Lag0035176 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0035176
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionProtein TIC 20
Locationchr3: 16190353 .. 16190979 (+)
RNA-Seq ExpressionLag0035176
SyntenyLag0035176
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCACTTCCATCCCTCTCATCAACCTCTCCACCAAATCCGCCATTAAAGCCTCTCTAATCCCTCACTTCTCCGCCCTCACATCTCGCCACCGTTCCTCTCTCCCTCTTCTCCCCTCAAAACCCGCAAAATGCCCCGCCATTCGCATGTCCTACAACCCTACGCCGGCCACCGACCGCCTCATCTCCGCCGTTGCCTACACTCTCCCCTTCTTCAACTCCCTCCAGTACGGCCGCTTCCTCTTCGCCCAGTACCCGGCCCTCGGCCTCGCCTTCCAGCCTCTCCTCCCGATTCTAAGCCTTTATCGCTCCATTCCACACTCGAGCTTCGTTGCCTTCTTCGCGCTCTACTTGGGCGTGGTCCGGAACCCTAGCTTCAGCCACTACGTGAGGTTCAATTCGATGCAGGCGGTGACTCTGGACGTGCTGCTGGTGGTGCCGTTGTTGATACAGAGGATTCTGAGCCCCGGTCGGGGCGGATTAGGGTTCCAGATCATGGTTTGGGGTCACAATGCATTGTTTGTTTTCAGCGTTTTGTGCTTTGTTTACAGTCTGGTTTCTTGCCTTCTGGGTCGAACCCCTCACTTGCCCCTTGTTGCTGATGCTGCTGGCAGACAGATTTAG

mRNA sequence

ATGGCTTCCACTTCCATCCCTCTCATCAACCTCTCCACCAAATCCGCCATTAAAGCCTCTCTAATCCCTCACTTCTCCGCCCTCACATCTCGCCACCGTTCCTCTCTCCCTCTTCTCCCCTCAAAACCCGCAAAATGCCCCGCCATTCGCATGTCCTACAACCCTACGCCGGCCACCGACCGCCTCATCTCCGCCGTTGCCTACACTCTCCCCTTCTTCAACTCCCTCCAGTACGGCCGCTTCCTCTTCGCCCAGTACCCGGCCCTCGGCCTCGCCTTCCAGCCTCTCCTCCCGATTCTAAGCCTTTATCGCTCCATTCCACACTCGAGCTTCGTTGCCTTCTTCGCGCTCTACTTGGGCGTGGTCCGGAACCCTAGCTTCAGCCACTACGTGAGGTTCAATTCGATGCAGGCGGTGACTCTGGACGTGCTGCTGGTGGTGCCGTTGTTGATACAGAGGATTCTGAGCCCCGGTCGGGGCGGATTAGGGTTCCAGATCATGGTTTGGGGTCACAATGCATTGTTTGTTTTCAGCGTTTTGTGCTTTGTTTACAGTCTGGTTTCTTGCCTTCTGGGTCGAACCCCTCACTTGCCCCTTGTTGCTGATGCTGCTGGCAGACAGATTTAG

Coding sequence (CDS)

ATGGCTTCCACTTCCATCCCTCTCATCAACCTCTCCACCAAATCCGCCATTAAAGCCTCTCTAATCCCTCACTTCTCCGCCCTCACATCTCGCCACCGTTCCTCTCTCCCTCTTCTCCCCTCAAAACCCGCAAAATGCCCCGCCATTCGCATGTCCTACAACCCTACGCCGGCCACCGACCGCCTCATCTCCGCCGTTGCCTACACTCTCCCCTTCTTCAACTCCCTCCAGTACGGCCGCTTCCTCTTCGCCCAGTACCCGGCCCTCGGCCTCGCCTTCCAGCCTCTCCTCCCGATTCTAAGCCTTTATCGCTCCATTCCACACTCGAGCTTCGTTGCCTTCTTCGCGCTCTACTTGGGCGTGGTCCGGAACCCTAGCTTCAGCCACTACGTGAGGTTCAATTCGATGCAGGCGGTGACTCTGGACGTGCTGCTGGTGGTGCCGTTGTTGATACAGAGGATTCTGAGCCCCGGTCGGGGCGGATTAGGGTTCCAGATCATGGTTTGGGGTCACAATGCATTGTTTGTTTTCAGCGTTTTGTGCTTTGTTTACAGTCTGGTTTCTTGCCTTCTGGGTCGAACCCCTCACTTGCCCCTTGTTGCTGATGCTGCTGGCAGACAGATTTAG

Protein sequence

MASTSIPLINLSTKSAIKASLIPHFSALTSRHRSSLPLLPSKPAKCPAIRMSYNPTPATDRLISAVAYTLPFFNSLQYGRFLFAQYPALGLAFQPLLPILSLYRSIPHSSFVAFFALYLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGLGFQIMVWGHNALFVFSVLCFVYSLVSCLLGRTPHLPLVADAAGRQI
Homology
[Close]
BLAST of Lag0035176 vs. NCBI nr
Match: XP_038879337.1 (protein TIC 20-II, chloroplastic [Benincasa hispida])

HSP 1 Score: 366.3 bits (939), Expect = 1.7e-97
Identity = 185/208 (88.94%), Postives = 196/208 (94.23%), Query Frame = 0 

Query: 1   MASTSIPLINLSTKSAIKASLIPHFSALTSRHRSSLPLLPSKPAKCPAIRMSYNPTPATD 60
           MAS+SIPL+NLS K+A +A+LIPH SA+ SRHRSSLP LPSKPAK    RMSYNPTPATD
Sbjct: 1   MASSSIPLLNLSVKTAFRAALIPHSSAIKSRHRSSLPFLPSKPAKSTVFRMSYNPTPATD 60

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPALGLAFQPLLPILSLYRSIPHSSFVAFFALYLG 120
           R+ISAVAYTLPFFNSLQYGRFLFAQYPALG AF+PLLPIL LYRSIPH+SFVAFFALYLG
Sbjct: 61  RVISAVAYTLPFFNSLQYGRFLFAQYPALGFAFEPLLPILGLYRSIPHASFVAFFALYLG 120

Query: 121 VVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGLGFQIMVWGHNALFVFSVL 180
           +VRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGLGFQIMVWGHN LFVFSVL
Sbjct: 121 LVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGLGFQIMVWGHNGLFVFSVL 180

Query: 181 CFVYSLVSCLLGRTPHLPLVADAAGRQI 209
           CFVYSLVSCLLGRTPHLP+VADAAGRQI
Sbjct: 181 CFVYSLVSCLLGRTPHLPIVADAAGRQI 208

[Close]
BLAST of Lag0035176 vs. NCBI nr
Match: XP_022987499.1 (protein TIC 20-II, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 358.2 bits (918), Expect = 4.7e-95
Identity = 179/208 (86.06%), Postives = 193/208 (92.79%), Query Frame = 0 

Query: 1   MASTSIPLINLSTKSAIKASLIPHFSALTSRHRSSLPLLPSKPAKCPAIRMSYNPTPATD 60
           MAS SIPL+NLSTK AIKA+LIPH+SA+ SRH  SLPLLPS+PAK PAIRMSYNPTPATD
Sbjct: 3   MASCSIPLLNLSTKPAIKAALIPHYSAIKSRHCFSLPLLPSRPAKSPAIRMSYNPTPATD 62

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPALGLAFQPLLPILSLYRSIPHSSFVAFFALYLG 120
           RLISAVAYTLPFFNSLQYGRFLFAQYP LGLAF+PLLPIL LYRSIPHSSF+AFFA YLG
Sbjct: 63  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG 122

Query: 121 VVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGLGFQIMVWGHNALFVFSVL 180
           VVRNPSFS Y RFNSMQA+TLDVLLVVPLL+QRILSPGR GLGFQIM W HN LFVFSVL
Sbjct: 123 VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGRSGLGFQIMAWSHNGLFVFSVL 182

Query: 181 CFVYSLVSCLLGRTPHLPLVADAAGRQI 209
           CF+YS+VSC+LGRTPHLP+VADAAGRQ+
Sbjct: 183 CFLYSVVSCILGRTPHLPIVADAAGRQV 210

[Close]
BLAST of Lag0035176 vs. NCBI nr
Match: XP_023005745.1 (protein TIC 20-II, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 356.3 bits (913), Expect = 1.8e-94
Identity = 181/208 (87.02%), Postives = 191/208 (91.83%), Query Frame = 0 

Query: 1   MASTSIPLINLSTKSAIKASLIPHFSALTSRHRSSLPLLPSKPAKCPAIRMSYNPTPATD 60
           MAST IPL+NL T +AIKA+LIPH S L SRHRSSLPLL SKPAK  A+RMSYNPTPATD
Sbjct: 1   MASTFIPLLNLPTHTAIKATLIPHLSVLKSRHRSSLPLLSSKPAKSVAVRMSYNPTPATD 60

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPALGLAFQPLLPILSLYRSIPHSSFVAFFALYLG 120
           RLISAVAYTLPFFNSLQYGRFLF QYP LGLAF+P+LPIL LYRSIPHSSF+AFFALYLG
Sbjct: 61  RLISAVAYTLPFFNSLQYGRFLFTQYPVLGLAFEPILPILGLYRSIPHSSFIAFFALYLG 120

Query: 121 VVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGLGFQIMVWGHNALFVFSVL 180
           VVRNP+F+ YVRFNSMQAVTLDVLL VPLLIQRILSPG GGLGFQIMVWGHN LF+FSVL
Sbjct: 121 VVRNPNFNRYVRFNSMQAVTLDVLLAVPLLIQRILSPGSGGLGFQIMVWGHNGLFLFSVL 180

Query: 181 CFVYSLVSCLLGRTPHLPLVADAAGRQI 209
           CFVYSL SCLLGRTPHLPLVADAAGRQI
Sbjct: 181 CFVYSLASCLLGRTPHLPLVADAAGRQI 208

[Close]
BLAST of Lag0035176 vs. NCBI nr
Match: XP_023005732.1 (protein TIC 20-II, chloroplastic-like isoform X1 [Cucurbita maxima] >XP_023005740.1 protein TIC 20-II, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 356.3 bits (913), Expect = 1.8e-94
Identity = 181/208 (87.02%), Postives = 191/208 (91.83%), Query Frame = 0 

Query: 1   MASTSIPLINLSTKSAIKASLIPHFSALTSRHRSSLPLLPSKPAKCPAIRMSYNPTPATD 60
           MAST IPL+NL T +AIKA+LIPH S L SRHRSSLPLL SKPAK  A+RMSYNPTPATD
Sbjct: 8   MASTFIPLLNLPTHTAIKATLIPHLSVLKSRHRSSLPLLSSKPAKSVAVRMSYNPTPATD 67

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPALGLAFQPLLPILSLYRSIPHSSFVAFFALYLG 120
           RLISAVAYTLPFFNSLQYGRFLF QYP LGLAF+P+LPIL LYRSIPHSSF+AFFALYLG
Sbjct: 68  RLISAVAYTLPFFNSLQYGRFLFTQYPVLGLAFEPILPILGLYRSIPHSSFIAFFALYLG 127

Query: 121 VVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGLGFQIMVWGHNALFVFSVL 180
           VVRNP+F+ YVRFNSMQAVTLDVLL VPLLIQRILSPG GGLGFQIMVWGHN LF+FSVL
Sbjct: 128 VVRNPNFNRYVRFNSMQAVTLDVLLAVPLLIQRILSPGSGGLGFQIMVWGHNGLFLFSVL 187

Query: 181 CFVYSLVSCLLGRTPHLPLVADAAGRQI 209
           CFVYSL SCLLGRTPHLPLVADAAGRQI
Sbjct: 188 CFVYSLASCLLGRTPHLPLVADAAGRQI 215

[Close]
BLAST of Lag0035176 vs. NCBI nr
Match: XP_023516082.1 (protein TIC 20-II, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 355.9 bits (912), Expect = 2.3e-94
Identity = 178/208 (85.58%), Postives = 192/208 (92.31%), Query Frame = 0 

Query: 1   MASTSIPLINLSTKSAIKASLIPHFSALTSRHRSSLPLLPSKPAKCPAIRMSYNPTPATD 60
           MAS SIPL+NLSTK AIKA+LIPH+SA+ SRH  SLPLLPSKPAK PAIRMSYNPTPATD
Sbjct: 1   MASCSIPLLNLSTKPAIKAALIPHYSAIKSRHCFSLPLLPSKPAKSPAIRMSYNPTPATD 60

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPALGLAFQPLLPILSLYRSIPHSSFVAFFALYLG 120
           RLISAVAYTLPFFNSLQYGRFLFAQYP LGLAF+PLLPIL LYRSIPHSSF+AFFA YLG
Sbjct: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG 120

Query: 121 VVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGLGFQIMVWGHNALFVFSVL 180
           VVRNPSFS Y RFNSMQA+TLDVLLVVPLL+QRILSPG+ GLGF IM W HN LFVFSVL
Sbjct: 121 VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGFHIMAWSHNGLFVFSVL 180

Query: 181 CFVYSLVSCLLGRTPHLPLVADAAGRQI 209
           CF+YS+VSC+LGRTPHLP+VADAAGRQ+
Sbjct: 181 CFLYSVVSCILGRTPHLPIVADAAGRQV 208

[Close]
BLAST of Lag0035176 vs. ExPASy Swiss-Prot
Match: O82251 (Protein TIC 20-II, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-II PE=2 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 9.3e-62
Identity = 116/169 (68.64%), Postives = 139/169 (82.25%), Query Frame = 0 

Query: 41  SKPAKCPAIRMSYNPTPATDRLISAVAYTLPFFNSLQYGRFLFAQYPALGLAFQPLLPIL 100
           S+ A     R SY PTPAT+R+IS  +Y LPFFNSLQYGRFLFAQYP LGL F+P+ PIL
Sbjct: 40  SRRATALTTRASYTPTPATERVISIASYALPFFNSLQYGRFLFAQYPRLGLLFEPIFPIL 99

Query: 101 SLYRSIPHSSFVAFFALYLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGR- 160
           +LYRS+P++SFVAFF LYLGVVRN SFS YVRFN+MQAVTLDVLL VP+L+ RIL PG+ 
Sbjct: 100 NLYRSVPYASFVAFFGLYLGVVRNTSFSRYVRFNAMQAVTLDVLLAVPVLLTRILDPGQG 159

Query: 161 GGLGFQIMVWGHNALFVFSVLCFVYSLVSCLLGRTPHLPLVADAAGRQI 209
           GG G + M+WGH  +FVFS +CFVY +VS LLG+TP++P VADAAGRQ+
Sbjct: 160 GGFGMKAMMWGHTGVFVFSFMCFVYGVVSSLLGKTPYIPFVADAAGRQL 208

[Close]
BLAST of Lag0035176 vs. ExPASy Swiss-Prot
Match: Q9FM67 (Protein TIC 20-v, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-V PE=2 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.3e-34
Identity = 71/151 (47.02%), Postives = 102/151 (67.55%), Query Frame = 0 

Query: 58  ATDRLISAVAYTLPFFNSLQYGRFLFAQYPALGLAFQPLLPILSLYRSIPHSSFVAFFAL 117
           A+DR+ISAV Y  PFF+ +QYG+F+  QY    +  QPL P +  ++S P + F+ F  L
Sbjct: 59  ASDRIISAVCYFYPFFDGIQYGKFIITQYQPFQILIQPLFPAIRAFKSFPFNGFLIFITL 118

Query: 118 YLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGLGFQIMVWGHNALFVF 177
           Y  VVRNP+FS YVRFN+MQA+ LDVLL+ P L++R  +P R G G  +++   + +F+F
Sbjct: 119 YFVVVRNPNFSRYVRFNTMQAIVLDVLLIFPDLLERSFNP-RDGFGLDVVMSLDSTVFLF 178

Query: 178 SVLCFVYSLVSCLLGRTPHLPLVADAAGRQI 209
            ++  +Y   +CL G TP LPLVA+AA RQ+
Sbjct: 179 LLVSLIYGFSACLFGLTPRLPLVAEAADRQV 208

[Close]
BLAST of Lag0035176 vs. ExPASy Swiss-Prot
Match: P73387 (Tic20 family protein OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=sll1737 PE=3 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.2e-21
Identity = 52/151 (34.44%), Postives = 92/151 (60.93%), Query Frame = 0 

Query: 60  DRLISAVAYTLPFFNSLQYGRFLFAQYPALGLAFQPLLPILSLYRSIPHSSFVAFFALYL 119
           DR  SA+ Y +P  ++  +G FL  Q+P L + + P++P+L  Y   P +SF+ F  L++
Sbjct: 11  DRFFSALIYVIPLIDAFMFGGFLLQQFPVLQIIYLPIMPLLQFYYQFPFASFIIFIVLFM 70

Query: 120 GVVRNPSFSHYVRFNSMQAVTLDVLL-VVPLLIQRILSPGRG-GLGFQIMVWGHNALFVF 179
            VVRN + SH++RFN+MQA+ + +LL +  L++  ++ P  G GL  + +   +N  F+ 
Sbjct: 71  AVVRNNNISHFIRFNAMQAILIGILLSLFGLIVAYVIQPVFGQGLVTETL---YNFAFLG 130

Query: 180 SVLCFVYSLVSCLLGRTPHLPLVADAAGRQI 209
           ++ C  + +V  +LGR   +P ++DAA  Q+
Sbjct: 131 ALACGFFGIVQSVLGRYAEIPTISDAAYSQV 158

[Close]
BLAST of Lag0035176 vs. ExPASy Swiss-Prot
Match: Q9ZST8 (Protein TIC 20, chloroplastic OS=Pisum sativum OX=3888 GN=TIC20 PE=1 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 2.5e-06
Identity = 42/156 (26.92%), Postives = 74/156 (47.44%), Query Frame = 0 

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPALGLAFQPL-LPILSLYRSIPHSSFVAFFAL-Y 120
           R +S + Y LPF  +  Y R  +  +P +   FQP+  P L    ++P  S +A+F + Y
Sbjct: 104 RTLSCIPYLLPFHQAWMYARTAYHLHPFIPY-FQPMTYPFLMAIGTLPRWSLIAYFLIAY 163

Query: 121 LGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGLGFQIMVWGHNALFVFS 180
           L +VR   + H+ RF+    + +++ L V  ++ R +         +   WG   +  ++
Sbjct: 164 LTIVRRKEWPHFFRFHVAVGMLIEIALQVTGIVSRWMP--------RSFYWGKLGMHFWT 223

Query: 181 VLCFV--YSLVSC----LLGRTPHLPLVADAAGRQI 209
              FV  ++ + C    L+G    +P V DAA  QI
Sbjct: 224 TAFFVFLFTTIECIRCALVGMYADVPFVCDAAYIQI 250

[Close]
BLAST of Lag0035176 vs. ExPASy TrEMBL
Match: A0A6J1JJ22 (Protein TIC 20 OS=Cucurbita maxima OX=3661 GN=LOC111485044 PE=3 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 2.3e-95
Identity = 179/208 (86.06%), Postives = 193/208 (92.79%), Query Frame = 0 

Query: 1   MASTSIPLINLSTKSAIKASLIPHFSALTSRHRSSLPLLPSKPAKCPAIRMSYNPTPATD 60
           MAS SIPL+NLSTK AIKA+LIPH+SA+ SRH  SLPLLPS+PAK PAIRMSYNPTPATD
Sbjct: 3   MASCSIPLLNLSTKPAIKAALIPHYSAIKSRHCFSLPLLPSRPAKSPAIRMSYNPTPATD 62

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPALGLAFQPLLPILSLYRSIPHSSFVAFFALYLG 120
           RLISAVAYTLPFFNSLQYGRFLFAQYP LGLAF+PLLPIL LYRSIPHSSF+AFFA YLG
Sbjct: 63  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG 122

Query: 121 VVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGLGFQIMVWGHNALFVFSVL 180
           VVRNPSFS Y RFNSMQA+TLDVLLVVPLL+QRILSPGR GLGFQIM W HN LFVFSVL
Sbjct: 123 VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGRSGLGFQIMAWSHNGLFVFSVL 182

Query: 181 CFVYSLVSCLLGRTPHLPLVADAAGRQI 209
           CF+YS+VSC+LGRTPHLP+VADAAGRQ+
Sbjct: 183 CFLYSVVSCILGRTPHLPIVADAAGRQV 210

[Close]
BLAST of Lag0035176 vs. ExPASy TrEMBL
Match: A0A6J1KY86 (Protein TIC 20 OS=Cucurbita maxima OX=3661 GN=LOC111498634 PE=3 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 8.7e-95
Identity = 181/208 (87.02%), Postives = 191/208 (91.83%), Query Frame = 0 

Query: 1   MASTSIPLINLSTKSAIKASLIPHFSALTSRHRSSLPLLPSKPAKCPAIRMSYNPTPATD 60
           MAST IPL+NL T +AIKA+LIPH S L SRHRSSLPLL SKPAK  A+RMSYNPTPATD
Sbjct: 8   MASTFIPLLNLPTHTAIKATLIPHLSVLKSRHRSSLPLLSSKPAKSVAVRMSYNPTPATD 67

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPALGLAFQPLLPILSLYRSIPHSSFVAFFALYLG 120
           RLISAVAYTLPFFNSLQYGRFLF QYP LGLAF+P+LPIL LYRSIPHSSF+AFFALYLG
Sbjct: 68  RLISAVAYTLPFFNSLQYGRFLFTQYPVLGLAFEPILPILGLYRSIPHSSFIAFFALYLG 127

Query: 121 VVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGLGFQIMVWGHNALFVFSVL 180
           VVRNP+F+ YVRFNSMQAVTLDVLL VPLLIQRILSPG GGLGFQIMVWGHN LF+FSVL
Sbjct: 128 VVRNPNFNRYVRFNSMQAVTLDVLLAVPLLIQRILSPGSGGLGFQIMVWGHNGLFLFSVL 187

Query: 181 CFVYSLVSCLLGRTPHLPLVADAAGRQI 209
           CFVYSL SCLLGRTPHLPLVADAAGRQI
Sbjct: 188 CFVYSLASCLLGRTPHLPLVADAAGRQI 215

[Close]
BLAST of Lag0035176 vs. ExPASy TrEMBL
Match: A0A6J1KY91 (Protein TIC 20 OS=Cucurbita maxima OX=3661 GN=LOC111498634 PE=3 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 8.7e-95
Identity = 181/208 (87.02%), Postives = 191/208 (91.83%), Query Frame = 0 

Query: 1   MASTSIPLINLSTKSAIKASLIPHFSALTSRHRSSLPLLPSKPAKCPAIRMSYNPTPATD 60
           MAST IPL+NL T +AIKA+LIPH S L SRHRSSLPLL SKPAK  A+RMSYNPTPATD
Sbjct: 1   MASTFIPLLNLPTHTAIKATLIPHLSVLKSRHRSSLPLLSSKPAKSVAVRMSYNPTPATD 60

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPALGLAFQPLLPILSLYRSIPHSSFVAFFALYLG 120
           RLISAVAYTLPFFNSLQYGRFLF QYP LGLAF+P+LPIL LYRSIPHSSF+AFFALYLG
Sbjct: 61  RLISAVAYTLPFFNSLQYGRFLFTQYPVLGLAFEPILPILGLYRSIPHSSFIAFFALYLG 120

Query: 121 VVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGLGFQIMVWGHNALFVFSVL 180
           VVRNP+F+ YVRFNSMQAVTLDVLL VPLLIQRILSPG GGLGFQIMVWGHN LF+FSVL
Sbjct: 121 VVRNPNFNRYVRFNSMQAVTLDVLLAVPLLIQRILSPGSGGLGFQIMVWGHNGLFLFSVL 180

Query: 181 CFVYSLVSCLLGRTPHLPLVADAAGRQI 209
           CFVYSL SCLLGRTPHLPLVADAAGRQI
Sbjct: 181 CFVYSLASCLLGRTPHLPLVADAAGRQI 208

[Close]
BLAST of Lag0035176 vs. ExPASy TrEMBL
Match: A0A6J1HBN6 (Protein TIC 20 OS=Cucurbita moschata OX=3662 GN=LOC111461378 PE=3 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 1.5e-94
Identity = 178/208 (85.58%), Postives = 192/208 (92.31%), Query Frame = 0 

Query: 1   MASTSIPLINLSTKSAIKASLIPHFSALTSRHRSSLPLLPSKPAKCPAIRMSYNPTPATD 60
           MAS SIPL+NLS K AIKA+LIPH+SA+ SRH  SLPLLPSKPAK PAIRMSYNPTPATD
Sbjct: 3   MASCSIPLLNLSIKPAIKAALIPHYSAIKSRHCFSLPLLPSKPAKSPAIRMSYNPTPATD 62

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPALGLAFQPLLPILSLYRSIPHSSFVAFFALYLG 120
           RLISAVAYTLPFFNSLQYGRFLFAQYP LGLAF+PLLPIL LYRSIPHSSF+AFFA YLG
Sbjct: 63  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG 122

Query: 121 VVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGLGFQIMVWGHNALFVFSVL 180
           VVRNPSFS Y RFNSMQA+TLDVLLVVPLL+QRILSPG+ GLGFQIM W HN LFVFSVL
Sbjct: 123 VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGFQIMAWSHNGLFVFSVL 182

Query: 181 CFVYSLVSCLLGRTPHLPLVADAAGRQI 209
           CF+YS+VSC+LGRTPHLP+VADAAGRQ+
Sbjct: 183 CFLYSVVSCILGRTPHLPIVADAAGRQV 210

[Close]
BLAST of Lag0035176 vs. ExPASy TrEMBL
Match: A0A0A0LXS7 (Protein TIC 20 OS=Cucumis sativus OX=3659 GN=Csa_1G654890 PE=3 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 5.6e-94
Identity = 179/208 (86.06%), Postives = 192/208 (92.31%), Query Frame = 0 

Query: 1   MASTSIPLINLSTKSAIKASLIPHFSALTSRHRSSLPLLPSKPAKCPAIRMSYNPTPATD 60
           MASTSIPL+NLS K++I+A+L PH SA+ SRH SSLP LPSK  K   IRMSYNPTPATD
Sbjct: 1   MASTSIPLLNLSAKTSIRAALFPHSSAIKSRHHSSLPFLPSKSPKFTPIRMSYNPTPATD 60

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPALGLAFQPLLPILSLYRSIPHSSFVAFFALYLG 120
           RLISAVAYTLPFFNSLQYGRFLFAQYPALG AF+PLLPIL LYRSIPHSSFVAFFALYLG
Sbjct: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPALGFAFEPLLPILGLYRSIPHSSFVAFFALYLG 120

Query: 121 VVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGLGFQIMVWGHNALFVFSVL 180
           VVRNPSF+HYVRFNSMQAVTLDVLLVVPLLIQRILSPGR G+GF+IMVW HN LFVFSVL
Sbjct: 121 VVRNPSFNHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRSGIGFKIMVWSHNGLFVFSVL 180

Query: 181 CFVYSLVSCLLGRTPHLPLVADAAGRQI 209
           CF+YS+VSCLLGRTPHLP+VADAAGRQI
Sbjct: 181 CFLYSVVSCLLGRTPHLPIVADAAGRQI 208

[Close]
BLAST of Lag0035176 vs. TAIR 10
Match: AT2G47840.1 (Uncharacterised conserved protein ycf60 )

HSP 1 Score: 238.0 bits (606), Expect = 6.6e-63
Identity = 116/169 (68.64%), Postives = 139/169 (82.25%), Query Frame = 0 

Query: 41  SKPAKCPAIRMSYNPTPATDRLISAVAYTLPFFNSLQYGRFLFAQYPALGLAFQPLLPIL 100
           S+ A     R SY PTPAT+R+IS  +Y LPFFNSLQYGRFLFAQYP LGL F+P+ PIL
Sbjct: 40  SRRATALTTRASYTPTPATERVISIASYALPFFNSLQYGRFLFAQYPRLGLLFEPIFPIL 99

Query: 101 SLYRSIPHSSFVAFFALYLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGR- 160
           +LYRS+P++SFVAFF LYLGVVRN SFS YVRFN+MQAVTLDVLL VP+L+ RIL PG+ 
Sbjct: 100 NLYRSVPYASFVAFFGLYLGVVRNTSFSRYVRFNAMQAVTLDVLLAVPVLLTRILDPGQG 159

Query: 161 GGLGFQIMVWGHNALFVFSVLCFVYSLVSCLLGRTPHLPLVADAAGRQI 209
           GG G + M+WGH  +FVFS +CFVY +VS LLG+TP++P VADAAGRQ+
Sbjct: 160 GGFGMKAMMWGHTGVFVFSFMCFVYGVVSSLLGKTPYIPFVADAAGRQL 208

[Close]
BLAST of Lag0035176 vs. TAIR 10
Match: AT5G55710.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast thylakoid membrane, chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: Uncharacterised conserved protein ycf60 (TAIR:AT2G47840.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 147.9 bits (372), Expect = 9.0e-36
Identity = 71/151 (47.02%), Postives = 102/151 (67.55%), Query Frame = 0 

Query: 58  ATDRLISAVAYTLPFFNSLQYGRFLFAQYPALGLAFQPLLPILSLYRSIPHSSFVAFFAL 117
           A+DR+ISAV Y  PFF+ +QYG+F+  QY    +  QPL P +  ++S P + F+ F  L
Sbjct: 59  ASDRIISAVCYFYPFFDGIQYGKFIITQYQPFQILIQPLFPAIRAFKSFPFNGFLIFITL 118

Query: 118 YLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGLGFQIMVWGHNALFVF 177
           Y  VVRNP+FS YVRFN+MQA+ LDVLL+ P L++R  +P R G G  +++   + +F+F
Sbjct: 119 YFVVVRNPNFSRYVRFNTMQAIVLDVLLIFPDLLERSFNP-RDGFGLDVVMSLDSTVFLF 178

Query: 178 SVLCFVYSLVSCLLGRTPHLPLVADAAGRQI 209
            ++  +Y   +CL G TP LPLVA+AA RQ+
Sbjct: 179 LLVSLIYGFSACLFGLTPRLPLVAEAADRQV 208

[Close]
BLAST of Lag0035176 vs. TAIR 10
Match: AT1G04940.1 (translocon at the inner envelope membrane of chloroplasts 20 )

HSP 1 Score: 43.5 bits (101), Expect = 2.4e-04
Identity = 54/224 (24.11%), Postives = 89/224 (39.73%), Query Frame = 0 

Query: 3   STSIPLINLSTKSAI-----KASLIPHFSALTSRHRSSLPLLPSKPAKCPAIRMSYNPTP 62
           S  +PL  LS  S++     + SL      L  R ++ L    SK      +  S+   P
Sbjct: 61  SRGVPLSYLSASSSLLLNGEQGSLSGTLPVLPVRRKTLLTPRASKD-----VPSSFRFPP 120

Query: 63  ATD------RLISAVAYTLPFFNSLQYGRFLFAQYPALGLAFQPLLPILSLYRSIPHSSF 122
            T       R ++ + Y +P   +  Y    +  +P L        P L     +P    
Sbjct: 121 MTKKPQWWWRTLACLPYLMPLHETWMYAETAYHLHPFLEDFEFLTYPFLGAIGRLPSWFL 180

Query: 123 VA-FFALYLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGLGFQIMVWG 182
           +A FF  YLG+VR   + H+ RF+ +  + L++ L V   + + +  G        + WG
Sbjct: 181 MAYFFVAYLGIVRRKEWPHFFRFHVVMGMLLEIALQVIGTVSKWMPLG--------VYWG 240

Query: 183 HNALFVFSVLCFVY------SLVSCLLGRTPHLPLVADAAGRQI 209
              +  ++ + F Y      S+   L G    +P V DAA  QI
Sbjct: 241 KFGMHFWTAVAFAYLFTVLESIRCALAGMYADIPFVCDAAYIQI 271

The following BLAST results are available for this feature:
BLAST of Lag0035176 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038879337.11.7e-9788.94protein TIC 20-II, chloroplastic [Benincasa hispida][more]
XP_022987499.14.7e-9586.06protein TIC 20-II, chloroplastic-like [Cucurbita maxima][more]
XP_023005745.11.8e-9487.02protein TIC 20-II, chloroplastic-like isoform X2 [Cucurbita maxima][more]
XP_023005732.11.8e-9487.02protein TIC 20-II, chloroplastic-like isoform X1 [Cucurbita maxima] >XP_02300574... [more]
XP_023516082.12.3e-9485.58protein TIC 20-II, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
BLAST of Lag0035176 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O822519.3e-6268.64Protein TIC 20-II, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-II PE=... [more]
Q9FM671.3e-3447.02Protein TIC 20-v, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-V PE=2 ... [more]
P733871.2e-2134.44Tic20 family protein OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 ... [more]
Q9ZST82.5e-0626.92Protein TIC 20, chloroplastic OS=Pisum sativum OX=3888 GN=TIC20 PE=1 SV=1[more]
BLAST of Lag0035176 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1JJ222.3e-9586.06Protein TIC 20 OS=Cucurbita maxima OX=3661 GN=LOC111485044 PE=3 SV=1[more]
A0A6J1KY868.7e-9587.02Protein TIC 20 OS=Cucurbita maxima OX=3661 GN=LOC111498634 PE=3 SV=1[more]
A0A6J1KY918.7e-9587.02Protein TIC 20 OS=Cucurbita maxima OX=3661 GN=LOC111498634 PE=3 SV=1[more]
A0A6J1HBN61.5e-9485.58Protein TIC 20 OS=Cucurbita moschata OX=3662 GN=LOC111461378 PE=3 SV=1[more]
A0A0A0LXS75.6e-9486.06Protein TIC 20 OS=Cucumis sativus OX=3659 GN=Csa_1G654890 PE=3 SV=1[more]
BLAST of Lag0035176 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G47840.16.6e-6368.64Uncharacterised conserved protein ycf60 [more]
AT5G55710.19.0e-3647.02FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT1G04940.12.4e-0424.11translocon at the inner envelope membrane of chloroplasts 20 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005691Chloroplast protein import component Tic20PFAMPF16166TIC20coord: 61..204
e-value: 2.6E-45
score: 154.4
IPR005691Chloroplast protein import component Tic20PANTHERPTHR33510PROTEIN TIC 20-II, CHLOROPLASTICcoord: 49..208
NoneNo IPR availablePANTHERPTHR33510:SF5PROTEIN TIC 20-II, CHLOROPLASTICcoord: 49..208

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0035176.1Lag0035176.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0009706 chloroplast inner membrane
cellular_component GO:0016021 integral component of membrane