Lag0034859 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0034859
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionlysine-specific demethylase JMJ30-like
Locationchr3: 11743030 .. 11747127 (+)
RNA-Seq ExpressionLag0034859
SyntenyLag0034859
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTCATCGGAACCCAACCAAAACCCTTCTTCATACCATTTCGAAACCCCTCTTCTCGACGCCGAGTCGCCGTCGCTGCTCCACTCCATCTCCGAGCACGGTGGCTACGCCTACGTCAGCATGGCGGCGCTCGCGGCTTCCGGGGACTCATGCGCTGCCGAGGCCGCCGGCGAGATGGCCTGGGAGCAGCTTCACTCTGGCCCCTGGCACTCTGTTCTCCCTATCTGGCGCGATGCCTACTCCATGGCTTGCCTCTACATGGCGAGGTTCCATTTTGCTGGCGGCGAGTTTAAGGAGGCTCTTAGGGTCTTGGATATGGGGCTGATCATGGGCGGCCCGCTTTTGAGGAGTGATTTGGATTCTGCCATTGCGAAGGTCTCTGCTGAGGCGAGGATTGTTAGGGTTTTGGATGTTAACCAGAAGGATGCCCAGTTGGAGCCTCCGTTGGGTTGTGAGGAGCGTAATAAGGAGGTGAGGTTCTTTTTTTTCTCTTTGTGTGTGTGTTTGTGTTCTTAAAAGTTCTTGCTGTTGCTGATTCTAATGTTTTGGAGGAATTTCTTGCTGTTTTTTGGCTATGGATACTTTCTTAGTTCGTTTTATATGATGCTGATTTCTGTGGAACTGATTGTTTTAAATCATTCTTATAATGGGAAATATGTTTGTTTGTTTGTTTTCCCTTTTTCCATTCATTCATTTGTGAAACATTTGCTGTTTTCCGTGCTCTGTTTTAAGCTTTAAGTTTCTTTTCTGTAATTATTTGTGGGAACTTGGGACTTGGGATGTGTTCAGCGTGAGTGGTTTCATTTAAAGAGGAATCGGATTCAATATAATTTTGATAGAAAGCCCATTTTAAGAAAACGAAGATGGAAGTCTGCAAGAATATAGACTAGCCTTACACTGCAGAAACGAGAACTAAATGGATTTGTTTTTCAAATCGTGGTGGATCTTTTTTTGCCTTTAGCAAATGAAATATTTCGGTTGCATTTTTTTCTTAAATGTTTACTAATACCATTCTGGAATGGCTTTATCATTTCTACCTCCAGACCCGTAGAAATGTTGACTATGAGTTACCACGAGGGAGCCCAGGAGCTATCTTGGATTCAAACAACCAACTAGTGTGCTTGCTAAGTTAATGCTTAACGGTGTTCTTGTTCAAGTTTGATTCATGATAAGGGGAACTGTTGACGTAAACTGAACACCCATTTTCTATTTTTTGCATTTTCACTGGCAGGCTCAATGATGGATTTGATTACGGTTGTTGAGAGTTATATAATTCTAAATCTTATTTCTTTTGGATAAATATATGTTAGAAGGTGAACATGATTTAAAGATCTTTTAGTGACTTGGAGATCATTTGAGAATATGATTTTCTCAAGGTAGAGAACTCCTCTATCCACCATTCTAAATTCTATAAAAATAATGATAGTGTTCTATCTGAGGAACTTAGGATAATATTCATAACAGTCCTTGCACTGGATAATTCTTATATTACCAATTAGTTCCCAAAGCTCAAAATTTCCTATGAAGTCCTTCTAAGACTCTCCCATTCCATTTTAGCCAAAGCATAGAGATAGAGTAGTAGTCTTATTAAAATTTAGTGACAATGTGACAAGATTCTTGTGTTGCCTTTGAACTGATGGCTACAGAGTATCTGAAGTAAGAACTCCTTAACACTTCTACCAGTAAGAACTCCTTAACACTTCTAGGGAGAATCACTTGAAACCCAAAACAGTTAAGAAGCTTCTGCCACAAACTTATGGAGAATGAACACCAAGGAATAGATCCAAACAATTTTTTTTTCTATCATGAGTATCCATGCGAAGAAAAATATCTAGGATTCCTTGCTTCAATTCTATCATGAGTATCAATGCCACAGAGGAATAGAGTCCAAACAAAAAAATTTCTTTCTTTCAAAATGACCTTACTCAACTTGAAAGAATAGCATAAGCCACAACTGAGAGATTTTGAGAAAGGGACTTGCATGAGAACTTCCCTGAAATTCAAATCATATTGTATCTCTGCTTTATTAAGTATTTTGATTTTGAGCCCTTACCTAGCTTTAAAAACTTTTCCCTCTTCCATCATTTCTTTGTATGGTTGTGCTAATTTTTGTTTTCTTGGTACTACGCGGTGTTGACATAATCATATAATCCATGTGTCCAAGGGAATTCCAAAATCTATTCTAACAAGTAATATGTGATGCCTTTAACGTATAGGTTCCTTGGAATTTGCCTGTAAAGGCTCTCAGTAATAAGATGGTTGCAAAGATGTCAGCTTTATCTCTAGAGGGATTCTTGCGTGAATATTTCCAACCGGGTTTCCCAGTTATTATTAGTGATGGTATGGCTCATTGGCCAGCTAGGACAAAGTGGAAGAGCATGGATTACTTACAAAAGGTTGCTGGTGACCGTACAATCCCAGTTGAGGTGATTTATTTATCTATTCATTTGTTTATTATGAATATAATGGACAATCTTGGATCTTGATAACGTGTGTCCCAAATTTCATATTAAAATTTTTCTCAACTATTACTCCGTAGTTGCTTGTTACTGGAATAACAAATATAAAAGTTGCCTTTTTGAGATGTTTATTTCTTTTCTTGTTGTTTATATATTTTCGTCACTTTTCTTCCTATTGTAGCTTAACTCCTCCTGCTGTCTCTCCTGCTGACTTGATATTGGAACTGATAGATTTTCCACCCGTGAACTGAAGACTTGTAAAGCTTTTATGCTTTTCAGGTTGGGAAGAACTATTTACGCCCAGAATGGAAACAAGAGCTTATCACATTTTCCGAATTTCTCGGCAGGATTCAGTTGAATGACTGTTCTGATGATATTACATATCTTGCTCAACATCCATTATTTGACCAGGCATGTATCGTTGTATAACTTTATCTGCTTATCATTTTATGTGCATGTATAGGCATATTTATAAATCTTAACCGGTAGATGCTTTGAAGAACCTAGTGCTAACCTTCAGCATTTCGTAGTGCAAATATTATTCAATACTTTTCTTTCTTTCTTCAAAGACAGATAGATGAGCTCCAGAAGGACATTTGTATTCCTGATTACTGTTTTGTTGGTGGCGGAGAGTTAAGATCTCTTAATGCTTGGTTTGGCCCACCTGGGACAGTAACACCTTTACACCATGATCCTCATCACAATATATTAGCTCAGGTTTGTACTAACAGATGATTTCTTAAAATGATTAAACTGGAAAGCATTGTAACTTTTTGAGTTTATTTCTCATTTTGATTTCCTGGATCTTTTTCTCTACTGGCATGACATATATTTACATAATCACGTTTATCTGAAAGAGGGTATGAATAATGATGCTTTGCCCGCTTGTTAAAAAAAAACAAGATGCTGCAAGTTTTTACAACATGAAACTGCTGTTAGTTTAATACTTAACAGTTAACACTTTTGATACTAAGACGACCTTACTTGAACAGGATTTGTTCTACAGGTTGTACAGGTTGTACTACTGTGTCCTCAGTTCTAAAATTCTTTTGATACTTAGCTATACTCTGTTTTATCATGTGCAGGTTGTAGGCAAAAAGTACATTAGGCTATATGATGCTTCATTGTCTGAGGAACTTTACCCATACACTGAAACCATGCTTTGCAATTCCAGCCAGGTAACCGCTGATAATTCTTTCTATGTATACAATGGCCAATATAAAAAATTGATCATCAATTCGATGAAAAACCGACATGATTATGTTTTAAAATTCTGGAACATTACAAGGAGATTTATGTTTCTTCATTAGTTATCATCTCATATCATTGATTCTTAAATTTGTTTTGACGATAATCTGCTGAGAGTTATTCCTTCCACTGTTTTTGATTTTACTCTTTACAAGCTTACATATTTCTTTTTTTGTGTATAAATCTTACTTTGAAGGTCGATCTGGACAACATAGATGAGAAAGAATTTCCAAAAGTGGTGGACTTGGAATTTGTGGACTGCATTCTAGAGGAAGGTGAGATGCTTTATATCCCACCAAAATGGTGGCACTATGTTCGATCCTTAACAACTAGCTTTTCTGTTAGCTTTTGGTGGAATAATTGTGATGAAAATTCAACCAGTTCCTGA

