Lag0034734 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0034734
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr3: 10291165 .. 10291941 (+)
RNA-Seq ExpressionLag0034734
SyntenyLag0034734
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGTCTGGTGGTGAAGACACCCATTGGTGCTATCAGTGCAACCATTCATTCTGGCTTAATGGAGAAGATGCAGTTTGCCCGTACTGTAATGGTGGTTTCGTTGAAGAACTTAACGATGTACATGATGAAACCGTTCAAAATGACTTTAATTCAGGCACAGAAGATGATCTTTCAACCCAGGTGCCTCCAATTTTTGAAGCCATGTTTGCATTGATGGGGCGAAGAAGCCCTTATCCAAGATTTGGACTTCTTGAAGCTGTTGATGCTTTTACCAGAGAAAGAATGGCTGGAAGAAATTCTAACTTCGACGTTAGGAGGAGATCCAGTTCTATCCCAGGGCAGAATGTGGATCGTTTCAATACCTTTTGGTCATTCCATGACCATGTTACTGTTCCGCCCTACAGTAGCGTCACTCCAGAAGATAGATCTAGTCCACGTTCGGCACTGGAAATATTAGCTGCACAGCTTTCCTTGAATGAACACAGGGATCCGGTTCCAGCTCCTGCCTCTCACTCTTCTATAGAAGCAATGCCTACAATCAAGATCAACCAAATGCACCTTGGCACTGACTCTCATTGTCCAGTCTGTAAAGAAAAGTTTGAGCTGGGATCTGAAGCCAAAGAGATGCCATGTAATCATATTTATCACACTGATTGCATTCTTCCTTGGCTGGTCCAGCACAATACTTGCCCGGTTTGTCGACTCGAGCTGCCTCAGCAGGATAGCGGACACGATTGGGGGGGAACAGCAGCAGCAACAACAGTGGAGTCTTAA

mRNA sequence

ATGTCGTCTGGTGGTGAAGACACCCATTGGTGCTATCAGTGCAACCATTCATTCTGGCTTAATGGAGAAGATGCAGTTTGCCCGTACTGTAATGGTGGTTTCGTTGAAGAACTTAACGATGTACATGATGAAACCGTTCAAAATGACTTTAATTCAGGCACAGAAGATGATCTTTCAACCCAGGTGCCTCCAATTTTTGAAGCCATGTTTGCATTGATGGGGCGAAGAAGCCCTTATCCAAGATTTGGACTTCTTGAAGCTGTTGATGCTTTTACCAGAGAAAGAATGGCTGGAAGAAATTCTAACTTCGACGTTAGGAGGAGATCCAGTTCTATCCCAGGGCAGAATGTGGATCGTTTCAATACCTTTTGGTCATTCCATGACCATGTTACTGTTCCGCCCTACAGTAGCGTCACTCCAGAAGATAGATCTAGTCCACGTTCGGCACTGGAAATATTAGCTGCACAGCTTTCCTTGAATGAACACAGGGATCCGGTTCCAGCTCCTGCCTCTCACTCTTCTATAGAAGCAATGCCTACAATCAAGATCAACCAAATGCACCTTGGCACTGACTCTCATTGTCCAGTCTGTAAAGAAAAGTTTGAGCTGGGATCTGAAGCCAAAGAGATGCCATGTAATCATATTTATCACACTGATTGCATTCTTCCTTGGCTGGTCCAGCACAATACTTGCCCGGTTTGTCGACTCGAGCTGCCTCAGCAGGATAGCGGACACGATTGGGGGGGAACAGCAGCAGCAACAACAGTGGAGTCTTAA

Coding sequence (CDS)

ATGTCGTCTGGTGGTGAAGACACCCATTGGTGCTATCAGTGCAACCATTCATTCTGGCTTAATGGAGAAGATGCAGTTTGCCCGTACTGTAATGGTGGTTTCGTTGAAGAACTTAACGATGTACATGATGAAACCGTTCAAAATGACTTTAATTCAGGCACAGAAGATGATCTTTCAACCCAGGTGCCTCCAATTTTTGAAGCCATGTTTGCATTGATGGGGCGAAGAAGCCCTTATCCAAGATTTGGACTTCTTGAAGCTGTTGATGCTTTTACCAGAGAAAGAATGGCTGGAAGAAATTCTAACTTCGACGTTAGGAGGAGATCCAGTTCTATCCCAGGGCAGAATGTGGATCGTTTCAATACCTTTTGGTCATTCCATGACCATGTTACTGTTCCGCCCTACAGTAGCGTCACTCCAGAAGATAGATCTAGTCCACGTTCGGCACTGGAAATATTAGCTGCACAGCTTTCCTTGAATGAACACAGGGATCCGGTTCCAGCTCCTGCCTCTCACTCTTCTATAGAAGCAATGCCTACAATCAAGATCAACCAAATGCACCTTGGCACTGACTCTCATTGTCCAGTCTGTAAAGAAAAGTTTGAGCTGGGATCTGAAGCCAAAGAGATGCCATGTAATCATATTTATCACACTGATTGCATTCTTCCTTGGCTGGTCCAGCACAATACTTGCCCGGTTTGTCGACTCGAGCTGCCTCAGCAGGATAGCGGACACGATTGGGGGGGAACAGCAGCAGCAACAACAGTGGAGTCTTAA

