Lag0034306 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0034306
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionBeta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
Locationchr3: 6219266 .. 6220387 (+)
RNA-Seq ExpressionLag0034306
SyntenyLag0034306
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTACAAGATCTCTTCGGGTCAATTCTAGACGCATACGACCTAAATATCTTCTTTTTATGCTTCTTTTGCTTGTTCCGATCAGTGTTATTACCATTTTCAATTATGGCCAAAAAATTTCTTATTTCTTCCGCCCACTTTGGGACAGTCCTCCACACCCTTTTGTACGCCTACCACACTATTATGCAGAAAATGTGTCTATGGAACACCTTTGTCGCCTCCATGGCTGGTCTTTGCGATCCAAACCTCGTCGTGTTTTTGATGCCATCATCTTCAGCAATGAGCTAGATTTGCTTGACATCAGATGGCGGGAGCTTTACCCATATGTCTGGAAGTTTGTAATCCTGGAGTCACATACCACTTTTACTGGCATACGCAAGCCATTACTATTTCAAACAAATCGAGATAGGTTTGCCTTTGCAGAGAATAAGACTGTCCATGATGTGTTTTCTGGACAAGTTGCATCTCATGGGTCGCATAGAAACCCATTTGACCTTGAATCCCAGCAGCGTGTAGCTATGAATGGACTACTTCAGCGTGCAGGGATTTCAAATGGCGATCTTTTGATCATGTCGGATACCGATGAGATACCGAGCCCACATACGGTGGAACTACTGCAATGGTGTGATGGGGTGCCTCCTGTAGTCCATCTCGAAATGAGGAACTACATGTATTCTTTTGAGTTCCCTGTGGACTACAGCAGTTGGCGAGCCACGATTCACATCTATGGTCCGCATACTCACTATAGGCATTCTCGCCAAACCGACCTTATATTTTCCGATGCAGGATGGCATTGCAGCTTTTGCTTTAGGAACATTCAAGACTTTGCGTTCAAGATGACAGCTTATAGTCATGCAGACCGTGTAAGGAAGCGAGATTTCTTGAATTACTCAAGGATACAGAAGCTCATTTGTCAAGGAGATGATCTCTTTGACATGCTTCCCGAAGAATATACCTTCCAGGAGTTGATTAAGAAGATGGGATCAATACCGCGTTCTTCATCGGCCGTTCACCTTCCTGCATACTTGATAAAGAATGCAGACAAGTTCAAATTCCTTCTTCCTGGAGGCTGTGTAAGAACCCCTGAAGGCAGCATCCCAGGGTTGAATAAGCAACCGTAG

mRNA sequence

ATGGCTACAAGATCTCTTCGGGTCAATTCTAGACGCATACGACCTAAATATCTTCTTTTTATGCTTCTTTTGCTTGTTCCGATCAGTGTTATTACCATTTTCAATTATGGCCAAAAAATTTCTTATTTCTTCCGCCCACTTTGGGACAGTCCTCCACACCCTTTTGTACGCCTACCACACTATTATGCAGAAAATGTGTCTATGGAACACCTTTGTCGCCTCCATGGCTGGTCTTTGCGATCCAAACCTCGTCGTGTTTTTGATGCCATCATCTTCAGCAATGAGCTAGATTTGCTTGACATCAGATGGCGGGAGCTTTACCCATATGTCTGGAAGTTTGTAATCCTGGAGTCACATACCACTTTTACTGGCATACGCAAGCCATTACTATTTCAAACAAATCGAGATAGGTTTGCCTTTGCAGAGAATAAGACTGTCCATGATGTGTTTTCTGGACAAGTTGCATCTCATGGGTCGCATAGAAACCCATTTGACCTTGAATCCCAGCAGCGTGTAGCTATGAATGGACTACTTCAGCGTGCAGGGATTTCAAATGGCGATCTTTTGATCATGTCGGATACCGATGAGATACCGAGCCCACATACGGTGGAACTACTGCAATGGTGTGATGGGGTGCCTCCTGTAGTCCATCTCGAAATGAGGAACTACATGTATTCTTTTGAGTTCCCTGTGGACTACAGCAGTTGGCGAGCCACGATTCACATCTATGGTCCGCATACTCACTATAGGCATTCTCGCCAAACCGACCTTATATTTTCCGATGCAGGATGGCATTGCAGCTTTTGCTTTAGGAACATTCAAGACTTTGCGTTCAAGATGACAGCTTATAGTCATGCAGACCGTGTAAGGAAGCGAGATTTCTTGAATTACTCAAGGATACAGAAGCTCATTTGTCAAGGAGATGATCTCTTTGACATGCTTCCCGAAGAATATACCTTCCAGGAGTTGATTAAGAAGATGGGATCAATACCGCGTTCTTCATCGGCCGTTCACCTTCCTGCATACTTGATAAAGAATGCAGACAAGTTCAAATTCCTTCTTCCTGGAGGCTGTGTAAGAACCCCTGAAGGCAGCATCCCAGGGTTGAATAAGCAACCGTAG

Coding sequence (CDS)

ATGGCTACAAGATCTCTTCGGGTCAATTCTAGACGCATACGACCTAAATATCTTCTTTTTATGCTTCTTTTGCTTGTTCCGATCAGTGTTATTACCATTTTCAATTATGGCCAAAAAATTTCTTATTTCTTCCGCCCACTTTGGGACAGTCCTCCACACCCTTTTGTACGCCTACCACACTATTATGCAGAAAATGTGTCTATGGAACACCTTTGTCGCCTCCATGGCTGGTCTTTGCGATCCAAACCTCGTCGTGTTTTTGATGCCATCATCTTCAGCAATGAGCTAGATTTGCTTGACATCAGATGGCGGGAGCTTTACCCATATGTCTGGAAGTTTGTAATCCTGGAGTCACATACCACTTTTACTGGCATACGCAAGCCATTACTATTTCAAACAAATCGAGATAGGTTTGCCTTTGCAGAGAATAAGACTGTCCATGATGTGTTTTCTGGACAAGTTGCATCTCATGGGTCGCATAGAAACCCATTTGACCTTGAATCCCAGCAGCGTGTAGCTATGAATGGACTACTTCAGCGTGCAGGGATTTCAAATGGCGATCTTTTGATCATGTCGGATACCGATGAGATACCGAGCCCACATACGGTGGAACTACTGCAATGGTGTGATGGGGTGCCTCCTGTAGTCCATCTCGAAATGAGGAACTACATGTATTCTTTTGAGTTCCCTGTGGACTACAGCAGTTGGCGAGCCACGATTCACATCTATGGTCCGCATACTCACTATAGGCATTCTCGCCAAACCGACCTTATATTTTCCGATGCAGGATGGCATTGCAGCTTTTGCTTTAGGAACATTCAAGACTTTGCGTTCAAGATGACAGCTTATAGTCATGCAGACCGTGTAAGGAAGCGAGATTTCTTGAATTACTCAAGGATACAGAAGCTCATTTGTCAAGGAGATGATCTCTTTGACATGCTTCCCGAAGAATATACCTTCCAGGAGTTGATTAAGAAGATGGGATCAATACCGCGTTCTTCATCGGCCGTTCACCTTCCTGCATACTTGATAAAGAATGCAGACAAGTTCAAATTCCTTCTTCCTGGAGGCTGTGTAAGAACCCCTGAAGGCAGCATCCCAGGGTTGAATAAGCAACCGTAG

Protein sequence

MATRSLRVNSRRIRPKYLLFMLLLLVPISVITIFNYGQKISYFFRPLWDSPPHPFVRLPHYYAENVSMEHLCRLHGWSLRSKPRRVFDAIIFSNELDLLDIRWRELYPYVWKFVILESHTTFTGIRKPLLFQTNRDRFAFAENKTVHDVFSGQVASHGSHRNPFDLESQQRVAMNGLLQRAGISNGDLLIMSDTDEIPSPHTVELLQWCDGVPPVVHLEMRNYMYSFEFPVDYSSWRATIHIYGPHTHYRHSRQTDLIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVRKRDFLNYSRIQKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLIKNADKFKFLLPGGCVRTPEGSIPGLNKQP
Homology
BLAST of Lag0034306 vs. NCBI nr
Match: XP_011656373.1 (beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase [Cucumis sativus] >XP_011656380.1 beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase [Cucumis sativus] >XP_031735984.1 beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like [Cucumis sativus] >XP_031735985.1 beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like [Cucumis sativus] >KAE8637249.1 hypothetical protein CSA_007712 [Cucumis sativus] >KGN65343.1 hypothetical protein Csa_019779 [Cucumis sativus])

HSP 1 Score: 737.6 bits (1903), Expect = 5.1e-209
Identity = 345/373 (92.49%), Postives = 364/373 (97.59%), Query Frame = 0

Query: 1   MATRSLRVNSRRIRPKYLLFMLLLLVPISVITIFNYGQKISYFFRPLWDSPPHPFVRLPH 60
           MATRSLRVNSRR+RPKYLLFMLL+LVPISVITIFNYGQKISYFFRPLWD+PP PFVRLPH
Sbjct: 1   MATRSLRVNSRRLRPKYLLFMLLMLVPISVITIFNYGQKISYFFRPLWDNPPRPFVRLPH 60

Query: 61  YYAENVSMEHLCRLHGWSLRSKPRRVFDAIIFSNELDLLDIRWRELYPYVWKFVILESHT 120
           YYAENVSMEHLCRLHGWSLRS+PRRVFDAIIFSNELDLL+IRW+ELYPYVWKFVILESHT
Sbjct: 61  YYAENVSMEHLCRLHGWSLRSEPRRVFDAIIFSNELDLLEIRWQELYPYVWKFVILESHT 120

Query: 121 TFTGIRKPLLFQTNRDRFAFAENKTVHDVFSGQVASHGSHRNPFDLESQQRVAMNGLLQR 180
           TFTGI+KPLLF  NR RFAFAENKTVHDVFSG++A HG HRNPFDLESQQRVAMNGLLQR
Sbjct: 121 TFTGIQKPLLFNANRARFAFAENKTVHDVFSGKIAPHGLHRNPFDLESQQRVAMNGLLQR 180

Query: 181 AGISNGDLLIMSDTDEIPSPHTVELLQWCDGVPPVVHLEMRNYMYSFEFPVDYSSWRATI 240
           AGISNGDLLIMSDTDEIPSPHTV+LLQWCD VPP+VHLEMRNYMYSFEFPVDYSSWRATI
Sbjct: 181 AGISNGDLLIMSDTDEIPSPHTVKLLQWCDDVPPIVHLEMRNYMYSFEFPVDYSSWRATI 240

Query: 241 HIYGPHTHYRHSRQTDLIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVRKRDFLNYSRI 300
           HIYGPHTHYRHSRQ++LIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVR+RDFLNYSRI
Sbjct: 241 HIYGPHTHYRHSRQSELIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVRRRDFLNYSRI 300

Query: 301 QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLIKNADKFKFLLPGGCVR 360
           QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLI+NADKF+FLLPGGC+R
Sbjct: 301 QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLIENADKFRFLLPGGCIR 360

Query: 361 TPEGSIPGLNKQP 374
           T E +IPGLN+QP
Sbjct: 361 TSEDNIPGLNRQP 373

BLAST of Lag0034306 vs. NCBI nr
Match: XP_008443970.1 (PREDICTED: beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase [Cucumis melo] >XP_008443971.1 PREDICTED: beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase [Cucumis melo] >XP_008443972.1 PREDICTED: beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase [Cucumis melo] >XP_016899825.1 PREDICTED: beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase [Cucumis melo] >KAA0050138.1 beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase [Cucumis melo var. makuwa] >TYK06393.1 beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 734.6 bits (1895), Expect = 4.3e-208
Identity = 345/373 (92.49%), Postives = 362/373 (97.05%), Query Frame = 0

Query: 1   MATRSLRVNSRRIRPKYLLFMLLLLVPISVITIFNYGQKISYFFRPLWDSPPHPFVRLPH 60
           MATRSLRVNSRR+RPKYLLFMLL+LVPISVITIFNYGQKISYFFRPLWD PP PFVRLPH
Sbjct: 1   MATRSLRVNSRRLRPKYLLFMLLMLVPISVITIFNYGQKISYFFRPLWDKPPRPFVRLPH 60

Query: 61  YYAENVSMEHLCRLHGWSLRSKPRRVFDAIIFSNELDLLDIRWRELYPYVWKFVILESHT 120
           YYAENVSMEHLCRLHGWSLRS+PRRVFDAIIFSNELDLL+IRWRELYPYVWKFVILESHT
Sbjct: 61  YYAENVSMEHLCRLHGWSLRSEPRRVFDAIIFSNELDLLEIRWRELYPYVWKFVILESHT 120

Query: 121 TFTGIRKPLLFQTNRDRFAFAENKTVHDVFSGQVASHGSHRNPFDLESQQRVAMNGLLQR 180
           TFTGI+KPLLF  NR RFAFAENKTVHDVFSG++A  GSHRNPFDLES QRVAMNGLL+R
Sbjct: 121 TFTGIQKPLLFDANRARFAFAENKTVHDVFSGRIAPRGSHRNPFDLESHQRVAMNGLLKR 180

Query: 181 AGISNGDLLIMSDTDEIPSPHTVELLQWCDGVPPVVHLEMRNYMYSFEFPVDYSSWRATI 240
           AGISNGDLLIMSDTDEIPSPHTV+LLQWCD VPP+VHLEMRNYMYSFEFPVDYSSWRATI
Sbjct: 181 AGISNGDLLIMSDTDEIPSPHTVKLLQWCDDVPPIVHLEMRNYMYSFEFPVDYSSWRATI 240

Query: 241 HIYGPHTHYRHSRQTDLIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVRKRDFLNYSRI 300
           HIYGPHTHYRHSRQ++LIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVR+RDFLNYSRI
Sbjct: 241 HIYGPHTHYRHSRQSELIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVRRRDFLNYSRI 300

Query: 301 QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLIKNADKFKFLLPGGCVR 360
           QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLI+NADKF+FLLPGGCVR
Sbjct: 301 QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLIENADKFRFLLPGGCVR 360

Query: 361 TPEGSIPGLNKQP 374
           T E +IPGLN+QP
Sbjct: 361 TSEENIPGLNRQP 373

BLAST of Lag0034306 vs. NCBI nr
Match: XP_022960764.1 (beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 723.4 bits (1866), Expect = 1.0e-204
Identity = 341/373 (91.42%), Postives = 361/373 (96.78%), Query Frame = 0

Query: 1   MATRSLRVNSRRIRPKYLLFMLLLLVPISVITIFNYGQKISYFFRPLWDSPPHPFVRLPH 60
           MA RSLRVNSRRIRPKYLLFMLL+LVPISVITIFNYGQKISYFFRPLWD+PP PFVRLPH
Sbjct: 1   MAIRSLRVNSRRIRPKYLLFMLLILVPISVITIFNYGQKISYFFRPLWDNPPRPFVRLPH 60

Query: 61  YYAENVSMEHLCRLHGWSLRSKPRRVFDAIIFSNELDLLDIRWRELYPYVWKFVILESHT 120
           YYAENVSMEHLCRLHGWSLRS+PRRVFDAIIFSNELDLL+IRWRELYPYVWKFVILESHT
Sbjct: 61  YYAENVSMEHLCRLHGWSLRSEPRRVFDAIIFSNELDLLEIRWRELYPYVWKFVILESHT 120

Query: 121 TFTGIRKPLLFQTNRDRFAFAENKTVHDVFSGQVASHGSHRNPFDLESQQRVAMNGLLQR 180
           TFTGIRKPL+F  NR RF+FA+NKTVHDVFSG+VAS GSHRNPFDLESQQRVAMN LL+R
Sbjct: 121 TFTGIRKPLVFDANRARFSFAKNKTVHDVFSGKVASPGSHRNPFDLESQQRVAMNRLLRR 180

Query: 181 AGISNGDLLIMSDTDEIPSPHTVELLQWCDGVPPVVHLEMRNYMYSFEFPVDYSSWRATI 240
           AGISNGDLLIMSDTDEIPSPHTV+LLQWCD VP +VHLEMRNYMYSFEFPVDYSSWRA++
Sbjct: 181 AGISNGDLLIMSDTDEIPSPHTVKLLQWCDEVPSIVHLEMRNYMYSFEFPVDYSSWRASV 240

Query: 241 HIYGPHTHYRHSRQTDLIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVRKRDFLNYSRI 300
           HIYGP+THYRHSRQT+LIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVR+RDFLNY+RI
Sbjct: 241 HIYGPYTHYRHSRQTNLIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVRRRDFLNYARI 300

Query: 301 QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLIKNADKFKFLLPGGCVR 360
           QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLI+NADKF+FLLPGGCVR
Sbjct: 301 QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLIENADKFRFLLPGGCVR 360

Query: 361 TPEGSIPGLNKQP 374
           T   SIPGLN+QP
Sbjct: 361 TSANSIPGLNRQP 373

BLAST of Lag0034306 vs. NCBI nr
Match: XP_023515748.1 (beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 721.8 bits (1862), Expect = 2.9e-204
Identity = 341/373 (91.42%), Postives = 360/373 (96.51%), Query Frame = 0

Query: 1   MATRSLRVNSRRIRPKYLLFMLLLLVPISVITIFNYGQKISYFFRPLWDSPPHPFVRLPH 60
           MA RSLRVNSRRIRPKYLLFMLL+LVPISVITIFNYGQKISYFFRPLWD+PP PFVRLPH
Sbjct: 1   MAIRSLRVNSRRIRPKYLLFMLLILVPISVITIFNYGQKISYFFRPLWDNPPRPFVRLPH 60

Query: 61  YYAENVSMEHLCRLHGWSLRSKPRRVFDAIIFSNELDLLDIRWRELYPYVWKFVILESHT 120
           YYAENVSMEHLCRLHGWSLRS+PRRVFDAIIFSNELDLL+IRWRELYPYVWKFVILESHT
Sbjct: 61  YYAENVSMEHLCRLHGWSLRSEPRRVFDAIIFSNELDLLEIRWRELYPYVWKFVILESHT 120

Query: 121 TFTGIRKPLLFQTNRDRFAFAENKTVHDVFSGQVASHGSHRNPFDLESQQRVAMNGLLQR 180
           TFTGIRKPL+F  NR RF+FAENKTVHDVFSG+VAS GSHRNPFDLESQQRVAMN LL+R
Sbjct: 121 TFTGIRKPLVFDANRARFSFAENKTVHDVFSGKVASPGSHRNPFDLESQQRVAMNRLLRR 180

Query: 181 AGISNGDLLIMSDTDEIPSPHTVELLQWCDGVPPVVHLEMRNYMYSFEFPVDYSSWRATI 240
           AGISNGDLLIMSDTDEIPSPHTV+LLQWCD VP +VHLEMRNYMYSFEFPVDYSSWRA++
Sbjct: 181 AGISNGDLLIMSDTDEIPSPHTVKLLQWCDEVPSIVHLEMRNYMYSFEFPVDYSSWRASV 240

Query: 241 HIYGPHTHYRHSRQTDLIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVRKRDFLNYSRI 300
           HIYG +THYRHSRQT+LIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVR+RDFLNY+RI
Sbjct: 241 HIYGSYTHYRHSRQTNLIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVRRRDFLNYARI 300

Query: 301 QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLIKNADKFKFLLPGGCVR 360
           QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLI+NADKF+FLLPGGCVR
Sbjct: 301 QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLIENADKFRFLLPGGCVR 360

Query: 361 TPEGSIPGLNKQP 374
           T   SIPGLN+QP
Sbjct: 361 TSANSIPGLNRQP 373

BLAST of Lag0034306 vs. NCBI nr
Match: KAG6589835.1 (Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 721.8 bits (1862), Expect = 2.9e-204
Identity = 340/373 (91.15%), Postives = 360/373 (96.51%), Query Frame = 0

Query: 1   MATRSLRVNSRRIRPKYLLFMLLLLVPISVITIFNYGQKISYFFRPLWDSPPHPFVRLPH 60
           MA RSLRVNSRRIRPKYLLFMLL+ VPISVITIFNYGQKISYFFRPLWD+PP PFVRLPH
Sbjct: 1   MAIRSLRVNSRRIRPKYLLFMLLIFVPISVITIFNYGQKISYFFRPLWDNPPRPFVRLPH 60

Query: 61  YYAENVSMEHLCRLHGWSLRSKPRRVFDAIIFSNELDLLDIRWRELYPYVWKFVILESHT 120
           YYAENVSMEHLCRLHGWSLRS+PRRVFDAIIFSNELDLL+IRWRELYPYVWKFVILESHT
Sbjct: 61  YYAENVSMEHLCRLHGWSLRSEPRRVFDAIIFSNELDLLEIRWRELYPYVWKFVILESHT 120

Query: 121 TFTGIRKPLLFQTNRDRFAFAENKTVHDVFSGQVASHGSHRNPFDLESQQRVAMNGLLQR 180
           TFTGIRKPL+F  NR RF+FA+NKTVHDVFSG+VAS GSHRNPFDLESQQRVAMN LL+R
Sbjct: 121 TFTGIRKPLVFDANRARFSFAKNKTVHDVFSGKVASPGSHRNPFDLESQQRVAMNRLLRR 180

Query: 181 AGISNGDLLIMSDTDEIPSPHTVELLQWCDGVPPVVHLEMRNYMYSFEFPVDYSSWRATI 240
           AGISNGDLLIMSDTDEIPSPHTV+LLQWCD VP +VHLEMRNYMYSFEFPVDYSSWRA++
Sbjct: 181 AGISNGDLLIMSDTDEIPSPHTVKLLQWCDEVPSIVHLEMRNYMYSFEFPVDYSSWRASV 240

Query: 241 HIYGPHTHYRHSRQTDLIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVRKRDFLNYSRI 300
           HIYGP+THYRHSRQT+LIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVR+RDFLNY+RI
Sbjct: 241 HIYGPYTHYRHSRQTNLIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVRRRDFLNYARI 300

Query: 301 QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLIKNADKFKFLLPGGCVR 360
           QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLI+NADKF+FLLPGGCVR
Sbjct: 301 QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLIENADKFRFLLPGGCVR 360

Query: 361 TPEGSIPGLNKQP 374
           T   SIPGLN+QP
Sbjct: 361 TSANSIPGLNRQP 373

BLAST of Lag0034306 vs. ExPASy Swiss-Prot
Match: Q02527 (Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase OS=Rattus norvegicus OX=10116 GN=Mgat3 PE=1 SV=2)

HSP 1 Score: 89.4 bits (220), Expect = 9.6e-17
Identity = 88/327 (26.91%), Postives = 140/327 (42.81%), Query Frame = 0

Query: 80  RSKPRRVFDAIIFSNELDLLDIRWRELYPYVWKFVILESHTTFTGIRKPLLFQ--TNRDR 139
           R  PRRV +AI  ++E DLLD+R+ EL   V  FV+ ES+ T  G  +PL F+       
Sbjct: 206 REVPRRVINAININHEFDLLDVRFHELGDVVDAFVVCESNFTAYGEPRPLKFREMLTNGT 265

Query: 140 FAFAENKTVHDVFSGQVASHGSHRNPFDLESQQR--VAMNGLLQRAGISNGDLLIMSDTD 199
           F +  +K ++ VF       G  ++ +  +   R  +  +G+ +   +   D+ I+ D D
Sbjct: 266 FEYIRHKVLY-VFLDHFPP-GGRQDGWIADDYLRTFLTQDGVSRLRNLRPDDVFIIDDAD 325

Query: 200 EIPSPHTVELLQWCDGVPPVVHLEMRNYMYSF---------------------EFPVDYS 259
           EIP+   V  L+  DG        MR  +Y F                      + +D  
Sbjct: 326 EIPARDGVLFLKLYDGWTEPFAFHMRKSLYGFFWKQPGTLEVVSGCTIDMLQAVYGLDGI 385

Query: 260 SWRATIHIYGPHTHYRHSRQTDLI--------FSDAGWHCSFCFRNIQDFAFKMTAYSHA 319
             R   +   P+     +R   ++           AGWHCS+CF   +   FK+ +  + 
Sbjct: 386 RLRRRQYYTMPNFRQYENRTGHILVQWSLGSPLHFAGWHCSWCF-TPEGIYFKLVSAQNG 445

Query: 320 DRVRKRDF-----LNYSRIQKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPA 369
           D  R  D+     LNY  I+ LI  G   FD   +EY   +           S  ++ P 
Sbjct: 446 DFPRWGDYEDKRDLNY--IRSLIRTG-GWFDGTQQEYPPAD----------PSEHMYAPK 505

BLAST of Lag0034306 vs. ExPASy Swiss-Prot
Match: Q10470 (Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase OS=Mus musculus OX=10090 GN=Mgat3 PE=1 SV=2)

HSP 1 Score: 88.2 bits (217), Expect = 2.1e-16
Identity = 87/327 (26.61%), Postives = 140/327 (42.81%), Query Frame = 0

Query: 80  RSKPRRVFDAIIFSNELDLLDIRWRELYPYVWKFVILESHTTFTGIRKPLLFQ--TNRDR 139
           R  PRRV +AI  ++E DLLD+R+ EL   V  FV+ +S+ T  G  +PL F+       
Sbjct: 206 REVPRRVINAININHEFDLLDVRFHELGDVVDAFVVCDSNFTAYGEPRPLKFREMLTNGT 265

Query: 140 FAFAENKTVHDVFSGQVASHGSHRNPFDLESQQR--VAMNGLLQRAGISNGDLLIMSDTD 199
           F +  +K ++ VF       G  ++ +  +   R  +  +G+ +   +   D+ I+ D D
Sbjct: 266 FEYIRHKVLY-VFLDHFPP-GGRQDGWIADDYLRTFLTQDGVSRLRNLRPDDVFIIDDAD 325

Query: 200 EIPSPHTVELLQWCDGVPPVVHLEMRNYMYSF---------------------EFPVDYS 259
           EIP+   V  L+  DG        MR  +Y F                      + +D  
Sbjct: 326 EIPARDGVLFLKLYDGWTEPFAFHMRKSLYGFFWKQPGTLEVVSGCTMDMLQAVYGLDGI 385

Query: 260 SWRATIHIYGPHTHYRHSRQTDLI--------FSDAGWHCSFCFRNIQDFAFKMTAYSHA 319
             R   +   P+     +R   ++           AGWHCS+CF   +   FK+ +  + 
Sbjct: 386 RLRRRQYYTMPNFRQYENRTGHILVQWSLGSPLHFAGWHCSWCF-TPEGIYFKLVSAQNG 445

Query: 320 DRVRKRDF-----LNYSRIQKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPA 369
           D  R  D+     LNY  I+ LI  G   FD   +EY   +           S  ++ P 
Sbjct: 446 DFPRWGDYEDKRDLNY--IRSLIRTG-GWFDGTQQEYPPAD----------PSEHMYAPK 505

BLAST of Lag0034306 vs. ExPASy Swiss-Prot
Match: Q09327 (Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase OS=Homo sapiens OX=9606 GN=MGAT3 PE=1 SV=3)

HSP 1 Score: 86.7 bits (213), Expect = 6.2e-16
Identity = 92/342 (26.90%), Postives = 140/342 (40.94%), Query Frame = 0

Query: 80  RSKPRRVFDAIIFSNELDLLDIRWRELYPYVWKFVILESHTTFTGIRKPLLFQ--TNRDR 139
           R  PRRV +AI  ++E DLLD+R+ EL   V  FV+ ES+ T  G  +PL F+       
Sbjct: 202 REVPRRVINAINVNHEFDLLDVRFHELGDVVDAFVVCESNFTAYGEPRPLKFREMLTNGT 261

Query: 140 FAFAENKTVHDVFSGQVASHGSHRNPFDLESQQR--VAMNGLLQRAGISNGDLLIMSDTD 199
           F +  +K ++ VF       G  ++ +  +   R  +  +G+ +   +   D+ I+ D D
Sbjct: 262 FEYIRHKVLY-VFLDHFPP-GGRQDGWIADDYLRTFLTQDGVSRLRNLRPDDVFIIDDAD 321

Query: 200 EIPSPHTVELLQWCDGVPPVVHLEMRNYMYSF---------------------EFPVDYS 259
           EIP+   V  L+  DG        MR  +Y F                      + +D  
Sbjct: 322 EIPARDGVLFLKLYDGWTEPFAFHMRKSLYGFFWKQPGTLEVVSGCTVDMLQAVYGLDGI 381

Query: 260 SWRATIHIYGPHTHYRHSRQTDLI--------FSDAGWHCSFCFRNIQDFAFKMTAYSHA 319
             R   +   P+     +R   ++           AGWHCS+CF   +   FK+ +  + 
Sbjct: 382 RLRRRQYYTMPNFRQYENRTGHILVQWSLGSPLHFAGWHCSWCF-TPEGIYFKLVSAQNG 441

Query: 320 DRVRKRDF-----LNYSRIQKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPA 372
           D  R  D+     LNY  I+ LI  G   FD   +EY   +           S  ++ P 
Sbjct: 442 DFPRWGDYEDKRDLNY--IRGLIRTG-GWFDGTQQEYPPAD----------PSEHMYAPK 501

BLAST of Lag0034306 vs. ExPASy TrEMBL
Match: A0A0A0LU59 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G351960 PE=4 SV=1)

HSP 1 Score: 737.6 bits (1903), Expect = 2.5e-209
Identity = 345/373 (92.49%), Postives = 364/373 (97.59%), Query Frame = 0

Query: 1   MATRSLRVNSRRIRPKYLLFMLLLLVPISVITIFNYGQKISYFFRPLWDSPPHPFVRLPH 60
           MATRSLRVNSRR+RPKYLLFMLL+LVPISVITIFNYGQKISYFFRPLWD+PP PFVRLPH
Sbjct: 1   MATRSLRVNSRRLRPKYLLFMLLMLVPISVITIFNYGQKISYFFRPLWDNPPRPFVRLPH 60

Query: 61  YYAENVSMEHLCRLHGWSLRSKPRRVFDAIIFSNELDLLDIRWRELYPYVWKFVILESHT 120
           YYAENVSMEHLCRLHGWSLRS+PRRVFDAIIFSNELDLL+IRW+ELYPYVWKFVILESHT
Sbjct: 61  YYAENVSMEHLCRLHGWSLRSEPRRVFDAIIFSNELDLLEIRWQELYPYVWKFVILESHT 120

Query: 121 TFTGIRKPLLFQTNRDRFAFAENKTVHDVFSGQVASHGSHRNPFDLESQQRVAMNGLLQR 180
           TFTGI+KPLLF  NR RFAFAENKTVHDVFSG++A HG HRNPFDLESQQRVAMNGLLQR
Sbjct: 121 TFTGIQKPLLFNANRARFAFAENKTVHDVFSGKIAPHGLHRNPFDLESQQRVAMNGLLQR 180

Query: 181 AGISNGDLLIMSDTDEIPSPHTVELLQWCDGVPPVVHLEMRNYMYSFEFPVDYSSWRATI 240
           AGISNGDLLIMSDTDEIPSPHTV+LLQWCD VPP+VHLEMRNYMYSFEFPVDYSSWRATI
Sbjct: 181 AGISNGDLLIMSDTDEIPSPHTVKLLQWCDDVPPIVHLEMRNYMYSFEFPVDYSSWRATI 240

Query: 241 HIYGPHTHYRHSRQTDLIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVRKRDFLNYSRI 300
           HIYGPHTHYRHSRQ++LIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVR+RDFLNYSRI
Sbjct: 241 HIYGPHTHYRHSRQSELIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVRRRDFLNYSRI 300

Query: 301 QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLIKNADKFKFLLPGGCVR 360
           QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLI+NADKF+FLLPGGC+R
Sbjct: 301 QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLIENADKFRFLLPGGCIR 360

Query: 361 TPEGSIPGLNKQP 374
           T E +IPGLN+QP
Sbjct: 361 TSEDNIPGLNRQP 373

BLAST of Lag0034306 vs. ExPASy TrEMBL
Match: A0A1S4DVT1 (beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase OS=Cucumis melo OX=3656 GN=LOC103487429 PE=4 SV=1)

HSP 1 Score: 734.6 bits (1895), Expect = 2.1e-208
Identity = 345/373 (92.49%), Postives = 362/373 (97.05%), Query Frame = 0

Query: 1   MATRSLRVNSRRIRPKYLLFMLLLLVPISVITIFNYGQKISYFFRPLWDSPPHPFVRLPH 60
           MATRSLRVNSRR+RPKYLLFMLL+LVPISVITIFNYGQKISYFFRPLWD PP PFVRLPH
Sbjct: 1   MATRSLRVNSRRLRPKYLLFMLLMLVPISVITIFNYGQKISYFFRPLWDKPPRPFVRLPH 60

Query: 61  YYAENVSMEHLCRLHGWSLRSKPRRVFDAIIFSNELDLLDIRWRELYPYVWKFVILESHT 120
           YYAENVSMEHLCRLHGWSLRS+PRRVFDAIIFSNELDLL+IRWRELYPYVWKFVILESHT
Sbjct: 61  YYAENVSMEHLCRLHGWSLRSEPRRVFDAIIFSNELDLLEIRWRELYPYVWKFVILESHT 120

Query: 121 TFTGIRKPLLFQTNRDRFAFAENKTVHDVFSGQVASHGSHRNPFDLESQQRVAMNGLLQR 180
           TFTGI+KPLLF  NR RFAFAENKTVHDVFSG++A  GSHRNPFDLES QRVAMNGLL+R
Sbjct: 121 TFTGIQKPLLFDANRARFAFAENKTVHDVFSGRIAPRGSHRNPFDLESHQRVAMNGLLKR 180

Query: 181 AGISNGDLLIMSDTDEIPSPHTVELLQWCDGVPPVVHLEMRNYMYSFEFPVDYSSWRATI 240
           AGISNGDLLIMSDTDEIPSPHTV+LLQWCD VPP+VHLEMRNYMYSFEFPVDYSSWRATI
Sbjct: 181 AGISNGDLLIMSDTDEIPSPHTVKLLQWCDDVPPIVHLEMRNYMYSFEFPVDYSSWRATI 240

Query: 241 HIYGPHTHYRHSRQTDLIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVRKRDFLNYSRI 300
           HIYGPHTHYRHSRQ++LIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVR+RDFLNYSRI
Sbjct: 241 HIYGPHTHYRHSRQSELIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVRRRDFLNYSRI 300

Query: 301 QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLIKNADKFKFLLPGGCVR 360
           QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLI+NADKF+FLLPGGCVR
Sbjct: 301 QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLIENADKFRFLLPGGCVR 360

Query: 361 TPEGSIPGLNKQP 374
           T E +IPGLN+QP
Sbjct: 361 TSEENIPGLNRQP 373

BLAST of Lag0034306 vs. ExPASy TrEMBL
Match: A0A5A7U4F4 (Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G00900 PE=4 SV=1)

HSP 1 Score: 734.6 bits (1895), Expect = 2.1e-208
Identity = 345/373 (92.49%), Postives = 362/373 (97.05%), Query Frame = 0

Query: 1   MATRSLRVNSRRIRPKYLLFMLLLLVPISVITIFNYGQKISYFFRPLWDSPPHPFVRLPH 60
           MATRSLRVNSRR+RPKYLLFMLL+LVPISVITIFNYGQKISYFFRPLWD PP PFVRLPH
Sbjct: 1   MATRSLRVNSRRLRPKYLLFMLLMLVPISVITIFNYGQKISYFFRPLWDKPPRPFVRLPH 60

Query: 61  YYAENVSMEHLCRLHGWSLRSKPRRVFDAIIFSNELDLLDIRWRELYPYVWKFVILESHT 120
           YYAENVSMEHLCRLHGWSLRS+PRRVFDAIIFSNELDLL+IRWRELYPYVWKFVILESHT
Sbjct: 61  YYAENVSMEHLCRLHGWSLRSEPRRVFDAIIFSNELDLLEIRWRELYPYVWKFVILESHT 120

Query: 121 TFTGIRKPLLFQTNRDRFAFAENKTVHDVFSGQVASHGSHRNPFDLESQQRVAMNGLLQR 180
           TFTGI+KPLLF  NR RFAFAENKTVHDVFSG++A  GSHRNPFDLES QRVAMNGLL+R
Sbjct: 121 TFTGIQKPLLFDANRARFAFAENKTVHDVFSGRIAPRGSHRNPFDLESHQRVAMNGLLKR 180

Query: 181 AGISNGDLLIMSDTDEIPSPHTVELLQWCDGVPPVVHLEMRNYMYSFEFPVDYSSWRATI 240
           AGISNGDLLIMSDTDEIPSPHTV+LLQWCD VPP+VHLEMRNYMYSFEFPVDYSSWRATI
Sbjct: 181 AGISNGDLLIMSDTDEIPSPHTVKLLQWCDDVPPIVHLEMRNYMYSFEFPVDYSSWRATI 240

Query: 241 HIYGPHTHYRHSRQTDLIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVRKRDFLNYSRI 300
           HIYGPHTHYRHSRQ++LIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVR+RDFLNYSRI
Sbjct: 241 HIYGPHTHYRHSRQSELIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVRRRDFLNYSRI 300

Query: 301 QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLIKNADKFKFLLPGGCVR 360
           QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLI+NADKF+FLLPGGCVR
Sbjct: 301 QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLIENADKFRFLLPGGCVR 360

Query: 361 TPEGSIPGLNKQP 374
           T E +IPGLN+QP
Sbjct: 361 TSEENIPGLNRQP 373

BLAST of Lag0034306 vs. ExPASy TrEMBL
Match: A0A6J1HC23 (beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461467 PE=4 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 4.9e-205
Identity = 341/373 (91.42%), Postives = 361/373 (96.78%), Query Frame = 0

Query: 1   MATRSLRVNSRRIRPKYLLFMLLLLVPISVITIFNYGQKISYFFRPLWDSPPHPFVRLPH 60
           MA RSLRVNSRRIRPKYLLFMLL+LVPISVITIFNYGQKISYFFRPLWD+PP PFVRLPH
Sbjct: 1   MAIRSLRVNSRRIRPKYLLFMLLILVPISVITIFNYGQKISYFFRPLWDNPPRPFVRLPH 60

Query: 61  YYAENVSMEHLCRLHGWSLRSKPRRVFDAIIFSNELDLLDIRWRELYPYVWKFVILESHT 120
           YYAENVSMEHLCRLHGWSLRS+PRRVFDAIIFSNELDLL+IRWRELYPYVWKFVILESHT
Sbjct: 61  YYAENVSMEHLCRLHGWSLRSEPRRVFDAIIFSNELDLLEIRWRELYPYVWKFVILESHT 120

Query: 121 TFTGIRKPLLFQTNRDRFAFAENKTVHDVFSGQVASHGSHRNPFDLESQQRVAMNGLLQR 180
           TFTGIRKPL+F  NR RF+FA+NKTVHDVFSG+VAS GSHRNPFDLESQQRVAMN LL+R
Sbjct: 121 TFTGIRKPLVFDANRARFSFAKNKTVHDVFSGKVASPGSHRNPFDLESQQRVAMNRLLRR 180

Query: 181 AGISNGDLLIMSDTDEIPSPHTVELLQWCDGVPPVVHLEMRNYMYSFEFPVDYSSWRATI 240
           AGISNGDLLIMSDTDEIPSPHTV+LLQWCD VP +VHLEMRNYMYSFEFPVDYSSWRA++
Sbjct: 181 AGISNGDLLIMSDTDEIPSPHTVKLLQWCDEVPSIVHLEMRNYMYSFEFPVDYSSWRASV 240

Query: 241 HIYGPHTHYRHSRQTDLIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVRKRDFLNYSRI 300
           HIYGP+THYRHSRQT+LIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVR+RDFLNY+RI
Sbjct: 241 HIYGPYTHYRHSRQTNLIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVRRRDFLNYARI 300

Query: 301 QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLIKNADKFKFLLPGGCVR 360
           QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLI+NADKF+FLLPGGCVR
Sbjct: 301 QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLIENADKFRFLLPGGCVR 360

Query: 361 TPEGSIPGLNKQP 374
           T   SIPGLN+QP
Sbjct: 361 TSANSIPGLNRQP 373

BLAST of Lag0034306 vs. ExPASy TrEMBL
Match: A0A6J1JHS1 (beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485214 PE=4 SV=1)

HSP 1 Score: 721.1 bits (1860), Expect = 2.4e-204
Identity = 341/373 (91.42%), Postives = 360/373 (96.51%), Query Frame = 0

Query: 1   MATRSLRVNSRRIRPKYLLFMLLLLVPISVITIFNYGQKISYFFRPLWDSPPHPFVRLPH 60
           MA RSLRVNSRRIR KYLLFMLL+LVPISVITIFNYGQKISYFFRPLWD+PP PFVRLPH
Sbjct: 1   MAIRSLRVNSRRIRLKYLLFMLLILVPISVITIFNYGQKISYFFRPLWDNPPRPFVRLPH 60

Query: 61  YYAENVSMEHLCRLHGWSLRSKPRRVFDAIIFSNELDLLDIRWRELYPYVWKFVILESHT 120
           YYAENVSMEHLCRLHGWSLRS+PRRVFDAIIFSNELDLL+IRWRELYPYVWKFVILESHT
Sbjct: 61  YYAENVSMEHLCRLHGWSLRSEPRRVFDAIIFSNELDLLEIRWRELYPYVWKFVILESHT 120

Query: 121 TFTGIRKPLLFQTNRDRFAFAENKTVHDVFSGQVASHGSHRNPFDLESQQRVAMNGLLQR 180
           TFTGIRKPL+F  NR RF+FAENKTVHDVFSG+VAS GSHRNPFDLESQQRVAMN LL+R
Sbjct: 121 TFTGIRKPLVFDANRARFSFAENKTVHDVFSGKVASPGSHRNPFDLESQQRVAMNRLLRR 180

Query: 181 AGISNGDLLIMSDTDEIPSPHTVELLQWCDGVPPVVHLEMRNYMYSFEFPVDYSSWRATI 240
           AGISNGDLLIMSDTDEIPSPHTV+LLQWCD VP +VHLEMRNYMYSFEFPVDYSSWRA++
Sbjct: 181 AGISNGDLLIMSDTDEIPSPHTVKLLQWCDEVPSIVHLEMRNYMYSFEFPVDYSSWRASV 240

Query: 241 HIYGPHTHYRHSRQTDLIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVRKRDFLNYSRI 300
           HIYGP+THYRHSRQT+LIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVR+RDFLNY+RI
Sbjct: 241 HIYGPYTHYRHSRQTNLIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVRRRDFLNYARI 300

Query: 301 QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLIKNADKFKFLLPGGCVR 360
           QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLI+NADKF+FLLPGGCVR
Sbjct: 301 QKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLIENADKFRFLLPGGCVR 360

Query: 361 TPEGSIPGLNKQP 374
           T   SIPGLN+QP
Sbjct: 361 TSANSIPGLNRQP 373

BLAST of Lag0034306 vs. TAIR 10
Match: AT2G13290.1 (beta-1,4-N-acetylglucosaminyltransferase family protein )

HSP 1 Score: 510.8 bits (1314), Expect = 9.5e-145
Identity = 248/365 (67.95%), Postives = 292/365 (80.00%), Query Frame = 0

Query: 1   MATRSLRVNSRRIRPKYLLF-MLLLLVPISVITIFNYGQKISYFFRPLWDSPPHPFVRLP 60
           M  RSL    R+  P  L+F  L++L    ++ I    Q ISY FRPLWD PP PF R+P
Sbjct: 1   MTIRSL----RKPPPVKLVFPTLIILFLTCLLCILTNFQTISYLFRPLWDKPPPPFKRIP 60

Query: 61  HYYAENVSMEHLCRLHGWSLRSKPRRVFDAIIFSNELDLLDIRWRELYPYVWKFVILESH 120
           HYYAENVSM HLC LHGW+ R +PRRVFDAIIFSNELDLL++RWREL PYV KFVILES+
Sbjct: 61  HYYAENVSMGHLCELHGWTPRLEPRRVFDAIIFSNELDLLEVRWRELEPYVSKFVILESN 120

Query: 121 TTFTGIRKPLLFQTNRDRFAFAENKTVHDVFSGQVASHGS-HRNPFDLESQQRVAMNGLL 180
           TTFTGI KPL F +N++RFAFAE K VH VF G+  S G  + +PF LE QQRVAMN LL
Sbjct: 121 TTFTGIPKPLFFDSNKERFAFAEGKIVHGVFPGKKRSTGQPYEDPFLLEGQQRVAMNWLL 180

Query: 181 QRAGISNGDLLIMSDTDEIPSPHTVELLQWCDGVPPVVHLEMRNYMYSFEFPVDYSSWRA 240
           + AG+S+GD +IMSD DEIPSPHTV+ LQWCDG+P V+HLEMR YMYSFEFPVDYSSWRA
Sbjct: 181 REAGVSDGDAVIMSDADEIPSPHTVKFLQWCDGIPDVMHLEMREYMYSFEFPVDYSSWRA 240

Query: 241 TIHIYG-PHTHYRHSRQTDLIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVRKRDFLNY 300
           ++HIY    T YRHSRQTDLI SDAGWHCSFCFR + +F FKM  YSHADRV++++FL+Y
Sbjct: 241 SVHIYSRKWTQYRHSRQTDLILSDAGWHCSFCFRRLNEFVFKMKGYSHADRVKRKEFLDY 300

Query: 301 SRIQKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLIKNADKFKFLLPGG 360
            RIQK IC+G DLFDMLPE+Y+FQELI K+G IP S+SAVHLPA+LI+NA +F+FLLPGG
Sbjct: 301 QRIQKHICKGYDLFDMLPEKYSFQELISKIGPIPPSASAVHLPAFLIQNAARFRFLLPGG 360

Query: 361 CVRTP 363
           C+R P
Sbjct: 361 CLREP 361

BLAST of Lag0034306 vs. TAIR 10
Match: AT2G13290.2 (beta-1,4-N-acetylglucosaminyltransferase family protein )

HSP 1 Score: 510.8 bits (1314), Expect = 9.5e-145
Identity = 248/365 (67.95%), Postives = 292/365 (80.00%), Query Frame = 0

Query: 1   MATRSLRVNSRRIRPKYLLF-MLLLLVPISVITIFNYGQKISYFFRPLWDSPPHPFVRLP 60
           M  RSL    R+  P  L+F  L++L    ++ I    Q ISY FRPLWD PP PF R+P
Sbjct: 1   MTIRSL----RKPPPVKLVFPTLIILFLTCLLCILTNFQTISYLFRPLWDKPPPPFKRIP 60

Query: 61  HYYAENVSMEHLCRLHGWSLRSKPRRVFDAIIFSNELDLLDIRWRELYPYVWKFVILESH 120
           HYYAENVSM HLC LHGW+ R +PRRVFDAIIFSNELDLL++RWREL PYV KFVILES+
Sbjct: 61  HYYAENVSMGHLCELHGWTPRLEPRRVFDAIIFSNELDLLEVRWRELEPYVSKFVILESN 120

Query: 121 TTFTGIRKPLLFQTNRDRFAFAENKTVHDVFSGQVASHGS-HRNPFDLESQQRVAMNGLL 180
           TTFTGI KPL F +N++RFAFAE K VH VF G+  S G  + +PF LE QQRVAMN LL
Sbjct: 121 TTFTGIPKPLFFDSNKERFAFAEGKIVHGVFPGKKRSTGQPYEDPFLLEGQQRVAMNWLL 180

Query: 181 QRAGISNGDLLIMSDTDEIPSPHTVELLQWCDGVPPVVHLEMRNYMYSFEFPVDYSSWRA 240
           + AG+S+GD +IMSD DEIPSPHTV+ LQWCDG+P V+HLEMR YMYSFEFPVDYSSWRA
Sbjct: 181 REAGVSDGDAVIMSDADEIPSPHTVKFLQWCDGIPDVMHLEMREYMYSFEFPVDYSSWRA 240

Query: 241 TIHIYG-PHTHYRHSRQTDLIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVRKRDFLNY 300
           ++HIY    T YRHSRQTDLI SDAGWHCSFCFR + +F FKM  YSHADRV++++FL+Y
Sbjct: 241 SVHIYSRKWTQYRHSRQTDLILSDAGWHCSFCFRRLNEFVFKMKGYSHADRVKRKEFLDY 300

Query: 301 SRIQKLICQGDDLFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLIKNADKFKFLLPGG 360
            RIQK IC+G DLFDMLPE+Y+FQELI K+G IP S+SAVHLPA+LI+NA +F+FLLPGG
Sbjct: 301 QRIQKHICKGYDLFDMLPEKYSFQELISKIGPIPPSASAVHLPAFLIQNAARFRFLLPGG 360

Query: 361 CVRTP 363
           C+R P
Sbjct: 361 CLREP 361

BLAST of Lag0034306 vs. TAIR 10
Match: AT3G27540.1 (beta-1,4-N-acetylglucosaminyltransferase family protein )

HSP 1 Score: 451.1 bits (1159), Expect = 8.9e-127
Identity = 203/340 (59.71%), Postives = 264/340 (77.65%), Query Frame = 0

Query: 22  LLLLVPISVITIFNYGQKISYFFRPLWDSPPHPFVRLPHYYAENVSMEHLCRLHGWSLRS 81
           L +L+P  ++ I+ +GQK +YFFRPLW+SPP PF  +PHYY ENV+ME LC LHGW +R 
Sbjct: 47  LFVLMPFGILAIYLHGQKFTYFFRPLWESPPKPFQTIPHYYNENVTMESLCSLHGWGIRD 106

Query: 82  KPRRVFDAIIFSNELDLLDIRWRELYPYVWKFVILESHTTFTGIRKPLLFQTNRDRFAFA 141
            PRRVFDA++FSNE DLL +RW ELYPYV +FVILES++TFTG+ KPL+F++N+D+F F 
Sbjct: 107 SPRRVFDAVLFSNEKDLLTVRWNELYPYVTQFVILESNSTFTGLPKPLVFKSNKDQFKFV 166

Query: 142 ENKTVHDVFSGQVASHGSHRNPFDLESQQRVAMNGLLQRAGISNGDLLIMSDTDEIPSPH 201
           E +  +    G+        NPF  E+ QRVA++ LL+ AGI   DLLIMSD DEIPS H
Sbjct: 167 EPRLTYGTIGGRFR---KGENPFVEEAYQRVALDQLLRIAGIQEDDLLIMSDVDEIPSAH 226

Query: 202 TVELLQWCDGVPPVVHLEMRNYMYSFEFPVDYSSWRATIHIYGP-HTHYRHSRQTDLIFS 261
           T+ LL+WCD +PPV+HL+++NY+YSFE+ VD  SWRA+IH Y P  T Y H RQ++++ +
Sbjct: 227 TINLLRWCDDIPPVLHLQLKNYLYSFEYYVDSKSWRASIHRYSPGKTRYAHFRQSNVMLA 286

Query: 262 DAGWHCSFCFRNIQDFAFKMTAYSHADRVRKRDFLNYSRIQKLICQGDDLFDMLPEEYTF 321
           D+GWHCSFCFR I +F FKM AYSH+DRVR   +LN  RIQ +IC+G DLFDMLPEEYTF
Sbjct: 287 DSGWHCSFCFRYISEFIFKMKAYSHSDRVRFSHYLNPRRIQDVICKGTDLFDMLPEEYTF 346

Query: 322 QELIKKMGSIPRSSSAVHLPAYLIKNADKFKFLLPGGCVR 361
           +E+I KMG +PRS SAVHLP+YL+ NA+++K+LLPG C+R
Sbjct: 347 KEIIGKMGPVPRSYSAVHLPSYLLYNAEQYKYLLPGNCIR 383

BLAST of Lag0034306 vs. TAIR 10
Match: AT1G12990.1 (beta-1,4-N-acetylglucosaminyltransferase family protein )

HSP 1 Score: 448.0 bits (1151), Expect = 7.6e-126
Identity = 206/343 (60.06%), Postives = 264/343 (76.97%), Query Frame = 0

Query: 22  LLLLVPISVITIFNYGQKISYFFRPLWDSPPHPFVRLPHYYAENVSMEHLCRLHGWSLRS 81
           LL++VP  V+  + +GQKISYF RPLW+SPP PF  +PHYY EN SME LC+LHGW +R 
Sbjct: 53  LLVIVPTCVLAGYVHGQKISYFLRPLWESPPKPFHDIPHYYHENASMETLCKLHGWGVRD 112

Query: 82  KPRRVFDAIIFSNELDLLDIRWRELYPYVWKFVILESHTTFTGIRKPLLFQTNRDRFAFA 141
            PRRV+DA++FSNELD+L +RWREL+PY+ +FV+LES+TTFTG+ KPL+F  +RD F F 
Sbjct: 113 YPRRVYDAVLFSNELDILAVRWRELFPYITQFVLLESNTTFTGLPKPLVFAAHRDEFKFI 172

Query: 142 ENKTVHDVFSGQVASHGSHRNPFDLESQQRVAMNGLLQRAGISNGDLLIMSDTDEIPSPH 201
           E++  +    G+       +NPF  E+ QRVA++ LL+ AGI++ DLL+MSD DEIPS H
Sbjct: 173 ESRLTYGTVGGRFV---KGQNPFYEEAYQRVALDQLLRIAGITDDDLLLMSDVDEIPSRH 232

Query: 202 TVELLQWCDGVPPVVHLEMRNYMYSFEFPVDYSSWRATIHIYGP-HTHYRHSRQTDLIFS 261
           T+ LL+WCD +P ++HL ++NY+YSFEF VD  SWRA+IH Y    T Y H RQ+D I +
Sbjct: 233 TINLLRWCDEIPKILHLRLKNYLYSFEFLVDNKSWRASIHRYETGKTRYAHYRQSDEILA 292

Query: 262 DAGWHCSFCFRNIQDFAFKMTAYSHADRVRKRDFLNYSRIQKLICQGDDLFDMLPEEYTF 321
           DAGWHCSFCFR I +F FKM AYSH DRVR   FLN  R+Q++IC+G DLFDMLPEEYTF
Sbjct: 293 DAGWHCSFCFRRISEFIFKMKAYSHNDRVRFGHFLNPKRVQRVICKGADLFDMLPEEYTF 352

Query: 322 QELIKKMGSIPRSSSAVHLPAYLIKNADKFKFLLPGGCVRTPE 364
           +E+I KMG IP S SAVHLP+YL++NADK++FLLPG C+R  E
Sbjct: 353 KEIIGKMGPIPHSFSAVHLPSYLLENADKYRFLLPGNCIRESE 392

BLAST of Lag0034306 vs. TAIR 10
Match: AT1G67880.1 (beta-1,4-N-acetylglucosaminyltransferase family protein )

HSP 1 Score: 436.8 bits (1122), Expect = 1.7e-122
Identity = 201/354 (56.78%), Postives = 267/354 (75.42%), Query Frame = 0

Query: 11  RRIRPKYLLFMLLLLVPISVITIFNYGQKISYFFRPLWDSPPHPFVRLPHYYAENVSMEH 70
           R +  K +LF LL+++P+ V+ ++    KISYF RPLW+SPP PF  +PHY+ EN SME 
Sbjct: 40  RGVDLKSVLF-LLVIMPMCVLGVYINALKISYFLRPLWESPPKPFHEIPHYHHENASMES 99

Query: 71  LCRLHGWSLRSKPRRVFDAIIFSNELDLLDIRWRELYPYVWKFVILESHTTFTGIRKPLL 130
           LC+LHGW  R  PRRV+DA++FS E++LL IRW+ELYPYV +FV+LES++TFTG+ KPL+
Sbjct: 100 LCKLHGWRTREYPRRVYDAVLFSTEVELLTIRWKELYPYVTQFVLLESNSTFTGLPKPLV 159

Query: 131 FQTNRDRFAFAENKTVHDVFSGQVASHGSHRNPFDLESQQRVAMNGLLQRAGISNGDLLI 190
           F  +RD F F E +  +    G+       +NPF  E+ QR+A++ LL+ AGI++ DLLI
Sbjct: 160 FAGHRDEFKFIEPRLTYGSIGGRFKK--GEKNPFYEEAYQRIALDQLLRIAGITDDDLLI 219

Query: 191 MSDTDEIPSPHTVELLQWCDGVPPVVHLEMRNYMYSFEFPVDYSSWRATIHIYGP-HTHY 250
           MSD DEIPS HT+ LL+WCD +P ++HL ++NY+YSFEFPVD  SWRA++H Y    T Y
Sbjct: 220 MSDVDEIPSRHTINLLRWCDDIPQILHLRLKNYLYSFEFPVDDKSWRASVHRYQTGKTRY 279

Query: 251 RHSRQTDLIFSDAGWHCSFCFRNIQDFAFKMTAYSHADRVRKRDFLNYSRIQKLICQGDD 310
            H RQ+D+I +D+GWHCSFCFR I +F FKM AYSH DRVR   +LN  R+Q++IC G D
Sbjct: 280 AHYRQSDVILADSGWHCSFCFRRISEFVFKMKAYSHYDRVRFAHYLNPKRVQRVICSGSD 339

Query: 311 LFDMLPEEYTFQELIKKMGSIPRSSSAVHLPAYLIKNADKFKFLLPGGCVRTPE 364
           LFDM+PEEYTF+++I KMG IP S SAVHLPAYL++NA+++KFLLPG C+R  E
Sbjct: 340 LFDMIPEEYTFKDIIGKMGPIPHSYSAVHLPAYLLENAERYKFLLPGNCLRDEE 390

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_011656373.15.1e-20992.49beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase [Cucumis s... [more]
XP_008443970.14.3e-20892.49PREDICTED: beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase... [more]
XP_022960764.11.0e-20491.42beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like isofo... [more]
XP_023515748.12.9e-20491.42beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like isofo... [more]
KAG6589835.12.9e-20491.15Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase, partial [... [more]
Match NameE-valueIdentityDescription
Q025279.6e-1726.91Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase OS=Rattus ... [more]
Q104702.1e-1626.61Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase OS=Mus mus... [more]
Q093276.2e-1626.90Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase OS=Homo sa... [more]
Match NameE-valueIdentityDescription
A0A0A0LU592.5e-20992.49Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G351960 PE=4 SV=1[more]
A0A1S4DVT12.1e-20892.49beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase OS=Cucumis... [more]
A0A5A7U4F42.1e-20892.49Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase OS=Cucumis... [more]
A0A6J1HC234.9e-20591.42beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like isofo... [more]
A0A6J1JHS12.4e-20491.42beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like isofo... [more]
Match NameE-valueIdentityDescription
AT2G13290.19.5e-14567.95beta-1,4-N-acetylglucosaminyltransferase family protein [more]
AT2G13290.29.5e-14567.95beta-1,4-N-acetylglucosaminyltransferase family protein [more]
AT3G27540.18.9e-12759.71beta-1,4-N-acetylglucosaminyltransferase family protein [more]
AT1G12990.17.6e-12660.06beta-1,4-N-acetylglucosaminyltransferase family protein [more]
AT1G67880.11.7e-12256.78beta-1,4-N-acetylglucosaminyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006813Glycosyl transferase, family 17PFAMPF04724Glyco_transf_17coord: 16..360
e-value: 4.0E-175
score: 581.9
IPR006813Glycosyl transferase, family 17PANTHERPTHR12224BETA-1,4-MANNOSYL-GLYCOPROTEIN BETA-1,4-N-ACETYLGLUCOSAMINYL-TRANSFERASEcoord: 9..363
NoneNo IPR availablePANTHERPTHR12224:SF23GLYCOSYL TRANSFERASE, FAMILY 17-RELATEDcoord: 9..363

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0034306.1Lag0034306.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009734 auxin-activated signaling pathway
biological_process GO:0006044 N-acetylglucosamine metabolic process
biological_process GO:0006487 protein N-linked glycosylation
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
cellular_component GO:0016020 membrane
molecular_function GO:0003830 beta-1,4-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity