Lag0034329 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0034329
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionEpidermal patterning factor-like protein
Locationchr3: 6358983 .. 6359396 (-)
RNA-Seq ExpressionLag0034329
SyntenyLag0034329
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTAAAAAACTCTCTCCTTCTCATTCTCCCTCTCATATTCTCCCTCTTTCTCTCTCAACAACCCACCACTTCAGGTAATTAATCAGCCCACATAATCATTAATCCAATTCAGAGACTTTTATGATATCATCAGTTTAATTTTGTTGGGTTTGGTTTGGTTATTTTTCTGATTTCAGGGCATGAGGATTCAAAGCAGAAGGAATTGGTAATTGGGTCAAGGCCACCACAGTGCTTCAACAAGTGCTTAAATTGCAAGCCATGTGTTGCTGCTTTGGTCATCTCTCAGCGCCATGATACTAACACTGTTGTTCAAATCTCTAATGTGGAAGCGCTTCCACTCCCTAAAGATGATGCCTATTATCTTCTCTCATGGAAATGCAAGTGTAAAGATAAGTACTTTCAGCCTTGA

mRNA sequence

ATGGCTTTAAAAAACTCTCTCCTTCTCATTCTCCCTCTCATATTCTCCCTCTTTCTCTCTCAACAACCCACCACTTCAGGGCATGAGGATTCAAAGCAGAAGGAATTGGTAATTGGGTCAAGGCCACCACAGTGCTTCAACAAGTGCTTAAATTGCAAGCCATGTGTTGCTGCTTTGGTCATCTCTCAGCGCCATGATACTAACACTGTTGTTCAAATCTCTAATGTGGAAGCGCTTCCACTCCCTAAAGATGATGCCTATTATCTTCTCTCATGGAAATGCAAGTGTAAAGATAAGTACTTTCAGCCTTGA

Coding sequence (CDS)

ATGGCTTTAAAAAACTCTCTCCTTCTCATTCTCCCTCTCATATTCTCCCTCTTTCTCTCTCAACAACCCACCACTTCAGGGCATGAGGATTCAAAGCAGAAGGAATTGGTAATTGGGTCAAGGCCACCACAGTGCTTCAACAAGTGCTTAAATTGCAAGCCATGTGTTGCTGCTTTGGTCATCTCTCAGCGCCATGATACTAACACTGTTGTTCAAATCTCTAATGTGGAAGCGCTTCCACTCCCTAAAGATGATGCCTATTATCTTCTCTCATGGAAATGCAAGTGTAAAGATAAGTACTTTCAGCCTTGA

Protein sequence

MALKNSLLLILPLIFSLFLSQQPTTSGHEDSKQKELVIGSRPPQCFNKCLNCKPCVAALVISQRHDTNTVVQISNVEALPLPKDDAYYLLSWKCKCKDKYFQP
Homology
[Close]
BLAST of Lag0034329 vs. NCBI nr
Match: XP_022933441.1 (EPIDERMAL PATTERNING FACTOR-like protein 8 isoform X2 [Cucurbita moschata] >KAG7021781.1 EPIDERMAL PATTERNING FACTOR-like protein 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 161.0 bits (406), Expect = 5.5e-36
Identity = 82/106 (77.36%), Postives = 88/106 (83.02%), Query Frame = 0 

Query: 1   MALKNSLLLILPLIFSLFLSQQPTTSGHEDSKQKELVIGSRPPQCFNKCLNCKPCVAALV 60
           MAL NSL LILPLIFS  LS    TSG+EDSKQKEL IGSRPP+CFNKCLNCKPCVAALV
Sbjct: 1   MALNNSLFLILPLIFS--LSVFHPTSGNEDSKQKELAIGSRPPKCFNKCLNCKPCVAALV 60

Query: 61  ISQRH---DTNTVVQISNVEALPLPKDDAYYLLSWKCKCKDKYFQP 104
           ISQRH   D+N    +SN+EA  +PKDDAYYLLSWKCKCKDKYFQP
Sbjct: 61  ISQRHKGDDSNNGGVVSNLEAAQVPKDDAYYLLSWKCKCKDKYFQP 104

[Close]
BLAST of Lag0034329 vs. NCBI nr
Match: XP_023531007.1 (EPIDERMAL PATTERNING FACTOR-like protein 1 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 161.0 bits (406), Expect = 5.5e-36
Identity = 82/106 (77.36%), Postives = 88/106 (83.02%), Query Frame = 0 

Query: 1   MALKNSLLLILPLIFSLFLSQQPTTSGHEDSKQKELVIGSRPPQCFNKCLNCKPCVAALV 60
           MAL NSL LILPLIFS  LS    TSG+EDSKQKEL IGSRPP+CFNKCLNCKPCVAALV
Sbjct: 50  MALNNSLFLILPLIFS--LSVFHPTSGNEDSKQKELAIGSRPPKCFNKCLNCKPCVAALV 109

Query: 61  ISQRH---DTNTVVQISNVEALPLPKDDAYYLLSWKCKCKDKYFQP 104
           ISQRH   D+N    +SN+EA  +PKDDAYYLLSWKCKCKDKYFQP
Sbjct: 110 ISQRHKGDDSNNGGVVSNLEAAQVPKDDAYYLLSWKCKCKDKYFQP 153

[Close]
BLAST of Lag0034329 vs. NCBI nr
Match: XP_038879118.1 (EPIDERMAL PATTERNING FACTOR-like protein 8 [Benincasa hispida])

HSP 1 Score: 160.2 bits (404), Expect = 9.3e-36
Identity = 87/125 (69.60%), Postives = 92/125 (73.60%), Query Frame = 0 

Query: 1   MALKNSLLLILPLIFSLFLSQQPTTSGHED--------------------SKQKELVIGS 60
           MALKNSLLLILPLIF LF S    TSGHED                    SKQKELVIGS
Sbjct: 1   MALKNSLLLILPLIFCLFFSH--PTSGHEDSSPSSSSSSSSSSSSSSSSSSKQKELVIGS 60

Query: 61  RPPQCFNKCLNCKPCVAALVISQRHDTNTVVQISNVEA--LPLPKDDAYYLLSWKCKCKD 104
           RPPQCFNKCLNCKPCVAALVISQ H  + +V +SN+EA  LP+PKDDAYYLLSWKCKCKD
Sbjct: 61  RPPQCFNKCLNCKPCVAALVISQNHKNDDIV-VSNLEADLLPVPKDDAYYLLSWKCKCKD 120

[Close]
BLAST of Lag0034329 vs. NCBI nr
Match: XP_022933440.1 (EPIDERMAL PATTERNING FACTOR-like protein 8 isoform X1 [Cucurbita moschata] >KAG6587897.1 EPIDERMAL PATTERNING FACTOR-like protein 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 159.1 bits (401), Expect = 2.1e-35
Identity = 82/110 (74.55%), Postives = 90/110 (81.82%), Query Frame = 0 

Query: 1   MALKNSLLLILPLIFSLFLSQQPTT----SGHEDSKQKELVIGSRPPQCFNKCLNCKPCV 60
           MAL NSL LILPLIFSL +   PT+    SG+EDSKQKEL IGSRPP+CFNKCLNCKPCV
Sbjct: 1   MALNNSLFLILPLIFSLSVF-HPTSVLGISGNEDSKQKELAIGSRPPKCFNKCLNCKPCV 60

Query: 61  AALVISQRH---DTNTVVQISNVEALPLPKDDAYYLLSWKCKCKDKYFQP 104
           AALVISQRH   D+N    +SN+EA  +PKDDAYYLLSWKCKCKDKYFQP
Sbjct: 61  AALVISQRHKGDDSNNGGVVSNLEAAQVPKDDAYYLLSWKCKCKDKYFQP 109

[Close]
BLAST of Lag0034329 vs. NCBI nr
Match: XP_023531006.1 (EPIDERMAL PATTERNING FACTOR-like protein 1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 159.1 bits (401), Expect = 2.1e-35
Identity = 82/110 (74.55%), Postives = 90/110 (81.82%), Query Frame = 0 

Query: 1   MALKNSLLLILPLIFSLFLSQQPTT----SGHEDSKQKELVIGSRPPQCFNKCLNCKPCV 60
           MAL NSL LILPLIFSL +   PT+    SG+EDSKQKEL IGSRPP+CFNKCLNCKPCV
Sbjct: 50  MALNNSLFLILPLIFSLSVF-HPTSVLGISGNEDSKQKELAIGSRPPKCFNKCLNCKPCV 109

Query: 61  AALVISQRH---DTNTVVQISNVEALPLPKDDAYYLLSWKCKCKDKYFQP 104
           AALVISQRH   D+N    +SN+EA  +PKDDAYYLLSWKCKCKDKYFQP
Sbjct: 110 AALVISQRHKGDDSNNGGVVSNLEAAQVPKDDAYYLLSWKCKCKDKYFQP 158

[Close]
BLAST of Lag0034329 vs. ExPASy Swiss-Prot
Match: Q1G3V9 (EPIDERMAL PATTERNING FACTOR-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=EPFL8 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 5.0e-08
Identity = 34/98 (34.69%), Postives = 47/98 (47.96%), Query Frame = 0 

Query: 8   LLILPLIFSLFLSQQPTTSGH-EDSKQKELVIGSRPPQCFNKCLNCKPCVAALV-ISQRH 67
           L +  + FSL LS    +  H    + KE V+GS PP C  KC NCKPC+  L  I   H
Sbjct: 16  LFVANIFFSL-LSLHCISGAHGHQQRMKESVMGSEPPVCATKCRNCKPCLPYLFDIRGAH 75

Query: 68  DTNTVVQISNVEALPLPKDDAYYLLSWKCKCKDKYFQP 104
           D +                + YY + W C+C+D+ F+P
Sbjct: 76  DDD-------------DDSEPYYPVKWICRCRDRVFEP 99

[Close]
BLAST of Lag0034329 vs. ExPASy Swiss-Prot
Match: Q9LFT5 (EPIDERMAL PATTERNING FACTOR-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=EPFL1 PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 8.0e-06
Identity = 36/120 (30.00%), Postives = 50/120 (41.67%), Query Frame = 0 

Query: 2   ALKNSLLLILPLIFSLFLSQ---------QPTTSGHEDSKQKELVIGSRPPQCFNKCLNC 61
           A+  S LL+LPLI  L ++          QP  S      + +  +GS PP C N+C NC
Sbjct: 3   AIYKSTLLLLPLILILLITPQVSSFLQPIQPPISPQVALIEDKARLGSTPPSCHNRCNNC 62

Query: 62  KPCVAALV--ISQRHDTNTVVQISNVEALP-------LPKDDAYYLLSWKCKCKDKYFQP 104
            PC+A  V  +  R     V   S     P       L +   Y  + WKC C   ++ P
Sbjct: 63  HPCMAIQVPTLPTRSRFTRVNPFSGGFVRPPSSLTTVLDQYSNYKPMGWKCHCNGHFYNP 122

[Close]
BLAST of Lag0034329 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 1.5e-04
Identity = 24/76 (31.58%), Postives = 34/76 (44.74%), Query Frame = 0 

Query: 28  HEDSKQKELVIGSRPPQCFNKCLNCKPCVAALVISQRHDTNTVVQISNVEALPLPKDDAY 87
           ++   +  +++GSRPP C NKC  C PC A LVI  R                +P++  Y
Sbjct: 456 YDKGNRTRILLGSRPPNCTNKCHGCSPCKAKLVIVHR---------------IMPQE--Y 514

Query: 88  YLLSWKCKCKDKYFQP 104
           Y   W C    K + P
Sbjct: 516 YPQRWMCSRHGKIYHP 514

[Close]
BLAST of Lag0034329 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 3.4e-04
Identity = 27/91 (29.67%), Postives = 35/91 (38.46%), Query Frame = 0 

Query: 19  LSQQPTTSGHED------SKQKELVIGSRPPQCFNKCLNCKPCVAALVISQRHDTNTVVQ 78
           L QQPTT   E+           + + S PP C NKC  C+PC   L+I   +       
Sbjct: 441 LQQQPTTVMDENKGACAKGDSTCISLSSSPPNCKNKCKGCQPCKPKLIIVHPN------- 500

Query: 79  ISNVEALPLPKDDAYYLLSWKCKCKDKYFQP 104
                     K   YY   W C C +K + P
Sbjct: 501 ----------KPQDYYPQKWVCSCHNKIYNP 514

[Close]
BLAST of Lag0034329 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 5.7e-04
Identity = 28/87 (32.18%), Postives = 37/87 (42.53%), Query Frame = 0 

Query: 17  LFLSQQPTTSGHEDSKQKELVIGSRPPQCFNKCLNCKPCVAALVISQRHDTNTVVQISNV 76
           L L Q+PTT   ++     L   S PP C NKC  C+PC   L+I   +           
Sbjct: 440 LELHQKPTTLLMDEKMGASL--NSSPPNCKNKCKGCQPCKPKLIIVHPNQ---------- 499

Query: 77  EALPLPKDDAYYLLSWKCKCKDKYFQP 104
                P+D  YY   W C C +K + P
Sbjct: 500 -----PED--YYPQRWVCSCHNKIYNP 507

The following BLAST results are available for this feature:
BLAST of Lag0034329 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022933441.15.5e-3677.36EPIDERMAL PATTERNING FACTOR-like protein 8 isoform X2 [Cucurbita moschata] >KAG7... [more]
XP_023531007.15.5e-3677.36EPIDERMAL PATTERNING FACTOR-like protein 1 isoform X2 [Cucurbita pepo subsp. pep... [more]
XP_038879118.19.3e-3669.60EPIDERMAL PATTERNING FACTOR-like protein 8 [Benincasa hispida][more]
XP_022933440.12.1e-3574.55EPIDERMAL PATTERNING FACTOR-like protein 8 isoform X1 [Cucurbita moschata] >KAG6... [more]
XP_023531006.12.1e-3574.55EPIDERMAL PATTERNING FACTOR-like protein 1 isoform X1 [Cucurbita pepo subsp. pep... [more]
BLAST of Lag0034329 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q1G3V95.0e-0834.69EPIDERMAL PATTERNING FACTOR-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=EP... [more]
Q9LFT58.0e-0630.00EPIDERMAL PATTERNING FACTOR-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=EP... [more]
P432121.5e-0431.58Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
Q7M1E73.4e-0429.67Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
Q9FY195.7e-0432.18Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF17181EPFcoord: 37..103
e-value: 2.3E-18
score: 65.9
NoneNo IPR availablePANTHERPTHR33109:SF57EPIDERMAL PATTERNING FACTOR-LIKE PROTEINcoord: 5..103
IPR039455EPIDERMAL PATTERNING FACTOR-like proteinPANTHERPTHR33109EPIDERMAL PATTERNING FACTOR-LIKE PROTEIN 4coord: 5..103

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0034329.1Lag0034329.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010052 guard cell differentiation
biological_process GO:0010374 stomatal complex development
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane