Lag0034854 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0034854
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	cytochrome P450 CYP736A12-like
Location	chr3: 11656830 .. 11658707 (-)
RNA-Seq Expression	Lag0034854
Synteny	Lag0034854

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTGGATTTGGATCATCATCATTCATCTGCTTGCTCTTGCACTTGTCTTTGGGCCATGGCTGTTGAGAAACAAAAACAAGAAACTTCCTCCTGGTCCAAAAGGGTTTCCAATTTTCGGTAGCCTTCATCTGTTGGGAAAGCTTCCTCACAGAGATCTCCATCGCTTGTCTCAGAAATATGGAGCCATCATGCACATGAAGCTCGGCCTTGTAAACACCATCGTTGTCTCTTCCCCTGAAGCTGCAGAGCTCTTTCTCAAAACCCACGACCTTGTTTTTGCAAGCCGCCCACCTAGTGAGGCTTTCAAGCACATGTCATACGGTCAAAAGAGCATGGTGTTTGCAAAATATGGCCCTTATTGGCGCAACATGCGCAAGATGTTCACGCTCGAGTTGCTCAGCAGCCACAAAGCCAATTCCTTCAAATCAATGAGAAGGGAAGAGCTTAGCTCGTTGATTGACGATCTTCGTGAGGCTGCAAAGAGTGGCGCGGTTGTGGATCTTACTTCCAAAGTCTGTGCTCTCAGTGCAAACATCACGTGTCTGATGATCTTTGGGAGAAAATTTGAAGATGAAGAACTCGATGAGAGAGGCTTCAAGTGGATGACACAAGAGGCCATGAGATTGGGAGGAGCTTTCAACTTGGGGGACTTCGTTCCTTTTATTGCAGTTCTTGATGTTGGAGGATTGAACCGCCGTGCAAAATCTGTTGCTAAAGTATTTGATAGGTTTTTTGAAAGGATTATTGATGAACATCTTCAATCATCTAAGAATGATGCTACCAAAGATTTCGTACATGTCATGTTGGACATTATGGGTTCTAAAGAAACTGAGTATCAAATTGATCGATCTAACATCAAGGCCCTAATGCTAGTGAGTAAACTTTTCTTCCATTCATACCTTCTCTTTGTTGAGGATCCCACGTTGAAAAGATTAGTGGAGACCTCACAATATATAAGCTTCAAGGGTCACTCCACTCATCACTAATTGGTTTTGAGATGGAAGTCCATATAATCTAATATGGTATCAGAGCTCATTAAACCCAAAAAAGATTTCTAATTAACCAGAGTCTATCAGTAATAATAGATTTTGCTATATTTGTAATTATATAATTCTTTATATAATTCTTTTTTGCCACCCACTACAAATTCTCATATATATATATATGTGTGTGTGTGTGGCTTTTACAATATTAATACGATTATCTTTTTATAATTCTATAATAAATTATGAATATTTTCTCCTTTAGGATTTGCTTCTTGGGGCAATGGACACTTCAGCCACCACCATCGGATGGATAGTAGCTGAGCTCATTAGACATCCACGAGTAATGAAGAAAGTGCAAGAAGAATTAGAAAAAGTTGTGGGTTTGAATAGAATGGTGCAAGAAACAGACTTAGATCACTTGGAATACTTGAAAATGGTAGTTAAAGAGGTTTTCAGACTGCATCCTCCTGGTCCACTACTCGCTCCGCATGAGTCGCTTGAAGATTGTATAGTCAATCATTTCTATATTCCTAAAAATCACGTATAGTAGTGAATGCATGGGCAATTGGACGAAATCCAAGTGCTTGGACTGATGCAGAAAATTTCTTTCCTGAGAGATTTATCGGAAATCAGGTGGACCTAAAAGGAAGAGATTTTCAACTAATTCCATTTGGATCGGGTCGAAGAGGTTGTCCAGGAATACAAATGGGTCTTGTCGTAGTTCATTTAGTAATAGCTCAACTTGTGCATTGTTTTGATTGGAAGCTTCCAAATGGTATGTCGCCAGCTGAATCGAATATGACCGAAGAGTTTGGTTTAACTTGTTCTATCGCTCAAGATATTATGGTTACTCCTATTTACCGTCTTCGAGACGTGATAAGTTCTTGA

mRNA sequence

ATGGCTTGGATTTGGATCATCATCATTCATCTGCTTGCTCTTGCACTTGTCTTTGGGCCATGGCTGTTGAGAAACAAAAACAAGAAACTTCCTCCTGGTCCAAAAGGGTTTCCAATTTTCGGTAGCCTTCATCTGTTGGGAAAGCTTCCTCACAGAGATCTCCATCGCTTGTCTCAGAAATATGGAGCCATCATGCACATGAAGCTCGGCCTTGTAAACACCATCGTTGTCTCTTCCCCTGAAGCTGCAGAGCTCTTTCTCAAAACCCACGACCTTGTTTTTGCAAGCCGCCCACCTAGTGAGGCTTTCAAGCACATGTCATACGGTCAAAAGAGCATGGTGTTTGCAAAATATGGCCCTTATTGGCGCAACATGCGCAAGATGTTCACGCTCGAGTTGCTCAGCAGCCACAAAGCCAATTCCTTCAAATCAATGAGAAGGGAAGAGCTTAGCTCGTTGATTGACGATCTTCGTGAGGCTGCAAAGAGTGGCGCGGTTGTGGATCTTACTTCCAAAGTCTGTGCTCTCAGTGCAAACATCACGTGTCTGATGATCTTTGGGAGAAAATTTGAAGATGAAGAACTCGATGAGAGAGGCTTCAAGTGGATGACACAAGAGGCCATGAGATTGGGAGGAGCTTTCAACTTGGGGGACTTCGTTCCTTTTATTGCAGTTCTTGATGTTGGAGGATTGAACCGCCGTGCAAAATCTGTTGCTAAAGTATTTGATAGGTTTTTTGAAAGGATTATTGATGAACATCTTCAATCATCTAAGAATGATGCTACCAAAGATTTCGTACATGTCATGTTGGACATTATGGGTTCTAAAGAAACTGAGTATCAAATTGATCGATCTAACATCAAGGCCCTAATGCTAGATTTGCTTCTTGGGGCAATGGACACTTCAGCCACCACCATCGGATGGATAGTAGCTGAGCTCATTAGACATCCACGAGTAATGAAGAAAGTGCAAGAAGAATTAGAAAAAGTTGTGGGTTTGAATAGAATGGTGCAAGAAACAGACTTAGATCACTTGGAATACTTGAAAATGGTAGTTAAAGAGGTTTTCAGACTGCATCCTCCTGGTCCACTACTCGCTCCGCATGAGTCGCTTGAAGATTGTATAGTCAATCATTTCTATATTCCTAAAAATCACGTGGACCTAAAAGGAAGAGATTTTCAACTAATTCCATTTGGATCGGGTCGAAGAGGTTGTCCAGGAATACAAATGGGTCTTGTCGTAGTTCATTTAGTAATAGCTCAACTTGTGCATTGTTTTGATTGGAAGCTTCCAAATGGTATGTCGCCAGCTGAATCGAATATGACCGAAGAGTTTGGTTTAACTTGTTCTATCGCTCAAGATATTATGGTTACTCCTATTTACCGTCTTCGAGACGTGATAAGTTCTTGA

Coding sequence (CDS)

ATGGCTTGGATTTGGATCATCATCATTCATCTGCTTGCTCTTGCACTTGTCTTTGGGCCATGGCTGTTGAGAAACAAAAACAAGAAACTTCCTCCTGGTCCAAAAGGGTTTCCAATTTTCGGTAGCCTTCATCTGTTGGGAAAGCTTCCTCACAGAGATCTCCATCGCTTGTCTCAGAAATATGGAGCCATCATGCACATGAAGCTCGGCCTTGTAAACACCATCGTTGTCTCTTCCCCTGAAGCTGCAGAGCTCTTTCTCAAAACCCACGACCTTGTTTTTGCAAGCCGCCCACCTAGTGAGGCTTTCAAGCACATGTCATACGGTCAAAAGAGCATGGTGTTTGCAAAATATGGCCCTTATTGGCGCAACATGCGCAAGATGTTCACGCTCGAGTTGCTCAGCAGCCACAAAGCCAATTCCTTCAAATCAATGAGAAGGGAAGAGCTTAGCTCGTTGATTGACGATCTTCGTGAGGCTGCAAAGAGTGGCGCGGTTGTGGATCTTACTTCCAAAGTCTGTGCTCTCAGTGCAAACATCACGTGTCTGATGATCTTTGGGAGAAAATTTGAAGATGAAGAACTCGATGAGAGAGGCTTCAAGTGGATGACACAAGAGGCCATGAGATTGGGAGGAGCTTTCAACTTGGGGGACTTCGTTCCTTTTATTGCAGTTCTTGATGTTGGAGGATTGAACCGCCGTGCAAAATCTGTTGCTAAAGTATTTGATAGGTTTTTTGAAAGGATTATTGATGAACATCTTCAATCATCTAAGAATGATGCTACCAAAGATTTCGTACATGTCATGTTGGACATTATGGGTTCTAAAGAAACTGAGTATCAAATTGATCGATCTAACATCAAGGCCCTAATGCTAGATTTGCTTCTTGGGGCAATGGACACTTCAGCCACCACCATCGGATGGATAGTAGCTGAGCTCATTAGACATCCACGAGTAATGAAGAAAGTGCAAGAAGAATTAGAAAAAGTTGTGGGTTTGAATAGAATGGTGCAAGAAACAGACTTAGATCACTTGGAATACTTGAAAATGGTAGTTAAAGAGGTTTTCAGACTGCATCCTCCTGGTCCACTACTCGCTCCGCATGAGTCGCTTGAAGATTGTATAGTCAATCATTTCTATATTCCTAAAAATCACGTGGACCTAAAAGGAAGAGATTTTCAACTAATTCCATTTGGATCGGGTCGAAGAGGTTGTCCAGGAATACAAATGGGTCTTGTCGTAGTTCATTTAGTAATAGCTCAACTTGTGCATTGTTTTGATTGGAAGCTTCCAAATGGTATGTCGCCAGCTGAATCGAATATGACCGAAGAGTTTGGTTTAACTTGTTCTATCGCTCAAGATATTATGGTTACTCCTATTTACCGTCTTCGAGACGTGATAAGTTCTTGA

Protein sequence

MAWIWIIIIHLLALALVFGPWLLRNKNKKLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQKYGAIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPPSEAFKHMSYGQKSMVFAKYGPYWRNMRKMFTLELLSSHKANSFKSMRREELSSLIDDLREAAKSGAVVDLTSKVCALSANITCLMIFGRKFEDEELDERGFKWMTQEAMRLGGAFNLGDFVPFIAVLDVGGLNRRAKSVAKVFDRFFERIIDEHLQSSKNDATKDFVHVMLDIMGSKETEYQIDRSNIKALMLDLLLGAMDTSATTIGWIVAELIRHPRVMKKVQEELEKVVGLNRMVQETDLDHLEYLKMVVKEVFRLHPPGPLLAPHESLEDCIVNHFYIPKNHVDLKGRDFQLIPFGSGRRGCPGIQMGLVVVHLVIAQLVHCFDWKLPNGMSPAESNMTEEFGLTCSIAQDIMVTPIYRLRDVISS

Homology

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BLAST of Lag0034854 vs. NCBI nr
Match: XP_022950432.1 (cytochrome P450 CYP736A12-like [Cucurbita moschata])

HSP 1 Score: 723.4 bits (1866), Expect = 1.3e-204
Identity = 357/496 (71.98%), Postives = 414/496 (83.47%), Query Frame = 0


Query: 1   MAWIWIIIIHLLALALVFGPWLLRNKNKKLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQK 60
           MAWIWIIII + AL L+F PWLL+NK K LPPGPKGFPI GSLHLLGKLPHRD+ RLSQ+
Sbjct: 1   MAWIWIIII-MFALVLLFWPWLLKNKGKNLPPGPKGFPILGSLHLLGKLPHRDIQRLSQR 60

Query: 61  YGAIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPPSEAFKHMSYGQKSMVFAKYGP 120
           YG IMHMKLGLVNTIVVSSPEAA LFLKTHDLVFASRP +E FKHMSYG++SMVF+KYG 
Sbjct: 61  YGPIMHMKLGLVNTIVVSSPEAAALFLKTHDLVFASRPTNEVFKHMSYGERSMVFSKYGH 120

Query: 121 YWRNMRKMFTLELLSSHKANSFKSMRREELSSLIDDLREAAKSGAVVDLTSKVCALSANI 180
           YWRNMRK+ TLELLS+ K N FKSMR+EELS LID+LREAAKS  VV+L+SKVC+LSAN+
Sbjct: 121 YWRNMRKICTLELLSNQKVNFFKSMRKEELSLLIDELREAAKSEVVVNLSSKVCSLSANM 180

Query: 181 TCLMIFGRKFEDEELDERGFKWMTQEAMRLGGAFNLGDFVPFIAVLDVGGLNRRAKSVAK 240
           TCLMIFG+KFED +LDERGFKW+ QEA++L  AFNLGDF+PFI+ LD+ GL +RAK V K
Sbjct: 181 TCLMIFGKKFEDRDLDERGFKWVVQEALKLAAAFNLGDFIPFISALDLQGLIQRAKCVNK 240

Query: 241 VFDRFFERIIDEHLQSSKNDATKDFVHVMLDIMGSKETEYQIDRSNIKALMLDLLLGAMD 300
           V DRFFERI+DEHL+ SKN+ +KDFV VML IMGS+ TEYQIDRS+IKA+MLD+LL A D
Sbjct: 241 VCDRFFERILDEHLE-SKNEDSKDFVDVMLGIMGSEVTEYQIDRSSIKAIMLDILLAATD 300

Query: 301 TSATTIGWIVAELIRHPRVMKKVQEELEKVVGLNRMVQETDLDHLEYLKMVVKEVFRLHP 360
           +SA TIGW VAELI+HP VMKKVQ+ELEKVVGLNRMV+ETDL HL YL+MVVKEV RLHP
Sbjct: 301 SSAVTIGWAVAELIKHPHVMKKVQDELEKVVGLNRMVEETDLSHLTYLEMVVKEVLRLHP 360

Query: 361 PGPLLAPHESLEDCIVNHFYIPK-------------------------------NHVDLK 420
           PGPL+ PHE+++DC VN+F+IPK                               + VD+K
Sbjct: 361 PGPLIVPHEAVKDCTVNNFHIPKKSRIIINVWAIGRNPSAWTDAEKFFPERFIGSQVDVK 420

Query: 421 GRDFQLIPFGSGRRGCPGIQMGLVVVHLVIAQLVHCFDWKLPNGMSPAESNMTEEFGLTC 466
           G+DFQL+PFGSGRRGCPG+Q+GLV +HL++AQLVHCFDWKLPNGM PA+ +MTE FGL+C
Sbjct: 421 GKDFQLLPFGSGRRGCPGMQVGLVTIHLILAQLVHCFDWKLPNGMLPADLDMTEAFGLSC 480

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BLAST of Lag0034854 vs. NCBI nr
Match: XP_023545010.1 (cytochrome P450 CYP736A12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 721.1 bits (1860), Expect = 6.3e-204
Identity = 357/496 (71.98%), Postives = 413/496 (83.27%), Query Frame = 0


Query: 1   MAWIWIIIIHLLALALVFGPWLLRNKNKKLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQK 60
           MAWIWIIII + AL L+F PW+LRNK K LPPGPKGFPI GSLHLLGKLPHRD+ RLSQ+
Sbjct: 1   MAWIWIIII-MFALVLLFRPWVLRNKGKNLPPGPKGFPILGSLHLLGKLPHRDIQRLSQR 60

Query: 61  YGAIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPPSEAFKHMSYGQKSMVFAKYGP 120
           YG IMHMKLGLVNTIVVSSPEAA LFLKTHDLV+ASRP +E FKHMSYG++SMVF+KYG 
Sbjct: 61  YGPIMHMKLGLVNTIVVSSPEAAALFLKTHDLVYASRPTNEVFKHMSYGERSMVFSKYGH 120

Query: 121 YWRNMRKMFTLELLSSHKANSFKSMRREELSSLIDDLREAAKSGAVVDLTSKVCALSANI 180
           YWRNMRK+ TLELLS+ K N FKSMRREELS LID+LREAAKS  VV+L+SKVC+LSAN+
Sbjct: 121 YWRNMRKICTLELLSNQKVNFFKSMRREELSLLIDELREAAKSEVVVNLSSKVCSLSANM 180

Query: 181 TCLMIFGRKFEDEELDERGFKWMTQEAMRLGGAFNLGDFVPFIAVLDVGGLNRRAKSVAK 240
           TCLMIFG+KFED ELDERGFKW+ QEA++L  AFNLGDF+PFI+ LD+ GL +RAK V K
Sbjct: 181 TCLMIFGKKFEDRELDERGFKWVVQEALKLAAAFNLGDFIPFISALDLQGLIQRAKCVNK 240

Query: 241 VFDRFFERIIDEHLQSSKNDATKDFVHVMLDIMGSKETEYQIDRSNIKALMLDLLLGAMD 300
           V DRFFERI+DEHL+ SKN+ +KDFV VML IMGS+ TEYQIDRS+IKA+MLD+LL A D
Sbjct: 241 VCDRFFERILDEHLE-SKNEDSKDFVDVMLGIMGSEVTEYQIDRSSIKAIMLDILLAATD 300

Query: 301 TSATTIGWIVAELIRHPRVMKKVQEELEKVVGLNRMVQETDLDHLEYLKMVVKEVFRLHP 360
           +SA TIGW VAELI+HP VMKKVQ+ELEKVVGLNRMV+ETDL HL YL+MVVKEV RLHP
Sbjct: 301 SSAVTIGWAVAELIKHPHVMKKVQDELEKVVGLNRMVEETDLCHLTYLEMVVKEVLRLHP 360

Query: 361 PGPLLAPHESLEDCIVNHFYIPK-------------------------------NHVDLK 420
           PGPL+ PHE+++DC VN+F+IPK                               + VD+K
Sbjct: 361 PGPLIVPHEAVKDCTVNNFHIPKKSRIIINVWAIGRNPSAWTDAEKFFPERFIGSQVDVK 420

Query: 421 GRDFQLIPFGSGRRGCPGIQMGLVVVHLVIAQLVHCFDWKLPNGMSPAESNMTEEFGLTC 466
           G+DFQL+PFGSGRRGCPG+Q+GLV VHL++A+LVHCFDWKLPNGM P + +MTE FGL+C
Sbjct: 421 GKDFQLLPFGSGRRGCPGMQVGLVTVHLILAELVHCFDWKLPNGMLPVDLDMTEAFGLSC 480

[Close]

BLAST of Lag0034854 vs. NCBI nr
Match: XP_022978479.1 (cytochrome P450 CYP736A12-like [Cucurbita maxima])

HSP 1 Score: 713.4 bits (1840), Expect = 1.3e-201
Identity = 354/496 (71.37%), Postives = 411/496 (82.86%), Query Frame = 0


Query: 1   MAWIWIIIIHLLALALVFGPWLLRNKNKKLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQK 60
           MAWIW+III + AL L+F  WLL+NK K LPPGPKGFPI GSLHLLGKLPHRD+ RLSQ+
Sbjct: 1   MAWIWMIII-MFALVLLFRSWLLKNKAKNLPPGPKGFPILGSLHLLGKLPHRDIQRLSQR 60

Query: 61  YGAIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPPSEAFKHMSYGQKSMVFAKYGP 120
           YG IMHMKLGLVNTIVVSSPEAA LFLKTHDLVFASRP ++ FKHMSYG++SMVF+KYG 
Sbjct: 61  YGPIMHMKLGLVNTIVVSSPEAAALFLKTHDLVFASRPQNQVFKHMSYGERSMVFSKYGH 120

Query: 121 YWRNMRKMFTLELLSSHKANSFKSMRREELSSLIDDLREAAKSGAVVDLTSKVCALSANI 180
           YWRNMRK+ TLELLS+ K N FKSMRREELS LID+LREAAKS  VV+L+SKVC+LSAN+
Sbjct: 121 YWRNMRKICTLELLSNQKVNFFKSMRREELSLLIDELREAAKSEVVVNLSSKVCSLSANM 180

Query: 181 TCLMIFGRKFEDEELDERGFKWMTQEAMRLGGAFNLGDFVPFIAVLDVGGLNRRAKSVAK 240
           TCLMIFG+KFED +LDERGFKW+ QEA++L  AFNLGDF+PFI+ LD+ GL +RAK V K
Sbjct: 181 TCLMIFGKKFEDRDLDERGFKWVVQEALKLAAAFNLGDFIPFISALDLQGLIQRAKCVNK 240

Query: 241 VFDRFFERIIDEHLQSSKNDATKDFVHVMLDIMGSKETEYQIDRSNIKALMLDLLLGAMD 300
           V DRFFERI+DEHL+ SKND +KDFV VML IMGS+ TEYQIDRS+IKA+MLD+LL A D
Sbjct: 241 VCDRFFERILDEHLE-SKNDDSKDFVDVMLGIMGSEVTEYQIDRSSIKAIMLDILLAATD 300

Query: 301 TSATTIGWIVAELIRHPRVMKKVQEELEKVVGLNRMVQETDLDHLEYLKMVVKEVFRLHP 360
           +SA TIGW VAELI+HP VMK VQ+ELEKVVGLNRMV+ETDL HL YL++VVKEV RLHP
Sbjct: 301 SSAVTIGWAVAELIKHPYVMKNVQDELEKVVGLNRMVEETDLCHLTYLEIVVKEVLRLHP 360

Query: 361 PGPLLAPHESLEDCIVNHFYIPK-------------------------------NHVDLK 420
           PGPL+ PHE+++DC VN+F+IPK                               + VD+K
Sbjct: 361 PGPLIVPHEAVKDCTVNNFHIPKKSRIIINVWAIGRNPSAWTDAEKFFPERFIGSQVDVK 420

Query: 421 GRDFQLIPFGSGRRGCPGIQMGLVVVHLVIAQLVHCFDWKLPNGMSPAESNMTEEFGLTC 466
           G+DFQL+PFGSGRRGCPGIQ+GLV VHL++AQL+HCFDWKLPNGM PA+ +M E FGL+C
Sbjct: 421 GKDFQLLPFGSGRRGCPGIQVGLVTVHLILAQLIHCFDWKLPNGMLPADLDMMEAFGLSC 480

[Close]

BLAST of Lag0034854 vs. NCBI nr
Match: XP_023544864.1 (cytochrome P450 CYP736A12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 684.9 bits (1766), Expect = 5.0e-193
Identity = 338/496 (68.15%), Postives = 398/496 (80.24%), Query Frame = 0


Query: 1   MAWIWIIIIHLLALALVFGPWLLRNKNKKLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQK 60
           MAWIW+III + AL L+F  W+ RNK+KKLPPGPKGFPI GSLHLLGKLPHRDLHRLSQ 
Sbjct: 1   MAWIWMIII-MFALVLLFWAWMERNKSKKLPPGPKGFPIMGSLHLLGKLPHRDLHRLSQT 60

Query: 61  YGAIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPPSEAFKHMSYGQKSMVFAKYGP 120
           YG IMHMKLG+VNTIVVSSPEAAELFLKTHD+VFASR P+E FKH+S+ QK++ FAKYGP
Sbjct: 61  YGPIMHMKLGIVNTIVVSSPEAAELFLKTHDVVFASRAPNEVFKHVSFSQKTIAFAKYGP 120

Query: 121 YWRNMRKMFTLELLSSHKANSFKSMRREELSSLIDDLREAAKSGAVVDLTSKVCALSANI 180
           YWRNMRKM  LELLS+HKAN  +SMRR+EL   I  LR+AAKSG VV+++SK+ +L+AN+
Sbjct: 121 YWRNMRKMCILELLSNHKANYLQSMRRQELGFTIHQLRDAAKSGMVVNISSKIYSLTANM 180

Query: 181 TCLMIFGRKFEDEELDERGFKWMTQEAMRLGGAFNLGDFVPFIAVLDVGGLNRRAKSVAK 240
            CLMI GR+FED+ELDE GFKWM QE + L GAFNLGDF+PFIA LD+ G  R A+SV K
Sbjct: 181 ACLMIIGRRFEDKELDEMGFKWMIQEILHLAGAFNLGDFIPFIAPLDLQGFIRCARSVHK 240

Query: 241 VFDRFFERIIDEHLQSSKNDATKDFVHVMLDIMGSKETEYQIDRSNIKALMLDLLLGAMD 300
           V D FFE+I+DEHL+S+ ND TKD V VML IMGS+ TEYQID S IKA++LD+LLG ++
Sbjct: 241 VCDTFFEKILDEHLESNNND-TKDLVDVMLGIMGSQATEYQIDPSTIKAIILDMLLGGVE 300

Query: 301 TSATTIGWIVAELIRHPRVMKKVQEELEKVVGLNRMVQETDLDHLEYLKMVVKEVFRLHP 360
           TSATTI W +AEL+RHP+ MKKVQ+EL+KVVGLNRMVQE DLDHLEYLKMVVKE FRLHP
Sbjct: 301 TSATTIEWALAELVRHPQTMKKVQDELQKVVGLNRMVQEADLDHLEYLKMVVKETFRLHP 360

Query: 361 PGPLLAPHESLEDCIVNHFYIPK-------------------------------NHVDLK 420
           P PLL P ES++D ++N+FYIP+                                 VD+K
Sbjct: 361 PNPLLIPRESVKDSVINNFYIPQKSRIILNVWAIGRDPSVWIDAEKFFPERFIGTQVDVK 420

Query: 421 GRDFQLIPFGSGRRGCPGIQMGLVVVHLVIAQLVHCFDWKLPNGMSPAESNMTEEFGLTC 466
           GRDFQL+PFGSGRRGCPGIQMGLV VHL++AQLVHCFDWKLPNGM P E +MTE+FGL+C
Sbjct: 421 GRDFQLLPFGSGRRGCPGIQMGLVTVHLILAQLVHCFDWKLPNGMLPDELDMTEKFGLSC 480

[Close]

BLAST of Lag0034854 vs. NCBI nr
Match: XP_022950433.1 (cytochrome P450 CYP736A12-like [Cucurbita moschata])

HSP 1 Score: 684.5 bits (1765), Expect = 6.5e-193
Identity = 346/497 (69.62%), Postives = 399/497 (80.28%), Query Frame = 0


Query: 1   MAWIWIIIIHLLALALVFGPWLLRNKNKKLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQK 60
           MAWIWIIII +  L L+F  WL RN+++KLPPGPKGFPI GSLHLLGKLPHRDLHRLSQ 
Sbjct: 1   MAWIWIIII-MFTLVLLFWAWLGRNQSQKLPPGPKGFPILGSLHLLGKLPHRDLHRLSQT 60

Query: 61  YGAIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPPSEAFKHMSYGQKSMVFAKYGP 120
           YG IMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASR P+E FKH+S+GQK+MVFAKYGP
Sbjct: 61  YGPIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRAPNEVFKHISFGQKTMVFAKYGP 120

Query: 121 YWRNMRKMFTLELLSSHKANSFKSMRREELSSLIDDLR-EAAKSGAVVDLTSKVCALSAN 180
           YWRNM KM  LELLS+HK +SFKSMRR+EL  LID LR +AA S  VV+LTSK+ +L+ N
Sbjct: 121 YWRNMHKMCVLELLSNHKIDSFKSMRRQELGVLIDQLRDDAANSRVVVNLTSKIYSLTIN 180

Query: 181 ITCLMIFGRKFEDEELDERGFKWMTQEAMRLGGAFNLGDFVPFIAVLDVGGLNRRAKSVA 240
           + CLM+ GRKFED+E+DERGFKWM QE   L GAFNLGDF+PFIA LD+ G   RA+SV 
Sbjct: 181 MACLMVLGRKFEDKEIDERGFKWMIQEIFHLAGAFNLGDFIPFIAPLDLQGFTPRARSVH 240

Query: 241 KVFDRFFERIIDEHLQSSKNDATKDFVHVMLDIMGSKETEYQIDRSNIKALMLDLLLGAM 300
           KV D  FE+I+DEHL+S+ N  TKDFV VML IMGS++     DRSNIKA+MLD+LLGA+
Sbjct: 241 KVCDTLFEKILDEHLESNHNH-TKDFVDVMLGIMGSQQ-----DRSNIKAIMLDMLLGAV 300

Query: 301 DTSATTIGWIVAELIRHPRVMKKVQEELEKVVGLNRMVQETDLDHLEYLKMVVKEVFRLH 360
           DT+ T+IGW +A LI HP+VMKKVQ+ELEKVVGLNRMVQE DLDHLEYL+M VKEV RLH
Sbjct: 301 DTATTSIGWALAHLITHPQVMKKVQDELEKVVGLNRMVQEEDLDHLEYLEMTVKEVLRLH 360

Query: 361 PPGPLLAPHESLEDCIVNHFYIPK-------------------------------NHVDL 420
           PP PLL PHES++DCIVN+FYIPK                               + V++
Sbjct: 361 PPIPLLIPHESVKDCIVNNFYIPKRSRMIVNVWTIGQDPSVWNDPERFFLERFMGSKVEM 420

Query: 421 KGRDFQLIPFGSGRRGCPGIQMGLVVVHLVIAQLVHCFDWKLPNGMSPAESNMTEEFGLT 466
           KGRDFQLIPFGSGRRGCPG+Q+GL+ VHLV+AQLVHCFDWKLPNG  P E +MTE+FGLT
Sbjct: 421 KGRDFQLIPFGSGRRGCPGMQLGLIAVHLVLAQLVHCFDWKLPNGKLPFELDMTEKFGLT 480

[Close]

BLAST of Lag0034854 vs. ExPASy Swiss-Prot
Match: A0A068Q5V6 (Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 5.4e-166
Identity = 290/496 (58.47%), Postives = 372/496 (75.00%), Query Frame = 0


Query: 1   MAWIWIIIIHLLALALVFGPWLLRNKNKKLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQK 60
           M WIW   I LLAL  +   W  +NK K+LPPGP+GFPIFGSLHLLG+ P++DLHRL++K
Sbjct: 1   MVWIW-ATIGLLALVHILQAW-WKNKKKRLPPGPRGFPIFGSLHLLGEFPNKDLHRLARK 60

Query: 61  YGAIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPPSEAFKHMSYGQKSMVFAKYGP 120
           YG IM+M+LGL+ TIV+SSPEAAELFLKTHDLVFASRPP E  KH+S+GQK+++F++YG 
Sbjct: 61  YGDIMYMRLGLMPTIVISSPEAAELFLKTHDLVFASRPPHEGSKHISFGQKNLIFSEYGA 120

Query: 121 YWRNMRKMFTLELLSSHKANSFKSMRREELSSLIDDLREAAKS-GAVVDLTSKVCALSAN 180
           YWR+ RKM T+ELLS+HK NSFKSMRREE+S  ++ +R AA + G  VDL+ KV +LS +
Sbjct: 121 YWRDTRKMCTIELLSNHKINSFKSMRREEVSLCVESIRAAANNRGVAVDLSDKVSSLSVD 180

Query: 181 ITCLMIFGRKFEDEELDERGFKWMTQEAMRLGGAFNLGDFVPFIAVLDVGGLNRRAKSVA 240
           ++C M+ G+K+ DEE DERGFK + +EA++L  A NLGD++ FIA LD+ G  +R KSV 
Sbjct: 181 MSCRMVLGKKYRDEEFDERGFKSVVREAIQLASAPNLGDYIRFIAPLDLQGFTKRMKSVN 240

Query: 241 KVFDRFFERIIDEHLQSSKNDATKDFVHVMLDIMGSKETEYQIDRSNIKALMLDLLLGAM 300
           K FD  FE+II+EHLQ +  + T DFV VM+  MGS+E+EY+I+R +IKA+MLD+L+ +M
Sbjct: 241 KAFDNLFEKIIEEHLQPNDGERTMDFVDVMVGFMGSEESEYRIERPHIKAIMLDMLVASM 300

Query: 301 DTSATTIGWIVAELIRHPRVMKKVQEELEKVVGLNRMVQETDLDHLEYLKMVVKEVFRLH 360
           DTSATTI W ++EL+RHP+ MKKVQ+ELE VVGL++MV+E+DL+ L+YL MVVKE FRLH
Sbjct: 301 DTSATTIEWALSELMRHPKAMKKVQKELENVVGLDKMVEESDLEKLDYLNMVVKETFRLH 360

Query: 361 PPGPLLAPHESLEDCIVNHFYIPK-------------------------------NHVDL 420
           P  PLL PH S+EDC VN ++IPK                               + VD+
Sbjct: 361 PVAPLLIPHASIEDCTVNGYHIPKKSRVLINVWAIGRDPNAWTDAEKFIPERFEGSSVDV 420

Query: 421 KGRDFQLIPFGSGRRGCPGIQMGLVVVHLVIAQLVHCFDWKLPNGMSPAESNMTEEFGLT 465
           +G  FQLIPFGSGRR CPGIQ+GL VV LV+AQLVHCFDW+LPN M P E +MTEEFGLT
Sbjct: 421 RGNHFQLIPFGSGRRRCPGIQLGLTVVQLVLAQLVHCFDWELPNNMLPEELDMTEEFGLT 480

[Close]

BLAST of Lag0034854 vs. ExPASy Swiss-Prot
Match: H2DH18 (Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 5.1e-132
Identity = 242/476 (50.84%), Postives = 320/476 (67.23%), Query Frame = 0


Query: 26  KNKKLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQKYGAIMHMKLGLVNTIVVSSPEAAEL 85
           ++ KLPPGP+G PI GSLH LG LPHR L  L++KYG IM M+LG V TIVVSSP+AAEL
Sbjct: 29  RHHKLPPGPRGLPIIGSLHTLGALPHRTLQTLAKKYGPIMSMRLGSVPTIVVSSPQAAEL 88

Query: 86  FLKTHDLVFASRPPSEAFKHMSYGQKSMVFAKYGPYWRNMRKMFTLELLSSHKANSFKSM 145
           FLKTHD +FASRP  +A ++MSYG   M F  YGP+WRN+RK   LELL+  K NSF  M
Sbjct: 89  FLKTHDNIFASRPKLQAAEYMSYGTMGMSFTAYGPHWRNIRKFVVLELLTPAKINSFVGM 148

Query: 146 RREELSSLIDDLREAAKSGAVVDLTSKVCALSANITCLMIFGRKFEDEELDERGFKWMTQ 205
           RREEL +++  ++EA+ +  VVDL++KV  +  N+T  ++ GR  +D+  D +G   +  
Sbjct: 149 RREELGTVVKSIKEASAANEVVDLSAKVANIIENMTYRLLLGRT-KDDRYDLKG---IMN 208

Query: 206 EAMRLGGAFNLGDFVPFIAVLDVGGLNRRAKSVAKVFDRFFERIIDEHLQSSKN-DATKD 265
           EA+ L G FN+ DFVPF+  LD+ GL R+ K   K  D+  E IIDEH Q+S N +A+ D
Sbjct: 209 EALTLAGRFNIADFVPFLGPLDIQGLTRQFKDTGKRLDKILEFIIDEHEQNSSNGNASGD 268

Query: 266 FVHVMLDIMGSKETEYQ-----IDRSNIKALMLDLLLGAMDTSATTIGWIVAELIRHPRV 325
           F+  ML +       +      IDRS IKA+M+D++  A+DTS T+I WI+ ELI+HPR 
Sbjct: 269 FIDDMLSLKNKPSNTHDELSKVIDRSVIKAIMIDIISAAIDTSDTSIEWILTELIKHPRA 328

Query: 326 MKKVQEELEKVVGLNRMVQETDLDHLEYLKMVVKEVFRLHPPGPLLAPHESLEDCIVNHF 385
           MKK QEE++ VVG++RMV+ETDL +LEY+ MVVKE  RLHP  PLL PHES+ED  +N +
Sbjct: 329 MKKCQEEIDAVVGVDRMVEETDLPNLEYVYMVVKEGLRLHPVAPLLGPHESMEDITINGY 388

Query: 386 YIPK--------------------------------NHVDLKGRDFQLIPFGSGRRGCPG 445
           +IPK                                ++VD++GRDFQL+PFGSGRRGCPG
Sbjct: 389 FIPKQSRVIVNSWALGRDPNVWSENAEEFLPERFEGSNVDVRGRDFQLLPFGSGRRGCPG 448

Query: 446 IQMGLVVVHLVIAQLVHCFDWKLPNGMSPAESNMTEEFGLTCSIAQDIMVTPIYRL 464
           +Q+GL+ V LV+A+LVHCFDW LPNG +P   +MTE+FGLT    + ++  P YRL
Sbjct: 449 MQLGLITVQLVVARLVHCFDWNLPNGTTPDNLDMTEKFGLTTPRVKHLLAVPKYRL 500

[Close]

BLAST of Lag0034854 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 353.2 bits (905), Expect = 4.5e-96
Identity = 188/484 (38.84%), Postives = 281/484 (58.06%), Query Frame = 0


Query: 7   IIIHLLALALVFGPWLL-----RNKNKKLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQKY 66
           I++ LL LA+    +LL     R K   LPP P   PI G+LH LG LPHR L  L+ + 
Sbjct: 3   ILVSLLFLAIALTFFLLKLNEKREKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLANEL 62

Query: 67  GAIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPPSEAFKHMSYGQKSMVFAKYGPY 126
           G ++ + LG + T++VS+ E AE  LKTHDL+FASRP + A + + Y    + F+ YG Y
Sbjct: 63  GPLILLHLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEY 122

Query: 127 WRNMRKMFTLELLSSHKANSFKSMRREELSSLIDDLREAAKSGAVVDLTSKVCALSANIT 186
           WR +RK+  LELLS  + NS++S+R EE+  +++ + ++  +G  V+L+  +  LS+   
Sbjct: 123 WRQVRKICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGTI 182

Query: 187 CLMIFGRKFEDEELDERGFKWMTQEAMRLGGAFNLGDFVPFIAVLDV-GGLNRRAKSVAK 246
             + FG+K+E EE  +  F  +  E   L GAF +GD+ P  A +DV  G++ R K    
Sbjct: 183 TRVAFGKKYEGEEERKNKFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHG 242

Query: 247 VFDRFFERIIDEHLQSSKNDAT-----KDFVHVMLDIMGSKETEYQIDRSNIKALMLDLL 306
             D F + +ID+HL S K + +     KD V V+L +         ++R+N+KA++LD+ 
Sbjct: 243 ELDAFVDHVIDDHLLSRKANGSDGVEQKDLVDVLLHLQKDSSLGVHLNRNNLKAVILDMF 302

Query: 307 LGAMDTSATTIGWIVAELIRHPRVMKKVQEELEKVVGLNRMVQETDLDHLEYLKMVVKEV 366
            G  DT+A T+ W +AELI+HP VM+K Q+E+ +VVG    V+E DL  L YLK+++KE 
Sbjct: 303 SGGTDTTAVTLEWAMAELIKHPDVMEKAQQEVRRVVGKKAKVEEEDLHQLHYLKLIIKET 362

Query: 367 FRLHPPGPLLAPHESLEDCIVNHFYIP-------------------------------KN 426
            RLHP  PLL P ES  D ++  ++IP                                N
Sbjct: 363 LRLHPVAPLLVPRESTRDVVIRGYHIPAKTRVFINAWAIGRDPKSWENAEEFLPERFVNN 422

Query: 427 HVDLKGRDFQLIPFGSGRRGCPGIQMGLVVVHLVIAQLVHCFDWKLPNGMSPAESNMTEE 449
            VD KG+DFQLIPFG+GRRGCPGI  G+  V + +A L++ F+W+LP  ++  + +M+E 
Sbjct: 423 SVDFKGQDFQLIPFGAGRRGCPGIAFGISSVEISLANLLYWFNWELPGDLTKEDLDMSEA 482

[Close]

BLAST of Lag0034854 vs. ExPASy Swiss-Prot
Match: A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 8.5e-95
Identity = 192/502 (38.25%), Postives = 298/502 (59.36%), Query Frame = 0


Query: 3   WIWIIIIHLLALALVFGPWLLRNKN-KKLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQKY 62
           ++WI  + LL+  +V+  +  R+     LPPGP G+PI G+L  LG  PH+ L  LS++Y
Sbjct: 6   FLWISTL-LLSSFIVYLLYRRRSAQCPPLPPGPNGWPILGNLPQLGAKPHQTLDALSKQY 65

Query: 63  GAIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPPSEAFKHMSYGQKSMVFAKYGPY 122
           G +  ++LG VN +V SS   A  FL+THD+ F++RPP+   +H++Y  + +VFA YGP 
Sbjct: 66  GPLFRLRLGSVNVVVASSSAVAAQFLRTHDVNFSNRPPNSGAEHVAYNYQDLVFAPYGPR 125

Query: 123 WRNMRKMFTLELLSSHKANSFKSMRREELSSLIDDLREAAKSGAVVDLTSKVCALSANIT 182
           WR +RK+ ++ L S    +  + +R+ E++ L+ +LR  A +G +V+L   +   + N  
Sbjct: 126 WRMLRKLCSVHLFSLKALDDLRPVRQGEVACLVRNLRRHADTGVLVNLGKALNVCATNAL 185

Query: 183 CLMIFGRKF---EDEELDERG-FKWMTQEAMRLGGAFNLGDFVPFIAVLDVGGLNRRAKS 242
              + GR+    ED +L E   FK M  E MRL G FN+GDFVP +  LD+ G+  + K 
Sbjct: 186 ARAMLGRRVFADEDAQLAEADEFKEMVVELMRLAGVFNVGDFVPGLGWLDLQGVVGKMKR 245

Query: 243 VAKVFDRFFERIIDEHLQSSKNDATKDFVHVMLDIM-GSKETEYQIDRSNIKALMLDLLL 302
           + + +D F +R+I+E+  ++K+    D + V++ +     E E +++ ++IKAL+L+L  
Sbjct: 246 LHRRYDAFLDRVIEENQANAKSG---DLLSVLIRLKEADAEGEIKLNNTDIKALLLNLFT 305

Query: 303 GAMDTSATTIGWIVAELIRHPRVMKKVQEELEKVVGLNRMVQETDLDHLEYLKMVVKEVF 362
              DTS++T+ W++AELIRHP +++K Q EL+ V+G +R+V E+DL +L YL+ VVKE F
Sbjct: 306 AGTDTSSSTVEWVLAELIRHPDILQKTQHELDSVIGRDRLVAESDLPNLPYLQAVVKETF 365

Query: 363 RLHPPGPLLAPHESLEDCIVNHFYIPKN-------------------------------- 422
           RLHP  PL  P  + E+CIVN + IPK+                                
Sbjct: 366 RLHPSTPLSLPRMASEECIVNGYKIPKHATLLVNVWSIGRDAAVWNDPLEFRPSRFLPGG 425

Query: 423 ---HVDLKGRDFQLIPFGSGRRGCPGIQMGLVVVHLVIAQLVHCFDWKLPNGMSPAESNM 464
              HVD+KG DF++IPFG+GRR C G+ +GL +V  + A +VH +DW LP G    + +M
Sbjct: 426 EREHVDVKGNDFEVIPFGAGRRICAGLSLGLRMVQFMTATIVHAYDWSLPKGQECQKLDM 485

[Close]

BLAST of Lag0034854 vs. ExPASy Swiss-Prot
Match: Q50EK4 (Cytochrome P450 750A1 OS=Pinus taeda OX=3352 GN=CYP750A1 PE=2 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 3.0e-92
Identity = 193/496 (38.91%), Postives = 280/496 (56.45%), Query Frame = 0


Query: 18  FGPWLLR--NKNKKLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQKYGAIMHMKLGLVNTI 77
           F  W+L    +N++LPPGP  +PI G+ H +    HR L  L++KYG I+ ++ G V T+
Sbjct: 28  FSCWILHQSQRNERLPPGPYPWPIIGNFHQVRLPLHRTLKNLAEKYGPILFLRFGSVPTV 87

Query: 78  VVSSPEAAELFLKTHDLVFASRPPSEAFKHMSYGQKSMVFAKYGPYWRNMRKMFTLELLS 137
           VVSS E A+ FLKTHDL+FASRPP+   K+  Y  K + F+ YG +WR MRK+  LELL+
Sbjct: 88  VVSSSEKAKHFLKTHDLIFASRPPTSVGKYFFYNFKDIAFSPYGDHWRKMRKICVLELLT 147

Query: 138 SHKANSFKSMRREELSSLIDDLREAAKSGAVVDLTSKVCALS-ANITCLMIFGRKFEDEE 197
           S +  SFK +R+EELS++I  + E ++SG +    SK  + S ANI   ++  +KF D +
Sbjct: 148 SKRIESFKHVRQEELSAMIHSIWEESESGRIAVNVSKAISTSLANILWRILARKKFSDND 207

Query: 198 L--DERGFKWMTQEAMRLGGAFNLGDFVPFIAVLDVGGLNRRAKSVAKVFDRFFERIIDE 257
           L  D +GF  +  E     G+ N+GDF+P++  LD+ G+ R  K     FD F E++IDE
Sbjct: 208 LGADGKGFADLVVEVSIAVGSLNIGDFIPYLDCLDLQGIKRALKKANARFDAFAEKMIDE 267

Query: 258 HLQSS-----KNDA---TKDFVHVMLDIMGSKETEYQIDRSNIKALMLDLLLGAMDTSAT 317
           H+ +S     + DA    KD + V+L++  +  T  ++ R  IKA+  +L    M+TSA 
Sbjct: 268 HINASTIRNGEADAGCHVKDIIDVLLEMAKNDNTGAKVTREIIKAITYELFSAGMETSAN 327

Query: 318 TIGWIVAELIRHPRVMKKVQEELEKVVGLNRMVQETDLDHLEYLKMVVKEVFRLHPPGPL 377
            + W ++EL+RHP  MKK+Q+E+E VVG    V+E+DL  + YL  VVKE  RL+P  PL
Sbjct: 328 VLEWAMSELLRHPHAMKKLQQEIESVVGQQGTVKESDLASIVYLHCVVKETLRLYPSLPL 387

Query: 378 LAPHESLEDCIVNHFYIPK-----------------------------------NHVDLK 437
             PHESLE   V  +YIPK                                   N +DL 
Sbjct: 388 ALPHESLEAVTVGGYYIPKKTMVIMNLWAIGRDPSVWGADASEFKPERFMQMEENGIDLS 447

Query: 438 G--RDFQLIPFGSGRRGCPGIQMGLVVVHLVIAQLVHCFDWKLPNGMSPAESNMTEEFGL 464
           G   DF+++PFG+GRR CPG  M ++ V   +AQL+H FDW++     P+E +M E    
Sbjct: 448 GGQSDFRMLPFGAGRRTCPGSAMAILTVEFTLAQLLHTFDWRVEG--DPSELDMKEACAT 507

[Close]

BLAST of Lag0034854 vs. ExPASy TrEMBL
Match: A0A6J1GET0 (cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453535 PE=3 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 6.1e-205
Identity = 357/496 (71.98%), Postives = 414/496 (83.47%), Query Frame = 0


Query: 1   MAWIWIIIIHLLALALVFGPWLLRNKNKKLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQK 60
           MAWIWIIII + AL L+F PWLL+NK K LPPGPKGFPI GSLHLLGKLPHRD+ RLSQ+
Sbjct: 1   MAWIWIIII-MFALVLLFWPWLLKNKGKNLPPGPKGFPILGSLHLLGKLPHRDIQRLSQR 60

Query: 61  YGAIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPPSEAFKHMSYGQKSMVFAKYGP 120
           YG IMHMKLGLVNTIVVSSPEAA LFLKTHDLVFASRP +E FKHMSYG++SMVF+KYG 
Sbjct: 61  YGPIMHMKLGLVNTIVVSSPEAAALFLKTHDLVFASRPTNEVFKHMSYGERSMVFSKYGH 120

Query: 121 YWRNMRKMFTLELLSSHKANSFKSMRREELSSLIDDLREAAKSGAVVDLTSKVCALSANI 180
           YWRNMRK+ TLELLS+ K N FKSMR+EELS LID+LREAAKS  VV+L+SKVC+LSAN+
Sbjct: 121 YWRNMRKICTLELLSNQKVNFFKSMRKEELSLLIDELREAAKSEVVVNLSSKVCSLSANM 180

Query: 181 TCLMIFGRKFEDEELDERGFKWMTQEAMRLGGAFNLGDFVPFIAVLDVGGLNRRAKSVAK 240
           TCLMIFG+KFED +LDERGFKW+ QEA++L  AFNLGDF+PFI+ LD+ GL +RAK V K
Sbjct: 181 TCLMIFGKKFEDRDLDERGFKWVVQEALKLAAAFNLGDFIPFISALDLQGLIQRAKCVNK 240

Query: 241 VFDRFFERIIDEHLQSSKNDATKDFVHVMLDIMGSKETEYQIDRSNIKALMLDLLLGAMD 300
           V DRFFERI+DEHL+ SKN+ +KDFV VML IMGS+ TEYQIDRS+IKA+MLD+LL A D
Sbjct: 241 VCDRFFERILDEHLE-SKNEDSKDFVDVMLGIMGSEVTEYQIDRSSIKAIMLDILLAATD 300

Query: 301 TSATTIGWIVAELIRHPRVMKKVQEELEKVVGLNRMVQETDLDHLEYLKMVVKEVFRLHP 360
           +SA TIGW VAELI+HP VMKKVQ+ELEKVVGLNRMV+ETDL HL YL+MVVKEV RLHP
Sbjct: 301 SSAVTIGWAVAELIKHPHVMKKVQDELEKVVGLNRMVEETDLSHLTYLEMVVKEVLRLHP 360

Query: 361 PGPLLAPHESLEDCIVNHFYIPK-------------------------------NHVDLK 420
           PGPL+ PHE+++DC VN+F+IPK                               + VD+K
Sbjct: 361 PGPLIVPHEAVKDCTVNNFHIPKKSRIIINVWAIGRNPSAWTDAEKFFPERFIGSQVDVK 420

Query: 421 GRDFQLIPFGSGRRGCPGIQMGLVVVHLVIAQLVHCFDWKLPNGMSPAESNMTEEFGLTC 466
           G+DFQL+PFGSGRRGCPG+Q+GLV +HL++AQLVHCFDWKLPNGM PA+ +MTE FGL+C
Sbjct: 421 GKDFQLLPFGSGRRGCPGMQVGLVTIHLILAQLVHCFDWKLPNGMLPADLDMTEAFGLSC 480

[Close]

BLAST of Lag0034854 vs. ExPASy TrEMBL
Match: A0A6J1IU54 (cytochrome P450 CYP736A12-like OS=Cucurbita maxima OX=3661 GN=LOC111478446 PE=3 SV=1)

HSP 1 Score: 713.4 bits (1840), Expect = 6.3e-202
Identity = 354/496 (71.37%), Postives = 411/496 (82.86%), Query Frame = 0


Query: 1   MAWIWIIIIHLLALALVFGPWLLRNKNKKLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQK 60
           MAWIW+III + AL L+F  WLL+NK K LPPGPKGFPI GSLHLLGKLPHRD+ RLSQ+
Sbjct: 1   MAWIWMIII-MFALVLLFRSWLLKNKAKNLPPGPKGFPILGSLHLLGKLPHRDIQRLSQR 60

Query: 61  YGAIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPPSEAFKHMSYGQKSMVFAKYGP 120
           YG IMHMKLGLVNTIVVSSPEAA LFLKTHDLVFASRP ++ FKHMSYG++SMVF+KYG 
Sbjct: 61  YGPIMHMKLGLVNTIVVSSPEAAALFLKTHDLVFASRPQNQVFKHMSYGERSMVFSKYGH 120

Query: 121 YWRNMRKMFTLELLSSHKANSFKSMRREELSSLIDDLREAAKSGAVVDLTSKVCALSANI 180
           YWRNMRK+ TLELLS+ K N FKSMRREELS LID+LREAAKS  VV+L+SKVC+LSAN+
Sbjct: 121 YWRNMRKICTLELLSNQKVNFFKSMRREELSLLIDELREAAKSEVVVNLSSKVCSLSANM 180

Query: 181 TCLMIFGRKFEDEELDERGFKWMTQEAMRLGGAFNLGDFVPFIAVLDVGGLNRRAKSVAK 240
           TCLMIFG+KFED +LDERGFKW+ QEA++L  AFNLGDF+PFI+ LD+ GL +RAK V K
Sbjct: 181 TCLMIFGKKFEDRDLDERGFKWVVQEALKLAAAFNLGDFIPFISALDLQGLIQRAKCVNK 240

Query: 241 VFDRFFERIIDEHLQSSKNDATKDFVHVMLDIMGSKETEYQIDRSNIKALMLDLLLGAMD 300
           V DRFFERI+DEHL+ SKND +KDFV VML IMGS+ TEYQIDRS+IKA+MLD+LL A D
Sbjct: 241 VCDRFFERILDEHLE-SKNDDSKDFVDVMLGIMGSEVTEYQIDRSSIKAIMLDILLAATD 300

Query: 301 TSATTIGWIVAELIRHPRVMKKVQEELEKVVGLNRMVQETDLDHLEYLKMVVKEVFRLHP 360
           +SA TIGW VAELI+HP VMK VQ+ELEKVVGLNRMV+ETDL HL YL++VVKEV RLHP
Sbjct: 301 SSAVTIGWAVAELIKHPYVMKNVQDELEKVVGLNRMVEETDLCHLTYLEIVVKEVLRLHP 360

Query: 361 PGPLLAPHESLEDCIVNHFYIPK-------------------------------NHVDLK 420
           PGPL+ PHE+++DC VN+F+IPK                               + VD+K
Sbjct: 361 PGPLIVPHEAVKDCTVNNFHIPKKSRIIINVWAIGRNPSAWTDAEKFFPERFIGSQVDVK 420

Query: 421 GRDFQLIPFGSGRRGCPGIQMGLVVVHLVIAQLVHCFDWKLPNGMSPAESNMTEEFGLTC 466
           G+DFQL+PFGSGRRGCPGIQ+GLV VHL++AQL+HCFDWKLPNGM PA+ +M E FGL+C
Sbjct: 421 GKDFQLLPFGSGRRGCPGIQVGLVTVHLILAQLIHCFDWKLPNGMLPADLDMMEAFGLSC 480

[Close]

BLAST of Lag0034854 vs. ExPASy TrEMBL
Match: A0A6J1GEV6 (cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453536 PE=3 SV=1)

HSP 1 Score: 684.5 bits (1765), Expect = 3.1e-193
Identity = 346/497 (69.62%), Postives = 399/497 (80.28%), Query Frame = 0


Query: 1   MAWIWIIIIHLLALALVFGPWLLRNKNKKLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQK 60
           MAWIWIIII +  L L+F  WL RN+++KLPPGPKGFPI GSLHLLGKLPHRDLHRLSQ 
Sbjct: 1   MAWIWIIII-MFTLVLLFWAWLGRNQSQKLPPGPKGFPILGSLHLLGKLPHRDLHRLSQT 60

Query: 61  YGAIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPPSEAFKHMSYGQKSMVFAKYGP 120
           YG IMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASR P+E FKH+S+GQK+MVFAKYGP
Sbjct: 61  YGPIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRAPNEVFKHISFGQKTMVFAKYGP 120

Query: 121 YWRNMRKMFTLELLSSHKANSFKSMRREELSSLIDDLR-EAAKSGAVVDLTSKVCALSAN 180
           YWRNM KM  LELLS+HK +SFKSMRR+EL  LID LR +AA S  VV+LTSK+ +L+ N
Sbjct: 121 YWRNMHKMCVLELLSNHKIDSFKSMRRQELGVLIDQLRDDAANSRVVVNLTSKIYSLTIN 180

Query: 181 ITCLMIFGRKFEDEELDERGFKWMTQEAMRLGGAFNLGDFVPFIAVLDVGGLNRRAKSVA 240
           + CLM+ GRKFED+E+DERGFKWM QE   L GAFNLGDF+PFIA LD+ G   RA+SV 
Sbjct: 181 MACLMVLGRKFEDKEIDERGFKWMIQEIFHLAGAFNLGDFIPFIAPLDLQGFTPRARSVH 240

Query: 241 KVFDRFFERIIDEHLQSSKNDATKDFVHVMLDIMGSKETEYQIDRSNIKALMLDLLLGAM 300
           KV D  FE+I+DEHL+S+ N  TKDFV VML IMGS++     DRSNIKA+MLD+LLGA+
Sbjct: 241 KVCDTLFEKILDEHLESNHNH-TKDFVDVMLGIMGSQQ-----DRSNIKAIMLDMLLGAV 300

Query: 301 DTSATTIGWIVAELIRHPRVMKKVQEELEKVVGLNRMVQETDLDHLEYLKMVVKEVFRLH 360
           DT+ T+IGW +A LI HP+VMKKVQ+ELEKVVGLNRMVQE DLDHLEYL+M VKEV RLH
Sbjct: 301 DTATTSIGWALAHLITHPQVMKKVQDELEKVVGLNRMVQEEDLDHLEYLEMTVKEVLRLH 360

Query: 361 PPGPLLAPHESLEDCIVNHFYIPK-------------------------------NHVDL 420
           PP PLL PHES++DCIVN+FYIPK                               + V++
Sbjct: 361 PPIPLLIPHESVKDCIVNNFYIPKRSRMIVNVWTIGQDPSVWNDPERFFLERFMGSKVEM 420

Query: 421 KGRDFQLIPFGSGRRGCPGIQMGLVVVHLVIAQLVHCFDWKLPNGMSPAESNMTEEFGLT 466
           KGRDFQLIPFGSGRRGCPG+Q+GL+ VHLV+AQLVHCFDWKLPNG  P E +MTE+FGLT
Sbjct: 421 KGRDFQLIPFGSGRRGCPGMQLGLIAVHLVLAQLVHCFDWKLPNGKLPFELDMTEKFGLT 480

[Close]

BLAST of Lag0034854 vs. ExPASy TrEMBL
Match: A0A6J1GET5 (cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453534 PE=3 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 2.1e-189
Identity = 336/496 (67.74%), Postives = 390/496 (78.63%), Query Frame = 0


Query: 1   MAWIWIIIIHLLALALVFGPWLLRNKNKKLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQK 60
           MAWIW+III + AL L+F  W+ RNK+KKLPPGPKGFPI GSLHLLGKLPHRDLHRLSQ 
Sbjct: 1   MAWIWMIII-MFALVLLFWAWMERNKSKKLPPGPKGFPIMGSLHLLGKLPHRDLHRLSQT 60

Query: 61  YGAIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPPSEAFKHMSYGQKSMVFAKYGP 120
           YG IMHMKLGLVNTIVVSSPEAAELFLKTHD+VFASR P+E FKH+S+ QK++ FAKYGP
Sbjct: 61  YGPIMHMKLGLVNTIVVSSPEAAELFLKTHDVVFASRAPNEVFKHVSFSQKTIAFAKYGP 120

Query: 121 YWRNMRKMFTLELLSSHKANSFKSMRREELSSLIDDLREAAKSGAVVDLTSKVCALSANI 180
           YWRNMRKM  LELLS+HKAN  +SMRR+EL   I  LR+AA SG VV+++SK+ +L+AN+
Sbjct: 121 YWRNMRKMCILELLSNHKANYLQSMRRQELGFTIHQLRDAANSGMVVNISSKIYSLTANM 180

Query: 181 TCLMIFGRKFEDEELDERGFKWMTQEAMRLGGAFNLGDFVPFIAVLDVGGLNRRAKSVAK 240
            CLMI GR+FED+ELDE GFKWM QE   L GAFNLGDF+PFIA LD+ G  R A+SV K
Sbjct: 181 ACLMILGRRFEDKELDEMGFKWMIQEIFHLAGAFNLGDFIPFIAPLDLQGFIRGARSVHK 240

Query: 241 VFDRFFERIIDEHLQSSKNDATKDFVHVMLDIMGSKETEYQIDRSNIKALMLDLLLGAMD 300
           V D FFE+I+DEHL+S+ ND TKD V VML IMGS+ TEYQID S IKA++LD+LLG ++
Sbjct: 241 VCDTFFEKILDEHLESNNND-TKDLVDVMLGIMGSQTTEYQIDPSTIKAIILDMLLGGVE 300

Query: 301 TSATTIGWIVAELIRHPRVMKKVQEELEKVVGLNRMVQETDLDHLEYLKMVVKEVFRLHP 360
           T ATTI W +AELIRHP+ MKKVQ+EL+K+VGLNRMVQE DLDHLEYL MVVKE FRLHP
Sbjct: 301 TPATTIEWALAELIRHPQTMKKVQDELQKIVGLNRMVQEADLDHLEYLDMVVKETFRLHP 360

Query: 361 PGPLLAPHESLEDCIVNHFYIPK-------------------------------NHVDLK 420
           P PL  P ES+ED +V  FYIPK                               N VD+K
Sbjct: 361 PSPLPIPRESVEDSVVKDFYIPKKSRIIINVWAIGRDPSVWIDAEKFFPERFIGNQVDVK 420

Query: 421 GRDFQLIPFGSGRRGCPGIQMGLVVVHLVIAQLVHCFDWKLPNGMSPAESNMTEEFGLTC 466
           GRDFQL+PFGSGRRGCPGIQMGLV VHLV+AQLVHCFDWKL N M P + +MTE+FGL+C
Sbjct: 421 GRDFQLLPFGSGRRGCPGIQMGLVTVHLVLAQLVHCFDWKLLNSMVPDKLDMTEKFGLSC 480

[Close]

BLAST of Lag0034854 vs. ExPASy TrEMBL
Match: A0A6J1BYU0 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005927 PE=3 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 1.7e-186
Identity = 328/497 (66.00%), Postives = 400/497 (80.48%), Query Frame = 0


Query: 1   MAWIWIIIIHLLALA-LVFGPWLLRNKNKKLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQ 60
           MAWI ++ + L  LA L+  PWL R++N KLPPGP+GFP+FGSLHLLGKLPHRDL  LSQ
Sbjct: 1   MAWI-LLAVALAGLALLILKPWLFRSQN-KLPPGPRGFPVFGSLHLLGKLPHRDLRDLSQ 60

Query: 61  KYGAIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPPSEAFKHMSYGQKSMVFAKYG 120
           KYG+IM+M+LG+V TI+V+SP+AAELFLKTHDLVFASRP  EA K+MSYGQK++ FA+YG
Sbjct: 61  KYGSIMYMRLGIVPTIIVTSPQAAELFLKTHDLVFASRPFFEASKYMSYGQKNLGFAQYG 120

Query: 121 PYWRNMRKMFTLELLSSHKANSFKSMRREELSSLIDDLREAAKSGAVVDLTSKVCALSAN 180
            YWRNMRKM TLELLS HK NSF+SMRREE+  L+  LR+AAK   V++L+SK+ +L+ +
Sbjct: 121 SYWRNMRKMCTLELLSHHKVNSFRSMRREEVGLLVHYLRDAAKDHVVINLSSKIASLNTD 180

Query: 181 ITCLMIFGRKFEDEELDERGFKWMTQEAMRLGGAFNLGDFVPFIAVLDVGGLNRRAKSVA 240
           +TCLM+FG+K+ D E DERGFK + QEAM+L    NLGDF+P IA LD+ GLNRR+K+++
Sbjct: 181 MTCLMVFGKKYADAEFDERGFKSVIQEAMQLVATPNLGDFIPCIAALDLQGLNRRSKAIS 240

Query: 241 KVFDRFFERIIDEHLQSSKNDATKDFVHVMLDIMGSKETEYQIDRSNIKALMLDLLLGAM 300
           K+FD FFE+IIDEHL+S   + TKDFV VMLDIMGS+ETEYQIDRS+IKA++LD+L+ AM
Sbjct: 241 KIFDGFFEKIIDEHLESKNENKTKDFVDVMLDIMGSQETEYQIDRSSIKAIILDMLVAAM 300

Query: 301 DTSATTIGWIVAELIRHPRVMKKVQEELEKVVGLNRMVQETDLDHLEYLKMVVKEVFRLH 360
           DTSATT+GW +AELI+HP+VMKK+QEEL+KVVGL+R V+E+DLDHLEYL M VKE+ R+H
Sbjct: 301 DTSATTVGWAIAELIKHPQVMKKLQEELQKVVGLDRRVEESDLDHLEYLDMFVKEILRVH 360

Query: 361 PPGPLLAPHESLEDCIVNHFYIPK-------------------------------NHVDL 420
           P  PLL PHESLEDC VN ++IPK                               + VD+
Sbjct: 361 PAAPLLVPHESLEDCTVNGYHIPKKSRIIVNAWAIGQDPNSWVDPEKFFPERFIGSQVDV 420

Query: 421 KGRDFQLIPFGSGRRGCPGIQMGLVVVHLVIAQLVHCFDWKLPNGMSPAESNMTEEFGLT 466
           KGRDFQLIPFGSGRRGCPG+ MGLVVVH VIAQLVHCFDWKLPNGM PAE +MTEEFGLT
Sbjct: 421 KGRDFQLIPFGSGRRGCPGMHMGLVVVHFVIAQLVHCFDWKLPNGMLPAELDMTEEFGLT 480

[Close]

BLAST of Lag0034854 vs. TAIR 10
Match: AT5G57260.1 (cytochrome P450, family 71, subfamily B, polypeptide 10 )

HSP 1 Score: 335.1 bits (858), Expect = 9.0e-92
Identity = 180/503 (35.79%), Postives = 292/503 (58.05%), Query Frame = 0


Query: 1   MAWIWIIIIHLLALALVFGPWLLRNKNKKLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQK 60
           M  +W + + LL   L+      + +  + PP P G PI G+LH LG+LPH+ L +LS+K
Sbjct: 1   MTVLWFVSLILLISILLVAVKHSKRRWVRQPPSPPGLPIIGNLHQLGELPHQSLCKLSKK 60

Query: 61  YGAIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPPSEAFKHMSYGQKSMVFAKYGP 120
           YG +M +KLG V T++VS+PE A+  LK +DL   SRP  E  + +SY    + F+++  
Sbjct: 61  YGPVMLLKLGRVPTVIVSTPETAKQVLKDYDLHCCSRPSLEGTRKLSYNYLDIAFSRFDD 120

Query: 121 YWRNMRKMFTLELLSSHKANSFKSMRREELSSLIDDLREAAKSGAVVDLTSKVCALSANI 180
           YW+ +RK+   EL  + + NS + ++  E+  LID + E+A    +V+L+    +L+ N+
Sbjct: 121 YWKELRKLCVEELFCNKRINSIQPIKEAEMEKLIDSIAESASQKTLVNLSDTFLSLNVNV 180

Query: 181 TCLMIFGRKFEDEELDERGFKWMTQEAMRLGGAFNLGDFVPFIA-VLD-VGGLNRRAKSV 240
            C  +FG  F+   L+   F+ +  EA+ + G+F+  DF P++  ++D   GL+ R +  
Sbjct: 181 ICKAVFGVNFQGTVLNNDKFQDLVHEALEMLGSFSASDFFPYVGWIVDWFTGLHARRERS 240

Query: 241 AKVFDRFFERIIDEHLQSSKNDATKDFVHVMLDIMGSKETEY----QIDRSNIKALMLDL 300
            +  D F+E++ID HLQ ++ ++  DFV ++L +   KE       ++ R++IKA+++++
Sbjct: 241 VRDLDAFYEQMIDLHLQKNREESEDDFVDLLLRL--EKEEAVLGYGKLTRNHIKAILMNI 300

Query: 301 LLGAMDTSATTIGWIVAELIRHPRVMKKVQEELEKVVGLN---RMVQETDLDHLEYLKMV 360
           LLG ++TSA T+ W +AELIR+PRVMKKVQ E+   +G N   R++   +++HL YL MV
Sbjct: 301 LLGGINTSAITMTWAMAELIRNPRVMKKVQSEIRAQIGKNNKTRIISLDEINHLSYLNMV 360

Query: 361 VKEVFRLHPPGPLLAPHESLEDCIVNHFYI-PKNH------------------------- 420
           +KE  RLHP  PLL P E + +  +N + I PK                           
Sbjct: 361 IKETCRLHPVAPLLVPREVISEFKINGYTIQPKTRLHVNVWAIGRDPEIWKDPEEFLPER 420

Query: 421 -----VDLKGRDFQLIPFGSGRRGCPGIQMGLVVVHLVIAQLVHCFDWKLPNGMSPAESN 464
                +D+KG+D++L+PFGSGRR CP + MG+  V   +A L++ FDWKLP G++  +  
Sbjct: 421 FMDCDIDVKGQDYELLPFGSGRRICPAVYMGITTVEFGLANLLYHFDWKLPEGVAVEDIY 480

[Close]

BLAST of Lag0034854 vs. TAIR 10
Match: AT5G07990.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 332.0 bits (850), Expect = 7.6e-91
Identity = 191/502 (38.05%), Postives = 278/502 (55.38%), Query Frame = 0


Query: 3   WIWIIIIHLLALAL-VFGPWLLRNKNKKLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQKY 62
           ++ I++  +L L L +F     R+ N +LPPGP  +PI G+L  +G  PHR L  +   Y
Sbjct: 5   FLTILLATVLFLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKPHRTLSAMVTTY 64

Query: 63  GAIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPPSEAFKHMSYGQKSMVFAKYGPY 122
           G I+H++LG V+ +V +S   AE FLK HD  FASRPP+   KHM+Y  + +VFA YG  
Sbjct: 65  GPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYGHR 124

Query: 123 WRNMRKMFTLELLSSHKANSFKSMRREELSSLIDDLREAAKSGA-VVDLTSKVCALSANI 182
           WR +RK+ ++ L S+     FK +R+EE+ +L    RE  + G   V+L   V     N 
Sbjct: 125 WRLLRKISSVHLFSAKALEDFKHVRQEEVGTL---TRELVRVGTKPVNLGQLVNMCVVNA 184

Query: 183 TCLMIFGRKFEDEELDERG--FKWMTQEAMRLGGAFNLGDFVPFIAVLDVGGLNRRAKSV 242
               + GR+    + D +   F+ M  E M L G FN+GDFVP +  LD+ G+  + K +
Sbjct: 185 LGREMIGRRLFGADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKRL 244

Query: 243 AKVFDRFFERIIDEHLQSSKNDATKDFVHVMLDIMGS--KETEYQIDRSNIKALMLDLLL 302
            K FD F   I+ EH  + ++    D +  ++ + G+        +  + IKAL+L++  
Sbjct: 245 HKRFDAFLSSILKEHEMNGQDQKHTDMLSTLISLKGTDLDGDGGSLTDTEIKALLLNMFT 304

Query: 303 GAMDTSATTIGWIVAELIRHPRVMKKVQEELEKVVGLNRMVQETDLDHLEYLKMVVKEVF 362
              DTSA+T+ W +AELIRHP +M K QEEL+ VVG +R V E+D+  L YL+ V+KE F
Sbjct: 305 AGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVIKENF 364

Query: 363 RLHPPGPLLAPHESLEDCIVNHFYIP---------------------------------- 422
           RLHPP PL  PH + E C +N ++IP                                  
Sbjct: 365 RLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERFLPGG 424

Query: 423 -KNHVDLKGRDFQLIPFGSGRRGCPGIQMGLVVVHLVIAQLVHCFDWKLPNGMSPAESNM 464
            K+ VD+KG DF+LIPFG+GRR C G+ +GL  +  + A LV  FDW+L  G++P + NM
Sbjct: 425 EKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPEKLNM 484

[Close]

BLAST of Lag0034854 vs. TAIR 10
Match: AT4G36220.1 (ferulic acid 5-hydroxylase 1 )

HSP 1 Score: 330.1 bits (845), Expect = 2.9e-90
Identity = 180/508 (35.43%), Postives = 285/508 (56.10%), Query Frame = 0


Query: 6   IIIIHLLALALVFGPWLLRNKNKKLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQKYGAIM 65
           +I++ L     +F  ++ R +    PPGP+G+PI G++ ++ +L HR L  L++KYG + 
Sbjct: 20  VIVVSL----FIFISFITRRRRPPYPPGPRGWPIIGNMLMMDQLTHRGLANLAKKYGGLC 79

Query: 66  HMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPPSEAFKHMSYGQKSMVFAKYGPYWRNM 125
           H+++G ++   VSSPE A   L+  D VF++RP + A  +++Y +  M FA YGP+WR M
Sbjct: 80  HLRMGFLHMYAVSSPEVARQVLQVQDSVFSNRPATIAISYLTYDRADMAFAHYGPFWRQM 139

Query: 126 RKMFTLELLSSHKANSFKSMRREELSSLIDDLREAAKSGAVVDLTSKVCALSANITCLMI 185
           RK+  +++ S  +A S+ S+ R+E+  ++  +  +   G  +++  ++ AL+ NIT    
Sbjct: 140 RKVCVMKVFSRKRAESWASV-RDEVDKMVRSV--SCNVGKPINVGEQIFALTRNITYRAA 199

Query: 186 FGRKFEDEELDERGFKWMTQEAMRLGGAFNLGDFVPFIAVLDVGGLNRRAKSVAKVFDRF 245
           FG   E  + DE  F  + QE  +L GAFN+ DF+P+   +D  G+N+R        D F
Sbjct: 200 FGSACEKGQ-DE--FIRILQEFSKLFGAFNVADFIPYFGWIDPQGINKRLVKARNDLDGF 259

Query: 246 FERIIDEHLQSSKNDATKDFVHV----MLDIM--------------GSKETEYQIDRSNI 305
            + IIDEH++  +N    D   V    M+D +                 +   ++ R NI
Sbjct: 260 IDDIIDEHMKKKENQNAVDDGDVVDTDMVDDLLAFYSEEAKLVSETADLQNSIKLTRDNI 319

Query: 306 KALMLDLLLGAMDTSATTIGWIVAELIRHPRVMKKVQEELEKVVGLNRMVQETDLDHLEY 365
           KA+++D++ G  +T A+ I W + EL+R P  +K+VQ+EL +VVGL+R V+E+D++ L Y
Sbjct: 320 KAIIMDVMFGGTETVASAIEWALTELLRSPEDLKRVQQELAEVVGLDRRVEESDIEKLTY 379

Query: 366 LKMVVKEVFRLHPPGPLLAPHESLEDCIVNHFYIPKNH---------------------- 425
           LK  +KE  R+HPP PLL  HE+ ED  ++ F+IPK                        
Sbjct: 380 LKCTLKETLRMHPPIPLLL-HETAEDTSIDGFFIPKKSRVMINAFAIGRDPTSWTDPDTF 439

Query: 426 ----------VDLKGRDFQLIPFGSGRRGCPGIQMGLVVVHLVIAQLVHCFDWKLPNGMS 464
                      D KG +F+ IPFGSGRR CPG+Q+GL  + L +A ++HCF WKLP+GM 
Sbjct: 440 RPSRFLEPGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHILHCFTWKLPDGMK 499

[Close]

BLAST of Lag0034854 vs. TAIR 10
Match: AT3G26300.1 (cytochrome P450, family 71, subfamily B, polypeptide 34 )

HSP 1 Score: 327.4 bits (838), Expect = 1.9e-89
Identity = 179/492 (36.38%), Postives = 279/492 (56.71%), Query Frame = 0


Query: 3   WIWIIIIHLLALALVFGPWLLRNKNKKLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQKYG 62
           W+  +I  +  L  VF     RN  ++ PP P G PI G+LH LG+LPH+ L +LS+KYG
Sbjct: 5   WLLSLIFVICILVAVFNHKNRRN-YQRTPPSPPGCPIIGNLHQLGELPHQSLWKLSKKYG 64

Query: 63  AIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPPSEAFKHMSYGQKSMVFAKYGPYW 122
            +M +KLG V T++VSS E A+  LK HDL   SRP     + +SY    + F+ Y  YW
Sbjct: 65  PVMLLKLGRVPTVIVSSSETAKQALKIHDLHCCSRPGFAGARELSYNYLDIAFSPYDDYW 124

Query: 123 RNMRKMFTLELLSSHKANSFKSMRREELSSLIDDLREAAKSGAVVDLTSKVCALSANITC 182
           + +RK+   EL SS + +S + ++ EE+  LID + E+A     ++L   + AL+ ++ C
Sbjct: 125 KEVRKLAVQELFSSKQVHSIQPIKDEEVKKLIDSISESAAQKTPINLNKTLLALTVSVVC 184

Query: 183 LMIFGRKFEDEELDERGFKWMTQEAMRLGGAFNLGDFVPFIA-VLD-VGGLNRRAKSVAK 242
              F   FE   L+   F  + +EA+ + G+F+  DF+P++  ++D + GL  R +   +
Sbjct: 185 RTAFSVNFEGTVLNSERFNNIVREALEMLGSFSASDFIPYVGRIIDLLTGLQGRRERSMR 244

Query: 243 VFDRFFERIIDEHLQSSKNDATKDFVHVMLDIMGSKET--EYQIDRSNIKALMLDLLLGA 302
             D F+E++ D H Q  K + ++DFV ++L +   +      ++ R++IKA+++D+LL  
Sbjct: 245 DLDAFYEQMFDLHKQ-KKEEGSEDFVDLLLRLEKEEAVLGNDKLTRNHIKAILMDVLLAG 304

Query: 303 MDTSATTIGWIVAELIRHPRVMKKVQEELEKVVGLNRMVQETDLDHLEYLKMVVKEVFRL 362
           MDTSA T+ W +AEL ++PRVMKKVQ E+   +     +   D D LEYLKMV+KE +RL
Sbjct: 305 MDTSAITMTWAMAELAKNPRVMKKVQSEIRSQIKNKERISFDDTDKLEYLKMVIKETWRL 364

Query: 363 HPPGPLLAPHESLEDCIVNHFYIP-------------------------------KNHVD 422
           HP  PLL P E++ +  +N + IP                                N++D
Sbjct: 365 HPTTPLLIPREAMSEFEINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERFTDNNID 424

Query: 423 LKGRDFQLIPFGSGRRGCPGIQMGLVVVHLVIAQLVHCFDWKLPNGMSPAESNMTEEFGL 460
            KG+ F+L+PFG GRR CP + MG  +V   +A L++ FDWKLP GM   + +M E  GL
Sbjct: 425 AKGQHFELLPFGGGRRMCPAVYMGTTMVEFGLANLLYHFDWKLPEGMKVDDIDMEEAPGL 484

[Close]

BLAST of Lag0034854 vs. TAIR 10
Match: AT3G26310.1 (cytochrome P450, family 71, subfamily B, polypeptide 35 )

HSP 1 Score: 324.7 bits (831), Expect = 1.2e-88
Identity = 179/498 (35.94%), Postives = 284/498 (57.03%), Query Frame = 0


Query: 1   MAWIWII-IIHLLALALVFGPWLLRNKNKKLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQ 60
           MA IW++ +I L+ + L         K ++  P P GFPI G+LH +G+LPH+ L +LS+
Sbjct: 1   MAHIWLLPLIFLVCILLAVFNHKKHPKYRQF-PCPPGFPIIGNLHQIGELPHQTLWKLSK 60

Query: 61  KYGAIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPPSEAFKHMSYGQKSMVFAKYG 120
           KYG +MH+ LG V T+VVSS + A   L+ HDL   +RP     + +SY    + F+ Y 
Sbjct: 61  KYGPVMHLMLGRVPTVVVSSSDTARQVLRVHDLHCCTRPSLSGPRELSYNYLDIAFSPYD 120

Query: 121 PYWRNMRKMFTLELLSSHKANSFKSMRREELSSLIDDLREAAKSGAVVDLTSKVCALSAN 180
            YW+ +RK+   EL S+ + +S + ++ EE+  +ID + E+A     V+L +K   L+ +
Sbjct: 121 DYWKEVRKLCVQELFSTKQVHSIQPIKDEEVKKMIDSIAESASQKNPVNLNNKCLELTVS 180

Query: 181 ITCLMIFGRKFEDEELDERGFKWMTQEAMRLGGAFNLGDFVPFIA-VLDV-GGLNRRAKS 240
           + C   FG  FE   L+   F  + +EA+ + G+F+  DF+P++  ++DV  GL  R + 
Sbjct: 181 VVCRTAFGVSFEGTVLNSDRFNKIVREALEMLGSFSAADFIPYVGWIIDVLTGLQGRRER 240

Query: 241 VAKVFDRFFERIIDEHLQSSKNDATKDFVHVMLDIMGSKET--EYQIDRSNIKALMLDLL 300
             +  + FFE++ D H +  K +  +DFV ++L +   +      ++ R++IKA++LD+L
Sbjct: 241 SKRDLNAFFEQMFDLH-KEGKKEGNEDFVDLLLRLEKEEAVLGNDKLTRNHIKAILLDVL 300

Query: 301 LGAMDTSATTIGWIVAELIRHPRVMKKVQEELEKVVGLNRMVQETDLDHLEYLKMVVKEV 360
           L  +DTSA T+ W + EL R+PRVMKKVQ E+   +G   M+   D+D LEYLKMV+KE 
Sbjct: 301 LAGIDTSAITMTWAMTELARNPRVMKKVQSEIRTQMGNRSMISFEDMDQLEYLKMVIKET 360

Query: 361 FRLHPPGPLLAPHESLEDCIVNHFYIP-------------------------------KN 420
           +RLHP  PLL P E++ +  +N + IP                                N
Sbjct: 361 WRLHPTTPLLLPREAMSEFDINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERFMDN 420

Query: 421 HVDLKGRDFQLIPFGSGRRGCPGIQMGLVVVHLVIAQLVHCFDWKLPNGMSPAESNMTEE 463
           ++D KG+ F+L+PFG GRR CP I MG  +V   +A L++ FDWKLP G+   + ++ E 
Sbjct: 421 NIDAKGQHFELLPFGGGRRICPAIYMGTTMVEFGLANLLYHFDWKLPEGVEVKDIDVEEA 480

The following BLAST results are available for this feature:

BLAST of Lag0034854 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022950432.1	1.3e-204	71.98	cytochrome P450 CYP736A12-like [Cucurbita moschata]	[more]
XP_023545010.1	6.3e-204	71.98	cytochrome P450 CYP736A12-like [Cucurbita pepo subsp. pepo]	[more]
XP_022978479.1	1.3e-201	71.37	cytochrome P450 CYP736A12-like [Cucurbita maxima]	[more]
XP_023544864.1	5.0e-193	68.15	cytochrome P450 CYP736A12-like [Cucurbita pepo subsp. pepo]	[more]
XP_022950433.1	6.5e-193	69.62	cytochrome P450 CYP736A12-like [Cucurbita moschata]	[more]

BLAST of Lag0034854 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A068Q5V6	5.4e-166	58.47	Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1	[more]
H2DH18	5.1e-132	50.84	Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1	[more]
P24465	4.5e-96	38.84	Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2	[more]
A0A4D6Q415	8.5e-95	38.25	Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN...	[more]
Q50EK4	3.0e-92	38.91	Cytochrome P450 750A1 OS=Pinus taeda OX=3352 GN=CYP750A1 PE=2 SV=1	[more]

BLAST of Lag0034854 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GET0	6.1e-205	71.98	cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453535 PE=...	[more]
A0A6J1IU54	6.3e-202	71.37	cytochrome P450 CYP736A12-like OS=Cucurbita maxima OX=3661 GN=LOC111478446 PE=3 ...	[more]
A0A6J1GEV6	3.1e-193	69.62	cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453536 PE=...	[more]
A0A6J1GET5	2.1e-189	67.74	cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453534 PE=...	[more]
A0A6J1BYU0	1.7e-186	66.00	cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005927 PE...	[more]

BLAST of Lag0034854 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G57260.1	9.0e-92	35.79	cytochrome P450, family 71, subfamily B, polypeptide 10	[more]
AT5G07990.1	7.6e-91	38.05	Cytochrome P450 superfamily protein	[more]
AT4G36220.1	2.9e-90	35.43	ferulic acid 5-hydroxylase 1	[more]
AT3G26300.1	1.9e-89	36.38	cytochrome P450, family 71, subfamily B, polypeptide 34	[more]
AT3G26310.1	1.2e-88	35.94	cytochrome P450, family 71, subfamily B, polypeptide 35	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 396..405 score: 57.4 coord: 297..314 score: 27.89 coord: 350..361 score: 39.77
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 390..447 e-value: 3.5E-8 score: 32.6 coord: 31..384 e-value: 7.6E-72 score: 242.5
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 286..303 score: 26.47 coord: 82..103 score: 25.04 coord: 176..194 score: 27.66 coord: 405..428 score: 24.67 coord: 58..77 score: 29.21 coord: 306..332 score: 37.3 coord: 395..405 score: 53.79
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 23..459 e-value: 2.0E-109 score: 368.3
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 31..462
None	No IPR available	PANTHER	PTHR47943:SF2	CYTOCHROME P450 FAMILY 71 PROTEIN	coord: 384..464 coord: 1..384
None	No IPR available	PANTHER	PTHR47943	CYTOCHROME P450 93A3-LIKE	coord: 384..464 coord: 1..384
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 398..407

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0034854.1	Lag0034854.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen