Lag0034692 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0034692
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNase H domain-containing protein
Locationchr3: 9828816 .. 9829433 (-)
RNA-Seq ExpressionLag0034692
SyntenyLag0034692
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAGCTGAATCTATTTGGAATATCAAAGATCGATGGCTTTGTATTTATAATAATGTTTCTTCACAGACCCCAGAGCGGATATGTATGGTTGCATGGGCTATTTGGAACAACCGTAATAATATAATTCATCAAAGGCCAGTCCCTCCTGTGGGGGTTTTCTGTGAATGGATTCAGGACTATCTCTTTGAATACCACAGATTAGGTTATAAGCGTGGTCGTATGGTTCAATCTTTGGAGAATGTATCGAACATGATCGCTGGAGAGGACGAGATGATTTTGCATGTTGACGCGACATTTGATGAAGAGTCTCGACGTTGTGGAGTGAGGGCAGTTATCAGAACAAGGGAAGGTAAATTTATGTTTATTTTGCATAAAGGTTTCCTTTTGTTCTCTTCCCCTTTATGCGCCGAGGCGGTGGCGGATTTGGAGGGTGTTAAATTGTCAGCTCAACAGAATTTTAAGAAAGTGTCGGTCTTCTCAGATTCTCTATCACTAATCCCTATTCTTCTTCATGGTAAGCAGTGTCAAGCAGATTGTCTCAACACGGTTACGGATATCCAATATTTAAGGAACACTTTCCAGAAGATGAACTTTTGTCATGTCAATCGTGAGTAG

mRNA sequence

ATGGGAGCTGAATCTATTTGGAATATCAAAGATCGATGGCTTTGTATTTATAATAATGTTTCTTCACAGACCCCAGAGCGGATATGTATGGTTGCATGGGCTATTTGGAACAACCGTAATAATATAATTCATCAAAGGCCAGTCCCTCCTGTGGGGGTTTTCTGTGAATGGATTCAGGACTATCTCTTTGAATACCACAGATTAGGTTATAAGCGTGGTCGTATGGTTCAATCTTTGGAGAATGTATCGAACATGATCGCTGGAGAGGACGAGATGATTTTGCATGTTGACGCGACATTTGATGAAGAGTCTCGACGTTGTGGAGTGAGGGCAGTTATCAGAACAAGGGAAGGTAAATTTATGTTTATTTTGCATAAAGGTTTCCTTTTGTTCTCTTCCCCTTTATGCGCCGAGGCGGTGGCGGATTTGGAGGGTGTTAAATTGTCAGCTCAACAGAATTTTAAGAAAGTGTCGGTCTTCTCAGATTCTCTATCACTAATCCCTATTCTTCTTCATGGTAAGCAGTGTCAAGCAGATTGTCTCAACACGGTTACGGATATCCAATATTTAAGGAACACTTTCCAGAAGATGAACTTTTGTCATGTCAATCGTGAGTAG

Coding sequence (CDS)

ATGGGAGCTGAATCTATTTGGAATATCAAAGATCGATGGCTTTGTATTTATAATAATGTTTCTTCACAGACCCCAGAGCGGATATGTATGGTTGCATGGGCTATTTGGAACAACCGTAATAATATAATTCATCAAAGGCCAGTCCCTCCTGTGGGGGTTTTCTGTGAATGGATTCAGGACTATCTCTTTGAATACCACAGATTAGGTTATAAGCGTGGTCGTATGGTTCAATCTTTGGAGAATGTATCGAACATGATCGCTGGAGAGGACGAGATGATTTTGCATGTTGACGCGACATTTGATGAAGAGTCTCGACGTTGTGGAGTGAGGGCAGTTATCAGAACAAGGGAAGGTAAATTTATGTTTATTTTGCATAAAGGTTTCCTTTTGTTCTCTTCCCCTTTATGCGCCGAGGCGGTGGCGGATTTGGAGGGTGTTAAATTGTCAGCTCAACAGAATTTTAAGAAAGTGTCGGTCTTCTCAGATTCTCTATCACTAATCCCTATTCTTCTTCATGGTAAGCAGTGTCAAGCAGATTGTCTCAACACGGTTACGGATATCCAATATTTAAGGAACACTTTCCAGAAGATGAACTTTTGTCATGTCAATCGTGAGTAG

Protein sequence

MGAESIWNIKDRWLCIYNNVSSQTPERICMVAWAIWNNRNNIIHQRPVPPVGVFCEWIQDYLFEYHRLGYKRGRMVQSLENVSNMIAGEDEMILHVDATFDEESRRCGVRAVIRTREGKFMFILHKGFLLFSSPLCAEAVADLEGVKLSAQQNFKKVSVFSDSLSLIPILLHGKQCQADCLNTVTDIQYLRNTFQKMNFCHVNRE
Homology
BLAST of Lag0034692 vs. NCBI nr
Match: XP_012487919.1 (PREDICTED: uncharacterized protein LOC105801131 [Gossypium raimondii])

HSP 1 Score: 85.1 bits (209), Expect = 7.6e-13
Identity = 52/194 (26.80%), Postives = 94/194 (48.45%), Query Frame = 0

Query: 13  WLCIYNNVSSQTPERI-CMVAWAIWNNRNNIIHQRPVPPVGVFCEWIQDYLFEYHRLGYK 72
           WL    + +S++  RI C   WAIW +RNN +H +          ++  Y+ E   +G  
Sbjct: 45  WLTGVFSKNSESQCRIFCYALWAIWGDRNNKVHNKVSKSGKEIGRFVSSYITELDGIGKS 104

Query: 73  RGRMVQSLENVSNMIAGEDEMI-LHVDATFDEESRRCGVRAVIRTREGKFMFILHKGFLL 132
           +    QS +  S      D+++ ++ DA +D +S++     V R  EGK +      +  
Sbjct: 105 K---AQSYKLTSKWKKPHDQIVKINFDAAYDGKSKQSAAGIVTRNNEGKVLLSCLMIYQQ 164

Query: 133 FSSPLCAEAVADLEGVKLSAQQNFKKVSVFSDSLSLIPILLHGKQCQADCLNTVTDIQYL 192
            +SP  AEA+A  + +K+  +  ++K+ +  DSLS+I         ++     ++DI  L
Sbjct: 165 VASPFAAEALASRKAIKIGKEMKWRKIIIEGDSLSIIKKCRSNIPDKSQVCAYISDIHKL 224

Query: 193 RNTFQKMNFCHVNR 205
           ++ FQ+  F HV R
Sbjct: 225 QSRFQECRFEHVPR 235

BLAST of Lag0034692 vs. NCBI nr
Match: MBA0701905.1 (hypothetical protein [Gossypium aridum])

HSP 1 Score: 84.7 bits (208), Expect = 9.9e-13
Identity = 49/194 (25.26%), Postives = 91/194 (46.91%), Query Frame = 0

Query: 13  WLC-IYNNVSSQTPERICMVAWAIWNNRNNIIHQRPVPPVGVFCEWIQDYLFEYHRLGYK 72
           WL  ++   S    +  C   WAIW +RNN +H +          ++  Y+ E   +G  
Sbjct: 184 WLTEVFVKNSESQCKMFCCSLWAIWGDRNNRVHNKVSKSGKEISRFVSSYIQELDGIGKS 243

Query: 73  RGRMVQSLENVSNMIAGEDEMI-LHVDATFDEESRRCGVRAVIRTREGKFMFILHKGFLL 132
           +   V+S ++ S      D+++ ++ DA +DE+S+      V R  E K +      +  
Sbjct: 244 K---VESYKSTSKWKKPPDQIVKINFDAAYDEKSKESAAGIVARNNESKVLLSCSMIYKH 303