mRNA sequence

ATGTCTTCATCGGAACCCAACCAAAACCCTTCTTCATACCATTTCGAAACCCCTCTTCTCGACGCCGAGTCGCCGTCGCTGCTCCACTCCATCTCCGAGCACGGTGGCTACGCCTACGTCAGCATGGCGGCGCTCGCGGCTTCCGGGGACTCATGCGCTGCCGAGGCCGCCGGCGAGATGGCCTGGGAGCAGCTTCACTCTGGCCCCTGGCACTCTGTTCTCCCTATCTGGCGCGATGCCTACTCCATGGCTTGCCTCTACATGGCGAGGTTCCATTTTGCTGGCGGCGAGTTTAAGGAGGCTCTTAGGGTCTTGGATATGGGGCTGATCATGGGCGGCCCGCTTTTGAGGAGTGATTTGGATTCTGCCATTGCGAAGGTCTCTGCTGAGGCGAGGATTGTTAGGGTTTTGGATGTTAACCAGAAGGATGCCCAGTTGGAGCCTCCGTTGGGTTGTGAGGAGCGTAATAAGGAGGTTCCTTGGAATTTGCCTGTAAAGGCTCTCAGTAATAAGATGGTTGCAAAGATGTCAGCTTTATCTCTAGAGGGATTCTTGCGTGAATATTTCCAACCGGGTTTCCCAGTTATTATTAGTGATGGTATGGCTCATTGGCCAGCTAGGACAAAGTGGAAGAGCATGGATTACTTACAAAAGGTTGCTGGTGACCGTACAATCCCAGTTGAGGTTGGGAAGAACTATTTACGCCCAGAATGGAAACAAGAGCTTATCACATTTTCCGAATTTCTCGGCAGGATTCAGTTGAATGACTGTTCTGATGATATTACATATCTTGCTCAACATCCATTATTTGACCAGATAGATGAGCTCCAGAAGGACATTTGTATTCCTGATTACTGTTTTGTTGGTGGCGGAGAGTTAAGATCTCTTAATGCTTGGTTTGGCCCACCTGGGACAGTAACACCTTTACACCATGATCCTCATCACAATATATTAGCTCAGGTTGTAGGCAAAAAGTACATTAGGCTATATGATGCTTCATTGTCTGAGGAACTTTACCCATACACTGAAACCATGCTTTGCAATTCCAGCCAGGTCGATCTGGACAACATAGATGAGAAAGAATTTCCAAAAGTGGTGGACTTGGAATTTGTGGACTGCATTCTAGAGGAAGGTGAGATGCTTTATATCCCACCAAAATGGTGGCACTATGTTCGATCCTTAACAACTAGCTTTTCTGTTAGCTTTTGGTGGAATAATTGTGATGAAAATTCAACCAGTTCCTGA

Coding sequence (CDS)

ATGTCTTCATCGGAACCCAACCAAAACCCTTCTTCATACCATTTCGAAACCCCTCTTCTCGACGCCGAGTCGCCGTCGCTGCTCCACTCCATCTCCGAGCACGGTGGCTACGCCTACGTCAGCATGGCGGCGCTCGCGGCTTCCGGGGACTCATGCGCTGCCGAGGCCGCCGGCGAGATGGCCTGGGAGCAGCTTCACTCTGGCCCCTGGCACTCTGTTCTCCCTATCTGGCGCGATGCCTACTCCATGGCTTGCCTCTACATGGCGAGGTTCCATTTTGCTGGCGGCGAGTTTAAGGAGGCTCTTAGGGTCTTGGATATGGGGCTGATCATGGGCGGCCCGCTTTTGAGGAGTGATTTGGATTCTGCCATTGCGAAGGTCTCTGCTGAGGCGAGGATTGTTAGGGTTTTGGATGTTAACCAGAAGGATGCCCAGTTGGAGCCTCCGTTGGGTTGTGAGGAGCGTAATAAGGAGGTTCCTTGGAATTTGCCTGTAAAGGCTCTCAGTAATAAGATGGTTGCAAAGATGTCAGCTTTATCTCTAGAGGGATTCTTGCGTGAATATTTCCAACCGGGTTTCCCAGTTATTATTAGTGATGGTATGGCTCATTGGCCAGCTAGGACAAAGTGGAAGAGCATGGATTACTTACAAAAGGTTGCTGGTGACCGTACAATCCCAGTTGAGGTTGGGAAGAACTATTTACGCCCAGAATGGAAACAAGAGCTTATCACATTTTCCGAATTTCTCGGCAGGATTCAGTTGAATGACTGTTCTGATGATATTACATATCTTGCTCAACATCCATTATTTGACCAGATAGATGAGCTCCAGAAGGACATTTGTATTCCTGATTACTGTTTTGTTGGTGGCGGAGAGTTAAGATCTCTTAATGCTTGGTTTGGCCCACCTGGGACAGTAACACCTTTACACCATGATCCTCATCACAATATATTAGCTCAGGTTGTAGGCAAAAAGTACATTAGGCTATATGATGCTTCATTGTCTGAGGAACTTTACCCATACACTGAAACCATGCTTTGCAATTCCAGCCAGGTCGATCTGGACAACATAGATGAGAAAGAATTTCCAAAAGTGGTGGACTTGGAATTTGTGGACTGCATTCTAGAGGAAGGTGAGATGCTTTATATCCCACCAAAATGGTGGCACTATGTTCGATCCTTAACAACTAGCTTTTCTGTTAGCTTTTGGTGGAATAATTGTGATGAAAATTCAACCAGTTCCTGA

Protein sequence

MSSSEPNQNPSSYHFETPLLDAESPSLLHSISEHGGYAYVSMAALAASGDSCAAEAAGEMAWEQLHSGPWHSVLPIWRDAYSMACLYMARFHFAGGEFKEALRVLDMGLIMGGPLLRSDLDSAIAKVSAEARIVRVLDVNQKDAQLEPPLGCEERNKEVPWNLPVKALSNKMVAKMSALSLEGFLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQELITFSEFLGRIQLNDCSDDITYLAQHPLFDQIDELQKDICIPDYCFVGGGELRSLNAWFGPPGTVTPLHHDPHHNILAQVVGKKYIRLYDASLSEELYPYTETMLCNSSQVDLDNIDEKEFPKVVDLEFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDENSTSS
Homology
BLAST of Lag0034859 vs. NCBI nr
Match: XP_022967535.1 (lysine-specific demethylase JMJ30-like [Cucurbita maxima])

HSP 1 Score: 796.2 bits (2055), Expect = 1.4e-226
Identity = 379/410 (92.44%), Postives = 393/410 (95.85%), Query Frame = 0

Query: 1   MSSSEPNQNPSSYHFETPLLDAESPSLLHSISEHGGYAYVSMAALAASGDSCAAEAAGEM 60
           MSSS     PSSYHFETPLLDAE PSLLHSI EHGGYAY+SMAALAA+GDS AAEAA EM
Sbjct: 1   MSSSGAKDGPSSYHFETPLLDAELPSLLHSICEHGGYAYISMAALAAAGDSHAAEAASEM 60

Query: 61  AWEQLHSGPWHSVLPIWRDAYSMACLYMARFHFAGGEFKEALRVLDMGLIMGGPLLRSDL 120
           AWEQLHSGPWHSVLPIWRDAYSMACLYMARFHFAGG+FKEALRVLDMG+IMGGPLLRSDL
Sbjct: 61  AWEQLHSGPWHSVLPIWRDAYSMACLYMARFHFAGGDFKEALRVLDMGVIMGGPLLRSDL 120

Query: 121 DSAIAKVSAEARIVRVLDVNQKDAQLEPPLGCEERNKEVPWNLPVKALSNKMVAKMSALS 180
           DSAIAKVSA AR+VRVLDV +KD QLEPPLGCEERN EVPWNLPVKAL+NK+VAKMSALS
Sbjct: 121 DSAIAKVSAAARVVRVLDVAEKDDQLEPPLGCEERNNEVPWNLPVKALTNKIVAKMSALS 180

Query: 181 LEGFLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQ 240
           LEGFLREYFQPGFPVIISDGMAHWPARTKWK+MDYLQKVAGDRTIPVEVGKNYLRPEWKQ
Sbjct: 181 LEGFLREYFQPGFPVIISDGMAHWPARTKWKNMDYLQKVAGDRTIPVEVGKNYLRPEWKQ 240

Query: 241 ELITFSEFLGRIQLNDCSDDITYLAQHPLFDQIDELQKDICIPDYCFVGGGELRSLNAWF 300
           ELITFSEFL RIQ N+ SDDITYLAQHPLFDQIDEL+KDICIPDYCFVGGGELRSLNAWF
Sbjct: 241 ELITFSEFLVRIQSNNRSDDITYLAQHPLFDQIDELRKDICIPDYCFVGGGELRSLNAWF 300

Query: 301 GPPGTVTPLHHDPHHNILAQVVGKKYIRLYDASLSEELYPYTETMLCNSSQVDLDNIDEK 360
           GPPGTVTPLHHDPHHNILAQV+GKKY+RLYDASL EELYPYTETMLCNSSQVDLDNIDEK
Sbjct: 301 GPPGTVTPLHHDPHHNILAQVLGKKYVRLYDASLCEELYPYTETMLCNSSQVDLDNIDEK 360

Query: 361 EFPKVVDLEFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDEN 411
           EFPKVVDL+FVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDEN
Sbjct: 361 EFPKVVDLDFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDEN 410

BLAST of Lag0034859 vs. NCBI nr
Match: XP_022927851.1 (lysine-specific demethylase JMJ30-like [Cucurbita moschata])

HSP 1 Score: 792.3 bits (2045), Expect = 2.0e-225
Identity = 380/410 (92.68%), Postives = 392/410 (95.61%), Query Frame = 0

Query: 1   MSSSEPNQNPSSYHFETPLLDAESPSLLHSISEHGGYAYVSMAALAASGDSCAAEAAGEM 60
           MSSS     PSSYHFETPLLDAE PSLLHSI EHGGYAY+SMAALAA+GDS AAEAA EM
Sbjct: 1   MSSSGAKDGPSSYHFETPLLDAELPSLLHSICEHGGYAYISMAALAAAGDSHAAEAASEM 60

Query: 61  AWEQLHSGPWHSVLPIWRDAYSMACLYMARFHFAGGEFKEALRVLDMGLIMGGPLLRSDL 120
           AWEQLHSGPWHSVLPIWRDAYSMACLY+ARFHFAGG+FKEALRVLDMG+IMGGPLLRSDL
Sbjct: 61  AWEQLHSGPWHSVLPIWRDAYSMACLYLARFHFAGGDFKEALRVLDMGVIMGGPLLRSDL 120

Query: 121 DSAIAKVSAEARIVRVLDVNQKDAQLEPPLGCEERNKEVPWNLPVKALSNKMVAKMSALS 180
           DSAIAKVSA AR+VRVLDV +KD QLEPPLGCEERN EVP NLPVKAL+NK+VAKMSALS
Sbjct: 121 DSAIAKVSAAARVVRVLDVAEKDDQLEPPLGCEERNNEVPSNLPVKALTNKIVAKMSALS 180

Query: 181 LEGFLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQ 240
           LEGFLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQ
Sbjct: 181 LEGFLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQ 240

Query: 241 ELITFSEFLGRIQLNDCSDDITYLAQHPLFDQIDELQKDICIPDYCFVGGGELRSLNAWF 300
           ELITFSEFL RIQ N+ SDDITYLAQHPLFDQIDEL+KDICIPDYCFVGGGELRSLNAWF
Sbjct: 241 ELITFSEFLVRIQSNNRSDDITYLAQHPLFDQIDELRKDICIPDYCFVGGGELRSLNAWF 300

Query: 301 GPPGTVTPLHHDPHHNILAQVVGKKYIRLYDASLSEELYPYTETMLCNSSQVDLDNIDEK 360
           GPPGTVTPLHHDPHHNILAQVVGKKYIRLYDASL EELYPYTETMLCNSSQVDLDNIDEK
Sbjct: 301 GPPGTVTPLHHDPHHNILAQVVGKKYIRLYDASLCEELYPYTETMLCNSSQVDLDNIDEK 360

Query: 361 EFPKVVDLEFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDEN 411
           EFPKVVDL+FVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDEN
Sbjct: 361 EFPKVVDLDFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDEN 410

BLAST of Lag0034859 vs. NCBI nr
Match: KAG6588179.1 (Lysine-specific demethylase JMJ30, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022075.1 Lysine-specific demethylase JMJ30 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 788.1 bits (2034), Expect = 3.7e-224
Identity = 378/410 (92.20%), Postives = 390/410 (95.12%), Query Frame = 0

Query: 1   MSSSEPNQNPSSYHFETPLLDAESPSLLHSISEHGGYAYVSMAALAASGDSCAAEAAGEM 60
           MSSS     PSSYHFETPLLDAE PSLLHSI EHGGYAY+SMA LAA+GDS AA AA EM
Sbjct: 1   MSSSGAKDGPSSYHFETPLLDAELPSLLHSICEHGGYAYISMATLAAAGDSHAAGAASEM 60

Query: 61  AWEQLHSGPWHSVLPIWRDAYSMACLYMARFHFAGGEFKEALRVLDMGLIMGGPLLRSDL 120
           AWEQLHSGPWHSVLPIWRDAYSMACLY+ARFHFAGG+FKEALRVLDMG+IMGGPLLRSDL
Sbjct: 61  AWEQLHSGPWHSVLPIWRDAYSMACLYLARFHFAGGDFKEALRVLDMGVIMGGPLLRSDL 120

Query: 121 DSAIAKVSAEARIVRVLDVNQKDAQLEPPLGCEERNKEVPWNLPVKALSNKMVAKMSALS 180
           DSAIAKVSA AR+VRVLDV +KD QLEPPLGCEERN EVP NLPVKAL+NK+VAKMSALS
Sbjct: 121 DSAIAKVSAAARVVRVLDVAEKDDQLEPPLGCEERNNEVPSNLPVKALTNKIVAKMSALS 180

Query: 181 LEGFLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQ 240
           LEGFLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQ
Sbjct: 181 LEGFLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQ 240

Query: 241 ELITFSEFLGRIQLNDCSDDITYLAQHPLFDQIDELQKDICIPDYCFVGGGELRSLNAWF 300
           ELITFSEFL RIQ N+ SDDITYLAQHPLFDQIDEL+KDICIPDYCFVGGGELRSLNAWF
Sbjct: 241 ELITFSEFLVRIQSNNRSDDITYLAQHPLFDQIDELRKDICIPDYCFVGGGELRSLNAWF 300

Query: 301 GPPGTVTPLHHDPHHNILAQVVGKKYIRLYDASLSEELYPYTETMLCNSSQVDLDNIDEK 360
           GPPGTVTPLHHDPHHNILAQVVGKKYIRLYDASL EELYPYTETMLCNSSQVDLDNIDEK
Sbjct: 301 GPPGTVTPLHHDPHHNILAQVVGKKYIRLYDASLCEELYPYTETMLCNSSQVDLDNIDEK 360

Query: 361 EFPKVVDLEFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDEN 411
           EFPKVVDL+FVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDEN
Sbjct: 361 EFPKVVDLDFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDEN 410

BLAST of Lag0034859 vs. NCBI nr
Match: XP_023529860.1 (lysine-specific demethylase JMJ30-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 788.1 bits (2034), Expect = 3.7e-224
Identity = 377/410 (91.95%), Postives = 389/410 (94.88%), Query Frame = 0

Query: 1   MSSSEPNQNPSSYHFETPLLDAESPSLLHSISEHGGYAYVSMAALAASGDSCAAEAAGEM 60
           MSSS     PSSYHFETPLLDAE PSLLHSI EHGGYAY+SMA LAA+GDS AAEAA EM
Sbjct: 1   MSSSGAKDGPSSYHFETPLLDAELPSLLHSICEHGGYAYISMATLAAAGDSHAAEAASEM 60

Query: 61  AWEQLHSGPWHSVLPIWRDAYSMACLYMARFHFAGGEFKEALRVLDMGLIMGGPLLRSDL 120
           AWEQLHSGPWHSVLPIWRDAYSMACLY+ARFHFAGG+FKEALRVLDMG+IMGGPLLRSDL
Sbjct: 61  AWEQLHSGPWHSVLPIWRDAYSMACLYLARFHFAGGDFKEALRVLDMGVIMGGPLLRSDL 120

Query: 121 DSAIAKVSAEARIVRVLDVNQKDAQLEPPLGCEERNKEVPWNLPVKALSNKMVAKMSALS 180
           DSAIAKVSA AR+VRVLDV +KD QLEPPLGCEERN EV WNLPVKAL+NK+VAK SALS
Sbjct: 121 DSAIAKVSAAARVVRVLDVAEKDDQLEPPLGCEERNNEVSWNLPVKALTNKIVAKTSALS 180

Query: 181 LEGFLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQ 240
           LEGFLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAG RTIPVEVGKNYLRPEWKQ
Sbjct: 181 LEGFLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGGRTIPVEVGKNYLRPEWKQ 240

Query: 241 ELITFSEFLGRIQLNDCSDDITYLAQHPLFDQIDELQKDICIPDYCFVGGGELRSLNAWF 300
           ELITFSEFL RIQ N+ SDDITYLAQHPLFDQIDEL+KDICIPDYCFVGGGELRSLNAWF
Sbjct: 241 ELITFSEFLVRIQSNNRSDDITYLAQHPLFDQIDELRKDICIPDYCFVGGGELRSLNAWF 300

Query: 301 GPPGTVTPLHHDPHHNILAQVVGKKYIRLYDASLSEELYPYTETMLCNSSQVDLDNIDEK 360
           GPPGTVTPLHHDPHHNILAQVVGKKYIRLYDASL EELYPYTETMLCNSSQVDLDNIDEK
Sbjct: 301 GPPGTVTPLHHDPHHNILAQVVGKKYIRLYDASLCEELYPYTETMLCNSSQVDLDNIDEK 360

Query: 361 EFPKVVDLEFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDEN 411
           EFPKVVDL+FVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDEN
Sbjct: 361 EFPKVVDLDFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDEN 410

BLAST of Lag0034859 vs. NCBI nr
Match: XP_038878408.1 (lysine-specific demethylase JMJ30 [Benincasa hispida])

HSP 1 Score: 785.4 bits (2027), Expect = 2.4e-223
Identity = 378/414 (91.30%), Postives = 395/414 (95.41%), Query Frame = 0

Query: 1   MSSSEPNQNPSSYHFETPLLDAESPSLLHSISEHGGYAYVSMAALAASGDSCAAEAAGEM 60
           M+SSE  Q+PSS+HFETPLLDAES SLLHSISEHGGYAYVSMAALAA+GD  AAEAAGEM
Sbjct: 1   MASSESKQSPSSFHFETPLLDAESHSLLHSISEHGGYAYVSMAALAAAGDIRAAEAAGEM 60

Query: 61  AWEQLHSGPWHSVLPIWRDAYSMACLYMARFHFAGGEFKEALRVLDMGLIMGGPLLRSDL 120
           AWEQLHSGPWHSVLPIWRDAYSMACLYMARFHFAGGEFKEALRVLDMGLIMGGPLLR DL
Sbjct: 61  AWEQLHSGPWHSVLPIWRDAYSMACLYMARFHFAGGEFKEALRVLDMGLIMGGPLLRDDL 120

Query: 121 DSAIAKVSAEARIVRVLDVNQKDAQLEPPLGCEERNKEVPWNLPVKALSNKMVAKMSALS 180
           DSAIAKVSAEARIVRVL+    D Q+E  LGCEERNKEV WNLPVKAL+NKM+AKMSALS
Sbjct: 121 DSAIAKVSAEARIVRVLE----DGQMESSLGCEERNKEVAWNLPVKALTNKMIAKMSALS 180

Query: 181 LEGFLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQ 240
           LEGFLREYF+PGFPVII+DGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQ
Sbjct: 181 LEGFLREYFRPGFPVIINDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQ 240

Query: 241 ELITFSEFLGRIQLNDCSDDITYLAQHPLFDQIDELQKDICIPDYCFVGGGELRSLNAWF 300
           ELITFSEFL RIQ ND SDDITYLAQHPLFDQI+EL+KDICIPDYC +GGGELRSLNAWF
Sbjct: 241 ELITFSEFLSRIQSNDRSDDITYLAQHPLFDQINELRKDICIPDYCSIGGGELRSLNAWF 300

Query: 301 GPPGTVTPLHHDPHHNILAQVVGKKYIRLYDASLSEELYPYTETMLCNSSQVDLDNIDEK 360
           GPPGTVTPLHHDPHHNILAQV+GKKYIRLYDASLSEELYPY ETMLCNSSQVDLD+I+EK
Sbjct: 301 GPPGTVTPLHHDPHHNILAQVIGKKYIRLYDASLSEELYPYNETMLCNSSQVDLDDINEK 360

Query: 361 EFPKVVDLEFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDENSTSS 415
           EFPKV+DLEFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDENSTSS
Sbjct: 361 EFPKVMDLEFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDENSTSS 410

BLAST of Lag0034859 vs. ExPASy Swiss-Prot
Match: Q8RWR1 (Lysine-specific demethylase JMJ30 OS=Arabidopsis thaliana OX=3702 GN=JMJ30 PE=1 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 2.5e-159
Identity = 279/411 (67.88%), Postives = 326/411 (79.32%), Query Frame = 0

Query: 17  TPLLDAESPSLLHSISEHGGYAYVSMAALAASGDSCAAEAAGEMAWEQLHSGPWHSVLPI 76
           TP LDAES +LL SIS  GGYAY  MA LA +GD  AAEAA +MAWEQLHSGPWHSVLP+
Sbjct: 19  TPTLDAESQTLLQSISAEGGYAYARMAVLAVAGDQSAAEAARDMAWEQLHSGPWHSVLPV 78

Query: 77  WRDAYSMACLYMARFHFAGGEFKEALRVLDMGLIMGGPLLRSDLDSAIAKVSAEAR-IVR 136
           WRDAYSMACL++A+ HFA GEF EAL  LDMGLIMGG LLR DL  ++  VS+EAR + +
Sbjct: 79  WRDAYSMACLHVAKIHFAAGEFGEALGALDMGLIMGGMLLRKDLHDSVLLVSSEARKMTK 138

Query: 137 VLDV---NQKDAQLEPPLGCEERN-KEVPWN--------LPVKALSNKMVAKMSALSLEG 196
            L+    + K  +L P +  +    + V  N        LP ++L+ K V K S LSLEG
Sbjct: 139 SLEEASGDFKGERLVPEVPVDVNEVRHVLANLQLLVLKILPCRSLTCKRVEKRSGLSLEG 198

Query: 197 FLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQELI 256
           FLR+Y+ PG PV+I++ MAHWPARTKW  +DYL  VAG+RT+PVEVGKNYL  +WKQEL+
Sbjct: 199 FLRDYYLPGTPVVITNSMAHWPARTKWNHLDYLNAVAGNRTVPVEVGKNYLCSDWKQELV 258

Query: 257 TFSEFLGRIQLNDCSD-DITYLAQHPLFDQIDELQKDICIPDYCFVGGGELRSLNAWFGP 316
           TFS+FL R++ N  S  + TYLAQHPLFDQI+EL+ DICIPDYCFVGGGEL+SLNAWFGP
Sbjct: 259 TFSKFLERMRTNKSSPMEPTYLAQHPLFDQINELRDDICIPDYCFVGGGELQSLNAWFGP 318

Query: 317 PGTVTPLHHDPHHNILAQVVGKKYIRLYDASLSEELYPYTETMLCNSSQVDLDNIDEKEF 376
            GTVTPLHHDPHHNILAQVVGKKYIRLY + L +ELYPY+ETMLCNSSQVDLDNIDE EF
Sbjct: 319 AGTVTPLHHDPHHNILAQVVGKKYIRLYPSFLQDELYPYSETMLCNSSQVDLDNIDETEF 378

Query: 377 PKVVDLEFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDENSTS 414
           PK ++LEF+DCILEEGEMLYIPPKWWHYVRSLT S SVSFWW+N  E+S+S
Sbjct: 379 PKAMELEFMDCILEEGEMLYIPPKWWHYVRSLTMSLSVSFWWSNEAESSSS 429

BLAST of Lag0034859 vs. ExPASy Swiss-Prot
Match: Q9CXT6 (Bifunctional peptidase and arginyl-hydroxylase JMJD5 OS=Mus musculus OX=10090 GN=Kdm8 PE=1 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 8.9e-80
Identity = 156/364 (42.86%), Postives = 218/364 (59.89%), Query Frame = 0

Query: 53  AAEAAGEMAWEQLHSGPWHSVLPIWRDAYSMACLYMA----RFHFAGGEFKEALRVLDMG 112
           A+EA  + +WE+L++GPW  V   WR  YS  CL  A    +         EALRV DMG
Sbjct: 64  ASEAVLDYSWEKLNTGPWRDVDKEWRRVYSFGCLLKALCLCQAPQKATTVVEALRVCDMG 123

Query: 113 LIMGGPLLRSDLDSAIAKVSAEARIVRVLDVNQKDAQLEPPLGCEE-----RNKEVPWNL 172
           L+MG  +L   L           ++V VL  +Q   + +P  G  +     +  +   + 
Sbjct: 124 LLMGAAILEDIL----------LKVVAVLQTHQLPGK-QPARGPHQDQPATKKAKCDASP 183

Query: 173 PVKALSNKMVAKMSALSLEGFLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGDR 232
               +  +MV ++    L+ F + +  PG PVI+     HWP   KW S+ Y+Q++AG R
Sbjct: 184 APDVMLERMVPRLRCPPLQYFKQHFLVPGRPVILEGVADHWPCMKKW-SLQYIQEIAGCR 243

Query: 233 TIPVEVGKNYLRPEWKQELITFSEFLGRIQLNDCSDDITYLAQHPLFDQIDELQKDICIP 292
           T+PVEVG  Y   +W Q L+T  EF+ +  L++ + D+ YLAQH LFDQI EL++DI IP
Sbjct: 244 TVPVEVGSRYTDEDWSQTLMTVDEFIQKFILSE-AKDVGYLAQHQLFDQIPELKRDISIP 303

Query: 293 DYCFVGGGELR--SLNAWFGPPGTVTPLHHDPHHNILAQVVGKKYIRLYDASLSEELYPY 352
           DYC +G GE    ++NAWFGP GT++PLH DP  N L QV+G+KYIRLY    SE +YP+
Sbjct: 304 DYCCLGNGEEEEITINAWFGPQGTISPLHQDPQQNFLVQVLGRKYIRLYSPQESEAVYPH 363

Query: 353 TETMLCNSSQVDLDNIDEKEFPKVVDLEFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVS 406
              +L N+SQVD++N D ++FPK  +  F+ CIL  G+ L+IP K+WHYVRSL  SFSVS
Sbjct: 364 ETHILHNTSQVDVENPDLEKFPKFTEAPFLSCILSPGDTLFIPAKYWHYVRSLDLSFSVS 414

BLAST of Lag0034859 vs. ExPASy Swiss-Prot
Match: Q1JP61 (Bifunctional peptidase and arginyl-hydroxylase JMJD5 OS=Bos taurus OX=9913 GN=KDM8 PE=2 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 9.8e-79
Identity = 157/356 (44.10%), Postives = 215/356 (60.39%), Query Frame = 0

Query: 59  EMAWEQLHSGPWHSVLPIWRDAYSMACLYMA----RFHFAGGEFKEALRVLDMGLIMGGP 118
           + +WE+L++GPW  V   WR  Y+  CL  A               AL+  DMGL+MG  
Sbjct: 62  DYSWEKLNTGPWQHVDKDWRRVYAFGCLLKAVCLCEPPGDAASVAAALKACDMGLLMGAA 121

Query: 119 LLRSDLDSAIAKVSAEARIVRVLDVNQKDA---QLEPPLGCEERNKEVPWNLPVKALSNK 178
           +L   L      +   A + + L   ++ A     EPP   + RN  VP  +P    + +
Sbjct: 122 ILGDIL------LKVAAVLQKYLLSGKRPAPGPSQEPPGTKKARNDHVP--IP-DVTTER 181

Query: 179 MVAKMSALSLEGFLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGK 238
            V ++   SL+ F + +  PG PVI+     HWP   KW S++Y+Q+VAG RT+PVEVG 
Sbjct: 182 TVPRLHCPSLQYFKKHFLVPGRPVILEGVANHWPCMKKW-SLEYIQEVAGCRTVPVEVGS 241

Query: 239 NYLRPEWKQELITFSEFLGRIQLNDCSDDITYLAQHPLFDQIDELQKDICIPDYCFVGGG 298
            Y   EW Q L+T +EF+ +  + +   DI YLAQH LFDQI EL++DI IPDYC +G G
Sbjct: 242 RYTDEEWSQTLMTVNEFISK-YIREEPKDIGYLAQHQLFDQIPELKQDISIPDYCCLGDG 301

Query: 299 ELR--SLNAWFGPPGTVTPLHHDPHHNILAQVVGKKYIRLYDASLSEELYPYTETMLCNS 358
           E    ++NAWFGP GTV+PLH DP  N LAQV+G+KYIRLY    SE LYP+   +L N+
Sbjct: 302 EEEEITINAWFGPQGTVSPLHQDPQQNFLAQVMGRKYIRLYSPQESEALYPHDTHLLHNT 361

Query: 359 SQVDLDNIDEKEFPKVVDLEFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWN 406
           SQVD++N D ++FP+  +  F+ C+L  GE+L+IP K WHYVR+L  SFSVSFWW+
Sbjct: 362 SQVDVENPDLEKFPRFAEAPFLSCVLSPGEVLFIPVKHWHYVRALDLSFSVSFWWS 406

BLAST of Lag0034859 vs. ExPASy Swiss-Prot
Match: Q497B8 (Bifunctional peptidase and arginyl-hydroxylase JMJD5 OS=Rattus norvegicus OX=10116 GN=Kdm8 PE=2 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 2.9e-78
Identity = 155/359 (43.18%), Postives = 216/359 (60.17%), Query Frame = 0

Query: 53  AAEAAGEMAWEQLHSGPWHSVLPIWRDAYSMACLY----MARFHFAGGEFKEALRVLDMG 112
           A+EA  + +WE+L++GPW  V   WR  YS  CL     + +         EALRV DMG
Sbjct: 64  ASEAVLDYSWEKLNTGPWRDVDKEWRRVYSFGCLLKTLCLCQAPQKATAVAEALRVCDMG 123

Query: 113 LIMGGPLLRSDLDSAIAKVSAEARIVRVLDVNQKDAQLEPPLGCEERNKEVPWNLPVKAL 172
           L+MG  +L  D+   +A V        +L   Q            ++ K    + P   L
Sbjct: 124 LLMGAAIL-GDILLKVATVLQ----THLLPRKQPACGPHQDQPATKKAKHDASSTPDVVL 183

Query: 173 SNKMVAKMSALSLEGFLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVE 232
            ++ V ++    L+ F + +  PG PVI+     HWP   KW S+ Y+Q++AG RT+PVE
Sbjct: 184 -DREVPRLRCPPLQHFKKHFLVPGRPVILEGVADHWPCMKKW-SLQYIQEIAGCRTVPVE 243

Query: 233 VGKNYLRPEWKQELITFSEFLGRIQLNDCSDDITYLAQHPLFDQIDELQKDICIPDYCFV 292
           VG  Y   +W Q L+T +EF+ +  L++ + D+ YLAQH LFDQI EL++DI IPDYC +
Sbjct: 244 VGSRYTDEDWSQTLMTVNEFIHKYILSE-AKDVGYLAQHQLFDQIPELKQDISIPDYCCL 303

Query: 293 GGGELR--SLNAWFGPPGTVTPLHHDPHHNILAQVVGKKYIRLYDASLSEELYPYTETML 352
           G GE    ++NAWFGP GT++PLH DP  N L QV+G+KYIRLY    SE +YP+   +L
Sbjct: 304 GNGEEEEITINAWFGPQGTISPLHQDPQQNFLVQVLGRKYIRLYSPQESEAVYPHETHIL 363

Query: 353 CNSSQVDLDNIDEKEFPKVVDLEFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWN 406
            N+SQVD++N D ++FPK  +  F+ CIL  G+ L+IP K+WHYVRSL  SFSVSFWW+
Sbjct: 364 HNTSQVDVENPDLEKFPKFTEAPFLSCILSPGDTLFIPAKYWHYVRSLDLSFSVSFWWS 414

BLAST of Lag0034859 vs. ExPASy Swiss-Prot
Match: Q8N371 (Bifunctional peptidase and arginyl-hydroxylase JMJD5 OS=Homo sapiens OX=9606 GN=KDM8 PE=1 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 2.4e-77
Identity = 158/365 (43.29%), Postives = 221/365 (60.55%), Query Frame = 0

Query: 50  DSC--AAEAAGEMAWEQLHSGPWHSVLPIWRDAYSMACLYMARFHFAGGE----FKEALR 109
           D C  ++E   + +WE+L++G W  V   WR  Y++ CL  A       E       ALR
Sbjct: 62  DECLQSSEVILDYSWEKLNTGTWQDVDKDWRRVYAIGCLLKALCLCQAPEDANTVAAALR 121

Query: 110 VLDMGLIMGGPLLRSDLDSAIAKVSAEARIVRVLDVNQKDAQLE-PPLGCEERNKEVPWN 169
           V DMGL+MG  +    L   + KV+A   I++     ++ A+   P   C ++ +     
Sbjct: 122 VCDMGLLMGAAI----LGDILLKVAA---ILQTHLPGKRPARGSLPEQPCTKKARADHGL 181

Query: 170 LPVKALSNKMVAKMSALSLEGFLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGD 229
           +P   L  K V ++   SL+ F  ++  PG PVI+     HWP   KW S++Y+Q++AG 
Sbjct: 182 IPDVKL-EKTVPRLHRPSLQHFREQFLVPGRPVILKGVADHWPCMQKW-SLEYIQEIAGC 241

Query: 230 RTIPVEVGKNYLRPEWKQELITFSEFLGRIQLNDCSDDITYLAQHPLFDQIDELQKDICI 289
           RT+PVEVG  Y   EW Q L+T +EF+ +  +N+   D+ YLAQH LFDQI EL++DI I
Sbjct: 242 RTVPVEVGSRYTDEEWSQTLMTVNEFISKYIVNE-PRDVGYLAQHQLFDQIPELKQDISI 301

Query: 290 PDYCFVGGGELR--SLNAWFGPPGTVTPLHHDPHHNILAQVVGKKYIRLYDASLSEELYP 349
           PDYC +G GE    ++NAWFGP GT++PLH DP  N L QV+G+KYIRLY    S  LYP
Sbjct: 302 PDYCSLGDGEEEEITINAWFGPQGTISPLHQDPQQNFLVQVMGRKYIRLYSPQESGALYP 361

Query: 350 YTETMLCNSSQVDLDNIDEKEFPKVVDLEFVDCILEEGEMLYIPPKWWHYVRSLTTSFSV 406
           +   +L N+SQVD++N D ++FPK     F+ CIL  GE+L+IP K+WHYVR+L  SFSV
Sbjct: 362 HDTHLLHNTSQVDVENPDLEKFPKFAKAPFLSCILSPGEILFIPVKYWHYVRALDLSFSV 416

BLAST of Lag0034859 vs. ExPASy TrEMBL
Match: A0A6J1HUR9 (lysine-specific demethylase JMJ30-like OS=Cucurbita maxima OX=3661 GN=LOC111467010 PE=4 SV=1)

HSP 1 Score: 796.2 bits (2055), Expect = 6.5e-227
Identity = 379/410 (92.44%), Postives = 393/410 (95.85%), Query Frame = 0

Query: 1   MSSSEPNQNPSSYHFETPLLDAESPSLLHSISEHGGYAYVSMAALAASGDSCAAEAAGEM 60
           MSSS     PSSYHFETPLLDAE PSLLHSI EHGGYAY+SMAALAA+GDS AAEAA EM
Sbjct: 1   MSSSGAKDGPSSYHFETPLLDAELPSLLHSICEHGGYAYISMAALAAAGDSHAAEAASEM 60

Query: 61  AWEQLHSGPWHSVLPIWRDAYSMACLYMARFHFAGGEFKEALRVLDMGLIMGGPLLRSDL 120
           AWEQLHSGPWHSVLPIWRDAYSMACLYMARFHFAGG+FKEALRVLDMG+IMGGPLLRSDL
Sbjct: 61  AWEQLHSGPWHSVLPIWRDAYSMACLYMARFHFAGGDFKEALRVLDMGVIMGGPLLRSDL 120

Query: 121 DSAIAKVSAEARIVRVLDVNQKDAQLEPPLGCEERNKEVPWNLPVKALSNKMVAKMSALS 180
           DSAIAKVSA AR+VRVLDV +KD QLEPPLGCEERN EVPWNLPVKAL+NK+VAKMSALS
Sbjct: 121 DSAIAKVSAAARVVRVLDVAEKDDQLEPPLGCEERNNEVPWNLPVKALTNKIVAKMSALS 180

Query: 181 LEGFLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQ 240
           LEGFLREYFQPGFPVIISDGMAHWPARTKWK+MDYLQKVAGDRTIPVEVGKNYLRPEWKQ
Sbjct: 181 LEGFLREYFQPGFPVIISDGMAHWPARTKWKNMDYLQKVAGDRTIPVEVGKNYLRPEWKQ 240

Query: 241 ELITFSEFLGRIQLNDCSDDITYLAQHPLFDQIDELQKDICIPDYCFVGGGELRSLNAWF 300
           ELITFSEFL RIQ N+ SDDITYLAQHPLFDQIDEL+KDICIPDYCFVGGGELRSLNAWF
Sbjct: 241 ELITFSEFLVRIQSNNRSDDITYLAQHPLFDQIDELRKDICIPDYCFVGGGELRSLNAWF 300

Query: 301 GPPGTVTPLHHDPHHNILAQVVGKKYIRLYDASLSEELYPYTETMLCNSSQVDLDNIDEK 360
           GPPGTVTPLHHDPHHNILAQV+GKKY+RLYDASL EELYPYTETMLCNSSQVDLDNIDEK
Sbjct: 301 GPPGTVTPLHHDPHHNILAQVLGKKYVRLYDASLCEELYPYTETMLCNSSQVDLDNIDEK 360

Query: 361 EFPKVVDLEFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDEN 411
           EFPKVVDL+FVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDEN
Sbjct: 361 EFPKVVDLDFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDEN 410

BLAST of Lag0034859 vs. ExPASy TrEMBL
Match: A0A6J1EIP6 (lysine-specific demethylase JMJ30-like OS=Cucurbita moschata OX=3662 GN=LOC111434618 PE=4 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 9.4e-226
Identity = 380/410 (92.68%), Postives = 392/410 (95.61%), Query Frame = 0

Query: 1   MSSSEPNQNPSSYHFETPLLDAESPSLLHSISEHGGYAYVSMAALAASGDSCAAEAAGEM 60
           MSSS     PSSYHFETPLLDAE PSLLHSI EHGGYAY+SMAALAA+GDS AAEAA EM
Sbjct: 1   MSSSGAKDGPSSYHFETPLLDAELPSLLHSICEHGGYAYISMAALAAAGDSHAAEAASEM 60

Query: 61  AWEQLHSGPWHSVLPIWRDAYSMACLYMARFHFAGGEFKEALRVLDMGLIMGGPLLRSDL 120
           AWEQLHSGPWHSVLPIWRDAYSMACLY+ARFHFAGG+FKEALRVLDMG+IMGGPLLRSDL
Sbjct: 61  AWEQLHSGPWHSVLPIWRDAYSMACLYLARFHFAGGDFKEALRVLDMGVIMGGPLLRSDL 120

Query: 121 DSAIAKVSAEARIVRVLDVNQKDAQLEPPLGCEERNKEVPWNLPVKALSNKMVAKMSALS 180
           DSAIAKVSA AR+VRVLDV +KD QLEPPLGCEERN EVP NLPVKAL+NK+VAKMSALS
Sbjct: 121 DSAIAKVSAAARVVRVLDVAEKDDQLEPPLGCEERNNEVPSNLPVKALTNKIVAKMSALS 180

Query: 181 LEGFLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQ 240
           LEGFLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQ
Sbjct: 181 LEGFLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQ 240

Query: 241 ELITFSEFLGRIQLNDCSDDITYLAQHPLFDQIDELQKDICIPDYCFVGGGELRSLNAWF 300
           ELITFSEFL RIQ N+ SDDITYLAQHPLFDQIDEL+KDICIPDYCFVGGGELRSLNAWF
Sbjct: 241 ELITFSEFLVRIQSNNRSDDITYLAQHPLFDQIDELRKDICIPDYCFVGGGELRSLNAWF 300

Query: 301 GPPGTVTPLHHDPHHNILAQVVGKKYIRLYDASLSEELYPYTETMLCNSSQVDLDNIDEK 360
           GPPGTVTPLHHDPHHNILAQVVGKKYIRLYDASL EELYPYTETMLCNSSQVDLDNIDEK
Sbjct: 301 GPPGTVTPLHHDPHHNILAQVVGKKYIRLYDASLCEELYPYTETMLCNSSQVDLDNIDEK 360

Query: 361 EFPKVVDLEFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDEN 411
           EFPKVVDL+FVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDEN
Sbjct: 361 EFPKVVDLDFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDEN 410

BLAST of Lag0034859 vs. ExPASy TrEMBL
Match: A0A5A7UEA3 (Putative lysine-specific demethylase JMJD5 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold204G00210 PE=4 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 1.4e-221
Identity = 375/414 (90.58%), Postives = 392/414 (94.69%), Query Frame = 0

Query: 1   MSSSEPNQNPSSYHFETPLLDAESPSLLHSISEHGGYAYVSMAALAASGDSCAAEAAGEM 60
           MSSSEP ++PSS+HFETPLLDAES SLLHSISEHGGYAYVSM ALAA+GDS AAEAAGEM
Sbjct: 1   MSSSEPKRSPSSFHFETPLLDAESHSLLHSISEHGGYAYVSMVALAAAGDSRAAEAAGEM 60

Query: 61  AWEQLHSGPWHSVLPIWRDAYSMACLYMARFHFAGGEFKEALRVLDMGLIMGGPLLRSDL 120
           AWEQLHSGPWHSVLPIWRDAYSMACLY+ARFHFAGGEFKEALRVLDMG+IMGGPL R+DL
Sbjct: 61  AWEQLHSGPWHSVLPIWRDAYSMACLYLARFHFAGGEFKEALRVLDMGVIMGGPLFRNDL 120

Query: 121 DSAIAKVSAEARIVRVLDVNQKDAQLEPPLGCEERNKEVPWNLPVKALSNKMVAKMSALS 180
           DSAIAKVSAEAR VRVL+  + D   E  LGCEERN EV WNLPVKAL+NKM+AKMS+LS
Sbjct: 121 DSAIAKVSAEARSVRVLEDGRVD---ESSLGCEERNNEVAWNLPVKALTNKMIAKMSSLS 180

Query: 181 LEGFLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQ 240
           LEGFLREYFQPGFPVIISDGMAHWPARTKWK+MDYLQK+AG RTIPVEVGKNYLRPEWKQ
Sbjct: 181 LEGFLREYFQPGFPVIISDGMAHWPARTKWKNMDYLQKIAGGRTIPVEVGKNYLRPEWKQ 240

Query: 241 ELITFSEFLGRIQLNDCSDDITYLAQHPLFDQIDELQKDICIPDYCFVGGGELRSLNAWF 300
           ELITFSEFL RIQ ND SDDITYLAQHPLFDQI+EL+KDICIPDYC VGGGELRSLNAWF
Sbjct: 241 ELITFSEFLSRIQSNDRSDDITYLAQHPLFDQINELRKDICIPDYCSVGGGELRSLNAWF 300

Query: 301 GPPGTVTPLHHDPHHNILAQVVGKKYIRLYDASLSEELYPYTETMLCNSSQVDLDNIDEK 360
           GPPGTVTPLHHDPHHNILAQV+GKKYIRLYDASLSEELYPYTETMLCNSSQVDLDNIDEK
Sbjct: 301 GPPGTVTPLHHDPHHNILAQVLGKKYIRLYDASLSEELYPYTETMLCNSSQVDLDNIDEK 360

Query: 361 EFPKVVDLEFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDENSTSS 415
            FPKVVDLEFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDENSTSS
Sbjct: 361 AFPKVVDLEFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDENSTSS 411

BLAST of Lag0034859 vs. ExPASy TrEMBL
Match: A0A6J1DMK3 (lysine-specific demethylase JMJ30 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111021407 PE=4 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 6.6e-219
Identity = 370/413 (89.59%), Postives = 387/413 (93.70%), Query Frame = 0

Query: 1   MSSSEPNQNPSSYHFETPLLDAESPSLLHSISEHGGYAYVSMAALAASGDSCAAEAAGEM 60
           MSSSEPN  PSS+HFETPLLDA+SPSLLHSISEHGGYA+V+MA+LAA+GDSCAAEAA EM
Sbjct: 1   MSSSEPNGAPSSFHFETPLLDAQSPSLLHSISEHGGYAFVAMASLAAAGDSCAAEAAAEM 60

Query: 61  AWEQLHSGPWHSVLPIWRDAYSMACLYMARFHFAGGEFKEALRVLDMGLIMGGPLLRSDL 120
           AWEQLHSGPWHSVLPIWRDAYSMACLYMA FHFA  EFKEALRVLDMGLIMGG LLR DL
Sbjct: 61  AWEQLHSGPWHSVLPIWRDAYSMACLYMASFHFAAREFKEALRVLDMGLIMGGALLRKDL 120

Query: 121 DSAIAKVSAEARIVRVLDVNQKDAQLEPPLGCEERNKEVPWNLPVKALSNKMVAKMSALS 180
           DSAI KVSAEARIVRVLDV Q D Q E PL  +ER+KEV WNLP KAL+NKMV KMSALS
Sbjct: 121 DSAIEKVSAEARIVRVLDVAQSDDQSERPLLYDERSKEVSWNLPGKALTNKMVTKMSALS 180

Query: 181 LEGFLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQ 240
           LEGFLRE+FQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQ
Sbjct: 181 LEGFLREHFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQ 240

Query: 241 ELITFSEFLGRIQLNDCSDDITYLAQHPLFDQIDELQKDICIPDYCFVGGGELRSLNAWF 300
           ELITFSEFLGRIQ  DCSDDITYLAQHPLFDQI+EL+ DICIPDYCFVGGGELRSLNAWF
Sbjct: 241 ELITFSEFLGRIQSTDCSDDITYLAQHPLFDQINELRNDICIPDYCFVGGGELRSLNAWF 300

Query: 301 GPPGTVTPLHHDPHHNILAQVVGKKYIRLYDASLSEELYPYTETMLCNSSQVDLDNIDEK 360
           GP GTVTPLHHDPHHNILAQV+GKKYIRLYDASLSEELYPYTETMLCNSSQVDLD+ID K
Sbjct: 301 GPAGTVTPLHHDPHHNILAQVLGKKYIRLYDASLSEELYPYTETMLCNSSQVDLDSIDGK 360

Query: 361 EFPKVVDLEFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDENSTS 414
           EFPK VDLEFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNC+ ++TS
Sbjct: 361 EFPKAVDLEFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCENSTTS 413

BLAST of Lag0034859 vs. ExPASy TrEMBL
Match: A0A6J1JIR3 (lysine-specific demethylase JMJ30-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485469 PE=4 SV=1)

HSP 1 Score: 728.8 bits (1880), Expect = 1.3e-206
Identity = 350/414 (84.54%), Postives = 378/414 (91.30%), Query Frame = 0

Query: 1   MSSSEPNQNPSSYHFETPLLDAESPSLLHSISEHGGYAYVSMAALAASGDSCAAEAAGEM 60
           MSSSE  Q PSSYHFETPLLDA+ PSLLHSIS+ GGYA+V+MAALAA+GDSCAAE A +M
Sbjct: 1   MSSSESKQTPSSYHFETPLLDAQHPSLLHSISDQGGYAFVTMAALAAAGDSCAAETAADM 60

Query: 61  AWEQLHSGPWHSVLPIWRDAYSMACLYMARFHFAGGEFKEALRVLDMGLIMGGPLLRSDL 120
           AWEQLHSGP  SVLPIWRDAYSMACLYMAR HF+GGEFKEALRVLDMGLIMGG  LRSDL
Sbjct: 61  AWEQLHSGPSSSVLPIWRDAYSMACLYMARLHFSGGEFKEALRVLDMGLIMGGSRLRSDL 120

Query: 121 DSAIAKVSAEARIVRVLDVNQKDAQLEPPLGCEERNKEVPWNLPVKALSNKMVAKMSALS 180
           DSAIAKVSAEARIVRVL     D  +E  LGC+E N+EV WNLPVK+L+NKMV KMS LS
Sbjct: 121 DSAIAKVSAEARIVRVL-----DGHVESSLGCDECNEEVAWNLPVKSLTNKMVVKMSGLS 180

Query: 181 LEGFLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQ 240
           LEGFLREYFQPGFPVI+SDGMAHWPARTKWKS+DYL +VAGDRTIPVEVGK+YLRP+WKQ
Sbjct: 181 LEGFLREYFQPGFPVIVSDGMAHWPARTKWKSLDYLHRVAGDRTIPVEVGKSYLRPQWKQ 240

Query: 241 ELITFSEFLGRIQLNDCSDDITYLAQHPLFDQIDELQKDICIPDYCFVGGGELRSLNAWF 300
           +LITFSEFL RIQ ND S DITYLA HPLFDQI+EL++DIC+PDYC VGGGELRSLNAWF
Sbjct: 241 QLITFSEFLDRIQSNDPSHDITYLAHHPLFDQINELREDICVPDYCSVGGGELRSLNAWF 300

Query: 301 GPPGTVTPLHHDPHHNILAQVVGKKYIRLYDASLSEELYPYTETMLCNSSQVDLDNIDEK 360
           GP G V+PLHHDPHHN+LAQV+GKKYIRLYDASLSEELYPYTETMLCNSSQVDLDNID+K
Sbjct: 301 GPAGIVSPLHHDPHHNMLAQVLGKKYIRLYDASLSEELYPYTETMLCNSSQVDLDNIDKK 360

Query: 361 EFPKVVDLEFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDENSTSS 415
           EF KVVDLEFVDCILEEGEMLYIPPKWWHY RSLTTSFSVSFWWNNCDENS++S
Sbjct: 361 EFGKVVDLEFVDCILEEGEMLYIPPKWWHYGRSLTTSFSVSFWWNNCDENSSAS 409

BLAST of Lag0034859 vs. TAIR 10
Match: AT3G20810.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 567.8 bits (1462), Expect = 7.3e-162
Identity = 279/402 (69.40%), Postives = 325/402 (80.85%), Query Frame = 0

Query: 17  TPLLDAESPSLLHSISEHGGYAYVSMAALAASGDSCAAEAAGEMAWEQLHSGPWHSVLPI 76
           TP LDAES +LL SIS  GGYAY  MA LA +GD  AAEAA +MAWEQLHSGPWHSVLP+
Sbjct: 19  TPTLDAESQTLLQSISAEGGYAYARMAVLAVAGDQSAAEAARDMAWEQLHSGPWHSVLPV 78

Query: 77  WRDAYSMACLYMARFHFAGGEFKEALRVLDMGLIMGGPLLRSDLDSAIAKVSAEAR-IVR 136
           WRDAYSMACL++A+ HFA GEF EAL  LDMGLIMGG LLR DL  ++  VS+EAR + +
Sbjct: 79  WRDAYSMACLHVAKIHFAAGEFGEALGALDMGLIMGGMLLRKDLHDSVLLVSSEARKMTK 138

Query: 137 VLDV---NQKDAQLEPPLGCEERNKEVPWNLPVKALSNKMVAKMSALSLEGFLREYFQPG 196
            L+    + K  +L P +  +    EV   LP ++L+ K V K S LSLEGFLR+Y+ PG
Sbjct: 139 SLEEASGDFKGERLVPEVPVDV--NEVLKILPCRSLTCKRVEKRSGLSLEGFLRDYYLPG 198

Query: 197 FPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQELITFSEFLGRI 256
            PV+I++ MAHWPARTKW  +DYL  VAG+RT+PVEVGKNYL  +WKQEL+TFS+FL R+
Sbjct: 199 TPVVITNSMAHWPARTKWNHLDYLNAVAGNRTVPVEVGKNYLCSDWKQELVTFSKFLERM 258

Query: 257 QLNDCSD-DITYLAQHPLFDQIDELQKDICIPDYCFVGGGELRSLNAWFGPPGTVTPLHH 316
           + N  S  + TYLAQHPLFDQI+EL+ DICIPDYCFVGGGEL+SLNAWFGP GTVTPLHH
Sbjct: 259 RTNKSSPMEPTYLAQHPLFDQINELRDDICIPDYCFVGGGELQSLNAWFGPAGTVTPLHH 318

Query: 317 DPHHNILAQVVGKKYIRLYDASLSEELYPYTETMLCNSSQVDLDNIDEKEFPKVVDLEFV 376
           DPHHNILAQVVGKKYIRLY + L +ELYPY+ETMLCNSSQVDLDNIDE EFPK ++LEF+
Sbjct: 319 DPHHNILAQVVGKKYIRLYPSFLQDELYPYSETMLCNSSQVDLDNIDETEFPKAMELEFM 378

Query: 377 DCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDENSTS 414
           DCILEEGEMLYIPPKWWHYVRSLT S SVSFWW+N  E+S+S
Sbjct: 379 DCILEEGEMLYIPPKWWHYVRSLTMSLSVSFWWSNEAESSSS 418

BLAST of Lag0034859 vs. TAIR 10
Match: AT3G20810.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 563.1 bits (1450), Expect = 1.8e-160
Identity = 279/411 (67.88%), Postives = 326/411 (79.32%), Query Frame = 0

Query: 17  TPLLDAESPSLLHSISEHGGYAYVSMAALAASGDSCAAEAAGEMAWEQLHSGPWHSVLPI 76
           TP LDAES +LL SIS  GGYAY  MA LA +GD  AAEAA +MAWEQLHSGPWHSVLP+
Sbjct: 19  TPTLDAESQTLLQSISAEGGYAYARMAVLAVAGDQSAAEAARDMAWEQLHSGPWHSVLPV 78

Query: 77  WRDAYSMACLYMARFHFAGGEFKEALRVLDMGLIMGGPLLRSDLDSAIAKVSAEAR-IVR 136
           WRDAYSMACL++A+ HFA GEF EAL  LDMGLIMGG LLR DL  ++  VS+EAR + +
Sbjct: 79  WRDAYSMACLHVAKIHFAAGEFGEALGALDMGLIMGGMLLRKDLHDSVLLVSSEARKMTK 138

Query: 137 VLDV---NQKDAQLEPPLGCEERN-KEVPWN--------LPVKALSNKMVAKMSALSLEG 196
            L+    + K  +L P +  +    + V  N        LP ++L+ K V K S LSLEG
Sbjct: 139 SLEEASGDFKGERLVPEVPVDVNEVRHVLANLQLLVLKILPCRSLTCKRVEKRSGLSLEG 198

Query: 197 FLREYFQPGFPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQELI 256
           FLR+Y+ PG PV+I++ MAHWPARTKW  +DYL  VAG+RT+PVEVGKNYL  +WKQEL+
Sbjct: 199 FLRDYYLPGTPVVITNSMAHWPARTKWNHLDYLNAVAGNRTVPVEVGKNYLCSDWKQELV 258

Query: 257 TFSEFLGRIQLNDCSD-DITYLAQHPLFDQIDELQKDICIPDYCFVGGGELRSLNAWFGP 316
           TFS+FL R++ N  S  + TYLAQHPLFDQI+EL+ DICIPDYCFVGGGEL+SLNAWFGP
Sbjct: 259 TFSKFLERMRTNKSSPMEPTYLAQHPLFDQINELRDDICIPDYCFVGGGELQSLNAWFGP 318

Query: 317 PGTVTPLHHDPHHNILAQVVGKKYIRLYDASLSEELYPYTETMLCNSSQVDLDNIDEKEF 376
            GTVTPLHHDPHHNILAQVVGKKYIRLY + L +ELYPY+ETMLCNSSQVDLDNIDE EF
Sbjct: 319 AGTVTPLHHDPHHNILAQVVGKKYIRLYPSFLQDELYPYSETMLCNSSQVDLDNIDETEF 378

Query: 377 PKVVDLEFVDCILEEGEMLYIPPKWWHYVRSLTTSFSVSFWWNNCDENSTS 414
           PK ++LEF+DCILEEGEMLYIPPKWWHYVRSLT S SVSFWW+N  E+S+S
Sbjct: 379 PKAMELEFMDCILEEGEMLYIPPKWWHYVRSLTMSLSVSFWWSNEAESSSS 429

BLAST of Lag0034859 vs. TAIR 10
Match: AT3G20810.3 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 542.3 bits (1396), Expect = 3.3e-154
Identity = 265/381 (69.55%), Postives = 308/381 (80.84%), Query Frame = 0

Query: 17  TPLLDAESPSLLHSISEHGGYAYVSMAALAASGDSCAAEAAGEMAWEQLHSGPWHSVLPI 76
           TP LDAES +LL SIS  GGYAY  MA LA +GD  AAEAA +MAWEQLHSGPWHSVLP+
Sbjct: 19  TPTLDAESQTLLQSISAEGGYAYARMAVLAVAGDQSAAEAARDMAWEQLHSGPWHSVLPV 78

Query: 77  WRDAYSMACLYMARFHFAGGEFKEALRVLDMGLIMGGPLLRSDLDSAIAKVSAEAR-IVR 136
           WRDAYSMACL++A+ HFA GEF EAL  LDMGLIMGG LLR DL  ++  VS+EAR + +
Sbjct: 79  WRDAYSMACLHVAKIHFAAGEFGEALGALDMGLIMGGMLLRKDLHDSVLLVSSEARKMTK 138

Query: 137 VLDV---NQKDAQLEPPLGCEERNKEVPWNLPVKALSNKMVAKMSALSLEGFLREYFQPG 196
            L+    + K  +L P +  +    EV   LP ++L+ K V K S LSLEGFLR+Y+ PG
Sbjct: 139 SLEEASGDFKGERLVPEVPVDV--NEVLKILPCRSLTCKRVEKRSGLSLEGFLRDYYLPG 198

Query: 197 FPVIISDGMAHWPARTKWKSMDYLQKVAGDRTIPVEVGKNYLRPEWKQELITFSEFLGRI 256
            PV+I++ MAHWPARTKW  +DYL  VAG+RT+PVEVGKNYL  +WKQEL+TFS+FL R+
Sbjct: 199 TPVVITNSMAHWPARTKWNHLDYLNAVAGNRTVPVEVGKNYLCSDWKQELVTFSKFLERM 258

Query: 257 QLNDCSD-DITYLAQHPLFDQIDELQKDICIPDYCFVGGGELRSLNAWFGPPGTVTPLHH 316
           + N  S  + TYLAQHPLFDQI+EL+ DICIPDYCFVGGGEL+SLNAWFGP GTVTPLHH
Sbjct: 259 RTNKSSPMEPTYLAQHPLFDQINELRDDICIPDYCFVGGGELQSLNAWFGPAGTVTPLHH 318

Query: 317 DPHHNILAQVVGKKYIRLYDASLSEELYPYTETMLCNSSQVDLDNIDEKEFPKVVDLEFV 376
           DPHHNILAQVVGKKYIRLY + L +ELYPY+ETMLCNSSQVDLDNIDE EFPK ++LEF+
Sbjct: 319 DPHHNILAQVVGKKYIRLYPSFLQDELYPYSETMLCNSSQVDLDNIDETEFPKAMELEFM 378

Query: 377 DCILEEGEMLYIPPKWWHYVR 393
           DCILEEGEMLYIPPKWWHYVR
Sbjct: 379 DCILEEGEMLYIPPKWWHYVR 397

BLAST of Lag0034859 vs. TAIR 10
Match: AT5G19840.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 94.0 bits (232), Expect = 3.1e-19
Identity = 74/275 (26.91%), Postives = 119/275 (43.27%), Query Frame = 0

Query: 177 SALSLEGFLREYFQPGFPVIISDGMAHWPARTKW----KSMDYLQKVAGDRTIPVEVGKN 236
           +AL+   F        FP +     + W A +KW      +DYL++ AG  ++ VE   +
Sbjct: 15  NALTAADFESTVELTNFPAVFRGCASVWDAYSKWNPFNSGLDYLEERAG--SVEVEAMLS 74

Query: 237 YLRPEWKQEL-------ITFSEFL------------------GRIQLNDCSDDI----TY 296
              P +  ++       + FS+F+                      LN   +D      Y
Sbjct: 75  RTAPVFNGDIRSHERVSLPFSDFIRFCKQHMRGKGNGSGVDAKSADLNPMCEDYRPGQIY 134

Query: 297 LAQHPLFDQIDE-------LQKDICIPDYCFVGGGELRSLNAWFGPPGTVTPLHHDPHHN 356
           LAQ P+ +   E       L++DI  P   F+    L S+N W       +  H+DPHHN
Sbjct: 135 LAQFPILNDEKEEKVLLKILRQDIQTP--TFLDAKSLSSINFWMNSAEARSSTHYDPHHN 194

Query: 357 ILAQVVGKKYIRLYDASLSEELYP---YTETMLCNSSQVDLDNIDEKEFPKVVD--LEFV 407
           +L  V G+K + L+  S S  LYP   Y E    N S V L+N +  ++P+      +  
Sbjct: 195 LLCVVSGRKKVVLWPPSASPSLYPMPIYGEA--SNHSSVGLENPNLSDYPRAEHSLKQSQ 254

BLAST of Lag0034859 vs. TAIR 10
Match: AT5G19840.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 94.0 bits (232), Expect = 3.1e-19
Identity = 74/275 (26.91%), Postives = 119/275 (43.27%), Query Frame = 0

Query: 177 SALSLEGFLREYFQPGFPVIISDGMAHWPARTKW----KSMDYLQKVAGDRTIPVEVGKN 236
           +AL+   F        FP +     + W A +KW      +DYL++ AG  ++ VE   +
Sbjct: 15  NALTAADFESTVELTNFPAVFRGCASVWDAYSKWNPFNSGLDYLEERAG--SVEVEAMLS 74

Query: 237 YLRPEWKQEL-------ITFSEFL------------------GRIQLNDCSDDI----TY 296
              P +  ++       + FS+F+                      LN   +D      Y
Sbjct: 75  RTAPVFNGDIRSHERVSLPFSDFIRFCKQHMRGKGNGSGVDAKSADLNPMCEDYRPGQIY 134

Query: 297 LAQHPLFDQIDE-------LQKDICIPDYCFVGGGELRSLNAWFGPPGTVTPLHHDPHHN 356
           LAQ P+ +   E       L++DI  P   F+    L S+N W       +  H+DPHHN
Sbjct: 135 LAQFPILNDEKEEKVLLKILRQDIQTP--TFLDAKSLSSINFWMNSAEARSSTHYDPHHN 194

Query: 357 ILAQVVGKKYIRLYDASLSEELYP---YTETMLCNSSQVDLDNIDEKEFPKVVD--LEFV 407
           +L  V G+K + L+  S S  LYP   Y E    N S V L+N +  ++P+      +  
Sbjct: 195 LLCVVSGRKKVVLWPPSASPSLYPMPIYGEA--SNHSSVGLENPNLSDYPRAEHSLKQSQ 254

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022967535.11.4e-22692.44lysine-specific demethylase JMJ30-like [Cucurbita maxima][more]
XP_022927851.12.0e-22592.68lysine-specific demethylase JMJ30-like [Cucurbita moschata][more]
KAG6588179.13.7e-22492.20Lysine-specific demethylase JMJ30, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023529860.13.7e-22491.95lysine-specific demethylase JMJ30-like [Cucurbita pepo subsp. pepo][more]
XP_038878408.12.4e-22391.30lysine-specific demethylase JMJ30 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q8RWR12.5e-15967.88Lysine-specific demethylase JMJ30 OS=Arabidopsis thaliana OX=3702 GN=JMJ30 PE=1 ... [more]
Q9CXT68.9e-8042.86Bifunctional peptidase and arginyl-hydroxylase JMJD5 OS=Mus musculus OX=10090 GN... [more]
Q1JP619.8e-7944.10Bifunctional peptidase and arginyl-hydroxylase JMJD5 OS=Bos taurus OX=9913 GN=KD... [more]
Q497B82.9e-7843.18Bifunctional peptidase and arginyl-hydroxylase JMJD5 OS=Rattus norvegicus OX=101... [more]
Q8N3712.4e-7743.29Bifunctional peptidase and arginyl-hydroxylase JMJD5 OS=Homo sapiens OX=9606 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1HUR96.5e-22792.44lysine-specific demethylase JMJ30-like OS=Cucurbita maxima OX=3661 GN=LOC1114670... [more]
A0A6J1EIP69.4e-22692.68lysine-specific demethylase JMJ30-like OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A5A7UEA31.4e-22190.58Putative lysine-specific demethylase JMJD5 isoform X1 OS=Cucumis melo var. makuw... [more]
A0A6J1DMK36.6e-21989.59lysine-specific demethylase JMJ30 isoform X3 OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1JIR31.3e-20684.54lysine-specific demethylase JMJ30-like isoform X1 OS=Cucurbita maxima OX=3661 GN... [more]
Match NameE-valueIdentityDescription
AT3G20810.17.3e-16269.402-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G20810.21.8e-16067.882-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G20810.33.3e-15469.552-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G19840.13.1e-1926.912-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G19840.23.1e-1926.912-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003347JmjC domainSMARTSM00558cupin_9coord: 262..413
e-value: 2.9E-10
score: 50.1
IPR003347JmjC domainPROSITEPS51184JMJCcoord: 256..414
score: 27.607035
IPR041667Cupin-like domain 8PFAMPF13621Cupin_8coord: 184..407
e-value: 2.7E-48
score: 165.0
NoneNo IPR availableGENE3D2.60.120.650Cupincoord: 172..405
e-value: 2.9E-83
score: 281.1
NoneNo IPR availablePANTHERPTHR12461:SF86SUBFAMILY NOT NAMEDcoord: 40..410
NoneNo IPR availablePANTHERPTHR12461HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATEDcoord: 40..410
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 176..406

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0034859.1Lag0034859.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0008168 methyltransferase activity