Lag0034272 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0034272
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionH(+)-exporting diphosphatase
Locationchr3: 5870933 .. 5875602 (-)
RNA-Seq ExpressionLag0034272
SyntenyLag0034272
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCGCCACCATTCTTCCAGATCTCGGGGCTGAGATTTTCATTCCGGTATGTGCCATAGTCGGAATCGTCTTCTCTTTGGTCCAGTGGTATTATGTTTCTCAGGTTAAGCTCTCCCCTGGACGAGATTCCGCTCACAACAACTCTGCCGCTGCCAAGAATGGCTACTCCGACTACCTCATCGAGGAGGAAGAGGGCGTCAACGACCACAACGTCGTTATCAAGTGTGCCGAAATTCAGAGCGCCATTTCCGAGGGTATAACGCTTTCTCTCCTTCTCCTTCCTTCCAGATTTGTTTTCTGCTTTCTTATTTATTGCTAGATCTGCTCCTGGATTCTCAGATTTATCATTCTTAGATTAGATTTTCGTTTTCTCTTTTATTTTGCTTTCCTTTTGTGAACGCCTTAGGGTTTATTTCGTTTTCTCTCCCTCCTCTGTGGTTTTCATTTCTCGTTTCTCTGCCTGGCTCTTGGACTTCTCTAGCGGTTTTTGTTTTTGTTTTTGTTAGATGGTAACGTGTAGTTTGGATTCTTATTTCGAAGTTGTTGCGTGTTGTGTTGTTTTTACGCGAATATCGTGCGTGGTGGTGGGCGTGTGGTTGTTGCATTGGTCCTATCGCTCCATGCATTTCCGGGCCATGCTTCTTGTTTTTTAGCTATTACGTTATGGACTCTGTGTGGCATGAGCATCCATCCCTGCTTTTGACAGTGGGAGCTCGGGTCACTGGTCTTAACGCATTACCATAAAAGGGTTACGGAGCCGTTAATTACGAATTAATTAATTGGTTTGATTGGCTGTCGTGTCAGTACATACATATAGTTTATTGGTTTAGCAAATAAATCTTTTCCTGGGGGTGGGTGATTATGGTCTTTTGTTTATGAAGGATTCTTTCAAGCATCTTCAAATCAATTATGAAAACGTGAATTTATGACGAGATCTAGAAAATGATGACTGAAATAGTTTCAGTTTATTAAATAACAGAGAAGGGCGCACTATTTTGGAACTTATTCTCTTATGTGGGTGTCGTTTTCATCTGTTCGGGTTTGTAGTGCATCTTTTTTGGTTCGAGGATAGAAGTGCATCACCACATCTAGAGTTAATCAGCATTTCTTCCAATTCTCTAAGATTCCTGTTTCCTTGCATCAGGTTCTACGTTGGTCAGTGTCTTGTTTGTTCTGTGTGGTATTTATGTGATTTTTTTTTTGGAAAATACTTATTCGAATAATATTATCAATATAGCATATTTGTTTTGGTTAAAAAATTGGAAACGTTCGCAAAGAAAATATCAAGACAATTCTTTTTTTATTTTTATTTTTGGTTAAAAAATTGGAAACAAATTTATGAGTTCCTTTTGTTGAGGTTTCTACGTTAGTGCCTTCTGGAAAGATTTTTTTTTCTCTGTTATTTGTGAATTCTTTTAATCGAAGATGTCATCTTCCAGAAATTAAAACTCTTAAGCCAATATATTTCTTTTCTTGCAACGAAGAAGTGTAAAGTATTGTGTTGTCTCCACTTAGTAAAATCTGTAGGAATATGAATGGTGTAAGTTGATAATACATACCAGTTGCTTTCCCTAATCGATCAGAATGTTTGTTCTTTCAAATTGTATGGTTATATACTCTCATCTTGAATTGATTTGGACCTGTTTCTAACCTTTTTAACTCTTTGATGTATTAGGAGCAACCTCCTTCCTTTTTACCGAGTACAAGTATGTTGGCATCTTTATGGTGTTGTTTGCAGTCTTGATTTTCGTATTCCTTGGCTCAGTGGAGAGTTTTAGTACCAAGCCTCAAGCATGCACATATGACAAAACAAAGATTTGTAAGCCAGCTTTGGCAACGGCTATATTCAGCACTGTATCATTTTTACTTGGTGCAGTTACCTCAGTTATTTCTGGTTTCCTTGGAATGAAAATTGCTACATATGCAAATGCCAGAACTACCTTGGAAGCAAGAAAGGGTGTTGGAAAGGCATTTATTACTGCTTTTAGGTCTGGTGCTGTCATGGGCTTCCTCCTAGCTGCCAATGGTCTCTTGGTTTTGTTTATTGCTATTAACCTCTTCAAATTGTACTATGGTGATGACTGGGGTGGCCTTTTTGAGTCTATCACTGGGTATGGTCTTGGTGGATCTTCCATGGCTCTCTTTGGTAGAGTTGGTGGTGGTATCTACACAAAAGCTGCTGATGTTGGTGCTGACCTCGTGGGCAAGGTGGAAAGGAACATTCCTGAGGATGATCCAAGAAACCCAGCTGTAATGATCGCTTCTCTAAATATCTTTTCTCAGTTCATTTGTGCTGATACTTTTGTCTCTTGATCTAATATCACTAATTGTTTTGACAGGTGATTGCTGACAATGTTGGTGACAATGTTGGGGATATAGCCGGTATGGGATCTGATCTTTTTGGTTCATATGCTGAATCGTCCTGTGCTGCTCTCGTAGTTGCTTCTATCTCTTCTTTCGGCAACAACCATGAGTTGACACCAATGCTCTATCCTCTCATCGTCAGCTCTATGGGTATCCTTGTTTGCCTGATCACCACCTTATTTGCAACTGATTTCTTTGAGATCAAGGCTGTTAAGGAAATTGAGCCAGCATTGAAGAAGCAACTCATAATTTCCACTGTTCTAATGACCTTTGGAATTGCAATTGTTACTTGGTTGGCTGTTCCATCATCATTCACCATTTTCAACTTTGGAACTCAAAAAGAAGTGCAAAACTGGTTAGTGCGTCTTGAATTATTCTCTTACCATTGCCTTTCATTTTCACTAGCGGTTTGATTCACCAGCAAGTATTTTTGGCATTCAGGAAACTATTCTTGTGTGTTGCTGTTGGTCTCTGGGCTGGGCTTATAATTGGATTTGTGACAGAGTACTATACGAGCAATGCATACAGGTAATTGATTTATTGGCATTTACGTAGTCAATTCACTATGATGCAATTTGTTATATTTTTAAAATTAAAATCTGATTGCATATTTATCTGGTTTTTCAGCCCTGTGCAAGATGTTGCTGATTCTTGCCGAACTGGAGCTGCTACAAATGTCATTTTTGGGTTGGCATTGGGATACAAATCTGTCATTATCCCTATTTTTGCAATTGCGGTTAGCATTTTTGTGAGTTTCACCTTCGCTGCAATGTACGGCATTGCAGTTGCTGCCCTTGGAATGCTGAGTACAATAGCTACTGGTTTGGCCATTGATGCGTATGGTCCAATCAGTGACAATGCTGGAGGCATTGCTGAGATGGCTGGCATGAGCCACAGAATTCGGGAGAGAACCGATGCCCTTGATGCTGCTGGAAACACCACTGCAGCCATTGGAAAGGTTTTTTTTTTTTTTTTTGTTTATTCTTTTCATCTCAATCTTACTGCCTGGATCGATTCTTTATTGGCATTGATTTTGCCTCCAAAGCCTTCCAGTGATTGATTCTCTATTTTTGTCTTTTCCAGGGTTTTGCCATTGGTTCAGCTGCTCTTGTGTCTCTTGCCCTTTTCGGTGCCTTTGTCAGCCGAGCCGGCGTCACTGCTGTTGACGTCTTGACACCCAAAGTTTTCATTGGGTTGATCGTGGGTGCTATGCTCCCATACTGGTTTTCTGCCATGACAATGAAGAGTGTTGGTAGTGCAGCCCTAAAGATGGTTGAGGAGGTGCGAAGGCAATTCAACACCATCCCAGGTCTCATGGAGGGTACCGCCAAGCCCGACTATGCCACATGTGTCAAGATCTCAACTGATGCTTCCATCAAAGAGATGATCCCACCTGGTGCCCTTGTCATGTTGACGCCCCTAATTGTCGGTATCCTATTCGGAGTCGAAACTCTTTCTGGTGTCCTTGCTGGATCTCTTGTTTCTGGTGTGCAGGTATGTAATGGGTAGTAGTTAAATGCTAAACTTTTGCATGGAACCTCTTTATAATACCATCTAACTAACTGGATGTTCTTGTCGTGCACAATTGTAGATTGCTATCTCTGCATCCAACACCGGAGGTGCCTGGGATAACGCCAAGAAGTACATTGAGGTATGTCAAATCCAATAAATCATCTTTACATTGTTGTAATGATTTGATGGTAATTAGATGTAATAGCAAGTTGGTGGTTGATGATTATGGACAGGCTGGTGCCTCTGAGCATGCAAGAACCCTCGGCCCCAAAGGATCAGACCCACACAAAGCGGCTGTGATCGGCGACACAATCGGAGACCCGCTCAAGGATACATCAGGACCTTCCCTGAACATTCTGATAAAGTTGATGGCTGTGGAGTCGCTTGTGTTCGCTCCTTTCTTTGCGAGTCACGGAGGTCTTCTGTTCAAGATCTTCTAGAAAGAAAGCAAGAGGATTTCTGTTCTTTGATTGACATAACCTCTCCTCTTTGTTCTACGAAATTTTCCATGCTTTTTTTTTAGACTTCCATATTATATGTTCCAAGTCTGTAGCAGCTTTGATTCCCTTTGAGAAACTTGGTGTAGTTTCAAGTGGCAGAGCGTTTTTTACGTTTTCATCGGTTGATGATGATGATGATGATGAAGAAGAAGAAAAGAAGATGTGGGTTGAATGTTGTGAAATGAGACACTTGGATGTTATTCTCTTTTTCCCAATTGAGAACAGGCTATTATTGTTACTCTGTCCCCCTTTTCCTCTTCCTCTTCATCTTCCTCTGTTGTTGAATCCTTAG