Query: 133 FSSPLCAEAVADLEGVKLSAQQNFKKVSVFSDSLSLIPILLHGKQCQADCLNTVTDIQYL 192
            +SP  AE +A  + +K   +  ++K+ +  DSLS+I         ++     ++DI  L
Sbjct: 304 VASPFAAETLASRKAIKTGKEMKWRKIIIEGDSLSIIKKCRSNIPVKSKVCTYISDIHKL 363

Query: 193 RNTFQKMNFCHVNR 205
           +++FQ+  F HV R
Sbjct: 364 QSSFQECRFEHVPR 374

BLAST of Lag0034692 vs. NCBI nr
Match: XP_030975730.1 (uncharacterized protein LOC115995344 [Quercus lobata])

HSP 1 Score: 78.6 bits (192), Expect = 7.1e-11
Identity = 53/194 (27.32%), Postives = 90/194 (46.39%), Query Frame = 0

Query: 15  CIYNNVSSQTPERICMVAWAIWNNRNNIIHQRPVPPVGVFCEWIQDYLFEYHRLGYKR-- 74
           C++    ++ P    MVAWAIWN RNN+   +P  P+       QD + E+    +    
Sbjct: 58  CVF--ADNRDPTLFSMVAWAIWNRRNNLRLGKPAAPLNELIPQAQDRMREFKLYNFSTII 117

Query: 75  --GRMVQSLENVSNMIAGEDEMILHVDATFDEESRRCGVRAVIRTREGKFMFILHKGFLL 134
             GR   S +  S+ I       ++ D          G+  VIR  EG+ M  L +   L
Sbjct: 118 PVGRPPTSWQAPSSNI-----YKVNFDGAQFVVENSAGLGVVIRNSEGQVMLSLSEKTPL 177

Query: 135 FSSPLCAEAVADLEGVKLSAQQNFKKVSVFSDSLSLIPILLHGKQCQADCLNTVTDIQYL 194
             + +  EA+A    +KL+ +  F+++++  DS  LI  L++     +   + + DIQ+L
Sbjct: 178 PFTAIEVEAMAARRALKLALETGFQQITLEGDSQILISALVNNTHSLSSFGHILKDIQFL 237

Query: 195 RNTFQKMNFCHVNR 205
            + F  +N+ HV R
Sbjct: 238 ASCFSTINYSHVRR 244

BLAST of Lag0034692 vs. NCBI nr
Match: KAG8478291.1 (hypothetical protein CXB51_028103 [Gossypium anomalum])

HSP 1 Score: 78.6 bits (192), Expect = 7.1e-11
Identity = 47/189 (24.87%), Postives = 92/189 (48.68%), Query Frame = 0

Query: 17  YNNVSSQTPERICMVAWAIWNNRNNIIHQRPVPPVGVFCEWIQDYLFEYHRLGYKRGRMV 76
           +N+++++  +   +  W IW N+N I H+     V     +++ Y  E   +     R V
Sbjct: 3   FNSLNNKERKIRVIAYWVIWYNQNKIYHEGAREQVNEVVSFVKAYCLEITAMEVTM-RSV 62

Query: 77  QSLENVSNMIAGEDEMILHVDATFDEESRRCGVRAVIRTREGKFMFILHKGFLLFSSPLC 136
           Q + N      G D + ++ DATF++ +RR     + R ++G  M      +   S P+ 
Sbjct: 63  QVVGNYDWSPPGNDTIKINFDATFNQHTRRSNSGVIARNKDGLVMAACTFLWAYISGPVM 122

Query: 137 AEAVADLEGVKLSAQQNFKKVSVFSDSLSLIPILLHGKQCQADCLNTVTDIQYLRNTFQK 196
           AEA A L+ V ++ +  F  + +  DSL++I  L   ++ +++  + V +I+     F+ 
Sbjct: 123 AEARACLQVVSMAEEMGFHDICIKGDSLTIIRKLNTAEEDKSEISSLVKEIKGRAYIFRS 182

Query: 197 MNFCHVNRE 206
           + F H+ RE
Sbjct: 183 VRFRHIPRE 190

BLAST of Lag0034692 vs. NCBI nr
Match: XP_030969948.1 (uncharacterized protein LOC115990241 [Quercus lobata])

HSP 1 Score: 77.8 bits (190), Expect = 1.2e-10
Identity = 49/176 (27.84%), Postives = 81/176 (46.02%), Query Frame = 0

Query: 30  MVAWAIWNNRNNIIHQRPVPPVGVFCEWIQDYLFEYHRLGYKRGRMVQSLENVSNMIAGE 89
           MV WA+WN RNN+   +   P+ +  +  + +L ++      R   V      S    G 
Sbjct: 1   MVVWALWNRRNNLRLGKKAEPLNILMQKAKPHLGDFQHYNTARVEPV-GRPPTSWQPPGP 60

Query: 90  DEMILHVDATFDEESRRCGVRAVIRTREGKFMFILHKGFLLFSSPLCAEAVADLEGVKLS 149
            +  +++D    E     G R VIR   G+ M  L +   L SS L  EA+A   G++L 
Sbjct: 61  FQYKVNMDGALFEADNTAGPRVVIRNEHGQVMASLSERIRLPSSVLEVEALAARRGLELV 120

Query: 150 AQQNFKKVSVFSDSLSLIPILLHGKQCQADCLNTVTDIQYLRNTFQKMNFCHVNRE 206
            +  F+ + + SDS  LI  L  G    +   + V DI+++ +    +N+ HV R+
Sbjct: 121 VETGFRNIVLESDSQILITTLREGSYSLSSFGHIVQDIKFIASYVSSINYTHVRRQ 175

BLAST of Lag0034692 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.7e-10
Identity = 55/210 (26.19%), Postives = 90/210 (42.86%), Query Frame = 0

Query: 1    MGAESIWNIKDRWLCIYNNVSSQTPERICMVAWAIWNNRNNIIHQRPVPPVGVFCEWIQD 60
            + AE   +  + W  +   +  +      +  W IWN+RN++IH + V PV   CEW+  
Sbjct: 1207 LSAEDNISFLELWSSLTEQLEPKDLNLAAITGWGIWNDRNSLIHGKQVSPVEFKCEWLTP 1266

Query: 61   YLFEYHRLGYKRGRMVQSLENVSNMI-----AGEDEMILHVDATFDEESRRCGVRAVIRT 120
            +L + H          ++  N   ++     +    + L+ DA     S   G   +IR 
Sbjct: 1267 FL-DSHSQAQMSNYSPRTQSNHRPVVQYWRPSSSVSLKLNTDAACRGASTSFG--CIIRD 1326

Query: 121  REGKFMFILHKGFLLFSSPLCAEAVADLEGVKLSAQQNFKKVSVFSDSLSLIPILLHGKQ 180
                 +           SPL AE    LEG+K +A  NF  + V SDSL  I ++ +   
Sbjct: 1327 SSCSLVAATSIRVPFPLSPLLAEIRCILEGLKFAAASNFTHLEVESDSLLAIQLIRNEIH 1386

Query: 181  CQADCLNTVTDIQYLRNTFQKMNFCHVNRE 206
             + D  N V +IQ L   F  ++F H +R+
Sbjct: 1387 TRGDEQNWVMEIQALTCCFAFISFSHSSRQ 1413

BLAST of Lag0034692 vs. ExPASy TrEMBL
Match: A0A5B6VK73 (Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_015215 PE=4 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 2.2e-10
Identity = 48/190 (25.26%), Postives = 86/190 (45.26%), Query Frame = 0

Query: 16  IYNNVSSQTPERICMVAWAIWNNRNNIIHQRPVPPVGVFCEWIQDYLFEYHRLGYKRGRM 75
           ++   S+Q    I    WA+W +RN +IH+R +        +I DYL E    G  +   
Sbjct: 47  LFERESNQRIRTIVCAIWALWTDRNKLIHERKMQTSANLTHFIIDYLRELD--GVNKRLP 106

Query: 76  VQSLENVSNMIAGEDEMILHVDATFDEESRRCGVRAVIRTREGKFMFILHKGFLLFSSPL 135
           +  L N S     +  + ++ DA FD++ +R     VIR   G+ +   H       S  
Sbjct: 107 ISKLVNESWRPPEDINVKINFDAAFDKQHKRSCTEIVIRNSSGQVLKTKH-----IPSTF 166

Query: 136 CAEAVADLEGVKLSAQQNFKKVSVFSDSLSLIPILLHGKQCQADCLNTVTDIQYLRNTFQ 195
            AEA+  ++ V+  A+  F +V V   +LS+I  +L     +++  + + D + L++ F 
Sbjct: 167 AAEALVCVQAVRFGAESGFLRVEVEGGTLSVIKKILCEDDDRSEIRSYILDAKRLKSNFI 226

Query: 196 KMNFCHVNRE 206
                H  R+
Sbjct: 227 NCRLKHEGRQ 229

BLAST of Lag0034692 vs. ExPASy TrEMBL
Match: A0A2P5EP22 (Ribonuclease H-like domain containing protein OS=Trema orientale OX=63057 GN=TorRG33x02_169220 PE=4 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 5.5e-09
Identity = 48/168 (28.57%), Postives = 79/168 (47.02%), Query Frame = 0

Query: 26  ERICMVAWAIWNNRNNIIHQRPVPPVGVFCEWIQDYLFEYHRLGYKRGRMVQSLENVSNM 85
           E  C++ WAIWN RN+++H +         +W+Q +LFE+   G K    V S+    N+
Sbjct: 22  EVFCVLLWAIWNRRNDVLHNQNSKEAAALLDWVQSFLFEFQD-GLKIVN-VYSICQGRNL 81

Query: 86  I----AGEDEMILHVDATFDEESRRCGVRAVIRTREGKFMFILHKGFLLFSSPLCAEAVA 145
           +         + L+VDA   ++S   GV   IR  EG  +       + +   + +E +A
Sbjct: 82  VKWCKPSSGSLKLNVDAAVIKDSGVIGVGGAIRDLEGLVLACWSLQLVGYFDIVTSELLA 141

Query: 146 DLEGVKLSAQQNFKKVSVFSDSLSLIPILLHGKQCQADCLNTVTDIQY 190
             EGV+L+ +      S+ SDSLS +  +     C A   + V DI +
Sbjct: 142 IREGVRLAVEFGCPLASIESDSLSAVQAISSPSPCFAGA-SIVNDILF 186

BLAST of Lag0034692 vs. ExPASy TrEMBL
Match: A0A1U8JJX9 (uncharacterized protein LOC107907782 OS=Gossypium hirsutum OX=3635 GN=LOC107907782 PE=4 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 9.4e-09
Identity = 51/206 (24.76%), Postives = 98/206 (47.57%), Query Frame = 0

Query: 4   ESIWNIKDRWLCI-YNNVSSQTPERICMVAWAIWNNRNNIIHQRPVPPVGVFCEWIQDYL 63
           ES W    +WL + + N S++  +   +  WA+W N N I H+           +I  Y 
Sbjct: 52  ESNWK---KWLALEFENYSNEACKIRAISFWALWYNCNRISHEGIRERAHEIVRFINAYY 111

Query: 64  FEYHRLGYKRGRMVQSLENVSNMI---AGEDEMILHVDATFDEESRRCGVRAVIRTREGK 123
            E  ++    G ++++ +     +     +D + ++ DA+F++ SRR     + R +EG 
Sbjct: 112 SEITQM----GEILKNRQETKRFVWEPPVDDAVKINFDASFNQHSRRSCSLVIARNKEGL 171

Query: 124 FMFILHKGFLLFSSPLCAEAVADLEGVKLSAQQNFKKVSVFSDSLSLIPILLHGKQCQAD 183
            M      +   S P+ AEA+A L+ V ++ +  F+ + V  D+L++I  L   K+ ++ 
Sbjct: 172 VMASCTYSWENISDPVIAEAMACLQAVTMAEEIGFQDICVEGDALTVIRKLNSTKEDRSC 231

Query: 184 CLNTVTDIQYLRNTFQKMNFCHVNRE 206
             + + +I      F++M F  V RE
Sbjct: 232 ISSMIQEINGRTPNFRRMCFRFVPRE 250

BLAST of Lag0034692 vs. ExPASy TrEMBL
Match: A0A5B6VXJ9 (Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_024353 PE=4 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.2e-08
Identity = 50/198 (25.25%), Postives = 90/198 (45.45%), Query Frame = 0

Query: 11  DRWLC-IYNNVSSQTPERICMVAWAIWNNRNNIIHQRPVPPVGVFCEWIQDYLFEYHRLG 70
           ++WL  ++  V+       C   WAIW +RN+ IH + V        +I +YL E   L 
Sbjct: 185 EQWLIWVFEQVNLHQCRIFCCALWAIWGDRNSRIHNKKVSTEAEIGNFIINYLAEIDGLE 244

Query: 71  YKRGRMVQSLENVSNMIAGEDEMILHVDATFDEESRRCGVRAVIRTREGKFMFI---LHK 130
            ++   +   +  +      D + ++VD  FD  +       V+R  EG  +     +HK
Sbjct: 245 RRKHGTIDIFQKWT--YPPPDSVKINVDGAFDSHNNISTSGVVVRDNEGSVLLSWSKIHK 304

Query: 131 GFLLFSSPLCAEAVADLEGVKLSAQQNFKKVSVFSDSLSLIPILLHGKQCQADCLNTVTD 190
           G     S   AEA+A  E V++  +  + K+ +  DSL++I    +  + ++     + D
Sbjct: 305 G---VHSAFEAEAMACREAVQIGVEHGWPKIIIEGDSLTVIKKCQNQDRDRSMLGAFIQD 364

Query: 191 IQYLRNTFQKMNFCHVNR 205
           IQ + ++ +K  F HV R
Sbjct: 365 IQMMVSSSRKFVFKHVPR 377

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_012487919.17.6e-1326.80PREDICTED: uncharacterized protein LOC105801131 [Gossypium raimondii][more]
MBA0701905.19.9e-1325.26hypothetical protein [Gossypium aridum][more]
XP_030975730.17.1e-1127.32uncharacterized protein LOC115995344 [Quercus lobata][more]
KAG8478291.17.1e-1124.87hypothetical protein CXB51_028103 [Gossypium anomalum][more]
XP_030969948.11.2e-1027.84uncharacterized protein LOC115990241 [Quercus lobata][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DX301.7e-1026.19uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A5B6VK732.2e-1025.26Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_015215 PE=4 SV=1[more]
A0A2P5EP225.5e-0928.57Ribonuclease H-like domain containing protein OS=Trema orientale OX=63057 GN=Tor... [more]
A0A1U8JJX99.4e-0924.76uncharacterized protein LOC107907782 OS=Gossypium hirsutum OX=3635 GN=LOC1079077... [more]
A0A5B6VXJ91.2e-0825.25Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_024353 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 96..205
e-value: 5.3E-14
score: 52.1
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 91..205
e-value: 1.2E-6
score: 30.4
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 94..205
e-value: 2.45624E-12
score: 59.25
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 91..204

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0034692.1Lag0034692.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity