Lag0034317 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0034317
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Phytocyanin domain-containing protein
Location	chr3: 6281296 .. 6282036 (-)
RNA-Seq Expression	Lag0034317
Synteny	Lag0034317

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGAATTGGGATATTTTTTGTGGTGGTGGTGGCAGCGACCACCGTGCTTCCGGCGGCGGTAGCGGTGGAGATTAACGTCGGAGGAGCAACTACTTGGCGGATTCCTTCTGACCCCAACTTTTATTCCTCATGGGTTAGCCGTGAAACGATTGCCCTTCACGACATCTTAGGTCTGATTTAAAAAATTATTCAACTTAGTCGTCAATAGAATTTTCTCCCCTCATAATATTTAATTTAATGTTATGTTAGTGATTTTCCTTGTTGATAATATTTAATATTATGTCAAAGAACTTTCTTTTAATACGTTTGTAAGTTTTTAGCAGTAACTTGACATGATATTAAAATAGCAGTCGTGTTTTAAGTACGTCAAATTTACGTTAACCTATCAACTTTACCTATAGTGTTCAGATTTGCAGCGGACAGCCATAACGTGGCTGGAGTGACAAAGGAGGCGTACGACAATTGCGACACCAGCAATCCGAAATTCGTCAATCACACAACGCCTTTCAGATTCAGGTTAGATAAGTCGGAGAATTACTACTTCATCTGCACCGTCGCCGCCACCTGCTCCGCCGGGCAGAAGTTGGCCATCACTAACATCGTCAAACAACGATCATCGACCGTCACTCCGTCACCACCGCCGCCTCCGTCGAGCTCTGCATCGACTCTGGCCTCCGTCACCTTCACTGTTGCCTTCATCTTCATTATTGCTGTAGCTCTAATTATTGCATTACAATAA

mRNA sequence

ATGAGAATTGGGATATTTTTTGTGGTGGTGGTGGCAGCGACCACCGTGCTTCCGGCGGCGGTAGCGGTGGAGATTAACGTCGGAGGAGCAACTACTTGGCGGATTCCTTCTGACCCCAACTTTTATTCCTCATGGGTTAGCCGTGAAACGATTGCCCTTCACGACATCTTAGTGTTCAGATTTGCAGCGGACAGCCATAACGTGGCTGGAGTGACAAAGGAGGCGTACGACAATTGCGACACCAGCAATCCGAAATTCGTCAATCACACAACGCCTTTCAGATTCAGGTTAGATAAGTCGGAGAATTACTACTTCATCTGCACCGTCGCCGCCACCTGCTCCGCCGGGCAGAAGTTGGCCATCACTAACATCGTCAAACAACGATCATCGACCGTCACTCCGTCACCACCGCCGCCTCCGTCGAGCTCTGCATCGACTCTGGCCTCCGTCACCTTCACTGTTGCCTTCATCTTCATTATTGCTGTAGCTCTAATTATTGCATTACAATAA

Coding sequence (CDS)

ATGAGAATTGGGATATTTTTTGTGGTGGTGGTGGCAGCGACCACCGTGCTTCCGGCGGCGGTAGCGGTGGAGATTAACGTCGGAGGAGCAACTACTTGGCGGATTCCTTCTGACCCCAACTTTTATTCCTCATGGGTTAGCCGTGAAACGATTGCCCTTCACGACATCTTAGTGTTCAGATTTGCAGCGGACAGCCATAACGTGGCTGGAGTGACAAAGGAGGCGTACGACAATTGCGACACCAGCAATCCGAAATTCGTCAATCACACAACGCCTTTCAGATTCAGGTTAGATAAGTCGGAGAATTACTACTTCATCTGCACCGTCGCCGCCACCTGCTCCGCCGGGCAGAAGTTGGCCATCACTAACATCGTCAAACAACGATCATCGACCGTCACTCCGTCACCACCGCCGCCTCCGTCGAGCTCTGCATCGACTCTGGCCTCCGTCACCTTCACTGTTGCCTTCATCTTCATTATTGCTGTAGCTCTAATTATTGCATTACAATAA

Protein sequence

MRIGIFFVVVVAATTVLPAAVAVEINVGGATTWRIPSDPNFYSSWVSRETIALHDILVFRFAADSHNVAGVTKEAYDNCDTSNPKFVNHTTPFRFRLDKSENYYFICTVAATCSAGQKLAITNIVKQRSSTVTPSPPPPPSSSASTLASVTFTVAFIFIIAVALIIALQ

Homology

[Close]

BLAST of Lag0034317 vs. NCBI nr
Match: XP_038878561.1 (umecyanin-like [Benincasa hispida])

HSP 1 Score: 154.1 bits (388), Expect = 1.1e-33
Identity = 92/181 (50.83%), Postives = 116/181 (64.09%), Query Frame = 0


Query: 1   MRIGIFFVVVVAATTVLPAAVAVEINVGGATTWRIPSDPNFYSSWVSRETIALHDILVFR 60
           MRIGI F+V V ATTV   A A+EI+VGG   W  PS P+FYS W +     + D+LVF 
Sbjct: 1   MRIGIVFLVTVVATTVRHVAEAIEIHVGGDGGWLRPSSPDFYSLWAAGLNFTVGDVLVFS 60

Query: 61  FAADSHNVAGVTKEAYDNCDTSNPKFVNHTTPFRFRLDKSENYYFICTVAATCSAGQKLA 120
           F   +HNVAGVTK+ YDNC+TSNPKF+N T+PF F L+  +++YFICTV   CSAGQKLA
Sbjct: 61  FQTGAHNVAGVTKDGYDNCNTSNPKFLNTTSPFSFTLETLDDFYFICTVPGHCSAGQKLA 120

Query: 121 ITNIVKQRSSTVTP---------------SPPPPPSSSASTLASVTFTVAFIFIIAVALI 167
           ITN+ +      +P               + PPPP+S   T+AS+ FTVA +  IAV LI
Sbjct: 121 ITNVQQSPPPGSSPPDSPPVSGEMPPPVVTAPPPPNSVTRTMASI-FTVALV-SIAVTLI 179

[Close]

BLAST of Lag0034317 vs. NCBI nr
Match: XP_008443977.1 (PREDICTED: umecyanin-like [Cucumis melo] >KAA0050151.1 umecyanin-like [Cucumis melo var. makuwa] >TYK06407.1 umecyanin-like [Cucumis melo var. makuwa])

HSP 1 Score: 149.8 bits (377), Expect = 2.1e-32
Identity = 90/187 (48.13%), Postives = 116/187 (62.03%), Query Frame = 0


Query: 3   IGIFFVVVVAATTVLPAAVAVEINVGGATTWRIPSDPNFYSSWVSRETIALHDILVFRFA 62
           +GI +VV + A TVL AA AV I VGG + W  P +PNFYSSW +     + DILVF FA
Sbjct: 4   VGIVYVVALVAITVLQAAEAVVIPVGGDSGWIRPPNPNFYSSWAAGLNFIVGDILVFNFA 63

Query: 63  ADSHNVAGVTKEAYDNCDTSNPKFVNHTTPFRFRLDKSENYYFICTVAATCSAGQKLAIT 122
              H+VAGVTK+ YDNC+T+NP F+N T+PF F LDK E+++FICT+   CSAGQKLAIT
Sbjct: 64  TGGHDVAGVTKDGYDNCNTNNPIFLNTTSPFSFTLDKLEDFFFICTIPGHCSAGQKLAIT 123

Query: 123 NIVKQRSSTVTPSPP-----------------------PPPSSSASTLASVTFTVAFIFI 167
           N+ +    T   SPP                       PPP +SA+++ +  FTVAF+  
Sbjct: 124 NLQQSPPPTSPNSPPVSGNEPPPTPPASPNSPPVSSEMPPPPNSATSIMASIFTVAFV-S 183

[Close]

BLAST of Lag0034317 vs. NCBI nr
Match: XP_008444048.1 (PREDICTED: umecyanin-like [Cucumis melo])

HSP 1 Score: 149.1 bits (375), Expect = 3.5e-32
Identity = 96/175 (54.86%), Postives = 120/175 (68.57%), Query Frame = 0


Query: 1   MRIGI-FFVVVVAATTVLPAAVAVEINVGGATTWRIPSDPNFYSSWVSRETIALHDILVF 60
           MRIGI F V  VAATTVL    A+EI +GG + W  P+DPN+YS+W   +   + D+LVF
Sbjct: 10  MRIGIVFLVAAVAATTVLHTGEALEIFIGGTSGWLRPNDPNWYSNWEDLK-FTVGDVLVF 69

Query: 61  RFAADSHNVAGVTKEAYDNCDTSNPKFVNHTTPFRFRLDKSENYYFICTVAATCSAGQKL 120
            F  D HNVAGVTKE YDNCDT+N KF+N T+PF F +   ++ +FICTV   CSAGQK+
Sbjct: 70  NFLMD-HNVAGVTKEGYDNCDTNNLKFINTTSPFLFTIKTLDDLFFICTVPGHCSAGQKI 129

Query: 121 AITNIVKQRSSTVTPSP--------PPPPSSSASTLASVTFTVAFIFIIAVALII 167
           AITNI  Q+SS+   SP        PPPP+S AS +AS+ FTVAF+  +AV LII
Sbjct: 130 AITNI--QQSSSTPSSPDSPTVVTAPPPPNSVASIMASI-FTVAFV-SMAVMLII 178

[Close]

BLAST of Lag0034317 vs. NCBI nr
Match: KAA0050152.1 (uncharacterized protein E6C27_scaffold675G001830 [Cucumis melo var. makuwa] >TYK06408.1 uncharacterized protein E5676_scaffold163G001090 [Cucumis melo var. makuwa])

HSP 1 Score: 148.3 bits (373), Expect = 6.0e-32
Identity = 91/173 (52.60%), Postives = 117/173 (67.63%), Query Frame = 0


Query: 1   MRIGI-FFVVVVAATTVLPAAVAVEINVGGATTWRIPSDPNFYSSWVSRETIALHDILVF 60
           MRIGI F V  VAATTVL    A+EI +GG + W  P+DPN+YS+W   +   + D+LVF
Sbjct: 1   MRIGIVFLVAAVAATTVLHTGEALEIFIGGTSGWLRPNDPNWYSNWEDLK-FTVGDVLVF 60

Query: 61  RFAADSHNVAGVTKEAYDNCDTSNPKFVNHTTPFRFRLDKSENYYFICTVAATCSAGQKL 120
            F  D HNVAGVTKE YDNCDT+N KF+N T+PF F +   ++ +FICTV   CSAGQK+
Sbjct: 61  NFLMD-HNVAGVTKEGYDNCDTNNLKFINTTSPFLFTIKTLDDLFFICTVPGHCSAGQKI 120

Query: 121 AITNIVKQRSSTVTPSP--------PPPPSSSASTLASVTFTVAFIFIIAVAL 165
           AITNI  Q+SS+   SP        PPPP+S AS +AS+ FTVAF+ +  + +
Sbjct: 121 AITNI--QQSSSTPSSPDSPTVVTAPPPPNSVASIMASI-FTVAFVSMAVMCI 168

[Close]

BLAST of Lag0034317 vs. NCBI nr
Match: XP_004150037.1 (cucumber peeling cupredoxin-like [Cucumis sativus] >KGN65338.1 hypothetical protein Csa_020086 [Cucumis sativus])

HSP 1 Score: 143.3 bits (360), Expect = 1.9e-30
Identity = 91/196 (46.43%), Postives = 117/196 (59.69%), Query Frame = 0


Query: 3   IGIFFVVVVAATTVLPAAVAVEINVGGATTWRIPSDPNFYSSWVSRETIALHDILVFRFA 62
           +GI FVV + ATTVL AA AV I VGG + W  P + +FYSSW +     + DILVF F 
Sbjct: 4   VGIVFVVALVATTVLQAAEAVVIPVGGDSGWIRPPNSDFYSSWAAGLKFTVGDILVFNFM 63

Query: 63  ADSHNVAGVTKEAYDNCDTSNPKFVNHTTPFRFRLDKSENYYFICTVAATCSAGQKLAIT 122
           A +H+VAGVTKE YDNC T++P F+N T+PF F LDK ++Y+FICT+   CSAGQKLAIT
Sbjct: 64  AGAHDVAGVTKEGYDNCITTDPIFLNTTSPFSFTLDKLDDYFFICTIPGHCSAGQKLAIT 123

Query: 123 NIVKQRSST-----------VTPSPP---------------------PPPSSSASTLASV 167
           N+ +    T            TP+PP                     PPP +SA+++   
Sbjct: 124 NLQQSPPPTSPSTPPVPGNEPTPTPPASPDSPPISNEMPPPVPVIAAPPPPNSATSIVGS 183

[Close]

BLAST of Lag0034317 vs. ExPASy Swiss-Prot
Match: P42849 (Umecyanin OS=Armoracia rusticana OX=3704 PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 8.8e-18
Identity = 46/102 (45.10%), Postives = 62/102 (60.78%), Query Frame = 0


Query: 24  EINVGGATTWRIPSDPNFYSSWVSRETIALHDILVFRFAADSHNVAGVTKEAYDNCDTSN 83
           + +VGG   W+ PSDP FY +W + +T  + D L F FAA  H+VA VTK+A+DNC   N
Sbjct: 2   DYDVGGDMEWKRPSDPKFYITWATGKTFRVGDELEFDFAAGMHDVAVVTKDAFDNCKKEN 61

Query: 84  PKFVNHTTPFRFRLDKSENYYFICTVAATCSAGQKLAITNIV 126
           P     T P +  L+ +   Y+ICTV   C  GQKL+I N+V
Sbjct: 62  PISHMTTPPVKIMLNTTGPQYYICTVGDHCRVGQKLSI-NVV 102

[Close]

BLAST of Lag0034317 vs. ExPASy Swiss-Prot
Match: P29602 (Cucumber peeling cupredoxin OS=Cucumis sativus OX=3659 PE=1 SV=3)

HSP 1 Score: 91.3 bits (225), Expect = 1.1e-17
Identity = 53/123 (43.09%), Postives = 76/123 (61.79%), Query Frame = 0


Query: 27  VGGATTWRIPSDPNFYSSWVSRETIALHDILVFRFAADSHNVAGV-TKEAYDNCD-TSNP 86
           VG  T W +PS PNFYS W + +T  + D L F F A++HNV  + TK+++D C+  ++ 
Sbjct: 7   VGDNTGWSVPSSPNFYSQWAAGKTFRVGDSLQFNFPANAHNVHEMETKQSFDACNFVNSD 66

Query: 87  KFVNHTTPFRFRLDKSENYYFICTVAATCSAGQKLAITNIVKQRSSTVTPSPPPPPSSSA 146
             V  T+P   RLD+   +YF+CTV   CS GQKL+I N+V   ++  T S PPP SS  
Sbjct: 67  NDVERTSPVIERLDELGMHYFVCTVGTHCSNGQKLSI-NVV---AANATVSMPPPSSSPP 125

Query: 147 STL 148
           S++
Sbjct: 127 SSV 125

[Close]

BLAST of Lag0034317 vs. ExPASy Swiss-Prot
Match: Q07488 (Blue copper protein OS=Arabidopsis thaliana OX=3702 GN=BCB PE=2 SV=2)

HSP 1 Score: 87.0 bits (214), Expect = 2.2e-16
Identity = 56/145 (38.62%), Postives = 79/145 (54.48%), Query Frame = 0


Query: 6   FFVVVVAATTVLPAAVAVEINVGGATTWRIPSDPNFYSSWVSRETIALHDILVFRFAADS 65
           F V+V AA  V     A + +VG  T W  P DP FY++W + +T  + D L F FAA  
Sbjct: 10  FLVLVFAAVVVF----AEDYDVGDDTEWTRPMDPEFYTTWATGKTFRVGDELEFDFAAGR 69

Query: 66  HNVAGVTKEAYDNCDTSNPKFVNHTT--PFRFRLDKSENYYFICTVAATCSAGQKLAITN 125
           H+VA V++ A++NC+   P  ++H T  P +  L+ +   YFICTV   C  GQKL+IT 
Sbjct: 70  HDVAVVSEAAFENCEKEKP--ISHMTVPPVKIMLNTTGPQYFICTVGDHCRFGQKLSITV 129

Query: 126 IVKQRSSTVTP--SPPPPPSSSAST 147
           +    +   TP     P P S+ ST
Sbjct: 130 VAAGATGGATPGAGATPAPGSTPST 148

[Close]

BLAST of Lag0034317 vs. ExPASy Swiss-Prot
Match: Q9SK27 (Early nodulin-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At2g25060 PE=2 SV=2)

HSP 1 Score: 74.3 bits (181), Expect = 1.5e-12
Identity = 46/144 (31.94%), Postives = 78/144 (54.17%), Query Frame = 0


Query: 8   VVVVAATTVLPAAVAVEINVGGAT-TWRIPSDPNF-YSSWVSRETIALHDILVFRFAADS 67
           V + +   +   A A E+ VGG +  W+IP   ++ ++ W  +    + D +VFR+ +  
Sbjct: 14  VAIFSLIFLFSLAAANEVTVGGKSGDWKIPPSSSYSFTEWAQKARFKVGDFIVFRYESGK 73

Query: 68  HNVAGVTKEAYDNCDTSNPKFVNHTT-PFRFRLDKSENYYFICTVAATCSAGQKLAITNI 127
            +V  VTKEAY++C+T+NP   N+T    + +LD+S  +YFI      C  GQKL++  +
Sbjct: 74  DSVLEVTKEAYNSCNTTNP-LANYTDGETKVKLDRSGPFYFISGANGHCEKGQKLSLV-V 133

Query: 128 VKQRSSTVTPSPPPPPSSSASTLA 149
           +  R S ++P+P P        LA
Sbjct: 134 ISPRHSVISPAPSPVEFEDGPALA 155

[Close]

BLAST of Lag0034317 vs. ExPASy Swiss-Prot
Match: Q8LC95 (Early nodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g25090 PE=2 SV=2)

HSP 1 Score: 60.5 bits (145), Expect = 2.2e-08
Identity = 52/183 (28.42%), Postives = 89/183 (48.63%), Query Frame = 0


Query: 3   IGIFFVVVVAATTVLPAAVAVEINVGGAT-TWRIPSDPN-FYSSWVSRETIALHDILVFR 62
           +  FF++    T ++    + EI VGG T +W+IPS P+   + W       + D LV++
Sbjct: 7   VATFFLIFFLLTNLV---CSKEIIVGGKTSSWKIPSSPSESLNKWAESLRFRVGDTLVWK 66

Query: 63  FAADSHNVAGVTKEAYDNCDTSNPKFVNHTTPFRFRLDKSENYYFICTVAATCSAGQKLA 122
           +  +  +V  VTK+AY NC+T+NP         + +L++S  Y+FI    + C  G+KL 
Sbjct: 67  YDEEKDSVLQVTKDAYINCNTTNPAANYSNGDTKVKLERSGPYFFISGSKSNCVEGEKLH 126

Query: 123 ITNIVKQRSST------VTPSPPPPPS------------SSASTLASVTFTVAFIFIIAV 166
           I  +  +   T       +PSP P P+             SAS+L      + F+ ++A+
Sbjct: 127 IVVMSSRGGHTGGFFTGSSPSPAPSPALLGAPTVAPASGGSASSLTRQVGVLGFVGLLAI 186

The following BLAST results are available for this feature:

BLAST of Lag0034317 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038878561.1	1.1e-33	50.83	umecyanin-like [Benincasa hispida]	[more]
XP_008443977.1	2.1e-32	48.13	PREDICTED: umecyanin-like [Cucumis melo] >KAA0050151.1 umecyanin-like [Cucumis m...	[more]
XP_008444048.1	3.5e-32	54.86	PREDICTED: umecyanin-like [Cucumis melo]	[more]
KAA0050152.1	6.0e-32	52.60	uncharacterized protein E6C27_scaffold675G001830 [Cucumis melo var. makuwa] >TYK...	[more]
XP_004150037.1	1.9e-30	46.43	cucumber peeling cupredoxin-like [Cucumis sativus] >KGN65338.1 hypothetical prot...	[more]

BLAST of Lag0034317 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P42849	8.8e-18	45.10	Umecyanin OS=Armoracia rusticana OX=3704 PE=1 SV=1	[more]
P29602	1.1e-17	43.09	Cucumber peeling cupredoxin OS=Cucumis sativus OX=3659 PE=1 SV=3	[more]
Q07488	2.2e-16	38.62	Blue copper protein OS=Arabidopsis thaliana OX=3702 GN=BCB PE=2 SV=2	[more]
Q9SK27	1.5e-12	31.94	Early nodulin-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At2g25060 PE=2 S...	[more]
Q8LC95	2.2e-08	28.42	Early nodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g25090 PE=2 S...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003245	Phytocyanin domain	PFAM	PF02298	Cu_bind_like	coord: 33..117 e-value: 3.3E-16 score: 59.1
IPR003245	Phytocyanin domain	PROSITE	PS51485	PHYTOCYANIN	coord: 23..125 score: 30.058073
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 21..125 e-value: 5.2E-29 score: 102.6
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 22..122
IPR039391	Phytocyanin	PANTHER	PTHR33021	BLUE COPPER PROTEIN	coord: 6..154
None	No IPR available	PANTHER	PTHR33021:SF302	BLUE COPPER PROTEIN	coord: 6..154

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0034317.1	Lag0034317.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0046658	anchored component of plasma membrane
molecular_function	GO:0009055	electron transfer activity