mRNA sequence

ATGGGCGCCACCATTCTTCCAGATCTCGGGGCTGAGATTTTCATTCCGGTATGTGCCATAGTCGGAATCGTCTTCTCTTTGGTCCAGTGGTATTATGTTTCTCAGGTTAAGCTCTCCCCTGGACGAGATTCCGCTCACAACAACTCTGCCGCTGCCAAGAATGGCTACTCCGACTACCTCATCGAGGAGGAAGAGGGCGTCAACGACCACAACGTCGTTATCAAGTGTGCCGAAATTCAGAGCGCCATTTCCGAGGGAGCAACCTCCTTCCTTTTTACCGAGTACAAGTATGTTGGCATCTTTATGGTGTTGTTTGCAGTCTTGATTTTCGTATTCCTTGGCTCAGTGGAGAGTTTTAGTACCAAGCCTCAAGCATGCACATATGACAAAACAAAGATTTGTAAGCCAGCTTTGGCAACGGCTATATTCAGCACTGTATCATTTTTACTTGGTGCAGTTACCTCAGTTATTTCTGGTTTCCTTGGAATGAAAATTGCTACATATGCAAATGCCAGAACTACCTTGGAAGCAAGAAAGGGTGTTGGAAAGGCATTTATTACTGCTTTTAGGTCTGGTGCTGTCATGGGCTTCCTCCTAGCTGCCAATGGTCTCTTGGTTTTGTTTATTGCTATTAACCTCTTCAAATTGTACTATGGTGATGACTGGGGTGGCCTTTTTGAGTCTATCACTGGGTATGGTCTTGGTGGATCTTCCATGGCTCTCTTTGGTAGAGTTGGTGGTGGTATCTACACAAAAGCTGCTGATGTTGGTGCTGACCTCGTGGGCAAGGTGGAAAGGAACATTCCTGAGGATGATCCAAGAAACCCAGCTGTGATTGCTGACAATGTTGGTGACAATGTTGGGGATATAGCCGGTATGGGATCTGATCTTTTTGGTTCATATGCTGAATCGTCCTGTGCTGCTCTCGTAGTTGCTTCTATCTCTTCTTTCGGCAACAACCATGAGTTGACACCAATGCTCTATCCTCTCATCGTCAGCTCTATGGGTATCCTTGTTTGCCTGATCACCACCTTATTTGCAACTGATTTCTTTGAGATCAAGGCTGTTAAGGAAATTGAGCCAGCATTGAAGAAGCAACTCATAATTTCCACTGTTCTAATGACCTTTGGAATTGCAATTGTTACTTGGTTGGCTGTTCCATCATCATTCACCATTTTCAACTTTGGAACTCAAAAAGAAGTGCAAAACTGGAAACTATTCTTGTGTGTTGCTGTTGGTCTCTGGGCTGGGCTTATAATTGGATTTGTGACAGAGTACTATACGAGCAATGCATACAGCCCTGTGCAAGATGTTGCTGATTCTTGCCGAACTGGAGCTGCTACAAATGTCATTTTTGGGTTGGCATTGGGATACAAATCTGTCATTATCCCTATTTTTGCAATTGCGGTTAGCATTTTTGTGAGTTTCACCTTCGCTGCAATGTACGGCATTGCAGTTGCTGCCCTTGGAATGCTGAGTACAATAGCTACTGGTTTGGCCATTGATGCGTATGGTCCAATCAGTGACAATGCTGGAGGCATTGCTGAGATGGCTGGCATGAGCCACAGAATTCGGGAGAGAACCGATGCCCTTGATGCTGCTGGAAACACCACTGCAGCCATTGGAAAGGGTTTTGCCATTGGTTCAGCTGCTCTTGTGTCTCTTGCCCTTTTCGGTGCCTTTGTCAGCCGAGCCGGCGTCACTGCTGTTGACGTCTTGACACCCAAAGTTTTCATTGGGTTGATCGTGGGTGCTATGCTCCCATACTGGTTTTCTGCCATGACAATGAAGAGTGTTGGTAGTGCAGCCCTAAAGATGGTTGAGGAGGTGCGAAGGCAATTCAACACCATCCCAGGTCTCATGGAGGGTACCGCCAAGCCCGACTATGCCACATGTGTCAAGATCTCAACTGATGCTTCCATCAAAGAGATGATCCCACCTGGTGCCCTTGTCATGTTGACGCCCCTAATTGTCGGTATCCTATTCGGAGTCGAAACTCTTTCTGGTGTCCTTGCTGGATCTCTTGTTTCTGGTGTGCAGATTGCTATCTCTGCATCCAACACCGGAGGTGCCTGGGATAACGCCAAGAAGTACATTGAGGCTGGTGCCTCTGAGCATGCAAGAACCCTCGGCCCCAAAGGATCAGACCCACACAAAGCGGCTGTGATCGGCGACACAATCGGAGACCCGCTCAAGGATACATCAGGACCTTCCCTGAACATTCTGATAAAGTTGATGGCTGTGGAGTCGCTTGTGTTCGCTCCTTTCTTTGCGAGTCACGGAGACTTCCATATTATATGTTCCAAGTCTGTAGCAGCTTTGATTCCCTTTGAGAAACTTGGTGTAGTTTCAAGTGGCAGAGCGTTTTTTACGTTTTCATCGGTTGATGATGATGATGATGATGAAGAAGAAGAAAAGAAGATGTGGGTTGAATGTTGTGAAATGAGACACTTGGATGTTATTCTCTTTTTCCCAATTGAGAACAGGCTATTATTGTTACTCTGTCCCCCTTTTCCTCTTCCTCTTCATCTTCCTCTGTTGTTGAATCCTTAG

Coding sequence (CDS)

ATGGGCGCCACCATTCTTCCAGATCTCGGGGCTGAGATTTTCATTCCGGTATGTGCCATAGTCGGAATCGTCTTCTCTTTGGTCCAGTGGTATTATGTTTCTCAGGTTAAGCTCTCCCCTGGACGAGATTCCGCTCACAACAACTCTGCCGCTGCCAAGAATGGCTACTCCGACTACCTCATCGAGGAGGAAGAGGGCGTCAACGACCACAACGTCGTTATCAAGTGTGCCGAAATTCAGAGCGCCATTTCCGAGGGAGCAACCTCCTTCCTTTTTACCGAGTACAAGTATGTTGGCATCTTTATGGTGTTGTTTGCAGTCTTGATTTTCGTATTCCTTGGCTCAGTGGAGAGTTTTAGTACCAAGCCTCAAGCATGCACATATGACAAAACAAAGATTTGTAAGCCAGCTTTGGCAACGGCTATATTCAGCACTGTATCATTTTTACTTGGTGCAGTTACCTCAGTTATTTCTGGTTTCCTTGGAATGAAAATTGCTACATATGCAAATGCCAGAACTACCTTGGAAGCAAGAAAGGGTGTTGGAAAGGCATTTATTACTGCTTTTAGGTCTGGTGCTGTCATGGGCTTCCTCCTAGCTGCCAATGGTCTCTTGGTTTTGTTTATTGCTATTAACCTCTTCAAATTGTACTATGGTGATGACTGGGGTGGCCTTTTTGAGTCTATCACTGGGTATGGTCTTGGTGGATCTTCCATGGCTCTCTTTGGTAGAGTTGGTGGTGGTATCTACACAAAAGCTGCTGATGTTGGTGCTGACCTCGTGGGCAAGGTGGAAAGGAACATTCCTGAGGATGATCCAAGAAACCCAGCTGTGATTGCTGACAATGTTGGTGACAATGTTGGGGATATAGCCGGTATGGGATCTGATCTTTTTGGTTCATATGCTGAATCGTCCTGTGCTGCTCTCGTAGTTGCTTCTATCTCTTCTTTCGGCAACAACCATGAGTTGACACCAATGCTCTATCCTCTCATCGTCAGCTCTATGGGTATCCTTGTTTGCCTGATCACCACCTTATTTGCAACTGATTTCTTTGAGATCAAGGCTGTTAAGGAAATTGAGCCAGCATTGAAGAAGCAACTCATAATTTCCACTGTTCTAATGACCTTTGGAATTGCAATTGTTACTTGGTTGGCTGTTCCATCATCATTCACCATTTTCAACTTTGGAACTCAAAAAGAAGTGCAAAACTGGAAACTATTCTTGTGTGTTGCTGTTGGTCTCTGGGCTGGGCTTATAATTGGATTTGTGACAGAGTACTATACGAGCAATGCATACAGCCCTGTGCAAGATGTTGCTGATTCTTGCCGAACTGGAGCTGCTACAAATGTCATTTTTGGGTTGGCATTGGGATACAAATCTGTCATTATCCCTATTTTTGCAATTGCGGTTAGCATTTTTGTGAGTTTCACCTTCGCTGCAATGTACGGCATTGCAGTTGCTGCCCTTGGAATGCTGAGTACAATAGCTACTGGTTTGGCCATTGATGCGTATGGTCCAATCAGTGACAATGCTGGAGGCATTGCTGAGATGGCTGGCATGAGCCACAGAATTCGGGAGAGAACCGATGCCCTTGATGCTGCTGGAAACACCACTGCAGCCATTGGAAAGGGTTTTGCCATTGGTTCAGCTGCTCTTGTGTCTCTTGCCCTTTTCGGTGCCTTTGTCAGCCGAGCCGGCGTCACTGCTGTTGACGTCTTGACACCCAAAGTTTTCATTGGGTTGATCGTGGGTGCTATGCTCCCATACTGGTTTTCTGCCATGACAATGAAGAGTGTTGGTAGTGCAGCCCTAAAGATGGTTGAGGAGGTGCGAAGGCAATTCAACACCATCCCAGGTCTCATGGAGGGTACCGCCAAGCCCGACTATGCCACATGTGTCAAGATCTCAACTGATGCTTCCATCAAAGAGATGATCCCACCTGGTGCCCTTGTCATGTTGACGCCCCTAATTGTCGGTATCCTATTCGGAGTCGAAACTCTTTCTGGTGTCCTTGCTGGATCTCTTGTTTCTGGTGTGCAGATTGCTATCTCTGCATCCAACACCGGAGGTGCCTGGGATAACGCCAAGAAGTACATTGAGGCTGGTGCCTCTGAGCATGCAAGAACCCTCGGCCCCAAAGGATCAGACCCACACAAAGCGGCTGTGATCGGCGACACAATCGGAGACCCGCTCAAGGATACATCAGGACCTTCCCTGAACATTCTGATAAAGTTGATGGCTGTGGAGTCGCTTGTGTTCGCTCCTTTCTTTGCGAGTCACGGAGACTTCCATATTATATGTTCCAAGTCTGTAGCAGCTTTGATTCCCTTTGAGAAACTTGGTGTAGTTTCAAGTGGCAGAGCGTTTTTTACGTTTTCATCGGTTGATGATGATGATGATGATGAAGAAGAAGAAAAGAAGATGTGGGTTGAATGTTGTGAAATGAGACACTTGGATGTTATTCTCTTTTTCCCAATTGAGAACAGGCTATTATTGTTACTCTGTCCCCCTTTTCCTCTTCCTCTTCATCTTCCTCTGTTGTTGAATCCTTAG

Protein sequence

MGATILPDLGAEIFIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSAAAKNGYSDYLIEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFSTKPQACTYDKTKICKPALATAIFSTVSFLLGAVTSVISGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPALKKQLIISTVLMTFGIAIVTWLAVPSSFTIFNFGTQKEVQNWKLFLCVAVGLWAGLIIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGDFHIICSKSVAALIPFEKLGVVSSGRAFFTFSSVDDDDDDEEEEKKMWVECCEMRHLDVILFFPIENRLLLLLCPPFPLPLHLPLLLNP
Homology
BLAST of Lag0034272 vs. NCBI nr
Match: XP_022929683.1 (pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita moschata] >KAG6587868.1 hypothetical protein SDJN03_16433, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021755.1 hypothetical protein SDJN02_15482 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1411.7 bits (3653), Expect = 0.0e+00
Identity = 747/761 (98.16%), Postives = 753/761 (98.95%), Query Frame = 0

Query: 1   MGATILPDLGAEIFIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSAAAKNGYSDYL 60
           M  TILPDLG EIFIPVCA+VGIVFSLVQWYYVSQVKLSPGRD+AHNNSA +KNGYSDYL
Sbjct: 1   MSVTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120
           IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120

Query: 121 TKPQACTYDKTKICKPALATAIFSTVSFLLGAVTSVISGFLGMKIATYANARTTLEARKG 180
           TKPQ CTYDKT+ CKPALATAIFSTVSFLLGAVTSV+SGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PALKKQLIISTVLMTFGIAIVTWLAVPSSFTIFNFGTQKEVQNWKLFLCVAVGLWAGLII 420
           PALKKQLIISTVLMTFGIAIVTWL+VPSSFTIFNFGTQK V NWKLFLCVAVGLWAGLII
Sbjct: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG 762
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG 761

BLAST of Lag0034272 vs. NCBI nr
Match: XP_023002108.1 (pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita maxima])

HSP 1 Score: 1411.0 bits (3651), Expect = 0.0e+00
Identity = 745/761 (97.90%), Postives = 753/761 (98.95%), Query Frame = 0

Query: 1   MGATILPDLGAEIFIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSAAAKNGYSDYL 60
           M  TILPDLG EIFIPVCA++GI+FSLVQWYYVSQVKLSPGRD+AHNNSA +KNGYSDYL
Sbjct: 1   MSVTILPDLGTEIFIPVCAVIGIIFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120
           IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120

Query: 121 TKPQACTYDKTKICKPALATAIFSTVSFLLGAVTSVISGFLGMKIATYANARTTLEARKG 180
           TKPQ CTYDKT+ CKPALATAIFSTVSFLLGAVTSV+SGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PALKKQLIISTVLMTFGIAIVTWLAVPSSFTIFNFGTQKEVQNWKLFLCVAVGLWAGLII 420
           PALKKQLIISTVLMTFGIAIVTWL+VPSSFTIFNFGTQK V NWKLFLCVAVGLWAGLII
Sbjct: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG 762
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG 761

BLAST of Lag0034272 vs. NCBI nr
Match: XP_023529940.1 (pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1409.4 bits (3647), Expect = 0.0e+00
Identity = 745/761 (97.90%), Postives = 752/761 (98.82%), Query Frame = 0

Query: 1   MGATILPDLGAEIFIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSAAAKNGYSDYL 60
           M  TILPDLG EIFIPVCA+VGIVFSLVQWYYVSQVKLSPGRD+AHNNSA +KNGYSDYL
Sbjct: 1   MSVTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120
           IEEEEGVNDHNVVIKCAEIQ AISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQCAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120

Query: 121 TKPQACTYDKTKICKPALATAIFSTVSFLLGAVTSVISGFLGMKIATYANARTTLEARKG 180
           TKPQ CTYDKT+ CKPALATAIFSTVSFLLGAVTSV+SGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHELTPMLYPLI+SSMGILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIISSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PALKKQLIISTVLMTFGIAIVTWLAVPSSFTIFNFGTQKEVQNWKLFLCVAVGLWAGLII 420
           PALKKQLIISTVLMTFGIAIVTWL+VPSSFTIFNFGTQK V NWKLFLCVAVGLWAGLII
Sbjct: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG 762
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG 761

BLAST of Lag0034272 vs. NCBI nr
Match: BAA33149.1 (proton-translocating inorganic pyrophosphatase [Cucurbita moschata])

HSP 1 Score: 1407.9 bits (3643), Expect = 0.0e+00
Identity = 744/761 (97.77%), Postives = 752/761 (98.82%), Query Frame = 0

Query: 1   MGATILPDLGAEIFIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSAAAKNGYSDYL 60
           M  TILPDLG EIFIPVCA++GIVFSLVQWYYVSQVKLSPGRD+AHNNSA +KNGYSDYL
Sbjct: 1   MSVTILPDLGTEIFIPVCAVIGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120
           IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120

Query: 121 TKPQACTYDKTKICKPALATAIFSTVSFLLGAVTSVISGFLGMKIATYANARTTLEARKG 180
           TKPQ CTYDKT+ CKPALATAIFSTVSFLLGAVTSV+SGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASIS FGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISPFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PALKKQLIISTVLMTFGIAIVTWLAVPSSFTIFNFGTQKEVQNWKLFLCVAVGLWAGLII 420
           PALKKQLIISTVLMTFGIAIVTWL+VPSSFTIFNFGTQK V NWKLFLCVAVGLWAGLII
Sbjct: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGAS+HARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASKHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG 762
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG 761

BLAST of Lag0034272 vs. NCBI nr
Match: XP_023515840.1 (pyrophosphate-energized vacuolar membrane proton pump [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1406.0 bits (3638), Expect = 0.0e+00
Identity = 741/758 (97.76%), Postives = 754/758 (99.47%), Query Frame = 0

Query: 4   TILPDLGAEIFIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSAAAKNGYSDYLIEE 63
           TILPDLG +IFIPVCA+VGIVFSLVQWYYVSQVKLSPGRDS HNNSAAAKNGYSDYLIEE
Sbjct: 5   TILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDSTHNNSAAAKNGYSDYLIEE 64

Query: 64  EEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFSTKP 123
           EEGVNDHNVVIKCAEIQ+AISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVE FST+P
Sbjct: 65  EEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGFSTEP 124

Query: 124 QACTYDKTKICKPALATAIFSTVSFLLGAVTSVISGFLGMKIATYANARTTLEARKGVGK 183
           QACTYDKTKICKPALATAIFST+SFLLGAVTSV+SGFLGMKIATYANARTTLEARKGVGK
Sbjct: 125 QACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGK 184

Query: 184 AFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALFG 243
           AFITAFRSGAVMGFLLAANGLLVLFIAIN+FKLYYG+DWGGLFESITGYGLGGSSMALFG
Sbjct: 185 AFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSMALFG 244

Query: 244 RVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 303
           RVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE
Sbjct: 245 RVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 304

Query: 304 SSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPAL 363
           SSCAALVVASISSFGNNHELTPMLYPLIVSS+GILVCLITTLFATDFFEIKAVKEIEPAL
Sbjct: 305 SSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEIEPAL 364

Query: 364 KKQLIISTVLMTFGIAIVTWLAVPSSFTIFNFGTQKEVQNWKLFLCVAVGLWAGLIIGFV 423
           KKQLIISTVLMTFGIAIVTW+AVPS+FTIFNFGTQK VQNWKLFLCVAVGLWAGLIIGFV
Sbjct: 365 KKQLIISTVLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLIIGFV 424

Query: 424 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYG 483
           TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYG
Sbjct: 425 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYG 484

Query: 484 IAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 543
           IAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK
Sbjct: 485 IAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 544

Query: 544 GFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAA 603
           GFAIGSAALVSLALFGAFVSRAGVT+VDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAA
Sbjct: 545 GFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAA 604

Query: 604 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFG 663
           LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFG
Sbjct: 605 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFG 664

Query: 664 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVI 723
           VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVI
Sbjct: 665 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVI 724

Query: 724 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG 762
           GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HG
Sbjct: 725 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHG 762

BLAST of Lag0034272 vs. ExPASy Swiss-Prot
Match: P21616 (Pyrophosphate-energized vacuolar membrane proton pump OS=Vigna radiata var. radiata OX=3916 PE=1 SV=4)

HSP 1 Score: 1332.4 bits (3447), Expect = 0.0e+00
Identity = 700/761 (91.98%), Postives = 737/761 (96.85%), Query Frame = 0

Query: 1   MGATILPDLGAEIFIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSAAAKNGYSDYL 60
           MGA ILPDLG EI IPVCA++GI F+L QW  VS+VKLS  RD++ N  AAAKNGY+DYL
Sbjct: 1   MGAAILPDLGTEILIPVCAVIGIAFALFQWLLVSKVKLSAVRDASPN--AAAKNGYNDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120
           IEEEEG+NDHNVV+KCAEIQ+AISEGATSFLFTEYKYVGIFMV FA+LIF+FLGSVE FS
Sbjct: 61  IEEEEGINDHNVVVKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAILIFLFLGSVEGFS 120

Query: 121 TKPQACTYDKTKICKPALATAIFSTVSFLLGAVTSVISGFLGMKIATYANARTTLEARKG 180
           T PQAC+YDKTK CKPALATAIFSTVSFLLG VTS++SGFLGMKIATYANARTTLEARKG
Sbjct: 121 TSPQACSYDKTKTCKPALATAIFSTVSFLLGGVTSLVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVL+IAINLFK+YYGDDWGGLFE+ITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLYIAINLFKIYYGDDWGGLFEAITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFG NHELT MLYPLIVSS+GILVCL+TTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGLNHELTAMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKEIE 360

Query: 361 PALKKQLIISTVLMTFGIAIVTWLAVPSSFTIFNFGTQKEVQNWKLFLCVAVGLWAGLII 420
           PALKKQL+ISTVLMT G+A+V+++A+P+SFTIFNFG QK+V++W+LFLCVAVGLWAGLII
Sbjct: 361 PALKKQLVISTVLMTIGVAVVSFVALPTSFTIFNFGVQKDVKSWQLFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA+SIFVSFTFAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFTFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRA +T VDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRASITTVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPL+VGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLVVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHAR+LGPKGSD HKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARSLGPKGSDCHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG 762
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HG
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHG 759

BLAST of Lag0034272 vs. ExPASy Swiss-Prot
Match: P31414 (Pyrophosphate-energized vacuolar membrane proton pump 1 OS=Arabidopsis thaliana OX=3702 GN=AVP1 PE=1 SV=1)

HSP 1 Score: 1268.4 bits (3281), Expect = 0.0e+00
Identity = 662/758 (87.34%), Postives = 710/758 (93.67%), Query Frame = 0

Query: 5   ILPDLGAEIFIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSA-AAKNGYSDYLIEE 64
           +LP+L  EI +P+CA++GI FSL QWY VS+VKL+    ++ +  A   KNGY DYLIEE
Sbjct: 6   LLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGDYLIEE 65

Query: 65  EEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFSTKP 124
           EEGVND +VV KCAEIQ+AISEGATSFLFTEYKYVG+FM+ FA +IFVFLGSVE FST  
Sbjct: 66  EEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTDN 125

Query: 125 QACTYDKTKICKPALATAIFSTVSFLLGAVTSVISGFLGMKIATYANARTTLEARKGVGK 184
           + CTYD T+ CKPALATA FST++F+LGAVTSV+SGFLGMKIATYANARTTLEARKGVGK
Sbjct: 126 KPCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVGK 185

Query: 185 AFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALFG 244
           AFI AFRSGAVMGFLLAA+GLLVL+I IN+FK+YYGDDW GLFE+ITGYGLGGSSMALFG
Sbjct: 186 AFIVAFRSGAVMGFLLAASGLLVLYITINVFKIYYGDDWEGLFEAITGYGLGGSSMALFG 245

Query: 245 RVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 304
           RVGGGIYTKAADVGADLVGK+ERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE
Sbjct: 246 RVGGGIYTKAADVGADLVGKIERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 305

Query: 305 SSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPAL 364
           +SCAALVVASISSFG NH+ T M YPL++SSMGILVCLITTLFATDFFEIK VKEIEPAL
Sbjct: 306 ASCAALVVASISSFGINHDFTAMCYPLLISSMGILVCLITTLFATDFFEIKLVKEIEPAL 365

Query: 365 KKQLIISTVLMTFGIAIVTWLAVPSSFTIFNFGTQKEVQNWKLFLCVAVGLWAGLIIGFV 424
           K QLIISTV+MT GIAIV+W+ +P+SFTIFNFGTQK V+NW+LFLCV VGLWAGLIIGFV
Sbjct: 366 KNQLIISTVIMTVGIAIVSWVGLPTSFTIFNFGTQKVVKNWQLFLCVCVGLWAGLIIGFV 425

Query: 425 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYG 484
           TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA+SIFVSF+FAAMYG
Sbjct: 426 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFSFAAMYG 485

Query: 485 IAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 544
           +AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK
Sbjct: 486 VAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 545

Query: 545 GFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAA 604
           GFAIGSAALVSLALFGAFVSRAG+  VDVLTPKV IGL+VGAMLPYWFSAMTMKSVGSAA
Sbjct: 546 GFAIGSAALVSLALFGAFVSRAGIHTVDVLTPKVIIGLLVGAMLPYWFSAMTMKSVGSAA 605

Query: 605 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFG 664
           LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPG LVMLTPLIVG  FG
Sbjct: 606 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGCLVMLTPLIVGFFFG 665

Query: 665 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVI 724
           VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAG SEHA++LGPKGS+PHKAAVI
Sbjct: 666 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGVSEHAKSLGPKGSEPHKAAVI 725

Query: 725 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG 762
           GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HG
Sbjct: 726 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHG 763

BLAST of Lag0034272 vs. ExPASy Swiss-Prot
Match: Q06572 (Pyrophosphate-energized vacuolar membrane proton pump OS=Hordeum vulgare OX=4513 PE=2 SV=2)

HSP 1 Score: 1261.9 bits (3264), Expect = 0.0e+00
Identity = 662/757 (87.45%), Postives = 712/757 (94.06%), Query Frame = 0

Query: 5   ILPDLGAEIFIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSAAAKNGYSDYLIEEE 64
           IL +LG EI IPVC ++GIVF++ QW+ VS+VK++PG  SA   +A AKNGY DYLIEEE
Sbjct: 3   ILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPGAASA---AAGAKNGYGDYLIEEE 62

Query: 65  EGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFSTKPQ 124
           EG+NDHNVV+KCAEIQ+AISEGATSFLFT Y+YVG+FMV+FA +IF+FLGS+E FSTK Q
Sbjct: 63  EGLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFSTKGQ 122

Query: 125 ACTYDKTKICKPALATAIFSTVSFLLGAVTSVISGFLGMKIATYANARTTLEARKGVGKA 184
            CTY K   CKPAL TA+FST SFLLGA+TS++SGFLGMKIATYANARTTLEARKGVGKA
Sbjct: 123 PCTYSK-GTCKPALYTALFSTASFLLGAITSLVSGFLGMKIATYANARTTLEARKGVGKA 182

Query: 185 FITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALFGR 244
           FITAFRSGAVMGFLL+++GL+VL+I IN+FK+YYGDDW GLFESITGYGLGGSSMALFGR
Sbjct: 183 FITAFRSGAVMGFLLSSSGLVVLYITINVFKMYYGDDWEGLFESITGYGLGGSSMALFGR 242

Query: 245 VGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAES 304
           VGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAES
Sbjct: 243 VGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAES 302

Query: 305 SCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPALK 364
           SCAALVVASISSFG NH+ T M YPL+VSS+GI+VCL+TTLFATDFFEIKA  EIEPALK
Sbjct: 303 SCAALVVASISSFGINHDFTAMCYPLLVSSVGIIVCLLTTLFATDFFEIKAANEIEPALK 362

Query: 365 KQLIISTVLMTFGIAIVTWLAVPSSFTIFNFGTQKEVQNWKLFLCVAVGLWAGLIIGFVT 424
           KQLIIST LMT G+A+++WLA+P+ FTIFNFG QKEV NW LF CVAVGLWAGLIIGFVT
Sbjct: 363 KQLIISTALMTVGVAVISWLALPAKFTIFNFGAQKEVSNWGLFFCVAVGLWAGLIIGFVT 422

Query: 425 EYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYGI 484
           EYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSI+VSF+ AAMYGI
Sbjct: 423 EYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIYVSFSIAAMYGI 482

Query: 485 AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKG 544
           A+AALGMLST+ATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKG
Sbjct: 483 AMAALGMLSTMATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKG 542

Query: 545 FAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAAL 604
           FAIGSAALVSLALFGAFVSRAGV  VDVL+PKVFIGLIVGAMLPYWFSAMTMKSVGSAAL
Sbjct: 543 FAIGSAALVSLALFGAFVSRAGVKVVDVLSPKVFIGLIVGAMLPYWFSAMTMKSVGSAAL 602

Query: 605 KMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFGV 664
           KMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVG LFGV
Sbjct: 603 KMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGTLFGV 662

Query: 665 ETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIG 724
           ETLSGVLAG+LVSGVQIAISASNTGGAWDNAKKYIEAG SEHAR+LGPKGSD HKAAVIG
Sbjct: 663 ETLSGVLAGALVSGVQIAISASNTGGAWDNAKKYIEAGNSEHARSLGPKGSDCHKAAVIG 722

Query: 725 DTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG 762
           DTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA++G
Sbjct: 723 DTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATYG 755

BLAST of Lag0034272 vs. ExPASy Swiss-Prot
Match: Q72Q29 (Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain Fiocruz L1-130) OX=267671 GN=hppA PE=3 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 8.2e-197
Identity = 392/690 (56.81%), Postives = 498/690 (72.17%), Query Frame = 0

Query: 75  KCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFSTKPQACTYDKTKIC 134
           K  EI SAISEGA +FL  EYK + +F+   AVLI + L +  S                
Sbjct: 42  KLLEISSAISEGAMAFLVREYKVISLFIAFMAVLIVLLLDNPGS---------------- 101

Query: 135 KPALATAIFSTVSFLLGAVTSVISGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAV 194
                  I++ ++F+ GA+ S ISGF+GMKIAT  N RT   A+  + KAF  AF SGAV
Sbjct: 102 -EGFNDGIYTAIAFVSGALISCISGFIGMKIATAGNVRTAEAAKSSMAKAFRVAFDSGAV 161

Query: 195 MGF---LLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALFGRVGGGIYT 254
           MGF    LA  G++VLF+      +Y G +   L ES+ G+GLGGS++ALFGRVGGGIYT
Sbjct: 162 MGFGLVGLAILGMIVLFLVFT--GMYPGVEKHFLMESLAGFGLGGSAVALFGRVGGGIYT 221

