFRYIA 115

Query: 61  VGNEVQPSSSAASFVVSAMRNIQTAISNAGLANQIKVSTAIDTGVLGESYPPSKGSFKTE 120
           VGNEVQPSSSAASFVV AM NIQTA++NAGL  +IKVSTA+ T ++ +SYPPS+G+ K E
Sbjct: 116 VGNEVQPSSSAASFVVPAMVNIQTALNNAGL-GKIKVSTAVATSIMADSYPPSRGTIKNE 175

Query: 121 ALSLLNPIIRFLNENHSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQYRYQNL 180
            + L+NPIIRFLN N SPLL+NLYPYFSYIGNP  IRLDYALFTAPS VV DGQY YQNL
Sbjct: 176 VMPLMNPIIRFLNNNRSPLLLNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQYLYQNL 235

Query: 181 FDAMVDAVYAALEKASGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPRR 240
           FDAM+DA+YAALEK  GG+LEIVISESGWPSAGG ATSI+NARTY NNLIQHVKRGTPRR
Sbjct: 236 FDAMLDALYAALEKVGGGNLEIVISESGWPSAGGTATSINNARTYINNLIQHVKRGTPRR 295

Query: 241 SGKPIQTYIFAMFDENQKSPEYEKHFGLFSPNKQSKYPVNFN 283
            G+P +TYIFAMFDEN+KSPE EKHFGLF PNKQSKYP+NFN
Sbjct: 296 PGRPTETYIFAMFDENKKSPELEKHFGLFFPNKQSKYPINFN 336

[Close]

BLAST of Lag0035253 vs. NCBI nr
Match: KAA0038295.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo var. makuwa])

HSP 1 Score: 458.4 bits (1178), Expect = 4.5e-125
Identity = 228/283 (80.57%), Postives = 251/283 (88.69%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNTELMLGLPNSDLQRIASSQTEANSWVQNNVRNYPNVRFRYVA 60
           MRLYDPNR SLDALRGS  ELMLGLPN DLQRIASSQ EAN+WVQNN++NY NVRF+Y+A
Sbjct: 23  MRLYDPNRGSLDALRGSTIELMLGLPNPDLQRIASSQAEANAWVQNNIKNYQNVRFKYIA 82

Query: 61  VGNEVQPSSSAASFVVSAMRNIQTAISNAGLANQIKVSTAIDTGVLGESYPPSKGSFKTE 120
           VGNEVQPSSSAASFVV AMRNIQTA++NAGL N+IKVSTAIDT +L  SYPPS G+ K E
Sbjct: 83  VGNEVQPSSSAASFVVPAMRNIQTALNNAGLVNRIKVSTAIDTSILAMSYPPSSGTIKNE 142

Query: 121 ALSLLNPIIRFLNENHSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQYRYQNL 180
            +  ++ +IRFL +N SPLLVNLYPYFSYIGNP  IRLDYALFTAPS VV DGQ+RYQNL
Sbjct: 143 DMPFMSSVIRFLKDNGSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQFRYQNL 202

Query: 181 FDAMVDAVYAALEKASGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPRR 240
           FDA++DAVYAALEK  GG+LEIVISESGWPSAGG A SIDNARTY NNLIQHVKRGTPRR
Sbjct: 203 FDAILDAVYAALEKVGGGNLEIVISESGWPSAGGTAASIDNARTYINNLIQHVKRGTPRR 262

Query: 241 -SGKPIQTYIFAMFDENQKSPEYEKHFGLFSPNKQSKYPVNFN 283
             G+PI+TYIFAMFDENQK+PE+EKHFGLF PNKQSKYPVNFN
Sbjct: 263 PGGRPIETYIFAMFDENQKNPEFEKHFGLFFPNKQSKYPVNFN 305

[Close]

BLAST of Lag0035253 vs. NCBI nr
Match: XP_008443726.1 (PREDICTED: glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo])

HSP 1 Score: 458.4 bits (1178), Expect = 4.5e-125
Identity = 228/283 (80.57%), Postives = 251/283 (88.69%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNTELMLGLPNSDLQRIASSQTEANSWVQNNVRNYPNVRFRYVA 60
           MRLYDPNR SLDALRGS  ELMLGLPN DLQRIASSQ EAN+WVQNN++NY NVRF+Y+A
Sbjct: 57  MRLYDPNRGSLDALRGSTIELMLGLPNPDLQRIASSQAEANAWVQNNIKNYQNVRFKYIA 116

Query: 61  VGNEVQPSSSAASFVVSAMRNIQTAISNAGLANQIKVSTAIDTGVLGESYPPSKGSFKTE 120
           VGNEVQPSSSAASFVV AMRNIQTA++NAGL N+IKVSTAIDT +L  SYPPS G+ K E
Sbjct: 117 VGNEVQPSSSAASFVVPAMRNIQTALNNAGLVNRIKVSTAIDTSILAMSYPPSSGTIKNE 176

Query: 121 ALSLLNPIIRFLNENHSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQYRYQNL 180
            +  ++ +IRFL +N SPLLVNLYPYFSYIGNP  IRLDYALFTAPS VV DGQ+RYQNL
Sbjct: 177 DMPFMSSVIRFLKDNGSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQFRYQNL 236

Query: 181 FDAMVDAVYAALEKASGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPRR 240
           FDA++DAVYAALEK  GG+LEIVISESGWPSAGG A SIDNARTY NNLIQHVKRGTPRR
Sbjct: 237 FDAILDAVYAALEKVGGGNLEIVISESGWPSAGGTAASIDNARTYINNLIQHVKRGTPRR 296

Query: 241 -SGKPIQTYIFAMFDENQKSPEYEKHFGLFSPNKQSKYPVNFN 283
             G+PI+TYIFAMFDENQK+PE+EKHFGLF PNKQSKYPVNFN
Sbjct: 297 PGGRPIETYIFAMFDENQKNPEFEKHFGLFFPNKQSKYPVNFN 339

[Close]

BLAST of Lag0035253 vs. ExPASy Swiss-Prot
Match: P52408 (Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS1 PE=3 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 1.7e-114
Identity = 207/282 (73.40%), Postives = 242/282 (85.82%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNTELMLGLPNSDLQRIASSQTEANSWVQNNVRNYPNVRFRYVA 60
           MRLYDPN A+L+ALRGSN +L+LG+PN +LQ IA SQ  AN+WVQNNVRNY NV+F+Y+A
Sbjct: 69  MRLYDPNPAALEALRGSNIKLLLGVPNENLQYIALSQANANAWVQNNVRNYANVKFKYIA 128

Query: 61  VGNEVQPSSSAASFVVSAMRNIQTAISNAGLANQIKVSTAIDTGVLGESYPPSKGSFKTE 120
           VGNEV+PS S A F+V AMRNIQ AIS AGLA +IKVSTAIDTGVLGE++PPS GSFK+E
Sbjct: 129 VGNEVKPSDSFAQFLVPAMRNIQEAISLAGLAKKIKVSTAIDTGVLGETFPPSIGSFKSE 188

Query: 121 ALSLLNPIIRFLNENHSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQYRYQNL 180
             +LL PIIRFL  + SPLLVNLYPYF+Y GN   IRLDYALFTAPSVVV DG + Y+NL
Sbjct: 189 YNALLYPIIRFLVSHQSPLLVNLYPYFAYSGNTQDIRLDYALFTAPSVVVQDGNFGYRNL 248

Query: 181 FDAMVDAVYAALEKASGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPRR 240
           FDAM+D VYAALEKA GGSL++VISE+GWPSA G AT+IDNART+ +NLIQHVK GTPRR
Sbjct: 249 FDAMLDGVYAALEKAGGGSLKVVISETGWPSAAGTATTIDNARTFISNLIQHVKEGTPRR 308

Query: 241 SGKPIQTYIFAMFDENQKSPEYEKHFGLFSPNKQSKYPVNFN 283
            G+PI+TYIFAMFDEN+K+PE EKH+GLFSP KQ KY ++FN
Sbjct: 309 PGRPIETYIFAMFDENRKTPELEKHWGLFSPTKQPKYQISFN 350

[Close]

BLAST of Lag0035253 vs. ExPASy Swiss-Prot
Match: Q03773 (Glucan endo-1,3-beta-glucosidase OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 8.7e-103
Identity = 188/285 (65.96%), Postives = 231/285 (81.05%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNTELMLGLPNSDLQRIASSQTEANSWVQNNVRNYP-NVRFRYV 60
           MR+Y P+   L+ALRGSN EL+L +PN +L+ +ASSQ  AN WVQ+N++NY  NVRFRYV
Sbjct: 63  MRIYGPSPEVLEALRGSNIELLLDIPNDNLRNLASSQDNANKWVQDNIKNYANNVRFRYV 122

Query: 61  AVGNEVQPSSSAASFVVSAMRNIQTAISNAGLANQIKVSTAIDTGVLGESYPPSKGSFKT 120
           +VGNEV+P  S A F+V A+ NIQ AISNAGL NQ+KVSTAIDTG L ES+PPSKGSFK+
Sbjct: 123 SVGNEVKPEHSFAQFLVPALENIQRAISNAGLGNQVKVSTAIDTGALAESFPPSKGSFKS 182

Query: 121 EAL-SLLNPIIRFLNENHSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQYRYQ 180
           +   + L+ +IRFL  N++PL+VN+Y YF+Y  NP  I LDYALF +PSVVV DG   Y+
Sbjct: 183 DYRGAYLDGVIRFLVNNNAPLMVNVYSYFAYTANPKDISLDYALFRSPSVVVQDGSLGYR 242

Query: 181 NLFDAMVDAVYAALEKASGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTP 240
           NLFDA VDAVYAALEKA GGSL IV+SESGWPS+GG ATS+DNARTYN NL+++VK+GTP
Sbjct: 243 NLFDASVDAVYAALEKAGGGSLNIVVSESGWPSSGGTATSLDNARTYNTNLVRNVKQGTP 302

Query: 241 RRSGKPIQTYIFAMFDENQKSPEYEKHFGLFSP-NKQSKYPVNFN 283
           +R G P++TY+FAMFDENQK PE+EK +GLFSP  KQ KY +NFN
Sbjct: 303 KRPGAPLETYVFAMFDENQKQPEFEKFWGLFSPITKQPKYSINFN 347

[Close]

