Lag0034355 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0034355
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Amino-acid racemase isoform 2
Location	chr3: 6621428 .. 6622686 (+)
RNA-Seq Expression	Lag0034355
Synteny	Lag0034355

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTGATGGAGGGATGGCCATGCCATTTTATGGACTGAATTGCCCAGCACCTATTCGACGAAATGCCAACAAGAGGATTACTCGCTTTAGAAGGAGATTAAACCAGTATTCATCTGTGCAGATTTCCTCTGTTATTCAAACTGATGAGAATGACAACTTACCAGGATCCAAGAAGGTATCAAGTTCAGGAAAATCTTTGTCAAAAAGTCGAACTCCTAAACCGCTTCTTGTCCAGCCAAATACTGTGGGTGTCATAGGAGGAGTGTCGGTTATTTCTACTCTACTTTTCTTGGAAAAGCTCGTGTGGTGGAGTTTGAAGGATGGACAAGAGAGCATACCTTTTGTTGTCTGCAGCGATCCAGCATTGGGCAAAGGGATTTCCCCTCTTGCTTCGTTGAGTACATTCAGCACAAGTTCTTCTCAACATGGTCATAGTGATGCTCCAATCATTGAGAATTTGAAGCGCAAAAGGGCGTTTCTCGAACACTCCGGAGCTCGGTGCCTAATTACACCTTGCCATCTTTCACATAAGTGGCTTGGTGACACAACTGAGAGCTGCAAATTACCTTTCCTTCATGTGGGAGATTGTGTTGCTATGGAGCTTAAAGAGGCTAAGCTTAAGCCGCTTGAAGCAGGGAGCAGTGTCCGGATTGGGCTGCTTGCGACTGATAAAGCAATAGTAACTGGTTTTTACCATGAGAGGCTACAAAATCAGGTTTTGTAAATACATTCTCAACTAATGGTCGATACTCTAATTTACTTGATAAAGAGTGATGCACAAGCAATAAGCAGCAACGCATTAACAAAATCTATGCTTTATACTAATCTCTGAGCATTCAGAAAAATGGATAACTTTGCCATGCCTCATAATTAAAATGGATGTTGTTGAAGTGATTGAGGGCATTTTTCTACTCTGTTCTGTAGATGATTCCAAACTTTTCTTCTGATTTCCTTTTCTTTCTTCAGGGCTTTGATGTTGTCTTGCCAGACGAAGCCACCATGAAACATATTGTAGTTCCTGCAGTTGAAGCTTTCAACAAAAGGGATCAGGAAGGAGCAAGAAATCTGTTGAGAATTGCTGTTCATGTGCTCTTGATAAGAGCTGTGAATATGGTAATACTTGCTTCTGATGAATTGCTTAATCTTCTTCCTCCTGATGATCCTATTCTGAAAAAATGTATTGACCCCATGGATGCGTTGGCCAGAGCAGCCATTAAATGGTCTAGATCTACAGGAAATTTACATGAGAAAGCTTAG

mRNA sequence

ATGCTTGATGGAGGGATGGCCATGCCATTTTATGGACTGAATTGCCCAGCACCTATTCGACGAAATGCCAACAAGAGGATTACTCGCTTTAGAAGGAGATTAAACCAGTATTCATCTGTGCAGATTTCCTCTGTTATTCAAACTGATGAGAATGACAACTTACCAGGATCCAAGAAGGTATCAAGTTCAGGAAAATCTTTGTCAAAAAGTCGAACTCCTAAACCGCTTCTTGTCCAGCCAAATACTGTGGGTGTCATAGGAGGAGTGTCGGTTATTTCTACTCTACTTTTCTTGGAAAAGCTCGTGTGGTGGAGTTTGAAGGATGGACAAGAGAGCATACCTTTTGTTGTCTGCAGCGATCCAGCATTGGGCAAAGGGATTTCCCCTCTTGCTTCGTTGAGTACATTCAGCACAAGTTCTTCTCAACATGGTCATAGTGATGCTCCAATCATTGAGAATTTGAAGCGCAAAAGGGCGTTTCTCGAACACTCCGGAGCTCGGTGCCTAATTACACCTTGCCATCTTTCACATAAGTGGCTTGGTGACACAACTGAGAGCTGCAAATTACCTTTCCTTCATGTGGGAGATTGTGTTGCTATGGAGCTTAAAGAGGCTAAGCTTAAGCCGCTTGAAGCAGGGAGCAGTGTCCGGATTGGGCTGCTTGCGACTGATAAAGCAATAGTAACTGGTTTTTACCATGAGAGGCTACAAAATCAGGGCTTTGATGTTGTCTTGCCAGACGAAGCCACCATGAAACATATTGTAGTTCCTGCAGTTGAAGCTTTCAACAAAAGGGATCAGGAAGGAGCAAGAAATCTGTTGAGAATTGCTGTTCATGTGCTCTTGATAAGAGCTGTGAATATGGTAATACTTGCTTCTGATGAATTGCTTAATCTTCTTCCTCCTGATGATCCTATTCTGAAAAAATGTATTGACCCCATGGATGCGTTGGCCAGAGCAGCCATTAAATGGTCTAGATCTACAGGAAATTTACATGAGAAAGCTTAG

Coding sequence (CDS)

ATGCTTGATGGAGGGATGGCCATGCCATTTTATGGACTGAATTGCCCAGCACCTATTCGACGAAATGCCAACAAGAGGATTACTCGCTTTAGAAGGAGATTAAACCAGTATTCATCTGTGCAGATTTCCTCTGTTATTCAAACTGATGAGAATGACAACTTACCAGGATCCAAGAAGGTATCAAGTTCAGGAAAATCTTTGTCAAAAAGTCGAACTCCTAAACCGCTTCTTGTCCAGCCAAATACTGTGGGTGTCATAGGAGGAGTGTCGGTTATTTCTACTCTACTTTTCTTGGAAAAGCTCGTGTGGTGGAGTTTGAAGGATGGACAAGAGAGCATACCTTTTGTTGTCTGCAGCGATCCAGCATTGGGCAAAGGGATTTCCCCTCTTGCTTCGTTGAGTACATTCAGCACAAGTTCTTCTCAACATGGTCATAGTGATGCTCCAATCATTGAGAATTTGAAGCGCAAAAGGGCGTTTCTCGAACACTCCGGAGCTCGGTGCCTAATTACACCTTGCCATCTTTCACATAAGTGGCTTGGTGACACAACTGAGAGCTGCAAATTACCTTTCCTTCATGTGGGAGATTGTGTTGCTATGGAGCTTAAAGAGGCTAAGCTTAAGCCGCTTGAAGCAGGGAGCAGTGTCCGGATTGGGCTGCTTGCGACTGATAAAGCAATAGTAACTGGTTTTTACCATGAGAGGCTACAAAATCAGGGCTTTGATGTTGTCTTGCCAGACGAAGCCACCATGAAACATATTGTAGTTCCTGCAGTTGAAGCTTTCAACAAAAGGGATCAGGAAGGAGCAAGAAATCTGTTGAGAATTGCTGTTCATGTGCTCTTGATAAGAGCTGTGAATATGGTAATACTTGCTTCTGATGAATTGCTTAATCTTCTTCCTCCTGATGATCCTATTCTGAAAAAATGTATTGACCCCATGGATGCGTTGGCCAGAGCAGCCATTAAATGGTCTAGATCTACAGGAAATTTACATGAGAAAGCTTAG

Protein sequence

MLDGGMAMPFYGLNCPAPIRRNANKRITRFRRRLNQYSSVQISSVIQTDENDNLPGSKKVSSSGKSLSKSRTPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQESIPFVVCSDPALGKGISPLASLSTFSTSSSQHGHSDAPIIENLKRKRAFLEHSGARCLITPCHLSHKWLGDTTESCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQGFDVVLPDEATMKHIVVPAVEAFNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLLPPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA

Homology

[Close]

BLAST of Lag0034355 vs. NCBI nr
Match: XP_038878952.1 (uncharacterized protein LOC120071036 [Benincasa hispida])

HSP 1 Score: 599.7 bits (1545), Expect = 1.5e-167
Identity = 298/335 (88.96%), Postives = 312/335 (93.13%), Query Frame = 0


Query: 1   MLDGGMAMPFYGLNCPAPIRRNANKRITRFRRRLNQYSSVQISSVIQTDENDNLPGSKKV 60
           MLDGGM   FY L CPAPIRRNAN+ ITRFRRR+N YSSVQISSVIQTD NDNLPGSKK+
Sbjct: 1   MLDGGMTTSFYALTCPAPIRRNANEGITRFRRRINPYSSVQISSVIQTDANDNLPGSKKI 60

Query: 61  SSSGKSLSKSRTPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQESIPFVVCSD 120
           SSSGKSLSK+RTPKPLLVQP TVGVIGGVSV STLLFLEKLVWWS+KDGQESIPFVVCSD
Sbjct: 61  SSSGKSLSKTRTPKPLLVQPKTVGVIGGVSVFSTLLFLEKLVWWSMKDGQESIPFVVCSD 120

Query: 121 PALGKGISPLASLSTFSTSSSQHGHSDAPIIENLKRKRAFLEHSGARCLITPCHLSHKWL 180
           PALGKGI PL S  TFST+SSQ+GHSD PIIENLKRKRAFLE SGARCLITPCHL+H+WL
Sbjct: 121 PALGKGIPPLTSSCTFSTNSSQYGHSDDPIIENLKRKRAFLEQSGARCLITPCHLTHRWL 180

Query: 181 GDTTESCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQG 240
           GDT+ESCKLPFLHVGDCVA ELKEAKLKPLE GSSVRIGLLATD A V GFYHERLQNQG
Sbjct: 181 GDTSESCKLPFLHVGDCVARELKEAKLKPLEGGSSVRIGLLATDTATVAGFYHERLQNQG 240

Query: 241 FDVVLPDEATMKHIVVPAVEAFNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL 300
           FD++LPDEATM+HIV+PAVEA NKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL
Sbjct: 241 FDILLPDEATMEHIVIPAVEALNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL 300

Query: 301 PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 336
           PPDDPILKKCIDPMDALARAAIKWSRST NLHEKA
Sbjct: 301 PPDDPILKKCIDPMDALARAAIKWSRSTENLHEKA 335

[Close]

BLAST of Lag0034355 vs. NCBI nr
Match: XP_023006388.1 (uncharacterized protein LOC111499133 isoform X1 [Cucurbita maxima])

HSP 1 Score: 590.5 bits (1521), Expect = 9.1e-165
Identity = 295/335 (88.06%), Postives = 314/335 (93.73%), Query Frame = 0


Query: 1   MLDGGMAMPFYGLNCPAPIRRNANKRITRFRRRLNQYSSVQISSVIQTDENDNLPGSKKV 60
           MLDGGMAMPFY LNCPAP+RRNAN+ ITRFRRRLN YSS+QI SV+QTDENDNLP SKK+
Sbjct: 1   MLDGGMAMPFYALNCPAPVRRNANESITRFRRRLNPYSSLQIFSVVQTDENDNLPVSKKI 60

Query: 61  SSSGKSLSKSRTPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQESIPFVVCSD 120
           SS+GKS+SK R PKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQ+SIPFVVCSD
Sbjct: 61  SSAGKSVSKGRAPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQQSIPFVVCSD 120

Query: 121 PALGKGISPLASLSTFSTSSSQHGHSDAPIIENLKRKRAFLEHSGARCLITPCHLSHKWL 180
           PALGKGI PL SL+TF+ +SSQH  +DAPIIE LK+K  FLE SGARCLITPCHLSH+WL
Sbjct: 121 PALGKGIPPLTSLTTFTANSSQHAQTDAPIIEILKQKCVFLEQSGARCLITPCHLSHRWL 180

Query: 181 GDTTESCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQG 240
           GDT  SCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATD AIVTGFY ERLQNQG
Sbjct: 181 GDT--SCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDAAIVTGFYRERLQNQG 240

Query: 241 FDVVLPDEATMKHIVVPAVEAFNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL 300
           F+VVLPDEAT+KHI+VPAVEA NKRDQEGARNLLRIAVH+LLIRAVN+VILASDELLNLL
Sbjct: 241 FEVVLPDEATIKHILVPAVEALNKRDQEGARNLLRIAVHILLIRAVNIVILASDELLNLL 300

Query: 301 PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 336
           PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA
Sbjct: 301 PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 333

[Close]

BLAST of Lag0034355 vs. NCBI nr
Match: XP_023530993.1 (uncharacterized protein LOC111793383 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 587.8 bits (1514), Expect = 5.9e-164
Identity = 294/335 (87.76%), Postives = 312/335 (93.13%), Query Frame = 0


Query: 1   MLDGGMAMPFYGLNCPAPIRRNANKRITRFRRRLNQYSSVQISSVIQTDENDNLPGSKKV 60
           MLDGGMAMPFY LNCPAP+RRNAN+ ITRFRRRLN YSS+QI SV+QTDENDNLP SKK+
Sbjct: 2   MLDGGMAMPFYALNCPAPVRRNANESITRFRRRLNPYSSLQIFSVVQTDENDNLPVSKKI 61

Query: 61  SSSGKSLSKSRTPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQESIPFVVCSD 120
           S+ GKS+SK R PKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQ+SIPFVVCSD
Sbjct: 62  STPGKSVSKGRAPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQQSIPFVVCSD 121

Query: 121 PALGKGISPLASLSTFSTSSSQHGHSDAPIIENLKRKRAFLEHSGARCLITPCHLSHKWL 180
           PALGKGI PL SL+TF  +SSQH  +DAPIIE LK+K  FLE SGARCLITPCHLSH+WL
Sbjct: 122 PALGKGIPPLTSLTTFPANSSQHAQTDAPIIEILKQKCVFLEQSGARCLITPCHLSHRWL 181

Query: 181 GDTTESCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQG 240
           GDT  SCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATD AIVTGFY ERLQNQG
Sbjct: 182 GDT--SCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDAAIVTGFYRERLQNQG 241

Query: 241 FDVVLPDEATMKHIVVPAVEAFNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL 300
           F+VVLPDEAT+KHI+VPAVEA NKRDQEGARNLLRIAVH+LLIRAVN+VILASDELLNLL
Sbjct: 242 FEVVLPDEATIKHILVPAVEALNKRDQEGARNLLRIAVHILLIRAVNIVILASDELLNLL 301

Query: 301 PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 336
           PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA
Sbjct: 302 PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 334

[Close]

BLAST of Lag0034355 vs. NCBI nr
Match: XP_022931647.1 (uncharacterized protein LOC111437803 isoform X1 [Cucurbita moschata])

HSP 1 Score: 585.5 bits (1508), Expect = 2.9e-163
Identity = 293/335 (87.46%), Postives = 311/335 (92.84%), Query Frame = 0


Query: 1   MLDGGMAMPFYGLNCPAPIRRNANKRITRFRRRLNQYSSVQISSVIQTDENDNLPGSKKV 60
           MLDGGMAMPFY LNCPAP+RRNAN+ ITRFRRRLN YSS+QI SV+QTDENDNLP SKK+
Sbjct: 1   MLDGGMAMPFYALNCPAPVRRNANESITRFRRRLNPYSSLQIFSVVQTDENDNLPVSKKI 60

Query: 61  SSSGKSLSKSRTPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQESIPFVVCSD 120
           S+ GKS+SK R PKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQ+SIPFVVCSD
Sbjct: 61  STPGKSVSKGRAPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQQSIPFVVCSD 120

Query: 121 PALGKGISPLASLSTFSTSSSQHGHSDAPIIENLKRKRAFLEHSGARCLITPCHLSHKWL 180
           PALGKGI PL SL+TF  +SSQH  +DAPIIE LK+K  FLE SGARCLITPCHLSH+WL
Sbjct: 121 PALGKGIPPLTSLTTFPANSSQHAQTDAPIIEILKQKCVFLEQSGARCLITPCHLSHRWL 180

Query: 181 GDTTESCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQG 240
            DT  SCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATD AIVTGFY ERLQNQG
Sbjct: 181 SDT--SCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDAAIVTGFYRERLQNQG 240

Query: 241 FDVVLPDEATMKHIVVPAVEAFNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL 300
           F+VVLPDEAT+KHI+VPAVEA NKRDQEGARNLLRIAVH+LLIRAVN+VILASDELLNLL
Sbjct: 241 FEVVLPDEATIKHILVPAVEALNKRDQEGARNLLRIAVHILLIRAVNIVILASDELLNLL 300

Query: 301 PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 336
           PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA
Sbjct: 301 PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 333

[Close]

BLAST of Lag0034355 vs. NCBI nr
Match: KAG7021803.1 (hypothetical protein SDJN02_15531, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 582.8 bits (1501), Expect = 1.9e-162
Identity = 291/335 (86.87%), Postives = 311/335 (92.84%), Query Frame = 0


Query: 1   MLDGGMAMPFYGLNCPAPIRRNANKRITRFRRRLNQYSSVQISSVIQTDENDNLPGSKKV 60
           MLDGGMA+PFY LNCPAP+RRNAN+ ITRFRRRLN YSS+QI SV+QTDENDNLP SKK+
Sbjct: 1   MLDGGMAVPFYALNCPAPVRRNANESITRFRRRLNPYSSLQIFSVVQTDENDNLPVSKKI 60

Query: 61  SSSGKSLSKSRTPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQESIPFVVCSD 120
           S+ GKS+SK R PKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQ+SIPFVVCSD
Sbjct: 61  STPGKSVSKGRAPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQQSIPFVVCSD 120

Query: 121 PALGKGISPLASLSTFSTSSSQHGHSDAPIIENLKRKRAFLEHSGARCLITPCHLSHKWL 180
           PALGKGI PL SL+TF+ +SSQH   DAPIIE LK+K  FLE SGARCLITPCHLSH+WL
Sbjct: 121 PALGKGIPPLTSLTTFTENSSQHAQIDAPIIEILKQKCVFLEQSGARCLITPCHLSHRWL 180

Query: 181 GDTTESCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQG 240
           GDT  SCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATD AIVTGFY ERLQNQG
Sbjct: 181 GDT--SCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDAAIVTGFYRERLQNQG 240

Query: 241 FDVVLPDEATMKHIVVPAVEAFNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL 300
           F+VVLPD+AT+KHI+VPAVEA NKRDQEGARNLLRIAVH+LLIRAVN+VILASDE LNLL
Sbjct: 241 FEVVLPDDATIKHILVPAVEALNKRDQEGARNLLRIAVHILLIRAVNIVILASDEFLNLL 300

Query: 301 PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 336
           PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA
Sbjct: 301 PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 333

[Close]

BLAST of Lag0034355 vs. ExPASy Swiss-Prot
Match: O58403 (Aspartate racemase OS=Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) OX=70601 GN=PH0670 PE=1 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 2.4e-14
Identity = 67/251 (26.69%), Postives = 109/251 (43.43%), Query Frame = 0


Query: 82  TVGVIGGVSVISTL-LFLEKLVWWSLKDGQESIPFVVCSDP--------ALGKGISPLAS 141
           T+G++GG+  ++T  LF   ++    K  QE    ++ ++P         LGKG  P   
Sbjct: 3   TIGILGGMGPLATAELFRRIVIKTPAKRDQEHPKVIIFNNPQIPDRTAYILGKGEDPRPQ 62

Query: 142 LSTFSTSSSQHGHSDAPIIENLKRKRAFLEHSGARCLITPCHLSHKWLGDTTESCKLPFL 201
           L                 I   KR    LE  GA  +I PC+ +H ++ D  ++ K+P +
Sbjct: 63  L-----------------IWTAKR----LEECGADFIIMPCNTAHAFVEDIRKAIKIPII 122

Query: 202 HVGDCVAMELKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQGFDVVLPDEATMK 261
            + +  A ++KE   K        + GLLAT   IV+G Y +     G +++ P E   K
Sbjct: 123 SMIEETAKKVKELGFK--------KAGLLATTGTIVSGVYEKEFSKYGVEIMTPTEDEQK 182

Query: 262 HIVVPAVEAFNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLLPPDDPILKKCID 321
            ++    E     + +  R LL     +L  R    +I    E+  +L  DD +    ID
Sbjct: 183 DVMRGIYEGVKAGNLKLGRELLLKTAKILEERGAECIIAGCTEVSVVLKQDD-LKVPLID 223

Query: 322 PMDALARAAIK 324
           PMD +A  A+K
Sbjct: 243 PMDVIAEVAVK 223

[Close]

BLAST of Lag0034355 vs. ExPASy TrEMBL
Match: A0A6J1L4S4 (uncharacterized protein LOC111499133 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499133 PE=4 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 4.4e-165
Identity = 295/335 (88.06%), Postives = 314/335 (93.73%), Query Frame = 0


Query: 1   MLDGGMAMPFYGLNCPAPIRRNANKRITRFRRRLNQYSSVQISSVIQTDENDNLPGSKKV 60
           MLDGGMAMPFY LNCPAP+RRNAN+ ITRFRRRLN YSS+QI SV+QTDENDNLP SKK+
Sbjct: 1   MLDGGMAMPFYALNCPAPVRRNANESITRFRRRLNPYSSLQIFSVVQTDENDNLPVSKKI 60

Query: 61  SSSGKSLSKSRTPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQESIPFVVCSD 120
           SS+GKS+SK R PKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQ+SIPFVVCSD
Sbjct: 61  SSAGKSVSKGRAPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQQSIPFVVCSD 120

Query: 121 PALGKGISPLASLSTFSTSSSQHGHSDAPIIENLKRKRAFLEHSGARCLITPCHLSHKWL 180
           PALGKGI PL SL+TF+ +SSQH  +DAPIIE LK+K  FLE SGARCLITPCHLSH+WL
Sbjct: 121 PALGKGIPPLTSLTTFTANSSQHAQTDAPIIEILKQKCVFLEQSGARCLITPCHLSHRWL 180

Query: 181 GDTTESCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQG 240
           GDT  SCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATD AIVTGFY ERLQNQG
Sbjct: 181 GDT--SCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDAAIVTGFYRERLQNQG 240

Query: 241 FDVVLPDEATMKHIVVPAVEAFNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL 300
           F+VVLPDEAT+KHI+VPAVEA NKRDQEGARNLLRIAVH+LLIRAVN+VILASDELLNLL
Sbjct: 241 FEVVLPDEATIKHILVPAVEALNKRDQEGARNLLRIAVHILLIRAVNIVILASDELLNLL 300

Query: 301 PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 336
           PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA
Sbjct: 301 PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 333

[Close]

BLAST of Lag0034355 vs. ExPASy TrEMBL
Match: A0A6J1EU92 (uncharacterized protein LOC111437803 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111437803 PE=4 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 1.4e-163
Identity = 293/335 (87.46%), Postives = 311/335 (92.84%), Query Frame = 0


Query: 1   MLDGGMAMPFYGLNCPAPIRRNANKRITRFRRRLNQYSSVQISSVIQTDENDNLPGSKKV 60
           MLDGGMAMPFY LNCPAP+RRNAN+ ITRFRRRLN YSS+QI SV+QTDENDNLP SKK+
Sbjct: 1   MLDGGMAMPFYALNCPAPVRRNANESITRFRRRLNPYSSLQIFSVVQTDENDNLPVSKKI 60

Query: 61  SSSGKSLSKSRTPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQESIPFVVCSD 120
           S+ GKS+SK R PKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQ+SIPFVVCSD
Sbjct: 61  STPGKSVSKGRAPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQQSIPFVVCSD 120

Query: 121 PALGKGISPLASLSTFSTSSSQHGHSDAPIIENLKRKRAFLEHSGARCLITPCHLSHKWL 180
           PALGKGI PL SL+TF  +SSQH  +DAPIIE LK+K  FLE SGARCLITPCHLSH+WL
Sbjct: 121 PALGKGIPPLTSLTTFPANSSQHAQTDAPIIEILKQKCVFLEQSGARCLITPCHLSHRWL 180

Query: 181 GDTTESCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQG 240
            DT  SCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATD AIVTGFY ERLQNQG
Sbjct: 181 SDT--SCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDAAIVTGFYRERLQNQG 240

Query: 241 FDVVLPDEATMKHIVVPAVEAFNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL 300
           F+VVLPDEAT+KHI+VPAVEA NKRDQEGARNLLRIAVH+LLIRAVN+VILASDELLNLL
Sbjct: 241 FEVVLPDEATIKHILVPAVEALNKRDQEGARNLLRIAVHILLIRAVNIVILASDELLNLL 300

Query: 301 PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 336
           PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA
Sbjct: 301 PPDDPILKKCIDPMDALARAAIKWSRSTGNLHEKA 333

[Close]

BLAST of Lag0034355 vs. ExPASy TrEMBL
Match: A0A5A7TXR8 (Amino-acid racemase isoform 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold216G00390 PE=4 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 1.4e-158
Identity = 287/331 (86.71%), Postives = 303/331 (91.54%), Query Frame = 0


Query: 1   MLDGGMAMPFYGLNCPAPIRRNANKRITRFRRRLNQYSSVQISSVIQTDENDNLPGSKKV 60
           ML GGMAM FY   CPAP+RRNAN+ ITRFRRR+N YSSVQISSV+QTD NDNLP SKK+
Sbjct: 2   MLHGGMAMHFYAPICPAPVRRNANEGITRFRRRINLYSSVQISSVVQTDANDNLPRSKKI 61

Query: 61  SSSGKSLSKSRTPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQESIPFVVCSD 120
           SS GKSLSK+RTPKPLLVQPNTVGVIGGVSV STLLFLEKLVWWSLKDGQESIPFVVCS+
Sbjct: 62  SSLGKSLSKTRTPKPLLVQPNTVGVIGGVSVFSTLLFLEKLVWWSLKDGQESIPFVVCSE 121

Query: 121 PALGKGISPLASLSTFSTSSSQHGHSDAPIIENLKRKRAFLEHSGARCLITPCHLSHKWL 180
           PALGKGI P+ SL TFST SSQ+GHSDAPIIENL RKRAFLE SGARCLITPCHL+H+WL
Sbjct: 122 PALGKGIPPVTSLCTFSTDSSQYGHSDAPIIENLNRKRAFLELSGARCLITPCHLTHRWL 181

Query: 181 GDTTESCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQG 240
            DT+ESCKLPFLHVGDCVA ELKEA LKPLE G +VRIGLLATD   VTG Y+ERLQNQG
Sbjct: 182 SDTSESCKLPFLHVGDCVARELKEATLKPLEGGRNVRIGLLATD-TTVTGIYYERLQNQG 241

Query: 241 FDVVLPDEATMKHIVVPAVEAFNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL 300
           FDV+LPDEATMKHIV+PAVEA NKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL
Sbjct: 242 FDVLLPDEATMKHIVIPAVEALNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL 301

Query: 301 PPDDPILKKCIDPMDALARAAIKWSRSTGNL 332
           PPDDPILKKCIDPMDALARAAIKWSRST NL
Sbjct: 302 PPDDPILKKCIDPMDALARAAIKWSRSTENL 331

[Close]

BLAST of Lag0034355 vs. ExPASy TrEMBL
Match: A0A1S3CMU2 (uncharacterized protein LOC103502741 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502741 PE=4 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 1.4e-158
Identity = 287/331 (86.71%), Postives = 303/331 (91.54%), Query Frame = 0


Query: 1   MLDGGMAMPFYGLNCPAPIRRNANKRITRFRRRLNQYSSVQISSVIQTDENDNLPGSKKV 60
           ML GGMAM FY   CPAP+RRNAN+ ITRFRRR+N YSSVQISSV+QTD NDNLP SKK+
Sbjct: 2   MLHGGMAMHFYAPICPAPVRRNANEGITRFRRRINLYSSVQISSVVQTDANDNLPRSKKI 61

Query: 61  SSSGKSLSKSRTPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQESIPFVVCSD 120
           SS GKSLSK+RTPKPLLVQPNTVGVIGGVSV STLLFLEKLVWWSLKDGQESIPFVVCS+
Sbjct: 62  SSLGKSLSKTRTPKPLLVQPNTVGVIGGVSVFSTLLFLEKLVWWSLKDGQESIPFVVCSE 121

Query: 121 PALGKGISPLASLSTFSTSSSQHGHSDAPIIENLKRKRAFLEHSGARCLITPCHLSHKWL 180
           PALGKGI P+ SL TFST SSQ+GHSDAPIIENL RKRAFLE SGARCLITPCHL+H+WL
Sbjct: 122 PALGKGIPPVTSLCTFSTDSSQYGHSDAPIIENLNRKRAFLELSGARCLITPCHLTHRWL 181

Query: 181 GDTTESCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQG 240
            DT+ESCKLPFLHVGDCVA ELKEA LKPLE G +VRIGLLATD   VTG Y+ERLQNQG
Sbjct: 182 SDTSESCKLPFLHVGDCVARELKEATLKPLEGGRNVRIGLLATD-TTVTGIYYERLQNQG 241

Query: 241 FDVVLPDEATMKHIVVPAVEAFNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL 300
           FDV+LPDEATMKHIV+PAVEA NKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL
Sbjct: 242 FDVLLPDEATMKHIVIPAVEALNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL 301

Query: 301 PPDDPILKKCIDPMDALARAAIKWSRSTGNL 332
           PPDDPILKKCIDPMDALARAAIKWSRST NL
Sbjct: 302 PPDDPILKKCIDPMDALARAAIKWSRSTENL 331

[Close]

BLAST of Lag0034355 vs. ExPASy TrEMBL
Match: A0A5D3E266 (Amino-acid racemase isoform 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold64G00430 PE=4 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 2.6e-157
Identity = 286/331 (86.40%), Postives = 302/331 (91.24%), Query Frame = 0


Query: 1   MLDGGMAMPFYGLNCPAPIRRNANKRITRFRRRLNQYSSVQISSVIQTDENDNLPGSKKV 60
           ML GGMAM FY    PAP+RRNAN+ ITRFRRR+N YSSVQISSV+QTD NDNLP SKK+
Sbjct: 2   MLHGGMAMHFYAPIFPAPVRRNANEGITRFRRRINLYSSVQISSVVQTDANDNLPRSKKI 61

Query: 61  SSSGKSLSKSRTPKPLLVQPNTVGVIGGVSVISTLLFLEKLVWWSLKDGQESIPFVVCSD 120
           SS GKSLSK+RTPKPLLVQPNTVGVIGGVSV STLLFLEKLVWWSLKDGQESIPFVVCS+
Sbjct: 62  SSLGKSLSKTRTPKPLLVQPNTVGVIGGVSVFSTLLFLEKLVWWSLKDGQESIPFVVCSE 121

Query: 121 PALGKGISPLASLSTFSTSSSQHGHSDAPIIENLKRKRAFLEHSGARCLITPCHLSHKWL 180
           PALGKGI P+ SL TFST SSQ+GHSDAPIIENL RKRAFLE SGARCLITPCHL+H+WL
Sbjct: 122 PALGKGIPPVTSLCTFSTDSSQYGHSDAPIIENLNRKRAFLELSGARCLITPCHLTHRWL 181

Query: 181 GDTTESCKLPFLHVGDCVAMELKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQG 240
            DT+ESCKLPFLHVGDCVA ELKEA LKPLE G +VRIGLLATD   VTG Y+ERLQNQG
Sbjct: 182 SDTSESCKLPFLHVGDCVARELKEATLKPLEGGRNVRIGLLATD-TTVTGIYYERLQNQG 241

Query: 241 FDVVLPDEATMKHIVVPAVEAFNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL 300
           FDV+LPDEATMKHIV+PAVEA NKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL
Sbjct: 242 FDVLLPDEATMKHIVIPAVEALNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLL 301

Query: 301 PPDDPILKKCIDPMDALARAAIKWSRSTGNL 332
           PPDDPILKKCIDPMDALARAAIKWSRST NL
Sbjct: 302 PPDDPILKKCIDPMDALARAAIKWSRSTENL 331

[Close]

BLAST of Lag0034355 vs. TAIR 10
Match: AT1G15410.1 (aspartate-glutamate racemase family )

HSP 1 Score: 298.9 bits (764), Expect = 5.1e-81
Identity = 154/306 (50.33%), Postives = 214/306 (69.93%), Query Frame = 0


Query: 31  RRRLNQYSSVQISSV-IQTDENDNLPGSKK---VSSSGKSLSKSRTPKPLLVQPNTVGVI 90
           R RL++  ++  SSV +  DE+++LP  KK   +S   ++   S     LL Q NTVG+I
Sbjct: 25  RVRLSRVLAMPPSSVLLHMDESNDLPKPKKGFCLSEDSRNSLNSLPADSLLRQANTVGII 84

Query: 91  GGVSVISTLLFLEKLVWWSLKDGQESIPFVVCSDPALGKGISPLASLSTFSTSSSQHGHS 150
           GGVS  STL F++KLV WS  DG+ S+PFV+CSDPAL K    L      S  S  H   
Sbjct: 85  GGVSTDSTLKFVKKLVDWSSNDGKSSLPFVLCSDPALNK---ELLLYEENSYPSLYHREE 144

Query: 151 DAP-----IIENLKRKRAFLEHSGARCLITPCHLSHKWLGDTTESCKLPFLHVGDCVAME 210
             P     I+ENL+ KR +LE  GA+ ++ PCH++H W  +  E   +P LH+G+C+A E
Sbjct: 145 STPVDPKLIVENLRNKRRYLERCGAKLILMPCHIAHIWYEEVCEGSLVPLLHMGECIAKE 204

Query: 211 LKEAKLKPLEAGSSVRIGLLATDKAIVTGFYHERLQNQGFDVVLPDEATMKHIVVPAVEA 270
           L+EAK+KPLEAG+ +R+G++AT   +  GFY E+LQ+ GF+ VLPD+ATM+H V+P++EA
Sbjct: 205 LQEAKMKPLEAGNPLRVGVMATSATLSAGFYQEKLQSNGFEAVLPDKATMEHTVIPSIEA 264

Query: 271 FNKRDQEGARNLLRIAVHVLLIRAVNMVILASDELLNLLPPDDPILKKCIDPMDALARAA 328
             ++D EGARNLLRIA+ VLL++AVN+V+L SDE+ +LLP DDP+LKKC+DPMDALAR+A
Sbjct: 265 MKRKDMEGARNLLRIALQVLLVQAVNVVMLGSDEMRDLLPGDDPLLKKCVDPMDALARSA 324

The following BLAST results are available for this feature:

BLAST of Lag0034355 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038878952.1	1.5e-167	88.96	uncharacterized protein LOC120071036 [Benincasa hispida]	[more]
XP_023006388.1	9.1e-165	88.06	uncharacterized protein LOC111499133 isoform X1 [Cucurbita maxima]	[more]
XP_023530993.1	5.9e-164	87.76	uncharacterized protein LOC111793383 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022931647.1	2.9e-163	87.46	uncharacterized protein LOC111437803 isoform X1 [Cucurbita moschata]	[more]
KAG7021803.1	1.9e-162	86.87	hypothetical protein SDJN02_15531, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of Lag0034355 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O58403	2.4e-14	26.69	Aspartate racemase OS=Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JC...	[more]

BLAST of Lag0034355 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1L4S4	4.4e-165	88.06	uncharacterized protein LOC111499133 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1EU92	1.4e-163	87.46	uncharacterized protein LOC111437803 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A5A7TXR8	1.4e-158	86.71	Amino-acid racemase isoform 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc...	[more]
A0A1S3CMU2	1.4e-158	86.71	uncharacterized protein LOC103502741 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A5D3E266	2.6e-157	86.40	Amino-acid racemase isoform 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]

BLAST of Lag0034355 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G15410.1	5.1e-81	50.33	aspartate-glutamate racemase family	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.1860		coord: 82..319 e-value: 3.4E-41 score: 143.4
None	No IPR available	GENE3D	3.40.50.1860		coord: 196..313 e-value: 3.4E-41 score: 143.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 48..73
None	No IPR available	PANTHER	PTHR21198:SF9	ASPARTATE RACEMASE	coord: 1..331
None	No IPR available	PANTHER	PTHR21198	GLUTAMATE RACEMASE	coord: 1..331
IPR015942	Asp/Glu/hydantoin racemase	PFAM	PF01177	Asp_Glu_race	coord: 86..321 e-value: 3.1E-24 score: 86.0
IPR001920	Asp/Glu racemase	SUPERFAMILY	53681	Aspartate/glutamate racemase	coord: 82..204
IPR001920	Asp/Glu racemase	SUPERFAMILY	53681	Aspartate/glutamate racemase	coord: 217..324

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0034355.1	Lag0034355.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006807	nitrogen compound metabolic process
molecular_function	GO:0036361	racemase activity, acting on amino acids and derivatives
molecular_function	GO:0016855	racemase and epimerase activity, acting on amino acids and derivatives