Query: 255 KAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALVV 314
           KAADVGADLVGKVE+ IPEDDPRNPA IADNVGDNVGD+AGMG+DLFGS AE++CAALV+
Sbjct: 222 KAADVGADLVGKVEKGIPEDDPRNPATIADNVGDNVGDVAGMGADLFGSCAEATCAALVI 281

Query: 315 ASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPALKKQLIIST 374
            + +S   +  +  +LYPL++S+ GI   ++T+  A     +K    +E ALK QL +ST
Sbjct: 282 GATAS-ALSGSVDALLYPLLISAFGIPASILTSFLA----RVKEDGNVESALKVQLWVST 341

Query: 375 VLMTFGIAIVTWLAVPSSFTIFNFGTQKEVQNWKLFLCVAVGLWAGLIIGFVTEYYTSNA 434
           +L+   +  VT   +  SF I      K +  W +++ + VGL++G+ IG VTEYYTS++
Sbjct: 342 LLVAGIMYFVTKTFMVDSFEI----AGKTITKWDVYISMVVGLFSGMFIGIVTEYYTSHS 401

Query: 435 YSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYGIAVAALGM 494
           Y PV++VA++  TGAATN+I+GL+LGY S +IP+  + ++I  +   A MYGIA+AALGM
Sbjct: 402 YKPVREVAEASNTGAATNIIYGLSLGYHSSVIPVILLVITIVTANLLAGMYGIAIAALGM 461

Query: 495 LSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKGFAIGSAA 554
           +STIA GL IDAYGP+SDNAGGIAEMA +   +R+RTD LDAAGNTTAAIGKGFAIGSAA
Sbjct: 462 ISTIAIGLTIDAYGPVSDNAGGIAEMAELGKEVRDRTDTLDAAGNTTAAIGKGFAIGSAA 521

Query: 555 LVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEVR 614
           L SLALF AF++R   T+++VL  +VF GL+ GAMLP+ F+AMTMKSVG AA+ MVEEVR
Sbjct: 522 LTSLALFAAFITRTHTTSLEVLNAEVFGGLMFGAMLPFLFTAMTMKSVGKAAVDMVEEVR 581

Query: 615 RQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFGVETLSGVL 674
           +QF  IPG+MEG  KPDY  CV IST A+++EMI PG LV+LTP++VG LFGV+TL+GVL
Sbjct: 582 KQFKEIPGIMEGKNKPDYKRCVDISTSAALREMILPGLLVLLTPILVGYLFGVKTLAGVL 641

Query: 675 AGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPL 734
           AG+LV+GV +AISA+N+GG WDNAKKYIE  A       G KGSD HKAAV+GDT+GDP 
Sbjct: 642 AGALVAGVVLAISAANSGGGWDNAKKYIEKKA-------GGKGSDQHKAAVVGDTVGDPF 696

Query: 735 KDTSGPSLNILIKLMAVESLVFAPFFASHG 762
           KDTSGPS+NILIKLMA+ SLVFA FF   G
Sbjct: 702 KDTSGPSINILIKLMAITSLVFAEFFVQQG 696

BLAST of Lag0034272 vs. ExPASy Swiss-Prot
Match: Q8F641 (Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) OX=189518 GN=hppA PE=3 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 8.2e-197
Identity = 392/690 (56.81%), Postives = 498/690 (72.17%), Query Frame = 0

Query: 75  KCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFSTKPQACTYDKTKIC 134
           K  EI SAISEGA +FL  EYK + +F+   AVLI + L +  S                
Sbjct: 42  KLLEISSAISEGAMAFLVREYKVISLFIAFMAVLIVLLLDNPGS---------------- 101

Query: 135 KPALATAIFSTVSFLLGAVTSVISGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAV 194
                  I++ ++F+ GA+ S ISGF+GMKIAT  N RT   A+  + KAF  AF SGAV
Sbjct: 102 -EGFNDGIYTAIAFVSGALISCISGFIGMKIATAGNVRTAEAAKSSMAKAFRVAFDSGAV 161

Query: 195 MGF---LLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALFGRVGGGIYT 254
           MGF    LA  G++VLF+      +Y G +   L ES+ G+GLGGS++ALFGRVGGGIYT
Sbjct: 162 MGFGLVGLAILGMIVLFLVFT--GMYPGVEKHFLMESLAGFGLGGSAVALFGRVGGGIYT 221

Query: 255 KAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALVV 314
           KAADVGADLVGKVE+ IPEDDPRNPA IADNVGDNVGD+AGMG+DLFGS AE++CAALV+
Sbjct: 222 KAADVGADLVGKVEKGIPEDDPRNPATIADNVGDNVGDVAGMGADLFGSCAEATCAALVI 281

Query: 315 ASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPALKKQLIIST 374
            + +S   +  +  +LYPL++S+ GI   ++T+  A     +K    +E ALK QL +ST
Sbjct: 282 GATAS-ALSGSVDALLYPLLISAFGIPASILTSFLA----RVKEDGNVESALKVQLWVST 341

Query: 375 VLMTFGIAIVTWLAVPSSFTIFNFGTQKEVQNWKLFLCVAVGLWAGLIIGFVTEYYTSNA 434
           +L+   +  VT   +  SF I      K +  W +++ + VGL++G+ IG VTEYYTS++
Sbjct: 342 LLVAGIMYFVTKTFMVDSFEI----AGKTITKWDVYISMVVGLFSGMFIGIVTEYYTSHS 401

Query: 435 YSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYGIAVAALGM 494
           Y PV++VA++  TGAATN+I+GL+LGY S +IP+  + ++I  +   A MYGIA+AALGM
Sbjct: 402 YKPVREVAEASNTGAATNIIYGLSLGYHSSVIPVILLVITIVTANLLAGMYGIAIAALGM 461

Query: 495 LSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKGFAIGSAA 554
           +STIA GL IDAYGP+SDNAGGIAEMA +   +R+RTD LDAAGNTTAAIGKGFAIGSAA
Sbjct: 462 ISTIAIGLTIDAYGPVSDNAGGIAEMAELGKEVRDRTDTLDAAGNTTAAIGKGFAIGSAA 521

Query: 555 LVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEVR 614
           L SLALF AF++R   T+++VL  +VF GL+ GAMLP+ F+AMTMKSVG AA+ MVEEVR
Sbjct: 522 LTSLALFAAFITRTHTTSLEVLNAEVFGGLMFGAMLPFLFTAMTMKSVGKAAVDMVEEVR 581

Query: 615 RQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFGVETLSGVL 674
           +QF  IPG+MEG  KPDY  CV IST A+++EMI PG LV+LTP++VG LFGV+TL+GVL
Sbjct: 582 KQFKEIPGIMEGKNKPDYKRCVDISTSAALREMILPGLLVLLTPILVGYLFGVKTLAGVL 641

Query: 675 AGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPL 734
           AG+LV+GV +AISA+N+GG WDNAKKYIE  A       G KGSD HKAAV+GDT+GDP 
Sbjct: 642 AGALVAGVVLAISAANSGGGWDNAKKYIEKKA-------GGKGSDQHKAAVVGDTVGDPF 696

Query: 735 KDTSGPSLNILIKLMAVESLVFAPFFASHG 762
           KDTSGPS+NILIKLMA+ SLVFA FF   G
Sbjct: 702 KDTSGPSINILIKLMAITSLVFAEFFVQQG 696

BLAST of Lag0034272 vs. ExPASy TrEMBL
Match: A0A6J1ESX2 (H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=LOC111436191 PE=3 SV=1)

HSP 1 Score: 1411.7 bits (3653), Expect = 0.0e+00
Identity = 747/761 (98.16%), Postives = 753/761 (98.95%), Query Frame = 0

Query: 1   MGATILPDLGAEIFIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSAAAKNGYSDYL 60
           M  TILPDLG EIFIPVCA+VGIVFSLVQWYYVSQVKLSPGRD+AHNNSA +KNGYSDYL
Sbjct: 1   MSVTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120
           IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120

Query: 121 TKPQACTYDKTKICKPALATAIFSTVSFLLGAVTSVISGFLGMKIATYANARTTLEARKG 180
           TKPQ CTYDKT+ CKPALATAIFSTVSFLLGAVTSV+SGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PALKKQLIISTVLMTFGIAIVTWLAVPSSFTIFNFGTQKEVQNWKLFLCVAVGLWAGLII 420
           PALKKQLIISTVLMTFGIAIVTWL+VPSSFTIFNFGTQK V NWKLFLCVAVGLWAGLII
Sbjct: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG 762
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG 761

BLAST of Lag0034272 vs. ExPASy TrEMBL
Match: A0A6J1KSL0 (H(+)-exporting diphosphatase OS=Cucurbita maxima OX=3661 GN=LOC111496068 PE=3 SV=1)

HSP 1 Score: 1411.0 bits (3651), Expect = 0.0e+00
Identity = 745/761 (97.90%), Postives = 753/761 (98.95%), Query Frame = 0

Query: 1   MGATILPDLGAEIFIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSAAAKNGYSDYL 60
           M  TILPDLG EIFIPVCA++GI+FSLVQWYYVSQVKLSPGRD+AHNNSA +KNGYSDYL
Sbjct: 1   MSVTILPDLGTEIFIPVCAVIGIIFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120
           IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120

Query: 121 TKPQACTYDKTKICKPALATAIFSTVSFLLGAVTSVISGFLGMKIATYANARTTLEARKG 180
           TKPQ CTYDKT+ CKPALATAIFSTVSFLLGAVTSV+SGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PALKKQLIISTVLMTFGIAIVTWLAVPSSFTIFNFGTQKEVQNWKLFLCVAVGLWAGLII 420
           PALKKQLIISTVLMTFGIAIVTWL+VPSSFTIFNFGTQK V NWKLFLCVAVGLWAGLII
Sbjct: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG 762
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG 761

BLAST of Lag0034272 vs. ExPASy TrEMBL
Match: O82680 (H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=PHP-1 PE=2 SV=1)

HSP 1 Score: 1407.9 bits (3643), Expect = 0.0e+00
Identity = 744/761 (97.77%), Postives = 752/761 (98.82%), Query Frame = 0

Query: 1   MGATILPDLGAEIFIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSAAAKNGYSDYL 60
           M  TILPDLG EIFIPVCA++GIVFSLVQWYYVSQVKLSPGRD+AHNNSA +KNGYSDYL
Sbjct: 1   MSVTILPDLGTEIFIPVCAVIGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120
           IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120

Query: 121 TKPQACTYDKTKICKPALATAIFSTVSFLLGAVTSVISGFLGMKIATYANARTTLEARKG 180
           TKPQ CTYDKT+ CKPALATAIFSTVSFLLGAVTSV+SGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASIS FGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISPFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PALKKQLIISTVLMTFGIAIVTWLAVPSSFTIFNFGTQKEVQNWKLFLCVAVGLWAGLII 420
           PALKKQLIISTVLMTFGIAIVTWL+VPSSFTIFNFGTQK V NWKLFLCVAVGLWAGLII
Sbjct: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGAS+HARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASKHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG 762
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG 761

BLAST of Lag0034272 vs. ExPASy TrEMBL
Match: A0A6J1H8E2 (H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=LOC111461438 PE=3 SV=1)

HSP 1 Score: 1404.4 bits (3634), Expect = 0.0e+00
Identity = 740/758 (97.63%), Postives = 753/758 (99.34%), Query Frame = 0

Query: 4   TILPDLGAEIFIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSAAAKNGYSDYLIEE 63
           TILPDLG +IFIPVCA+VGIVFSLVQWYYVSQVKLSP RDS HNNSAAAKNGYSDYLIEE
Sbjct: 5   TILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPSRDSTHNNSAAAKNGYSDYLIEE 64

Query: 64  EEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFSTKP 123
           EEGVNDHNVVIKCAEIQ+AISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVE FST+P
Sbjct: 65  EEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGFSTEP 124

Query: 124 QACTYDKTKICKPALATAIFSTVSFLLGAVTSVISGFLGMKIATYANARTTLEARKGVGK 183
           QACTYDKTKICKPALATAIFST+SFLLGAVTSV+SGFLGMKIATYANARTTLEARKGVGK
Sbjct: 125 QACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGK 184

Query: 184 AFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALFG 243
           AFITAFRSGAVMGFLLAANGLLVLFIAIN+FKLYYG+DWGGLFESITGYGLGGSSMALFG
Sbjct: 185 AFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSMALFG 244

Query: 244 RVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 303
           RVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE
Sbjct: 245 RVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 304

Query: 304 SSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPAL 363
           SSCAALVVASISSFGNNHELTPMLYPLIVSS+GILVCLITTLFATDFFEIKAVKEIEPAL
Sbjct: 305 SSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEIEPAL 364

Query: 364 KKQLIISTVLMTFGIAIVTWLAVPSSFTIFNFGTQKEVQNWKLFLCVAVGLWAGLIIGFV 423
           KKQLIISTVLMTFGIA+VTWLAVPS+FTIFNFGTQK VQNWKLFLCVAVGLWAGLIIGFV
Sbjct: 365 KKQLIISTVLMTFGIAVVTWLAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLIIGFV 424

Query: 424 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYG 483
           TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYG
Sbjct: 425 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYG 484

Query: 484 IAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 543
           IAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK
Sbjct: 485 IAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 544

Query: 544 GFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAA 603
           GFAIGSAALVSLALFGAFVSRAGVT+VDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAA
Sbjct: 545 GFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAA 604

Query: 604 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFG 663
           LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFG
Sbjct: 605 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFG 664

Query: 664 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVI 723
           VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVI
Sbjct: 665 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVI 724

Query: 724 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG 762
           GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HG
Sbjct: 725 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHG 762

BLAST of Lag0034272 vs. ExPASy TrEMBL
Match: A0A6J1JAU2 (H(+)-exporting diphosphatase OS=Cucurbita maxima OX=3661 GN=LOC111485119 PE=3 SV=1)

HSP 1 Score: 1403.7 bits (3632), Expect = 0.0e+00
Identity = 740/758 (97.63%), Postives = 753/758 (99.34%), Query Frame = 0

Query: 4   TILPDLGAEIFIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSAAAKNGYSDYLIEE 63
           TILPDLG +IFIPVCA+VGIVFSLVQWYYVSQVKLSP RDS HNNSAAAKNGYSDYLIEE
Sbjct: 5   TILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPSRDSTHNNSAAAKNGYSDYLIEE 64

Query: 64  EEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFSTKP 123
           EEGVNDHNVVIKCAEIQ+AISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVE FST+P
Sbjct: 65  EEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGFSTEP 124

Query: 124 QACTYDKTKICKPALATAIFSTVSFLLGAVTSVISGFLGMKIATYANARTTLEARKGVGK 183
           QACTYDKTKICKPALATAIFST+SFLLGAVTSV+SGFLGMKIATYANARTTLEARKGVGK
Sbjct: 125 QACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGK 184

Query: 184 AFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALFG 243
           AFITAFRSGAVMGFLLAANGLLVLFIAIN+FKLYYG+DWGGLFESITGYGLGGSSMALFG
Sbjct: 185 AFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSMALFG 244

Query: 244 RVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 303
           RVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE
Sbjct: 245 RVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 304

Query: 304 SSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPAL 363
           SSCAALVVASISSFGNNHELTPMLYPLIVSS+GILVCLITTLFATDFFEIKAVKEIEPAL
Sbjct: 305 SSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEIEPAL 364

Query: 364 KKQLIISTVLMTFGIAIVTWLAVPSSFTIFNFGTQKEVQNWKLFLCVAVGLWAGLIIGFV 423
           KKQLIISTVLMTFGIAIVTWLAVPS+FTIFNFGTQK VQNWKLFLCV+VGLWAGLIIGFV
Sbjct: 365 KKQLIISTVLMTFGIAIVTWLAVPSTFTIFNFGTQKVVQNWKLFLCVSVGLWAGLIIGFV 424

Query: 424 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYG 483
           TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYG
Sbjct: 425 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYG 484

Query: 484 IAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 543
           IAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK
Sbjct: 485 IAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 544

Query: 544 GFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAA 603
           GFAIGSAALVSLALFGAFVSRAGVT+VDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAA
Sbjct: 545 GFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAA 604

Query: 604 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFG 663
           LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFG
Sbjct: 605 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFG 664

Query: 664 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVI 723
           VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVI
Sbjct: 665 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVI 724

Query: 724 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG 762
           GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HG
Sbjct: 725 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHG 762

BLAST of Lag0034272 vs. TAIR 10
Match: AT1G15690.1 (Inorganic H pyrophosphatase family protein )

HSP 1 Score: 1268.4 bits (3281), Expect = 0.0e+00
Identity = 662/758 (87.34%), Postives = 710/758 (93.67%), Query Frame = 0

Query: 5   ILPDLGAEIFIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSA-AAKNGYSDYLIEE 64
           +LP+L  EI +P+CA++GI FSL QWY VS+VKL+    ++ +  A   KNGY DYLIEE
Sbjct: 6   LLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGDYLIEE 65

Query: 65  EEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFSTKP 124
           EEGVND +VV KCAEIQ+AISEGATSFLFTEYKYVG+FM+ FA +IFVFLGSVE FST  
Sbjct: 66  EEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTDN 125

Query: 125 QACTYDKTKICKPALATAIFSTVSFLLGAVTSVISGFLGMKIATYANARTTLEARKGVGK 184
           + CTYD T+ CKPALATA FST++F+LGAVTSV+SGFLGMKIATYANARTTLEARKGVGK
Sbjct: 126 KPCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVGK 185

Query: 185 AFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALFG 244
           AFI AFRSGAVMGFLLAA+GLLVL+I IN+FK+YYGDDW GLFE+ITGYGLGGSSMALFG
Sbjct: 186 AFIVAFRSGAVMGFLLAASGLLVLYITINVFKIYYGDDWEGLFEAITGYGLGGSSMALFG 245

Query: 245 RVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 304
           RVGGGIYTKAADVGADLVGK+ERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE
Sbjct: 246 RVGGGIYTKAADVGADLVGKIERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 305

Query: 305 SSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPAL 364
           +SCAALVVASISSFG NH+ T M YPL++SSMGILVCLITTLFATDFFEIK VKEIEPAL
Sbjct: 306 ASCAALVVASISSFGINHDFTAMCYPLLISSMGILVCLITTLFATDFFEIKLVKEIEPAL 365

Query: 365 KKQLIISTVLMTFGIAIVTWLAVPSSFTIFNFGTQKEVQNWKLFLCVAVGLWAGLIIGFV 424
           K QLIISTV+MT GIAIV+W+ +P+SFTIFNFGTQK V+NW+LFLCV VGLWAGLIIGFV
Sbjct: 366 KNQLIISTVIMTVGIAIVSWVGLPTSFTIFNFGTQKVVKNWQLFLCVCVGLWAGLIIGFV 425

Query: 425 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYG 484
           TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA+SIFVSF+FAAMYG
Sbjct: 426 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFSFAAMYG 485

Query: 485 IAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 544
           +AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK
Sbjct: 486 VAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 545

Query: 545 GFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAA 604
           GFAIGSAALVSLALFGAFVSRAG+  VDVLTPKV IGL+VGAMLPYWFSAMTMKSVGSAA
Sbjct: 546 GFAIGSAALVSLALFGAFVSRAGIHTVDVLTPKVIIGLLVGAMLPYWFSAMTMKSVGSAA 605

Query: 605 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFG 664
           LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPG LVMLTPLIVG  FG
Sbjct: 606 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGCLVMLTPLIVGFFFG 665

Query: 665 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVI 724
           VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAG SEHA++LGPKGS+PHKAAVI
Sbjct: 666 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGVSEHAKSLGPKGSEPHKAAVI 725

Query: 725 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHG 762
           GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HG
Sbjct: 726 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHG 763

BLAST of Lag0034272 vs. TAIR 10
Match: AT1G15690.2 (Inorganic H pyrophosphatase family protein )

HSP 1 Score: 1035.0 bits (2675), Expect = 3.3e-302
Identity = 540/628 (85.99%), Postives = 584/628 (92.99%), Query Frame = 0

Query: 5   ILPDLGAEIFIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSA-AAKNGYSDYLIEE 64
           +LP+L  EI +P+CA++GI FSL QWY VS+VKL+    ++ +  A   KNGY DYLIEE
Sbjct: 6   LLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGDYLIEE 65

Query: 65  EEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFSTKP 124
           EEGVND +VV KCAEIQ+AISEGATSFLFTEYKYVG+FM+ FA +IFVFLGSVE FST  
Sbjct: 66  EEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTDN 125

Query: 125 QACTYDKTKICKPALATAIFSTVSFLLGAVTSVISGFLGMKIATYANARTTLEARKGVGK 184
           + CTYD T+ CKPALATA FST++F+LGAVTSV+SGFLGMKIATYANARTTLEARKGVGK
Sbjct: 126 KPCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVGK 185

Query: 185 AFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALFG 244
           AFI AFRSGAVMGFLLAA+GLLVL+I IN+FK+YYGDDW GLFE+ITGYGLGGSSMALFG
Sbjct: 186 AFIVAFRSGAVMGFLLAASGLLVLYITINVFKIYYGDDWEGLFEAITGYGLGGSSMALFG 245

Query: 245 RVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 304
           RVGGGIYTKAADVGADLVGK+ERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE
Sbjct: 246 RVGGGIYTKAADVGADLVGKIERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 305

Query: 305 SSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPAL 364
           +SCAALVVASISSFG NH+ T M YPL++SSMGILVCLITTLFATDFFEIK VKEIEPAL
Sbjct: 306 ASCAALVVASISSFGINHDFTAMCYPLLISSMGILVCLITTLFATDFFEIKLVKEIEPAL 365

Query: 365 KKQLIISTVLMTFGIAIVTWLAVPSSFTIFNFGTQKEVQNWKLFLCVAVGLWAGLIIGFV 424
           K QLIISTV+MT GIAIV+W+ +P+SFTIFNFGTQK V+NW+LFLCV VGLWAGLIIGFV
Sbjct: 366 KNQLIISTVIMTVGIAIVSWVGLPTSFTIFNFGTQKVVKNWQLFLCVCVGLWAGLIIGFV 425

Query: 425 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYG 484
           TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA+SIFVSF+FAAMYG
Sbjct: 426 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFSFAAMYG 485

Query: 485 IAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 544
           +AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK
Sbjct: 486 VAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 545

Query: 545 GFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAA 604
           GFAIGSAALVSLALFGAFVSRAG+  VDVLTPKV IGL+VGAMLPYWFSAMTMKSVGSAA
Sbjct: 546 GFAIGSAALVSLALFGAFVSRAGIHTVDVLTPKVIIGLLVGAMLPYWFSAMTMKSVGSAA 605

Query: 605 LKMVEEVRRQFNTIPGLMEGTAKPDYAT 632
           LKMVEEVRRQFNTIPGLMEGTAKPDYAT
Sbjct: 606 LKMVEEVRRQFNTIPGLMEGTAKPDYAT 633

BLAST of Lag0034272 vs. TAIR 10
Match: AT1G16780.1 (Inorganic H pyrophosphatase family protein )

HSP 1 Score: 424.1 bits (1089), Expect = 2.7e-118
Identity = 289/729 (39.64%), Postives = 416/729 (57.06%), Query Frame = 0

Query: 78  EIQSAISEGATSFLFTEYKYVG--IFMVLFAVL-IFVFLGSVESFSTKPQACTYDKTKIC 137
           +I  AI +GA  FL T+Y  +    F++ F +L I++F     + + + +A    +T   
Sbjct: 150 QISDAIRDGAEGFLRTQYGTISKMAFLLAFVILCIYLF----RNLTPQQEASGLGRT--- 209

Query: 138 KPALATAIFSTVSFLLGAVTSVISGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAV 197
                +A  +  +FLLGA+ S I+G++GM ++  AN R +  AR+   +A   A R+G  
Sbjct: 210 ----MSAYITVAAFLLGALCSGIAGYVGMWVSVRANVRVSSAARRSAREALQIAVRAGGF 269

Query: 198 MGFLLAANGLLVLFIAINLFKLYYGDDWGGLFE------SITGYGLGGSSMALFGRVGGG 257
              ++    ++ + I  + F ++   D  G  +       + GYG G S +ALF ++GGG
Sbjct: 270 SALVVVGMAVIGIAILYSTFYVWLDVDSPGSMKVTDLPLLLVGYGFGASFVALFAQLGGG 329

Query: 258 IYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAA 317
           IYTK ADVGADLVGKVE  IPEDDPRNPAVIAD VGDNVGD A  G+DLF S A    +A
Sbjct: 330 IYTKGADVGADLVGKVEHGIPEDDPRNPAVIADLVGDNVGDCAARGADLFESIAAEIISA 389

Query: 318 LVVASISSFGNNHELTP--MLYPLIVSSMGILVCL--ITTLFATDFFEIKA-VKEIEPAL 377
           +++    +     E     +L+PL+V S  +++    I ++  T    +K+ V++    L
Sbjct: 390 MILGGTMAQKCKIEDPSGFILFPLVVHSFDLVISSIGILSIKGTRNASVKSPVEDPMVVL 449

Query: 378 KK--QLIISTVLMTFGIAIVTWLAVPSSFTIFNFGTQKEVQNW-KLFLCVAVGLWAGLII 437
           +K   L I   ++TFG A   WL            T++    W   F+C  VG+    + 
Sbjct: 450 QKGYSLTIILAVLTFG-ASTRWLLY----------TEQAPSAWLNFFMCGLVGIITAYVF 509

Query: 438 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF-- 497
            +++ YYT   Y PV+ +A +  TG  TN+I G++LG +S  +P+  I+V+I  +F    
Sbjct: 510 VWISRYYTDYKYEPVRTLALASSTGHGTNIIAGVSLGLESTALPVLVISVAIISAFWLGN 569

Query: 498 ------------AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRE 557
                         ++G AVA +GMLST A  L +D +GPI+DNAGGI EM+     +RE
Sbjct: 570 TSGLIDEKGNPTGGLFGTAVATMGMLSTAAYVLTMDMFGPIADNAGGIVEMSQQPESVRE 629

Query: 558 RTDALDAAGNTTAAIGKGFAIGSAALVSLALFGAFVSRAGVTA------VDVLTPKVFIG 617
            TD LDA GNTT A  KGFAIGSAAL S  LF A++      A      VD+  P+VFIG
Sbjct: 630 ITDVLDAVGNTTKATTKGFAIGSAALASFLLFSAYMDEVSAFANVSFKEVDIAIPEVFIG 689

Query: 618 LIVGAMLPYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDAS 677
            ++GAML + FSA    +VG  A ++V EVRRQF   PG+M+   KPDY  CV I   ++
Sbjct: 690 GLLGAMLIFLFSAWACAAVGRTAQEVVNEVRRQFIERPGIMDYKEKPDYGRCVAIVASSA 749

Query: 678 IKEMIPPGALVMLTPLIVGILF------------GVETLSGVLAGSLVSGVQIAISASNT 737
           ++EMI PGAL +++P+ VG +F            G + ++ +L  + V G+ +A+  +  
Sbjct: 750 LREMIKPGALAIISPIAVGFVFRILGYYTGQPLLGAKVVAAMLMFATVCGILMALFLNTA 809

Query: 738 GGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPLKDTSGPSLNILIKLMAV 758
           GGAWDNAKKYIE GA      LG KGSD HKAAV GDT+GDP KDT+GPS+++LIK++A 
Sbjct: 810 GGAWDNAKKYIETGA------LGGKGSDSHKAAVTGDTVGDPFKDTAGPSIHVLIKMLAT 850

BLAST of Lag0034272 vs. TAIR 10
Match: AT1G78920.1 (vacuolar H+-pyrophosphatase 2 )

HSP 1 Score: 421.8 bits (1083), Expect = 1.3e-117
Identity = 290/730 (39.73%), Postives = 418/730 (57.26%), Query Frame = 0

Query: 78  EIQSAISEGATSFLFTEYKYVGIFMVLFA-VLIFVFLGSVESFSTKPQACTYDKTKICKP 137
           EI  AI +GA  F  T+Y  +    +L A V++ ++L    S + + +A    +      
Sbjct: 101 EISDAIRDGAEGFFRTQYSTISKMAILLAFVILCIYL--FRSLTPQQEAAGLGRA----- 160

Query: 138 ALATAIFSTVSFLLGAVTSVISGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAVMG 197
              +A  +  +FLLGA+ S I+G++GM ++  AN R +  AR+   +A   A R+G    
Sbjct: 161 --MSAYITVAAFLLGALCSGIAGYVGMWVSVRANVRVSSAARRSAREALQIAVRAGGFSA 220

Query: 198 FLLAANGLLVLFIAINLFKLYYGDDWGGLFE------SITGYGLGGSSMALFGRVGGGIY 257
            ++    ++ + I  + F ++ G    G          + GYG G S +ALF ++GGGIY
Sbjct: 221 LVVVGMAVIGIAILYSTFYVWLGVGSPGSMNVTDLPLLLVGYGFGASFVALFAQLGGGIY 280

Query: 258 TKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALV 317
           TK ADVGADLVGKVE+ IPEDDPRNPAVIAD VGDNVGD A  G+DLF S A    +A++
Sbjct: 281 TKGADVGADLVGKVEQGIPEDDPRNPAVIADLVGDNVGDCAARGADLFESIAAEIISAMI 340

Query: 318 VASISSFGNNHELTP--MLYPLIVSSMGILVCL--ITTLFATDFFEIKA-VKEIEPALKK 377
           +    +     E     +L+PL+V S  +++    I ++  T    +K+ V++    L+K
Sbjct: 341 LGGTMAKKCKIEDPSGFILFPLVVHSFDLIISSIGILSIKGTRDASVKSPVEDPMAVLQK 400

Query: 378 --QLIISTVLMTFGIAIVTWL----AVPSSFTIFNFGTQKEVQNWKLFLCVAVGLWAGLI 437
              L I   ++TFG A   WL      PS++  FNF            LC  VG+    I
Sbjct: 401 GYSLTIILAVITFG-ASTRWLLYTEQAPSAW--FNFA-----------LCGLVGIITAYI 460

Query: 438 IGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF- 497
             ++++YYT   + PV+ +A +  TG  TN+I G++LG +S  +P+  I+V+I  ++   
Sbjct: 461 FVWISKYYTDYKHEPVRTLALASSTGHGTNIIAGVSLGLESTALPVLTISVAIISAYWLG 520

Query: 498 -------------AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIR 557
                          ++G AVA +GMLST A  L +D +GPI+DNAGGI EM+     +R
Sbjct: 521 NTSGLVDENGIPTGGLFGTAVATMGMLSTAAYVLTMDMFGPIADNAGGIVEMSQQPESVR 580

Query: 558 ERTDALDAAGNTTAAIGKGFAIGSAALVSLALFGAFVSRAGVTA------VDVLTPKVFI 617
           E TD LDA GNTT A  KGFAIGSAAL S  LF A++      A      VD+  P+VF+
Sbjct: 581 EITDLLDAVGNTTKATTKGFAIGSAALASFLLFSAYMDEVSAFANVSFKEVDIAIPEVFV 640

Query: 618 GLIVGAMLPYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDA 677
           G ++GAML + FSA    +VG  A ++V EVRRQF   PG+ME   KPDY+ CV I   A
Sbjct: 641 GGLLGAMLIFLFSAWACAAVGRTAQEVVNEVRRQFIERPGIMEYKEKPDYSRCVAIVASA 700

Query: 678 SIKEMIPPGALVMLTPLIVGILF------------GVETLSGVLAGSLVSGVQIAISASN 737
           +++EMI PGAL + +P++VG++F            G + ++ +L  + V G+ +A+  + 
Sbjct: 701 ALREMIKPGALAIASPIVVGLVFRILGYYTGQPLLGAKVVASMLMFATVCGILMALFLNT 760

Query: 738 TGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPLKDTSGPSLNILIKLMA 758
            GGAWDNAKKYIE GA      LG KGS+ HKAAV GDT+GDP KDT+GPS+++LIK++A
Sbjct: 761 AGGAWDNAKKYIETGA------LGGKGSEAHKAAVTGDTVGDPFKDTAGPSIHVLIKMLA 801

BLAST of Lag0034272 vs. TAIR 10
Match: AT1G78920.2 (vacuolar H+-pyrophosphatase 2 )

HSP 1 Score: 421.8 bits (1083), Expect = 1.3e-117
Identity = 290/730 (39.73%), Postives = 418/730 (57.26%), Query Frame = 0

Query: 78  EIQSAISEGATSFLFTEYKYVGIFMVLFA-VLIFVFLGSVESFSTKPQACTYDKTKICKP 137
           EI  AI +GA  F  T+Y  +    +L A V++ ++L    S + + +A    +      
Sbjct: 101 EISDAIRDGAEGFFRTQYSTISKMAILLAFVILCIYL--FRSLTPQQEAAGLGRA----- 160

Query: 138 ALATAIFSTVSFLLGAVTSVISGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAVMG 197
              +A  +  +FLLGA+ S I+G++GM ++  AN R +  AR+   +A   A R+G    
Sbjct: 161 --MSAYITVAAFLLGALCSGIAGYVGMWVSVRANVRVSSAARRSAREALQIAVRAGGFSA 220

Query: 198 FLLAANGLLVLFIAINLFKLYYGDDWGGLFE------SITGYGLGGSSMALFGRVGGGIY 257
            ++    ++ + I  + F ++ G    G          + GYG G S +ALF ++GGGIY
Sbjct: 221 LVVVGMAVIGIAILYSTFYVWLGVGSPGSMNVTDLPLLLVGYGFGASFVALFAQLGGGIY 280

Query: 258 TKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALV 317
           TK ADVGADLVGKVE+ IPEDDPRNPAVIAD VGDNVGD A  G+DLF S A    +A++
Sbjct: 281 TKGADVGADLVGKVEQGIPEDDPRNPAVIADLVGDNVGDCAARGADLFESIAAEIISAMI 340

Query: 318 VASISSFGNNHELTP--MLYPLIVSSMGILVCL--ITTLFATDFFEIKA-VKEIEPALKK 377
           +    +     E     +L+PL+V S  +++    I ++  T    +K+ V++    L+K
Sbjct: 341 LGGTMAKKCKIEDPSGFILFPLVVHSFDLIISSIGILSIKGTRDASVKSPVEDPMAVLQK 400

Query: 378 --QLIISTVLMTFGIAIVTWL----AVPSSFTIFNFGTQKEVQNWKLFLCVAVGLWAGLI 437
              L I   ++TFG A   WL      PS++  FNF            LC  VG+    I
Sbjct: 401 GYSLTIILAVITFG-ASTRWLLYTEQAPSAW--FNFA-----------LCGLVGIITAYI 460

Query: 438 IGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF- 497
             ++++YYT   + PV+ +A +  TG  TN+I G++LG +S  +P+  I+V+I  ++   
Sbjct: 461 FVWISKYYTDYKHEPVRTLALASSTGHGTNIIAGVSLGLESTALPVLTISVAIISAYWLG 520

Query: 498 -------------AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIR 557
                          ++G AVA +GMLST A  L +D +GPI+DNAGGI EM+     +R
Sbjct: 521 NTSGLVDENGIPTGGLFGTAVATMGMLSTAAYVLTMDMFGPIADNAGGIVEMSQQPESVR 580

Query: 558 ERTDALDAAGNTTAAIGKGFAIGSAALVSLALFGAFVSRAGVTA------VDVLTPKVFI 617
           E TD LDA GNTT A  KGFAIGSAAL S  LF A++      A      VD+  P+VF+
Sbjct: 581 EITDLLDAVGNTTKATTKGFAIGSAALASFLLFSAYMDEVSAFANVSFKEVDIAIPEVFV 640

Query: 618 GLIVGAMLPYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDA 677
           G ++GAML + FSA    +VG  A ++V EVRRQF   PG+ME   KPDY+ CV I   A
Sbjct: 641 GGLLGAMLIFLFSAWACAAVGRTAQEVVNEVRRQFIERPGIMEYKEKPDYSRCVAIVASA 700

Query: 678 SIKEMIPPGALVMLTPLIVGILF------------GVETLSGVLAGSLVSGVQIAISASN 737
           +++EMI PGAL + +P++VG++F            G + ++ +L  + V G+ +A+  + 
Sbjct: 701 ALREMIKPGALAIASPIVVGLVFRILGYYTGQPLLGAKVVASMLMFATVCGILMALFLNT 760

Query: 738 TGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPLKDTSGPSLNILIKLMA 758
            GGAWDNAKKYIE GA      LG KGS+ HKAAV GDT+GDP KDT+GPS+++LIK++A
Sbjct: 761 AGGAWDNAKKYIETGA------LGGKGSEAHKAAVTGDTVGDPFKDTAGPSIHVLIKMLA 801

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022929683.10.0e+0098.16pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita moschata] ... [more]
XP_023002108.10.0e+0097.90pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita maxima][more]
XP_023529940.10.0e+0097.90pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita pepo subsp... [more]
BAA33149.10.0e+0097.77proton-translocating inorganic pyrophosphatase [Cucurbita moschata][more]
XP_023515840.10.0e+0097.76pyrophosphate-energized vacuolar membrane proton pump [Cucurbita pepo subsp. pep... [more]
Match NameE-valueIdentityDescription
P216160.0e+0091.98Pyrophosphate-energized vacuolar membrane proton pump OS=Vigna radiata var. radi... [more]
P314140.0e+0087.34Pyrophosphate-energized vacuolar membrane proton pump 1 OS=Arabidopsis thaliana ... [more]
Q065720.0e+0087.45Pyrophosphate-energized vacuolar membrane proton pump OS=Hordeum vulgare OX=4513... [more]
Q72Q298.2e-19756.81Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira inter... [more]
Q8F6418.2e-19756.81Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira inter... [more]
Match NameE-valueIdentityDescription
A0A6J1ESX20.0e+0098.16H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=LOC111436191 PE=3 ... [more]
A0A6J1KSL00.0e+0097.90H(+)-exporting diphosphatase OS=Cucurbita maxima OX=3661 GN=LOC111496068 PE=3 SV... [more]
O826800.0e+0097.77H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=PHP-1 PE=2 SV=1[more]
A0A6J1H8E20.0e+0097.63H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=LOC111461438 PE=3 ... [more]
A0A6J1JAU20.0e+0097.63H(+)-exporting diphosphatase OS=Cucurbita maxima OX=3661 GN=LOC111485119 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT1G15690.10.0e+0087.34Inorganic H pyrophosphatase family protein [more]
AT1G15690.23.3e-30285.99Inorganic H pyrophosphatase family protein [more]
AT1G16780.12.7e-11839.64Inorganic H pyrophosphatase family protein [more]
AT1G78920.11.3e-11739.73vacuolar H+-pyrophosphatase 2 [more]
AT1G78920.21.3e-11739.73vacuolar H+-pyrophosphatase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004131Pyrophosphate-energised proton pumpTIGRFAMTIGR01104TIGR01104coord: 11..761
e-value: 0.0
score: 1228.7
IPR004131Pyrophosphate-energised proton pumpPFAMPF03030H_PPasecoord: 42..753
e-value: 5.9E-257
score: 854.5
IPR004131Pyrophosphate-energised proton pumpPIRSFPIRSF001265H+-PPtasecoord: 11..766
e-value: 3.4E-283
score: 938.6
IPR004131Pyrophosphate-energised proton pumpPANTHERPTHR31998K(+)-INSENSITIVE PYROPHOSPHATE-ENERGIZED PROTON PUMPcoord: 1..762
IPR004131Pyrophosphate-energised proton pumpHAMAPMF_01129PPase_energized_pumpcoord: 9..758
score: 23.106735
NoneNo IPR availablePANTHERPTHR31998:SF38PYROPHOSPHATE-ENERGIZED VACUOLAR MEMBRANE PROTON PUMPcoord: 1..762

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0034272.1Lag0034272.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902600 proton transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0004427 inorganic diphosphatase activity
molecular_function GO:0009678 pyrophosphate hydrolysis-driven proton transmembrane transporter activity