Lag0034274 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0034274 vs. NCBI nr
Match: XP_038880237.1 (uncharacterized vacuolar membrane protein YML018C-like [Benincasa hispida])

HSP 1 Score: 686.0 bits (1769), Expect = 2.0e-193
Identity = 376/435 (86.44%), Postives = 397/435 (91.26%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYK GLILL+AVVVIWV+SAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI
Sbjct: 1   MAWKYKVGLILLVAVVVIWVASAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60

Query: 61  MKLFRSHFRNGNSKKVAEIQP--PAIEERTSQSSSIEEDNNNNNNNNNNNNNISEVENGD 120
           +K+FRSHFRNGNS+KVAE+QP  PA+EERT+Q SSIE+D  +NNNNNNNNN ISEV NGD
Sbjct: 61  VKIFRSHFRNGNSRKVAELQPPLPALEERTNQ-SSIEDD--DNNNNNNNNNIISEVGNGD 120

Query: 121 VDNCVVNIECEDQEEEEVRVNK---NGGCECEGEGEKGGMRLLKTEEITKFSTKQIAVLA 180
           V  CVVNI     + E++RVN    NGGCECE E  +  M +LKTEE TK STKQIAVLA
Sbjct: 121 V-QCVVNI----IQGEDLRVNNNNHNGGCECEPENLE--MTILKTEE-TKCSTKQIAVLA 180

Query: 181 LAIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVS 240
           L IGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDAC+ERQSLTIVNVVAV VS
Sbjct: 181 LVIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDACLERQSLTIVNVVAVIVS 240

Query: 241 MAGVAMTTVGKTWARDEAHSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEK 300
           MAGVAMTTVGKTWARDE  SSSS HGKHSYVGD+FALLSA TDGLYYVLLKKYAGEEGEK
Sbjct: 241 MAGVAMTTVGKTWARDEVRSSSSEHGKHSYVGDIFALLSALTDGLYYVLLKKYAGEEGEK 300

Query: 301 VDMQKFLGYVGLFTLTTLWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYF 360
           VDMQKFLGYVGLFTLTTLWWL+WPLRAIGIEPKF IPQSTKVAE+VLANCFV+NFVSDYF
Sbjct: 301 VDMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFMIPQSTKVAELVLANCFVSNFVSDYF 360

Query: 361 WAMGVVWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANLSDWI 420
           WAMGVVWTSPLVAALGASLTIPLAMLGDMVLHGRHYSL+YI GSVQVFLGFMIANLSDWI
Sbjct: 361 WAMGVVWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLIYIFGSVQVFLGFMIANLSDWI 420

Query: 421 SPKLKLRKMFFNGTK 431
           SPKLKLRK FFNG K
Sbjct: 421 SPKLKLRKKFFNGAK 424

BLAST of Lag0034274 vs. NCBI nr
Match: XP_022925420.1 (uncharacterized transporter C405.03c-like [Cucurbita moschata])

HSP 1 Score: 664.5 bits (1713), Expect = 6.4e-187
Identity = 357/430 (83.02%), Postives = 382/430 (88.84%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYKGGLILL+AVVVIWV+SAEITQSIFTDYEHPFV+TYVGTSMLVAYLAIAF++ECI
Sbjct: 1   MAWKYKGGLILLVAVVVIWVASAEITQSIFTDYEHPFVMTYVGTSMLVAYLAIAFVRECI 60

Query: 61  MKLFRSHFRNGNSKKVAEIQPPAIEERTSQSSSIEEDNNNNNNNNNNNNNISEVENGDVD 120
           +KLFRSHF NGN K+VAEIQ              E+DNNNNNNNNNNNNNISEVENGDV 
Sbjct: 61  VKLFRSHFPNGNPKEVAEIQ--------------EQDNNNNNNNNNNNNNISEVENGDV- 120

Query: 121 NCVVNIECEDQEEEEVRVNKNGGCECEGEGEKGGMRLLKTEEITKFSTKQIAVLALAIGP 180
           +CVVN+ECE+QE+     + NG CECE E          T+E T FSTKQ+AVLALAIGP
Sbjct: 121 HCVVNVECEEQEKGS--ASNNGRCECEAEN--------GTQE-TSFSTKQMAVLALAIGP 180

Query: 181 IWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAGVA 240
           IWFVSEYFTNAALA+TSVATTTILFSTSGLFTLI+DAC ERQSL+IVNVVAVFVSMAGVA
Sbjct: 181 IWFVSEYFTNAALAKTSVATTTILFSTSGLFTLILDACFERQSLSIVNVVAVFVSMAGVA 240

Query: 241 MTTVGKTWARDEAHSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQK 300
           MTTVGKTWARDEA  SS GH KHSY+GDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQK
Sbjct: 241 MTTVGKTWARDEA-QSSFGHEKHSYIGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQK 300

Query: 301 FLGYVGLFTLTTLWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWAMGV 360
           FLGYVGLFTLTTLWWL+WPLRAIGIEPKF +PQSTKVAEIVLANCFV+NFVSDY WAMGV
Sbjct: 301 FLGYVGLFTLTTLWWLIWPLRAIGIEPKFGLPQSTKVAEIVLANCFVSNFVSDYLWAMGV 360

Query: 361 VWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANLSDWISPKLK 420
           VWTSPLVAALGASLTIPLAM+GDM+LHGRHYSLVYI GS+QVFLGFMIAN SDWISPKLK
Sbjct: 361 VWTSPLVAALGASLTIPLAMVGDMLLHGRHYSLVYIFGSLQVFLGFMIANFSDWISPKLK 403

Query: 421 LRKMFFNGTK 431
           LRK FFNG K
Sbjct: 421 LRKKFFNGAK 403

BLAST of Lag0034274 vs. NCBI nr
Match: XP_023531720.1 (uncharacterized transporter C405.03c-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 660.6 bits (1703), Expect = 9.2e-186
Identity = 358/430 (83.26%), Postives = 382/430 (88.84%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYKGGLILL+AVVVIWV+SAEITQSIFTDYEHPFV+TYVGTSMLVAYLAIAFI+ECI
Sbjct: 1   MAWKYKGGLILLVAVVVIWVASAEITQSIFTDYEHPFVMTYVGTSMLVAYLAIAFIRECI 60

Query: 61  MKLFRSHFRNGNSKKVAEIQPPAIEERTSQSSSIEEDNNNNNNNNNNNNNISEVENGDVD 120
           +KLFRSHF N NSK+VAEIQ         +  +   +NN NNNNNNNNNNISEVENGDV 
Sbjct: 61  VKLFRSHFPNRNSKEVAEIQ---------EQDNNNNNNNTNNNNNNNNNNISEVENGDV- 120

Query: 121 NCVVNIECEDQEEEEVRVNKNGGCECEGEGEKGGMRLLKTEEITKFSTKQIAVLALAIGP 180
           +CVVNIECE+QE+    V+ NG CECE E          T+E T FSTKQ+AVLALAIGP
Sbjct: 121 HCVVNIECEEQEKGS--VSNNGRCECEAEN--------GTQE-TPFSTKQMAVLALAIGP 180

Query: 181 IWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAGVA 240
           IWFVSEYFTNAALARTSVATTTILFSTSGLFTLI+DAC ERQSL+IVNVVAVFVSMAGVA
Sbjct: 181 IWFVSEYFTNAALARTSVATTTILFSTSGLFTLILDACFERQSLSIVNVVAVFVSMAGVA 240

Query: 241 MTTVGKTWARDEAHSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQK 300
           MTTVGKTWARDE   SS GH KHSY+GDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQK
Sbjct: 241 MTTVGKTWARDET-QSSFGHEKHSYIGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQK 300

Query: 301 FLGYVGLFTLTTLWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWAMGV 360
           FLGYVGLFTLTTLWWL+WPLRA+GIEPKF IPQSTKVAEIVLANCFV+NFVSDYFWAMGV
Sbjct: 301 FLGYVGLFTLTTLWWLIWPLRAMGIEPKFGIPQSTKVAEIVLANCFVSNFVSDYFWAMGV 360

Query: 361 VWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANLSDWISPKLK 420
           VWTSPLVAALGASLTIPLAM+GDM+LHGRHYSLVYI GS+QVFLGFMIAN SDWISPKLK
Sbjct: 361 VWTSPLVAALGASLTIPLAMVGDMLLHGRHYSLVYIFGSLQVFLGFMIANFSDWISPKLK 408

Query: 421 LRKMFFNGTK 431
           LRK FFNG K
Sbjct: 421 LRKKFFNGAK 408

BLAST of Lag0034274 vs. NCBI nr
Match: XP_023007421.1 (uncharacterized vacuolar membrane protein YML018C-like [Cucurbita maxima])

HSP 1 Score: 651.4 bits (1679), Expect = 5.6e-183
Identity = 352/430 (81.86%), Postives = 379/430 (88.14%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYKGGLILL+ VVVIWV+SAEITQSIF DYEHPFV+TYVGTSMLVAYLAIAF++EC+
Sbjct: 1   MAWKYKGGLILLVVVVVIWVASAEITQSIFMDYEHPFVMTYVGTSMLVAYLAIAFLRECL 60

Query: 61  MKLFRSHFRNGNSKKVAEIQPPAIEERTSQSSSIEEDNNNNNNNNNNNNNISEVENGDVD 120
           +KLFRSHFRNGNSK+VAEIQ         +      +NN NNNNNNNNNNISEVENGDV 
Sbjct: 61  VKLFRSHFRNGNSKEVAEIQ---------EQDKDNNNNNINNNNNNNNNNISEVENGDV- 120

Query: 121 NCVVNIECEDQEEEEVRVNKNGGCECEGEGEKGGMRLLKTEEITKFSTKQIAVLALAIGP 180
            CVVNIECE+QE+    V+ N  CECE E          T+E T FSTK+IAVLALAIGP
Sbjct: 121 LCVVNIECEEQEKGS--VSNNDRCECEAEN--------GTQE-TSFSTKKIAVLALAIGP 180

Query: 181 IWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAGVA 240
           IWFVSEYFTNAALARTSVATTTILFSTSGLFTLI+DAC ERQSL+IVNVVAVFVSMAGVA
Sbjct: 181 IWFVSEYFTNAALARTSVATTTILFSTSGLFTLILDACFERQSLSIVNVVAVFVSMAGVA 240

Query: 241 MTTVGKTWARDEAHSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQK 300
           MTT+GKTWARDEA SSS  H KHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQK
Sbjct: 241 MTTIGKTWARDEAQSSSR-HEKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQK 300

Query: 301 FLGYVGLFTLTTLWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWAMGV 360
           FLGYVGLFTLTTLWWL+WPL+AIGIEPKF IPQSTKVAEIV+ANCFV+NFVSDY WAMGV
Sbjct: 301 FLGYVGLFTLTTLWWLIWPLKAIGIEPKFGIPQSTKVAEIVIANCFVSNFVSDYLWAMGV 360

Query: 361 VWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANLSDWISPKLK 420
           VWTSPL+AALGASLTIPLAM+GDM+LHGRHYSLVYI GS+QVFLGFMIAN SDWISPKLK
Sbjct: 361 VWTSPLIAALGASLTIPLAMVGDMLLHGRHYSLVYIFGSLQVFLGFMIANFSDWISPKLK 408

Query: 421 LRKMFFNGTK 431
           LRK FF+G K
Sbjct: 421 LRKKFFDGAK 408

BLAST of Lag0034274 vs. NCBI nr
Match: TYK06345.1 (putative transporter-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 643.3 bits (1658), Expect = 1.5e-180
Identity = 354/433 (81.76%), Postives = 373/433 (86.14%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYK GLILL+AVVVIWV+SAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI
Sbjct: 1   MAWKYKVGLILLVAVVVIWVTSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60

Query: 61  MKLFRSHFR-NGNSKKVAEIQP--PAIEERTSQSSSIEEDNNNNNNNNNNNNNISEVENG 120
           MK+ RSHFR  GN +KVAEIQ   PA+EERT+QSS           NNNNN    +VEN 
Sbjct: 61  MKIVRSHFRTKGNCRKVAEIQSPLPALEERTNQSS-----------NNNNNTTSDKVENR 120

Query: 121 DVDNCVVNIECEDQEEEEVRVNKNGGCECEGEGEKGGMRLLKTEEITKFSTKQIAVLALA 180
           DV   VVNI  ++        N NGGCE   E E   MR+LKTEE  KFSTKQIAVLAL 
Sbjct: 121 DV-QYVVNIN-DNNNNSNNSSNNNGGCELR-EAENMEMRILKTEE-AKFSTKQIAVLALT 180

Query: 181 IGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMA 240
           IGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLI+DAC+ERQSLTIVNVVAV VSMA
Sbjct: 181 IGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLILDACLERQSLTIVNVVAVVVSMA 240

Query: 241 GVAMTTVGKTWARDEAHSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVD 300
           GVAMTTVGKTWA+DE  SSS GHGKHS+VGD FALLSA TDGLYYVLLKKYAGEEGEKVD
Sbjct: 241 GVAMTTVGKTWAKDEPQSSSYGHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKVD 300

Query: 301 MQKFLGYVGLFTLTTLWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWA 360
           MQKFLGYVGLFTLTTLWWL+WPLRAIGIEPKF IPQSTKVAE+VLANCFVANFVSDYFWA
Sbjct: 301 MQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFMIPQSTKVAEVVLANCFVANFVSDYFWA 360

Query: 361 MGVVWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANLSDWISP 420
           MGVVWTSPLVAALGASLTIPLAM+GDMVLHGRHYSLVYI GS+QVFLGF+IAN SDWISP
Sbjct: 361 MGVVWTSPLVAALGASLTIPLAMIGDMVLHGRHYSLVYIFGSIQVFLGFIIANFSDWISP 418

Query: 421 KLKLRKMFFNGTK 431
           KLKLRK FFN  K
Sbjct: 421 KLKLRKKFFNEAK 418

BLAST of Lag0034274 vs. ExPASy Swiss-Prot
Match: Q03730 (Uncharacterized vacuolar membrane protein YML018C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YML018C PE=1 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 6.5e-33
Identity = 124/411 (30.17%), Postives = 190/411 (46.23%), Query Frame = 0

Query: 8   GLILLIAVVVIWVSSAEITQSIFTD--YEHPFVVTYVGTSMLVAYLAIAFIKECIMKLFR 67
           GL++L  V+++WV S+ +   IF D  Y  PF +TY  T+  + YL     K  ++    
Sbjct: 16  GLLMLGLVIILWVLSSFLINLIFEDDSYRKPFFITYTNTAAFIFYL-FPTAKAVVVNYKD 75

Query: 68  SHFRNGNSKKVAEIQPPAIEERTSQSSSIEEDNNNNNNNNNNNNNISEVENGDVDNCVVN 127
           +   N + + + E      EE T   S+   D  +                      + N
Sbjct: 76  TGRANVHRELIME------EEGTGSDSNRSVDMTS--------------------PLLTN 135

Query: 128 IECEDQEEEEVRVNKNGGCECEGEGEKGGMRLLKTEEITKFSTKQIAVLALAIGPIWFVS 187
           +E                          G    + + +T + T +   L+     +WF +
Sbjct: 136 LE-------------------------AGTHANQKKRLTLYETIK---LSAEFCILWFTA 195

Query: 188 EYFTNAALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAGVAMTTVG 247
              TNA+LA TSVA+ TIL +TS  FTL I A    +SL+   V+  F+S  G+ M T  
Sbjct: 196 NLVTNASLAFTSVASQTILSTTSSFFTLFIGAICHVESLSKSKVLGSFISFVGIIMVTKS 255

Query: 248 KTWARDEAH----SSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKF 307
            +  R + H    S          +G++ AL  A   G+Y  LLK+  G+E  +V+M+ F
Sbjct: 256 DSHQRYQRHIADVSGDDNDAVQVLIGNLLALAGAVLYGVYSTLLKREVGDE-TRVNMKIF 315

Query: 308 LGYVGLFTLTTLWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWAMGVV 367
            G+VGLF L  LW  +  L   G EP F++P+  KV  I+  NC +  FVSD+ WA  ++
Sbjct: 316 FGFVGLFNLLFLWPSLIVLDFFGWEP-FSLPKDPKVVVIIFVNCLI-TFVSDFCWAKAML 368

Query: 368 WTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANLS 413
            TSPL   +G S+TIPLAM GD++   +  S +Y+ G+  +   F I N S
Sbjct: 376 LTSPLTVTVGLSITIPLAMFGDVIFKHKTMSALYLFGATLILGSFFIINKS 368

BLAST of Lag0034274 vs. ExPASy Swiss-Prot
Match: O94654 (Uncharacterized transporter C405.03c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC405.03c PE=3 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 2.3e-30
Identity = 89/253 (35.18%), Postives = 140/253 (55.34%), Query Frame = 0

Query: 158 LKTEEITKFSTKQIAVLALAIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDA 217
           L T   +    +Q A L+L    IWF + YF+N++L  T+VA+ TI+ S SG FTL +  
Sbjct: 89  LGTRPNSPLGFRQTAYLSLGFCIIWFAANYFSNSSLGFTNVASFTIISSMSGFFTLGLGT 148

Query: 218 CMERQSLTIVNVVAVFVSMAGVAMTTVGKTWARDEAHSSSSGHGKHSYVGDVFALLSAFT 277
            +  +  T+  ++A+  S+ GV +         D   S  S       +G+ +ALL+A  
Sbjct: 149 IVNVERFTLSKLLALMASVGGVIIVVTQDAKQADLNDSPPS----RPALGNAYALLAALL 208

Query: 278 DGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTLTTLWWLVWPLRAIGIEPKFAIPQSTKV 337
            G Y V++K +  EE   V  + F G VGLF L  LW  +  L   G+E +F++P +T  
Sbjct: 209 YGCYSVMVKFHITEE-SCVSTRLFFGLVGLFDLILLWPFLIILHLYGVE-RFSLPSTTAG 268

Query: 338 AEIVLANCFVANFVSDYFWAMGVVWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYIL 397
             +++ N  +  FVSDY W + ++ TSPL+  +G SL+IPLA+  D++L G + +   IL
Sbjct: 269 LIVLIINASI-TFVSDYLWVIAMLMTSPLLVTVGMSLSIPLALFFDILLKGHYLNFSLIL 328

Query: 398 GSVQVFLGFMIAN 411
           GS+ VF GF++ N
Sbjct: 329 GSLLVFAGFIVVN 334

BLAST of Lag0034274 vs. ExPASy Swiss-Prot
Match: A6QL92 (Solute carrier family 35 member F5 OS=Bos taurus OX=9913 GN=SLC35F5 PE=2 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 4.1e-27
Identity = 127/442 (28.73%), Postives = 212/442 (47.96%), Query Frame = 0

Query: 8   GLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECIMKLFRSH 67
           G+++L+ V VIWV+S+E+T  +FT Y  PF  T+  TSM V YL + FI   + K +R  
Sbjct: 72  GIVILLLVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVLYL-LGFI---VWKPWRQQ 131

Query: 68  ----FRNGNSKKVAEIQP--PAIEERTSQSSSIEE-----------DNNNNNNNNNNNNN 127
               FR  ++   A+ +    A    T+ +SS+ E            +    N N +   
Sbjct: 132 CTRGFRGKHATFFADAEGYFAACTTDTTMNSSLSEPLYVPVKFHDLPSEKPENTNIDTEK 191

Query: 128 ISEVENGDVDNCVVNIECEDQEEEEVRVNKNGGCECEGEGEKGGMRLLKTEEITKFSTKQ 187
           I +       N +   +       E ++++           K    LLKT  + K +  Q
Sbjct: 192 IPKKSRVRFSNIMEIRQLPSSHALEAKLSR-----MSYPTVKEQESLLKT--VGKLTATQ 251

Query: 188 IAVLALAIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDACMERQS---LTIV 247
           +A ++     +WF++ +    AL+ T VA   IL STSGLFTLI+ A     S    T+ 
Sbjct: 252 VAKISFFFCFVWFLANFSYQEALSDTQVAIVNILSSTSGLFTLILAAMFPSNSGDRFTLS 311

Query: 248 NVVAVFVSMAGVAMTTVGKTWARDEAHSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKK 307
            ++AV +S+ GV +  +           S    G+++ +G +++L+ A    +Y V++K+
Sbjct: 312 KLLAVILSIGGVVLVNLS---------GSEKSPGRNT-IGSIWSLVGAMLYAVYIVMIKR 371

Query: 308 YAGEEGEKVDMQKFLGYVGLFTLTTLWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFV 367
               E +K+D+  F G+VGLF L  LW   + L   G E  F  P    +  IV+ N  +
Sbjct: 372 KVDRE-DKLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFE-DFEFPNKVVLMCIVI-NGLI 431

Query: 368 ANFVSDYFWAMGVVWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFM 426
              +S++ W  G   TS L+  L  SLTIPL+++ DM +    +S ++  G++ VF  F 
Sbjct: 432 GTVLSEFLWLWGCFLTSSLIGTLALSLTIPLSIIADMCMQKVQFSWLFFAGAIPVFFSFF 489

BLAST of Lag0034274 vs. ExPASy Swiss-Prot
Match: Q5R6J3 (Solute carrier family 35 member F5 OS=Pongo abelii OX=9601 GN=SLC35F5 PE=2 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 2.9e-25
Identity = 128/436 (29.36%), Postives = 202/436 (46.33%), Query Frame = 0

Query: 8   GLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECIMKLFRSH 67
           G+++L+ V VIWV+S+E+T  +FT Y  PF  T+  TSM V YL + FI   I K +R  
Sbjct: 72  GIVILLLVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVLYL-LGFI---IWKPWRQQ 131

Query: 68  FRNGNSKK------VAEIQPPAIEERTSQSSSIEEDNNNNNNNNNNNNNISEVENGDVDN 127
              G   K       AE    A    T+ +SS+ E        ++  +   E  N D + 
Sbjct: 132 CTRGLRGKHAAFFADAEGYFAACATDTTMNSSLSEPLYVPVKFHDLPSEKPESTNIDTEK 191

Query: 128 CVVNIECEDQEEEEVR-VNKNGGCECE----GEGEKGGMRLLKTEEITKFSTKQIAVLAL 187
                        E+R +  N   E +        K    +LKT  + K +  Q+A ++ 
Sbjct: 192 TPKKSRVRFSNIMEIRQLPSNHALESKLSRMSYPVKEQESILKT--VGKLTATQVAKISF 251

Query: 188 AIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDACMERQS---LTIVNVVAVF 247
               +WF++      AL+ T VA   IL STSGLFTLI+ A     S    T+  ++AV 
Sbjct: 252 FFCFVWFLANLSYQEALSDTQVAIVNILSSTSGLFTLILAAVFPSNSGDRFTLSKLLAVI 311

Query: 248 VSMAGVAMTTVGKTWARDEAHSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEG 307
           +S+ GV +  +          S S        +G +++L  A    +Y V++K+    E 
Sbjct: 312 LSIGGVVLVNL----------SGSEKSAGRDTIGSIWSLAGAMLYAVYIVMIKRKVDRE- 371

Query: 308 EKVDMQKFLGYVGLFTLTTLWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSD 367
           +K+D+  F G+VGLF L  LW   + L   G E  F  P    V   ++ N  +   +S+
Sbjct: 372 DKLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFE-DFEFPNKV-VLMCIIINGLIGTVLSE 431

Query: 368 YFWAMGVVWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANL-- 426
           + W  G   TS L+  L  SLTIPL+++ DM +    +S ++  G++ VF  F I  L  
Sbjct: 432 FLWLWGCFLTSSLIGTLALSLTIPLSIIADMCMQKVQFSWLFFAGAIPVFFSFFIVTLLC 488

BLAST of Lag0034274 vs. ExPASy Swiss-Prot
Match: Q04083 (Thiamine-repressible mitochondrial transport protein THI74 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=THI74 PE=1 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 5.0e-25
Identity = 109/411 (26.52%), Postives = 188/411 (45.74%), Query Frame = 0

Query: 8   GLILLIAVVVIWVSSAEITQSIF--TDYEHPFVVTYVGTSMLVAYLAIAFIKECIMKLFR 67
           G++LL  VVV WV ++ +T  +     Y  PF +TY+  S    YL              
Sbjct: 13  GVLLLAVVVVFWVGASCLTNELLETNAYNKPFFLTYLNISSFALYL-------------- 72

Query: 68  SHFRNGNSKKVAEIQPPAIEERTSQSSSIEEDNNNN------NNNNNNNNNISEVENGDV 127
                 +  ++ + +  +++ERT ++  I    + +      ++  + ++N+S + +  V
Sbjct: 73  ----TPDLWRIIQSRRKSLQERTERTLPIHTQESFSEFLPLLSSTPSTSSNLSSIADTKV 132

Query: 128 DNCVVNIECEDQEEEEVRVNKNGGCECEGEGEKGGMRLLKTEEITKFSTKQIAVLALAIG 187
                                           K  MR                 L+L   
Sbjct: 133 --------------------------------KDTMR-----------------LSLLFC 192

Query: 188 PIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAGV 247
            +WFV+    NAAL+ T+VA++TIL STS  FTL +   +  ++ +   ++ +FVS+ G+
Sbjct: 193 VLWFVANLAANAALSYTTVASSTILSSTSSFFTLFLATSLGIETFSTKKLLGLFVSLFGI 252

Query: 248 AMTTVGKTWARDEAHSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQ 307
            +  +  +  +D   +SS        VG+  ALL +    +Y  LLK     +G ++D+Q
Sbjct: 253 ILIVMQSSKQQDSVSASS------FLVGNTLALLGSLGYSVYTTLLKYEISSKGLRLDIQ 312

Query: 308 KFLGYVGLFTLTTLWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWAMG 367
            FLGYVG+FT    W ++  L    +E  F +P +  ++ +V+ NC +  FVSDYFW   
Sbjct: 313 MFLGYVGIFTFLLFWPILIILDITHME-TFELPSNFHISFLVMLNCIII-FVSDYFWCKA 348

Query: 368 VVWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIAN 411
           ++ TSPLV  +  + TIPLAM  D V     ++  YI+G + +F+ F + N
Sbjct: 373 LILTSPLVVTVALTFTIPLAMFADFVWREAFFTPWYIIGVIFIFVSFFLVN 348

BLAST of Lag0034274 vs. ExPASy TrEMBL
Match: A0A6J1EHX2 (uncharacterized transporter C405.03c-like OS=Cucurbita moschata OX=3662 GN=LOC111432715 PE=4 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 3.1e-187
Identity = 357/430 (83.02%), Postives = 382/430 (88.84%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYKGGLILL+AVVVIWV+SAEITQSIFTDYEHPFV+TYVGTSMLVAYLAIAF++ECI
Sbjct: 1   MAWKYKGGLILLVAVVVIWVASAEITQSIFTDYEHPFVMTYVGTSMLVAYLAIAFVRECI 60

Query: 61  MKLFRSHFRNGNSKKVAEIQPPAIEERTSQSSSIEEDNNNNNNNNNNNNNISEVENGDVD 120
           +KLFRSHF NGN K+VAEIQ              E+DNNNNNNNNNNNNNISEVENGDV 
Sbjct: 61  VKLFRSHFPNGNPKEVAEIQ--------------EQDNNNNNNNNNNNNNISEVENGDV- 120

Query: 121 NCVVNIECEDQEEEEVRVNKNGGCECEGEGEKGGMRLLKTEEITKFSTKQIAVLALAIGP 180
           +CVVN+ECE+QE+     + NG CECE E          T+E T FSTKQ+AVLALAIGP
Sbjct: 121 HCVVNVECEEQEKGS--ASNNGRCECEAEN--------GTQE-TSFSTKQMAVLALAIGP 180

Query: 181 IWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAGVA 240
           IWFVSEYFTNAALA+TSVATTTILFSTSGLFTLI+DAC ERQSL+IVNVVAVFVSMAGVA
Sbjct: 181 IWFVSEYFTNAALAKTSVATTTILFSTSGLFTLILDACFERQSLSIVNVVAVFVSMAGVA 240

Query: 241 MTTVGKTWARDEAHSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQK 300
           MTTVGKTWARDEA  SS GH KHSY+GDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQK
Sbjct: 241 MTTVGKTWARDEA-QSSFGHEKHSYIGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQK 300

Query: 301 FLGYVGLFTLTTLWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWAMGV 360
           FLGYVGLFTLTTLWWL+WPLRAIGIEPKF +PQSTKVAEIVLANCFV+NFVSDY WAMGV
Sbjct: 301 FLGYVGLFTLTTLWWLIWPLRAIGIEPKFGLPQSTKVAEIVLANCFVSNFVSDYLWAMGV 360

Query: 361 VWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANLSDWISPKLK 420
           VWTSPLVAALGASLTIPLAM+GDM+LHGRHYSLVYI GS+QVFLGFMIAN SDWISPKLK
Sbjct: 361 VWTSPLVAALGASLTIPLAMVGDMLLHGRHYSLVYIFGSLQVFLGFMIANFSDWISPKLK 403

Query: 421 LRKMFFNGTK 431
           LRK FFNG K
Sbjct: 421 LRKKFFNGAK 403

BLAST of Lag0034274 vs. ExPASy TrEMBL
Match: A0A6J1L0H0 (uncharacterized vacuolar membrane protein YML018C-like OS=Cucurbita maxima OX=3661 GN=LOC111499921 PE=4 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 2.7e-183
Identity = 352/430 (81.86%), Postives = 379/430 (88.14%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYKGGLILL+ VVVIWV+SAEITQSIF DYEHPFV+TYVGTSMLVAYLAIAF++EC+
Sbjct: 1   MAWKYKGGLILLVVVVVIWVASAEITQSIFMDYEHPFVMTYVGTSMLVAYLAIAFLRECL 60

Query: 61  MKLFRSHFRNGNSKKVAEIQPPAIEERTSQSSSIEEDNNNNNNNNNNNNNISEVENGDVD 120
           +KLFRSHFRNGNSK+VAEIQ         +      +NN NNNNNNNNNNISEVENGDV 
Sbjct: 61  VKLFRSHFRNGNSKEVAEIQ---------EQDKDNNNNNINNNNNNNNNNISEVENGDV- 120

Query: 121 NCVVNIECEDQEEEEVRVNKNGGCECEGEGEKGGMRLLKTEEITKFSTKQIAVLALAIGP 180
            CVVNIECE+QE+    V+ N  CECE E          T+E T FSTK+IAVLALAIGP
Sbjct: 121 LCVVNIECEEQEKGS--VSNNDRCECEAEN--------GTQE-TSFSTKKIAVLALAIGP 180

Query: 181 IWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAGVA 240
           IWFVSEYFTNAALARTSVATTTILFSTSGLFTLI+DAC ERQSL+IVNVVAVFVSMAGVA
Sbjct: 181 IWFVSEYFTNAALARTSVATTTILFSTSGLFTLILDACFERQSLSIVNVVAVFVSMAGVA 240

Query: 241 MTTVGKTWARDEAHSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQK 300
           MTT+GKTWARDEA SSS  H KHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQK
Sbjct: 241 MTTIGKTWARDEAQSSSR-HEKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQK 300

Query: 301 FLGYVGLFTLTTLWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWAMGV 360
           FLGYVGLFTLTTLWWL+WPL+AIGIEPKF IPQSTKVAEIV+ANCFV+NFVSDY WAMGV
Sbjct: 301 FLGYVGLFTLTTLWWLIWPLKAIGIEPKFGIPQSTKVAEIVIANCFVSNFVSDYLWAMGV 360

Query: 361 VWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANLSDWISPKLK 420
           VWTSPL+AALGASLTIPLAM+GDM+LHGRHYSLVYI GS+QVFLGFMIAN SDWISPKLK
Sbjct: 361 VWTSPLIAALGASLTIPLAMVGDMLLHGRHYSLVYIFGSLQVFLGFMIANFSDWISPKLK 408

Query: 421 LRKMFFNGTK 431
           LRK FF+G K
Sbjct: 421 LRKKFFDGAK 408

BLAST of Lag0034274 vs. ExPASy TrEMBL
Match: A0A5D3C4N7 (Putative transporter-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G00180 PE=4 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 7.4e-181
Identity = 354/433 (81.76%), Postives = 373/433 (86.14%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYK GLILL+AVVVIWV+SAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI
Sbjct: 1   MAWKYKVGLILLVAVVVIWVTSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60

Query: 61  MKLFRSHFR-NGNSKKVAEIQP--PAIEERTSQSSSIEEDNNNNNNNNNNNNNISEVENG 120
           MK+ RSHFR  GN +KVAEIQ   PA+EERT+QSS           NNNNN    +VEN 
Sbjct: 61  MKIVRSHFRTKGNCRKVAEIQSPLPALEERTNQSS-----------NNNNNTTSDKVENR 120

Query: 121 DVDNCVVNIECEDQEEEEVRVNKNGGCECEGEGEKGGMRLLKTEEITKFSTKQIAVLALA 180
           DV   VVNI  ++        N NGGCE   E E   MR+LKTEE  KFSTKQIAVLAL 
Sbjct: 121 DV-QYVVNIN-DNNNNSNNSSNNNGGCELR-EAENMEMRILKTEE-AKFSTKQIAVLALT 180

Query: 181 IGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMA 240
           IGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLI+DAC+ERQSLTIVNVVAV VSMA
Sbjct: 181 IGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLILDACLERQSLTIVNVVAVVVSMA 240

Query: 241 GVAMTTVGKTWARDEAHSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVD 300
           GVAMTTVGKTWA+DE  SSS GHGKHS+VGD FALLSA TDGLYYVLLKKYAGEEGEKVD
Sbjct: 241 GVAMTTVGKTWAKDEPQSSSYGHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKVD 300

Query: 301 MQKFLGYVGLFTLTTLWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWA 360
           MQKFLGYVGLFTLTTLWWL+WPLRAIGIEPKF IPQSTKVAE+VLANCFVANFVSDYFWA
Sbjct: 301 MQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFMIPQSTKVAEVVLANCFVANFVSDYFWA 360

Query: 361 MGVVWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANLSDWISP 420
           MGVVWTSPLVAALGASLTIPLAM+GDMVLHGRHYSLVYI GS+QVFLGF+IAN SDWISP
Sbjct: 361 MGVVWTSPLVAALGASLTIPLAMIGDMVLHGRHYSLVYIFGSIQVFLGFIIANFSDWISP 418

Query: 421 KLKLRKMFFNGTK 431
           KLKLRK FFN  K
Sbjct: 421 KLKLRKKFFNEAK 418

BLAST of Lag0034274 vs. ExPASy TrEMBL
Match: A0A5A7U493 (Putative transporter-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00790 PE=4 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 1.1e-179
Identity = 353/434 (81.34%), Postives = 372/434 (85.71%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYK GLILL+AVVVIWV+SAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI
Sbjct: 1   MAWKYKVGLILLVAVVVIWVTSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60

Query: 61  MKLFRSHFR-NGNSKKVAEIQP--PAIEERTSQSSSIEEDNNNNNNNNNNNNNISEVENG 120
           MK+ RSHFR  GN +KVAEIQ   PA+EERT+QSS           NNNNN    EVEN 
Sbjct: 61  MKIVRSHFRTKGNCRKVAEIQSPLPALEERTNQSS-----------NNNNNTTSDEVENR 120

Query: 121 DVDNCVVNIECEDQEEEEVRVNKNGGCE-CEGEGEKGGMRLLKTEEITKFSTKQIAVLAL 180
           DV   VVNI  ++        N NGGCE CE E  +  MR+LKTEE  KFSTKQIAVLAL
Sbjct: 121 DV-QYVVNIN-DNNNNSNNSSNNNGGCELCEAENME--MRILKTEE-AKFSTKQIAVLAL 180

Query: 181 AIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSM 240
            IGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLI+DAC+ERQSLTIVN VAV VSM
Sbjct: 181 TIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLILDACLERQSLTIVNAVAVVVSM 240

Query: 241 AGVAMTTVGKTWARDEAHSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKV 300
           AGV MTTVGKTWA+DE  SSS GHGKHS+VGD FALLSA TDGLYYVLLKKYAGEEGEKV
Sbjct: 241 AGVVMTTVGKTWAKDEPQSSSYGHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKV 300

Query: 301 DMQKFLGYVGLFTLTTLWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFW 360
           DMQKFLGYVGLFTLTTLWWL+WPLRAIGIEPKF IPQSTKVAE+VLANCFVANFVSDYFW
Sbjct: 301 DMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFMIPQSTKVAEVVLANCFVANFVSDYFW 360

Query: 361 AMGVVWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANLSDWIS 420
           AMGVVWTSPLVAALGASLTIPLAM+GDMVLH RHYSLVYI GS+QVFLGF+IAN SDWIS
Sbjct: 361 AMGVVWTSPLVAALGASLTIPLAMIGDMVLHDRHYSLVYIFGSIQVFLGFIIANFSDWIS 418

Query: 421 PKLKLRKMFFNGTK 431
           PKLKLRK FFN  K
Sbjct: 421 PKLKLRKKFFNEAK 418

BLAST of Lag0034274 vs. ExPASy TrEMBL
Match: A0A6J1C0M9 (uncharacterized transporter C405.03c-like OS=Momordica charantia OX=3673 GN=LOC111006993 PE=4 SV=1)

HSP 1 Score: 630.2 bits (1624), Expect = 6.4e-177
Identity = 342/429 (79.72%), Postives = 374/429 (87.18%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAW+YKGGLILLIAVVVIWVSSAEITQSIF DYEHPFV+TY+GTSMLVAYLAIAFIKECI
Sbjct: 1   MAWRYKGGLILLIAVVVIWVSSAEITQSIFRDYEHPFVMTYIGTSMLVAYLAIAFIKECI 60

Query: 61  MKLFRSHFRNGNSKKVAEIQPPAIEERTSQSSSIEEDNNNNNNNNNNNNNISEVENGDVD 120
           +KLFR+HF NGNSKKVAE+  P + E  S       +N ++ NNNNNNNNISEV +G   
Sbjct: 61  VKLFRTHFSNGNSKKVAEVAQPQVMEEKS-------NNPSSPNNNNNNNNISEVGDGSEV 120

Query: 121 NCVVNIECEDQEEEEVRVNKN--GGCECEGEGEKGGMRLLKTEEITKFSTKQIAVLALAI 180
           +CVV I+C   EEE V V+K+  GGCECE +        LKT+E TKF     AVLAL I
Sbjct: 121 HCVVQIQC---EEEVVSVDKDNYGGCECENQEV---AMALKTKE-TKF-----AVLALTI 180

Query: 181 GPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAG 240
           GPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDA +ERQSLTIVN+VAV VS+AG
Sbjct: 181 GPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDAFLERQSLTIVNIVAVVVSLAG 240

Query: 241 VAMTTVGKTWARDEAHSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDM 300
           VAMTT+GKTWA+D+ HS+SS HGKHS+VGD FALLSA TDGLYYVLLKKYAGEEGEK+DM
Sbjct: 241 VAMTTIGKTWAKDQPHSTSSEHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKIDM 300

Query: 301 QKFLGYVGLFTLTTLWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWAM 360
           QKFLGYVGLFTLTTLWWL+WPLRAIGIEPKFAIP+STKVAE+VLANCF+ NF+SDYFWAM
Sbjct: 301 QKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLSDYFWAM 360

Query: 361 GVVWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANLSDWISPK 420
           GVVWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYI GS+QVFLGFMIANLSDWISPK
Sbjct: 361 GVVWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYIFGSLQVFLGFMIANLSDWISPK 410

Query: 421 LKLRKMFFN 428
           LKLR  FFN
Sbjct: 421 LKLRSRFFN 410

BLAST of Lag0034274 vs. TAIR 10
Match: AT4G32140.1 (EamA-like transporter family )

HSP 1 Score: 375.6 bits (963), Expect = 5.5e-104
Identity = 219/423 (51.77%), Postives = 283/423 (66.90%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           M W+YK GL L+  VV+IWV+SAE+TQ IFT Y+ PF VTY+G S+++ YL +AF+K+ +
Sbjct: 1   MGWRYKAGLFLIGTVVIIWVTSAEVTQDIFTAYKQPFAVTYLGASLMIVYLPVAFLKDWL 60

Query: 61  MKLFRSHFRNGNSKKVAEIQPPAIEERTS--QSSSIEEDNNNNNNNNNNNNNISEVENGD 120
            +      R+  S K+  +   +  E  S  +   IE          ++  ++S  E  D
Sbjct: 61  CRYLDR--RSSKSNKIPALTDDSSVELGSPLRHKIIEMGLQGTITKKDSEEHLSSHEEED 120

Query: 121 VDNCVVNIECEDQEEEEVRVNKNGGCECEGEGEKGGMRLLKTEEITKFSTKQIAVLALAI 180
               +  +       EEV+                   L + +EIT   TKQIA+  L +
Sbjct: 121 ERPLIGKV------REEVQT------------------LKQRKEIT---TKQIAMYGLYL 180

Query: 181 GPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAG 240
            PIWFV+EY +NAALARTSVA+TT+L STSGLFTL I   + + +L +  VVAVFVSMAG
Sbjct: 181 APIWFVTEYLSNAALARTSVASTTVLSSTSGLFTLFIGVFLGQDTLNLSKVVAVFVSMAG 240

Query: 241 VAMTTVGKTWARDEAHSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDM 300
           V MTT+GKTWA DE+  +SS +G+ S +GD+F LLSA + GL+ VLLKK+AGEEGE VD+
Sbjct: 241 VVMTTLGKTWASDESQLNSSLNGERSLMGDLFGLLSAVSYGLFTVLLKKFAGEEGEGVDV 300

Query: 301 QKFLGYVGLFTLTTLWWLVWPLRAIGIEPKFAIPQSTKVAEIVLANCFVANFVSDYFWAM 360
           QK  GY+GLFTL  LWWLVWPL A+GIEPKF IP S KV E+VLAN F+ + +SDYFWA+
Sbjct: 301 QKLFGYIGLFTLVALWWLVWPLTALGIEPKFTIPHSVKVDEVVLANGFIGSVLSDYFWAL 360

Query: 361 GVVWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANLSDWISPK 420
            VVWT+PLVA LG SLTIPLAM+ DM++HGRHYS +YILGS QVF GF+IAN+SD  S K
Sbjct: 361 SVVWTTPLVATLGMSLTIPLAMVADMMIHGRHYSAIYILGSTQVFAGFVIANISDLFSKK 394

Query: 421 LKL 422
           L L
Sbjct: 421 LGL 394

BLAST of Lag0034274 vs. TAIR 10
Match: AT3G07080.1 (EamA-like transporter family )

HSP 1 Score: 108.6 bits (270), Expect = 1.3e-23
Identity = 110/415 (26.51%), Postives = 201/415 (48.43%), Query Frame = 0

Query: 3   WKYKGGLILLIAVVVIWVSSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECIMK 62
           W++  GLI L AV  IW++++ + QS+      PF++T++  S+ V YL +  I   +  
Sbjct: 6   WRWVLGLIYLFAVATIWIAASFVVQSVVEAGVSPFLITFICNSLFVVYLPLFEIGRYLED 65

Query: 63  LFRS--HFRNGNSKKVAEIQPPAIEERTSQSSSIEEDNNNNNNNNNNNNNISEVENGDVD 122
            + S   +R+  S  +  ++           S ++ D   ++        ISE  NG ++
Sbjct: 66  AYGSLLFWRSKRSHLMELVESEKAVLLGKDVSGVKSDATESSGLVVREQEISEGGNG-IE 125

Query: 123 NCVVNIECEDQEEEEVRVNKNGGCECEGEGEKGGMRLLKTEEITKFSTKQIAVLALAIGP 182
           + + NIE     E +  VN + G    G   KG       +E  +++  ++A ++L I P
Sbjct: 126 SGLENIEL----EIDASVNVSDG--ASGVSNKG------LDEKGRWTRMRVAKVSLVICP 185

Query: 183 IWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDACMERQSLTIVNVVAVFVSMAGVA 242
            WF+++   N +L  T+V + TIL S S LFT ++      +  T + + +V + M+G  
Sbjct: 186 FWFLAQLTFNVSLKYTTVTSNTILSSASSLFTFLVSLIFLGEKFTWLKLFSVLLCMSGTI 245

Query: 243 MTTVGKTWARDEAHSSSSGHGKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGE---KVD 302
           + ++G      ++ S S+   K+  +GD+ +L+SA    +Y  L++K   ++ E   +V 
Sbjct: 246 IVSMG------DSESKSNAVAKNPLLGDILSLVSAALYAVYITLIRKKLPDDDERNGRVS 305

Query: 303 MQKFLGYVGLFTLTTLWWLVWPLRAIGIEPKFAIPQST-KVAEIVLANCFVANFVSDYFW 362
           M +FLG++GLF     +  + P   +    +      T K   +V+    + N +SDY W
Sbjct: 306 MAQFLGFLGLF---NFFIFLPPALILNFTKRERFNALTLKQFGLVVGKGLLDNVLSDYLW 365

Query: 363 AMGVVWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYILGSVQVFLGFMIANL 412
           A  V+ T+  VA  G ++ +PLA + D  L G   S    +G+  V +GF   N+
Sbjct: 366 AKAVLLTTTTVATAGLTIQVPLAAIVDS-LSGNKPSFTDYIGAAAVMVGFAGINI 397