Lag0034465 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0034465 vs. NCBI nr
Match: XP_022144842.1 (E3 ubiquitin-protein ligase ATL42-like [Momordica charantia])

HSP 1 Score: 646.0 bits (1665), Expect = 2.4e-181
Identity = 332/422 (78.67%), Postives = 375/422 (88.86%), Query Frame = 0

Query: 27  MSKFSRIILIIMTTLPFDVNAQAPPPPKPDPGIPQNLNIVIGILSIMVSMAFIFVVYVKF 86
           MSKFSRIILII  TL    NAQ   PPK D GIP+NL ++IGI+SIM+S+AFI V+Y KF
Sbjct: 1   MSKFSRIILIITATLLVHANAQV-SPPKADSGIPENLTMLIGIISIMLSLAFILVLYAKF 60

Query: 87  CHQNPRLRGEPRAHLGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLRGSKKGLECAVCLSK 146
           CH+N R    PRAHLG+GLLRSD RFSGVDQKVIDSLPFFRFSSL+GS++GLECAVCLSK
Sbjct: 61  CHRNSR---GPRAHLGVGLLRSDLRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSK 120

Query: 147 FEDVEVLRLLPQCKHAFHIGCIDHWLENHSSCPLCRCRISVEDIAFFANSNSMRLMRNNS 206
           FED+EVLRLLP+CKHAFHIGC+DHWLENHSSCP+CRCRISVED+AFF  SNSMRLMRNNS
Sbjct: 121 FEDIEVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRISVEDVAFFTCSNSMRLMRNNS 180

Query: 207 QPELPQDPNMELFVQREEDQHNSSRFNIGSSFGKNGKGNKEKEVLIGNDGGECEREEQVL 266
           Q ELPQDPNMELFVQREED HNSSRFNIGSSFGK GKG+KEK+        E + EE V+
Sbjct: 181 QSELPQDPNMELFVQREEDHHNSSRFNIGSSFGKIGKGSKEKD-----GARERQSEEHVV 240

Query: 267 FHKHKHKILVSEFVFVNRWSNLSASDLMFLNTEMLGAMSSNRFSSLDSENIHRSATRETE 326
           FHKHKHKILVS+FVF+NRWSNLS+SDLMFLNTEMLGAMSSNRFSSLD++NI RS T ETE
Sbjct: 241 FHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDTDNIRRSETGETE 300

Query: 327 DRKITQIKEEMEMKRSFERRISIAEKNSLLSISGILSISDSNASTSRATNLHERRSISDT 386
           D++I+ IKEEMEMKRSFE RISIAEKN+L+SI+G+ SISDSNASTSR  NL+ERRS+SDT
Sbjct: 301 DKRISNIKEEMEMKRSFETRISIAEKNTLISITGMPSISDSNASTSRTINLNERRSVSDT 360

Query: 387 TAFSRFSNFNENAIRETSEIEADAKDYRIRQIWLPIAKRTVQWFANREQRSQLTKYTEQP 446
           TAFSRF NFNENAIRETS IE++A+DYRIR+IWLPIA+RTVQWFANRE R+QL+KYT+QP
Sbjct: 361 TAFSRFGNFNENAIRETSVIESNAEDYRIRKIWLPIARRTVQWFANREHRAQLSKYTQQP 413

Query: 447 DV 449
           DV
Sbjct: 421 DV 413

BLAST of Lag0034465 vs. NCBI nr
Match: XP_031738779.1 (putative RING-H2 finger protein ATL12 [Cucumis sativus] >KAE8652892.1 hypothetical protein Csa_022677 [Cucumis sativus])

HSP 1 Score: 636.3 bits (1640), Expect = 1.9e-178
Identity = 330/422 (78.20%), Postives = 367/422 (86.97%), Query Frame = 0

Query: 27  MSKFSRIILIIMTTLPFDVNAQAPPPPKPDPGIPQNLNIVIGILSIMVSMAFIFVVYVKF 86
           M KFSRII+II+T   F V AQAP PPK   GIPQN  IVIGILSIM SM FI VVY KF
Sbjct: 1   MGKFSRIIMIILTIFLFPVIAQAPSPPKAGSGIPQNFTIVIGILSIMFSMVFILVVYAKF 60

Query: 87  CHQNPRLRGEPRAHLGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLRGSKKGLECAVCLSK 146
           CH     RGEP+AHLG GLLRSDSRFSGVDQKVID+LPFFRFSSL+GS++GLECAVCLS 
Sbjct: 61  CHPTSHFRGEPQAHLGHGLLRSDSRFSGVDQKVIDALPFFRFSSLKGSREGLECAVCLSN 120

Query: 147 FEDVEVLRLLPQCKHAFHIGCIDHWLENHSSCPLCRCRISVEDIAFFANSNSMRLMRNNS 206
           FED+EVLRLLP+CKHAFHIGCIDHWLE HSSCP+CRCRI VEDIAFF  SNS+RL+RNNS
Sbjct: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPICRCRIGVEDIAFF-KSNSIRLIRNNS 180

Query: 207 QPELPQDPNMELFVQREEDQHNSSRFNIGSSFGKNGKGNKEKEVLIGNDGGECEREEQVL 266
           + ELPQD  +ELFVQREE+ HNSSR N G+SF   GKGNKEKEVLI N+ GECE EE+VL
Sbjct: 181 RSELPQDSRIELFVQREENHHNSSRSNNGTSFRNIGKGNKEKEVLIENEAGECESEERVL 240

Query: 267 FHKHKHKILVSEFVFVNRWSNLSASDLMFLNTEMLGAMSSNRFSSLDSENIHRSATRETE 326
           FHKHKHKILVS+FVF+NRWSNLS+SDL+FLNTEMLG MSSNRFSSLDSE+I +SATRE E
Sbjct: 241 FHKHKHKILVSDFVFMNRWSNLSSSDLVFLNTEMLGVMSSNRFSSLDSESISQSATREKE 300

Query: 327 DRKITQIKEEMEMKRSFERRISIAEKNSLLSISGILSI-SDSNASTSRATNLHERRSISD 386
            +K +QIKEEMEMKRSFERRISIAEKNSL+SISGI S  SDSNASTSR T L+ERRS+SD
Sbjct: 301 GKKTSQIKEEMEMKRSFERRISIAEKNSLVSISGISSTSSDSNASTSRTTILNERRSVSD 360

Query: 387 TTAFSRFSNFNENAIRETSEIEADAKDYRIRQIWLPIAKRTVQWFANREQRSQLTKYTEQ 446
           TTAFSRF  FNENA R++S IEADAK +RIRQ+WLPIA+RTVQWFANREQR +L+KYT+Q
Sbjct: 361 TTAFSRFGYFNENATRDSSMIEADAKSFRIRQLWLPIARRTVQWFANREQRFRLSKYTQQ 420

Query: 447 PD 448
           PD
Sbjct: 421 PD 421

BLAST of Lag0034465 vs. NCBI nr
Match: XP_031742649.1 (E3 ubiquitin-protein ligase ATL42-like, partial [Cucumis sativus])

HSP 1 Score: 622.9 bits (1605), Expect = 2.2e-174
Identity = 321/406 (79.06%), Postives = 356/406 (87.68%), Query Frame = 0

Query: 43  FDVNAQAPPPPKPDPGIPQNLNIVIGILSIMVSMAFIFVVYVKFCHQNPRLRGEPRAHLG 102
           F V AQAP PPK   GIPQN  IVIGILSIM SM FI VVY KFCH     RGEP+AHLG
Sbjct: 5   FPVIAQAPSPPKAGSGIPQNFTIVIGILSIMFSMVFILVVYAKFCHPTSHFRGEPQAHLG 64

Query: 103 LGLLRSDSRFSGVDQKVIDSLPFFRFSSLRGSKKGLECAVCLSKFEDVEVLRLLPQCKHA 162
            GLLRSDSRFSGVDQKVID+LPFFRFSSL+GS++GLECAVCLSKFED+EVLRLLP+CKHA
Sbjct: 65  HGLLRSDSRFSGVDQKVIDALPFFRFSSLKGSREGLECAVCLSKFEDIEVLRLLPKCKHA 124

Query: 163 FHIGCIDHWLENHSSCPLCRCRISVEDIAFFANSNSMRLMRNNSQPELPQDPNMELFVQR 222
           FHIGCIDHWLE HSSCP+CRCRI VEDIAFF  SNS+RL+RNNS+PELPQD  +ELFVQR
Sbjct: 125 FHIGCIDHWLEKHSSCPICRCRIGVEDIAFF-KSNSIRLIRNNSRPELPQDSRIELFVQR 184

Query: 223 EEDQHNSSRFNIGSSFGKNGKGNKEKEVLIGNDGGECEREEQVLFHKHKHKILVSEFVFV 282
           EE+ HNSSR N G+SF   GKGNKEKEVLI N+ GECE EE+VLFHKHKHKILVS+FVF+
Sbjct: 185 EENHHNSSRSNNGTSFRNIGKGNKEKEVLIENEAGECESEERVLFHKHKHKILVSDFVFM 244

Query: 283 NRWSNLSASDLMFLNTEMLGAMSSNRFSSLDSENIHRSATRETEDRKITQIKEEMEMKRS 342
           NRWSNLS+SDL+FLNTEMLG MSSNRFSSLDSE+I +SATRE E +K +QIKEEMEMKRS
Sbjct: 245 NRWSNLSSSDLVFLNTEMLGVMSSNRFSSLDSESISQSATREKEGKKTSQIKEEMEMKRS 304

Query: 343 FERRISIAEKNSLLSISGILSI-SDSNASTSRATNLHERRSISDTTAFSRFSNFNENAIR 402
           FERRISIAEKNSL+SISGI S  SD NASTSR T L+ERRS+SDTTAFSRF  FNENA R
Sbjct: 305 FERRISIAEKNSLVSISGISSTSSDPNASTSRTTILNERRSVSDTTAFSRFGYFNENATR 364

Query: 403 ETSEIEADAKDYRIRQIWLPIAKRTVQWFANREQRSQLTKYTEQPD 448
           ++S IEADAK +RIRQ+WLPIA+RTVQWFANREQR +L+KYT+QPD
Sbjct: 365 DSSMIEADAKSFRIRQLWLPIARRTVQWFANREQRFRLSKYTQQPD 409

BLAST of Lag0034465 vs. NCBI nr
Match: KAE8647036.1 (hypothetical protein Csa_023000 [Cucumis sativus])

HSP 1 Score: 584.7 bits (1506), Expect = 6.7e-163
Identity = 299/376 (79.52%), Postives = 334/376 (88.83%), Query Frame = 0

Query: 73  MVSMAFIFVVYVKFCHQNPRLRGEPRAHLGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLR 132
           M SM FI VVY KFCH     RGEP+AHLG GLLRSDSRFSGVDQKVID+LPFFRFSSL+
Sbjct: 1   MFSMVFILVVYAKFCHPTSHFRGEPQAHLGHGLLRSDSRFSGVDQKVIDALPFFRFSSLK 60

Query: 133 GSKKGLECAVCLSKFEDVEVLRLLPQCKHAFHIGCIDHWLENHSSCPLCRCRISVEDIAF 192
           GS++GLECAVCLSKFED+EVLRLLP+CKHAFHIGCIDHWLE HSSCP+CRCRI VEDIAF
Sbjct: 61  GSREGLECAVCLSKFEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPICRCRIGVEDIAF 120

Query: 193 FANSNSMRLMRNNSQPELPQDPNMELFVQREEDQHNSSRFNIGSSFGKNGKGNKEKEVLI 252
           F  SNS+RL+RNNS+PELPQD  +ELFVQREE+ HNSSR N G+SF   GKGNKEKEVLI
Sbjct: 121 F-KSNSIRLIRNNSRPELPQDSRIELFVQREENHHNSSRSNNGTSFRNIGKGNKEKEVLI 180

Query: 253 GNDGGECEREEQVLFHKHKHKILVSEFVFVNRWSNLSASDLMFLNTEMLGAMSSNRFSSL 312
            N+ GECE EE+VLFHKHKHKILVS+FVF+NRWSNLS+SDL+FLNTEMLG MSSNRFSSL
Sbjct: 181 ENEAGECESEERVLFHKHKHKILVSDFVFMNRWSNLSSSDLVFLNTEMLGVMSSNRFSSL 240

Query: 313 DSENIHRSATRETEDRKITQIKEEMEMKRSFERRISIAEKNSLLSISGILSI-SDSNAST 372
           DSE+I +SATRE E +K +QIKEEMEMKRSFERRISIAEKNSL+SISGI S  SD NAST
Sbjct: 241 DSESISQSATREKEGKKTSQIKEEMEMKRSFERRISIAEKNSLVSISGISSTSSDPNAST 300

Query: 373 SRATNLHERRSISDTTAFSRFSNFNENAIRETSEIEADAKDYRIRQIWLPIAKRTVQWFA 432
           SR T L+ERRS+SDTTAFSRF  FNENA R++S IEADAK +RIRQ+WLPIA+RTVQWFA
Sbjct: 301 SRTTILNERRSVSDTTAFSRFGYFNENATRDSSMIEADAKSFRIRQLWLPIARRTVQWFA 360

Query: 433 NREQRSQLTKYTEQPD 448
           NREQR +L+KYT+QPD
Sbjct: 361 NREQRFRLSKYTQQPD 375

BLAST of Lag0034465 vs. NCBI nr
Match: KAA0046520.1 (E3 ubiquitin-protein ligase ATL42-like [Cucumis melo var. makuwa] >TYK28739.1 E3 ubiquitin-protein ligase ATL42-like [Cucumis melo var. makuwa])

HSP 1 Score: 529.3 bits (1362), Expect = 3.3e-146
Identity = 287/421 (68.17%), Postives = 328/421 (77.91%), Query Frame = 0

Query: 27  MSKFSRIILIIMTTLPFDVNAQAPPPPKPDPGIPQNLNIVIGILSIMVSMAFIFVVYVKF 86
           M +F+RII+II+    F V AQAP PPK    IPQN  IVIGILSIM SM FI VVY KF
Sbjct: 1   MGRFNRIIMIILIIFLFPVIAQAPSPPKAGSVIPQNFTIVIGILSIMFSMVFILVVYAKF 60

Query: 87  CHQNPRLRGEPRAHLGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLRGSKKGLECAVCLSK 146
           CH     RGEP+A+L  GLLRSDSRFSGVDQKVID+LPFFRFSSL+GS++GLECAVCLSK
Sbjct: 61  CHPTSHFRGEPQANLRHGLLRSDSRFSGVDQKVIDALPFFRFSSLKGSREGLECAVCLSK 120

Query: 147 FEDVEVLRLLPQCKHAFHIGCIDHWLENHSSCPLCRCRISVEDIAFFANSNSMRLMRNNS 206
           FED+EVLRLLP+CKHAFHIGCIDHWLE HSSCP+CRCRISVED+AFF  SNS+RL+RNNS
Sbjct: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPICRCRISVEDVAFF-KSNSIRLIRNNS 180

Query: 207 QPELPQDPNMELFVQREEDQHNSSRFNIGSSFGKNGKGNKEKEVLIGNDGGECEREEQVL 266
           QP+LPQD +MELFV REE+ H SSR N G+SF K GKG+KEKEVLI ++           
Sbjct: 181 QPDLPQDSHMELFVLREENHHYSSRSNDGTSFRKIGKGSKEKEVLIESE----------- 240

Query: 267 FHKHKHKILVSEFVFVNRWSNLSASDLMFLNTEMLGAMSSNRFSSLDSENIHRSATRETE 326
                                           EMLGAMSS+RFSSL+SE+I RSATRE E
Sbjct: 241 -------------------------------AEMLGAMSSSRFSSLESESIRRSATRERE 300

Query: 327 DRKITQIKEEMEMKRSFERRISIAEKNSLLSISGILSISDSNASTSRATNLHERRSISDT 386
            ++ +QIKE MEMKRSFERRISIAEKNSL+SISGI S SDSNASTS+ TNL+E RS+SDT
Sbjct: 301 GKRTSQIKEAMEMKRSFERRISIAEKNSLVSISGISSTSDSNASTSKTTNLNE-RSVSDT 360

Query: 387 TAFSRFSNFNENAIRETSEIEADAKDYRIRQIWLPIAKRTVQWFANREQRSQLTKYTEQP 446
           TAFSRF  FNENAIR++S IEADAK YRIRQ+WLPIA+RTVQWFANRE+RS+L+KYT+QP
Sbjct: 361 TAFSRFGYFNENAIRDSSMIEADAKSYRIRQLWLPIARRTVQWFANREERSRLSKYTQQP 377

Query: 447 D 448
           D
Sbjct: 421 D 377

BLAST of Lag0034465 vs. ExPASy Swiss-Prot
Match: Q5XF85 (E3 ubiquitin-protein ligase ATL42 OS=Arabidopsis thaliana OX=3702 GN=ATL42 PE=1 SV=2)

HSP 1 Score: 303.9 bits (777), Expect = 3.0e-81
Identity = 194/448 (43.30%), Postives = 275/448 (61.38%), Query Frame = 0

Query: 32  RIILIIMTTLPFDVNAQAPPPPKPD---PGIPQNLNIVIGILSIMVSMAFIFVVYVKFCH 91
           +I    +  L    +AQ PPP +          +L +V G+L+IM ++ F+ +VY K CH
Sbjct: 3   QIFFFFLPLLHSYASAQTPPPFRNGDLVANFEPSLAVVTGVLAIMFALTFVLLVYAKCCH 62

Query: 92  ------QNPRLRGEPRAHLGLGLLR---SDSRFSGVDQKVIDSLPFFRFSSLRGSKKGLE 151
                    R R + R   G+   R   S  RFSG+D+  I+SLP FRFS+L+GSK+GL+
Sbjct: 63  IDLRSGSGDRRRHDRRLRQGIFFNRSTASSDRFSGLDKTAIESLPLFRFSALKGSKQGLD 122

Query: 152 CAVCLSKFEDVEVLRLLPQCKHAFHIGCIDHWLENHSSCPLCRCRISV-EDIAFFANSNS 211
           C+VCLSKFE VE+LRLLP+C+HAFHIGCID WLE H++CPLCR R+S+ ED +   N NS
Sbjct: 123 CSVCLSKFESVEILRLLPKCRHAFHIGCIDQWLEQHATCPLCRDRVSMEEDSSVLTNGNS 182

Query: 212 MRLMRNNSQPELPQDPNMELFVQREEDQH--------NSSRFNIGSSFGKNGK-GNKEKE 271
            R +   +Q E+ +D ++EL+++REE++          SSRF+IG SF K  K GNKEK 
Sbjct: 183 FRFL---NQSEIREDSSLELYIEREEEEERIHREELSGSSRFSIGESFRKILKLGNKEKT 242

Query: 272 VLIGNDGGECEREEQVLFHKHKHKILVSEFVFVNRWSNLSASDLMFLNTEMLGAMSSNRF 331
           +L   D    +++E+ L HK  H+I+VS+ VF NRWSN+S+SDLMFLN+EM+ ++SS RF
Sbjct: 243 LL---DEHVNDKDEKKLMHKFNHRIVVSDVVFKNRWSNVSSSDLMFLNSEMVNSISSERF 302

Query: 332 SSLDSENIHRSATRETEDRKITQIKEEMEMKRSFERRISIAEKNSLLSISGILSISDSNA 391
           SSLD    H     E +   I +IKEEME KR  E        N L S++ + S  + ++
Sbjct: 303 SSLD----HVKRGDEEDQIGILRIKEEMEAKRMLE--------NKLTSMTTMFSSENGDS 362

Query: 392 -STSRATNLHE-RRSISDTTAFSRFS-----NFNENAIRETSEIE--ADAKDYRIRQIWL 449
            S SR+  +   RRS+SD TA  R S     + + +A    S ++   +  + R R++WL
Sbjct: 363 GSKSRSVMIEPGRRSVSDITAVPRLSISIHGDCSGSAAETASALQNGGNETEERRRRLWL 422

BLAST of Lag0034465 vs. ExPASy Swiss-Prot
Match: Q9SL78 (Putative RING-H2 finger protein ATL12 OS=Arabidopsis thaliana OX=3702 GN=ATL12 PE=3 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 9.6e-72
Identity = 172/405 (42.47%), Postives = 242/405 (59.75%), Query Frame = 0

Query: 45  VNAQAPPPPK---PDPGIPQNLNIVIGILSIMVSMAFIFVVYVKFCHQNPRLR----GEP 104
           V+AQ+PPPP           +L I+ G+ SI+ ++ F+ +VY K  H + R      GE 
Sbjct: 20  VSAQSPPPPNLYATSDLFKPSLAIITGVFSIVFTLTFVLLVYAKCFHNDLRSETDSDGER 79

Query: 105 RAH--LGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLRGSKKGLECAVCLSKFEDVEVLRL 164
             H  L  GL    SRFSG+D+K I+SLPFFRFS+L+G K+GLEC+VCLSKFEDVE+LRL
Sbjct: 80  IRHDRLWQGLFNRSSRFSGLDKKAIESLPFFRFSALKGLKQGLECSVCLSKFEDVEILRL 139

Query: 165 LPQCKHAFHIGCIDHWLENHSSCPLCRCRISVEDIAFFANSNSMRLMRNNSQPELPQDPN 224
           LP+C+HAFHIGCID WLE H++CPLCR R+++ED      ++S  L   N      +D  
Sbjct: 140 LPKCRHAFHIGCIDQWLEQHATCPLCRNRVNIEDDLSVLGNSSTSLRILNQSETREEDSR 199

Query: 225 MELFVQREEDQHN-SSRFNIGSSFGKNGKGNKEKEVLIGNDGGECEREEQVLFHKHKHKI 284
           +E++++REE  ++ SSRF   SSF K  K    K +L+  +G E   +E+ L HK  H+I
Sbjct: 200 LEIYIEREEGTNDGSSRF---SSFRKILK----KSLLLEREGNE-NIDEKKLMHKFNHRI 259

Query: 285 LVSEFVFVNRWSNLSASDLMFLNTEMLGAMSSNRFSSLDSENIHRSATRETEDRKITQIK 344
           +VS+ VF NRWSN+++SDL FL +EML ++SS+RFSS+D   +HR   R+         K
Sbjct: 260 VVSDAVFKNRWSNITSSDLTFLTSEMLNSVSSDRFSSVD--RVHRGNLRD---------K 319

Query: 345 EEMEMKRSFERRISIAEKNSLLSISGILSISDSNASTSRATNLHERRSISDTTAFSRFSN 404
           E+MEMK     R+ I  K+S            S  + S  T +   +++  +   S  S 
Sbjct: 320 EDMEMK-----RMLIKHKDS------------SRRTVSEITTVSREKAVGGSYRGSTAST 379

Query: 405 FNENAIRETSEIEADAKDYRIRQIWLPIAKRTVQWFANREQRSQL 440
               A+  T+E        R R++WLPIA+RT QWF NRE+ + L
Sbjct: 380 SQNYAVTATTE-------ERRRRLWLPIARRTAQWFVNREKSNDL 381

BLAST of Lag0034465 vs. ExPASy Swiss-Prot
Match: Q5EAE9 (RING-H2 finger protein ATL43 OS=Arabidopsis thaliana OX=3702 GN=ATL43 PE=2 SV=2)

HSP 1 Score: 148.3 bits (373), Expect = 2.1e-34
Identity = 106/348 (30.46%), Postives = 171/348 (49.14%), Query Frame = 0

Query: 8   SSKGSVRVEKSFSRVFNFSMSKFSRIILIIMTTLPFDVNAQAPPPPKPDP------GIPQ 67
           SS   + +  + S   N S++     ++I  +  P  +     PPP P P       +  
Sbjct: 2   SSSSLILLFSTLSLFLNVSLADNHTAVVITTSDTPPPL-----PPPSPPPRHNFTSSLMP 61

Query: 68  NLNIVIGILSIMVSMAFIFVVYVKFCHQ--------NPRLRGEPR---AHLGLGLLRSDS 127
            + +VI +L+   S+ F+ ++YVK C +        +P+     R    +   G +    
Sbjct: 62  GIAVVIAVLTAFFSLTFLLLLYVKHCKRRNGSVYVNHPQRFAITRYGGGYYNGGGVVGGR 121

Query: 128 RFSGVDQKVIDSLPFFRFSSLRGSKKGLECAVCLSKFEDVEVLRLLPQCKHAFHIGCIDH 187
           + SG+D+ VI+SLP FRF +L G K GLECAVCL++FE  EVLRLLP+CKHAFH+ C+D 
Sbjct: 122 KNSGIDRSVIESLPVFRFGALSGHKDGLECAVCLARFEPTEVLRLLPKCKHAFHVECVDT 181

Query: 188 WLENHSSCPLCRCRISVEDIAFFANSNS---MRLMRNNSQPELPQDPNMELF--VQREED 247
           WL+ HS+CPLCR R+  EDI    + NS   ++  ++ S       P +  F  V R   
Sbjct: 182 WLDAHSTCPLCRYRVDPEDILLIGDCNSWFELQFSKDESNSVNNNPPGLTRFIPVSRISG 241

Query: 248 QHNSS-----------------------RFNIGSSFGKNGKGNKEKEVLIGNDGGECERE 302
           +H+S+                       R ++ SS   N  G ++ E +  N     +R+
Sbjct: 242 RHSSAGERASRLNEIRTSSSYKSNPMSFRRSLDSSLKVNDAGEEKSESVAVNCLDRLQRK 301

BLAST of Lag0034465 vs. ExPASy Swiss-Prot
Match: Q8W571 (RING-H2 finger protein ATL32 OS=Arabidopsis thaliana OX=3702 GN=ATL32 PE=2 SV=3)

HSP 1 Score: 105.5 bits (262), Expect = 1.6e-21
Identity = 59/164 (35.98%), Postives = 89/164 (54.27%), Query Frame = 0

Query: 33  IILIIMTTLPFDVNAQ--APPPPKPDPGIPQNLNIVIGILSIMVSMAFIFVVYVKFCHQN 92
           IIL +   +    NAQ  +P PP    G   +   V  +L  +  +  +  VY++ C   
Sbjct: 14  IILHVAIIIQSKANAQSFSPSPPDLQTGHTPSKTTVFAVLVTLFFLTGLLSVYIRHC--- 73

Query: 93  PRLRGEPRAHLGLGLLRSD---SRFSGVDQKVIDSLPFFRFSSLRGSK---KGLECAVCL 152
              R  P +       R++   SR  G+D  V++S P F +SS++ SK   K LECA+CL
Sbjct: 74  --ARSNPDSSTRYFRNRANDGSSRRGGLDNAVVESFPVFAYSSVKESKIGSKDLECAICL 133

Query: 153 SKFEDVEVLRLLPQCKHAFHIGCIDHWLENHSSCPLCRCRISVE 189
           ++ ED E +RLLP C H FHI CID WL +H++CP+CR  ++ +
Sbjct: 134 NELEDHETVRLLPICNHLFHIDCIDTWLYSHATCPVCRSNLTAK 172

BLAST of Lag0034465 vs. ExPASy Swiss-Prot
Match: Q9ZV53 (Putative RING-H2 finger protein ATL49 OS=Arabidopsis thaliana OX=3702 GN=ATL49 PE=3 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 1.9e-19
Identity = 69/201 (34.33%), Postives = 99/201 (49.25%), Query Frame = 0

Query: 47  AQAPPPP-----------KPDPGIPQNLNIVIGILSIMVSMAFIFVVYVKFCHQNPRLRG 106
           + +PPPP             +  I  N+ ++I ILSI+  ++ +  + VKF     R   
Sbjct: 16  SSSPPPPLIPLKSNTSLSNLNSKITPNILLIIIILSIIFFISGLLHILVKFLLTPSRESR 75

Query: 107 EP-----RAHLGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLRGSK-KGLECAVCLSKFED 166
           E       A  G      +   SGVDQ +ID+LP F + S+ G K    +C VCL +FE 
Sbjct: 76  EDYFDNVTALQGQLQQLFNLHDSGVDQSLIDTLPVFHYKSIVGLKISPFDCPVCLCEFET 135

Query: 167 VEVLRLLPQCKHAFHIGCIDHWLENHSSCPLCRCRISVEDIAFFANSNSMRLMRNNSQPE 226
            + LRLLP+C HAFH+ CID WL +HS+CPLCR  +     +    S+S  L+  + Q  
Sbjct: 136 EDKLRLLPKCSHAFHVECIDTWLLSHSTCPLCRSNLLSGFSSHHNLSSSYLLVLESEQSS 195

Query: 227 LPQDPNMELFVQREEDQHNSS 231
               P +E   Q   D +N S
Sbjct: 196 RDMVPVLESNSQLGYDVNNDS 216

BLAST of Lag0034465 vs. ExPASy TrEMBL
Match: A0A6J1CUV0 (E3 ubiquitin-protein ligase ATL42-like OS=Momordica charantia OX=3673 GN=LOC111014428 PE=4 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 1.2e-181
Identity = 332/422 (78.67%), Postives = 375/422 (88.86%), Query Frame = 0

Query: 27  MSKFSRIILIIMTTLPFDVNAQAPPPPKPDPGIPQNLNIVIGILSIMVSMAFIFVVYVKF 86
           MSKFSRIILII  TL    NAQ   PPK D GIP+NL ++IGI+SIM+S+AFI V+Y KF
Sbjct: 1   MSKFSRIILIITATLLVHANAQV-SPPKADSGIPENLTMLIGIISIMLSLAFILVLYAKF 60

Query: 87  CHQNPRLRGEPRAHLGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLRGSKKGLECAVCLSK 146
           CH+N R    PRAHLG+GLLRSD RFSGVDQKVIDSLPFFRFSSL+GS++GLECAVCLSK
Sbjct: 61  CHRNSR---GPRAHLGVGLLRSDLRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSK 120

Query: 147 FEDVEVLRLLPQCKHAFHIGCIDHWLENHSSCPLCRCRISVEDIAFFANSNSMRLMRNNS 206
           FED+EVLRLLP+CKHAFHIGC+DHWLENHSSCP+CRCRISVED+AFF  SNSMRLMRNNS
Sbjct: 121 FEDIEVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRISVEDVAFFTCSNSMRLMRNNS 180

Query: 207 QPELPQDPNMELFVQREEDQHNSSRFNIGSSFGKNGKGNKEKEVLIGNDGGECEREEQVL 266
           Q ELPQDPNMELFVQREED HNSSRFNIGSSFGK GKG+KEK+        E + EE V+
Sbjct: 181 QSELPQDPNMELFVQREEDHHNSSRFNIGSSFGKIGKGSKEKD-----GARERQSEEHVV 240

Query: 267 FHKHKHKILVSEFVFVNRWSNLSASDLMFLNTEMLGAMSSNRFSSLDSENIHRSATRETE 326
           FHKHKHKILVS+FVF+NRWSNLS+SDLMFLNTEMLGAMSSNRFSSLD++NI RS T ETE
Sbjct: 241 FHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDTDNIRRSETGETE 300

Query: 327 DRKITQIKEEMEMKRSFERRISIAEKNSLLSISGILSISDSNASTSRATNLHERRSISDT 386
           D++I+ IKEEMEMKRSFE RISIAEKN+L+SI+G+ SISDSNASTSR  NL+ERRS+SDT
Sbjct: 301 DKRISNIKEEMEMKRSFETRISIAEKNTLISITGMPSISDSNASTSRTINLNERRSVSDT 360

Query: 387 TAFSRFSNFNENAIRETSEIEADAKDYRIRQIWLPIAKRTVQWFANREQRSQLTKYTEQP 446
           TAFSRF NFNENAIRETS IE++A+DYRIR+IWLPIA+RTVQWFANRE R+QL+KYT+QP
Sbjct: 361 TAFSRFGNFNENAIRETSVIESNAEDYRIRKIWLPIARRTVQWFANREHRAQLSKYTQQP 413

Query: 447 DV 449
           DV
Sbjct: 421 DV 413

BLAST of Lag0034465 vs. ExPASy TrEMBL
Match: A0A5D3DZA7 (E3 ubiquitin-protein ligase ATL42-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00780 PE=4 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 1.6e-146
Identity = 287/421 (68.17%), Postives = 328/421 (77.91%), Query Frame = 0

Query: 27  MSKFSRIILIIMTTLPFDVNAQAPPPPKPDPGIPQNLNIVIGILSIMVSMAFIFVVYVKF 86
           M +F+RII+II+    F V AQAP PPK    IPQN  IVIGILSIM SM FI VVY KF
Sbjct: 1   MGRFNRIIMIILIIFLFPVIAQAPSPPKAGSVIPQNFTIVIGILSIMFSMVFILVVYAKF 60

Query: 87  CHQNPRLRGEPRAHLGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLRGSKKGLECAVCLSK 146
           CH     RGEP+A+L  GLLRSDSRFSGVDQKVID+LPFFRFSSL+GS++GLECAVCLSK
Sbjct: 61  CHPTSHFRGEPQANLRHGLLRSDSRFSGVDQKVIDALPFFRFSSLKGSREGLECAVCLSK 120

Query: 147 FEDVEVLRLLPQCKHAFHIGCIDHWLENHSSCPLCRCRISVEDIAFFANSNSMRLMRNNS 206
           FED+EVLRLLP+CKHAFHIGCIDHWLE HSSCP+CRCRISVED+AFF  SNS+RL+RNNS
Sbjct: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPICRCRISVEDVAFF-KSNSIRLIRNNS 180

Query: 207 QPELPQDPNMELFVQREEDQHNSSRFNIGSSFGKNGKGNKEKEVLIGNDGGECEREEQVL 266
           QP+LPQD +MELFV REE+ H SSR N G+SF K GKG+KEKEVLI ++           
Sbjct: 181 QPDLPQDSHMELFVLREENHHYSSRSNDGTSFRKIGKGSKEKEVLIESE----------- 240

Query: 267 FHKHKHKILVSEFVFVNRWSNLSASDLMFLNTEMLGAMSSNRFSSLDSENIHRSATRETE 326
                                           EMLGAMSS+RFSSL+SE+I RSATRE E
Sbjct: 241 -------------------------------AEMLGAMSSSRFSSLESESIRRSATRERE 300

Query: 327 DRKITQIKEEMEMKRSFERRISIAEKNSLLSISGILSISDSNASTSRATNLHERRSISDT 386
            ++ +QIKE MEMKRSFERRISIAEKNSL+SISGI S SDSNASTS+ TNL+E RS+SDT
Sbjct: 301 GKRTSQIKEAMEMKRSFERRISIAEKNSLVSISGISSTSDSNASTSKTTNLNE-RSVSDT 360

Query: 387 TAFSRFSNFNENAIRETSEIEADAKDYRIRQIWLPIAKRTVQWFANREQRSQLTKYTEQP 446
           TAFSRF  FNENAIR++S IEADAK YRIRQ+WLPIA+RTVQWFANRE+RS+L+KYT+QP
Sbjct: 361 TAFSRFGYFNENAIRDSSMIEADAKSYRIRQLWLPIARRTVQWFANREERSRLSKYTQQP 377

Query: 447 D 448
           D
Sbjct: 421 D 377

BLAST of Lag0034465 vs. ExPASy TrEMBL
Match: A0A1S3CHH4 (E3 ubiquitin-protein ligase ATL42-like OS=Cucumis melo OX=3656 GN=LOC103500996 PE=4 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 1.3e-140
Identity = 279/421 (66.27%), Postives = 317/421 (75.30%), Query Frame = 0

Query: 27  MSKFSRIILIIMTTLPFDVNAQAPPPPKPDPGIPQNLNIVIGILSIMVSMAFIFVVYVKF 86
           M +F+RII+II+    F V AQAP PPK    IPQN  IVIGILSIM SM FI VVY KF
Sbjct: 1   MGRFNRIIMIILIIFLFPVIAQAPSPPKAGSVIPQNFTIVIGILSIMFSMVFILVVYAKF 60

Query: 87  CHQNPRLRGEPRAHLGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLRGSKKGLECAVCLSK 146
           CH     RGEP+A+L  GLLRSDSRFSGVDQKVID+LPFFRFSSL+GS++GLECAVCLSK
Sbjct: 61  CHPTSHFRGEPQANLRHGLLRSDSRFSGVDQKVIDALPFFRFSSLKGSREGLECAVCLSK 120

Query: 147 FEDVEVLRLLPQCKHAFHIGCIDHWLENHSSCPLCRCRISVEDIAFFANSNSMRLMRNNS 206
           FED+EVLRLLP+CKHAFHIGCIDHWLE HSSCP+CRCRISVED+AFF  SNS+RL+RNNS
Sbjct: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPICRCRISVEDVAFF-KSNSIRLIRNNS 180

Query: 207 QPELPQDPNMELFVQREEDQHNSSRFNIGSSFGKNGKGNKEKEVLIGNDGGECEREEQVL 266
           QP+LPQD +MELFV REE+ H SSR N G+SF K GK                       
Sbjct: 181 QPDLPQDSHMELFVLREENHHYSSRSNDGTSFRKIGK----------------------- 240

Query: 267 FHKHKHKILVSEFVFVNRWSNLSASDLMFLNTEMLGAMSSNRFSSLDSENIHRSATRETE 326
                                           EMLGAMSS+RFSSL+SE+I RSATRE E
Sbjct: 241 --------------------------------EMLGAMSSSRFSSLESESIRRSATRERE 300

Query: 327 DRKITQIKEEMEMKRSFERRISIAEKNSLLSISGILSISDSNASTSRATNLHERRSISDT 386
            ++ +QIKE MEMKRSFERRISIAEKNSL+SISGI S SDSNASTS+ TNL+E RS+SDT
Sbjct: 301 GKRTSQIKEAMEMKRSFERRISIAEKNSLVSISGISSTSDSNASTSKTTNLNE-RSVSDT 360

Query: 387 TAFSRFSNFNENAIRETSEIEADAKDYRIRQIWLPIAKRTVQWFANREQRSQLTKYTEQP 446
           TAFSRF  FNENAIR++S IEADAK YRIRQ+WLPIA+RTVQWFANRE+RS+L+KYT+QP
Sbjct: 361 TAFSRFGYFNENAIRDSSMIEADAKSYRIRQLWLPIARRTVQWFANREERSRLSKYTQQP 364

Query: 447 D 448
           D
Sbjct: 421 D 364

BLAST of Lag0034465 vs. ExPASy TrEMBL
Match: A0A7N2KVY2 (RING-type domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 4.2e-110
Identity = 224/417 (53.72%), Postives = 295/417 (70.74%), Query Frame = 0

Query: 34  ILIIMTTLPFDVNAQAPPPPKPDPGIPQNLNIVIGILSIMVSMAFIFVVYVKFCHQNPRL 93
           +++   ++  D NA         P +  +L +VIGILSIM S+ F+ +VY KFCH+   +
Sbjct: 4   VIVSADSVVDDSNADGDNQAASQP-VQPSLAVVIGILSIMFSLTFLLLVYAKFCHRRGSI 63

Query: 94  RGEPRAHLGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLRGSKKGLECAVCLSKFEDVEVL 153
             +P+     G +RS SRFSG+D+ VI+SLPFFRFSSL+G+K+GLEC+VCLSKFED E+L
Sbjct: 64  HSDPQ---NFGFIRSSSRFSGIDKTVIESLPFFRFSSLKGTKEGLECSVCLSKFEDTEML 123

Query: 154 RLLPQCKHAFHIGCIDHWLENHSSCPLCRCRISVEDIAFFANSNSMRLMRNNSQPELPQD 213
           RLLP+CKHAFHI CID+WL  HSSCPLCR ++S ED   F  +NSMRLM   +Q EL QD
Sbjct: 124 RLLPKCKHAFHIDCIDNWLIKHSSCPLCRHKVSPEDPTIFTYTNSMRLM---NQSELRQD 183

Query: 214 PNMELFVQREEDQHNSSRFNIGSSFGKNGKGNKEKEVLIGNDGGECEREEQVLFHKHKHK 273
            N+ELFVQREED H SSRF+IGSSF K  KGN E++ LI  +  +   E   + HKH HK
Sbjct: 184 SNIELFVQREEDHHGSSRFSIGSSFRKTEKGNTEEKPLIFQEEADNNDENNKVLHKHNHK 243

Query: 274 ILVSEFVFVNRWSNLSASDLMFLNTEMLGAMSSNRFSSLDSENIHRSATRETEDRKITQI 333
           I+VS+F+F NRWS++S+SDLMFLN+E+L A SS RFSSLD  N   S  R  E+  I +I
Sbjct: 244 IIVSDFIFNNRWSSVSSSDLMFLNSELLSATSSYRFSSLDWNNEQSSTPRSLENGHIVKI 303

Query: 334 KEEMEMKRSFERRISIAEKNSLLSISGILSISDSN---ASTSRATNLHERRSISDTTAFS 393
           KEEME+KRSFE ++S   K S +S  G+ S SDS    + TSR  N  ERRS+S+ TA S
Sbjct: 304 KEEMELKRSFETKVSTLNKTSPVSFPGLPSTSDSTPNLSQTSRYMNPTERRSMSEITALS 363

Query: 394 RFSNFN--ENAIRETSEIEADAKDYRIRQIWLPIAKRTVQWFANREQRSQLTKYTEQ 446
           R+ + +  +N IRE+S IE + K  R+R++W PIAKRTV+W+ANRE+RSQ ++ T++
Sbjct: 364 RYEDISGIKNRIRESSLIENNVKAERMRRLWFPIAKRTVKWYANRERRSQQSQNTQR 413

BLAST of Lag0034465 vs. ExPASy TrEMBL
Match: A0A2N9GBZ7 (RING-type domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS24653 PE=4 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 1.2e-109
Identity = 224/417 (53.72%), Postives = 291/417 (69.78%), Query Frame = 0

Query: 33  IILIIMTTLPFDVNAQAPPPPKPDPGIPQNLNIVIGILSIMVSMAFIFVVYVKFCHQNPR 92
           +++ ++    F V AQ        PG   +L +VIGILS+M S+  + +VY KFCH+   
Sbjct: 6   LLIFVLHFFFFHVKAQYTGDGSASPG---SLTVVIGILSLMFSLTILLLVYAKFCHRRGP 65

Query: 93  LRGEPRAHLGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLRGSKKGLECAVCLSKFEDVEV 152
           +  +P+      L+RS SRFSG+D+ VI+SLPFFRFSSL+GS+ GLECAVCLSKFED E+
Sbjct: 66  IHSDPQ---NFALIRSSSRFSGIDKTVIESLPFFRFSSLKGSRDGLECAVCLSKFEDTEI 125

Query: 153 LRLLPQCKHAFHIGCIDHWLENHSSCPLCRCRISVEDIAFFANSNSMRLMRNNSQPELPQ 212
           LRLLP+CKHAFHI CID+WLE H+SCPLCR ++S ED + F  +NSMRL+   +Q EL Q
Sbjct: 126 LRLLPKCKHAFHIECIDNWLEKHASCPLCRHKVSAEDPSIFTYTNSMRLI---NQSELRQ 185

Query: 213 DPNMELFVQREEDQHNSSRFNIGSSFGKNGKGNKEKEVLIGNDGGECEREEQVLFHKHKH 272
           D N+ELFVQREED H SSRF+IG+SF K  KG+KE+  LI  +      E  ++ HKH H
Sbjct: 186 DSNIELFVQREEDHHGSSRFSIGNSFRKTEKGDKEENPLIQEEADNSFDENHIVLHKHNH 245

Query: 273 KILVSEFVFVNRWSNLSASDLMFLNTEMLGAMSSNRFSSLDSENIHRSATRETEDRKITQ 332
           KI+V +FVF NRWSNLS+SDLMFLN+EMLGA SS RF+S D  N   S  RE E   I +
Sbjct: 246 KIIVPDFVFKNRWSNLSSSDLMFLNSEMLGATSSYRFASFDLNNEQSSTARENE--HIVK 305

Query: 333 IKEEMEMKRSFERRISIAEKNSLLSISGILSISDSNAS---TSRATNLHERRSISDTTAF 392
           IKEEM+MKR FE ++S   K S +S  GI S SDS ++   TSR  N  ERRS+S+ TA 
Sbjct: 306 IKEEMDMKRLFENKVSTLNKTSAVSFPGIPSTSDSTSNLSQTSRYMNPTERRSMSEITAL 365

Query: 393 SRFSNFN-ENAIRETSEIEADAKDYRIRQIWLPIAKRTVQWFANREQRSQLTKYTEQ 446
           SR+ +   +N I E S +  + K  R+R++WLPIA+RTV W+ANRE+RSQ ++ T+Q
Sbjct: 366 SRYEDSGMKNRIGEASLLGNNVKAERMRRLWLPIARRTVHWYANRERRSQQSQNTQQ 411

BLAST of Lag0034465 vs. TAIR 10
Match: AT4G28890.1 (RING/U-box superfamily protein )

HSP 1 Score: 303.9 bits (777), Expect = 2.1e-82
Identity = 194/448 (43.30%), Postives = 275/448 (61.38%), Query Frame = 0

Query: 32  RIILIIMTTLPFDVNAQAPPPPKPD---PGIPQNLNIVIGILSIMVSMAFIFVVYVKFCH 91
           +I    +  L    +AQ PPP +          +L +V G+L+IM ++ F+ +VY K CH
Sbjct: 3   QIFFFFLPLLHSYASAQTPPPFRNGDLVANFEPSLAVVTGVLAIMFALTFVLLVYAKCCH 62

Query: 92  ------QNPRLRGEPRAHLGLGLLR---SDSRFSGVDQKVIDSLPFFRFSSLRGSKKGLE 151
                    R R + R   G+   R   S  RFSG+D+  I+SLP FRFS+L+GSK+GL+
Sbjct: 63  IDLRSGSGDRRRHDRRLRQGIFFNRSTASSDRFSGLDKTAIESLPLFRFSALKGSKQGLD 122

Query: 152 CAVCLSKFEDVEVLRLLPQCKHAFHIGCIDHWLENHSSCPLCRCRISV-EDIAFFANSNS 211
           C+VCLSKFE VE+LRLLP+C+HAFHIGCID WLE H++CPLCR R+S+ ED +   N NS
Sbjct: 123 CSVCLSKFESVEILRLLPKCRHAFHIGCIDQWLEQHATCPLCRDRVSMEEDSSVLTNGNS 182

Query: 212 MRLMRNNSQPELPQDPNMELFVQREEDQH--------NSSRFNIGSSFGKNGK-GNKEKE 271
            R +   +Q E+ +D ++EL+++REE++          SSRF+IG SF K  K GNKEK 
Sbjct: 183 FRFL---NQSEIREDSSLELYIEREEEEERIHREELSGSSRFSIGESFRKILKLGNKEKT 242

Query: 272 VLIGNDGGECEREEQVLFHKHKHKILVSEFVFVNRWSNLSASDLMFLNTEMLGAMSSNRF 331
           +L   D    +++E+ L HK  H+I+VS+ VF NRWSN+S+SDLMFLN+EM+ ++SS RF
Sbjct: 243 LL---DEHVNDKDEKKLMHKFNHRIVVSDVVFKNRWSNVSSSDLMFLNSEMVNSISSERF 302

Query: 332 SSLDSENIHRSATRETEDRKITQIKEEMEMKRSFERRISIAEKNSLLSISGILSISDSNA 391
           SSLD    H     E +   I +IKEEME KR  E        N L S++ + S  + ++
Sbjct: 303 SSLD----HVKRGDEEDQIGILRIKEEMEAKRMLE--------NKLTSMTTMFSSENGDS 362

Query: 392 -STSRATNLHE-RRSISDTTAFSRFS-----NFNENAIRETSEIE--ADAKDYRIRQIWL 449
            S SR+  +   RRS+SD TA  R S     + + +A    S ++   +  + R R++WL
Sbjct: 363 GSKSRSVMIEPGRRSVSDITAVPRLSISIHGDCSGSAAETASALQNGGNETEERRRRLWL 422

BLAST of Lag0034465 vs. TAIR 10
Match: AT2G20030.1 (RING/U-box superfamily protein )

HSP 1 Score: 272.3 bits (695), Expect = 6.8e-73
Identity = 172/405 (42.47%), Postives = 242/405 (59.75%), Query Frame = 0

Query: 45  VNAQAPPPPK---PDPGIPQNLNIVIGILSIMVSMAFIFVVYVKFCHQNPRLR----GEP 104
           V+AQ+PPPP           +L I+ G+ SI+ ++ F+ +VY K  H + R      GE 
Sbjct: 20  VSAQSPPPPNLYATSDLFKPSLAIITGVFSIVFTLTFVLLVYAKCFHNDLRSETDSDGER 79

Query: 105 RAH--LGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLRGSKKGLECAVCLSKFEDVEVLRL 164
             H  L  GL    SRFSG+D+K I+SLPFFRFS+L+G K+GLEC+VCLSKFEDVE+LRL
Sbjct: 80  IRHDRLWQGLFNRSSRFSGLDKKAIESLPFFRFSALKGLKQGLECSVCLSKFEDVEILRL 139

Query: 165 LPQCKHAFHIGCIDHWLENHSSCPLCRCRISVEDIAFFANSNSMRLMRNNSQPELPQDPN 224
           LP+C+HAFHIGCID WLE H++CPLCR R+++ED      ++S  L   N      +D  
Sbjct: 140 LPKCRHAFHIGCIDQWLEQHATCPLCRNRVNIEDDLSVLGNSSTSLRILNQSETREEDSR 199

Query: 225 MELFVQREEDQHN-SSRFNIGSSFGKNGKGNKEKEVLIGNDGGECEREEQVLFHKHKHKI 284
           +E++++REE  ++ SSRF   SSF K  K    K +L+  +G E   +E+ L HK  H+I
Sbjct: 200 LEIYIEREEGTNDGSSRF---SSFRKILK----KSLLLEREGNE-NIDEKKLMHKFNHRI 259

Query: 285 LVSEFVFVNRWSNLSASDLMFLNTEMLGAMSSNRFSSLDSENIHRSATRETEDRKITQIK 344
           +VS+ VF NRWSN+++SDL FL +EML ++SS+RFSS+D   +HR   R+         K
Sbjct: 260 VVSDAVFKNRWSNITSSDLTFLTSEMLNSVSSDRFSSVD--RVHRGNLRD---------K 319

Query: 345 EEMEMKRSFERRISIAEKNSLLSISGILSISDSNASTSRATNLHERRSISDTTAFSRFSN 404
           E+MEMK     R+ I  K+S            S  + S  T +   +++  +   S  S 
Sbjct: 320 EDMEMK-----RMLIKHKDS------------SRRTVSEITTVSREKAVGGSYRGSTAST 379

Query: 405 FNENAIRETSEIEADAKDYRIRQIWLPIAKRTVQWFANREQRSQL 440
               A+  T+E        R R++WLPIA+RT QWF NRE+ + L
Sbjct: 380 SQNYAVTATTE-------ERRRRLWLPIARRTAQWFVNREKSNDL 381

BLAST of Lag0034465 vs. TAIR 10
Match: AT5G05810.1 (RING/U-box superfamily protein )

HSP 1 Score: 143.3 bits (360), Expect = 4.8e-34
Identity = 97/293 (33.11%), Postives = 151/293 (51.54%), Query Frame = 0

Query: 57  PGIPQNLNIVIGILSIMVSMAFIFVVYVKFCHQ--------NPRLRGEPR---AHLGLGL 116
           PGI     +VI +L+   S+ F+ ++YVK C +        +P+     R    +   G 
Sbjct: 2   PGIA----VVIAVLTAFFSLTFLLLLYVKHCKRRNGSVYVNHPQRFAITRYGGGYYNGGG 61

Query: 117 LRSDSRFSGVDQKVIDSLPFFRFSSLRGSKKGLECAVCLSKFEDVEVLRLLPQCKHAFHI 176
           +    + SG+D+ VI+SLP FRF +L G K GLECAVCL++FE  EVLRLLP+CKHAFH+
Sbjct: 62  VVGGRKNSGIDRSVIESLPVFRFGALSGHKDGLECAVCLARFEPTEVLRLLPKCKHAFHV 121

Query: 177 GCIDHWLENHSSCPLCRCRISVEDIAFFANSNS---MRLMRNNSQPELPQDPNMELF--V 236
            C+D WL+ HS+CPLCR R+  EDI    + NS   ++  ++ S       P +  F  V
Sbjct: 122 ECVDTWLDAHSTCPLCRYRVDPEDILLIGDCNSWFELQFSKDESNSVNNNPPGLTRFIPV 181

Query: 237 QREEDQHNSS-----------------------RFNIGSSFGKNGKGNKEKEVLIGNDGG 296
            R   +H+S+                       R ++ SS   N  G ++ E +  N   
Sbjct: 182 SRISGRHSSAGERASRLNEIRTSSSYKSNPMSFRRSLDSSLKVNDAGEEKSESVAVNCLD 241

Query: 297 ECEREEQVLF--------HKHKHKILVSEFVFVN-RWSNLSASDLMFLNTEML 302
             +R++ +L          + +H+I++S     + RWS +  SDL++L +EM+
Sbjct: 242 RLQRKDGLLLIPNRESFEGRFEHRIIISGGNRDDQRWSEVRPSDLLYLRSEMI 290

BLAST of Lag0034465 vs. TAIR 10
Match: AT4G40070.1 (RING/U-box superfamily protein )

HSP 1 Score: 105.5 bits (262), Expect = 1.1e-22
Identity = 59/164 (35.98%), Postives = 89/164 (54.27%), Query Frame = 0

Query: 33  IILIIMTTLPFDVNAQ--APPPPKPDPGIPQNLNIVIGILSIMVSMAFIFVVYVKFCHQN 92
           IIL +   +    NAQ  +P PP    G   +   V  +L  +  +  +  VY++ C   
Sbjct: 14  IILHVAIIIQSKANAQSFSPSPPDLQTGHTPSKTTVFAVLVTLFFLTGLLSVYIRHC--- 73

Query: 93  PRLRGEPRAHLGLGLLRSD---SRFSGVDQKVIDSLPFFRFSSLRGSK---KGLECAVCL 152
              R  P +       R++   SR  G+D  V++S P F +SS++ SK   K LECA+CL
Sbjct: 74  --ARSNPDSSTRYFRNRANDGSSRRGGLDNAVVESFPVFAYSSVKESKIGSKDLECAICL 133

Query: 153 SKFEDVEVLRLLPQCKHAFHIGCIDHWLENHSSCPLCRCRISVE 189
           ++ ED E +RLLP C H FHI CID WL +H++CP+CR  ++ +
Sbjct: 134 NELEDHETVRLLPICNHLFHIDCIDTWLYSHATCPVCRSNLTAK 172

BLAST of Lag0034465 vs. TAIR 10
Match: AT2G18650.1 (RING/U-box superfamily protein )

HSP 1 Score: 98.6 bits (244), Expect = 1.4e-20
Identity = 69/201 (34.33%), Postives = 99/201 (49.25%), Query Frame = 0

Query: 47  AQAPPPP-----------KPDPGIPQNLNIVIGILSIMVSMAFIFVVYVKFCHQNPRLRG 106
           + +PPPP             +  I  N+ ++I ILSI+  ++ +  + VKF     R   
Sbjct: 16  SSSPPPPLIPLKSNTSLSNLNSKITPNILLIIIILSIIFFISGLLHILVKFLLTPSRESR 75

Query: 107 EP-----RAHLGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLRGSK-KGLECAVCLSKFED 166
           E       A  G      +   SGVDQ +ID+LP F + S+ G K    +C VCL +FE 
Sbjct: 76  EDYFDNVTALQGQLQQLFNLHDSGVDQSLIDTLPVFHYKSIVGLKISPFDCPVCLCEFET 135

Query: 167 VEVLRLLPQCKHAFHIGCIDHWLENHSSCPLCRCRISVEDIAFFANSNSMRLMRNNSQPE 226
            + LRLLP+C HAFH+ CID WL +HS+CPLCR  +     +    S+S  L+  + Q  
Sbjct: 136 EDKLRLLPKCSHAFHVECIDTWLLSHSTCPLCRSNLLSGFSSHHNLSSSYLLVLESEQSS 195

Query: 227 LPQDPNMELFVQREEDQHNSS 231
               P +E   Q   D +N S
Sbjct: 196 RDMVPVLESNSQLGYDVNNDS 216