Lag0034579 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0034579 vs. NCBI nr
Match: XP_022933888.1 (protein ALP1-like [Cucurbita moschata] >KAG7021950.1 Protein ALP1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 834.7 bits (2155), Expect = 3.9e-238
Identity = 416/481 (86.49%), Postives = 438/481 (91.06%), Query Frame = 0

Query: 1   MEISSFPFLNQEDLLPIFNLFSEMDNSFSLNQTPKKRRRPEDNGDDH------SQFDGND 60
           MEISSFPFLNQ+DLLPIFNLFSEMD++FS+NQ+PKKRRR  D+ DDH      + FD ++
Sbjct: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNQSPKKRRRQNDDDDDHQTQFNKTSFDDDE 60

Query: 61  LLKLPLWFDTHDDKPDHSWIMDSDADHKREFHLSDESFPQF-SKKPRRETPVSANSSPAK 120
           LLKLP WFD  DDK  H WIM    + K EF +SDE+  QF  KKPRR TP + +SSPAK
Sbjct: 61  LLKLPFWFD--DDKQQH-WIM----EQKPEFQVSDENLTQFVPKKPRRATPENTHSSPAK 120

Query: 121 GGGTGGQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKD 180
            GG G Q RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSK+TFDMIC+ELDSTVMKKD
Sbjct: 121 -GGAGTQHRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKD 180

Query: 181 TMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFL 240
           TMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFL
Sbjct: 181 TMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFL 240

Query: 241 QWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYS 300
           QWP++SKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYS
Sbjct: 241 QWPEESKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYS 300

Query: 301 ITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFIVGNSGYPLTDW 360
           ITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLL DV IVGNSGYPLTDW
Sbjct: 301 ITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDW 360

Query: 361 LLVPYSLQNLTWTQHAFNEKVSEIQAAAKAAFGRLKGRWSCLQKRTEVKLQELPVVLGAC 420
           LLVPYS QNLTWTQHAFNEKVSEIQ AAKAAFGRLKGRW+CLQKRTEVKLQELPVVLGAC
Sbjct: 361 LLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGAC 420

Query: 421 CVLHNICEMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDHIAHNLLHHGLAGTGF 475
           CVLHNICEMRKE FDPEL FE FDDEM+PE+NG+RS SAIQARDHIAHNLLHHGLAGTGF
Sbjct: 421 CVLHNICEMRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGLAGTGF 473

BLAST of Lag0034579 vs. NCBI nr
Match: KAG6588056.1 (Protein ALP1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 833.6 bits (2152), Expect = 8.7e-238
Identity = 415/481 (86.28%), Postives = 438/481 (91.06%), Query Frame = 0

Query: 1   MEISSFPFLNQEDLLPIFNLFSEMDNSFSLNQTPKKRRRPEDNGDDH------SQFDGND 60
           MEISSFPFLNQ+DL+PIFNLFSEMD++FS+NQ+PKKRRR  D+ DDH      + FD ++
Sbjct: 1   MEISSFPFLNQDDLVPIFNLFSEMDDNFSVNQSPKKRRRQNDDDDDHQTQFNKTSFDDDE 60

Query: 61  LLKLPLWFDTHDDKPDHSWIMDSDADHKREFHLSDESFPQF-SKKPRRETPVSANSSPAK 120
           LLKLP WFD  DDK  H WIM    + K EF +SDE+  QF  KKPRR TP + +SSPAK
Sbjct: 61  LLKLPFWFD--DDKQQH-WIM----EQKPEFQVSDENLTQFVPKKPRRATPENTHSSPAK 120

Query: 121 GGGTGGQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKD 180
            GG G Q RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSK+TFDMIC+ELDSTVMKKD
Sbjct: 121 -GGAGTQHRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKD 180

Query: 181 TMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFL 240
           TMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFL
Sbjct: 181 TMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFL 240

Query: 241 QWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYS 300
           QWP++SKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYS
Sbjct: 241 QWPEESKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYS 300

Query: 301 ITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFIVGNSGYPLTDW 360
           ITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLL DV IVGNSGYPLTDW
Sbjct: 301 ITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDW 360

Query: 361 LLVPYSLQNLTWTQHAFNEKVSEIQAAAKAAFGRLKGRWSCLQKRTEVKLQELPVVLGAC 420
           LLVPYS QNLTWTQHAFNEKVSEIQ AAKAAFGRLKGRW+CLQKRTEVKLQELPVVLGAC
Sbjct: 361 LLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGAC 420

Query: 421 CVLHNICEMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDHIAHNLLHHGLAGTGF 475
           CVLHNICEMRKE FDPEL FE FDDEM+PE+NG+RS SAIQARDHIAHNLLHHGLAGTGF
Sbjct: 421 CVLHNICEMRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGLAGTGF 473

BLAST of Lag0034579 vs. NCBI nr
Match: XP_038880089.1 (protein ALP1-like [Benincasa hispida])

HSP 1 Score: 830.9 bits (2145), Expect = 5.7e-237
Identity = 417/490 (85.10%), Postives = 441/490 (90.00%), Query Frame = 0

Query: 1   MEISSFPFLNQEDLLPIFNLFSEMDN-------SFSLNQTPKKRRRPEDNGDDHSQFDG- 60
           MEISSFPFLNQE+LLPIFNLFS+MDN       +FS+NQ+PKKRRR ++NGDDHSQF+  
Sbjct: 1   MEISSFPFLNQEELLPIFNLFSDMDNNHNNTNATFSVNQSPKKRRRSDENGDDHSQFNNI 60

Query: 61  ----ND--LLKLPLWFDTHDDKPDHSWIMDSDADHKR-EFHLSDESFPQFSKKPRRETPV 120
               ND  L KLP WF++ +     +WIMDS+    R EFHLSD++  QFSKKPRR T  
Sbjct: 61  SFTENDEALQKLPCWFESQE-----NWIMDSEEPKPRNEFHLSDQNPTQFSKKPRRTT-- 120

Query: 121 SANSSPAKG-GGTGGQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKE 180
           + N SPAK   G G QQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKE
Sbjct: 121 AENGSPAKNPTGGGAQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKE 180

Query: 181 LDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAI 240
           LDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAI
Sbjct: 181 LDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAI 240

Query: 241 RKVLMPKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHT 300
           RKVLMPKFLQWPD+SKL KIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHT
Sbjct: 241 RKVLMPKFLQWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHT 300

Query: 301 ERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFIVG 360
           ERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKS LFERANMGLLNDVFIVG
Sbjct: 301 ERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSVLFERANMGLLNDVFIVG 360

Query: 361 NSGYPLTDWLLVPYSLQNLTWTQHAFNEKVSEIQAAAKAAFGRLKGRWSCLQKRTEVKLQ 420
           NSGYPL DWLLVPY++QNLTWTQH FNEKV EIQAAAK AFGRLKGRWSCLQKRTEVKLQ
Sbjct: 361 NSGYPLMDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKTAFGRLKGRWSCLQKRTEVKLQ 420

Query: 421 ELPVVLGACCVLHNICEMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDHIAHNLL 475
           ELPVVLGACCVLHNICE+RKE FDP+L FEL+DDEM+PE+NGLRSVSAIQARDHIAHNLL
Sbjct: 421 ELPVVLGACCVLHNICEIRKEKFDPDLKFELYDDEMVPENNGLRSVSAIQARDHIAHNLL 480

BLAST of Lag0034579 vs. NCBI nr
Match: XP_023529395.1 (protein ALP1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 830.5 bits (2144), Expect = 7.4e-237
Identity = 417/482 (86.51%), Postives = 436/482 (90.46%), Query Frame = 0

Query: 1   MEISSFPFLNQEDLLPIFNLFSEMDNSFSLNQTPKKRRRPEDNGDDHSQ-------FDGN 60
           MEISSFPFLNQ+DLLPIFNLFSEMD++FS+N +PKKRRR +DN DD  Q       FD +
Sbjct: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNHSPKKRRR-QDNDDDDDQTQFNKTSFDDD 60

Query: 61  DLLKLPLWFDTHDDKPDHSWIMDSDADHKREFHLSDESFPQF-SKKPRRETPVSANSSPA 120
           +LLKLP WFD  DDK  H WIM    + K EF +SDE+  QF  KKPRR TP + +SSP 
Sbjct: 61  ELLKLPFWFD--DDKQQH-WIM----EQKPEFQVSDENLTQFVPKKPRRATPENTHSSPV 120

Query: 121 KGGGTGGQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKK 180
           K GG G Q RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSK+TFDMICKELDSTVMKK
Sbjct: 121 K-GGAGTQHRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICKELDSTVMKK 180

Query: 181 DTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKF 240
           DTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKF
Sbjct: 181 DTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKF 240

Query: 241 LQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSY 300
           LQWP+DSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSY
Sbjct: 241 LQWPEDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSY 300

Query: 301 SITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFIVGNSGYPLTD 360
           SITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLL DV IVGNSGYPLTD
Sbjct: 301 SITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTD 360

Query: 361 WLLVPYSLQNLTWTQHAFNEKVSEIQAAAKAAFGRLKGRWSCLQKRTEVKLQELPVVLGA 420
           WLLVPYS QNLTWTQHAFNEKVSEIQ AAKAAFGRLKGRW+CLQKRTEVKLQELPVVLGA
Sbjct: 361 WLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGA 420

Query: 421 CCVLHNICEMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDHIAHNLLHHGLAGTG 475
           CCVLHNICEMRKE FDPEL FE FDDEM+PE+NG+RS SAIQARDHIAHNLLHHGLAGTG
Sbjct: 421 CCVLHNICEMRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGLAGTG 473

BLAST of Lag0034579 vs. NCBI nr
Match: XP_022967341.1 (protein ALP1-like [Cucurbita maxima])

HSP 1 Score: 828.6 bits (2139), Expect = 2.8e-236
Identity = 413/480 (86.04%), Postives = 437/480 (91.04%), Query Frame = 0

Query: 1   MEISSFPFLNQEDLLPIFNLFSEMDNSFSLNQTPKKRRRPEDNGDDHSQ-----FDGNDL 60
           MEISSFPFLNQ+DLLPIFNLFSEMD++FS+N +PKKRRR +D+ DD +Q     FD ++L
Sbjct: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNYSPKKRRRQDDDDDDQTQFNKTSFDDDEL 60

Query: 61  LKLPLWFDTHDDKPDHSWIMDSDADHKREFHLSDESFPQF-SKKPRRETPVSANSSPAKG 120
           LKLP WFD  DDK  H WIM    + K EF + DE+  QF +KKPRR TP + +SSPAK 
Sbjct: 61  LKLPFWFD--DDKQQH-WIM----EQKPEFQVFDENLTQFVAKKPRRATPENTHSSPAK- 120

Query: 121 GGTGGQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDT 180
           GG G Q RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSK+TFDMIC+ELDSTVMKKDT
Sbjct: 121 GGAGTQHRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKDT 180

Query: 181 MLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQ 240
           MLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQ
Sbjct: 181 MLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQ 240

Query: 241 WPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSI 300
           WP+DSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSI
Sbjct: 241 WPEDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSI 300

Query: 301 TVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFIVGNSGYPLTDWL 360
           TVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLL DV IVGNSGYPLTDWL
Sbjct: 301 TVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDWL 360

Query: 361 LVPYSLQNLTWTQHAFNEKVSEIQAAAKAAFGRLKGRWSCLQKRTEVKLQELPVVLGACC 420
           LVPYS QNLTWTQHAFNEKVSEIQ AAKAAFGRLKGRW+CLQKRTEVKLQELPVVLGACC
Sbjct: 361 LVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACC 420

Query: 421 VLHNICEMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDHIAHNLLHHGLAGTGFL 475
           VLHNICEMRKE FDPEL FE FDDEM+PE+NG+RS SAI ARDHI+HNLLHHGLAGTGFL
Sbjct: 421 VLHNICEMRKERFDPELKFEFFDDEMVPENNGVRSASAILARDHISHNLLHHGLAGTGFL 472

BLAST of Lag0034579 vs. ExPASy Swiss-Prot
Match: Q9M2U3 (Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 4.3e-38
Identity = 113/362 (31.22%), Postives = 175/362 (48.34%), Query Frame = 0

Query: 129 RSKDWWDQCNHPDF----PDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVA----I 188
           +S DWWD  +   +      + F   F++S+ TFD IC  + +    K      +    +
Sbjct: 50  QSLDWWDGFSRRIYGGSTDPKTFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPL 109

Query: 189 PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDDSK 248
            +  RVAV + RL +GE L ++ + FG+  ST  ++      ++ +  +   L WP  SK
Sbjct: 110 SLNDRVAVALRRLGSGESLSVIGETFGMNQSTVSQITWRFVESMEERAI-HHLSWP--SK 169

Query: 249 LAKIKQEFESISGIPKVGGSIYTTHI----PIIAPKNNVAAYFNKRHTERNQKTSYSITV 308
           L +IK +FE ISG+P   G+I  THI    P + P N V           + + ++S+T+
Sbjct: 170 LDEIKSKFEKISGLPNCCGAIDITHIVMNLPAVEPSNKVWL---------DGEKNFSMTL 229

Query: 309 QGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMG-LLND------------VFIV 368
           Q VVDP   F DV  GWPGS+ DD VL+ S  ++    G  LN              +IV
Sbjct: 230 QAVVDPDMRFLDVIAGWPGSLNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIV 289

Query: 369 GNSGYPLTDWLLVPYSLQNLTWTQHAFNEKVSEIQAAAKAAFGRLKGRWSCLQKRTEV-K 428
           G+SG+PL  WLL PY  +  +  Q  FN++ SE   AA+ A  +LK RW  +     +  
Sbjct: 290 GDSGFPLLPWLLTPYQGKPTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPD 349

Query: 429 LQELPVVLGACCVLHN-ICEMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDHIAH 464
              LP ++  CC+LHN I +M  ++ D +   +  D      S  L   ++   RD ++ 
Sbjct: 350 RNRLPRIIFVCCLLHNIIIDMEDQTLDDQPLSQQHDMNYRQRSCKLADEASSVLRDELSD 399

BLAST of Lag0034579 vs. ExPASy Swiss-Prot
Match: Q94K49 (Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=ALP1 PE=1 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 3.8e-34
Identity = 94/314 (29.94%), Postives = 156/314 (49.68%), Query Frame = 0

Query: 132 DWWD----QCNHPDFPDEE---FRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAI---- 191
           DWWD    + + P  P +E   F+  FR SK+TF  IC  +   ++ +     + I    
Sbjct: 43  DWWDTFWLRNSSPSVPSDEDYAFKHFFRASKTTFSYICSLVREDLISRPPSGLINIEGRL 102

Query: 192 -PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDDS 251
             V ++VA+ + RLA+G+    V   FG+G ST  ++      A+ +      L+WPD  
Sbjct: 103 LSVEKQVAIALRRLASGDSQVSVGAAFGVGQSTVSQVTWRFIEALEE-RAKHHLRWPDSD 162

Query: 252 KLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGV 311
           ++ +IK +FE + G+P   G+I TTHI +  P    +  +       +Q+ +YS+ +QGV
Sbjct: 163 RIEEIKSKFEEMYGLPNCCGAIDTTHIIMTLPAVQASDDW------CDQEKNYSMFLQGV 222

Query: 312 VDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLND-------------VFIVGNS 371
            D    F ++  GWPG M   ++L+ S  F+      + D              ++VG  
Sbjct: 223 FDHEMRFLNMVTGWPGGMTVSKLLKFSGFFKLCENAQILDGNPKTLSQGAQIREYVVGGI 282

Query: 372 GYPLTDWLLVPYSLQNLTWTQHAFNEKVSEIQAAAKAAFGRLKGRWSCLQK-RTEVKLQE 420
            YPL  WL+ P+   + + +  AFNE+  ++++ A  AF +LKG W  L K       ++
Sbjct: 283 SYPLLPWLITPHDSDHPSDSMVAFNERHEKVRSVAATAFQQLKGSWRILSKVMWRPDRRK 342

BLAST of Lag0034579 vs. ExPASy Swiss-Prot
Match: Q96MB7 (Putative nuclease HARBI1 OS=Homo sapiens OX=9606 GN=HARBI1 PE=1 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 1.4e-17
Identity = 69/261 (26.44%), Postives = 121/261 (46.36%), Query Frame = 0

Query: 164 ELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSA 223
           EL    + + T    AI    +V   +    +G     +    G+  ++  + V  V  A
Sbjct: 51  ELLGANLSRPTQRSRAISPETQVLAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEA 110

Query: 224 IRKVLMPKFLQWP-DDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKR 283
           + +    +F+++P D++ +  +K EF  ++G+P V G +   H+ I AP     +Y N+ 
Sbjct: 111 LVE-RASQFIRFPADEASIQALKDEFYGLAGMPGVMGVVDCIHVAIKAPNAEDLSYVNR- 170

Query: 284 HTERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFI 343
                 K  +S+    V D  G    V   WPGS+ D  VL++S+L  +   G+  D ++
Sbjct: 171 ------KGLHSLNCLMVCDIRGTLMTVETNWPGSLQDCAVLQQSSLSSQFEAGMHKDSWL 230

Query: 344 VGNSGYPLTDWLLVPYSLQNLTWTQHAFNEKVSEIQAAAKAAFGRLKGRWSCLQKRTEVK 403
           +G+S + L  WL+ P  +   T  ++ +N   S   +  +  F  L  R+ CL   ++  
Sbjct: 231 LGDSSFFLRTWLMTPLHIPE-TPAEYRYNMAHSATHSVIEKTFRTLCSRFRCLD-GSKGA 290

Query: 404 LQELPV----VLGACCVLHNI 420
           LQ  P     ++ ACCVLHNI
Sbjct: 291 LQYSPEKSSHIILACCVLHNI 301

BLAST of Lag0034579 vs. ExPASy Swiss-Prot
Match: Q17QR8 (Putative nuclease HARBI1 OS=Bos taurus OX=9913 GN=HARBI1 PE=2 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 5.5e-17
Identity = 68/261 (26.05%), Postives = 120/261 (45.98%), Query Frame = 0

Query: 164 ELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSA 223
           EL    + + T    AI    ++   +    +G     +    G+  ++  + V  V  A
Sbjct: 51  ELLGASLSRPTQRSRAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEA 110

Query: 224 IRKVLMPKFLQWP-DDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKR 283
           + +    +F+ +P D++ +  +K EF  ++GIP V G +   H+ I AP     +Y N+ 
Sbjct: 111 LVE-RASQFIHFPADEASVQALKDEFYGLAGIPGVIGVVDCMHVAIKAPNAEDLSYVNR- 170

Query: 284 HTERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFI 343
                 K  +S+    V D  G    V   WPGS+ D  VL++S+L  +   G+  + ++
Sbjct: 171 ------KGLHSLNCLMVCDIRGALMTVETSWPGSLQDCVVLQQSSLSSQFEAGMHKESWL 230

Query: 344 VGNSGYPLTDWLLVPYSLQNLTWTQHAFNEKVSEIQAAAKAAFGRLKGRWSCLQKRTEVK 403
           +G+S + L  WL+ P  +   T  ++ +N   S   +  +  F  L  R+ CL   ++  
Sbjct: 231 LGDSSFFLRTWLMTPLHIPE-TPAEYRYNMAHSATHSVIEKTFRTLCSRFRCLD-GSKGA 290

Query: 404 LQELPV----VLGACCVLHNI 420
           LQ  P     ++ ACCVLHNI
Sbjct: 291 LQYSPEKSSHIILACCVLHNI 301

BLAST of Lag0034579 vs. ExPASy Swiss-Prot
Match: B0BN95 (Putative nuclease HARBI1 OS=Rattus norvegicus OX=10116 GN=Harbi1 PE=2 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 5.5e-17
Identity = 68/261 (26.05%), Postives = 120/261 (45.98%), Query Frame = 0

Query: 164 ELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSA 223
           EL    + + T    AI    ++   +    +G     +    G+  ++  + V  V  A
Sbjct: 51  ELLGASLSRPTQRSRAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEA 110

Query: 224 IRKVLMPKFLQWP-DDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKR 283
           + +    +F+ +P D++ +  +K EF  ++G+P V G++   H+ I AP     +Y N+ 
Sbjct: 111 LVE-RASQFIHFPADEAAIQSLKDEFYGLAGMPGVIGAVDCIHVAIKAPNAEDLSYVNR- 170

Query: 284 HTERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFI 343
                 K  +S+    V D  G    V   WPGS+ D  VL++S+L  +   G+  D ++
Sbjct: 171 ------KGLHSLNCLVVCDIRGALMTVETSWPGSLQDCAVLQQSSLSSQFETGMPKDSWL 230

Query: 344 VGNSGYPLTDWLLVPYSLQNLTWTQHAFNEKVSEIQAAAKAAFGRLKGRWSCLQKRTEVK 403
           +G+S + L  WLL P  +   T  ++ +N   S   +  +     L  R+ CL   ++  
Sbjct: 231 LGDSSFFLHTWLLTPLHIPE-TPAEYRYNRAHSATHSVIEKTLRTLCCRFRCLD-GSKGA 290

Query: 404 LQELPV----VLGACCVLHNI 420
           LQ  P     ++ ACCVLHNI
Sbjct: 291 LQYSPEKSSHIILACCVLHNI 301

BLAST of Lag0034579 vs. ExPASy TrEMBL
Match: A0A6J1F642 (protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111441167 PE=3 SV=1)

HSP 1 Score: 834.7 bits (2155), Expect = 1.9e-238
Identity = 416/481 (86.49%), Postives = 438/481 (91.06%), Query Frame = 0

Query: 1   MEISSFPFLNQEDLLPIFNLFSEMDNSFSLNQTPKKRRRPEDNGDDH------SQFDGND 60
           MEISSFPFLNQ+DLLPIFNLFSEMD++FS+NQ+PKKRRR  D+ DDH      + FD ++
Sbjct: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNQSPKKRRRQNDDDDDHQTQFNKTSFDDDE 60

Query: 61  LLKLPLWFDTHDDKPDHSWIMDSDADHKREFHLSDESFPQF-SKKPRRETPVSANSSPAK 120
           LLKLP WFD  DDK  H WIM    + K EF +SDE+  QF  KKPRR TP + +SSPAK
Sbjct: 61  LLKLPFWFD--DDKQQH-WIM----EQKPEFQVSDENLTQFVPKKPRRATPENTHSSPAK 120

Query: 121 GGGTGGQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKD 180
            GG G Q RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSK+TFDMIC+ELDSTVMKKD
Sbjct: 121 -GGAGTQHRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKD 180

Query: 181 TMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFL 240
           TMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFL
Sbjct: 181 TMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFL 240

Query: 241 QWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYS 300
           QWP++SKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYS
Sbjct: 241 QWPEESKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYS 300

Query: 301 ITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFIVGNSGYPLTDW 360
           ITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLL DV IVGNSGYPLTDW
Sbjct: 301 ITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDW 360

Query: 361 LLVPYSLQNLTWTQHAFNEKVSEIQAAAKAAFGRLKGRWSCLQKRTEVKLQELPVVLGAC 420
           LLVPYS QNLTWTQHAFNEKVSEIQ AAKAAFGRLKGRW+CLQKRTEVKLQELPVVLGAC
Sbjct: 361 LLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGAC 420

Query: 421 CVLHNICEMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDHIAHNLLHHGLAGTGF 475
           CVLHNICEMRKE FDPEL FE FDDEM+PE+NG+RS SAIQARDHIAHNLLHHGLAGTGF
Sbjct: 421 CVLHNICEMRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGLAGTGF 473

BLAST of Lag0034579 vs. ExPASy TrEMBL
Match: A0A6J1HRQ5 (protein ALP1-like OS=Cucurbita maxima OX=3661 GN=LOC111466889 PE=3 SV=1)

HSP 1 Score: 828.6 bits (2139), Expect = 1.4e-236
Identity = 413/480 (86.04%), Postives = 437/480 (91.04%), Query Frame = 0

Query: 1   MEISSFPFLNQEDLLPIFNLFSEMDNSFSLNQTPKKRRRPEDNGDDHSQ-----FDGNDL 60
           MEISSFPFLNQ+DLLPIFNLFSEMD++FS+N +PKKRRR +D+ DD +Q     FD ++L
Sbjct: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNYSPKKRRRQDDDDDDQTQFNKTSFDDDEL 60

Query: 61  LKLPLWFDTHDDKPDHSWIMDSDADHKREFHLSDESFPQF-SKKPRRETPVSANSSPAKG 120
           LKLP WFD  DDK  H WIM    + K EF + DE+  QF +KKPRR TP + +SSPAK 
Sbjct: 61  LKLPFWFD--DDKQQH-WIM----EQKPEFQVFDENLTQFVAKKPRRATPENTHSSPAK- 120

Query: 121 GGTGGQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDT 180
           GG G Q RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSK+TFDMIC+ELDSTVMKKDT
Sbjct: 121 GGAGTQHRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKDT 180

Query: 181 MLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQ 240
           MLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQ
Sbjct: 181 MLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQ 240

Query: 241 WPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSI 300
           WP+DSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSI
Sbjct: 241 WPEDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSI 300

Query: 301 TVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFIVGNSGYPLTDWL 360
           TVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLL DV IVGNSGYPLTDWL
Sbjct: 301 TVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDWL 360

Query: 361 LVPYSLQNLTWTQHAFNEKVSEIQAAAKAAFGRLKGRWSCLQKRTEVKLQELPVVLGACC 420
           LVPYS QNLTWTQHAFNEKVSEIQ AAKAAFGRLKGRW+CLQKRTEVKLQELPVVLGACC
Sbjct: 361 LVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACC 420

Query: 421 VLHNICEMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDHIAHNLLHHGLAGTGFL 475
           VLHNICEMRKE FDPEL FE FDDEM+PE+NG+RS SAI ARDHI+HNLLHHGLAGTGFL
Sbjct: 421 VLHNICEMRKERFDPELKFEFFDDEMVPENNGVRSASAILARDHISHNLLHHGLAGTGFL 472

BLAST of Lag0034579 vs. ExPASy TrEMBL
Match: A0A6J1HAH8 (protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111461585 PE=3 SV=1)

HSP 1 Score: 817.0 bits (2109), Expect = 4.1e-233
Identity = 403/474 (85.02%), Postives = 426/474 (89.87%), Query Frame = 0

Query: 1   MEISSFPFLNQEDLLPIFNLFSEMDNSFSLNQTPKKRRRPEDNGDDHSQFDGNDLLKLPL 60
           MEI+SFPFLNQ++ LPI N FSEMDN FS+NQ+PKKRRRP DNGDD S   GNDLL  P 
Sbjct: 1   MEITSFPFLNQDEFLPISNFFSEMDN-FSVNQSPKKRRRPTDNGDDGSGNGGNDLLNHPF 60

Query: 61  WFDTHDDKPDHSWIMDSDADHKREFHLSDESFPQFSKKPRRETPVSANSSPAKGGGTGGQ 120
           W +  D++ +H W+MDSD             F   SKKPRR TP + NSSPAK GG G  
Sbjct: 61  WVENRDEQREH-WVMDSD------------EFQVLSKKPRRGTPQNDNSSPAK-GGAGAH 120

Query: 121 QRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAI 180
           QRRLWVK+RSKDWWDQCNHPDFPDEEFRRAFRMSK+TFDMIC+ELDSTVMKKDTMLRVAI
Sbjct: 121 QRRLWVKNRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICRELDSTVMKKDTMLRVAI 180

Query: 181 PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDDSK 240
           PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWP++SK
Sbjct: 181 PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEESK 240

Query: 241 LAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVV 300
           L KIKQEFESISGIPKVGG+IYTTHIPIIAPKN+VAAYFNKRHTERNQKTSYSITVQGVV
Sbjct: 241 LTKIKQEFESISGIPKVGGAIYTTHIPIIAPKNSVAAYFNKRHTERNQKTSYSITVQGVV 300

Query: 301 DPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFIVGNSGYPLTDWLLVPYSL 360
           DPAGVFTDVCIGWPGSMPDD VLEKSALFERANMGLLNDVFIVGNSGYPL DWLLVPY+L
Sbjct: 301 DPAGVFTDVCIGWPGSMPDDHVLEKSALFERANMGLLNDVFIVGNSGYPLMDWLLVPYTL 360

Query: 361 QNLTWTQHAFNEKVSEIQAAAKAAFGRLKGRWSCLQKRTEVKLQELPVVLGACCVLHNIC 420
           QNLTWTQHAFNEKV EIQAAAKAAFGRLKGRW+CLQKRTEVKLQELPVVLGACCVLHNIC
Sbjct: 361 QNLTWTQHAFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNIC 420

Query: 421 EMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDHIAHNLLHHGLAGTGFL 475
           EMRKE FD EL FE+FDDEM+PESNG+RSVSAIQ RDHIAHNLLHHGLAGTGFL
Sbjct: 421 EMRKERFDSELKFEVFDDEMVPESNGVRSVSAIQGRDHIAHNLLHHGLAGTGFL 459

BLAST of Lag0034579 vs. ExPASy TrEMBL
Match: A0A6J1JHN7 (protein ALP1-like OS=Cucurbita maxima OX=3661 GN=LOC111485185 PE=3 SV=1)

HSP 1 Score: 813.9 bits (2101), Expect = 3.5e-232
Identity = 402/474 (84.81%), Postives = 426/474 (89.87%), Query Frame = 0

Query: 1   MEISSFPFLNQEDLLPIFNLFSEMDNSFSLNQTPKKRRRPEDNGDDHSQFDGNDLLKLPL 60
           MEI+S PFLNQ++ LPI N FSE+DN FS+NQ+ KKRRRP DNGDD S   GNDLL LP 
Sbjct: 1   MEITSVPFLNQDEFLPISNFFSEIDN-FSVNQSLKKRRRPTDNGDDGSGNGGNDLLNLPF 60

Query: 61  WFDTHDDKPDHSWIMDSDADHKREFHLSDESFPQFSKKPRRETPVSANSSPAKGGGTGGQ 120
           W +  D++ +H W+MDSD             F   SKKPRR TP + NSSPAK GG G  
Sbjct: 61  WVENRDEQQEH-WVMDSD------------EFQVLSKKPRRGTPQNDNSSPAK-GGAGAH 120

Query: 121 QRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAI 180
           QRRLWVK+RSKDWWDQCNHPDFPDEEFRRAFRMSK+TFDMIC+ELDSTVMKKDTMLRVAI
Sbjct: 121 QRRLWVKNRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICRELDSTVMKKDTMLRVAI 180

Query: 181 PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDDSK 240
           PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWP++SK
Sbjct: 181 PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEESK 240

Query: 241 LAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVV 300
           L KIKQEFESISGIPKVGG+IYTTHIPIIAPKN+VAAYFNKRHTERNQKTSYSITVQGVV
Sbjct: 241 LTKIKQEFESISGIPKVGGAIYTTHIPIIAPKNSVAAYFNKRHTERNQKTSYSITVQGVV 300

Query: 301 DPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFIVGNSGYPLTDWLLVPYSL 360
           DPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFIVGNSGYPL DWLLVPY+L
Sbjct: 301 DPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFIVGNSGYPLMDWLLVPYTL 360

Query: 361 QNLTWTQHAFNEKVSEIQAAAKAAFGRLKGRWSCLQKRTEVKLQELPVVLGACCVLHNIC 420
           QNLTWTQHAFNEKV EIQAAAKAAFGRLKGRW+CLQKRTEVKLQELPVVLGACCVLHNIC
Sbjct: 361 QNLTWTQHAFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNIC 420

Query: 421 EMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDHIAHNLLHHGLAGTGFL 475
           EMRKE FD EL FE+FDDEM+PESNG+RSVSAIQ RDHIAHNLLHHGLAGTGFL
Sbjct: 421 EMRKERFDSELKFEVFDDEMVPESNGVRSVSAIQGRDHIAHNLLHHGLAGTGFL 459

BLAST of Lag0034579 vs. ExPASy TrEMBL
Match: A0A1S3BPN5 (putative nuclease HARBI1 OS=Cucumis melo OX=3656 GN=LOC103492343 PE=3 SV=1)

HSP 1 Score: 810.4 bits (2092), Expect = 3.8e-231
Identity = 406/491 (82.69%), Postives = 432/491 (87.98%), Query Frame = 0

Query: 1   MEISSFPFLNQEDLLPIFNLFSEMDNS----FSLNQTPKKRRRPEDNGDDHSQF---DGN 60
           MEISSFPFLNQE+ LPIFNLFS+MDN+    F++N TPKKRRR + N DD + F   D N
Sbjct: 1   MEISSFPFLNQEEFLPIFNLFSDMDNNPTTPFNVNPTPKKRRRSDPNSDDFNNFSFTDEN 60

Query: 61  D------LLKLPLWFDTHDDKPDHSWIMDSDADH-KREFHLSDESFPQFSKKPRR---ET 120
           D      LLKLP WFD   + P  SW+MDS       +FHLSD    Q  KKPRR   E 
Sbjct: 61  DEPTDDPLLKLPCWFDPQPESP-QSWLMDSQKPKPTNDFHLSD----QIPKKPRRASPEN 120

Query: 121 PVSANSSPAKGGGTGGQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICK 180
           P    ++PA GGGT  QQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICK
Sbjct: 121 PSPVKNNPAGGGGT--QQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICK 180

Query: 181 ELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSA 240
           ELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSA
Sbjct: 181 ELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSA 240

Query: 241 IRKVLMPKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRH 300
           IRKVLMPKFLQWPD+SKL KIKQEFESISGIPKVGGSIYTTHIPIIAP+NNVAAYFNKRH
Sbjct: 241 IRKVLMPKFLQWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPRNNVAAYFNKRH 300

Query: 301 TERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFIV 360
           TERNQKTSYSITVQGVVDP+GVFTDVCIGWPGSMPDDQVLEKS L+ERA+MGLLNDVF+V
Sbjct: 301 TERNQKTSYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGLLNDVFVV 360

Query: 361 GNSGYPLTDWLLVPYSLQNLTWTQHAFNEKVSEIQAAAKAAFGRLKGRWSCLQKRTEVKL 420
           GNSGYPL DWLLVPY++QNLTWTQH FNEKV EIQAAAKAAFGRLKGRW+CLQKRTEVKL
Sbjct: 361 GNSGYPLMDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKL 420

Query: 421 QELPVVLGACCVLHNICEMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDHIAHNL 475
           QELPVVLGACCVLHNICEMRKE FDPEL FE++DDEM+PE+NGLRSVSAIQARDHIAHNL
Sbjct: 421 QELPVVLGACCVLHNICEMRKEKFDPELKFEVYDDEMLPENNGLRSVSAIQARDHIAHNL 480

BLAST of Lag0034579 vs. TAIR 10
Match: AT5G12010.1 (unknown protein; INVOLVED IN: response to salt stress; LOCATED IN: chloroplast, plasma membrane, membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G29780.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 572.8 bits (1475), Expect = 2.6e-163
Identity = 272/376 (72.34%), Postives = 321/376 (85.37%), Query Frame = 0

Query: 105 VSANSSPAKGGGT-----GGQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFD 164
           + A SS   G G+      GQQRRLWVKDRS+ WW++C+  D+P+E+F++AFRMSKSTF+
Sbjct: 128 IEAESSEITGSGSVRGTGSGQQRRLWVKDRSRAWWEECSRLDYPEEDFKKAFRMSKSTFE 187

Query: 165 MICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLE 224
           +IC EL+S V K+DT LR AIPVRQRVAVCIWRLATGEPLRLVSK+FGLGISTCHKLVLE
Sbjct: 188 LICDELNSAVAKEDTALRNAIPVRQRVAVCIWRLATGEPLRLVSKKFGLGISTCHKLVLE 247

Query: 225 VCSAIRKVLMPKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYF 284
           VC AI+ VLMPK+LQWPDD  L  I++ FES+SGIP V GS+YTTHIPIIAPK +VA+YF
Sbjct: 248 VCKAIKDVLMPKYLQWPDDESLRNIRERFESVSGIPNVVGSMYTTHIPIIAPKISVASYF 307

Query: 285 NKRHTERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANM-GLLN 344
           NKRHTERNQKTSYSIT+Q VV+P GVFTD+CIGWPGSMPDD+VLEKS L++RAN  GLL 
Sbjct: 308 NKRHTERNQKTSYSITIQAVVNPKGVFTDLCIGWPGSMPDDKVLEKSLLYQRANNGGLLK 367

Query: 345 DVFIVGNSGYPLTDWLLVPYSLQNLTWTQHAFNEKVSEIQAAAKAAFGRLKGRWSCLQKR 404
            +++ G  G+PL DW+LVPY+ QNLTWTQHAFNEK+SE+Q  AK AFGRLKGRW+CLQKR
Sbjct: 368 GMWVAGGPGHPLLDWVLVPYTQQNLTWTQHAFNEKMSEVQGVAKEAFGRLKGRWACLQKR 427

Query: 405 TEVKLQELPVVLGACCVLHNICEMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDH 464
           TEVKLQ+LP VLGACCVLHNICEMR+E  +PEL  E+ DDE++PE N LRSV+A++ARD 
Sbjct: 428 TEVKLQDLPTVLGACCVLHNICEMREEKMEPELMVEVIDDEVLPE-NVLRSVNAMKARDT 487

Query: 465 IAHNLLHHGLAGTGFL 475
           I+HNLLHHGLAGT FL
Sbjct: 488 ISHNLLHHGLAGTSFL 502

BLAST of Lag0034579 vs. TAIR 10
Match: AT4G29780.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12010.1); Has 945 Blast hits to 944 proteins in 87 species: Archae - 0; Bacteria - 0; Metazoa - 519; Fungi - 43; Plants - 365; Viruses - 0; Other Eukaryotes - 18 (source: NCBI BLink). )

HSP 1 Score: 548.1 bits (1411), Expect = 6.8e-156
Identity = 299/540 (55.37%), Postives = 359/540 (66.48%), Query Frame = 0

Query: 1   MEISSFPF--LNQEDLLPIFNLFSEMD-----------NSFSLNQTPKKRRRPED----- 60
           MEISSFPF  L  ++      LF +MD           NS   N   KKR R +D     
Sbjct: 1   MEISSFPFPYLQDDECSHFLGLFQDMDSSPSTFGLEGFNSNDNNTNQKKRPRKDDEGGGG 60

Query: 61  -----------NGDDHSQFDGNDLLKLPLWFDTHDDKPDHSWIMD-------SDADHKRE 120
                      NG++ + F   D+L   L  D    +    W  +        +A+HK++
Sbjct: 61  GGGGTEVLGAVNGNNKAAF--GDILATLLLLDEEAKQQQEQWDFEFIKEKSLLEANHKKK 120

Query: 121 FHLSDESFPQFS-------------KKPRRETPVSA-------------------NSSPA 180
               D  + Q                K  R+T V+A                    +  A
Sbjct: 121 VKTMDGYYNQMQDHYSAAGETDGSRSKRARKTAVAAVVSAVASGADTTGLAAPVPTADIA 180

Query: 181 KGGGTGGQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKK 240
            G G+G   RRLWVK+R+ DWWD+ + PDFP++EFRR FRMSKSTF++IC+ELD+TV KK
Sbjct: 181 SGSGSGPSHRRLWVKERTTDWWDRVSRPDFPEDEFRREFRMSKSTFNLICEELDTTVTKK 240

Query: 241 DTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKF 300
           +TMLR AIP  +RV VC+WRLATG PLR VS+RFGLGISTCHKLV+EVC AI  VLMPK+
Sbjct: 241 NTMLRDAIPAPKRVGVCVWRLATGAPLRHVSERFGLGISTCHKLVIEVCRAIYDVLMPKY 300

Query: 301 LQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSY 360
           L WP DS++   K +FES+  IP V GSIYTTHIPIIAPK +VAAYFNKRHTERNQKTSY
Sbjct: 301 LLWPSDSEINSTKAKFESVHKIPNVVGSIYTTHIPIIAPKVHVAAYFNKRHTERNQKTSY 360

Query: 361 SITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALF-ERANMGLLNDVFIVGNSGYPLT 420
           SITVQGVV+  G+FTDVCIG PGS+ DDQ+LEKS+L  +RA  G+L D +IVGNSG+PLT
Sbjct: 361 SITVQGVVNADGIFTDVCIGNPGSLTDDQILEKSSLSRQRAARGMLRDSWIVGNSGFPLT 420

Query: 421 DWLLVPYSLQNLTWTQHAFNEKVSEIQAAAKAAFGRLKGRWSCLQKRTEVKLQELPVVLG 472
           D+LLVPY+ QNLTWTQHAFNE + EIQ  A AAF RLKGRW+CLQKRTEVKLQ+LP VLG
Sbjct: 421 DYLLVPYTRQNLTWTQHAFNESIGEIQGIATAAFERLKGRWACLQKRTEVKLQDLPYVLG 480

BLAST of Lag0034579 vs. TAIR 10
Match: AT3G55350.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 160.6 bits (405), Expect = 3.1e-39
Identity = 113/362 (31.22%), Postives = 175/362 (48.34%), Query Frame = 0

Query: 129 RSKDWWDQCNHPDF----PDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVA----I 188
           +S DWWD  +   +      + F   F++S+ TFD IC  + +    K      +    +
Sbjct: 50  QSLDWWDGFSRRIYGGSTDPKTFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPL 109

Query: 189 PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDDSK 248
            +  RVAV + RL +GE L ++ + FG+  ST  ++      ++ +  +   L WP  SK
Sbjct: 110 SLNDRVAVALRRLGSGESLSVIGETFGMNQSTVSQITWRFVESMEERAI-HHLSWP--SK 169

Query: 249 LAKIKQEFESISGIPKVGGSIYTTHI----PIIAPKNNVAAYFNKRHTERNQKTSYSITV 308
           L +IK +FE ISG+P   G+I  THI    P + P N V           + + ++S+T+
Sbjct: 170 LDEIKSKFEKISGLPNCCGAIDITHIVMNLPAVEPSNKVWL---------DGEKNFSMTL 229

Query: 309 QGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMG-LLND------------VFIV 368
           Q VVDP   F DV  GWPGS+ DD VL+ S  ++    G  LN              +IV
Sbjct: 230 QAVVDPDMRFLDVIAGWPGSLNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIV 289

Query: 369 GNSGYPLTDWLLVPYSLQNLTWTQHAFNEKVSEIQAAAKAAFGRLKGRWSCLQKRTEV-K 428
           G+SG+PL  WLL PY  +  +  Q  FN++ SE   AA+ A  +LK RW  +     +  
Sbjct: 290 GDSGFPLLPWLLTPYQGKPTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPD 349

Query: 429 LQELPVVLGACCVLHN-ICEMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDHIAH 464
              LP ++  CC+LHN I +M  ++ D +   +  D      S  L   ++   RD ++ 
Sbjct: 350 RNRLPRIIFVCCLLHNIIIDMEDQTLDDQPLSQQHDMNYRQRSCKLADEASSVLRDELSD 399

BLAST of Lag0034579 vs. TAIR 10
Match: AT3G63270.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: PIF / Ping-Pong family of plant transposases (TAIR:AT3G55350.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 147.5 bits (371), Expect = 2.7e-35
Identity = 94/314 (29.94%), Postives = 156/314 (49.68%), Query Frame = 0

Query: 132 DWWD----QCNHPDFPDEE---FRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAI---- 191
           DWWD    + + P  P +E   F+  FR SK+TF  IC  +   ++ +     + I    
Sbjct: 43  DWWDTFWLRNSSPSVPSDEDYAFKHFFRASKTTFSYICSLVREDLISRPPSGLINIEGRL 102

Query: 192 -PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDDS 251
             V ++VA+ + RLA+G+    V   FG+G ST  ++      A+ +      L+WPD  
Sbjct: 103 LSVEKQVAIALRRLASGDSQVSVGAAFGVGQSTVSQVTWRFIEALEE-RAKHHLRWPDSD 162

Query: 252 KLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGV 311
           ++ +IK +FE + G+P   G+I TTHI +  P    +  +       +Q+ +YS+ +QGV
Sbjct: 163 RIEEIKSKFEEMYGLPNCCGAIDTTHIIMTLPAVQASDDW------CDQEKNYSMFLQGV 222

Query: 312 VDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLND-------------VFIVGNS 371
            D    F ++  GWPG M   ++L+ S  F+      + D              ++VG  
Sbjct: 223 FDHEMRFLNMVTGWPGGMTVSKLLKFSGFFKLCENAQILDGNPKTLSQGAQIREYVVGGI 282

Query: 372 GYPLTDWLLVPYSLQNLTWTQHAFNEKVSEIQAAAKAAFGRLKGRWSCLQK-RTEVKLQE 420
            YPL  WL+ P+   + + +  AFNE+  ++++ A  AF +LKG W  L K       ++
Sbjct: 283 SYPLLPWLITPHDSDHPSDSMVAFNERHEKVRSVAATAFQQLKGSWRILSKVMWRPDRRK 342

BLAST of Lag0034579 vs. TAIR 10
Match: AT3G19120.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 104.4 bits (259), Expect = 2.6e-22
Identity = 80/309 (25.89%), Postives = 142/309 (45.95%), Query Frame = 0

Query: 144 DEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVS 203
           D  +R  + +S   F  +  +L   +    T   +++P    VA+ + RLA G   + ++
Sbjct: 114 DARWRSLYGLSYPVFITVVDKLKPFI----TASNLSLPADYAVAMVLSRLAHGCSAKTLA 173

Query: 204 KRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWP-DDSKLAKIKQEFESISGIPKVGGSIY 263
            R+ L      K+   V   +   L P+F++ P    +L +  Q FE ++ +P + G+I 
Sbjct: 174 SRYSLDPYLISKITNMVTRLLATKLYPEFIKIPVGKRRLIETTQGFEELTSLPNICGAID 233

Query: 264 TTHIPIIAPKNNVAAYFNKRHTERNQKTSY-------SITVQGVVDPAGVFTDVCIGWPG 323
           +T + +            +R T+ N +  Y       ++ +Q V D   +F DVC+  PG
Sbjct: 234 STPVKL------------RRRTKLNPRNIYGCKYGYDAVLLQVVADHKKIFWDVCVKAPG 293

Query: 324 SMPDDQVLEKSALFERANMG------LLN------DVFIVGNSGYPLTDWLLVPYSLQNL 383
              D      S L++R   G      ++N        +IVG+  YPL  +L+ P+S    
Sbjct: 294 GEDDSSHFRDSLLYKRLTSGDIVWEKVINIRGHHVRPYIVGDWCYPLLSFLMTPFSPNGS 353

Query: 384 -TWTQHAFNEKVSEIQAAAKAAFGRLKGRWSCLQKRTEVKLQELPVVLGACCVLHNICEM 432
            T  ++ F+  + + ++    A G LK RW  LQ    V +   P  + ACCVLHN+C++
Sbjct: 354 GTPPENLFDGMLMKGRSVVVEAIGLLKARWKILQS-LNVGVNHAPQTIVACCVLHNLCQI 404