Lag0034413 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0034413
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionHeavy metal-associated domain containing protein
Locationchr3: 7143868 .. 7144478 (-)
RNA-Seq ExpressionLag0034413
SyntenyLag0034413
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGAAGCTGCAGAAGAAAGTTGCAAAGGTTTTGGATTGTTTCAGTACTCCTTCATCATGTGGCTCCAATTCTTGTTTTTGCATAAACTCCATGGAAGATCAAGAAGATGAGTTTGATAAAGAGCCACTCATCCCCACTGAGAAGGATCACTTGCTGACATTGAAGGATGTTGTCAATGGAAACCAGACCTTGGCCTTCCAACTCAAACCCAAGGTAATTTTATTTCTACCCATTAAGTTTGAAAAATAATTATTTGGGTATAAATTCTTTTTTTGGATTTTTGTTTTGATTTTCCAGATGGTGACTTTGAGGGTTTCCATGCACTGTAGAGGCTGTGCTAAGAAAGTGGAGAAACACATCGCAAAGATGGAAGGTCGGAAAAATTATCCAATGAAACCGTCACAATAATAATATGGAATATAAATTCCAAGATTTATAATGGATTTTGTTTTGTTTTGTTTTTTTTTTAATGAACAGGAGTGAGCTCGTACACAATAGACTTGGAGACTAAGATGGTGATTATTATTGGAGACATTCTGCCTTATGAAGTGGTGGAGAGTGTCTCCAAAGTCAAAAACGCACAGCTCTGGCAATCTTCGCTAGCTTGA

mRNA sequence

ATGGGGAAGCTGCAGAAGAAAGTTGCAAAGGTTTTGGATTGTTTCAGTACTCCTTCATCATGTGGCTCCAATTCTTGTTTTTGCATAAACTCCATGGAAGATCAAGAAGATGAGTTTGATAAAGAGCCACTCATCCCCACTGAGAAGGATCACTTGCTGACATTGAAGGATGTTGTCAATGGAAACCAGACCTTGGCCTTCCAACTCAAACCCAAGATGGTGACTTTGAGGGTTTCCATGCACTGTAGAGGCTGTGCTAAGAAAGTGGAGAAACACATCGCAAAGATGGAAGGAGTGAGCTCGTACACAATAGACTTGGAGACTAAGATGGTGATTATTATTGGAGACATTCTGCCTTATGAAGTGGTGGAGAGTGTCTCCAAAGTCAAAAACGCACAGCTCTGGCAATCTTCGCTAGCTTGA

Coding sequence (CDS)

ATGGGGAAGCTGCAGAAGAAAGTTGCAAAGGTTTTGGATTGTTTCAGTACTCCTTCATCATGTGGCTCCAATTCTTGTTTTTGCATAAACTCCATGGAAGATCAAGAAGATGAGTTTGATAAAGAGCCACTCATCCCCACTGAGAAGGATCACTTGCTGACATTGAAGGATGTTGTCAATGGAAACCAGACCTTGGCCTTCCAACTCAAACCCAAGATGGTGACTTTGAGGGTTTCCATGCACTGTAGAGGCTGTGCTAAGAAAGTGGAGAAACACATCGCAAAGATGGAAGGAGTGAGCTCGTACACAATAGACTTGGAGACTAAGATGGTGATTATTATTGGAGACATTCTGCCTTATGAAGTGGTGGAGAGTGTCTCCAAAGTCAAAAACGCACAGCTCTGGCAATCTTCGCTAGCTTGA

Protein sequence

MGKLQKKVAKVLDCFSTPSSCGSNSCFCINSMEDQEDEFDKEPLIPTEKDHLLTLKDVVNGNQTLAFQLKPKMVTLRVSMHCRGCAKKVEKHIAKMEGVSSYTIDLETKMVIIIGDILPYEVVESVSKVKNAQLWQSSLA
Homology
BLAST of Lag0034413 vs. NCBI nr
Match: KAG6587944.1 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 247.3 bits (630), Expect = 7.9e-62
Identity = 125/143 (87.41%), Postives = 131/143 (91.61%), Query Frame = 0

Query: 1   MGKLQKKVAKVLDCFST---PSSCGSNSCFCINSMEDQEDEFDKEPLIPTEKDHLLTLKD 60
           MGKL KK++K LDCFS    PSSC S SCFCINSME+QEDEFDK PLIPT KD L TLKD
Sbjct: 1   MGKLHKKISKALDCFSLSLGPSSCSSGSCFCINSMEEQEDEFDKLPLIPTHKDQLPTLKD 60

Query: 61  VVNGNQTLAFQLKPKMVTLRVSMHCRGCAKKVEKHIAKMEGVSSYTIDLETKMVIIIGDI 120
           VVNGNQTLAFQLKPKMVTLRVSMHCRGCA+KVEKHI+KMEGVSSYTIDLETKMVIIIGDI
Sbjct: 61  VVNGNQTLAFQLKPKMVTLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVIIIGDI 120

Query: 121 LPYEVVESVSKVKNAQLWQSSLA 141
           LP+EVVESVSKVKNAQLWQSSLA
Sbjct: 121 LPFEVVESVSKVKNAQLWQSSLA 143

BLAST of Lag0034413 vs. NCBI nr
Match: XP_023531514.1 (heavy metal-associated isoprenylated plant protein 45-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 245.4 bits (625), Expect = 3.0e-61
Identity = 123/143 (86.01%), Postives = 131/143 (91.61%), Query Frame = 0

Query: 1   MGKLQKKVAKVLDCFST---PSSCGSNSCFCINSMEDQEDEFDKEPLIPTEKDHLLTLKD 60
           MGKL KK++K LDCFS    PSSC S SCFCINSME+QEDEFDK PLIP+ KD L TLKD
Sbjct: 1   MGKLHKKISKALDCFSLSLGPSSCSSGSCFCINSMEEQEDEFDKLPLIPSHKDQLPTLKD 60

Query: 61  VVNGNQTLAFQLKPKMVTLRVSMHCRGCAKKVEKHIAKMEGVSSYTIDLETKMVIIIGDI 120
           VVNGNQTLAFQLKPKMVTLRVSMHCRGCA+KVEKHI+KMEGVSSYTIDLETKMVIIIGD+
Sbjct: 61  VVNGNQTLAFQLKPKMVTLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVIIIGDV 120

Query: 121 LPYEVVESVSKVKNAQLWQSSLA 141
           LP+EVVESVSKVKNAQLWQSSLA
Sbjct: 121 LPFEVVESVSKVKNAQLWQSSLA 143

BLAST of Lag0034413 vs. NCBI nr
Match: XP_022973748.1 (heavy metal-associated isoprenylated plant protein 45-like [Cucurbita maxima])

HSP 1 Score: 244.2 bits (622), Expect = 6.7e-61
Identity = 124/143 (86.71%), Postives = 130/143 (90.91%), Query Frame = 0

Query: 1   MGKLQKKVAKVLDCFST---PSSCGSNSCFCINSMEDQEDEFDKEPLIPTEKDHLLTLKD 60
           MGKL KK++K LDCFS    PSSC S SCFCINSME+QEDEFDK PLI T KD L TLKD
Sbjct: 1   MGKLHKKISKALDCFSLSLGPSSCSSGSCFCINSMEEQEDEFDKLPLIATHKDQLPTLKD 60

Query: 61  VVNGNQTLAFQLKPKMVTLRVSMHCRGCAKKVEKHIAKMEGVSSYTIDLETKMVIIIGDI 120
           VVNGNQTLAFQLKPKMVTLRVSMHCRGCA+KVEKHI+KMEGVSSYTIDLETKMVIIIGDI
Sbjct: 61  VVNGNQTLAFQLKPKMVTLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVIIIGDI 120

Query: 121 LPYEVVESVSKVKNAQLWQSSLA 141
           LP+EVVESVSKVKNAQLWQSSLA
Sbjct: 121 LPFEVVESVSKVKNAQLWQSSLA 143

BLAST of Lag0034413 vs. NCBI nr
Match: XP_022933309.1 (heavy metal-associated isoprenylated plant protein 45-like [Cucurbita moschata])

HSP 1 Score: 244.2 bits (622), Expect = 6.7e-61
Identity = 124/143 (86.71%), Postives = 130/143 (90.91%), Query Frame = 0

Query: 1   MGKLQKKVAKVLDCFST---PSSCGSNSCFCINSMEDQEDEFDKEPLIPTEKDHLLTLKD 60
           MGKL KK++K LDCFS    PSSC S SCFCINSME+QEDEFDK PLI T KD L TLKD
Sbjct: 1   MGKLHKKISKALDCFSLSLGPSSCSSGSCFCINSMEEQEDEFDKLPLISTHKDQLPTLKD 60

Query: 61  VVNGNQTLAFQLKPKMVTLRVSMHCRGCAKKVEKHIAKMEGVSSYTIDLETKMVIIIGDI 120
           VVNGNQTLAFQLKPKMVTLRVSMHCRGCA+KVEKHI+KMEGVSSYTIDLETKMVIIIGDI
Sbjct: 61  VVNGNQTLAFQLKPKMVTLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVIIIGDI 120

Query: 121 LPYEVVESVSKVKNAQLWQSSLA 141
           LP+EVVESVSKVKNAQLWQSSLA
Sbjct: 121 LPFEVVESVSKVKNAQLWQSSLA 143

BLAST of Lag0034413 vs. NCBI nr
Match: XP_022144911.1 (protein SODIUM POTASSIUM ROOT DEFECTIVE 1 [Momordica charantia])

HSP 1 Score: 226.9 bits (577), Expect = 1.1e-55
Identity = 119/145 (82.07%), Postives = 129/145 (88.97%), Query Frame = 0

Query: 1   MGKLQKKVAKVLDCFSTPSSCGSNSCFCINSMED-QEDEFDKEPLIP----TEKDHLLTL 60
           MGK  KK+A VLDCFS+ SSC  +SCFCINSMED QEDEFDKEPL+P     +K+  L L
Sbjct: 3   MGKKLKKIASVLDCFSSASSC--SSCFCINSMEDQQEDEFDKEPLMPLPTHNQKEQSLRL 62

Query: 61  KDVVNGNQTLAFQLKPKMVTLRVSMHCRGCAKKVEKHIAKMEGVSSYTIDLETKMVIIIG 120
           KD+VNGNQTLAFQLKPKMV LRVSMHCRGCA+KVEKHI+KMEGVSSYTIDLETKMVIIIG
Sbjct: 63  KDLVNGNQTLAFQLKPKMVMLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVIIIG 122

Query: 121 DILPYEVVESVSKVKNAQLWQSSLA 141
           DILP+EVVESVSKVKNAQLWQSSLA
Sbjct: 123 DILPFEVVESVSKVKNAQLWQSSLA 145

BLAST of Lag0034413 vs. ExPASy Swiss-Prot
Match: Q58FZ0 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 2 OS=Arabidopsis thaliana OX=3702 GN=NAKR2 PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 8.0e-17
Identity = 46/107 (42.99%), Postives = 63/107 (58.88%), Query Frame = 0

Query: 29  INSMEDQEDEFDKEPLIPTEKDHLLTLKDVVNGNQTLAFQLKPKMVTLRVSMHCRGCAKK 88
           I    DQ+     E   P  +D  LT K    G+         ++V L+VS+HCRGC  K
Sbjct: 144 ITGSVDQDPAKTVEAEAPAGEDKTLTEKKTTCGD------TDQQVVVLKVSLHCRGCEGK 203

Query: 89  VEKHIAKMEGVSSYTIDLETKMVIIIGDILPYEVVESVSKVKNAQLW 136
           V KH+A+M+GV+S+ ID   K V + GDI P E+++S+SKVKNAQ W
Sbjct: 204 VRKHLARMQGVTSFNIDFAAKKVTVTGDITPLEILDSISKVKNAQFW 244

BLAST of Lag0034413 vs. ExPASy Swiss-Prot
Match: Q8LDS4 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=NAKR1 PE=1 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 2.0e-15
Identity = 36/64 (56.25%), Postives = 50/64 (78.12%), Query Frame = 0

Query: 72  KMVTLRVSMHCRGCAKKVEKHIAKMEGVSSYTIDLETKMVIIIGDILPYEVVESVSKVKN 131
           ++V LRVS+HC+GCA KV+KH++K++GV+SY ID   K V + GD+ P  V+ S+SKVKN
Sbjct: 250 QVVVLRVSLHCKGCAGKVKKHLSKLKGVTSYNIDFAAKKVTVTGDVTPLTVLASISKVKN 309

Query: 132 AQLW 136
           AQ W
Sbjct: 310 AQFW 313

BLAST of Lag0034413 vs. ExPASy Swiss-Prot
Match: Q8RXH8 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 3 OS=Arabidopsis thaliana OX=3702 GN=NAKR3 PE=2 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.1e-13
Identity = 37/66 (56.06%), Postives = 50/66 (75.76%), Query Frame = 0

Query: 72  KMVTLRVSM--HCRGCAKKVEKHIAKMEGVSSYTIDLETKMVIIIGDILPYEVVESVSKV 131
           ++V LRVS+  HCRGC  KV+KH++KM+GV+S+ ID  +K V + GDI P EV+  +SKV
Sbjct: 168 QVVVLRVSLHCHCRGCQGKVKKHLSKMQGVTSFNIDFASKKVTVTGDITPLEVLGCLSKV 227

Query: 132 KNAQLW 136
           KNAQ W
Sbjct: 228 KNAQFW 233

BLAST of Lag0034413 vs. ExPASy Swiss-Prot
Match: Q84J88 (Heavy metal-associated isoprenylated plant protein 36 OS=Arabidopsis thaliana OX=3702 GN=HIPP36 PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 1.5e-07
Identity = 24/65 (36.92%), Postives = 48/65 (73.85%), Query Frame = 0

Query: 76  LRVSMHCRGCAKKVEKHIAKMEGVSSYTIDLETKMVIIIGDILPYEVVESVSKV-KNAQL 135
           LRVS+HC GC +K++K ++K++GV +  ID++ + V +IG++ P  +++ + K  ++A+L
Sbjct: 34  LRVSIHCEGCKRKIKKILSKIDGVYTTNIDVKQQKVTVIGNVEPEILIKKIMKAGRHAEL 93

Query: 136 WQSSL 140
           W +S+
Sbjct: 94  WPTSM 98

BLAST of Lag0034413 vs. ExPASy Swiss-Prot
Match: Q94BT9 (Copper transport protein ATX1 OS=Arabidopsis thaliana OX=3702 GN=ATX1 PE=1 SV=2)

HSP 1 Score: 52.8 bits (125), Expect = 3.7e-06
Identity = 24/65 (36.92%), Postives = 42/65 (64.62%), Query Frame = 0

Query: 74  VTLRVSMHCRGCAKKVEKHIAKMEGVSSYTIDLETKMVIIIGDILPYEVVESVSKV-KNA 133
           V LRV+M C GC   V++ + KMEGV S+ +D++ + V + G++ P  V+++V+K  K  
Sbjct: 5   VVLRVAMTCEGCVGAVKRVLGKMEGVESFDVDIKEQKVTVKGNVQPDAVLQTVTKTGKKT 64

Query: 134 QLWQS 138
             W++
Sbjct: 65  AFWEA 69

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6587944.17.9e-6287.41Protein SODIUM POTASSIUM ROOT DEFECTIVE 1, partial [Cucurbita argyrosperma subsp... [more]
XP_023531514.13.0e-6186.01heavy metal-associated isoprenylated plant protein 45-like [Cucurbita pepo subsp... [more]
XP_022973748.16.7e-6186.71heavy metal-associated isoprenylated plant protein 45-like [Cucurbita maxima][more]
XP_022933309.16.7e-6186.71heavy metal-associated isoprenylated plant protein 45-like [Cucurbita moschata][more]
XP_022144911.11.1e-5582.07protein SODIUM POTASSIUM ROOT DEFECTIVE 1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q58FZ08.0e-1742.99Protein SODIUM POTASSIUM ROOT DEFECTIVE 2 OS=Arabidopsis thaliana OX=3702 GN=NAK... [more]
Q8LDS42.0e-1556.25Protein SODIUM POTASSIUM ROOT DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=NAK... [more]
Q8RXH81.1e-1356.06Protein SODIUM POTASSIUM ROOT DEFECTIVE 3 OS=Arabidopsis thaliana OX=3702 GN=NAK... [more]
Q84J881.5e-0736.92Heavy metal-associated isoprenylated plant protein 36 OS=Arabidopsis thaliana OX... [more]
Q94BT93.7e-0636.92Copper transport protein ATX1 OS=Arabidopsis thaliana OX=3702 GN=ATX1 PE=1 SV=2[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006121Heavy metal-associated domain, HMAPFAMPF00403HMAcoord: 75..128
e-value: 3.3E-14
score: 53.1
IPR006121Heavy metal-associated domain, HMAPROSITEPS50846HMA_2coord: 79..129
score: 11.153432
IPR006121Heavy metal-associated domain, HMACDDcd00371HMAcoord: 75..116
e-value: 1.53364E-11
score: 54.1489
NoneNo IPR availableGENE3D3.30.70.100coord: 70..133
e-value: 2.0E-16
score: 62.2
NoneNo IPR availablePANTHERPTHR46119:SF11HEAVY METAL TRANSPORT/DETOXIFICATION SUPERFAMILY PROTEINcoord: 7..137
IPR044526Protein SODIUM POTASSIUM ROOT DEFECTIVE 1/2/3PANTHERPTHR46119OS08G0405700 PROTEINcoord: 7..137
IPR036163Heavy metal-associated domain superfamilySUPERFAMILY55008HMA, heavy metal-associated domaincoord: 71..129

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0034413.1Lag0034413.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding