Lag0034464 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0034464
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionE3 ubiquitin-protein ligase ATL42-like
Locationchr3: 7566744 .. 7568039 (-)
RNA-Seq ExpressionLag0034464
SyntenyLag0034464
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCCGGTTAAGTTTGTTTACTGTTTGCCTCTTTTCTGTGTTCTTTTGTGTTGAAGCTCAAGTTGCTTCTCAAGATGCTGAAAACAGTGCTTTTCAGCCAAGCCTTGGCTTTGTGATAGGGATTCTGGGAGTTATGTTTTTGTTGACATTTATTCTCCTTGTCTATGCAAAGTTCTGCCATAGGAGATCTTCTATTCGAGGGGATAATGTAAACCATCTGGGACAAATCAGATCAAGCTCTCGGTTTTCCGGAATTGATAAGACGGTGATCGAGTCCCTTCCATTCTTTAGATTCTCCACCCTCAAAGGGTCCAAAGAAGGGCTGGAGTGTGCTGTCTGTCTATCGAAATTCGAAGATATCGAAATTCTCCGGTTGTTGCCAAAGTGCAAGCACGCGTTTCATATCAACTGCATCGACCATTGGCTCGAAAAACATGCTAGCTGTCCTCTTTGCAGGCAAAGAGTTTGCTCTGAGGACTTGAAGCTCCTTACTGGCTCAAGCAGCATGAGGTTCTTATGGAGCAACCTTTCAGAGCTGAAACAAGACTCAAACATAGAGCTCTTTGTACAGAGAGAAGAAGAAGAACAAGAACAACTCCATGGCTCATCTAGATTCAGCATTGGAAGAAGCTTCAGAAAGATCTTAAAGAGTGATAAAGAAGGGGAAATGTTGATACAAAAGGCCTGTGATGGAGATGAGAACATGAAGAACTTACACAGACACAATCACAAGATTATCTTATCAGATTTCGTCTTCATGAATCGATGGAGTAACGTCAGCTCCTCAGACCTGATGTTCTTGAACAAAGAGATGGTTGGTTCCATTTCAAGCAGTAGATTCACTTCCTTGGAAACAGACATTGAGCAGTCAACGCTGTCAAGATCAGTGCAAAACGAAGAAATCTTGAAGATCAAAGAGGAGATGGACATAAAGAGATCATTTGAGAGCAAACTCAGCACAATAAGCCAAAGTAACTCAGTTCCAATTCTGGAATTTCCTTCCACATCAGAATCTGCTGCAAATCCATGTCAAACAGCAAGAATTACAAGCACAGAAGCGAGAAGATCAGTTTCAGAAATCACAGGCATATCAAGATTTGGAGGTGATCTGCATATGAAGTTCAACAGAAAAGTTGAAATTGGGGAATCTTCTGATCTGGGAAACAATGTCAAGCAAGAAAGAATGAGACAAATCTGGTACCCAATTGCCAAAAGAACAGTCCAATGGTTTGCCAACAGAGAAACAAGGTTTCAACCAGCTGAGAATAGACAGCAGACAGTAGAAACTGTATAG

mRNA sequence

ATGATCCGGTTAAGTTTGTTTACTGTTTGCCTCTTTTCTGTGTTCTTTTGTGTTGAAGCTCAAGTTGCTTCTCAAGATGCTGAAAACAGTGCTTTTCAGCCAAGCCTTGGCTTTGTGATAGGGATTCTGGGAGTTATGTTTTTGTTGACATTTATTCTCCTTGTCTATGCAAAGTTCTGCCATAGGAGATCTTCTATTCGAGGGGATAATGTAAACCATCTGGGACAAATCAGATCAAGCTCTCGGTTTTCCGGAATTGATAAGACGGTGATCGAGTCCCTTCCATTCTTTAGATTCTCCACCCTCAAAGGGTCCAAAGAAGGGCTGGAGTGTGCTGTCTGTCTATCGAAATTCGAAGATATCGAAATTCTCCGGTTGTTGCCAAAGTGCAAGCACGCGTTTCATATCAACTGCATCGACCATTGGCTCGAAAAACATGCTAGCTGTCCTCTTTGCAGGCAAAGAGTTTGCTCTGAGGACTTGAAGCTCCTTACTGGCTCAAGCAGCATGAGGTTCTTATGGAGCAACCTTTCAGAGCTGAAACAAGACTCAAACATAGAGCTCTTTGTACAGAGAGAAGAAGAAGAACAAGAACAACTCCATGGCTCATCTAGATTCAGCATTGGAAGAAGCTTCAGAAAGATCTTAAAGAGTGATAAAGAAGGGGAAATGTTGATACAAAAGGCCTGTGATGGAGATGAGAACATGAAGAACTTACACAGACACAATCACAAGATTATCTTATCAGATTTCGTCTTCATGAATCGATGGAGTAACGTCAGCTCCTCAGACCTGATGTTCTTGAACAAAGAGATGGTTGGTTCCATTTCAAGCAGTAGATTCACTTCCTTGGAAACAGACATTGAGCAGTCAACGCTGTCAAGATCAGTGCAAAACGAAGAAATCTTGAAGATCAAAGAGGAGATGGACATAAAGAGATCATTTGAGAGCAAACTCAGCACAATAAGCCAAAGTAACTCAGTTCCAATTCTGGAATTTCCTTCCACATCAGAATCTGCTGCAAATCCATGTCAAACAGCAAGAATTACAAGCACAGAAGCGAGAAGATCAGTTTCAGAAATCACAGGCATATCAAGATTTGGAGGTGATCTGCATATGAAGTTCAACAGAAAAGTTGAAATTGGGGAATCTTCTGATCTGGGAAACAATGTCAAGCAAGAAAGAATGAGACAAATCTGGTACCCAATTGCCAAAAGAACAGTCCAATGGTTTGCCAACAGAGAAACAAGGTTTCAACCAGCTGAGAATAGACAGCAGACAGTAGAAACTGTATAG

Coding sequence (CDS)

ATGATCCGGTTAAGTTTGTTTACTGTTTGCCTCTTTTCTGTGTTCTTTTGTGTTGAAGCTCAAGTTGCTTCTCAAGATGCTGAAAACAGTGCTTTTCAGCCAAGCCTTGGCTTTGTGATAGGGATTCTGGGAGTTATGTTTTTGTTGACATTTATTCTCCTTGTCTATGCAAAGTTCTGCCATAGGAGATCTTCTATTCGAGGGGATAATGTAAACCATCTGGGACAAATCAGATCAAGCTCTCGGTTTTCCGGAATTGATAAGACGGTGATCGAGTCCCTTCCATTCTTTAGATTCTCCACCCTCAAAGGGTCCAAAGAAGGGCTGGAGTGTGCTGTCTGTCTATCGAAATTCGAAGATATCGAAATTCTCCGGTTGTTGCCAAAGTGCAAGCACGCGTTTCATATCAACTGCATCGACCATTGGCTCGAAAAACATGCTAGCTGTCCTCTTTGCAGGCAAAGAGTTTGCTCTGAGGACTTGAAGCTCCTTACTGGCTCAAGCAGCATGAGGTTCTTATGGAGCAACCTTTCAGAGCTGAAACAAGACTCAAACATAGAGCTCTTTGTACAGAGAGAAGAAGAAGAACAAGAACAACTCCATGGCTCATCTAGATTCAGCATTGGAAGAAGCTTCAGAAAGATCTTAAAGAGTGATAAAGAAGGGGAAATGTTGATACAAAAGGCCTGTGATGGAGATGAGAACATGAAGAACTTACACAGACACAATCACAAGATTATCTTATCAGATTTCGTCTTCATGAATCGATGGAGTAACGTCAGCTCCTCAGACCTGATGTTCTTGAACAAAGAGATGGTTGGTTCCATTTCAAGCAGTAGATTCACTTCCTTGGAAACAGACATTGAGCAGTCAACGCTGTCAAGATCAGTGCAAAACGAAGAAATCTTGAAGATCAAAGAGGAGATGGACATAAAGAGATCATTTGAGAGCAAACTCAGCACAATAAGCCAAAGTAACTCAGTTCCAATTCTGGAATTTCCTTCCACATCAGAATCTGCTGCAAATCCATGTCAAACAGCAAGAATTACAAGCACAGAAGCGAGAAGATCAGTTTCAGAAATCACAGGCATATCAAGATTTGGAGGTGATCTGCATATGAAGTTCAACAGAAAAGTTGAAATTGGGGAATCTTCTGATCTGGGAAACAATGTCAAGCAAGAAAGAATGAGACAAATCTGGTACCCAATTGCCAAAAGAACAGTCCAATGGTTTGCCAACAGAGAAACAAGGTTTCAACCAGCTGAGAATAGACAGCAGACAGTAGAAACTGTATAG

Protein sequence

MIRLSLFTVCLFSVFFCVEAQVASQDAENSAFQPSLGFVIGILGVMFLLTFILLVYAKFCHRRSSIRGDNVNHLGQIRSSSRFSGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSKFEDIEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRVCSEDLKLLTGSSSMRFLWSNLSELKQDSNIELFVQREEEEQEQLHGSSRFSIGRSFRKILKSDKEGEMLIQKACDGDENMKNLHRHNHKIILSDFVFMNRWSNVSSSDLMFLNKEMVGSISSSRFTSLETDIEQSTLSRSVQNEEILKIKEEMDIKRSFESKLSTISQSNSVPILEFPSTSESAANPCQTARITSTEARRSVSEITGISRFGGDLHMKFNRKVEIGESSDLGNNVKQERMRQIWYPIAKRTVQWFANRETRFQPAENRQQTVETV
Homology
BLAST of Lag0034464 vs. NCBI nr
Match: XP_022144841.1 (E3 ubiquitin-protein ligase ATL42-like [Momordica charantia])

HSP 1 Score: 707.2 bits (1824), Expect = 8.6e-200
Identity = 374/435 (85.98%), Postives = 393/435 (90.34%), Query Frame = 0

Query: 1   MIRLSLFTVCLFSVFFCVEAQVASQDAENSAFQPSLGFVIGILGVMFLLTFILLVYAKFC 60
           M RLSL   CLFSVFF VEAQ+  QD+ENSAFQPSLGFVIGILGVMFLLTF+LL+YAKFC
Sbjct: 1   MKRLSLVAFCLFSVFFHVEAQIDPQDSENSAFQPSLGFVIGILGVMFLLTFVLLLYAKFC 60

Query: 61  HRRSSIRGDNVNHLGQIRSSSRFSGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSKFED 120
           HRR S+RGD+VNH  QIRSSSR SGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSKFED
Sbjct: 61  HRR-SVRGDSVNHPAQIRSSSRHSGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSKFED 120

Query: 121 IEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRVCSEDLKLLTGSSSMRFLWSNLSEL 180
           IEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRV SEDLKL T SSSMR L SN SEL
Sbjct: 121 IEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRVGSEDLKLFTSSSSMRLLLSNQSEL 180

Query: 181 KQDSNIELFVQREE----EEQEQLHGSSRFSIGRSFRKILKSDKEGEMLIQKACDGDENM 240
           KQDSNIELFVQREE    E+++Q HGSSRFSIGRSFRKILKS+K+ EMLIQKACDGDENM
Sbjct: 181 KQDSNIELFVQREEEQEQEQEQQPHGSSRFSIGRSFRKILKSEKDEEMLIQKACDGDENM 240

Query: 241 KNLHRHNHKIILSDFVFMNRWSNVSSSDLMFLNKEMVGSISSSRFTSLETDIEQSTLSRS 300
           KNLHR NHKII+SDFVFMNRWSNVSSSDLMFLNKEM+GSISSSRFTS ETDIEQST  RS
Sbjct: 241 KNLHRLNHKIIVSDFVFMNRWSNVSSSDLMFLNKEMIGSISSSRFTSFETDIEQSTAPRS 300

Query: 301 VQNEEILKIKEEMDIKRSFESKLSTISQSNSVPILEFPSTSESAANPCQTARITSTEARR 360
           +QNEEILKIKEEM+IKRSF+SK STISQSNSVPIL FPSTSESAA PCQTARI S + RR
Sbjct: 301 MQNEEILKIKEEMEIKRSFDSKFSTISQSNSVPILVFPSTSESAAKPCQTARINSPDERR 360

Query: 361 SVSEITGISRFGGDLHMKFNRKVEIGESSDLGNNVKQERMRQIWYPIAKRTVQWFANRET 420
           SVSEITGISRF GDL +KFN +VE GE SDLGNN KQERMRQ WYPIAKRTVQWFANRET
Sbjct: 361 SVSEITGISRFWGDLDLKFNTRVETGEPSDLGNNGKQERMRQNWYPIAKRTVQWFANRET 420

Query: 421 RFQPAENRQQTVETV 432
           R QPAENR QTVETV
Sbjct: 421 RSQPAENRLQTVETV 434

BLAST of Lag0034464 vs. NCBI nr
Match: XP_038878637.1 (E3 ubiquitin-protein ligase ATL42-like [Benincasa hispida])

HSP 1 Score: 681.4 bits (1757), Expect = 5.0e-192
Identity = 368/439 (83.83%), Postives = 394/439 (89.75%), Query Frame = 0

Query: 1   MIRLSLFTVCLFSVFFCVEAQVASQDAENSAFQPSLGFVIGILGVMFLLTFILLVYAKFC 60
           MIRLS     LF VFF VEAQV SQDAENSAFQPSLGFVIGILGVMF+LTFILLVYAK+C
Sbjct: 1   MIRLSSIVFFLFFVFFVVEAQVDSQDAENSAFQPSLGFVIGILGVMFMLTFILLVYAKYC 60

Query: 61  HRRSSIRGDNVNHLGQIRSSSRFSGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSKFED 120
           HRRSS+RGD+V H  QIR+SSRFSGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSKFED
Sbjct: 61  HRRSSVRGDDVTHPRQIRTSSRFSGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSKFED 120

Query: 121 IEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRVCSEDLKLLTGSSSMRFLWSNLSEL 180
           IEILR+LPKCKHAFHINCIDHWLEKHASCPLCRQRV SEDLKLL+ S+SMRFL SNLSEL
Sbjct: 121 IEILRMLPKCKHAFHINCIDHWLEKHASCPLCRQRVGSEDLKLLSNSNSMRFLLSNLSEL 180

Query: 181 KQDSNIELFVQREEEEQEQ----LHGSSRFSIGRSFRKILKSDKEGEMLIQKAC-DGDEN 240
           KQDSNIELFVQREEEE++Q    LHGSSRFSIGRSFRKILKSDKE EMLIQKA  + DEN
Sbjct: 181 KQDSNIELFVQREEEEEQQQQQLLHGSSRFSIGRSFRKILKSDKEEEMLIQKAYGNEDEN 240

Query: 241 MKNLHRHNHKIILSDFVFMNRWSNVSSSDLMFLNKEMVGSISSSRFTSLETDIEQSTLSR 300
           MKNLHRHNHKII+SDFVFMNRWSNVSSSDLMFLNKEM+ +ISSSRFTS ETDIEQS+L R
Sbjct: 241 MKNLHRHNHKIIVSDFVFMNRWSNVSSSDLMFLNKEMIDAISSSRFTSFETDIEQSSLPR 300

Query: 301 SVQNEEILKIKEEMDIKRSF-ESKLSTISQSNSVPILEFPSTSESAANPCQTARITSTEA 360
           S+Q EEILK+ EEM+IKRSF +SKL+TI+QSN+  IL FPSTS+S+ NP QT RIT  +A
Sbjct: 301 SMQIEEILKVNEEMEIKRSFDQSKLNTINQSNN-SILGFPSTSQSSTNPSQTTRITGPDA 360

Query: 361 RRSVSEITGISRFGGD--LHMKFNRKVEIGESSDLGNNVKQERMRQIWYPIAKRTVQWFA 420
           RRSVSEITG SRFG D  L+  FNRK E GESSDLGNNVKQER RQIWYPIAKRTVQWFA
Sbjct: 361 RRSVSEITGRSRFGDDVGLYTNFNRKDESGESSDLGNNVKQERTRQIWYPIAKRTVQWFA 420

Query: 421 NRETRFQPAENRQQTVETV 432
           NRETRFQPAENRQQTVETV
Sbjct: 421 NRETRFQPAENRQQTVETV 438

BLAST of Lag0034464 vs. NCBI nr
Match: XP_008462695.1 (PREDICTED: E3 ubiquitin-protein ligase ATL42-like [Cucumis melo] >KAA0046521.1 E3 ubiquitin-protein ligase ATL42-like [Cucumis melo var. makuwa] >TYK28738.1 E3 ubiquitin-protein ligase ATL42-like [Cucumis melo var. makuwa])

HSP 1 Score: 674.1 bits (1738), Expect = 8.1e-190
Identity = 367/438 (83.79%), Postives = 392/438 (89.50%), Query Frame = 0

Query: 1   MIRLSLFTVCLFSVFFCVEAQVASQDAENSAFQPSLGFVIGILGVMFLLTFILLVYAKFC 60
           MI+LSL    LFSVFF VEAQ+ SQDAENSAFQPSLGFVIGILGVMFLLTFILLVYAKFC
Sbjct: 1   MIQLSLNVCFLFSVFFVVEAQIDSQDAENSAFQPSLGFVIGILGVMFLLTFILLVYAKFC 60

Query: 61  HRRSSIRGDNVNHLGQIRSSSRFSGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSKFED 120
           HRR+SI  D VNH  QIRSSSRFSGIDKTVIESLPFFRFSTLKG+KEGLECAVCLSKFED
Sbjct: 61  HRRASISVDGVNHPRQIRSSSRFSGIDKTVIESLPFFRFSTLKGTKEGLECAVCLSKFED 120

Query: 121 IEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRVCSEDLKLLTGSSSMRFLWSNLSEL 180
           IEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRV SEDLKLL+ SSSMRFL SNLSEL
Sbjct: 121 IEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRVGSEDLKLLSNSSSMRFLLSNLSEL 180

Query: 181 KQDSNIELFVQREEEEQEQ---LHGSSRFSIGRSFRKILKSDKEGEMLI-QKACD-GDEN 240
           KQDSNIELFVQREEEEQ+Q   LHGSSRFSI RSFRKILK+DKE EMLI Q +CD  DE 
Sbjct: 181 KQDSNIELFVQREEEEQQQQQILHGSSRFSIRRSFRKILKNDKENEMLIPQASCDYEDEK 240

Query: 241 MKNLHRHNHKIILSDFVFMNRWSNVSSSDLMFLNKEMVGSISSSRFTSLETDIEQSTLSR 300
           MKNLHRHNHKII+SDFVFMNRWSN+SSSDLMFLNKEM+ +ISS RFTSL+TDIEQSTL R
Sbjct: 241 MKNLHRHNHKIIVSDFVFMNRWSNISSSDLMFLNKEMIDAISSCRFTSLDTDIEQSTLPR 300

Query: 301 SVQNEEILKIKEEMDIKRSFESKLSTISQSNSVPILEFPSTSESAANPCQTARITSTEAR 360
           S+Q +EILKIKEEM+IKRSFESKL+T  QSNS  IL +PSTS+S+ NP QT RITS +AR
Sbjct: 301 SMQIDEILKIKEEMEIKRSFESKLNTKIQSNS--ILGYPSTSQSSTNPSQTTRITSPDAR 360

Query: 361 RSVSEITGISRFG--GDLHMKFNRKVEIGESSDLGNNVKQERMRQIWYPIAKRTVQWFAN 420
           RSVSEITGISRFG   DL+M FNRKVE GESSDL ++VKQERMR+IWYPIAKRTVQWFAN
Sbjct: 361 RSVSEITGISRFGHDDDLYMNFNRKVENGESSDLESSVKQERMRKIWYPIAKRTVQWFAN 420

Query: 421 RETRFQPAENRQQTVETV 432
           RE RFQ AENRQQ VETV
Sbjct: 421 REKRFQTAENRQQIVETV 436

BLAST of Lag0034464 vs. NCBI nr
Match: KAG6587983.1 (E3 ubiquitin-protein ligase ATL42, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 586.6 bits (1511), Expect = 1.7e-163
Identity = 318/441 (72.11%), Postives = 356/441 (80.73%), Query Frame = 0

Query: 1   MIRLSLFTVCLFSVFFCVEAQVASQDAENSAFQPSLGFVIGILGVMFLLTFILLVYAKFC 60
           MIRL+L T CLFSV F V+AQ +SQDAEN +FQP+LGFVIGILGVMF+LTF+LLVYAK+C
Sbjct: 183 MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 242

Query: 61  HRRSSIRGDN-VNHLGQIRSSSR--FSGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSK 120
           H RSS+  +N V H GQIRSSS     GIDKTVIESLPFFRFSTLKGSK+GLECA+CLSK
Sbjct: 243 HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 302

Query: 121 FEDIEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRVCSEDLKLLTGSSSMRFLWSNL 180
           FEDIEILRLLPKCKHAFHINCIDHWLE+HA+CPLCRQ V SEDL+L T SSSMRFLWSNL
Sbjct: 303 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 362

Query: 181 SELKQDSNIELFVQREEEE-------QEQLHGSSRFSIGRSFRKILKSDKEGEMLIQKAC 240
           SELKQDSNIELFVQREEEE       Q+Q+HGS RFSIGRSFRK+LKSDKEGEMLIQK  
Sbjct: 363 SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY 422

Query: 241 DGDENMKNLHRHNHKIILSDFVFMNRWSNVSSSDLMFLNKEMVGSISSSRFTSLETDIEQ 300
           DGD+NMKNLHRHNHKII+SDFVFMNRWSNVSSSD+MFLNKEM+ SISS RFTSLET +E+
Sbjct: 423 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE 482

Query: 301 STLSRSVQNEEILKIKEEMDIKRSFESKLSTISQSNSVPILEFPSTSESAANPCQTARIT 360
           STLSRSV+NEE                           P+  FPSTS+       +ARIT
Sbjct: 483 STLSRSVENEE---------------------------PLKGFPSTSDC------SARIT 542

Query: 361 STEARRSVSEITGISRFGGDLHMKFNRKVEIGESSDLGNNVKQERMRQIWYPIAKRTVQW 420
           STEARRSVSEITG++RFGGD+HMKFNR++E+GESS+L NNVK    RQ WYPIA++TVQW
Sbjct: 543 STEARRSVSEITGVTRFGGDVHMKFNRRIEMGESSELENNVK----RQTWYPIARKTVQW 580

Query: 421 FANRETRFQPAENRQQTVETV 432
           FANRE      + RQ  VETV
Sbjct: 603 FANRE------KTRQHRVETV 580

BLAST of Lag0034464 vs. NCBI nr
Match: XP_022929946.1 (E3 ubiquitin-protein ligase ATL42-like [Cucurbita moschata])

HSP 1 Score: 586.6 bits (1511), Expect = 1.7e-163
Identity = 318/441 (72.11%), Postives = 356/441 (80.73%), Query Frame = 0

Query: 1   MIRLSLFTVCLFSVFFCVEAQVASQDAENSAFQPSLGFVIGILGVMFLLTFILLVYAKFC 60
           MIRL+L T CLFSV F V+AQ +SQDAEN +FQP+LGFVIGILGVMF+LTF+LLVYAK+C
Sbjct: 40  MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 99

Query: 61  HRRSSIRGDN-VNHLGQIRSSSR--FSGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSK 120
           H RSS+  +N V H GQIRSSS     GIDKTVIESLPFFRFSTLKGSK+GLECA+CLSK
Sbjct: 100 HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 159

Query: 121 FEDIEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRVCSEDLKLLTGSSSMRFLWSNL 180
           FEDIEILRLLPKCKHAFHINCIDHWLE+HA+CPLCRQ V SEDL+L T SSSMRFLWSNL
Sbjct: 160 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 219

Query: 181 SELKQDSNIELFVQREEEE-------QEQLHGSSRFSIGRSFRKILKSDKEGEMLIQKAC 240
           SELKQDSNIELFVQREEEE       Q+Q+HGS RFSIGRSFRK+LKSDKEGEMLIQK  
Sbjct: 220 SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY 279

Query: 241 DGDENMKNLHRHNHKIILSDFVFMNRWSNVSSSDLMFLNKEMVGSISSSRFTSLETDIEQ 300
           DGD+NMKNLHRHNHKII+SDFVFMNRWSNVSSSD+MFLNKEM+ SISS RFTSLET +E+
Sbjct: 280 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE 339

Query: 301 STLSRSVQNEEILKIKEEMDIKRSFESKLSTISQSNSVPILEFPSTSESAANPCQTARIT 360
           STLSRSV+NEE                           P+  FPSTS+       +ARIT
Sbjct: 340 STLSRSVENEE---------------------------PLRGFPSTSDC------SARIT 399

Query: 361 STEARRSVSEITGISRFGGDLHMKFNRKVEIGESSDLGNNVKQERMRQIWYPIAKRTVQW 420
           STEARRSVSEITG++RFGGD+HMKFNR++E+GESS+L NNVK    RQ WYPIA++TVQW
Sbjct: 400 STEARRSVSEITGVTRFGGDVHMKFNRRIEMGESSELENNVK----RQTWYPIARKTVQW 437

Query: 421 FANRETRFQPAENRQQTVETV 432
           FANRE      + RQ  VETV
Sbjct: 460 FANRE------KTRQHRVETV 437

BLAST of Lag0034464 vs. ExPASy Swiss-Prot
Match: Q5XF85 (E3 ubiquitin-protein ligase ATL42 OS=Arabidopsis thaliana OX=3702 GN=ATL42 PE=1 SV=2)

HSP 1 Score: 355.1 bits (910), Expect = 1.1e-96
Identity = 205/407 (50.37%), Postives = 270/407 (66.34%), Query Frame = 0

Query: 32  FQPSLGFVIGILGVMFLLTFILLVYAKFCH-RRSSIRGDNVNHLGQIR----------SS 91
           F+PSL  V G+L +MF LTF+LLVYAK CH    S  GD   H  ++R          SS
Sbjct: 33  FEPSLAVVTGVLAIMFALTFVLLVYAKCCHIDLRSGSGDRRRHDRRLRQGIFFNRSTASS 92

Query: 92  SRFSGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSKFEDIEILRLLPKCKHAFHINCID 151
            RFSG+DKT IESLP FRFS LKGSK+GL+C+VCLSKFE +EILRLLPKC+HAFHI CID
Sbjct: 93  DRFSGLDKTAIESLPLFRFSALKGSKQGLDCSVCLSKFESVEILRLLPKCRHAFHIGCID 152

Query: 152 HWLEKHASCPLCRQRV-CSEDLKLLTGSSSMRFLWSNLSELKQDSNIELFVQREEEEQ-- 211
            WLE+HA+CPLCR RV   ED  +LT  +S RFL  N SE+++DS++EL+++REEEE+  
Sbjct: 153 QWLEQHATCPLCRDRVSMEEDSSVLTNGNSFRFL--NQSEIREDSSLELYIEREEEEERI 212

Query: 212 --EQLHGSSRFSIGRSFRKILKSDKEGEMLIQKACDGDENMKNLHRHNHKIILSDFVFMN 271
             E+L GSSRFSIG SFRKILK   + + L+ +  +  +  K +H+ NH+I++SD VF N
Sbjct: 213 HREELSGSSRFSIGESFRKILKLGNKEKTLLDEHVNDKDEKKLMHKFNHRIVVSDVVFKN 272

Query: 272 RWSNVSSSDLMFLNKEMVGSISSSRFTSLETDIEQSTLSRSVQNEE--ILKIKEEMDIKR 331
           RWSNVSSSDLMFLN EMV SISS RF+SL+       + R  + ++  IL+IKEEM+ KR
Sbjct: 273 RWSNVSSSDLMFLNSEMVNSISSERFSSLD------HVKRGDEEDQIGILRIKEEMEAKR 332

Query: 332 SFESKLSTISQSNSVPILEFPSTSESAANPCQTARITSTEARRSVSEITGISRFGGDLHM 391
             E+KL++++   S         SE+  +  ++  +     RRSVS+IT + R    +H 
Sbjct: 333 MLENKLTSMTTMFS---------SENGDSGSKSRSVMIEPGRRSVSDITAVPRLSISIHG 392

Query: 392 KFN-RKVEIGESSDLGNNVKQERMRQIWYPIAKRTVQWFANRETRFQ 420
             +    E   +   G N  +ER R++W PIA++T QWFANRE R Q
Sbjct: 393 DCSGSAAETASALQNGGNETEERRRRLWLPIARKTAQWFANREKRSQ 422

BLAST of Lag0034464 vs. ExPASy Swiss-Prot
Match: Q9SL78 (Putative RING-H2 finger protein ATL12 OS=Arabidopsis thaliana OX=3702 GN=ATL12 PE=3 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 4.4e-74
Identity = 178/409 (43.52%), Postives = 239/409 (58.44%), Query Frame = 0

Query: 27  AENSAFQPSLGFVIGILGVMFLLTFILLVYAKFCHR----RSSIRGDNVNH----LGQIR 86
           A +  F+PSL  + G+  ++F LTF+LLVYAK  H      +   G+ + H     G   
Sbjct: 32  ATSDLFKPSLAIITGVFSIVFTLTFVLLVYAKCFHNDLRSETDSDGERIRHDRLWQGLFN 91

Query: 87  SSSRFSGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSKFEDIEILRLLPKCKHAFHINC 146
            SSRFSG+DK  IESLPFFRFS LKG K+GLEC+VCLSKFED+EILRLLPKC+HAFHI C
Sbjct: 92  RSSRFSGLDKKAIESLPFFRFSALKGLKQGLECSVCLSKFEDVEILRLLPKCRHAFHIGC 151

Query: 147 IDHWLEKHASCPLCRQRVCSEDLKLLTGSSSMRFLWSNLSELK-QDSNIELFVQREEEEQ 206
           ID WLE+HA+CPLCR RV  ED   + G+SS      N SE + +DS +E++++REE   
Sbjct: 152 IDQWLEQHATCPLCRNRVNIEDDLSVLGNSSTSLRILNQSETREEDSRLEIYIEREEGTN 211

Query: 207 EQLHGSSRFSIGRSFRKILKSDKEGEMLIQKACDGDENM---KNLHRHNHKIILSDFVFM 266
           +   GSSRFS   SFRKILK      +L+++  +G+EN+   K +H+ NH+I++SD VF 
Sbjct: 212 D---GSSRFS---SFRKILKK----SLLLER--EGNENIDEKKLMHKFNHRIVVSDAVFK 271

Query: 267 NRWSNVSSSDLMFLNKEMVGSISSSRFTSLETDIEQSTLSRSVQNEEILKIKEEMDIKRS 326
           NRWSN++SSDL FL  EM+ S+SS RF+S++             +   L+ KE+M++KR 
Sbjct: 272 NRWSNITSSDLTFLTSEMLNSVSSDRFSSVDR-----------VHRGNLRDKEDMEMKRM 331

Query: 327 FESKLSTISQSNSVPILEFPSTSESAANPCQTARITSTEARRSVSEITGISR---FGGDL 386
                  I   +S                          +RR+VSEIT +SR    GG  
Sbjct: 332 L------IKHKDS--------------------------SRRTVSEITTVSREKAVGGSY 376

Query: 387 HMKFNRKVEIGESSDLGNN-----VKQERMRQIWYPIAKRTVQWFANRE 416
                     G ++    N       +ER R++W PIA+RT QWF NRE
Sbjct: 392 R---------GSTASTSQNYAVTATTEERRRRLWLPIARRTAQWFVNRE 376

BLAST of Lag0034464 vs. ExPASy Swiss-Prot
Match: Q5EAE9 (RING-H2 finger protein ATL43 OS=Arabidopsis thaliana OX=3702 GN=ATL43 PE=2 SV=2)

HSP 1 Score: 154.8 bits (390), Expect = 2.2e-36
Identity = 107/297 (36.03%), Postives = 147/297 (49.49%), Query Frame = 0

Query: 30  SAFQPSLGFVIGILGVMFLLTFILLVYAKFCHRRS-------------SIRGDNVNHLGQ 89
           S+  P +  VI +L   F LTF+LL+Y K C RR+             +  G    + G 
Sbjct: 52  SSLMPGIAVVIAVLTAFFSLTFLLLLYVKHCKRRNGSVYVNHPQRFAITRYGGGYYNGGG 111

Query: 90  IRSSSRFSGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSKFEDIEILRLLPKCKHAFHI 149
           +    + SGID++VIESLP FRF  L G K+GLECAVCL++FE  E+LRLLPKCKHAFH+
Sbjct: 112 VVGGRKNSGIDRSVIESLPVFRFGALSGHKDGLECAVCLARFEPTEVLRLLPKCKHAFHV 171

Query: 150 NCIDHWLEKHASCPLCRQRVCSEDLKLLTGSSSMRFLWSNLSELKQDSN--------IEL 209
            C+D WL+ H++CPLCR RV  ED+ L+   +S    W  L   K +SN        +  
Sbjct: 172 ECVDTWLDAHSTCPLCRYRVDPEDILLIGDCNS----WFELQFSKDESNSVNNNPPGLTR 231

Query: 210 FV-------------QREEEEQEQLHGSSRFSIGRSFRKILKS--------DKEGEMLIQ 269
           F+             +R     E    SS  S   SFR+ L S        +++ E +  
Sbjct: 232 FIPVSRISGRHSSAGERASRLNEIRTSSSYKSNPMSFRRSLDSSLKVNDAGEEKSESVAV 291

Query: 270 KACDGDENMKNL----------HRHNHKIILSDFVFMN-RWSNVSSSDLMFLNKEMV 274
              D  +    L           R  H+II+S     + RWS V  SDL++L  EM+
Sbjct: 292 NCLDRLQRKDGLLLIPNRESFEGRFEHRIIISGGNRDDQRWSEVRPSDLLYLRSEMI 344

BLAST of Lag0034464 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.2e-23
Identity = 56/136 (41.18%), Postives = 87/136 (63.97%), Query Frame = 0

Query: 27  AENSAFQPSLGFVIGILGVMFLLTFILLVYAKFCHRR---SSIRGDNVNHLGQ------I 86
           + +S+F P L  +IGIL    +L     + +K+CHR    SS    N+NH G+       
Sbjct: 49  SSSSSFSPLLIALIGILTSALILVSYYTLISKYCHRHHQTSSSETLNLNHNGEGFFSSTQ 108

Query: 87  RSSSRFSGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSKFEDIEILRLLPKCKHAFHIN 146
           R S+   G+++++I+S+  +++ +  G  +G +C+VCLS+FE+ E LRLLPKC HAFH+ 
Sbjct: 109 RISTNGDGLNESMIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLP 168

Query: 147 CIDHWLEKHASCPLCR 154
           CID WL+ H++CPLCR
Sbjct: 169 CIDTWLKSHSNCPLCR 184

BLAST of Lag0034464 vs. ExPASy Swiss-Prot
Match: Q8W571 (RING-H2 finger protein ATL32 OS=Arabidopsis thaliana OX=3702 GN=ATL32 PE=2 SV=3)

HSP 1 Score: 110.9 bits (276), Expect = 3.6e-23
Identity = 57/132 (43.18%), Postives = 77/132 (58.33%), Query Frame = 0

Query: 34  PSLGFVIGILGVMFLLTFILLVYAKFCHRRSSIRGDNVNHLGQIRSSSRFSGIDKTVIES 93
           PS   V  +L  +F LT +L VY + C R +               SSR  G+D  V+ES
Sbjct: 44  PSKTTVFAVLVTLFFLTGLLSVYIRHCARSNPDSSTRYFRNRANDGSSRRGGLDNAVVES 103

Query: 94  LPFFRFSTLKGSKEG---LECAVCLSKFEDIEILRLLPKCKHAFHINCIDHWLEKHASCP 153
            P F +S++K SK G   LECA+CL++ ED E +RLLP C H FHI+CID WL  HA+CP
Sbjct: 104 FPVFAYSSVKESKIGSKDLECAICLNELEDHETVRLLPICNHLFHIDCIDTWLYSHATCP 163

Query: 154 LCRQRVCSEDLK 163
           +CR  + ++  K
Sbjct: 164 VCRSNLTAKSNK 175

BLAST of Lag0034464 vs. ExPASy TrEMBL
Match: A0A6J1CUL6 (E3 ubiquitin-protein ligase ATL42-like OS=Momordica charantia OX=3673 GN=LOC111014427 PE=4 SV=1)

HSP 1 Score: 707.2 bits (1824), Expect = 4.2e-200
Identity = 374/435 (85.98%), Postives = 393/435 (90.34%), Query Frame = 0

Query: 1   MIRLSLFTVCLFSVFFCVEAQVASQDAENSAFQPSLGFVIGILGVMFLLTFILLVYAKFC 60
           M RLSL   CLFSVFF VEAQ+  QD+ENSAFQPSLGFVIGILGVMFLLTF+LL+YAKFC
Sbjct: 1   MKRLSLVAFCLFSVFFHVEAQIDPQDSENSAFQPSLGFVIGILGVMFLLTFVLLLYAKFC 60

Query: 61  HRRSSIRGDNVNHLGQIRSSSRFSGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSKFED 120
           HRR S+RGD+VNH  QIRSSSR SGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSKFED
Sbjct: 61  HRR-SVRGDSVNHPAQIRSSSRHSGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSKFED 120

Query: 121 IEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRVCSEDLKLLTGSSSMRFLWSNLSEL 180
           IEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRV SEDLKL T SSSMR L SN SEL
Sbjct: 121 IEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRVGSEDLKLFTSSSSMRLLLSNQSEL 180

Query: 181 KQDSNIELFVQREE----EEQEQLHGSSRFSIGRSFRKILKSDKEGEMLIQKACDGDENM 240
           KQDSNIELFVQREE    E+++Q HGSSRFSIGRSFRKILKS+K+ EMLIQKACDGDENM
Sbjct: 181 KQDSNIELFVQREEEQEQEQEQQPHGSSRFSIGRSFRKILKSEKDEEMLIQKACDGDENM 240

Query: 241 KNLHRHNHKIILSDFVFMNRWSNVSSSDLMFLNKEMVGSISSSRFTSLETDIEQSTLSRS 300
           KNLHR NHKII+SDFVFMNRWSNVSSSDLMFLNKEM+GSISSSRFTS ETDIEQST  RS
Sbjct: 241 KNLHRLNHKIIVSDFVFMNRWSNVSSSDLMFLNKEMIGSISSSRFTSFETDIEQSTAPRS 300

Query: 301 VQNEEILKIKEEMDIKRSFESKLSTISQSNSVPILEFPSTSESAANPCQTARITSTEARR 360
           +QNEEILKIKEEM+IKRSF+SK STISQSNSVPIL FPSTSESAA PCQTARI S + RR
Sbjct: 301 MQNEEILKIKEEMEIKRSFDSKFSTISQSNSVPILVFPSTSESAAKPCQTARINSPDERR 360

Query: 361 SVSEITGISRFGGDLHMKFNRKVEIGESSDLGNNVKQERMRQIWYPIAKRTVQWFANRET 420
           SVSEITGISRF GDL +KFN +VE GE SDLGNN KQERMRQ WYPIAKRTVQWFANRET
Sbjct: 361 SVSEITGISRFWGDLDLKFNTRVETGEPSDLGNNGKQERMRQNWYPIAKRTVQWFANRET 420

Query: 421 RFQPAENRQQTVETV 432
           R QPAENR QTVETV
Sbjct: 421 RSQPAENRLQTVETV 434

BLAST of Lag0034464 vs. ExPASy TrEMBL
Match: A0A5D3DZ48 (E3 ubiquitin-protein ligase ATL42-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00770 PE=4 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 3.9e-190
Identity = 367/438 (83.79%), Postives = 392/438 (89.50%), Query Frame = 0

Query: 1   MIRLSLFTVCLFSVFFCVEAQVASQDAENSAFQPSLGFVIGILGVMFLLTFILLVYAKFC 60
           MI+LSL    LFSVFF VEAQ+ SQDAENSAFQPSLGFVIGILGVMFLLTFILLVYAKFC
Sbjct: 1   MIQLSLNVCFLFSVFFVVEAQIDSQDAENSAFQPSLGFVIGILGVMFLLTFILLVYAKFC 60

Query: 61  HRRSSIRGDNVNHLGQIRSSSRFSGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSKFED 120
           HRR+SI  D VNH  QIRSSSRFSGIDKTVIESLPFFRFSTLKG+KEGLECAVCLSKFED
Sbjct: 61  HRRASISVDGVNHPRQIRSSSRFSGIDKTVIESLPFFRFSTLKGTKEGLECAVCLSKFED 120

Query: 121 IEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRVCSEDLKLLTGSSSMRFLWSNLSEL 180
           IEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRV SEDLKLL+ SSSMRFL SNLSEL
Sbjct: 121 IEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRVGSEDLKLLSNSSSMRFLLSNLSEL 180

Query: 181 KQDSNIELFVQREEEEQEQ---LHGSSRFSIGRSFRKILKSDKEGEMLI-QKACD-GDEN 240
           KQDSNIELFVQREEEEQ+Q   LHGSSRFSI RSFRKILK+DKE EMLI Q +CD  DE 
Sbjct: 181 KQDSNIELFVQREEEEQQQQQILHGSSRFSIRRSFRKILKNDKENEMLIPQASCDYEDEK 240

Query: 241 MKNLHRHNHKIILSDFVFMNRWSNVSSSDLMFLNKEMVGSISSSRFTSLETDIEQSTLSR 300
           MKNLHRHNHKII+SDFVFMNRWSN+SSSDLMFLNKEM+ +ISS RFTSL+TDIEQSTL R
Sbjct: 241 MKNLHRHNHKIIVSDFVFMNRWSNISSSDLMFLNKEMIDAISSCRFTSLDTDIEQSTLPR 300

Query: 301 SVQNEEILKIKEEMDIKRSFESKLSTISQSNSVPILEFPSTSESAANPCQTARITSTEAR 360
           S+Q +EILKIKEEM+IKRSFESKL+T  QSNS  IL +PSTS+S+ NP QT RITS +AR
Sbjct: 301 SMQIDEILKIKEEMEIKRSFESKLNTKIQSNS--ILGYPSTSQSSTNPSQTTRITSPDAR 360

Query: 361 RSVSEITGISRFG--GDLHMKFNRKVEIGESSDLGNNVKQERMRQIWYPIAKRTVQWFAN 420
           RSVSEITGISRFG   DL+M FNRKVE GESSDL ++VKQERMR+IWYPIAKRTVQWFAN
Sbjct: 361 RSVSEITGISRFGHDDDLYMNFNRKVENGESSDLESSVKQERMRKIWYPIAKRTVQWFAN 420

Query: 421 RETRFQPAENRQQTVETV 432
           RE RFQ AENRQQ VETV
Sbjct: 421 REKRFQTAENRQQIVETV 436

BLAST of Lag0034464 vs. ExPASy TrEMBL
Match: A0A1S3CHK3 (E3 ubiquitin-protein ligase ATL42-like OS=Cucumis melo OX=3656 GN=LOC103500995 PE=4 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 3.9e-190
Identity = 367/438 (83.79%), Postives = 392/438 (89.50%), Query Frame = 0

Query: 1   MIRLSLFTVCLFSVFFCVEAQVASQDAENSAFQPSLGFVIGILGVMFLLTFILLVYAKFC 60
           MI+LSL    LFSVFF VEAQ+ SQDAENSAFQPSLGFVIGILGVMFLLTFILLVYAKFC
Sbjct: 1   MIQLSLNVCFLFSVFFVVEAQIDSQDAENSAFQPSLGFVIGILGVMFLLTFILLVYAKFC 60

Query: 61  HRRSSIRGDNVNHLGQIRSSSRFSGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSKFED 120
           HRR+SI  D VNH  QIRSSSRFSGIDKTVIESLPFFRFSTLKG+KEGLECAVCLSKFED
Sbjct: 61  HRRASISVDGVNHPRQIRSSSRFSGIDKTVIESLPFFRFSTLKGTKEGLECAVCLSKFED 120

Query: 121 IEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRVCSEDLKLLTGSSSMRFLWSNLSEL 180
           IEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRV SEDLKLL+ SSSMRFL SNLSEL
Sbjct: 121 IEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRVGSEDLKLLSNSSSMRFLLSNLSEL 180

Query: 181 KQDSNIELFVQREEEEQEQ---LHGSSRFSIGRSFRKILKSDKEGEMLI-QKACD-GDEN 240
           KQDSNIELFVQREEEEQ+Q   LHGSSRFSI RSFRKILK+DKE EMLI Q +CD  DE 
Sbjct: 181 KQDSNIELFVQREEEEQQQQQILHGSSRFSIRRSFRKILKNDKENEMLIPQASCDYEDEK 240

Query: 241 MKNLHRHNHKIILSDFVFMNRWSNVSSSDLMFLNKEMVGSISSSRFTSLETDIEQSTLSR 300
           MKNLHRHNHKII+SDFVFMNRWSN+SSSDLMFLNKEM+ +ISS RFTSL+TDIEQSTL R
Sbjct: 241 MKNLHRHNHKIIVSDFVFMNRWSNISSSDLMFLNKEMIDAISSCRFTSLDTDIEQSTLPR 300

Query: 301 SVQNEEILKIKEEMDIKRSFESKLSTISQSNSVPILEFPSTSESAANPCQTARITSTEAR 360
           S+Q +EILKIKEEM+IKRSFESKL+T  QSNS  IL +PSTS+S+ NP QT RITS +AR
Sbjct: 301 SMQIDEILKIKEEMEIKRSFESKLNTKIQSNS--ILGYPSTSQSSTNPSQTTRITSPDAR 360

Query: 361 RSVSEITGISRFG--GDLHMKFNRKVEIGESSDLGNNVKQERMRQIWYPIAKRTVQWFAN 420
           RSVSEITGISRFG   DL+M FNRKVE GESSDL ++VKQERMR+IWYPIAKRTVQWFAN
Sbjct: 361 RSVSEITGISRFGHDDDLYMNFNRKVENGESSDLESSVKQERMRKIWYPIAKRTVQWFAN 420

Query: 421 RETRFQPAENRQQTVETV 432
           RE RFQ AENRQQ VETV
Sbjct: 421 REKRFQTAENRQQIVETV 436

BLAST of Lag0034464 vs. ExPASy TrEMBL
Match: A0A6J1EPJ8 (E3 ubiquitin-protein ligase ATL42-like OS=Cucurbita moschata OX=3662 GN=LOC111436403 PE=4 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 8.2e-164
Identity = 318/441 (72.11%), Postives = 356/441 (80.73%), Query Frame = 0

Query: 1   MIRLSLFTVCLFSVFFCVEAQVASQDAENSAFQPSLGFVIGILGVMFLLTFILLVYAKFC 60
           MIRL+L T CLFSV F V+AQ +SQDAEN +FQP+LGFVIGILGVMF+LTF+LLVYAK+C
Sbjct: 40  MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 99

Query: 61  HRRSSIRGDN-VNHLGQIRSSSR--FSGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSK 120
           H RSS+  +N V H GQIRSSS     GIDKTVIESLPFFRFSTLKGSK+GLECA+CLSK
Sbjct: 100 HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 159

Query: 121 FEDIEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRVCSEDLKLLTGSSSMRFLWSNL 180
           FEDIEILRLLPKCKHAFHINCIDHWLE+HA+CPLCRQ V SEDL+L T SSSMRFLWSNL
Sbjct: 160 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 219

Query: 181 SELKQDSNIELFVQREEEE-------QEQLHGSSRFSIGRSFRKILKSDKEGEMLIQKAC 240
           SELKQDSNIELFVQREEEE       Q+Q+HGS RFSIGRSFRK+LKSDKEGEMLIQK  
Sbjct: 220 SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY 279

Query: 241 DGDENMKNLHRHNHKIILSDFVFMNRWSNVSSSDLMFLNKEMVGSISSSRFTSLETDIEQ 300
           DGD+NMKNLHRHNHKII+SDFVFMNRWSNVSSSD+MFLNKEM+ SISS RFTSLET +E+
Sbjct: 280 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE 339

Query: 301 STLSRSVQNEEILKIKEEMDIKRSFESKLSTISQSNSVPILEFPSTSESAANPCQTARIT 360
           STLSRSV+NEE                           P+  FPSTS+       +ARIT
Sbjct: 340 STLSRSVENEE---------------------------PLRGFPSTSDC------SARIT 399

Query: 361 STEARRSVSEITGISRFGGDLHMKFNRKVEIGESSDLGNNVKQERMRQIWYPIAKRTVQW 420
           STEARRSVSEITG++RFGGD+HMKFNR++E+GESS+L NNVK    RQ WYPIA++TVQW
Sbjct: 400 STEARRSVSEITGVTRFGGDVHMKFNRRIEMGESSELENNVK----RQTWYPIARKTVQW 437

Query: 421 FANRETRFQPAENRQQTVETV 432
           FANRE      + RQ  VETV
Sbjct: 460 FANRE------KTRQHRVETV 437

BLAST of Lag0034464 vs. ExPASy TrEMBL
Match: A0A6J1KW64 (E3 ubiquitin-protein ligase ATL42-like OS=Cucurbita maxima OX=3661 GN=LOC111499247 PE=4 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 1.4e-163
Identity = 318/439 (72.44%), Postives = 354/439 (80.64%), Query Frame = 0

Query: 1   MIRLSLFTVCLFSVFFCVEAQVASQDAENSAFQPSLGFVIGILGVMFLLTFILLVYAKFC 60
           MIRL+L T CLFSV F V+AQ +SQDAEN +FQP+LGFVIGILGVMF+LTF+LLVYAK+C
Sbjct: 1   MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60

Query: 61  HRRSSIRGDN-VNHLGQIRSSSR--FSGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSK 120
           H RSS+  +N V H GQIRSSS     GIDKTVIESLPFFRFSTLKGSK+GLECA+CLSK
Sbjct: 61  HNRSSVHVNNGVTHGGQIRSSSSTPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 120

Query: 121 FEDIEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRVCSEDLKLLTGSSSMRFLWSNL 180
           FEDIEILRLLPKCKHAFHINCIDHWLE+HA+CPLCRQ V SEDL+L T SSSMRFLWSNL
Sbjct: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180

Query: 181 SELKQDSNIELFVQREEEE-----QEQLHGSSRFSIGRSFRKILKSDKEGEMLIQKACDG 240
           SELKQDSNIELFVQREEEE     Q+Q+HGS RFSIGRSFRK+LK DKEGEMLIQK  DG
Sbjct: 181 SELKQDSNIELFVQREEEEEEEHQQQQIHGSFRFSIGRSFRKMLKGDKEGEMLIQKDYDG 240

Query: 241 DENMKNLHRHNHKIILSDFVFMNRWSNVSSSDLMFLNKEMVGSISSSRFTSLETDIEQST 300
           D+NMKNLHRHNHKII+SDFVFMNRWSNVSSSD+MFLNKEM+ SISS RFT LET +E+ST
Sbjct: 241 DKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTFLETYLEEST 300

Query: 301 LSRSVQNEEILKIKEEMDIKRSFESKLSTISQSNSVPILEFPSTSESAANPCQTARITST 360
           LSRSV+NEE                           P+  FPSTS+       +ARITST
Sbjct: 301 LSRSVENEE---------------------------PLKGFPSTSDC------SARITST 360

Query: 361 EARRSVSEITGISRFGGDLHMKFNRKVEIGESSDLGNNVKQERMRQIWYPIAKRTVQWFA 420
           EARRSVSEITG+SRFGGD+HMKFNRK+E+GESS+L NNVK    RQ WYPIA++TVQWFA
Sbjct: 361 EARRSVSEITGVSRFGGDVHMKFNRKIEMGESSELENNVK----RQTWYPIARKTVQWFA 396

Query: 421 NRETRFQPAENRQQTVETV 432
           NRE      + RQ  VETV
Sbjct: 421 NRE------KTRQHRVETV 396

BLAST of Lag0034464 vs. TAIR 10
Match: AT4G28890.1 (RING/U-box superfamily protein )

HSP 1 Score: 355.1 bits (910), Expect = 7.7e-98
Identity = 205/407 (50.37%), Postives = 270/407 (66.34%), Query Frame = 0

Query: 32  FQPSLGFVIGILGVMFLLTFILLVYAKFCH-RRSSIRGDNVNHLGQIR----------SS 91
           F+PSL  V G+L +MF LTF+LLVYAK CH    S  GD   H  ++R          SS
Sbjct: 33  FEPSLAVVTGVLAIMFALTFVLLVYAKCCHIDLRSGSGDRRRHDRRLRQGIFFNRSTASS 92

Query: 92  SRFSGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSKFEDIEILRLLPKCKHAFHINCID 151
            RFSG+DKT IESLP FRFS LKGSK+GL+C+VCLSKFE +EILRLLPKC+HAFHI CID
Sbjct: 93  DRFSGLDKTAIESLPLFRFSALKGSKQGLDCSVCLSKFESVEILRLLPKCRHAFHIGCID 152

Query: 152 HWLEKHASCPLCRQRV-CSEDLKLLTGSSSMRFLWSNLSELKQDSNIELFVQREEEEQ-- 211
            WLE+HA+CPLCR RV   ED  +LT  +S RFL  N SE+++DS++EL+++REEEE+  
Sbjct: 153 QWLEQHATCPLCRDRVSMEEDSSVLTNGNSFRFL--NQSEIREDSSLELYIEREEEEERI 212

Query: 212 --EQLHGSSRFSIGRSFRKILKSDKEGEMLIQKACDGDENMKNLHRHNHKIILSDFVFMN 271
             E+L GSSRFSIG SFRKILK   + + L+ +  +  +  K +H+ NH+I++SD VF N
Sbjct: 213 HREELSGSSRFSIGESFRKILKLGNKEKTLLDEHVNDKDEKKLMHKFNHRIVVSDVVFKN 272

Query: 272 RWSNVSSSDLMFLNKEMVGSISSSRFTSLETDIEQSTLSRSVQNEE--ILKIKEEMDIKR 331
           RWSNVSSSDLMFLN EMV SISS RF+SL+       + R  + ++  IL+IKEEM+ KR
Sbjct: 273 RWSNVSSSDLMFLNSEMVNSISSERFSSLD------HVKRGDEEDQIGILRIKEEMEAKR 332

Query: 332 SFESKLSTISQSNSVPILEFPSTSESAANPCQTARITSTEARRSVSEITGISRFGGDLHM 391
             E+KL++++   S         SE+  +  ++  +     RRSVS+IT + R    +H 
Sbjct: 333 MLENKLTSMTTMFS---------SENGDSGSKSRSVMIEPGRRSVSDITAVPRLSISIHG 392

Query: 392 KFN-RKVEIGESSDLGNNVKQERMRQIWYPIAKRTVQWFANRETRFQ 420
             +    E   +   G N  +ER R++W PIA++T QWFANRE R Q
Sbjct: 393 DCSGSAAETASALQNGGNETEERRRRLWLPIARKTAQWFANREKRSQ 422

BLAST of Lag0034464 vs. TAIR 10
Match: AT2G20030.1 (RING/U-box superfamily protein )

HSP 1 Score: 280.0 bits (715), Expect = 3.2e-75
Identity = 178/409 (43.52%), Postives = 239/409 (58.44%), Query Frame = 0

Query: 27  AENSAFQPSLGFVIGILGVMFLLTFILLVYAKFCHR----RSSIRGDNVNH----LGQIR 86
           A +  F+PSL  + G+  ++F LTF+LLVYAK  H      +   G+ + H     G   
Sbjct: 32  ATSDLFKPSLAIITGVFSIVFTLTFVLLVYAKCFHNDLRSETDSDGERIRHDRLWQGLFN 91

Query: 87  SSSRFSGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSKFEDIEILRLLPKCKHAFHINC 146
            SSRFSG+DK  IESLPFFRFS LKG K+GLEC+VCLSKFED+EILRLLPKC+HAFHI C
Sbjct: 92  RSSRFSGLDKKAIESLPFFRFSALKGLKQGLECSVCLSKFEDVEILRLLPKCRHAFHIGC 151

Query: 147 IDHWLEKHASCPLCRQRVCSEDLKLLTGSSSMRFLWSNLSELK-QDSNIELFVQREEEEQ 206
           ID WLE+HA+CPLCR RV  ED   + G+SS      N SE + +DS +E++++REE   
Sbjct: 152 IDQWLEQHATCPLCRNRVNIEDDLSVLGNSSTSLRILNQSETREEDSRLEIYIEREEGTN 211

Query: 207 EQLHGSSRFSIGRSFRKILKSDKEGEMLIQKACDGDENM---KNLHRHNHKIILSDFVFM 266
           +   GSSRFS   SFRKILK      +L+++  +G+EN+   K +H+ NH+I++SD VF 
Sbjct: 212 D---GSSRFS---SFRKILKK----SLLLER--EGNENIDEKKLMHKFNHRIVVSDAVFK 271

Query: 267 NRWSNVSSSDLMFLNKEMVGSISSSRFTSLETDIEQSTLSRSVQNEEILKIKEEMDIKRS 326
           NRWSN++SSDL FL  EM+ S+SS RF+S++             +   L+ KE+M++KR 
Sbjct: 272 NRWSNITSSDLTFLTSEMLNSVSSDRFSSVDR-----------VHRGNLRDKEDMEMKRM 331

Query: 327 FESKLSTISQSNSVPILEFPSTSESAANPCQTARITSTEARRSVSEITGISR---FGGDL 386
                  I   +S                          +RR+VSEIT +SR    GG  
Sbjct: 332 L------IKHKDS--------------------------SRRTVSEITTVSREKAVGGSY 376

Query: 387 HMKFNRKVEIGESSDLGNN-----VKQERMRQIWYPIAKRTVQWFANRE 416
                     G ++    N       +ER R++W PIA+RT QWF NRE
Sbjct: 392 R---------GSTASTSQNYAVTATTEERRRRLWLPIARRTAQWFVNRE 376

BLAST of Lag0034464 vs. TAIR 10
Match: AT5G05810.1 (RING/U-box superfamily protein )

HSP 1 Score: 152.9 bits (385), Expect = 5.8e-37
Identity = 106/293 (36.18%), Postives = 145/293 (49.49%), Query Frame = 0

Query: 34  PSLGFVIGILGVMFLLTFILLVYAKFCHRRS-------------SIRGDNVNHLGQIRSS 93
           P +  VI +L   F LTF+LL+Y K C RR+             +  G    + G +   
Sbjct: 2   PGIAVVIAVLTAFFSLTFLLLLYVKHCKRRNGSVYVNHPQRFAITRYGGGYYNGGGVVGG 61

Query: 94  SRFSGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSKFEDIEILRLLPKCKHAFHINCID 153
            + SGID++VIESLP FRF  L G K+GLECAVCL++FE  E+LRLLPKCKHAFH+ C+D
Sbjct: 62  RKNSGIDRSVIESLPVFRFGALSGHKDGLECAVCLARFEPTEVLRLLPKCKHAFHVECVD 121

Query: 154 HWLEKHASCPLCRQRVCSEDLKLLTGSSSMRFLWSNLSELKQDSN--------IELFV-- 213
            WL+ H++CPLCR RV  ED+ L+   +S    W  L   K +SN        +  F+  
Sbjct: 122 TWLDAHSTCPLCRYRVDPEDILLIGDCNS----WFELQFSKDESNSVNNNPPGLTRFIPV 181

Query: 214 -----------QREEEEQEQLHGSSRFSIGRSFRKILKS--------DKEGEMLIQKACD 273
                      +R     E    SS  S   SFR+ L S        +++ E +     D
Sbjct: 182 SRISGRHSSAGERASRLNEIRTSSSYKSNPMSFRRSLDSSLKVNDAGEEKSESVAVNCLD 241

BLAST of Lag0034464 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 112.5 bits (280), Expect = 8.7e-25
Identity = 56/136 (41.18%), Postives = 87/136 (63.97%), Query Frame = 0

Query: 27  AENSAFQPSLGFVIGILGVMFLLTFILLVYAKFCHRR---SSIRGDNVNHLGQ------I 86
           + +S+F P L  +IGIL    +L     + +K+CHR    SS    N+NH G+       
Sbjct: 49  SSSSSFSPLLIALIGILTSALILVSYYTLISKYCHRHHQTSSSETLNLNHNGEGFFSSTQ 108

Query: 87  RSSSRFSGIDKTVIESLPFFRFSTLKGSKEGLECAVCLSKFEDIEILRLLPKCKHAFHIN 146
           R S+   G+++++I+S+  +++ +  G  +G +C+VCLS+FE+ E LRLLPKC HAFH+ 
Sbjct: 109 RISTNGDGLNESMIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLP 168

Query: 147 CIDHWLEKHASCPLCR 154
           CID WL+ H++CPLCR
Sbjct: 169 CIDTWLKSHSNCPLCR 184

BLAST of Lag0034464 vs. TAIR 10
Match: AT4G40070.1 (RING/U-box superfamily protein )

HSP 1 Score: 110.9 bits (276), Expect = 2.5e-24
Identity = 57/132 (43.18%), Postives = 77/132 (58.33%), Query Frame = 0

Query: 34  PSLGFVIGILGVMFLLTFILLVYAKFCHRRSSIRGDNVNHLGQIRSSSRFSGIDKTVIES 93
           PS   V  +L  +F LT +L VY + C R +               SSR  G+D  V+ES
Sbjct: 44  PSKTTVFAVLVTLFFLTGLLSVYIRHCARSNPDSSTRYFRNRANDGSSRRGGLDNAVVES 103

Query: 94  LPFFRFSTLKGSKEG---LECAVCLSKFEDIEILRLLPKCKHAFHINCIDHWLEKHASCP 153
            P F +S++K SK G   LECA+CL++ ED E +RLLP C H FHI+CID WL  HA+CP
Sbjct: 104 FPVFAYSSVKESKIGSKDLECAICLNELEDHETVRLLPICNHLFHIDCIDTWLYSHATCP 163

Query: 154 LCRQRVCSEDLK 163
           +CR  + ++  K
Sbjct: 164 VCRSNLTAKSNK 175

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022144841.18.6e-20085.98E3 ubiquitin-protein ligase ATL42-like [Momordica charantia][more]
XP_038878637.15.0e-19283.83E3 ubiquitin-protein ligase ATL42-like [Benincasa hispida][more]
XP_008462695.18.1e-19083.79PREDICTED: E3 ubiquitin-protein ligase ATL42-like [Cucumis melo] >KAA0046521.1 E... [more]
KAG6587983.11.7e-16372.11E3 ubiquitin-protein ligase ATL42, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022929946.11.7e-16372.11E3 ubiquitin-protein ligase ATL42-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q5XF851.1e-9650.37E3 ubiquitin-protein ligase ATL42 OS=Arabidopsis thaliana OX=3702 GN=ATL42 PE=1 ... [more]
Q9SL784.4e-7443.52Putative RING-H2 finger protein ATL12 OS=Arabidopsis thaliana OX=3702 GN=ATL12 P... [more]
Q5EAE92.2e-3636.03RING-H2 finger protein ATL43 OS=Arabidopsis thaliana OX=3702 GN=ATL43 PE=2 SV=2[more]
Q9LF641.2e-2341.18RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1[more]
Q8W5713.6e-2343.18RING-H2 finger protein ATL32 OS=Arabidopsis thaliana OX=3702 GN=ATL32 PE=2 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1CUL64.2e-20085.98E3 ubiquitin-protein ligase ATL42-like OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A5D3DZ483.9e-19083.79E3 ubiquitin-protein ligase ATL42-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3CHK33.9e-19083.79E3 ubiquitin-protein ligase ATL42-like OS=Cucumis melo OX=3656 GN=LOC103500995 P... [more]
A0A6J1EPJ88.2e-16472.11E3 ubiquitin-protein ligase ATL42-like OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A6J1KW641.4e-16372.44E3 ubiquitin-protein ligase ATL42-like OS=Cucurbita maxima OX=3661 GN=LOC1114992... [more]
Match NameE-valueIdentityDescription
AT4G28890.17.7e-9850.37RING/U-box superfamily protein [more]
AT2G20030.13.2e-7543.52RING/U-box superfamily protein [more]
AT5G05810.15.8e-3736.18RING/U-box superfamily protein [more]
AT5G17600.18.7e-2541.18RING/U-box superfamily protein [more]
AT4G40070.12.5e-2443.18RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 111..152
e-value: 1.2E-6
score: 38.1
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 110..153
e-value: 3.2E-12
score: 46.6
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 111..153
score: 12.622055
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 85..161
e-value: 1.1E-21
score: 77.8
NoneNo IPR availablePANTHERPTHR46539E3 UBIQUITIN-PROTEIN LIGASE ATL42coord: 22..422
NoneNo IPR availablePANTHERPTHR46539:SF1E3 UBIQUITIN-PROTEIN LIGASE ATL42coord: 22..422
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 110..153
e-value: 5.39141E-20
score: 80.8089
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 90..157

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0034464.1Lag0034464.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane