Lag0035163 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0035163 vs. NCBI nr
Match: BBN69274.1 (TatD related DNase [Prunus dulcis])

HSP 1 Score: 144.1 bits (362), Expect = 2.3e-30
Identity = 89/273 (32.60%), Postives = 135/273 (49.45%), Query Frame = 0

Query: 72  YVNFYKKKLEFLHNDQCFGLILFDFLSIFVKWNSEVVR------FGTIFSVAGLRRI--- 131
           ++ F    + FL   + + L L   L +F   +   +        G  FS+  L  +   
Sbjct: 438 HLQFADDTIFFLDGKEEYWLNLLQMLKLFCDVSGMKINKAKSCILGINFSIETLNNMAGS 497

Query: 132 WG-----WLDTYLGPPLNGKPKSSPFLDPVVEKIKKRLHTWGSTSLRRKKAHSFSGHTIK 191
           WG     W   YLG PL G P++  F +PV++K++KRL  W    L +    +     + 
Sbjct: 498 WGCEVGCWPMVYLGLPLGGNPRALNFWNPVLDKVEKRLQRWKRACLSKGGRLTLIQAVLS 557

Query: 192 --PAHILSLPLQGSHKVTKKIEKLFQNFLCRGNNEFKGIHLLEWERVKVPIEEGGLGIMG 251
             P++ +SL  +    VT K+E+L +NFL  G  E K  HL+ W RV    EEGGLGI  
Sbjct: 558 SIPSYYMSL-FKMPIGVTAKVEQLMRNFLWEGVEEGKNCHLVRWVRVTKSKEEGGLGIGS 617

Query: 252 IQQKNSSLLAKWIWRFHKERGALWRRVIGSKYGSIHFGLKPDKISKL-IQSPWKSIESTK 311
           ++++N +L AKW+WRF  E  +LW R+I SKYG    G    +I K+  ++PW+ I    
Sbjct: 618 LRERNEALRAKWLWRFPLETNSLWHRIIKSKYGLDSNGWDTKQIDKVSCRNPWREISKGY 677

Query: 312 FLVYSNGSIKVGDGKNTPFWKDTWLENLSLQRL 328
                     VG+G+   FW+D WL+   L+ L
Sbjct: 678 ISFLQCCRFSVGNGEKIRFWEDFWLKEGILKDL 709

BLAST of Lag0035163 vs. NCBI nr
Match: TQD93576.1 (hypothetical protein C1H46_020784 [Malus baccata])

HSP 1 Score: 142.9 bits (359), Expect = 5.1e-30
Identity = 84/208 (40.38%), Postives = 115/208 (55.29%), Query Frame = 0

Query: 123 WG-----WLDTYLGPPLNGKPKSSPFLDPVVEKIKKRLHTWGSTSLRRKKAHSFSGHTI- 182
           WG     W   YLG PL G+P++  F DPVVEK++KRL +W    L R    +     + 
Sbjct: 34  WGCEVGRWPIKYLGLPLGGRPRALKFWDPVVEKMEKRLQSWKKAFLSRGGRLTLIQSVLG 93

Query: 183 -KPAHILSLPLQGSHKVTKKIEKLFQNFLCRGNNEFKGIHLLEWERVKVPIEEGGLGIMG 242
             P + +SL  +    V  ++EKL + FL  G  E K  HL++WE V    EEGGLG+  
Sbjct: 94  SLPIYYMSL-FKIPCGVIGRLEKLMKGFLWEGVEEGKKNHLVKWEIVIKSKEEGGLGVGN 153

Query: 243 IQQKNSSLLAKWIWRFHKERGALWRRVIGSKYGSIHFG---LKPDKISKLIQSPWKSIES 302
           ++ +N +LLAKW+WRF KE  +LW +VI SKYG  + G     P + S   +SPWK I S
Sbjct: 154 LRNRNEALLAKWLWRFPKEPHSLWHKVIRSKYGLQNNGWNAFPPMRGSS--RSPWKDISS 213

Query: 303 TKFLVYSNGSIKVGDGKNTPFWKDTWLE 321
              L       +VG+G+   FW+D WLE
Sbjct: 214 GSQLFLHCCKFEVGNGERVRFWEDGWLE 238

BLAST of Lag0035163 vs. NCBI nr
Match: VVA30963.1 (Hypothetical predicted protein, partial [Prunus dulcis])

HSP 1 Score: 141.7 bits (356), Expect = 1.1e-29
Identity = 85/237 (35.86%), Postives = 123/237 (51.90%), Query Frame = 0

Query: 111 GTIFSVAGLRRI---WG-----WLDTYLGPPLNGKPKSSPFLDPVVEKIKKRLHTWGSTS 170
           G  FS+  L  +   WG     W   YLG PL G P++  F +PV +K++KRL  W    
Sbjct: 49  GINFSIEALNNMAGSWGCEVGCWPMVYLGLPLRGNPRALNFWNPVKDKVEKRLQKWKRAC 108

Query: 171 LRRKKAHSFSGHTIK--PAHILSLPLQGSHKVTKKIEKLFQNFLCRGNNEFKGIHLLEWE 230
           L +    +     +   P++ +SL  +    V  K+E+L +NFL  G  E K  HL+ WE
Sbjct: 109 LSKGGRLTLIQAVLSSIPSYYMSL-FKMPTGVAAKVEQLMRNFLWEGLEEGKKCHLVRWE 168

Query: 231 RVKVPIEEGGLGIMGIQQKNSSLLAKWIWRFHKERGALWRRVIGSKYGSIHFGLKPDKIS 290
           RV    EEGGLGI  ++++N +L AKW+WRF  E  +LW R+I SKYG    G    +I 
Sbjct: 169 RVTKSKEEGGLGIGSLRERNEALRAKWLWRFPLETNSLWHRIIKSKYGIDSNGWDTKQIV 228

Query: 291 KL-IQSPWKSIESTKFLVYSNGSIKVGDGKNTPFWKDTWLENLSLQ----RLRTLER 333
           K+  ++PW+ I              VG+G+   FW+D WL+   L+    RL +L R
Sbjct: 229 KVSCRNPWREISKGYNSFLQCCRFSVGNGEKIRFWEDLWLKEGILKDLFPRLSSLSR 284

BLAST of Lag0035163 vs. NCBI nr
Match: XP_031736165.1 (uncharacterized protein LOC101204937 isoform X4 [Cucumis sativus])

HSP 1 Score: 140.2 bits (352), Expect = 3.3e-29
Identity = 64/72 (88.89%), Postives = 67/72 (93.06%), Query Frame = 0

Query: 17 RYGRFRPW-FPLSRKVTGWQILWWQYAQQSVLSDVRKKLKKCSWRYLGQRLIYRRRYVNF 76
          RYGRFRPW FPLSRKV GWQ+LWW YAQQSVL DVRK+LKKCSWRYLGQRL+YRRRYV F
Sbjct: 3  RYGRFRPWCFPLSRKVKGWQMLWWHYAQQSVLLDVRKRLKKCSWRYLGQRLLYRRRYVEF 62

Query: 77 YKKKLEFLHNDQ 88
          YKKKLEFLHNDQ
Sbjct: 63 YKKKLEFLHNDQ 74

BLAST of Lag0035163 vs. NCBI nr
Match: RVW39368.1 (LINE-1 retrotransposable element ORF2 protein [Vitis vinifera])

HSP 1 Score: 140.2 bits (352), Expect = 3.3e-29
Identity = 84/214 (39.25%), Postives = 112/214 (52.34%), Query Frame = 0

Query: 121  RIWGWLDTYLGPPLNGKPKSSPFLDPVVEKIKKRLHTWGSTSLRRKKAHSFSGHTIK--- 180
            R+  W  +YLG PL G PK+  F DPVVE+I +RL  W       KKA+   G  I    
Sbjct: 1831 RVSEWPLSYLGLPLGGNPKTIGFWDPVVERISRRLDGW-------KKAYLSLGGRITLIQ 1890

Query: 181  ------PAHILSLPLQGSHKVTKKIEKLFQNFLCRGNNEFKGIHLLEWERVKVPIEEGGL 240
                  P++ LSL  +    +  KIEK+ +NFL  G  E K  HL+ WE V  P E GGL
Sbjct: 1891 SCLSHIPSYFLSL-FKIPASIASKIEKMQRNFLWSGAGEGKKDHLVRWEVVSRPKESGGL 1950

Query: 241  GIMGIQQKNSSLLAKWIWRFHKERGALWRRVIGSKYGSIHFGLKPDKISKLI-QSPWKSI 300
            G   I  +N +LL KW+WRF +ER  LW +VIGS YG+   G   + + +   + PWK+I
Sbjct: 1951 GFGKISLRNIALLGKWLWRFPRERSGLWHKVIGSIYGTHPNGWDANMVVRWSHRCPWKAI 2010

Query: 301  ESTKFLVYSNGSIKVGDGKNTPFWKDTWLENLSL 325
                        + VG+G+   FW+D W  N SL
Sbjct: 2011 AQVFQEFSPFVRLVVGNGERIRFWEDLWWGNQSL 2036

BLAST of Lag0035163 vs. ExPASy TrEMBL
Match: A0A5H2XQW2 (TatD related DNase OS=Prunus dulcis OX=3755 GN=Prudu_862S001100 PE=4 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 1.1e-30
Identity = 89/273 (32.60%), Postives = 135/273 (49.45%), Query Frame = 0

Query: 72  YVNFYKKKLEFLHNDQCFGLILFDFLSIFVKWNSEVVR------FGTIFSVAGLRRI--- 131
           ++ F    + FL   + + L L   L +F   +   +        G  FS+  L  +   
Sbjct: 438 HLQFADDTIFFLDGKEEYWLNLLQMLKLFCDVSGMKINKAKSCILGINFSIETLNNMAGS 497

Query: 132 WG-----WLDTYLGPPLNGKPKSSPFLDPVVEKIKKRLHTWGSTSLRRKKAHSFSGHTIK 191
           WG     W   YLG PL G P++  F +PV++K++KRL  W    L +    +     + 
Sbjct: 498 WGCEVGCWPMVYLGLPLGGNPRALNFWNPVLDKVEKRLQRWKRACLSKGGRLTLIQAVLS 557

Query: 192 --PAHILSLPLQGSHKVTKKIEKLFQNFLCRGNNEFKGIHLLEWERVKVPIEEGGLGIMG 251
             P++ +SL  +    VT K+E+L +NFL  G  E K  HL+ W RV    EEGGLGI  
Sbjct: 558 SIPSYYMSL-FKMPIGVTAKVEQLMRNFLWEGVEEGKNCHLVRWVRVTKSKEEGGLGIGS 617

Query: 252 IQQKNSSLLAKWIWRFHKERGALWRRVIGSKYGSIHFGLKPDKISKL-IQSPWKSIESTK 311
           ++++N +L AKW+WRF  E  +LW R+I SKYG    G    +I K+  ++PW+ I    
Sbjct: 618 LRERNEALRAKWLWRFPLETNSLWHRIIKSKYGLDSNGWDTKQIDKVSCRNPWREISKGY 677

Query: 312 FLVYSNGSIKVGDGKNTPFWKDTWLENLSLQRL 328
                     VG+G+   FW+D WL+   L+ L
Sbjct: 678 ISFLQCCRFSVGNGEKIRFWEDFWLKEGILKDL 709

BLAST of Lag0035163 vs. ExPASy TrEMBL
Match: A0A540M4H0 (zf-RVT domain-containing protein OS=Malus baccata OX=106549 GN=C1H46_020784 PE=4 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 2.5e-30
Identity = 84/208 (40.38%), Postives = 115/208 (55.29%), Query Frame = 0

Query: 123 WG-----WLDTYLGPPLNGKPKSSPFLDPVVEKIKKRLHTWGSTSLRRKKAHSFSGHTI- 182
           WG     W   YLG PL G+P++  F DPVVEK++KRL +W    L R    +     + 
Sbjct: 34  WGCEVGRWPIKYLGLPLGGRPRALKFWDPVVEKMEKRLQSWKKAFLSRGGRLTLIQSVLG 93

Query: 183 -KPAHILSLPLQGSHKVTKKIEKLFQNFLCRGNNEFKGIHLLEWERVKVPIEEGGLGIMG 242
             P + +SL  +    V  ++EKL + FL  G  E K  HL++WE V    EEGGLG+  
Sbjct: 94  SLPIYYMSL-FKIPCGVIGRLEKLMKGFLWEGVEEGKKNHLVKWEIVIKSKEEGGLGVGN 153

Query: 243 IQQKNSSLLAKWIWRFHKERGALWRRVIGSKYGSIHFG---LKPDKISKLIQSPWKSIES 302
           ++ +N +LLAKW+WRF KE  +LW +VI SKYG  + G     P + S   +SPWK I S
Sbjct: 154 LRNRNEALLAKWLWRFPKEPHSLWHKVIRSKYGLQNNGWNAFPPMRGSS--RSPWKDISS 213

Query: 303 TKFLVYSNGSIKVGDGKNTPFWKDTWLE 321
              L       +VG+G+   FW+D WLE
Sbjct: 214 GSQLFLHCCKFEVGNGERVRFWEDGWLE 238

BLAST of Lag0035163 vs. ExPASy TrEMBL
Match: M5WZA0 (Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa020022mg PE=4 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 3.2e-30
Identity = 85/237 (35.86%), Postives = 124/237 (52.32%), Query Frame = 0

Query: 111  GTIFSVAGLRRI---WG-----WLDTYLGPPLNGKPKSSPFLDPVVEKIKKRLHTWGSTS 170
            G  FS+  L  +   WG     W   YLG PL G P++  F +PVV+K++KRL  W    
Sbjct: 858  GINFSIEALNNMAGSWGCEVGCWPMVYLGLPLEGNPRALNFWNPVVDKVEKRLQKWKRAC 917

Query: 171  LRRKKAHSFSGHTIK--PAHILSLPLQGSHKVTKKIEKLFQNFLCRGNNEFKGIHLLEWE 230
            L +    +     +   P++ +SL  +    V  K+E+L +NFL  G  E K  HL+ WE
Sbjct: 918  LSKGGRLTLIQAVLSSIPSYYMSL-FKMPIGVAAKVEQLMRNFLWEGLEEGKKCHLVRWE 977

Query: 231  RVKVPIEEGGLGIMGIQQKNSSLLAKWIWRFHKERGALWRRVIGSKYGSIHFGLKPDKIS 290
            RV    EEGGLGI  +++++ +L AKW+WRF  E  +LW R+I SKYG    G    +I 
Sbjct: 978  RVTKSKEEGGLGIGSLRERDEALRAKWLWRFPLETNSLWHRIIKSKYGIDSNGWDTKQID 1037

Query: 291  KL-IQSPWKSIESTKFLVYSNGSIKVGDGKNTPFWKDTWLENLSLQ----RLRTLER 333
            K+  ++PW+ I              VG+G+   FW+D WL+   L+    RL +L R
Sbjct: 1038 KVSCRNPWREISKGYNSFLQCCRFSVGNGEKIRFWEDLWLKEGILKDLFPRLSSLSR 1093

BLAST of Lag0035163 vs. ExPASy TrEMBL
Match: M5VS59 (Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa016504mg PE=4 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 3.2e-30
Identity = 85/237 (35.86%), Postives = 123/237 (51.90%), Query Frame = 0

Query: 111 GTIFSVAGLRRI---WG-----WLDTYLGPPLNGKPKSSPFLDPVVEKIKKRLHTWGSTS 170
           G  FS   L  +   WG     W   YLG PL G P++  F +PV++K++KRL  W    
Sbjct: 714 GINFSTEALNNMAGSWGCEVGCWPMVYLGLPLGGNPRALNFWNPVMDKVEKRLQKWKRAC 773

Query: 171 LRRKKAHSFSGHTIK--PAHILSLPLQGSHKVTKKIEKLFQNFLCRGNNEFKGIHLLEWE 230
           L +    +     +   P++ +SL  +    V  K+E+L +NFL  G  E K  HL+ WE
Sbjct: 774 LSKGGRLTLIQAVLSSIPSYYMSL-FKMPIGVAAKVEQLMRNFLWEGLEEGKNCHLVRWE 833

Query: 231 RVKVPIEEGGLGIMGIQQKNSSLLAKWIWRFHKERGALWRRVIGSKYGSIHFGLKPDKIS 290
           RV    EEGGLGI  ++++N +L AKW+WRF  E  +LW R+I SKYG    G    +I 
Sbjct: 834 RVTKSKEEGGLGIGSLRERNEALRAKWLWRFPLEPNSLWHRIIKSKYGIDSNGWDTKQID 893

Query: 291 KL-IQSPWKSIESTKFLVYSNGSIKVGDGKNTPFWKDTWLENLSLQ----RLRTLER 333
           K+  ++PW+ I              VG+G+   FW+D WL+   L+    RL +L R
Sbjct: 894 KVSCRNPWREISKGYNSFLQCCRFSVGNGEKIRFWEDLWLKEGILKDLFPRLSSLSR 949

BLAST of Lag0035163 vs. ExPASy TrEMBL
Match: M5X4S0 (Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa021750mg PE=4 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 4.2e-30
Identity = 82/222 (36.94%), Postives = 118/222 (53.15%), Query Frame = 0

Query: 123 WG-----WLDTYLGPPLNGKPKSSPFLDPVVEKIKKRLHTWGSTSLRRKKAHSFSGHTIK 182
           WG     W   YLG PL G P++  F +PV+EK++KRL  W    L +    +     + 
Sbjct: 478 WGCEVGCWPMVYLGLPLGGNPRALNFWNPVMEKVEKRLQKWKRACLSKGGRLTLIQAVLS 537

Query: 183 --PAHILSLPLQGSHKVTKKIEKLFQNFLCRGNNEFKGIHLLEWERVKVPIEEGGLGIMG 242
             P++ +SL  +    V  K+E+L +NFL  G  E K  HL+ WERV    EEGGLGI  
Sbjct: 538 SIPSYYMSL-FKMPIGVAAKVEQLMRNFLWEGLEEGKKCHLVRWERVTKSKEEGGLGIGS 597

Query: 243 IQQKNSSLLAKWIWRFHKERGALWRRVIGSKYGSIHFGLKPDKISKL-IQSPWKSIESTK 302
           ++++N +L AKW+WRF  E  +LW R+I SKYG    G    +I K+  ++PW+ I    
Sbjct: 598 LRERNEALRAKWLWRFPLETNSLWHRIIKSKYGIDSNGWDTKRIDKVSCRNPWREISKGY 657

Query: 303 FLVYSNGSIKVGDGKNTPFWKDTWLENLSLQ----RLRTLER 333
                     VG+G+   FW+D WL+   L+    RL +L R
Sbjct: 658 NSFLQCCRFSVGNGEKIRFWEDLWLKEGILKDLFPRLSSLSR 698

BLAST of Lag0035163 vs. TAIR 10
Match: AT5G24740.2 (Protein of unknown function (DUF1162) )

HSP 1 Score: 95.1 bits (235), Expect = 1.1e-19
Identity = 52/82 (63.41%), Postives = 58/82 (70.73%), Query Frame = 0

Query: 17  RYGRFRPW-FPLSRKVTGWQILWWQYAQQSVLSDVRKKLKKCSWRYLGQRLIYRRRYVNF 76
           RYGR+RP    LSRK  GWQ LWW YAQ SVLSDVRKKL K SWR+L QR+  RRRY+NF
Sbjct: 308 RYGRYRPCSTSLSRKPPGWQKLWWHYAQNSVLSDVRKKLWKTSWRFLEQRMRIRRRYINF 367

Query: 77  YKKKLEFLHNDQCFGLILFDFL 98
           YK KL+ L  +Q    ILF  L
Sbjct: 368 YKIKLDLLLREQ--ARILFSSL 387

BLAST of Lag0035163 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 68.2 bits (165), Expect = 1.5e-11
Identity = 39/134 (29.10%), Postives = 66/134 (49.25%), Query Frame = 0

Query: 192 VTKKIEKLFQNFLCRGNNEFKGIHLLEWERVKVPIEEGGLGIMGIQQKNSSLLAKWIWRF 251
           V K+I  +  +F  R   E KG+H   W+ +     EGG+G   I+  N +LL K +WR 
Sbjct: 17  VCKQIISVLADFWWRNKQEAKGMHWKAWDHLSCYKAEGGIGFKDIEAFNLALLGKQMWRM 76

Query: 252 HKERGALWRRVIGSKYGSIHFGLKPDKISKLIQS----PWKSIESTKFLVYSNGSIKVGD 311
                +L  +V  S+Y   H   K D ++  + S     WKSI +++ ++       VG+
Sbjct: 77  LSRPESLMAKVFKSRY--FH---KSDPLNAPLGSRPSFVWKSIHASQEILRQGARAVVGN 136

Query: 312 GKNTPFWKDTWLEN 322
           G++   W+  WL++
Sbjct: 137 GEDIIIWRHKWLDS 145

BLAST of Lag0035163 vs. TAIR 10
Match: AT5G24740.1 (Protein of unknown function (DUF1162) )

HSP 1 Score: 50.4 bits (119), Expect = 3.2e-06
Identity = 24/34 (70.59%), Postives = 25/34 (73.53%), Query Frame = 0

Query: 17  RYGRFRPW-FPLSRKVTGWQILWWQYAQQSVLSD 50
           RYGR+RP    LSRK  GWQ LWW YAQ SVLSD
Sbjct: 308 RYGRYRPCSTSLSRKPPGWQKLWWHYAQNSVLSD 341