Protein sequence

MSSGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLSTQVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDRFNTFWSFHDHVTVPPYSSVTPEDRSSPRSALEILAAQLSLNEHRDPVPAPASHSSIEAMPTIKINQMHLGTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPWLVQHNTCPVCRLELPQQDSGHDWGGTAAATTVES
Homology
BLAST of Lag0034734 vs. NCBI nr
Match: XP_022147727.1 (probable E3 ubiquitin-protein ligase RHC1A isoform X1 [Momordica charantia])

HSP 1 Score: 460.7 bits (1184), Expect = 8.4e-126
Identity = 209/256 (81.64%), Postives = 232/256 (90.62%), Query Frame = 0

Query: 1   MSSGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGT-EDDLS 60
           MSSGGEDTHWCYQCNHSFWL GED VCPYCNGGF+EELND+HD T+QND N GT E+D S
Sbjct: 57  MSSGGEDTHWCYQCNHSFWLGGEDIVCPYCNGGFIEELNDLHDGTIQNDTNLGTEEEDFS 116

Query: 61  TQVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDR 120
           +QVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRN NFDVRRRSSS+PGQN+D 
Sbjct: 117 SQVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNPNFDVRRRSSSVPGQNLDF 176

Query: 121 FNTFWSFHDHVTVPPYSSVTPEDRSSPRSALEILAAQLSLNEHRDPVPAPASHSSIEAMP 180
           FN+FWSFH+HV  P ++SV PE RSS  S L+ L AQ+++NE RDPVPAPASHSSIEAMP
Sbjct: 177 FNSFWSFHEHVPGPSFASVAPEGRSSQLSGLQALGAQITMNERRDPVPAPASHSSIEAMP 236

Query: 181 TIKINQMHLGTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPWLVQHNTCPVCRLELP 240
           TIKINQMHLGTDSHCPVCKEKFELGSEA+EMPCNHIYH+DCILPWLVQHNTCPVCRLELP
Sbjct: 237 TIKINQMHLGTDSHCPVCKEKFELGSEAREMPCNHIYHSDCILPWLVQHNTCPVCRLELP 296

Query: 241 QQDSGHDWGGTAAATT 256
           QQD+ H W G +++++
Sbjct: 297 QQDTRHAWSGGSSSSS 312

BLAST of Lag0034734 vs. NCBI nr
Match: XP_022147728.1 (probable E3 ubiquitin-protein ligase RHC1A isoform X2 [Momordica charantia])

HSP 1 Score: 460.7 bits (1184), Expect = 8.4e-126
Identity = 209/256 (81.64%), Postives = 232/256 (90.62%), Query Frame = 0

Query: 1   MSSGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGT-EDDLS 60
           MSSGGEDTHWCYQCNHSFWL GED VCPYCNGGF+EELND+HD T+QND N GT E+D S
Sbjct: 1   MSSGGEDTHWCYQCNHSFWLGGEDIVCPYCNGGFIEELNDLHDGTIQNDTNLGTEEEDFS 60

Query: 61  TQVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDR 120
           +QVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRN NFDVRRRSSS+PGQN+D 
Sbjct: 61  SQVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNPNFDVRRRSSSVPGQNLDF 120

Query: 121 FNTFWSFHDHVTVPPYSSVTPEDRSSPRSALEILAAQLSLNEHRDPVPAPASHSSIEAMP 180
           FN+FWSFH+HV  P ++SV PE RSS  S L+ L AQ+++NE RDPVPAPASHSSIEAMP
Sbjct: 121 FNSFWSFHEHVPGPSFASVAPEGRSSQLSGLQALGAQITMNERRDPVPAPASHSSIEAMP 180

Query: 181 TIKINQMHLGTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPWLVQHNTCPVCRLELP 240
           TIKINQMHLGTDSHCPVCKEKFELGSEA+EMPCNHIYH+DCILPWLVQHNTCPVCRLELP
Sbjct: 181 TIKINQMHLGTDSHCPVCKEKFELGSEAREMPCNHIYHSDCILPWLVQHNTCPVCRLELP 240

Query: 241 QQDSGHDWGGTAAATT 256
           QQD+ H W G +++++
Sbjct: 241 QQDTRHAWSGGSSSSS 256

BLAST of Lag0034734 vs. NCBI nr
Match: XP_008451039.1 (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008451040.1 PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008451041.1 PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >KAA0055692.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK09941.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa])

HSP 1 Score: 459.9 bits (1182), Expect = 1.4e-125
Identity = 208/255 (81.57%), Postives = 227/255 (89.02%), Query Frame = 0

Query: 1   MSSGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLST 60
           MSS GEDTHWCYQCNHSFWL+GED VCP+CNGGFVEELND HDET+QNDFN G E+DLST
Sbjct: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLST 60

Query: 61  QVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDRF 120
           QVPPIFEAMFALMGRR+PYPRFGLLEAVD FTRERMAGRN NFDVRRRS S+PGQN+D F
Sbjct: 61  QVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120

Query: 121 NTFWSFHDHVTVPPYSSVTPEDRSSPRSALEILAAQLSLNEHRDPVPAPASHSSIEAMPT 180
           N+FWSFHDH++   + +VTPE RSS  + LE LAAQLSLNE R+PVP PASHS IEAMPT
Sbjct: 121 NSFWSFHDHMSGSTFGNVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 180

Query: 181 IKINQMHLGTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240
           IKINQMHLG+DSHCPVCKEKFEL SEAK +PCNHIYHTDCILPWLVQHNTCPVCRLELPQ
Sbjct: 181 IKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240

Query: 241 QDSGHDWGGTAAATT 256
           Q+SGH WGG   + +
Sbjct: 241 QESGHSWGGNGGSNS 255

BLAST of Lag0034734 vs. NCBI nr
Match: XP_004144098.1 (E3 ubiquitin-protein ligase RZF1 [Cucumis sativus] >XP_011660068.1 E3 ubiquitin-protein ligase RZF1 [Cucumis sativus] >XP_031736051.1 E3 ubiquitin-protein ligase RZF1 [Cucumis sativus] >KAE8653480.1 hypothetical protein Csa_007077 [Cucumis sativus])

HSP 1 Score: 454.5 bits (1168), Expect = 6.0e-124
Identity = 207/250 (82.80%), Postives = 225/250 (90.00%), Query Frame = 0

Query: 1   MSSGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLST 60
           MSS GEDTHWC+QC+HSFWL+GED VCP+C GGFVEELND HDETVQNDFN G E+DLST
Sbjct: 1   MSSFGEDTHWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLST 60

Query: 61  QVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDRF 120
           QVPPIFEAMFALMGRRSPYPRFGLLEAVD FTRERMAGRN NFDVRRRS S+PGQN+D F
Sbjct: 61  QVPPIFEAMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120

Query: 121 NTFWSFHDHVTVPPYSSVTPEDRSSPRSALEILAAQLSLNEHRDPVPAPASHSSIEAMPT 180
           N+FWSFHDH++   +++VTPE RSS  + LE LAAQLSLNE R+PVP PASHS IEAMPT
Sbjct: 121 NSFWSFHDHMSGSTFANVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 180

Query: 181 IKINQMHLGTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240
           IKINQMHLGTDSHCPVCKEKFEL SEAK +PCNHIYH DCILPWLVQHNTCPVCRLELPQ
Sbjct: 181 IKINQMHLGTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPWLVQHNTCPVCRLELPQ 240

Query: 241 QDSGHDWGGT 251
           Q+SGH WGG+
Sbjct: 241 QESGHSWGGS 250

BLAST of Lag0034734 vs. NCBI nr
Match: XP_023531171.1 (probable E3 ubiquitin-protein ligase RHC1A [Cucurbita pepo subsp. pepo] >XP_023531172.1 probable E3 ubiquitin-protein ligase RHC1A [Cucurbita pepo subsp. pepo] >XP_023531173.1 probable E3 ubiquitin-protein ligase RHC1A [Cucurbita pepo subsp. pepo] >XP_023531174.1 probable E3 ubiquitin-protein ligase RHC1A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 448.4 bits (1152), Expect = 4.3e-122
Identity = 209/252 (82.94%), Postives = 223/252 (88.49%), Query Frame = 0

Query: 1   MSSGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLST 60
           MSSGGEDTHWCYQCN S WLNGED  CPYCNGGF+EELNDVHD+TVQ+DF+S  EDDLS+
Sbjct: 1   MSSGGEDTHWCYQCNRSSWLNGEDIACPYCNGGFIEELNDVHDDTVQDDFHSAIEDDLSS 60

Query: 61  QVPPIFEAMFALMGRRSPYPRFGLL-EAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDR 120
           QVPPIFEAMFALMGRR+PYPRF LL EAVDA TRERMAGRN NFDVRRRS+SIPGQN+D 
Sbjct: 61  QVPPIFEAMFALMGRRNPYPRFRLLDEAVDALTRERMAGRNPNFDVRRRSNSIPGQNLDI 120

Query: 121 FNTFWSFHDHVTVPPYSSVTPEDRSSPRSALEILAAQLSLNEHRDPVPAPASHSSIEAMP 180
           FN+FWSFH+H   P ++SV PE RSS   ALE LAA LS NE RDPVP PASHSSIEAMP
Sbjct: 121 FNSFWSFHEHAPGPSFASVAPEGRSSQHVALEALAA-LSFNEQRDPVPTPASHSSIEAMP 180

Query: 181 TIKINQMHLGTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPWLVQHNTCPVCRLELP 240
           TIKINQMHLGTD+HCPVCKEKFELGSEAKEMPCNHIYH DCILPWLVQHNTCPVCRLELP
Sbjct: 181 TIKINQMHLGTDTHCPVCKEKFELGSEAKEMPCNHIYHADCILPWLVQHNTCPVCRLELP 240

Query: 241 QQDSGHDWGGTA 252
           QQDS H WGG +
Sbjct: 241 QQDSRHGWGGNS 251

BLAST of Lag0034734 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 9.2e-43
Identity = 105/259 (40.54%), Postives = 134/259 (51.74%), Query Frame = 0

Query: 7   DTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLSTQVPPIF 66
           +THWC++C  + WL   DAVC YC GGFVEE+ D+       D     E D         
Sbjct: 6   NTHWCHRCQRAVWLRARDAVCSYCGGGFVEEI-DIGPSRAHRD----VERD--------- 65

Query: 67  EAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDRFNTFWSF 126
                        P F L+EA  AF R R+A R+ + ++  R  S   ++         F
Sbjct: 66  -------------PTFDLMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIF 125

Query: 127 ----------HDHVTVPPYSSVTPEDRSSPRS----------ALEILAAQLSL-NEHRDP 186
                      D+ +V  + +         R            LE L  QLS    HR P
Sbjct: 126 GGQAPFRLAGGDNSSVEAFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGP 185

Query: 187 VPAPASHSSIEAMPTIKINQMHL-GTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPW 244
            PAP   SSI+A+PTIKI Q HL  +DSHCPVCK++FEL SEAK+MPC+HIYH+DCI+PW
Sbjct: 186 PPAP--KSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPW 235

BLAST of Lag0034734 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 1.1e-40
Identity = 102/255 (40.00%), Postives = 134/255 (52.55%), Query Frame = 0

Query: 3   SGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLSTQV 62
           S   +THWC++C  +  L+G++ VC YC GGFVEEL                  D++   
Sbjct: 2   SSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEEL------------------DMAQAS 61

Query: 63  PPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDRFNT 122
           P  F+   +  G       F L++A   F R R+A R+ + ++R R+ S   +N      
Sbjct: 62  P--FDMFRSHRGVVERDQTFDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAP 121

Query: 123 FWSFHDHVTVPPYSSVTPE---DRSSP--------------RSALEILAAQLSLNEHRDP 182
              F   V          E   +  SP                 LE L  QLS    R  
Sbjct: 122 LLIFGGQVPYRLTGDNAVEALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRG 181

Query: 183 VPAPASHSSIEAMPTIKINQMHL-GTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPW 240
            P PA  S+I+A+PTIKI Q HL  +DS+CPVCK++FELGSEAK+MPCNHIYH+DCI+PW
Sbjct: 182 -PPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPW 235

BLAST of Lag0034734 vs. ExPASy Swiss-Prot
Match: O22283 (Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RHC2A PE=2 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 3.3e-32
Identity = 101/277 (36.46%), Postives = 140/277 (50.54%), Query Frame = 0

Query: 1   MSSGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDF--NSGTEDDL 60
           M+SG   ++WCY C+   W++ +   CP C+GGF+E + +  D T  + F   + T+   
Sbjct: 1   MASG---SYWCYSCSRFVWVS-DSISCPDCDGGFLELIQEPLDFTPSDSFTTTTTTQHRS 60

Query: 61  STQVPPIFEA----MFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPG 120
            T+ PP   +      ++    SP P           TR R     +   V R S++ P 
Sbjct: 61  PTRFPPPSSSSSTPSASMHADNSPTPTI--------VTRTRSNRSPNPVIVLRGSAAAPS 120

Query: 121 QNV-----DRFNTFWSFHDHVT------VPPYSSVTPEDRSSPRSALEILAAQLSLNEHR 180
            +V     DR + F  ++D  T      +PP  S+T     S    L    +Q+ LN +R
Sbjct: 121 SDVVSEGLDR-SAFQMYYDDGTDSGLRPLPP--SMTEFLLGSGFDRLLDQISQIELNTNR 180

Query: 181 DPVPA---PASHSSIEAMPTIKINQMHLGTD--SHCPVCKEKFELGSEAKEMPCNHIYHT 240
           +       PAS S+IEA+P I+I+  HL +D  SHC VCKE F L S A+EMPCNHIYH 
Sbjct: 181 NLRSCEHPPASKSAIEALPLIEIDPTHLLSDSQSHCAVCKENFVLKSSAREMPCNHIYHP 240

Query: 241 DCILPWLVQHNTCPVCRLELPQQDSGHDWGGTAAATT 256
           DCILPWL   N+CPVCR ELP +D     G    A T
Sbjct: 241 DCILPWLAIRNSCPVCRHELPAEDLTDGTGAALTAVT 262

BLAST of Lag0034734 vs. ExPASy Swiss-Prot
Match: Q7T0Q3 (E3 ubiquitin-protein ligase RNF126-A OS=Xenopus laevis OX=8355 GN=rnf126-a PE=2 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.1e-27
Identity = 81/283 (28.62%), Postives = 133/283 (47.00%), Query Frame = 0

Query: 9   HWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLSTQVPPIFEA 68
           ++C+ C         +  CP C+ GF+EEL     ET +N  N+ + +  + Q  P FE 
Sbjct: 11  YFCHSCTAEITPRLPEYTCPRCDSGFIEEL----PETSRNSENNSSNNSRTDQNRPSFEN 70

Query: 69  M---------------FALMGRRSPYPRFGLLEAVDAF----------TRERMAGR--NS 128
           +               F +      +P FG    V+            +R+R   R   +
Sbjct: 71  LESAQFTLPSGYGQVTFGIFNEGLDFPIFGTSGPVEETRDGESRREHQSRQRYGARQPRA 130

Query: 129 NFDVRR---RSSSIPG-----QNVDRFNTFWSFHDHVTVPPYSSV--TPEDRSSPRSALE 188
               RR   R+  +P      Q +       +   ++ V P+  +   P D +   + L+
Sbjct: 131 RLSTRRAAGRNEGVPTLEGIIQQLVNGIIAPTAMSNLGVGPWGVLHSNPMDYAWGANGLD 190

Query: 189 ILAAQLSLNEHRDPVPAPASHSSIEAMPTIKINQMHLGTDSHCPVCKEKFELGSEAKEMP 248
            +  QL LN+  +  P PA +  I+A+PTI+I + H+G+   CPVCKE + +G   +++P
Sbjct: 191 TIITQL-LNQFENTGPPPADNEKIQALPTIQITEEHVGSGLECPVCKEDYTVGECVRQLP 250

Query: 249 CNHIYHTDCILPWLVQHNTCPVCRLELPQQDSGHDWGGTAAAT 255
           CNH++H DCI+PWL QH+TCPVCR  L  Q++  +  G +  T
Sbjct: 251 CNHLFHNDCIIPWLEQHDTCPVCRKSLSGQNTATNPPGLSEMT 288

BLAST of Lag0034734 vs. ExPASy Swiss-Prot
Match: Q6DIP3 (E3 ubiquitin-protein ligase RNF126 OS=Xenopus tropicalis OX=8364 GN=rnf126 PE=2 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 2.4e-27
Identity = 81/283 (28.62%), Postives = 130/283 (45.94%), Query Frame = 0

Query: 9   HWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLSTQVPPIFEA 68
           ++C+ C         +  CP C+ GF+EEL +  +    +  NSGT+     Q  P FE 
Sbjct: 11  YFCHSCTAEIIPRLPEYTCPRCDSGFIEELPETRNSENNSSNNSGTD-----QNRPSFEN 70

Query: 69  M---------------FALMGRRSPYPRFGLLEAVDA----------FTRERMAGR--NS 128
           +               F +      +P FG    V+            +R+R   R   +
Sbjct: 71  LESAQFTLPSGYGQVTFGIFNEGLDFPIFGTSGPVEEPRDGESRREHQSRQRYGARQPRA 130

Query: 129 NFDVRR---RSSSIPG-----QNVDRFNTFWSFHDHVTVPPYSSV--TPEDRSSPRSALE 188
               RR   R+  +P      Q +       +   ++ V P+  +   P D +   + L+
Sbjct: 131 RLSTRRAAGRNEGVPTLEGIIQQLVNGIIAPTAMSNLGVGPWGVLHSNPMDYAWGANGLD 190

Query: 189 ILAAQLSLNEHRDPVPAPASHSSIEAMPTIKINQMHLGTDSHCPVCKEKFELGSEAKEMP 248
            +  QL LN+  +  P PA    I+A+PTI+I + H+G+   CPVCKE + +G   +++P
Sbjct: 191 TIITQL-LNQFENTGPPPADTEKIQALPTIQITEEHVGSGLECPVCKEDYTVGESVRQLP 250

Query: 249 CNHIYHTDCILPWLVQHNTCPVCRLELPQQDSGHDWGGTAAAT 255
           CNH++H DCI+PWL QH+TCPVCR  L  Q++  +  G    T
Sbjct: 251 CNHLFHNDCIIPWLEQHDTCPVCRKSLSGQNTATNPPGLTEMT 287

BLAST of Lag0034734 vs. ExPASy TrEMBL
Match: A0A6J1D235 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111016591 PE=4 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 4.1e-126
Identity = 209/256 (81.64%), Postives = 232/256 (90.62%), Query Frame = 0

Query: 1   MSSGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGT-EDDLS 60
           MSSGGEDTHWCYQCNHSFWL GED VCPYCNGGF+EELND+HD T+QND N GT E+D S
Sbjct: 1   MSSGGEDTHWCYQCNHSFWLGGEDIVCPYCNGGFIEELNDLHDGTIQNDTNLGTEEEDFS 60

Query: 61  TQVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDR 120
           +QVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRN NFDVRRRSSS+PGQN+D 
Sbjct: 61  SQVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNPNFDVRRRSSSVPGQNLDF 120

Query: 121 FNTFWSFHDHVTVPPYSSVTPEDRSSPRSALEILAAQLSLNEHRDPVPAPASHSSIEAMP 180
           FN+FWSFH+HV  P ++SV PE RSS  S L+ L AQ+++NE RDPVPAPASHSSIEAMP
Sbjct: 121 FNSFWSFHEHVPGPSFASVAPEGRSSQLSGLQALGAQITMNERRDPVPAPASHSSIEAMP 180

Query: 181 TIKINQMHLGTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPWLVQHNTCPVCRLELP 240
           TIKINQMHLGTDSHCPVCKEKFELGSEA+EMPCNHIYH+DCILPWLVQHNTCPVCRLELP
Sbjct: 181 TIKINQMHLGTDSHCPVCKEKFELGSEAREMPCNHIYHSDCILPWLVQHNTCPVCRLELP 240

Query: 241 QQDSGHDWGGTAAATT 256
           QQD+ H W G +++++
Sbjct: 241 QQDTRHAWSGGSSSSS 256

BLAST of Lag0034734 vs. ExPASy TrEMBL
Match: A0A6J1D364 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111016591 PE=4 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 4.1e-126
Identity = 209/256 (81.64%), Postives = 232/256 (90.62%), Query Frame = 0

Query: 1   MSSGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGT-EDDLS 60
           MSSGGEDTHWCYQCNHSFWL GED VCPYCNGGF+EELND+HD T+QND N GT E+D S
Sbjct: 57  MSSGGEDTHWCYQCNHSFWLGGEDIVCPYCNGGFIEELNDLHDGTIQNDTNLGTEEEDFS 116

Query: 61  TQVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDR 120
           +QVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRN NFDVRRRSSS+PGQN+D 
Sbjct: 117 SQVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNPNFDVRRRSSSVPGQNLDF 176

Query: 121 FNTFWSFHDHVTVPPYSSVTPEDRSSPRSALEILAAQLSLNEHRDPVPAPASHSSIEAMP 180
           FN+FWSFH+HV  P ++SV PE RSS  S L+ L AQ+++NE RDPVPAPASHSSIEAMP
Sbjct: 177 FNSFWSFHEHVPGPSFASVAPEGRSSQLSGLQALGAQITMNERRDPVPAPASHSSIEAMP 236

Query: 181 TIKINQMHLGTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPWLVQHNTCPVCRLELP 240
           TIKINQMHLGTDSHCPVCKEKFELGSEA+EMPCNHIYH+DCILPWLVQHNTCPVCRLELP
Sbjct: 237 TIKINQMHLGTDSHCPVCKEKFELGSEAREMPCNHIYHSDCILPWLVQHNTCPVCRLELP 296

Query: 241 QQDSGHDWGGTAAATT 256
           QQD+ H W G +++++
Sbjct: 297 QQDTRHAWSGGSSSSS 312

BLAST of Lag0034734 vs. ExPASy TrEMBL
Match: A0A1S3BQN3 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103492446 PE=4 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 6.9e-126
Identity = 208/255 (81.57%), Postives = 227/255 (89.02%), Query Frame = 0

Query: 1   MSSGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLST 60
           MSS GEDTHWCYQCNHSFWL+GED VCP+CNGGFVEELND HDET+QNDFN G E+DLST
Sbjct: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLST 60

Query: 61  QVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDRF 120
           QVPPIFEAMFALMGRR+PYPRFGLLEAVD FTRERMAGRN NFDVRRRS S+PGQN+D F
Sbjct: 61  QVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120

Query: 121 NTFWSFHDHVTVPPYSSVTPEDRSSPRSALEILAAQLSLNEHRDPVPAPASHSSIEAMPT 180
           N+FWSFHDH++   + +VTPE RSS  + LE LAAQLSLNE R+PVP PASHS IEAMPT
Sbjct: 121 NSFWSFHDHMSGSTFGNVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 180

Query: 181 IKINQMHLGTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240
           IKINQMHLG+DSHCPVCKEKFEL SEAK +PCNHIYHTDCILPWLVQHNTCPVCRLELPQ
Sbjct: 181 IKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240

Query: 241 QDSGHDWGGTAAATT 256
           Q+SGH WGG   + +
Sbjct: 241 QESGHSWGGNGGSNS 255

BLAST of Lag0034734 vs. ExPASy TrEMBL
Match: A0A5A7UIL6 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G00680 PE=4 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 6.9e-126
Identity = 208/255 (81.57%), Postives = 227/255 (89.02%), Query Frame = 0

Query: 1   MSSGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLST 60
           MSS GEDTHWCYQCNHSFWL+GED VCP+CNGGFVEELND HDET+QNDFN G E+DLST
Sbjct: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLST 60

Query: 61  QVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDRF 120
           QVPPIFEAMFALMGRR+PYPRFGLLEAVD FTRERMAGRN NFDVRRRS S+PGQN+D F
Sbjct: 61  QVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120

Query: 121 NTFWSFHDHVTVPPYSSVTPEDRSSPRSALEILAAQLSLNEHRDPVPAPASHSSIEAMPT 180
           N+FWSFHDH++   + +VTPE RSS  + LE LAAQLSLNE R+PVP PASHS IEAMPT
Sbjct: 121 NSFWSFHDHMSGSTFGNVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 180

Query: 181 IKINQMHLGTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240
           IKINQMHLG+DSHCPVCKEKFEL SEAK +PCNHIYHTDCILPWLVQHNTCPVCRLELPQ
Sbjct: 181 IKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240

Query: 241 QDSGHDWGGTAAATT 256
           Q+SGH WGG   + +
Sbjct: 241 QESGHSWGGNGGSNS 255

BLAST of Lag0034734 vs. ExPASy TrEMBL
Match: A0A0A0LZS8 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G600760 PE=4 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 2.9e-124
Identity = 207/250 (82.80%), Postives = 225/250 (90.00%), Query Frame = 0

Query: 1   MSSGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLST 60
           MSS GEDTHWC+QC+HSFWL+GED VCP+C GGFVEELND HDETVQNDFN G E+DLST
Sbjct: 59  MSSFGEDTHWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLST 118

Query: 61  QVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDRF 120
           QVPPIFEAMFALMGRRSPYPRFGLLEAVD FTRERMAGRN NFDVRRRS S+PGQN+D F
Sbjct: 119 QVPPIFEAMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 178

Query: 121 NTFWSFHDHVTVPPYSSVTPEDRSSPRSALEILAAQLSLNEHRDPVPAPASHSSIEAMPT 180
           N+FWSFHDH++   +++VTPE RSS  + LE LAAQLSLNE R+PVP PASHS IEAMPT
Sbjct: 179 NSFWSFHDHMSGSTFANVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 238

Query: 181 IKINQMHLGTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240
           IKINQMHLGTDSHCPVCKEKFEL SEAK +PCNHIYH DCILPWLVQHNTCPVCRLELPQ
Sbjct: 239 IKINQMHLGTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPWLVQHNTCPVCRLELPQ 298

Query: 241 QDSGHDWGGT 251
           Q+SGH WGG+
Sbjct: 299 QESGHSWGGS 308

BLAST of Lag0034734 vs. TAIR 10
Match: AT3G56580.2 (RING/U-box superfamily protein )

HSP 1 Score: 175.3 bits (443), Expect = 6.6e-44
Identity = 105/259 (40.54%), Postives = 134/259 (51.74%), Query Frame = 0

Query: 7   DTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLSTQVPPIF 66
           +THWC++C  + WL   DAVC YC GGFVEE+ D+       D     E D         
Sbjct: 6   NTHWCHRCQRAVWLRARDAVCSYCGGGFVEEI-DIGPSRAHRD----VERD--------- 65

Query: 67  EAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDRFNTFWSF 126
                        P F L+EA  AF R R+A R+ + ++  R  S   ++         F
Sbjct: 66  -------------PTFDLMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIF 125

Query: 127 ----------HDHVTVPPYSSVTPEDRSSPRS----------ALEILAAQLSL-NEHRDP 186
                      D+ +V  + +         R            LE L  QLS    HR P
Sbjct: 126 GGQAPFRLAGGDNSSVEAFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGP 185

Query: 187 VPAPASHSSIEAMPTIKINQMHL-GTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPW 244
            PAP   SSI+A+PTIKI Q HL  +DSHCPVCK++FEL SEAK+MPC+HIYH+DCI+PW
Sbjct: 186 PPAP--KSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPW 235

BLAST of Lag0034734 vs. TAIR 10
Match: AT3G56580.1 (RING/U-box superfamily protein )

HSP 1 Score: 175.3 bits (443), Expect = 6.6e-44
Identity = 105/259 (40.54%), Postives = 134/259 (51.74%), Query Frame = 0

Query: 7   DTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLSTQVPPIF 66
           +THWC++C  + WL   DAVC YC GGFVEE+ D+       D     E D         
Sbjct: 6   NTHWCHRCQRAVWLRARDAVCSYCGGGFVEEI-DIGPSRAHRD----VERD--------- 65

Query: 67  EAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDRFNTFWSF 126
                        P F L+EA  AF R R+A R+ + ++  R  S   ++         F
Sbjct: 66  -------------PTFDLMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIF 125

Query: 127 ----------HDHVTVPPYSSVTPEDRSSPRS----------ALEILAAQLSL-NEHRDP 186
                      D+ +V  + +         R            LE L  QLS    HR P
Sbjct: 126 GGQAPFRLAGGDNSSVEAFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGP 185

Query: 187 VPAPASHSSIEAMPTIKINQMHL-GTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPW 244
            PAP   SSI+A+PTIKI Q HL  +DSHCPVCK++FEL SEAK+MPC+HIYH+DCI+PW
Sbjct: 186 PPAP--KSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPW 235

BLAST of Lag0034734 vs. TAIR 10
Match: AT3G56580.3 (RING/U-box superfamily protein )

HSP 1 Score: 175.3 bits (443), Expect = 6.6e-44
Identity = 105/259 (40.54%), Postives = 134/259 (51.74%), Query Frame = 0

Query: 7   DTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLSTQVPPIF 66
           +THWC++C  + WL   DAVC YC GGFVEE+ D+       D     E D         
Sbjct: 6   NTHWCHRCQRAVWLRARDAVCSYCGGGFVEEI-DIGPSRAHRD----VERD--------- 65

Query: 67  EAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDRFNTFWSF 126
                        P F L+EA  AF R R+A R+ + ++  R  S   ++         F
Sbjct: 66  -------------PTFDLMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIF 125

Query: 127 ----------HDHVTVPPYSSVTPEDRSSPRS----------ALEILAAQLSL-NEHRDP 186
                      D+ +V  + +         R            LE L  QLS    HR P
Sbjct: 126 GGQAPFRLAGGDNSSVEAFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGP 185

Query: 187 VPAPASHSSIEAMPTIKINQMHL-GTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPW 244
            PAP   SSI+A+PTIKI Q HL  +DSHCPVCK++FEL SEAK+MPC+HIYH+DCI+PW
Sbjct: 186 PPAP--KSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPW 235

BLAST of Lag0034734 vs. TAIR 10
Match: AT2G40830.2 (RING-H2 finger C1A )

HSP 1 Score: 168.3 bits (425), Expect = 8.0e-42
Identity = 102/255 (40.00%), Postives = 134/255 (52.55%), Query Frame = 0

Query: 3   SGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLSTQV 62
           S   +THWC++C  +  L+G++ VC YC GGFVEEL                  D++   
Sbjct: 2   SSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEEL------------------DMAQAS 61

Query: 63  PPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDRFNT 122
           P  F+   +  G       F L++A   F R R+A R+ + ++R R+ S   +N      
Sbjct: 62  P--FDMFRSHRGVVERDQTFDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAP 121

Query: 123 FWSFHDHVTVPPYSSVTPE---DRSSP--------------RSALEILAAQLSLNEHRDP 182
              F   V          E   +  SP                 LE L  QLS    R  
Sbjct: 122 LLIFGGQVPYRLTGDNAVEALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRG 181

Query: 183 VPAPASHSSIEAMPTIKINQMHL-GTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPW 240
            P PA  S+I+A+PTIKI Q HL  +DS+CPVCK++FELGSEAK+MPCNHIYH+DCI+PW
Sbjct: 182 -PPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPW 235

BLAST of Lag0034734 vs. TAIR 10
Match: AT2G40830.1 (RING-H2 finger C1A )

HSP 1 Score: 168.3 bits (425), Expect = 8.0e-42
Identity = 102/255 (40.00%), Postives = 134/255 (52.55%), Query Frame = 0

Query: 3   SGGEDTHWCYQCNHSFWLNGEDAVCPYCNGGFVEELNDVHDETVQNDFNSGTEDDLSTQV 62
           S   +THWC++C  +  L+G++ VC YC GGFVEEL                  D++   
Sbjct: 2   SSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEEL------------------DMAQAS 61

Query: 63  PPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNSNFDVRRRSSSIPGQNVDRFNT 122
           P  F+   +  G       F L++A   F R R+A R+ + ++R R+ S   +N      
Sbjct: 62  P--FDMFRSHRGVVERDQTFDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAP 121

Query: 123 FWSFHDHVTVPPYSSVTPE---DRSSP--------------RSALEILAAQLSLNEHRDP 182
              F   V          E   +  SP                 LE L  QLS    R  
Sbjct: 122 LLIFGGQVPYRLTGDNAVEALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRG 181

Query: 183 VPAPASHSSIEAMPTIKINQMHL-GTDSHCPVCKEKFELGSEAKEMPCNHIYHTDCILPW 240
            P PA  S+I+A+PTIKI Q HL  +DS+CPVCK++FELGSEAK+MPCNHIYH+DCI+PW
Sbjct: 182 -PPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPW 235

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022147727.18.4e-12681.64probable E3 ubiquitin-protein ligase RHC1A isoform X1 [Momordica charantia][more]
XP_022147728.18.4e-12681.64probable E3 ubiquitin-protein ligase RHC1A isoform X2 [Momordica charantia][more]
XP_008451039.11.4e-12581.57PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008451040.1... [more]
XP_004144098.16.0e-12482.80E3 ubiquitin-protein ligase RZF1 [Cucumis sativus] >XP_011660068.1 E3 ubiquitin-... [more]
XP_023531171.14.3e-12282.94probable E3 ubiquitin-protein ligase RHC1A [Cucurbita pepo subsp. pepo] >XP_0235... [more]
Match NameE-valueIdentityDescription
Q94AK49.2e-4340.54E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV... [more]
O221971.1e-4040.00Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
O222833.3e-3236.46Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q7T0Q31.1e-2728.62E3 ubiquitin-protein ligase RNF126-A OS=Xenopus laevis OX=8355 GN=rnf126-a PE=2 ... [more]
Q6DIP32.4e-2728.62E3 ubiquitin-protein ligase RNF126 OS=Xenopus tropicalis OX=8364 GN=rnf126 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1D2354.1e-12681.64RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101659... [more]
A0A6J1D3644.1e-12681.64RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101659... [more]
A0A1S3BQN36.9e-12681.57RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103492446 PE=4 ... [more]
A0A5A7UIL66.9e-12681.57RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A0A0LZS82.9e-12482.80RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G600760 PE... [more]
Match NameE-valueIdentityDescription
AT3G56580.26.6e-4440.54RING/U-box superfamily protein [more]
AT3G56580.16.6e-4440.54RING/U-box superfamily protein [more]
AT3G56580.36.6e-4440.54RING/U-box superfamily protein [more]
AT2G40830.28.0e-4240.00RING-H2 finger C1A [more]
AT2G40830.18.0e-4240.00RING-H2 finger C1A [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 194..234
e-value: 3.9E-7
score: 39.7
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 193..235
e-value: 2.2E-11
score: 43.9
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 194..235
score: 12.25323
IPR039525E3 ubiquitin-protein ligase RNF126-like, zinc-ribbonPFAMPF14369zinc_ribbon_9coord: 8..38
e-value: 7.0E-11
score: 42.2
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 158..241
e-value: 1.2E-20
score: 75.0
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 3..247
NoneNo IPR availablePANTHERPTHR15710:SF86E3 UBIQUITIN-PROTEIN LIGASE RHC1A-RELATEDcoord: 3..247
NoneNo IPR availableCDDcd16667RING-H2_RNF126_likecoord: 194..235
e-value: 3.99732E-23
score: 86.6028
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 174..244

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0034734.1Lag0034734.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0061630 ubiquitin protein ligase activity