BLAST of Lag0035253 vs. ExPASy Swiss-Prot
Match: P07979 (Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3)

HSP 1 Score: 373.6 bits (958), Expect = 1.9e-102
Identity = 186/285 (65.26%), Postives = 230/285 (80.70%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNTELMLGLPNSDLQRIASSQTEANSWVQNNVRNY-PNVRFRYV 60
           MRLYDPN+A+L ALRGSN E+MLG+PNSDLQ IA++ + AN+WVQ NVRN+ P V+FRY+
Sbjct: 62  MRLYDPNQAALQALRGSNIEVMLGVPNSDLQNIAANPSNANNWVQRNVRNFWPAVKFRYI 121

Query: 61  AVGNEVQP---SSSAASFVVSAMRNIQTAISNAGLANQIKVSTAIDTGVLGESYPPSKGS 120
           AVGNEV P   +SS   +++ AMRNI+ AIS+AGL N IKVS+++D  ++G S+PPS+GS
Sbjct: 122 AVGNEVSPVTGTSSLTRYLLPAMRNIRNAISSAGLQNNIKVSSSVDMTLIGNSFPPSQGS 181

Query: 121 FKTEALSLLNPIIRFLNENHSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQYR 180
           F+ +  S ++PII F+   +SPLLVN+YPYFSY GNP  I L YALFTAP+VVV DG   
Sbjct: 182 FRNDVRSFIDPIIGFVRRINSPLLVNIYPYFSYAGNPRDISLPYALFTAPNVVVQDGSLG 241

Query: 181 YQNLFDAMVDAVYAALEKASGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRG 240
           Y+NLFDAM DAVYAAL +A GGS+EIV+SESGWPSAG  A + +NA TY  NLIQHVKRG
Sbjct: 242 YRNLFDAMSDAVYAALSRAGGGSIEIVVSESGWPSAGAFAATTNNAATYYKNLIQHVKRG 301

Query: 241 TPRRSGKPIQTYIFAMFDENQKSPEYEKHFGLFSPNKQSKYPVNF 282
           +PRR  K I+TY+FAMFDEN K+PE EKHFGLFSPNKQ KYP++F
Sbjct: 302 SPRRPNKVIETYLFAMFDENNKNPELEKHFGLFSPNKQPKYPLSF 346

[Close]

BLAST of Lag0035253 vs. ExPASy Swiss-Prot
Match: F4J270 (Probable glucan endo-1,3-beta-glucosidase BG3 OS=Arabidopsis thaliana OX=3702 GN=BG3 PE=2 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 1.5e-99
Identity = 191/284 (67.25%), Postives = 225/284 (79.23%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNTELMLGLPNSDLQRIASSQTEANSWVQNNVRNYPNVRFRYVA 60
           MRLYDPN+ +L+ALRGSN EL+L +PN DLQR+ASSQ EA++WV+NNVRNY NV FRY++
Sbjct: 64  MRLYDPNQETLNALRGSNIELVLDVPNPDLQRLASSQAEADTWVRNNVRNYANVTFRYIS 123

Query: 61  VGNEVQPSSSAASFVVSAMRNIQTAISNAGLANQIKVSTAIDT-GVLGESYPPSKGSFKT 120
           VGNEVQPS  AASFV+ AM+NI+ A+S+ G    IKVSTAIDT G+ G  +PPS G+F  
Sbjct: 124 VGNEVQPSDQAASFVLPAMQNIERAVSSLG----IKVSTAIDTRGISG--FPPSSGTFTP 183

Query: 121 EALSLLNPIIRFLNENHSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQYRYQN 180
           E  S + P+I FL+   SPLLVN YPYFSY GN   IRLDY LFTAPS VV DGQ +Y+N
Sbjct: 184 EFRSFIAPVISFLSSKQSPLLVNNYPYFSYTGNMRDIRLDYTLFTAPSTVVNDGQNQYRN 243

Query: 181 LFDAMVDAVYAALEKASGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPR 240
           LF A++D VYA+LEKA GGSLEIV+SESGWP+AGGAAT +DNARTY NNLIQ VK G+PR
Sbjct: 244 LFHAILDTVYASLEKAGGGSLEIVVSESGWPTAGGAATGVDNARTYVNNLIQTVKNGSPR 303

Query: 241 RSGKPIQTYIFAMFDENQK-SPEYEKHFGLFSPNKQSKYPVNFN 283
           R G+  +TYIFAMFDEN K  PE EK +GLF PN Q KY VNFN
Sbjct: 304 RPGRATETYIFAMFDENSKQGPETEKFWGLFLPNLQPKYVVNFN 341

[Close]

BLAST of Lag0035253 vs. ExPASy Swiss-Prot
Match: P36401 (Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum OX=4097 PE=1 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 3.4e-99
Identity = 184/286 (64.34%), Postives = 227/286 (79.37%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNTELMLGLPNSDLQRIASSQTEANSWVQNNVRNYPNVRFRYVA 60
           MR+YDP++ +L+ALRGSN ELMLG+PN DL+ +A+SQ  A++WVQNNVRNY NV+FRY+A
Sbjct: 55  MRIYDPDQPTLEALRGSNIELMLGVPNPDLENVAASQANADTWVQNNVRNYGNVKFRYIA 114

Query: 61  VGNEVQPSSSAASFV---VSAMRNIQTAISNAGLANQIKVSTAIDTGVLGESYPPSKGSF 120
           VGNEV P +  + +V   ++AMRNIQTAIS AGL NQIKVSTAI+TG+  ++ PPS G F
Sbjct: 115 VGNEVSPLNENSKYVPVLLNAMRNIQTAISGAGLGNQIKVSTAIETGLTTDTSPPSNGRF 174

Query: 121 KTEALSLLNPIIRFLNENHSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQYRY 180
           K +    + PII FL  N +PLLVNLYPYF+ I N + I+L+YALFT+  VVV D    Y
Sbjct: 175 KDDVRQFIEPIINFLVTNRAPLLVNLYPYFA-IANNADIKLEYALFTSSEVVVNDNGRGY 234

Query: 181 QNLFDAMVDAVYAALEKASGGSLEIVISESGWPSAG-GAATSIDNARTYNNNLIQHVKRG 240
           +NLFDA++DA Y+ALEKASG SLEIV+SESGWPSAG G  TSIDNARTYNNNLI HVK G
Sbjct: 235 RNLFDAILDATYSALEKASGSSLEIVVSESGWPSAGAGQLTSIDNARTYNNNLISHVKGG 294

Query: 241 TPRRSGKPIQTYIFAMFDENQKSPEYEKHFGLFSPNKQSKYPVNFN 283
           +P+R   PI+TY+FA+FDE+QK PE EKHFGLFS N Q KY ++FN
Sbjct: 295 SPKRPSGPIETYVFALFDEDQKDPEIEKHFGLFSANMQPKYQISFN 339

[Close]

BLAST of Lag0035253 vs. ExPASy TrEMBL
Match: A0A5D3D8R0 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold811G00600 PE=3 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 4.0e-127
Identity = 229/282 (81.21%), Postives = 252/282 (89.36%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNTELMLGLPNSDLQRIASSQTEANSWVQNNVRNYPNVRFRYVA 60
           MRLYDPNR SLDALRGS  ELMLGLPN DLQRIASSQ EAN+WVQNN++NY NVRF+Y+A
Sbjct: 23  MRLYDPNRGSLDALRGSTIELMLGLPNPDLQRIASSQAEANAWVQNNIKNYQNVRFKYIA 82

Query: 61  VGNEVQPSSSAASFVVSAMRNIQTAISNAGLANQIKVSTAIDTGVLGESYPPSKGSFKTE 120
           VGNEVQPSSSAASFVV AMRNIQTA++NAGLAN+IKVSTAIDT +L  SYPPS G+ K E
Sbjct: 83  VGNEVQPSSSAASFVVPAMRNIQTALNNAGLANRIKVSTAIDTSILAMSYPPSSGTIKNE 142

Query: 121 ALSLLNPIIRFLNENHSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQYRYQNL 180
            +  ++ +IRFL +N SPLLVNLYPYFSYIGNP  IRLDYALFTAPS VV DGQ+RYQNL
Sbjct: 143 DMPFMSSVIRFLKDNGSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQFRYQNL 202

Query: 181 FDAMVDAVYAALEKASGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPRR 240
           FDA++DAVYAALEK  GG+LEIVISESGWPSAGG A SIDNARTY NNLIQHVKRGTPRR
Sbjct: 203 FDAILDAVYAALEKVGGGNLEIVISESGWPSAGGTAASIDNARTYINNLIQHVKRGTPRR 262

Query: 241 SGKPIQTYIFAMFDENQKSPEYEKHFGLFSPNKQSKYPVNFN 283
            G+PI+TYIFAMFDENQK+PE+EKHFGLF PNKQSKYPVNFN
Sbjct: 263 PGRPIETYIFAMFDENQKNPEFEKHFGLFFPNKQSKYPVNFN 304

[Close]

BLAST of Lag0035253 vs. ExPASy TrEMBL
Match: A0A0A0LXW2 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G660200 PE=3 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 2.6e-126
Identity = 229/282 (81.21%), Postives = 253/282 (89.72%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNTELMLGLPNSDLQRIASSQTEANSWVQNNVRNYPNVRFRYVA 60
           MRLYDPNR SLDALRGS  ELMLG+PNSDLQRIASSQTEAN+WVQNNV+NYPNVRFRY+A
Sbjct: 56  MRLYDPNRGSLDALRGSPIELMLGVPNSDLQRIASSQTEANAWVQNNVKNYPNVRFRYIA 115

Query: 61  VGNEVQPSSSAASFVVSAMRNIQTAISNAGLANQIKVSTAIDTGVLGESYPPSKGSFKTE 120
           VGNEVQPSSSAASFVV AM NIQTA++NAGL  +IKVSTA+ T ++ +SYPPS+G+ K E
Sbjct: 116 VGNEVQPSSSAASFVVPAMVNIQTALNNAGL-GKIKVSTAVATSIMADSYPPSRGTIKNE 175

Query: 121 ALSLLNPIIRFLNENHSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQYRYQNL 180
            + L+NPIIRFLN N SPLL+NLYPYFSYIGNP  IRLDYALFTAPS VV DGQY YQNL
Sbjct: 176 VMPLMNPIIRFLNNNRSPLLLNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQYLYQNL 235

Query: 181 FDAMVDAVYAALEKASGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPRR 240
           FDAM+DA+YAALEK  GG+LEIVISESGWPSAGG ATSI+NARTY NNLIQHVKRGTPRR
Sbjct: 236 FDAMLDALYAALEKVGGGNLEIVISESGWPSAGGTATSINNARTYINNLIQHVKRGTPRR 295

Query: 241 SGKPIQTYIFAMFDENQKSPEYEKHFGLFSPNKQSKYPVNFN 283
            G+P +TYIFAMFDEN+KSPE EKHFGLF PNKQSKYP+NFN
Sbjct: 296 PGRPTETYIFAMFDENKKSPELEKHFGLFFPNKQSKYPINFN 336

[Close]

BLAST of Lag0035253 vs. ExPASy TrEMBL
Match: A0A5A7TA86 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold270G001600 PE=3 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 2.2e-125
Identity = 228/283 (80.57%), Postives = 251/283 (88.69%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNTELMLGLPNSDLQRIASSQTEANSWVQNNVRNYPNVRFRYVA 60
           MRLYDPNR SLDALRGS  ELMLGLPN DLQRIASSQ EAN+WVQNN++NY NVRF+Y+A
Sbjct: 23  MRLYDPNRGSLDALRGSTIELMLGLPNPDLQRIASSQAEANAWVQNNIKNYQNVRFKYIA 82

Query: 61  VGNEVQPSSSAASFVVSAMRNIQTAISNAGLANQIKVSTAIDTGVLGESYPPSKGSFKTE 120
           VGNEVQPSSSAASFVV AMRNIQTA++NAGL N+IKVSTAIDT +L  SYPPS G+ K E
Sbjct: 83  VGNEVQPSSSAASFVVPAMRNIQTALNNAGLVNRIKVSTAIDTSILAMSYPPSSGTIKNE 142

Query: 121 ALSLLNPIIRFLNENHSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQYRYQNL 180
            +  ++ +IRFL +N SPLLVNLYPYFSYIGNP  IRLDYALFTAPS VV DGQ+RYQNL
Sbjct: 143 DMPFMSSVIRFLKDNGSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQFRYQNL 202

Query: 181 FDAMVDAVYAALEKASGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPRR 240
           FDA++DAVYAALEK  GG+LEIVISESGWPSAGG A SIDNARTY NNLIQHVKRGTPRR
Sbjct: 203 FDAILDAVYAALEKVGGGNLEIVISESGWPSAGGTAASIDNARTYINNLIQHVKRGTPRR 262

Query: 241 -SGKPIQTYIFAMFDENQKSPEYEKHFGLFSPNKQSKYPVNFN 283
             G+PI+TYIFAMFDENQK+PE+EKHFGLF PNKQSKYPVNFN
Sbjct: 263 PGGRPIETYIFAMFDENQKNPEFEKHFGLFFPNKQSKYPVNFN 305

[Close]

BLAST of Lag0035253 vs. ExPASy TrEMBL
Match: A0A1S3B9H8 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487244 PE=3 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 2.2e-125
Identity = 228/283 (80.57%), Postives = 251/283 (88.69%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNTELMLGLPNSDLQRIASSQTEANSWVQNNVRNYPNVRFRYVA 60
           MRLYDPNR SLDALRGS  ELMLGLPN DLQRIASSQ EAN+WVQNN++NY NVRF+Y+A
Sbjct: 57  MRLYDPNRGSLDALRGSTIELMLGLPNPDLQRIASSQAEANAWVQNNIKNYQNVRFKYIA 116

Query: 61  VGNEVQPSSSAASFVVSAMRNIQTAISNAGLANQIKVSTAIDTGVLGESYPPSKGSFKTE 120
           VGNEVQPSSSAASFVV AMRNIQTA++NAGL N+IKVSTAIDT +L  SYPPS G+ K E
Sbjct: 117 VGNEVQPSSSAASFVVPAMRNIQTALNNAGLVNRIKVSTAIDTSILAMSYPPSSGTIKNE 176

Query: 121 ALSLLNPIIRFLNENHSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQYRYQNL 180
            +  ++ +IRFL +N SPLLVNLYPYFSYIGNP  IRLDYALFTAPS VV DGQ+RYQNL
Sbjct: 177 DMPFMSSVIRFLKDNGSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQFRYQNL 236

Query: 181 FDAMVDAVYAALEKASGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPRR 240
           FDA++DAVYAALEK  GG+LEIVISESGWPSAGG A SIDNARTY NNLIQHVKRGTPRR
Sbjct: 237 FDAILDAVYAALEKVGGGNLEIVISESGWPSAGGTAASIDNARTYINNLIQHVKRGTPRR 296

Query: 241 -SGKPIQTYIFAMFDENQKSPEYEKHFGLFSPNKQSKYPVNFN 283
             G+PI+TYIFAMFDENQK+PE+EKHFGLF PNKQSKYPVNFN
Sbjct: 297 PGGRPIETYIFAMFDENQKNPEFEKHFGLFFPNKQSKYPVNFN 339

[Close]

BLAST of Lag0035253 vs. ExPASy TrEMBL
Match: A0A6J1JHX8 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484757 PE=3 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 5.4e-124
Identity = 222/282 (78.72%), Postives = 254/282 (90.07%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNTELMLGLPNSDLQRIASSQTEANSWVQNNVRNYPNVRFRYVA 60
           MRLY PNR SL AL GSN EL+L +PN DLQRIASSQ EAN+WVQNN+R++PNVRF+Y++
Sbjct: 56  MRLYGPNRDSLGALSGSNIELILDVPNPDLQRIASSQAEANTWVQNNIRSFPNVRFKYIS 115

Query: 61  VGNEVQPSSSAASFVVSAMRNIQTAISNAGLANQIKVSTAIDTGVLGESYPPSKGSFKTE 120
           VGNEVQPSSS A FVV AMRNIQTA++N GL+++IKVST+IDTGV+G S+PPS G+FK+E
Sbjct: 116 VGNEVQPSSSNAQFVVPAMRNIQTALANVGLSSRIKVSTSIDTGVIGTSFPPSNGAFKSE 175

Query: 121 ALSLLNPIIRFLNENHSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQYRYQNL 180
           A+ LLNPIIRFLNEN SPLLVNLYPYFSYIGN   IRLDYALFTAPS VVTDGQ+RYQNL
Sbjct: 176 AMPLLNPIIRFLNENSSPLLVNLYPYFSYIGNMRDIRLDYALFTAPSTVVTDGQFRYQNL 235

Query: 181 FDAMVDAVYAALEKASGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPRR 240
           FDA++DA YAALEKA G +LEIV+SESGWP+AGGAATSI NARTY NNLIQHVKRGTPRR
Sbjct: 236 FDAILDATYAALEKAGGRNLEIVVSESGWPTAGGAATSISNARTYINNLIQHVKRGTPRR 295

Query: 241 SGKPIQTYIFAMFDENQKSPEYEKHFGLFSPNKQSKYPVNFN 283
            G+PI+ YIFAMFDENQK+PE+EKHFGLF+PNKQ KYP+NFN
Sbjct: 296 PGRPIEAYIFAMFDENQKNPEFEKHFGLFTPNKQLKYPINFN 337

[Close]

BLAST of Lag0035253 vs. TAIR 10
Match: AT3G57240.1 (beta-1,3-glucanase 3 )

HSP 1 Score: 364.0 bits (933), Expect = 1.1e-100
Identity = 191/284 (67.25%), Postives = 225/284 (79.23%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNTELMLGLPNSDLQRIASSQTEANSWVQNNVRNYPNVRFRYVA 60
           MRLYDPN+ +L+ALRGSN EL+L +PN DLQR+ASSQ EA++WV+NNVRNY NV FRY++
Sbjct: 64  MRLYDPNQETLNALRGSNIELVLDVPNPDLQRLASSQAEADTWVRNNVRNYANVTFRYIS 123

Query: 61  VGNEVQPSSSAASFVVSAMRNIQTAISNAGLANQIKVSTAIDT-GVLGESYPPSKGSFKT 120
           VGNEVQPS  AASFV+ AM+NI+ A+S+ G    IKVSTAIDT G+ G  +PPS G+F  
Sbjct: 124 VGNEVQPSDQAASFVLPAMQNIERAVSSLG----IKVSTAIDTRGISG--FPPSSGTFTP 183

Query: 121 EALSLLNPIIRFLNENHSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQYRYQN 180
           E  S + P+I FL+   SPLLVN YPYFSY GN   IRLDY LFTAPS VV DGQ +Y+N
Sbjct: 184 EFRSFIAPVISFLSSKQSPLLVNNYPYFSYTGNMRDIRLDYTLFTAPSTVVNDGQNQYRN 243

Query: 181 LFDAMVDAVYAALEKASGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPR 240
           LF A++D VYA+LEKA GGSLEIV+SESGWP+AGGAAT +DNARTY NNLIQ VK G+PR
Sbjct: 244 LFHAILDTVYASLEKAGGGSLEIVVSESGWPTAGGAATGVDNARTYVNNLIQTVKNGSPR 303

Query: 241 RSGKPIQTYIFAMFDENQK-SPEYEKHFGLFSPNKQSKYPVNFN 283
           R G+  +TYIFAMFDEN K  PE EK +GLF PN Q KY VNFN
Sbjct: 304 RPGRATETYIFAMFDENSKQGPETEKFWGLFLPNLQPKYVVNFN 341

[Close]

BLAST of Lag0035253 vs. TAIR 10
Match: AT3G57270.1 (beta-1,3-glucanase 1 )

HSP 1 Score: 350.1 bits (897), Expect = 1.6e-96
Identity = 179/284 (63.03%), Postives = 221/284 (77.82%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNTELMLGLPNSDLQRIASSQTEANSWVQNNVRNYPN-VRFRYV 60
           +RLY P+   L ALRGSN E+ LGLPNS LQ +ASSQ++AN+WVQ  V NY N VRFRY+
Sbjct: 56  VRLYSPDHDVLAALRGSNIEVTLGLPNSYLQSVASSQSQANAWVQTYVMNYANGVRFRYI 115

Query: 61  AVGNEVQPSSSAASFVVSAMRNIQTAISNAGLANQIKVSTAIDTGVLGESYPPSKGSFKT 120
           +VGNEV+ S S A F+V AM NI  A+  AGL  +IKVST++D GVL ESYPPSKGSF+ 
Sbjct: 116 SVGNEVKISDSYAQFLVPAMENIDRAVLAAGLGGRIKVSTSVDMGVLRESYPPSKGSFRG 175

Query: 121 EALSLLNPIIRFLNENHSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQYRYQN 180
           + + ++ PIIRFL   +SPLL+NLY YFSY GN   IRLDYALFTAPS +V+D    YQN
Sbjct: 176 DVMVVMEPIIRFLVSKNSPLLLNLYTYFSYAGNVGQIRLDYALFTAPSGIVSDPPRSYQN 235

Query: 181 LFDAMVDAVYAALEKASGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPR 240
           LFDAM+DA+Y+ALEK+ G SLEIV++E+GWP+ GG  T+I+NAR YNNNLI+HVK GTP+
Sbjct: 236 LFDAMLDAMYSALEKSGGASLEIVVAETGWPTGGGTDTNIENARIYNNNLIKHVKNGTPK 295

Query: 241 RSGKPIQTYIFAMFDENQK--SPEYEKHFGLFSPNKQSKYPVNF 282
           R GK I+TY+FA++DENQK   P  EK +GLF PNKQ KY +NF
Sbjct: 296 RPGKEIETYLFAIYDENQKPTPPYVEKFWGLFYPNKQPKYDINF 339

[Close]

BLAST of Lag0035253 vs. TAIR 10
Match: AT3G57260.1 (beta-1,3-glucanase 2 )

HSP 1 Score: 341.7 bits (875), Expect = 5.8e-94
Identity = 176/283 (62.19%), Postives = 219/283 (77.39%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNTELMLGLPNSDLQRIASSQTEANSWVQNNVRNY-PNVRFRYV 60
           MRLY P+  +L ALRGS+ EL+L +P+SDL+R+ASSQTEA+ WVQ NV++Y   VRFRY+
Sbjct: 61  MRLYGPDPGALAALRGSDIELILDVPSSDLERLASSQTEADKWVQENVQSYRDGVRFRYI 120

Query: 61  AVGNEVQPSSSAASFVVSAMRNIQTAISNAGLANQIKVSTAIDTGVLGESYPPSKGSFKT 120
            VGNEV+P  S   F++ AM+NI+ A+S AGL  ++KVSTAI T    ++ PPS+G F+ 
Sbjct: 121 NVGNEVKP--SVGGFLLQAMQNIENAVSGAGL--EVKVSTAIATDTTTDTSPPSQGRFRD 180

Query: 121 EALSLLNPIIRFLNENHSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQYRYQN 180
           E  S L P+I FL    SPLLVNLYPYFSY+G+ + I LDYALFTA S V  D  Y YQN
Sbjct: 181 EYKSFLEPVIGFLASKQSPLLVNLYPYFSYMGDTANIHLDYALFTAQSTVDNDPGYSYQN 240

Query: 181 LFDAMVDAVYAALEKASGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVKRGTPR 240
           LFDA +D+VYAALEK+ GGSLEIV+SE+GWP+ G   TS++NA+TY NNLIQHVK G+PR
Sbjct: 241 LFDANLDSVYAALEKSGGGSLEIVVSETGWPTEGAVGTSVENAKTYVNNLIQHVKNGSPR 300

Query: 241 RSGKPIQTYIFAMFDENQKSPEYEKHFGLFSPNKQSKYPVNFN 283
           R GK I+TYIFAMFDEN+K P YEK +GLF P++QSKY VNFN
Sbjct: 301 RPGKAIETYIFAMFDENKKEPTYEKFWGLFHPDRQSKYEVNFN 339

[Close]

BLAST of Lag0035253 vs. TAIR 10
Match: AT4G16260.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 319.3 bits (817), Expect = 3.1e-87
Identity = 160/285 (56.14%), Postives = 213/285 (74.74%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNTELMLGLPNSDLQRIASSQTEANSWVQNNVRN-YPNVRFRYV 60
           +RLYDPN+A+L+ALR +  E+++G+PN+DL R  ++ + A SW+QNNV N YP V F+Y+
Sbjct: 53  VRLYDPNQAALNALRNTGIEVIIGVPNTDL-RSLTNPSSARSWLQNNVLNYYPAVSFKYI 112

Query: 61  AVGNEVQPSSSAASFVVSAMRNIQTAISNAGLANQIKVSTAIDTGVLGESYPPSKGSFKT 120
           AVGNEV P S+    V+ AMRN+  A+  A L ++IKVSTAID  ++G S+PPS G F+ 
Sbjct: 113 AVGNEVSP-SNGGDVVLPAMRNVYDALRGANLQDRIKVSTAIDMTLIGNSFPPSSGEFRG 172

Query: 121 EALSLLNPIIRFLNENHSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQYRYQN 180
           +    ++P+I FL   +S LL N+YPYFSY+ NP  I L YALFT+PSVVV DG   YQN
Sbjct: 173 DVRWYIDPVIGFLTSTNSALLANIYPYFSYVDNPRDISLSYALFTSPSVVVWDGSRGYQN 232

Query: 181 LFDAMVDAVYAALEKASGGSLEIVISESGWPSAGGAATSIDNARTYNNNLIQHVK--RGT 240
           LFDA++D VY+A+E++ GGSL +V+SESGWPS GG A S DNAR +  NL   V+  RGT
Sbjct: 233 LFDALLDVVYSAVERSGGGSLPVVVSESGWPSNGGNAASFDNARAFYTNLASRVRENRGT 292

Query: 241 PRRSGKPIQTYIFAMFDENQKSPEYEKHFGLFSPNKQSKYPVNFN 283
           P+R G+ ++TY+FAMFDENQKSPE EK+FGLF PNKQ K+P+ F+
Sbjct: 293 PKRPGRGVETYLFAMFDENQKSPEIEKNFGLFFPNKQPKFPITFS 335

[Close]

BLAST of Lag0035253 vs. TAIR 10
Match: AT5G56590.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 232.3 bits (591), Expect = 4.9e-61
Identity = 122/292 (41.78%), Postives = 193/292 (66.10%), Query Frame = 0


Query: 1   MRLYDPNRASLDALRGSNTELMLGLPNSDLQRIASSQTEANSWVQNNV-RNYPNVRFRYV 60
           +R+YD N   L A   ++ ELM+G+PNSDL   + SQ+  ++W++N+V   YP  +  Y+
Sbjct: 55  VRIYDYNSQVLKAFGNTSIELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYI 114

Query: 61  AVGNEV--QPSSSAASFVVSAMRNIQTAISNAGLANQIKVSTAIDTGVLGESYPPSKGSF 120
            VG E    P  +A+SFVV AM+N+ TA+   GL+ +IKVST +  G+L  S+PPS G+F
Sbjct: 115 TVGAESTDDPHINASSFVVPAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAF 174

Query: 121 KTEALSLLNPIIRFLNENHSPLLVNLYPYFSYIGNPSAIRLDYALFTAPSVVVTDGQ-YR 180
            +     L P++ FL EN SP +++LYPY++Y  +P+ + LDY LF + S V+       
Sbjct: 175 NSSYAYFLRPMLEFLAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLL 234

Query: 181 YQNLFDAMVDAVYAALEKASGGSLEIVISESGWPSAGG----AATSIDNARTYNNNLIQH 240
           Y+N+FDA VDA+Y AL   +  +++I+++E+GWP+ G     AA S DNA TYN+N+I+H
Sbjct: 235 YKNMFDAQVDALYYALTALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRH 294

Query: 241 V--KRGTPRRSGKPIQTYIFAMFDENQKSP-EYEKHFGLFSPNKQSKYPVNF 282
           V   +GTP + G+ +  YIF++F+EN+K+  + E+++GLF P++ S Y ++F
Sbjct: 295 VVTNQGTPAKPGEAMNVYIFSLFNENRKAGLDSERNWGLFYPDQTSVYQLDF 346

The following BLAST results are available for this feature:

BLAST of Lag0035253 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038876764.1	2.8e-127	82.27	glucan endo-1,3-beta-glucosidase, basic isoform-like [Benincasa hispida]	[more]
TYK19849.1	8.3e-127	81.21	glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo var. makuwa]	[more]
XP_031737288.1	5.4e-126	81.21	glucan endo-1,3-beta-glucosidase, basic isoform [Cucumis sativus] >KGN66700.1 hy...	[more]
KAA0038295.1	4.5e-125	80.57	glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo var. makuwa]	[more]
XP_008443726.1	4.5e-125	80.57	PREDICTED: glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo]	[more]

BLAST of Lag0035253 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P52408	1.7e-114	73.40	Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS...	[more]
Q03773	8.7e-103	65.96	Glucan endo-1,3-beta-glucosidase OS=Glycine max OX=3847 PE=1 SV=1	[more]
P07979	1.9e-102	65.26	Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3	[more]
F4J270	1.5e-99	67.25	Probable glucan endo-1,3-beta-glucosidase BG3 OS=Arabidopsis thaliana OX=3702 GN...	[more]
P36401	3.4e-99	64.34	Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum OX=4...	[more]

BLAST of Lag0035253 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D8R0	4.0e-127	81.21	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A0A0LXW2	2.6e-126	81.21	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G660200 PE=3...	[more]
A0A5A7TA86	2.2e-125	80.57	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A1S3B9H8	2.2e-125	80.57	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487244 PE=3 SV...	[more]
A0A6J1JHX8	5.4e-124	78.72	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484757 PE=...	[more]

BLAST of Lag0035253 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G57240.1	1.1e-100	67.25	beta-1,3-glucanase 3	[more]
AT3G57270.1	1.6e-96	63.03	beta-1,3-glucanase 1	[more]
AT3G57260.1	5.8e-94	62.19	beta-1,3-glucanase 2	[more]
AT4G16260.1	3.1e-87	56.14	Glycosyl hydrolase superfamily protein	[more]
AT5G56590.1	4.9e-61	41.78	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 1..282 e-value: 4.4E-123 score: 412.2
None	No IPR available	PANTHER	PTHR32227:SF138	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG3-RELATED	coord: 1..279
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 1..281 e-value: 1.1E-108 score: 363.6
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 200..213
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 1..279
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 1..280

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0035253.1	Lag0035253.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds