Lag0034367 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0034367
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCytochrome P450
Locationchr3: 6698421 .. 6700868 (-)
RNA-Seq ExpressionLag0034367
SyntenyLag0034367
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGGAGAATCCAGCGGCGGCGCCGGCGCCTTTAGAATCAGTTAAAGTTCCAGTGCTGTGCGCCGTGGTGGTGATTTTATGTGCAAAAATGGCGGTGGCGCTATGGTGGAAGCCGCGGAGAATCGAAGAGCACTTTCTGAGACAAGGAATTCGAGGCCCTCCTTATCGTTTCTTCATCGGTAACTTCAAAGAACTCGTCTCTATGATGCTCGCTGCTTCTTCCCATCCAATGCCCAATTTCTCCCACAATATTCTCCCCCGTGTTCTTTCCTTCTACCATCACTGGAAGAAAATTTACGGTAACAACCAGACATTCAAACTTCTAACCTCTTTTATAAACTGCAGAGTCGGAGTTGACTCTGGCTTGATTTTGTAGGTTCGACATTTCTGATCTGGTTCGGACCCATCGTTCGAGTGGCGGTGTCGGACCCGGACATGATCCGAGAAATCTTGACCACCAACTCCGATTTCTACGAGAAGAACGAAGCTCATCCTCTCGTCAAACAGCTCGAAGGGGACGGCCTCCTCACCCTCAAGGATCAAAAATGGGCTCTCCATCGCAAAATCATCTCCCCCACTTTTCACATGGAGAATCTCAAAGTAAAAAAATGATTTTATATCGTTGAATGTACGAGATAGAAATTAAAAATCAAAACTTTATTCGATATTTTATGTGGGGTTACGTTGTTGGTGTGCAGTTGCTGATTCCGGTGGTGGAGGAAAGTGTGGTGGATATGGTCGAGAAATGGTCGGCCATGGCGGACGACACGGCCTCCGGCGAGGTCGAGATCGAAGTTTTCGATTGCTTCCAAACCCTAACGGAAGATGTCATAACAAAAACAGCATTTGGGAGTAGCTATGAAGATGGAAAAGCGATTTTCAAGCTTCAAACTCAACAAATGATATTAGCATCCGAAGCTTTTCAGAAGGTCTTCATTCCTGGCTATAGGTACAATTACAATTACAATTTACAATTAGAATTATAAATACAATTATTGTTGTTTTTAAAGAAAAAATCTTTTTGGGGTTTAATTTTCAGATTTCTTCCGACAAGAACGAATATCAGTTCGTGGAAATTGGACAAGGAAATAAAGAAATCGTTGATGAAATTGATCGAGGGTCGAAAAGGGAATTCAGAGAAAAATTGGGCAATGGATGAAAAAGGGCCAAAAGATTTATTAGGGCTGATGATTCAAGCTTCCAACTCATCTTCTTGTTCTTCATTAAACAATAATAATATTACCGTCCACGACATTGTTGAAGAGTGCAAAAGCTTCTTCTTTGCTGGCAAACAGACCACTTCCAATCTGCTGACGTGGACTACTGTCTTGCTGGCAATGCATCCACAGTGGCAAATCCAGGCACGTGACGAGGTCCTTAGCGTGTGCGGTCCACGTGCCCTCCCCTCCAGAGATGATGTTTCTAAGTTGAAGATCGTAAGCACTTTTCTTTTTCTTTTTCTTTTTCTTTTTCCTATATATTTCTATTTTATCATTATTTAAAAAAAGAAAAAATGAAAAATGGTAATAACGACTTATGGATTATATTGATAAGAATATGTGATGGATTAAAATAAGACCCACCAGTCATTTATTTATTATGATCACGCAACGTTATGTGTACTAAATTTATGGATATTATAAAACCCAATATAATATTAACAAAATATTGGATTGTGCCATTCATAAGGAGTGGTGTGGATTTAATTTTGTTTATGTTAATTAATTATAGTACATGTTATTGTTAGGGGCAGTGTTTTAAATTGGATTAAAGAAATATGGTAGTTTAGAAATTTTGTTTTTGTCATTTAGTTTGGTGTTTTTGTTTCTTTGCTAAGAAAAACTAAACTGCCAACTTTTGCTTTACATTGCTTGGTTTTTTGATAGCAACTTCTTGTTTCTTCTTTGTCATTTTGGATACAACATTTTCAGCCCCTTCCTACTTACCAAAACTTCTTTATTTTTGTTTGGGGAATCATTTGGTGTGGCAGTTGAGCATGATACTAAACGAGTCCCTACGATTGTACCCGCCCACTGTCGCTACCATTAGACGTGCAAAGGCTGACGTGGAGCTAGGAGGGCGGATGATTCCGCGTGGGACGGAGTTTTTGATTCCAATTCTGGCCGTCCATCACGATCAAAGTATTTGGGGGAATGATGCGAGTGAGTTTAATCCAGGGCGATTCTCAGAAGGAGTGGGTCGTGCTGGAAATCATCCAGCAGGGTTCATTCCGTTTGGACTTGGTGTTCGTACTTGCATTGGCCAAAACTTGGCCCTTTTACAAGCAAAGTTGGCAATGGCAATCATACTTCAACGATTCTCGTTTCGATTGGCTCCCACCTATCAGCATGCACCAACGGTCTTGATGCTTCTTTATCCACAATATGGGGCCCCCATTATTTTTCAAAAGATATCCCAATTCACAGGCGATGCTTCCATGTCATAG

mRNA sequence

ATGAAGGAGAATCCAGCGGCGGCGCCGGCGCCTTTAGAATCAGTTAAAGTTCCAGTGCTGTGCGCCGTGGTGGTGATTTTATGTGCAAAAATGGCGGTGGCGCTATGGTGGAAGCCGCGGAGAATCGAAGAGCACTTTCTGAGACAAGGAATTCGAGGCCCTCCTTATCGTTTCTTCATCGGTAACTTCAAAGAACTCGTCTCTATGATGCTCGCTGCTTCTTCCCATCCAATGCCCAATTTCTCCCACAATATTCTCCCCCGTGTTCTTTCCTTCTACCATCACTGGAAGAAAATTTACGGTTCGACATTTCTGATCTGGTTCGGACCCATCGTTCGAGTGGCGGTGTCGGACCCGGACATGATCCGAGAAATCTTGACCACCAACTCCGATTTCTACGAGAAGAACGAAGCTCATCCTCTCGTCAAACAGCTCGAAGGGGACGGCCTCCTCACCCTCAAGGATCAAAAATGGGCTCTCCATCGCAAAATCATCTCCCCCACTTTTCACATGGAGAATCTCAAATTGCTGATTCCGGTGGTGGAGGAAAGTGTGGTGGATATGGTCGAGAAATGGTCGGCCATGGCGGACGACACGGCCTCCGGCGAGGTCGAGATCGAAGTTTTCGATTGCTTCCAAACCCTAACGGAAGATGTCATAACAAAAACAGCATTTGGGAGTAGCTATGAAGATGGAAAAGCGATTTTCAAGCTTCAAACTCAACAAATGATATTAGCATCCGAAGCTTTTCAGAAGGTCTTCATTCCTGGCTATAGATTTCTTCCGACAAGAACGAATATCAGTTCGTGGAAATTGGACAAGGAAATAAAGAAATCGTTGATGAAATTGATCGAGGGTCGAAAAGGGAATTCAGAGAAAAATTGGGCAATGGATGAAAAAGGGCCAAAAGATTTATTAGGGCTGATGATTCAAGCTTCCAACTCATCTTCTTGTTCTTCATTAAACAATAATAATATTACCGTCCACGACATTGTTGAAGAGTGCAAAAGCTTCTTCTTTGCTGGCAAACAGACCACTTCCAATCTGCTGACGTGGACTACTGTCTTGCTGGCAATGCATCCACAGTGGCAAATCCAGGCACGTGACGAGGTCCTTAGCGTGTGCGGTCCACGTGCCCTCCCCTCCAGAGATGATGTTTCTAAGTTGAAGATCTTGAGCATGATACTAAACGAGTCCCTACGATTGTACCCGCCCACTGTCGCTACCATTAGACGTGCAAAGGCTGACGTGGAGCTAGGAGGGCGGATGATTCCGCGTGGGACGGAGTTTTTGATTCCAATTCTGGCCGTCCATCACGATCAAAGTATTTGGGGGAATGATGCGAGTGAGTTTAATCCAGGGCGATTCTCAGAAGGAGTGGGTCGTGCTGGAAATCATCCAGCAGGGTTCATTCCGTTTGGACTTGGTGTTCGTACTTGCATTGGCCAAAACTTGGCCCTTTTACAAGCAAAGTTGGCAATGGCAATCATACTTCAACGATTCTCGTTTCGATTGGCTCCCACCTATCAGCATGCACCAACGGTCTTGATGCTTCTTTATCCACAATATGGGGCCCCCATTATTTTTCAAAAGATATCCCAATTCACAGGCGATGCTTCCATGTCATAG

Coding sequence (CDS)

ATGAAGGAGAATCCAGCGGCGGCGCCGGCGCCTTTAGAATCAGTTAAAGTTCCAGTGCTGTGCGCCGTGGTGGTGATTTTATGTGCAAAAATGGCGGTGGCGCTATGGTGGAAGCCGCGGAGAATCGAAGAGCACTTTCTGAGACAAGGAATTCGAGGCCCTCCTTATCGTTTCTTCATCGGTAACTTCAAAGAACTCGTCTCTATGATGCTCGCTGCTTCTTCCCATCCAATGCCCAATTTCTCCCACAATATTCTCCCCCGTGTTCTTTCCTTCTACCATCACTGGAAGAAAATTTACGGTTCGACATTTCTGATCTGGTTCGGACCCATCGTTCGAGTGGCGGTGTCGGACCCGGACATGATCCGAGAAATCTTGACCACCAACTCCGATTTCTACGAGAAGAACGAAGCTCATCCTCTCGTCAAACAGCTCGAAGGGGACGGCCTCCTCACCCTCAAGGATCAAAAATGGGCTCTCCATCGCAAAATCATCTCCCCCACTTTTCACATGGAGAATCTCAAATTGCTGATTCCGGTGGTGGAGGAAAGTGTGGTGGATATGGTCGAGAAATGGTCGGCCATGGCGGACGACACGGCCTCCGGCGAGGTCGAGATCGAAGTTTTCGATTGCTTCCAAACCCTAACGGAAGATGTCATAACAAAAACAGCATTTGGGAGTAGCTATGAAGATGGAAAAGCGATTTTCAAGCTTCAAACTCAACAAATGATATTAGCATCCGAAGCTTTTCAGAAGGTCTTCATTCCTGGCTATAGATTTCTTCCGACAAGAACGAATATCAGTTCGTGGAAATTGGACAAGGAAATAAAGAAATCGTTGATGAAATTGATCGAGGGTCGAAAAGGGAATTCAGAGAAAAATTGGGCAATGGATGAAAAAGGGCCAAAAGATTTATTAGGGCTGATGATTCAAGCTTCCAACTCATCTTCTTGTTCTTCATTAAACAATAATAATATTACCGTCCACGACATTGTTGAAGAGTGCAAAAGCTTCTTCTTTGCTGGCAAACAGACCACTTCCAATCTGCTGACGTGGACTACTGTCTTGCTGGCAATGCATCCACAGTGGCAAATCCAGGCACGTGACGAGGTCCTTAGCGTGTGCGGTCCACGTGCCCTCCCCTCCAGAGATGATGTTTCTAAGTTGAAGATCTTGAGCATGATACTAAACGAGTCCCTACGATTGTACCCGCCCACTGTCGCTACCATTAGACGTGCAAAGGCTGACGTGGAGCTAGGAGGGCGGATGATTCCGCGTGGGACGGAGTTTTTGATTCCAATTCTGGCCGTCCATCACGATCAAAGTATTTGGGGGAATGATGCGAGTGAGTTTAATCCAGGGCGATTCTCAGAAGGAGTGGGTCGTGCTGGAAATCATCCAGCAGGGTTCATTCCGTTTGGACTTGGTGTTCGTACTTGCATTGGCCAAAACTTGGCCCTTTTACAAGCAAAGTTGGCAATGGCAATCATACTTCAACGATTCTCGTTTCGATTGGCTCCCACCTATCAGCATGCACCAACGGTCTTGATGCTTCTTTATCCACAATATGGGGCCCCCATTATTTTTCAAAAGATATCCCAATTCACAGGCGATGCTTCCATGTCATAG

Protein sequence

MKENPAAAPAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREILTTNSDFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSAMADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGLMIQASNSSSCSSLNNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKADVELGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLAMAIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKISQFTGDASMS
Homology
BLAST of Lag0034367 vs. NCBI nr
Match: XP_023006493.1 (cytochrome P450 734A1-like [Cucurbita maxima])

HSP 1 Score: 946.4 bits (2445), Expect = 1.1e-271
Identity = 466/530 (87.92%), Postives = 494/530 (93.21%), Query Frame = 0

Query: 9   PAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVS 68
           P P +S+K+P LCA++ IL  KMAVALWWKPRRIE HFLRQGIRGPPYRFFIGNFKEL S
Sbjct: 6   PPPFDSIKIPFLCALLFILAVKMAVALWWKPRRIEAHFLRQGIRGPPYRFFIGNFKELDS 65

Query: 69  MMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREILTT 128
           MMLAA+S PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIR+ILTT
Sbjct: 66  MMLAATSRPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRDILTT 125

Query: 129 NSDFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDM 188
           NS+FYEKNEAHPL+K+LEGDGLLTLKDQKWALHRKIISP F+MENLKLLIPVVEESVVDM
Sbjct: 126 NSEFYEKNEAHPLIKKLEGDGLLTLKDQKWALHRKIISPVFNMENLKLLIPVVEESVVDM 185

Query: 189 VEKWSAMADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASE 248
           VEKWSAMA   AS EVEI+VFD FQTL EDV+TKT FGSSYEDGKAIFKLQTQQMILASE
Sbjct: 186 VEKWSAMAGGAASDEVEIDVFDWFQTLAEDVVTKTVFGSSYEDGKAIFKLQTQQMILASE 245

Query: 249 AFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGL 308
           AFQKVFIPGYRFLPTRTNI+SWKLDKEI+KSLMKLIE RKGNSEK WAMDEK PKDLLGL
Sbjct: 246 AFQKVFIPGYRFLPTRTNINSWKLDKEIRKSLMKLIESRKGNSEKIWAMDEKAPKDLLGL 305

Query: 309 MIQASNSSSCSSLNNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 368
           MIQASNSSS SS  + NI+VHDIVEECK+FFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR
Sbjct: 306 MIQASNSSSSSSSLHTNISVHDIVEECKTFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 365

Query: 369 DEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKADVELGGRMIPRGT 428
           DEVLSVCGPRALPSRDD+SK KILSMILNESLRLYPPTVATIRRAK  VEL GRMIPRGT
Sbjct: 366 DEVLSVCGPRALPSRDDLSKFKILSMILNESLRLYPPTVATIRRAKTVVELRGRMIPRGT 425

Query: 429 EFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALL 488
           EFLIPILAVHHDQ+IWGNDASEFNPGRFSEGVG AGNHPAGFIPFG G+R CIG+NLALL
Sbjct: 426 EFLIPILAVHHDQNIWGNDASEFNPGRFSEGVGHAGNHPAGFIPFGHGLRACIGKNLALL 485

Query: 489 QAKLAMAIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKISQFTGD 539
           QAKLA+AII+QRFSFRLAP+YQHAP VLMLLYPQYGAP++FQKI +   D
Sbjct: 486 QAKLAIAIIVQRFSFRLAPSYQHAPAVLMLLYPQYGAPVVFQKIVETRRD 535

BLAST of Lag0034367 vs. NCBI nr
Match: XP_023531517.1 (cytochrome P450 734A1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 945.3 bits (2442), Expect = 2.4e-271
Identity = 467/528 (88.45%), Postives = 497/528 (94.13%), Query Frame = 0

Query: 9   PAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVS 68
           P PL+S+K+P LCA++ IL  KMAVALWWKPRRIE HFLRQGIRGPPYRFFIGNFKEL S
Sbjct: 6   PPPLDSIKIPFLCALLFILAVKMAVALWWKPRRIEAHFLRQGIRGPPYRFFIGNFKELDS 65

Query: 69  MMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREILTT 128
           MMLAA+S PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIR+ILTT
Sbjct: 66  MMLAATSRPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRDILTT 125

Query: 129 NSDFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDM 188
           NS+FYEKNEAHPL+K+LEGDGLLTLKDQKWALHRKIISP F+MENLKLLIPVVEESVVDM
Sbjct: 126 NSEFYEKNEAHPLIKKLEGDGLLTLKDQKWALHRKIISPVFNMENLKLLIPVVEESVVDM 185

Query: 189 VEKWSAMADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASE 248
           VEKWSAMA   AS EVEI+VFD FQTL EDV+TKT FGSSYEDGKAIFKLQTQQMILASE
Sbjct: 186 VEKWSAMAGGAASDEVEIDVFDWFQTLAEDVVTKTVFGSSYEDGKAIFKLQTQQMILASE 245

Query: 249 AFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGL 308
           AFQKVFIPGYRFLPTRTNI+SWKLDKEI+KSLMKLIEGRKGNS+K WAMDEK PKDLLGL
Sbjct: 246 AFQKVFIPGYRFLPTRTNINSWKLDKEIRKSLMKLIEGRKGNSQKIWAMDEKAPKDLLGL 305

Query: 309 MIQASNSSSCSSLNNN----NITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQ 368
           MIQASNSSS SS +++    NI+VHDIVEECK+FFFAGKQTTSNLLTWTTVLLAMHPQWQ
Sbjct: 306 MIQASNSSSSSSSSSSSLHTNISVHDIVEECKTFFFAGKQTTSNLLTWTTVLLAMHPQWQ 365

Query: 369 IQARDEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKADVELGGRMI 428
           IQARDEVLSVCGPRALPSRDD+SK KILSMILNESLRLYPPTVATIRRAK  VEL GRMI
Sbjct: 366 IQARDEVLSVCGPRALPSRDDLSKFKILSMILNESLRLYPPTVATIRRAKTVVELRGRMI 425

Query: 429 PRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQN 488
           PRGTEFLIPILAVHHDQ+IWGNDASEFNPGRFSEGVG AGNHPAGFIPFG G+R CIG+N
Sbjct: 426 PRGTEFLIPILAVHHDQNIWGNDASEFNPGRFSEGVGHAGNHPAGFIPFGHGLRACIGKN 485

Query: 489 LALLQAKLAMAIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 533
           LALLQAKLA+AII+QRFSFRLAP+YQHAPTVLMLLYPQYGAPI+F+KI
Sbjct: 486 LALLQAKLAIAIIVQRFSFRLAPSYQHAPTVLMLLYPQYGAPIVFRKI 533

BLAST of Lag0034367 vs. NCBI nr
Match: XP_022933596.1 (cytochrome P450 734A1-like [Cucurbita moschata])

HSP 1 Score: 942.6 bits (2435), Expect = 1.5e-270
Identity = 463/524 (88.36%), Postives = 492/524 (93.89%), Query Frame = 0

Query: 9   PAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVS 68
           P PL+S+K+P LCA++ IL  KM  ALWWKPRRIE HFLRQGIRGPPYRFFIGNFKEL S
Sbjct: 6   PPPLDSIKIPFLCALLFILAVKMGAALWWKPRRIEAHFLRQGIRGPPYRFFIGNFKELDS 65

Query: 69  MMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREILTT 128
           MMLAA+S PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIR+ILTT
Sbjct: 66  MMLAATSRPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRDILTT 125

Query: 129 NSDFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDM 188
           NS+FYEKNEAHPL+K+LEGDGLLTLKDQKWALHRKIISP F+MENLKLLIPVVEESVVDM
Sbjct: 126 NSEFYEKNEAHPLIKKLEGDGLLTLKDQKWALHRKIISPVFNMENLKLLIPVVEESVVDM 185

Query: 189 VEKWSAMADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASE 248
           VEKWSAMA   AS EVEI+VFD FQTL EDV+TKT FGSSYEDGKAIFKLQTQQMILASE
Sbjct: 186 VEKWSAMAGGGASDEVEIDVFDWFQTLAEDVVTKTVFGSSYEDGKAIFKLQTQQMILASE 245

Query: 249 AFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGL 308
           AFQKVFIPGYRFLPTRTNI+SWKLDKEI+KSLMKLIEGRKGNS+K WAMDEK PKDLLGL
Sbjct: 246 AFQKVFIPGYRFLPTRTNINSWKLDKEIRKSLMKLIEGRKGNSQKIWAMDEKAPKDLLGL 305

Query: 309 MIQASNSSSCSSLNNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 368
           MIQASNSSS SS  + NI+VHDIVEECK+FFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR
Sbjct: 306 MIQASNSSSSSSSLHTNISVHDIVEECKTFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 365

Query: 369 DEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKADVELGGRMIPRGT 428
           DEVLSVCGPRALPS DD+SK KILSMILNESLRLYPPTVATIRRAK  VEL GRMIPRGT
Sbjct: 366 DEVLSVCGPRALPSTDDLSKFKILSMILNESLRLYPPTVATIRRAKTVVELRGRMIPRGT 425

Query: 429 EFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALL 488
           EFLIPILAVHHDQ+IWGNDASEFNPGRFSEGVG AGNHPAGFIPFG G+R CIG+NLALL
Sbjct: 426 EFLIPILAVHHDQNIWGNDASEFNPGRFSEGVGHAGNHPAGFIPFGHGLRACIGKNLALL 485

Query: 489 QAKLAMAIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 533
           QAKLA+AII+QRFSFRLAP+YQHAPTVLMLLYPQYGAP++F+KI
Sbjct: 486 QAKLAIAIIVQRFSFRLAPSYQHAPTVLMLLYPQYGAPVVFRKI 529

BLAST of Lag0034367 vs. NCBI nr
Match: KAG7021795.1 (Cytochrome P450, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 940.3 bits (2429), Expect = 7.6e-270
Identity = 463/527 (87.86%), Postives = 496/527 (94.12%), Query Frame = 0

Query: 9   PAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVS 68
           P PL+S+K+P LCA++ IL  KM VALWWKPRRIE HFLRQGIRGPPYRFFIGNFKEL S
Sbjct: 6   PPPLDSIKIPFLCALLFILAVKMGVALWWKPRRIEAHFLRQGIRGPPYRFFIGNFKELDS 65

Query: 69  MMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREILTT 128
           MMLAA+S PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIR+ILT 
Sbjct: 66  MMLAATSRPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRDILTN 125

Query: 129 NSDFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDM 188
           NS+FYEKNEAHPL+K+LEGDGLLTLKDQKWALHRKIISP F+MENLKLLIPVVEESVVDM
Sbjct: 126 NSEFYEKNEAHPLIKKLEGDGLLTLKDQKWALHRKIISPVFNMENLKLLIPVVEESVVDM 185

Query: 189 VEKWSAMADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASE 248
           VEKWSAMA   AS EVEI+VFD FQTL EDV+TKT FGSSYEDGKAIFKLQTQQMILASE
Sbjct: 186 VEKWSAMAGGGASDEVEIDVFDWFQTLAEDVVTKTVFGSSYEDGKAIFKLQTQQMILASE 245

Query: 249 AFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGL 308
           AFQKVFIPGYRFLPTRTNI+SWKLDKEI+KSLMKLIEGRKGNS+K WAMDEK PKDLLGL
Sbjct: 246 AFQKVFIPGYRFLPTRTNINSWKLDKEIRKSLMKLIEGRKGNSQKIWAMDEKAPKDLLGL 305

Query: 309 MIQASNSSSCSSLNNN---NITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQI 368
           MIQASNSSS SS +++   NI+VHDIVEECK+FFFAGKQTTSNLLTWTTVLLAMHPQWQI
Sbjct: 306 MIQASNSSSSSSSSSSLHTNISVHDIVEECKTFFFAGKQTTSNLLTWTTVLLAMHPQWQI 365

Query: 369 QARDEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKADVELGGRMIP 428
           QARDEVLS+CGPRALPSRDD+SK KILSMILNESLRLYPPTVATIRRAK  VEL GRMIP
Sbjct: 366 QARDEVLSLCGPRALPSRDDLSKFKILSMILNESLRLYPPTVATIRRAKTVVELRGRMIP 425

Query: 429 RGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNL 488
           RGTEFLIPILAVHHDQ+IWGNDASEFNPGRFSEGVG+AGNHPAGFIPFG G+R CIG+NL
Sbjct: 426 RGTEFLIPILAVHHDQNIWGNDASEFNPGRFSEGVGQAGNHPAGFIPFGHGLRACIGKNL 485

Query: 489 ALLQAKLAMAIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 533
           ALLQAKLA+AII+QRFSFRLAP+YQHAPTVLMLLYPQYGAP++F+KI
Sbjct: 486 ALLQAKLAIAIIVQRFSFRLAPSYQHAPTVLMLLYPQYGAPVVFRKI 532

BLAST of Lag0034367 vs. NCBI nr
Match: XP_038880221.1 (cytochrome P450 734A1-like [Benincasa hispida])

HSP 1 Score: 939.9 bits (2428), Expect = 9.9e-270
Identity = 471/530 (88.87%), Postives = 494/530 (93.21%), Query Frame = 0

Query: 12  LESV--KVP------VLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNF 71
           +ESV  KVP      V  AVV+IL AKM + LWWKPRRIE+HF RQGIRGPPYRFFIGNF
Sbjct: 3   IESVLSKVPPVWAAAVTAAVVMILSAKMVLVLWWKPRRIEKHFDRQGIRGPPYRFFIGNF 62

Query: 72  KELVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIR 131
           KE+VSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIR
Sbjct: 63  KEVVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIR 122

Query: 132 EILTTNSDFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEE 191
           EI TTNS FYEKNEAHPLVKQLEGDGLLTLK QKWALHRKIISPTFHM+NLKLLIP+VEE
Sbjct: 123 EIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLIPLVEE 182

Query: 192 SVVDMVEKWSAMADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQM 251
           SVV+MVEKWS+MA+ T S EVEI+VF+CFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQM
Sbjct: 183 SVVEMVEKWSSMAESTDSDEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQM 242

Query: 252 ILASEAFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPK 311
           ILAS+AF KVFIPGYRFLPTRTNI+SWKLDKEIKKSLMKLIEGRK NSEKNW MDEK PK
Sbjct: 243 ILASQAFHKVFIPGYRFLPTRTNINSWKLDKEIKKSLMKLIEGRKWNSEKNWGMDEKRPK 302

Query: 312 DLLGLMIQASNSSSCSSLNNNN-ITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQ 371
           DLLGLMIQAS+    ++ NNNN ITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQ
Sbjct: 303 DLLGLMIQASSLKYNNNNNNNNSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQ 362

Query: 372 WQIQARDEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKADVELGGR 431
           WQIQARDEV +VCG RALPS+ DVSK KILSMILNESLRLYPPTVATIRRAKADVELGGR
Sbjct: 363 WQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVELGGR 422

Query: 432 MIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIG 491
           MIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLG RTCIG
Sbjct: 423 MIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGARTCIG 482

Query: 492 QNLALLQAKLAMAIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 533
           QNLA+L+AKL MAIILQRF+FRLAPTYQHAPTVLMLLYPQYGAPI+FQKI
Sbjct: 483 QNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQKI 532

BLAST of Lag0034367 vs. ExPASy Swiss-Prot
Match: O48786 (Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 1.0e-216
Identity = 370/520 (71.15%), Postives = 435/520 (83.65%), Query Frame = 0

Query: 16  KVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASS 75
           KV VL  ++ ++  K    LWW+PR+IEEHF +QGIRGPPY FFIGN KELV MML ASS
Sbjct: 12  KVLVLSVILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASS 71

Query: 76  HPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREILTTNSDFYEK 135
           HPMP FSHNILPRVLSFYHHW+KIYG+TFL+WFGP  R+ V+DPD+IREI  + S+FYEK
Sbjct: 72  HPMP-FSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIF-SKSEFYEK 131

Query: 136 NEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSAM 195
           NEAHPLVKQLEGDGLL+LK +KWA HRKIISPTFHMENLKLL+PVV +SV DMV+KWS  
Sbjct: 132 NEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSDK 191

Query: 196 ADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVFI 255
             +  +GEVE++V++ FQ LTEDVI++TAFGSSYEDG+A+F+LQ QQM+L +EAFQKVFI
Sbjct: 192 LSE--NGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFI 251

Query: 256 PGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGP--KDLLGLMIQAS 315
           PGYRF PTR N+ SWKLDKEI+KSL+KLIE R+ N+      + K P  KDLLGLMIQA 
Sbjct: 252 PGYRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMIQA- 311

Query: 316 NSSSCSSLNNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLS 375
                      N+TV DIVEECKSFFFAGKQTTSNLLTWTT+LL+MHP+WQ +ARDEVL 
Sbjct: 312 ----------KNVTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLR 371

Query: 376 VCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKADVELGGRMIPRGTEFLIP 435
           VCG R +P++D V KLK LSMILNESLRLYPP VATIRRAK+DV+LGG  IP GTE LIP
Sbjct: 372 VCGSRDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIP 431

Query: 436 ILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLA 495
           I+AVHHDQ+IWGND +EFNP RF++GV RA  HP GFIPFGLGVRTCIGQNLA+LQAKL 
Sbjct: 432 IIAVHHDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLT 491

Query: 496 MAIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIS 534
           +A+++QRF+F LAPTYQHAPTVLMLLYPQ+GAPI F++++
Sbjct: 492 LAVMIQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRLT 516

BLAST of Lag0034367 vs. ExPASy Swiss-Prot
Match: B9X287 (Cytochrome P450 734A6 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A6 PE=2 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 2.5e-191
Identity = 327/515 (63.50%), Postives = 408/515 (79.22%), Query Frame = 0

Query: 22  AVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASSHPM--P 81
           A    +  K+   LWW+PRR+EEHF RQGI GP YRF +G  +E+V++M+AAS+ PM  P
Sbjct: 12  AAAAWVAVKVLEVLWWRPRRVEEHFARQGITGPRYRFLVGCVREMVALMVAASAKPMPPP 71

Query: 82  NFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREILTTNSDFYEKNEAH 141
             SHN+LPRVL+FYHHWKKIYGSTFLIWFGP  R+A++DP++IRE+L   +D +++ E+H
Sbjct: 72  YRSHNVLPRVLAFYHHWKKIYGSTFLIWFGPTPRLAIADPELIREVLLARADRFDRYESH 131

Query: 142 PLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSAMADDT 201
           P+V+QLEG+GL++L+  KWA HR++++P FHM+NL+LL+P V  +V+DM +KW AMA+  
Sbjct: 132 PMVRQLEGEGLVSLRGDKWAHHRRVLTPAFHMDNLRLLLPCVGMTVLDMADKWRAMAEAD 191

Query: 202 ASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVFIPGYR 261
            SGEVEI+V D FQ +TED IT+TAFG SYEDGK +FKLQ Q M  ASEAF+KVFIPGYR
Sbjct: 192 KSGEVEIDVSDWFQVVTEDAITRTAFGRSYEDGKVVFKLQAQLMAFASEAFRKVFIPGYR 251

Query: 262 FLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGLMIQA---SNSS 321
           FLPT+ N SSWKLDKEI+K+L+ LI GR+  +  +  +D    KDLLGLMI A   SN  
Sbjct: 252 FLPTKKNTSSWKLDKEIRKNLVTLI-GRRQEAGDDEKLDGCA-KDLLGLMINAAASSNGG 311

Query: 322 SCSSLNNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSVCG 381
             S+L  + ITV+DIVEECK+FFFAGKQTTSNLLTW  V+LAMHP+WQ +AR EVL VCG
Sbjct: 312 KRSALPVSPITVNDIVEECKTFFFAGKQTTSNLLTWAIVVLAMHPEWQERARQEVLDVCG 371

Query: 382 PRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKADVELGGRM-IPRGTEFLIPIL 441
              +PSR+ ++KLK L MILNE+LRLYPP VAT+RRAKADVELGG + IPR TE LIPI+
Sbjct: 372 ADGVPSREQLAKLKTLGMILNETLRLYPPAVATVRRAKADVELGGYLRIPRDTELLIPIM 431

Query: 442 AVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLAMA 501
           AVHHD  +WG DA++FNP RF+ GV RA  HPA FIPFGLG R CIGQNLA+L+AKL +A
Sbjct: 432 AVHHDARLWGPDAAQFNPARFAGGVARAARHPAAFIPFGLGARMCIGQNLAILEAKLTVA 491

Query: 502 IILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQ 531
           +IL RF FRL+  Y HAPTVLMLL+PQYGAPI+F+
Sbjct: 492 VILHRFEFRLSARYVHAPTVLMLLHPQYGAPIVFR 524

BLAST of Lag0034367 vs. ExPASy Swiss-Prot
Match: Q6Z6D6 (Cytochrome P450 734A2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A2 PE=2 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 7.8e-177
Identity = 300/527 (56.93%), Postives = 387/527 (73.43%), Query Frame = 0

Query: 23  VVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASSHPM-PNF 82
           V + + A+MA ALWW+PRR+E HF  QG+RGPPYRF +G+ +E+V++M  ASS PM P  
Sbjct: 29  VTMHVAARMADALWWRPRRLEAHFAAQGVRGPPYRFLLGSVREMVALMAEASSKPMSPPT 88

Query: 83  SHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREILTTNSDFYEKNEAHPL 142
           SHN LPRVL+FYH+W+KIYG  FLIWFGP  R+ V++P++IREI  T +D +++ EAHP+
Sbjct: 89  SHNALPRVLAFYHYWRKIYGHRFLIWFGPTPRLTVAEPELIREIFLTRADAFDRYEAHPV 148

Query: 143 VKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSAMADDTAS 202
           V+QLEGDGL++L   KWALHR++++  F+ +NL  LIP V +SV  +  KW AMA+   S
Sbjct: 149 VRQLEGDGLVSLHGDKWALHRRVLTDAFYPDNLNRLIPHVGKSVAALAAKWGAMAEAGGS 208

Query: 203 GEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVFIPGYRFL 262
           GEVE++V + FQ +TE+ IT+  FG SY+DG+ +F +Q Q M  ASEAF+KV +PGYRFL
Sbjct: 209 GEVEVDVAEWFQAVTEEAITRATFGRSYDDGRVVFAMQGQLMAFASEAFRKVLVPGYRFL 268

Query: 263 PTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGLMIQA-------SN 322
           PT+ N  SW+LD+EI++SLM+LI  R   +E+    D+   +DLLGLMI A        N
Sbjct: 269 PTKKNRLSWRLDREIRRSLMRLIGRRSDEAEQGEKADDGSFRDLLGLMINAGAAAATRGN 328

Query: 323 SSSCSSLNNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSV 382
           +    +     I V D++EECK+FFFAGKQTT+NLLTW TVLLAMHP WQ +AR EV  V
Sbjct: 329 AGGEKNSPAAAIPVEDMLEECKTFFFAGKQTTTNLLTWATVLLAMHPDWQERARREVFDV 388

Query: 383 CGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKADVELG-GRMIPRGTEFLIP 442
           CG   LPS++ + KLK L MI+NE+LRLYPP VATIRRAK DV+L  G MIPR  E L+P
Sbjct: 389 CGAGELPSKEHLPKLKTLGMIMNETLRLYPPAVATIRRAKVDVQLSDGCMIPRDMELLVP 448

Query: 443 ILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLA 502
           I+A+HHD   WG DAS+FNP RF+ G  +A  HP  FIPFGLG R C+GQNLA L+AKL 
Sbjct: 449 IMAIHHDTRYWGPDASQFNPARFANGASKAAKHPLAFIPFGLGSRMCVGQNLARLEAKLT 508

Query: 503 MAIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKISQFTGDAS 541
           MAI+LQRF  R +P Y HAPTVLMLLYPQYGAP+IF+ +S    D++
Sbjct: 509 MAILLQRFEIRTSPNYVHAPTVLMLLYPQYGAPLIFRPLSSHPPDST 555

BLAST of Lag0034367 vs. ExPASy Swiss-Prot
Match: Q69XM6 (Cytochrome P450 734A4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A4 PE=2 SV=1)

HSP 1 Score: 592.8 bits (1527), Expect = 3.9e-168
Identity = 293/538 (54.46%), Postives = 387/538 (71.93%), Query Frame = 0

Query: 12  LESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMML 71
           +E+V V     +++ + A++A A+WW+PRR+E HF  QG+RGPPYRF +G  +E+V++M 
Sbjct: 2   MEAVAVAAAVLLLLHVAARVADAVWWRPRRLEAHFAGQGVRGPPYRFLVGCVREMVALMA 61

Query: 72  AASSHPMPNFS-HNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREILTTNS 131
            A++ PMP  + HN LPRVL+FYH+W+KIYG TFLIWFGP  R+ V++P+M+REI  T +
Sbjct: 62  EATAKPMPPAAPHNALPRVLAFYHYWRKIYGPTFLIWFGPTPRLTVAEPEMVREIFLTRA 121

Query: 132 DFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVE 191
           + +++ EAHP+V+QLEGDGL++L   KWA HR++++P F+ +NL  L+P V  SV  + E
Sbjct: 122 EAFDRYEAHPVVRQLEGDGLVSLHGDKWAHHRRVLTPGFYPDNLNRLVPHVGRSVAALAE 181

Query: 192 KWSAMADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAF 251
           +W AMA     GEVE++V + FQ + E+ IT+  FG SY+ G+ +F+LQ + M  ASEAF
Sbjct: 182 RWRAMA-CAGGGEVEVDVAEWFQAVAEEAITRATFGRSYDSGRVVFRLQARLMAFASEAF 241

Query: 252 QKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKG-----NSEKNWAMDEK---GP 311
           +KV +PGYRFLPT+ N  SW LD+EI++ L++LI  R G       E    + +K   G 
Sbjct: 242 RKVLVPGYRFLPTKKNRMSWGLDREIRRGLVRLIGRRSGGDGGEEDETTTELKDKQDSGF 301

Query: 312 KDLLGLMIQASNSSSCSSLNNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQ 371
            DLLGLMI A          +  + V D+VEECK+FFFAGKQTT+NLLTW TVLLAMHP 
Sbjct: 302 NDLLGLMINAG--------VDRTMPVEDMVEECKTFFFAGKQTTTNLLTWATVLLAMHPD 361

Query: 372 WQIQARDEVLSVCGPRA--LPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKADVELG 431
           WQ +AR EVL+VCG  A  LP++D + KLK L MILNE+LRLYPP VATIRRAK DV LG
Sbjct: 362 WQDRARREVLAVCGDAAGELPTKDHLPKLKTLGMILNETLRLYPPAVATIRRAKFDVTLG 421

Query: 432 GR--------MIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIP 491
           G          IPR TE L+PI+A+HHD  +WG DA++FNP RF+ G  RA  HP  FIP
Sbjct: 422 GGGDGDAGGIHIPRDTELLVPIMAIHHDARLWGPDAAQFNPARFASGAARAAKHPLAFIP 481

Query: 492 FGLGVRTCIGQNLALLQAKLAMAIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQ 531
           FGLG R CIGQ+LA+L+AKL MA++LQRF   L+PTY HAPTVLMLL+PQYGAP+IF+
Sbjct: 482 FGLGSRMCIGQSLAILEAKLTMAVLLQRFDLALSPTYVHAPTVLMLLHPQYGAPLIFR 530

BLAST of Lag0034367 vs. ExPASy Swiss-Prot
Match: Q8LIF2 (Cytochrome P450 734A5 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A5 PE=2 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 5.6e-151
Identity = 262/519 (50.48%), Postives = 361/519 (69.56%), Query Frame = 0

Query: 24  VVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASSHPM-PNFS 83
           V    A+ A ALWW+PRR+E HF  QG+RGP YRFF+G+  ELV +M+ A+S PM P  S
Sbjct: 28  VYAAAARAAEALWWRPRRVERHFAAQGVRGPGYRFFVGSSIELVRLMVDAASRPMEPPTS 87

Query: 84  HNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREILTTNSDFYEKNEAHPLV 143
           H+ILPRVL FYHHW+K+YG   LIWFG   R+ VS+P++IRE+L T +D +++ EAHP++
Sbjct: 88  HDILPRVLPFYHHWRKLYGPMHLIWFGRTPRLVVSEPELIREVLLTRADHFDRYEAHPMI 147

Query: 144 KQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSAMADDTASG 203
            Q EG GL  L  ++WA  R++++P FH ENL+++ P V  +V  M+++ +  A    +G
Sbjct: 148 CQFEGYGLSNLHGERWARRRRVLTPAFHTENLRMIAPFVAGTVTRMLDELAERARAGGAG 207

Query: 204 EVEIEVFDCFQTLTEDVITKTAFG-SSYEDGKAIFKLQTQQMILASEAFQKVFIPGYRFL 263
           E E++V + FQ + ++ IT  AFG  +Y+DG A+F+LQ +    A+EA  KV+IPGYRFL
Sbjct: 208 EAEVDVAEWFQRVPQEAITFAAFGRRNYDDGAAVFRLQDELAGYATEAHSKVYIPGYRFL 267

Query: 264 PTRTNISSWKLDKEIKKSLMKLIEGRK------GNSEKNWAMDEKGPKDLLGLMIQASNS 323
           PTR N   W+LD+EI+  L K + G +      G+   +      G ++ +  M  A   
Sbjct: 268 PTRKNRRVWQLDREIRSHLAKFVTGLQSCSSSHGDDADDGGDGGGGMREFMSFMAPA--- 327

Query: 324 SSCSSLNNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSVC 383
                     +T  +I+EE K+FFFAGK+T SNLLTWTTV LAMHP+WQ +AR EV++VC
Sbjct: 328 ----------MTAGEIIEESKNFFFAGKETLSNLLTWTTVALAMHPEWQERARREVVAVC 387

Query: 384 GPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKADVELGGRMIPRGTEFLIPIL 443
           G   LP++D + KLK L MILNE+LRLYPP VA IR AK DVELGG ++P GTE +IPI+
Sbjct: 388 GRGDLPTKDHLPKLKTLGMILNETLRLYPPAVAMIRTAKEDVELGGCVVPAGTEVMIPIM 447

Query: 444 AVHHDQSIWGNDASEFNPGRF-SEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLAM 503
           AVHHD + WG+DA+EFNP RF ++  G    HP  F+PFG G R CIGQN+AL++AK+A+
Sbjct: 448 AVHHDAAAWGDDAAEFNPARFAADDDGGRRRHPMAFMPFGGGARVCIGQNMALMEAKVAL 507

Query: 504 AIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIS 534
           A++L+RF FRL+P Y HAP VLM+L PQ+GAP+IF+ ++
Sbjct: 508 AVVLRRFEFRLSPAYVHAPRVLMILSPQFGAPVIFRPLT 533

BLAST of Lag0034367 vs. ExPASy TrEMBL
Match: A0A6J1L528 (cytochrome P450 734A1-like OS=Cucurbita maxima OX=3661 GN=LOC111499208 PE=3 SV=1)

HSP 1 Score: 946.4 bits (2445), Expect = 5.1e-272
Identity = 466/530 (87.92%), Postives = 494/530 (93.21%), Query Frame = 0

Query: 9   PAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVS 68
           P P +S+K+P LCA++ IL  KMAVALWWKPRRIE HFLRQGIRGPPYRFFIGNFKEL S
Sbjct: 6   PPPFDSIKIPFLCALLFILAVKMAVALWWKPRRIEAHFLRQGIRGPPYRFFIGNFKELDS 65

Query: 69  MMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREILTT 128
           MMLAA+S PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIR+ILTT
Sbjct: 66  MMLAATSRPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRDILTT 125

Query: 129 NSDFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDM 188
           NS+FYEKNEAHPL+K+LEGDGLLTLKDQKWALHRKIISP F+MENLKLLIPVVEESVVDM
Sbjct: 126 NSEFYEKNEAHPLIKKLEGDGLLTLKDQKWALHRKIISPVFNMENLKLLIPVVEESVVDM 185

Query: 189 VEKWSAMADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASE 248
           VEKWSAMA   AS EVEI+VFD FQTL EDV+TKT FGSSYEDGKAIFKLQTQQMILASE
Sbjct: 186 VEKWSAMAGGAASDEVEIDVFDWFQTLAEDVVTKTVFGSSYEDGKAIFKLQTQQMILASE 245

Query: 249 AFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGL 308
           AFQKVFIPGYRFLPTRTNI+SWKLDKEI+KSLMKLIE RKGNSEK WAMDEK PKDLLGL
Sbjct: 246 AFQKVFIPGYRFLPTRTNINSWKLDKEIRKSLMKLIESRKGNSEKIWAMDEKAPKDLLGL 305

Query: 309 MIQASNSSSCSSLNNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 368
           MIQASNSSS SS  + NI+VHDIVEECK+FFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR
Sbjct: 306 MIQASNSSSSSSSLHTNISVHDIVEECKTFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 365

Query: 369 DEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKADVELGGRMIPRGT 428
           DEVLSVCGPRALPSRDD+SK KILSMILNESLRLYPPTVATIRRAK  VEL GRMIPRGT
Sbjct: 366 DEVLSVCGPRALPSRDDLSKFKILSMILNESLRLYPPTVATIRRAKTVVELRGRMIPRGT 425

Query: 429 EFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALL 488
           EFLIPILAVHHDQ+IWGNDASEFNPGRFSEGVG AGNHPAGFIPFG G+R CIG+NLALL
Sbjct: 426 EFLIPILAVHHDQNIWGNDASEFNPGRFSEGVGHAGNHPAGFIPFGHGLRACIGKNLALL 485

Query: 489 QAKLAMAIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKISQFTGD 539
           QAKLA+AII+QRFSFRLAP+YQHAP VLMLLYPQYGAP++FQKI +   D
Sbjct: 486 QAKLAIAIIVQRFSFRLAPSYQHAPAVLMLLYPQYGAPVVFQKIVETRRD 535

BLAST of Lag0034367 vs. ExPASy TrEMBL
Match: A0A6J1EZI1 (cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111440975 PE=3 SV=1)

HSP 1 Score: 942.6 bits (2435), Expect = 7.4e-271
Identity = 463/524 (88.36%), Postives = 492/524 (93.89%), Query Frame = 0

Query: 9   PAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVS 68
           P PL+S+K+P LCA++ IL  KM  ALWWKPRRIE HFLRQGIRGPPYRFFIGNFKEL S
Sbjct: 6   PPPLDSIKIPFLCALLFILAVKMGAALWWKPRRIEAHFLRQGIRGPPYRFFIGNFKELDS 65

Query: 69  MMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREILTT 128
           MMLAA+S PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIR+ILTT
Sbjct: 66  MMLAATSRPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRDILTT 125

Query: 129 NSDFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDM 188
           NS+FYEKNEAHPL+K+LEGDGLLTLKDQKWALHRKIISP F+MENLKLLIPVVEESVVDM
Sbjct: 126 NSEFYEKNEAHPLIKKLEGDGLLTLKDQKWALHRKIISPVFNMENLKLLIPVVEESVVDM 185

Query: 189 VEKWSAMADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASE 248
           VEKWSAMA   AS EVEI+VFD FQTL EDV+TKT FGSSYEDGKAIFKLQTQQMILASE
Sbjct: 186 VEKWSAMAGGGASDEVEIDVFDWFQTLAEDVVTKTVFGSSYEDGKAIFKLQTQQMILASE 245

Query: 249 AFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGL 308
           AFQKVFIPGYRFLPTRTNI+SWKLDKEI+KSLMKLIEGRKGNS+K WAMDEK PKDLLGL
Sbjct: 246 AFQKVFIPGYRFLPTRTNINSWKLDKEIRKSLMKLIEGRKGNSQKIWAMDEKAPKDLLGL 305

Query: 309 MIQASNSSSCSSLNNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 368
           MIQASNSSS SS  + NI+VHDIVEECK+FFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR
Sbjct: 306 MIQASNSSSSSSSLHTNISVHDIVEECKTFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 365

Query: 369 DEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKADVELGGRMIPRGT 428
           DEVLSVCGPRALPS DD+SK KILSMILNESLRLYPPTVATIRRAK  VEL GRMIPRGT
Sbjct: 366 DEVLSVCGPRALPSTDDLSKFKILSMILNESLRLYPPTVATIRRAKTVVELRGRMIPRGT 425

Query: 429 EFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALL 488
           EFLIPILAVHHDQ+IWGNDASEFNPGRFSEGVG AGNHPAGFIPFG G+R CIG+NLALL
Sbjct: 426 EFLIPILAVHHDQNIWGNDASEFNPGRFSEGVGHAGNHPAGFIPFGHGLRACIGKNLALL 485

Query: 489 QAKLAMAIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 533
           QAKLA+AII+QRFSFRLAP+YQHAPTVLMLLYPQYGAP++F+KI
Sbjct: 486 QAKLAIAIIVQRFSFRLAPSYQHAPTVLMLLYPQYGAPVVFRKI 529

BLAST of Lag0034367 vs. ExPASy TrEMBL
Match: A0A1S3CMW0 (cytochrome P450 734A1-like OS=Cucumis melo OX=3656 GN=LOC103502730 PE=3 SV=1)

HSP 1 Score: 907.9 bits (2345), Expect = 2.0e-260
Identity = 451/537 (83.99%), Postives = 490/537 (91.25%), Query Frame = 0

Query: 1   MKENPAAAPAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFI 60
           MKE    +PA   +  V     ++ I+ AK+ + LWWKP++IE+HF RQGI+GPPYRFFI
Sbjct: 1   MKEVKIESPA-WSAAAVTTAVLLIFIISAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFI 60

Query: 61  GNFKELVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPD 120
           GNFKELVS+MLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPD
Sbjct: 61  GNFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPD 120

Query: 121 MIREILTTNSDFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPV 180
           MIREI  TNS +YEKNEAHPLVKQLEGDGLLTL  QKWALHRKIISPTFH++NLKLLIP+
Sbjct: 121 MIREIFATNSQYYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPL 180

Query: 181 VEESVVDMVEKWSAMADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQT 240
           VEESVV+MVEKW +MA+   S EVEI+V+D FQTLTEDVITKTAFGSSYEDGKAIFKLQ 
Sbjct: 181 VEESVVEMVEKWWSMAESVESSEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQN 240

Query: 241 QQMILASEAFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSE-KNWAMDE 300
           QQMILAS+AFQKVFIPGYRFLPTRTNI+SWKLDKEIKKSLMKLIEGRKGN E KNW M+E
Sbjct: 241 QQMILASQAFQKVFIPGYRFLPTRTNINSWKLDKEIKKSLMKLIEGRKGNLEKKNWGMNE 300

Query: 301 KGPKDLLGLMIQAS----NSSSCSSLNNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTV 360
           K PKDLLGLMIQAS    N+++ ++ NN++ITVHDIVEECKSFFFAGKQTTSNLLTWTTV
Sbjct: 301 KRPKDLLGLMIQASLLNNNNNNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTV 360

Query: 361 LLAMHPQWQIQARDEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKA 420
           LLAMHPQWQIQARDEV++VCGPRALPS+ DVS  KILSMILNESLRLYPPTVATIRRAKA
Sbjct: 361 LLAMHPQWQIQARDEVINVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKA 420

Query: 421 DVELGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGL 480
           DVEL GRMIPRGTEFLIPI+AVHHDQ+IWGNDASEFNPGRFSEGV RAGNHPAGFIPFGL
Sbjct: 421 DVELAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSRAGNHPAGFIPFGL 480

Query: 481 GVRTCIGQNLALLQAKLAMAIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 533
           GVRTCIGQNLA+L+AKL MAIILQRF+FRLAPTYQHAPTVLMLLYPQYGAPIIFQKI
Sbjct: 481 GVRTCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 536

BLAST of Lag0034367 vs. ExPASy TrEMBL
Match: A0A0A0LU20 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G305710 PE=3 SV=1)

HSP 1 Score: 901.0 bits (2327), Expect = 2.5e-258
Identity = 449/533 (84.24%), Postives = 489/533 (91.74%), Query Frame = 0

Query: 1   MKENPAAAPAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFI 60
           MKE    +PA   +V V ++  ++ I+ AK+ + LWWKP++IE+HF RQGI+GPPYRFFI
Sbjct: 1   MKEVKIESPAWSATV-VTIVVLLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFI 60

Query: 61  GNFKELVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPD 120
           GNFKELVS+MLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPD
Sbjct: 61  GNFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPD 120

Query: 121 MIREILTTNSDFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPV 180
           MIREI  TNS FYEKNEAHPLVKQLEGDGLLTL  QKWALHRKIISPTFH++NLKLLIP+
Sbjct: 121 MIREIFATNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPL 180

Query: 181 VEESVVDMVEKWSAMADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQT 240
           VEESVV+MVEKW +MA+   SGEVEI+V+D FQTLTEDVITKTAFGSSYEDGKAIFKLQT
Sbjct: 181 VEESVVEMVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQT 240

Query: 241 QQMILASEAFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSE-KNWAMDE 300
           QQMILAS+AFQKVFIPGYRFLPTRTNI+SWKLD+EIKKSLMKLIEGRK N E KNW M+E
Sbjct: 241 QQMILASQAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRNLEKKNWGMNE 300

Query: 301 KGPKDLLGLMIQASNSSSCSSLNNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAM 360
           K PKDLLGLMIQAS  ++ ++ NN++ITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAM
Sbjct: 301 KRPKDLLGLMIQASLLNN-NNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAM 360

Query: 361 HPQWQIQARDEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKADVEL 420
           HPQWQIQARDEV +VCGPRALPS+ DVS  KILSMILNESLRLYPPTVATIRRAK DVEL
Sbjct: 361 HPQWQIQARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVEL 420

Query: 421 GGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRT 480
            GRMIPRGTEFLIPI+AVHHDQ+IWGNDASEFNPGRFSEGV +A NHPAGFIPFGLGVRT
Sbjct: 421 AGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLGVRT 480

Query: 481 CIGQNLALLQAKLAMAIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 533
           CIGQNLALL+AKL +AIILQRF+FRLAPTYQHAPTVLMLLYPQYGAPIIFQKI
Sbjct: 481 CIGQNLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 531

BLAST of Lag0034367 vs. ExPASy TrEMBL
Match: A0A6J1H9G1 (cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111461725 PE=3 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.5e-255
Identity = 439/517 (84.91%), Postives = 467/517 (90.33%), Query Frame = 0

Query: 17  VPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASSH 76
           V     VVVIL AKM + LWWKPRRIE HFLRQGI GPPYRFFIGNFKELVSMM AASSH
Sbjct: 15  VAAAAVVVVILGAKMGLVLWWKPRRIENHFLRQGIGGPPYRFFIGNFKELVSMMAAASSH 74

Query: 77  PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREILTTNSDFYEKN 136
           P+PNFSHNILPRVLSFYHHWKKIYGSTF+IWFGPI RVAVSDPDMIREI  TNS FYEKN
Sbjct: 75  PIPNFSHNILPRVLSFYHHWKKIYGSTFVIWFGPIARVAVSDPDMIREIFITNSHFYEKN 134

Query: 137 EAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSAMA 196
           EAHPLVKQLEGDGLLTL   KWALHRKIISPTFH +NLKLLIP+VEESV+DMV+KWS+M 
Sbjct: 135 EAHPLVKQLEGDGLLTLNGPKWALHRKIISPTFHTDNLKLLIPLVEESVMDMVQKWSSMT 194

Query: 197 DDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVFIP 256
           ++T SGEVEI+VFD FQTLTEDVITKTAFG SYEDGKAIFK+QTQQMILASEAFQKVFIP
Sbjct: 195 EETTSGEVEIDVFDSFQTLTEDVITKTAFGCSYEDGKAIFKIQTQQMILASEAFQKVFIP 254

Query: 257 GYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGLMIQASNSS 316
           GYRFLPTRTN+ SWKL+KEIKKSL+KLIEGRK N EKNWA++EKGPKDLLGLMIQAS   
Sbjct: 255 GYRFLPTRTNMKSWKLEKEIKKSLLKLIEGRKRNFEKNWAINEKGPKDLLGLMIQAS--- 314

Query: 317 SCSSLNNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSVCG 376
                    I+VHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHP+WQI AR EV ++CG
Sbjct: 315 ---------ISVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPRWQILARHEVFTICG 374

Query: 377 PRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKADVELGGRMIPRGTEFLIPILA 436
           PRALPS DDVSK KILSMILNESLRLYPPTVATIRRA ADVELGGRMIPRGTEFLIPILA
Sbjct: 375 PRALPSSDDVSKFKILSMILNESLRLYPPTVATIRRANADVELGGRMIPRGTEFLIPILA 434

Query: 437 VHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLAMAI 496
           VHHDQ+IWGNDASEFNP RFS+GV RAGNHP GFIPFGLGVR CIGQNLA+L+AKLAM I
Sbjct: 435 VHHDQTIWGNDASEFNPERFSDGVSRAGNHPVGFIPFGLGVRNCIGQNLAVLEAKLAMVI 494

Query: 497 ILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIS 534
           ILQR SFRLAPTYQHAPTVLMLLYPQYGAPI+F+KI+
Sbjct: 495 ILQRLSFRLAPTYQHAPTVLMLLYPQYGAPILFEKIA 519

BLAST of Lag0034367 vs. TAIR 10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 754.2 bits (1946), Expect = 7.2e-218
Identity = 370/520 (71.15%), Postives = 435/520 (83.65%), Query Frame = 0

Query: 16  KVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASS 75
           KV VL  ++ ++  K    LWW+PR+IEEHF +QGIRGPPY FFIGN KELV MML ASS
Sbjct: 12  KVLVLSVILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASS 71

Query: 76  HPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREILTTNSDFYEK 135
           HPMP FSHNILPRVLSFYHHW+KIYG+TFL+WFGP  R+ V+DPD+IREI  + S+FYEK
Sbjct: 72  HPMP-FSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIF-SKSEFYEK 131

Query: 136 NEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSAM 195
           NEAHPLVKQLEGDGLL+LK +KWA HRKIISPTFHMENLKLL+PVV +SV DMV+KWS  
Sbjct: 132 NEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSDK 191

Query: 196 ADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVFI 255
             +  +GEVE++V++ FQ LTEDVI++TAFGSSYEDG+A+F+LQ QQM+L +EAFQKVFI
Sbjct: 192 LSE--NGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFI 251

Query: 256 PGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGP--KDLLGLMIQAS 315
           PGYRF PTR N+ SWKLDKEI+KSL+KLIE R+ N+      + K P  KDLLGLMIQA 
Sbjct: 252 PGYRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMIQA- 311

Query: 316 NSSSCSSLNNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLS 375
                      N+TV DIVEECKSFFFAGKQTTSNLLTWTT+LL+MHP+WQ +ARDEVL 
Sbjct: 312 ----------KNVTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLR 371

Query: 376 VCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKADVELGGRMIPRGTEFLIP 435
           VCG R +P++D V KLK LSMILNESLRLYPP VATIRRAK+DV+LGG  IP GTE LIP
Sbjct: 372 VCGSRDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIP 431

Query: 436 ILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLA 495
           I+AVHHDQ+IWGND +EFNP RF++GV RA  HP GFIPFGLGVRTCIGQNLA+LQAKL 
Sbjct: 432 IIAVHHDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLT 491

Query: 496 MAIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIS 534
           +A+++QRF+F LAPTYQHAPTVLMLLYPQ+GAPI F++++
Sbjct: 492 LAVMIQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRLT 516

BLAST of Lag0034367 vs. TAIR 10
Match: AT3G14620.1 (cytochrome P450, family 72, subfamily A, polypeptide 8 )

HSP 1 Score: 409.8 bits (1052), Expect = 3.3e-114
Identity = 213/522 (40.80%), Postives = 323/522 (61.88%), Query Frame = 0

Query: 17  VPVLCAVVVILCAKMAV-----ALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMML 76
           V V  AVVV+    + +       W +P++ E +  RQG+ G P+ F +G+ K   SM+ 
Sbjct: 9   VAVAAAVVVVTTVTVWIWKGLNVAWLRPKKNEAYLKRQGLSGTPFTFLVGDIKREASMVE 68

Query: 77  AASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREILTTNSD 136
              S P+ N + +   RV+       K +G T  +W GPI  V V+ P+ I+++L    D
Sbjct: 69  QEKSRPI-NLTDDYTHRVMPLIQQTVKDHGKTSYMWMGPIASVIVTKPEHIKDVLNRVYD 128

Query: 137 FYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEK 196
           F  K   HP+V +L   G+   + +KW+ HRKII+P+FH+E LK++IP   ES  +M+ K
Sbjct: 129 F-PKPPVHPIV-ELFATGVALYEGEKWSKHRKIINPSFHLEKLKIMIPAFYESCSEMISK 188

Query: 197 WSAMADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQ 256
           W  +  +  S   EI+V+     LT DVI++TAFGSSYE+GK IF+LQ +Q     +A +
Sbjct: 189 WEKLVTEQGSSN-EIDVWPYLGDLTSDVISRTAFGSSYEEGKRIFELQEEQGRRVLKALE 248

Query: 257 KVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGLMIQ 316
             FIPG RFLPT+ N+   +++KE+K  L ++I  R    ++     E    DLLG++++
Sbjct: 249 LAFIPGMRFLPTKNNLRMRQINKEVKSRLREIIMKR----QRGMDTGEAPKNDLLGILLE 308

Query: 317 ASNSSSCSSLNNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEV 376
                  S+  ++ +++ D+VEEC+ F FAG++TT+ LL WT ++L+ H +WQ QAR+E+
Sbjct: 309 -------SNSGDHGMSIEDVVEECRLFHFAGQETTAVLLVWTMIMLSHHQKWQDQAREEI 368

Query: 377 LSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKADVELGGRM-IPRGTEF 436
           L V G    P+ D +S+LK +SMILNE LRLYPP +   R  + + +LG  M +P G + 
Sbjct: 369 LKVIGKNNKPNFDALSRLKTMSMILNEVLRLYPPGILLGRTVEKETKLGEDMTLPGGAQV 428

Query: 437 LIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQA 496
           +IP+L VH D  +WG D  EFNP RF++G+ +A  +   F+PFG G R C GQN AL++A
Sbjct: 429 VIPVLMVHRDPELWGEDVHEFNPERFADGISKATKNQVSFLPFGWGPRFCPGQNFALMEA 488

Query: 497 KLAMAIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 533
           K+A+ +ILQRFSF L+P+Y HAP  ++ L+PQ+GAP+IF  +
Sbjct: 489 KMALVLILQRFSFELSPSYTHAPHTVLTLHPQFGAPLIFHML 515

BLAST of Lag0034367 vs. TAIR 10
Match: AT3G14610.1 (cytochrome P450, family 72, subfamily A, polypeptide 7 )

HSP 1 Score: 404.8 bits (1039), Expect = 1.1e-112
Identity = 208/516 (40.31%), Postives = 318/516 (61.63%), Query Frame = 0

Query: 17  VPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASSH 76
           +PVL AVVV+   ++   +W KP+ +E    RQG+ G PY   +G+ K  V MM+ A S 
Sbjct: 9   LPVLVAVVVLWTWRIVKWVWIKPKMLESSLKRQGLTGTPYTPLVGDIKRNVDMMMEARSK 68

Query: 77  PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREILTTNSDFYEKN 136
           P+ N + +I PR+L         +G TF IW GP+  + +++P+ I+E+    +DF EK 
Sbjct: 69  PI-NVTDDITPRLLPLALKMLNSHGKTFFIWIGPLPTIVITNPEQIKEVFNKVNDF-EKA 128

Query: 137 EAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSAMA 196
              PL++ L G GL + K  KWA HR+II+P FH+E +K +IP       ++V +W  + 
Sbjct: 129 STFPLIRLLAG-GLASYKGDKWASHRRIINPAFHLEKIKNMIPAFYHCCSEVVCQWEKLF 188

Query: 197 DDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVFIP 256
            D  S  +E++V+     +T DVI+ TAFGSSY++G+ IF+LQ +   L ++AF+K +IP
Sbjct: 189 TDKES-PLEVDVWPWLVNMTADVISHTAFGSSYKEGQRIFQLQGELAELIAQAFKKSYIP 248

Query: 257 GYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGLMIQASNSS 316
           G RF PT++N     +D+E+   L  ++  R    EK     E    DLLG+++++++  
Sbjct: 249 GSRFYPTKSNRRMKAIDREVDVILRGIVSKR----EKAREAGEPANDDLLGILLESNSEE 308

Query: 317 SCSSLNNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSVCG 376
           S      N ++V D+++ECK F+FAG++TTS LL WT VLL+ H  WQ +AR+EV+ V G
Sbjct: 309 S----QGNGMSVEDVMKECKLFYFAGQETTSVLLVWTMVLLSHHQDWQARAREEVMQVLG 368

Query: 377 PRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKADVELGGRMIPRGTEFLIPILA 436
               P  + ++ LK+++MI NE LRLYPP     R    +++LG   +P G +  +P + 
Sbjct: 369 ENNKPDMESLNNLKVMTMIFNEVLRLYPPVAQLKRVVNKEMKLGELTLPAGIQIYLPTIL 428

Query: 437 VHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLAMAI 496
           V  D  +WG+DA++F P RF +G+ +A  +   F PFG G R CIGQN A+L+AK+AMA+
Sbjct: 429 VQRDTELWGDDAADFKPERFRDGLSKATKNQVSFFPFGWGPRICIGQNFAMLEAKMAMAL 488

Query: 497 ILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 533
           ILQ+FSF L+P+Y HAP  +M   PQ+GA +I  K+
Sbjct: 489 ILQKFSFELSPSYVHAPQTVMTTRPQFGAHLILHKL 512

BLAST of Lag0034367 vs. TAIR 10
Match: AT3G14680.1 (cytochrome P450, family 72, subfamily A, polypeptide 14 )

HSP 1 Score: 401.0 bits (1029), Expect = 1.5e-111
Identity = 208/498 (41.77%), Postives = 306/498 (61.45%), Query Frame = 0

Query: 35  LWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASSHPMPNFSHNILPRVLSFYH 94
           +W+ P+ +E    RQG+ G  Y   IG+FK+++SM + A+S P+   + +I PRV+    
Sbjct: 28  VWFTPKMLERSLRRQGLSGTSYTPLIGDFKKMISMFIEATSKPIKP-TDDITPRVMPHPL 87

Query: 95  HWKKIYGSTFLIWFGPIVRVAVSDPDMIREILTTNSDFYEKNEAHPLVKQLEGDGLLTLK 154
              K +G T L WFGPI  + + DP+ I+E+     DF +K    PL K L G GL++  
Sbjct: 88  QMLKTHGRTNLTWFGPIPTITIMDPEQIKEVFNKVYDF-QKAHTFPLSKIL-GTGLVSYD 147

Query: 155 DQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSAMADDTASGEVEIEVFDCFQT 214
             KWA HR+II+P FH+E +K ++ V  ES  ++V +W  +  D  S   E++V+    +
Sbjct: 148 GDKWAQHRRIINPAFHLEKIKNMVHVFHESCSELVGEWDKLVSDKGS-SCEVDVWPGLTS 207

Query: 215 LTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVFIPGYRFLPTRTNISSWKLDK 274
           +T DVI++TAFGSSY +G  IF+LQ +   L  +AFQK FIPGY +LPT+ N       +
Sbjct: 208 MTADVISRTAFGSSYREGHRIFELQAELAQLVMQAFQKFFIPGYIYLPTKGNRRMKTAAR 267

Query: 275 EIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGLMIQASNSSSCSSLNNNNITVHDIVEE 334
           EI+  L  +I  R+   E   A  E    DLLG++++    S+      N ++  D++EE
Sbjct: 268 EIQDILRGIINKRERARESGEAPSE----DLLGILLE----SNLGQTEGNGMSTEDMMEE 327

Query: 335 CKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSVCGPRALPSRDDVSKLKILSM 394
           CK F+ AG++TTS LL WT VLL+ H  WQ +AR+EV  V G +  P  + +++LK+++M
Sbjct: 328 CKLFYLAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKQ-PDTEGLNQLKVMTM 387

Query: 395 ILNESLRLYPPTVATIRRAKADVELGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPG 454
           IL E LRLYPP V   R    +++LG   +P G +  +P+L VH D  +WGNDA EF P 
Sbjct: 388 ILYEVLRLYPPVVQLTRAIHKEMKLGDLTLPGGVQISLPVLLVHRDTELWGNDAGEFKPE 447

Query: 455 RFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLAMAIILQRFSFRLAPTYQHAPT 514
           RF +G+ +A  +   F PF  G R CIGQN  LL+AK+AM++ILQRFSF L+P+Y HAP 
Sbjct: 448 RFKDGLSKATKNQVSFFPFAWGPRICIGQNFTLLEAKMAMSLILQRFSFELSPSYVHAPY 507

Query: 515 VLMLLYPQYGAPIIFQKI 533
            ++ LYPQ+GA ++  K+
Sbjct: 508 TIITLYPQFGAHLMLHKL 512

BLAST of Lag0034367 vs. TAIR 10
Match: AT1G75130.1 (cytochrome P450, family 721, subfamily A, polypeptide 1 )

HSP 1 Score: 396.4 bits (1017), Expect = 3.8e-110
Identity = 213/518 (41.12%), Postives = 310/518 (59.85%), Query Frame = 0

Query: 19  VLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASSHPM 78
           +L  V   L  +   +  W P RI+ HF +Q + GP YR F GN  E+  +   A S P+
Sbjct: 6   ILVLVFFFLVFRFIYSNIWVPWRIQSHFKKQSVTGPSYRIFSGNSGEVSRLTAEAKSKPI 65

Query: 79  PN--FSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREILTTNSDFYEKN 138
           P+    H  + RV   YH W ++YG TFL WFG    VA SDP +IRE LTT   F ++ 
Sbjct: 66  PSGRNPHEFVHRVAPHYHEWSRVYGKTFLYWFGSKPVVATSDPRLIREALTTGGSF-DRI 125

Query: 139 EAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSAMA 198
             +PL K L   GL  L+  +WA HR+I    F ME LK  +P +  S + ++EKW  M 
Sbjct: 126 GHNPLSKLLYAQGLPGLRGDQWAFHRRIAKQAFTMEKLKRWVPQMVTSTMMLMEKWEDMR 185

Query: 199 DDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVFIP 258
           +     E+E+EV      L+ +++++TAFG+S E+GK IF+LQ + M L       V+IP
Sbjct: 186 N--GGEEIELEVHKEMHNLSAEMLSRTAFGNSVEEGKGIFELQERMMRLFYLVRWSVYIP 245

Query: 259 GYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGLMIQASNSS 318
           G+RF P++TN   W+++K+I+ S++KLIE  K   EK+            G ++QA  S 
Sbjct: 246 GFRFFPSKTNREIWRIEKQIRVSILKLIENNKTAVEKS------------GTLLQAFMSP 305

Query: 319 -SCSSLNNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSVC 378
            +  +     + + ++ +ECK+F+FA K+TT+NL+T+  VLLAM+ +WQ  AR+EV+ V 
Sbjct: 306 YTNQNGQEEKLGIEEVTDECKTFYFAAKETTANLMTFVLVLLAMNQEWQNIAREEVICVL 365

Query: 379 GPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKADVELGGRMIPRGTEFLIPIL 438
           G   LP+ D +  LK LSMI+NE+LRLYPP +   R      +LG   IP GT+  + ++
Sbjct: 366 GQTGLPTLDILQDLKTLSMIINETLRLYPPAMTLNRDTLKRAKLGDLDIPAGTQLYLSVV 425

Query: 439 AVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLAMA 498
           A+HHD+  WG+DA EFNP RF +         A  +PFGLG RTC+GQNLA+ +AK  +A
Sbjct: 426 AMHHDKETWGDDAEEFNPRRFED----PKKQSALLVPFGLGPRTCVGQNLAVNEAKTVLA 485

Query: 499 IILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIS 534
            IL+ +SFRL+P+Y HAP + + L PQ GA ++F +IS
Sbjct: 486 TILKYYSFRLSPSYAHAPVLFVTLQPQNGAHLLFTRIS 504

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023006493.11.1e-27187.92cytochrome P450 734A1-like [Cucurbita maxima][more]
XP_023531517.12.4e-27188.45cytochrome P450 734A1-like [Cucurbita pepo subsp. pepo][more]
XP_022933596.11.5e-27088.36cytochrome P450 734A1-like [Cucurbita moschata][more]
KAG7021795.17.6e-27087.86Cytochrome P450, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_038880221.19.9e-27088.87cytochrome P450 734A1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
O487861.0e-21671.15Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1[more]
B9X2872.5e-19163.50Cytochrome P450 734A6 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A6 PE=2 ... [more]
Q6Z6D67.8e-17756.93Cytochrome P450 734A2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A2 PE=2 ... [more]
Q69XM63.9e-16854.46Cytochrome P450 734A4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A4 PE=2 ... [more]
Q8LIF25.6e-15150.48Cytochrome P450 734A5 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A5 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1L5285.1e-27287.92cytochrome P450 734A1-like OS=Cucurbita maxima OX=3661 GN=LOC111499208 PE=3 SV=1[more]
A0A6J1EZI17.4e-27188.36cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111440975 PE=3 SV... [more]
A0A1S3CMW02.0e-26083.99cytochrome P450 734A1-like OS=Cucumis melo OX=3656 GN=LOC103502730 PE=3 SV=1[more]
A0A0A0LU202.5e-25884.24Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G305710 PE=3 SV=1[more]
A0A6J1H9G11.5e-25584.91cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111461725 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT2G26710.17.2e-21871.15Cytochrome P450 superfamily protein [more]
AT3G14620.13.3e-11440.80cytochrome P450, family 72, subfamily A, polypeptide 8 [more]
AT3G14610.11.1e-11240.31cytochrome P450, family 72, subfamily A, polypeptide 7 [more]
AT3G14680.11.5e-11141.77cytochrome P450, family 72, subfamily A, polypeptide 14 [more]
AT1G75130.13.8e-11041.12cytochrome P450, family 721, subfamily A, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 215..233
score: 21.49
coord: 350..376
score: 28.27
coord: 97..116
score: 29.15
coord: 470..480
score: 54.13
coord: 480..503
score: 35.59
coord: 330..347
score: 30.53
IPR001128Cytochrome P450PRINTSPR00385P450coord: 471..480
score: 56.5
coord: 341..358
score: 38.91
coord: 480..491
score: 39.24
coord: 394..405
score: 39.28
IPR001128Cytochrome P450PFAMPF00067p450coord: 90..513
e-value: 2.4E-85
score: 287.0
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 36..534
e-value: 2.6E-115
score: 387.7
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 83..530
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 18..527
NoneNo IPR availablePANTHERPTHR24282:SF63CYTOCHROMEA2coord: 18..527
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 473..482

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0034367.1Lag0034367.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010268 brassinosteroid homeostasis
biological_process GO:0016131 brassinosteroid metabolic process
biological_process GO:0009741 response to brassinosteroid
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function GO:0008395 steroid hydroxylase activity
molecular_function GO:0004497 monooxygenase activity