Lag0034267 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0034267
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	chr3: 5849672 .. 5851138 (+)
RNA-Seq Expression	Lag0034267
Synteny	Lag0034267

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGGAATTAACCCGCCCGGCGCCGTCCAAGGCGTGGCTTCTAAAGAGAAAGTGCCGGAGAGATACATCCATAAAGAAAGCGATCGAGGAGTTCTAGATGCACCTTTAATGGACGTTCCTGTAATTGACATCGATCTCCTCTCGTCTTCATCCAATTCCGGACCGGAACTCCAGAAATTCCGACAAGGACTTGATTCATGGGGCTGCTTTCAGGTTAGATTCCAGTGAACTTAAAACCCTAATTTAAATCGGATTGCATGGCGACCCTTTCTGGTTAGATTTCAGTGGACTCTGCATCTCGATTCCAGAAAAACGTTTGGACATCGTAAATTGCTTGAAATCGGACTCAAAAAACGTCGGGATTTTGTTATCCTAATGTTTTGAGAACATGATCGAAAGTTTTCTGGTGTTGTTTCAGGTGATAAATCATGGAATGTCCTCTGAATTTCTGGAAGAAGTTCGGCAATTAACGAAACAGTTCTTTGCTCTTCCAATGGAAGAAAAATTGAAATACTCGAGAGAAGAAGATGACACTGAAGGATATGGCAACGCAACGATTCTCTTCAACGACCAAATTCTGGATTGGACTGATCGTTTGTTTATCAATGTTTATCCAGAAGAGAGCCGCCGTTTGAAGTACTGGCCAACAACTCCTGAAAGATTCAGGTAATTTTTTATTTGGTTTTTATTATGAGTAATGTACAAGAATAATCTGTACAAACAGACAGTTGGTTTCCAAACATGTTTTGGATCGAAAATGGATAATGTGTTGTGATGAACATTGCAGGGAAGTTGCTGATGAGTACACTGAAAATGTGAGGCTGCTAAGTGAGAAAATCTTCAAGGCCATGGCGAAATCATTGGATTTGGAAGATGAGAATAGCTTTGTCAACCAGTTTGTCGAGGGATTTAAACTGGACGCATATTTCAACTTATACCCTCAATGTCGAAATCCCGGTCTCGTTCTCGGTCTCAAGCCGCACTCCGATGCATCATCAATCACCATTCTGTTGCAGGACAAGGAAGTAGAAGGTCTTCAGTTCTTGAAAGACAATGAGTGGTTCAATGCTCCAACTCTTCCTGATGCTCTTCTCGTCAATGTTGGGGATCAAGTAGAGGTAAACACACTACTCTTTACATTTACAAAAACTAGTTATAACTAAATCACTTTAGCTAGCTAAAAGCACTTGTTCTAAAATCACTTGGGTTGACTTGAAATTTGTGTAGATAACAAGTAATGGGATATTCAAGAGTCCAATTCATAGAGTATTGACAAACACAGAGAGGGAGAGGATTTCATTGGCAGTGTTTTTCCTCCCAGATTCAGAGAAAGAAATTGAACCACTTGAGGAGCTCATCACTGAAACTCGGCCAAGGTTGTACAAGACTGTCAAGAACTTTGCTGCCGATTACTATCAGTACTACCAGAAAGGCCAAAGGCCAATCGAGGCTGCAAGAATCTAG

mRNA sequence

ATGGCTGGAATTAACCCGCCCGGCGCCGTCCAAGGCGTGGCTTCTAAAGAGAAAGTGCCGGAGAGATACATCCATAAAGAAAGCGATCGAGGAGTTCTAGATGCACCTTTAATGGACGTTCCTGTAATTGACATCGATCTCCTCTCGTCTTCATCCAATTCCGGACCGGAACTCCAGAAATTCCGACAAGGACTTGATTCATGGGGCTGCTTTCAGGTGATAAATCATGGAATGTCCTCTGAATTTCTGGAAGAAGTTCGGCAATTAACGAAACAGTTCTTTGCTCTTCCAATGGAAGAAAAATTGAAATACTCGAGAGAAGAAGATGACACTGAAGGATATGGCAACGCAACGATTCTCTTCAACGACCAAATTCTGGATTGGACTGATCGTTTGTTTATCAATGTTTATCCAGAAGAGAGCCGCCGTTTGAAGTACTGGCCAACAACTCCTGAAAGATTCAGGGAAGTTGCTGATGAGTACACTGAAAATGTGAGGCTGCTAAGTGAGAAAATCTTCAAGGCCATGGCGAAATCATTGGATTTGGAAGATGAGAATAGCTTTGTCAACCAGTTTGTCGAGGGATTTAAACTGGACGCATATTTCAACTTATACCCTCAATGTCGAAATCCCGGTCTCGTTCTCGGTCTCAAGCCGCACTCCGATGCATCATCAATCACCATTCTGTTGCAGGACAAGGAAGTAGAAGGTCTTCAGTTCTTGAAAGACAATGAGTGGTTCAATGCTCCAACTCTTCCTGATGCTCTTCTCGTCAATGTTGGGGATCAAGTAGAGATAACAAGTAATGGGATATTCAAGAGTCCAATTCATAGAGTATTGACAAACACAGAGAGGGAGAGGATTTCATTGGCAGTGTTTTTCCTCCCAGATTCAGAGAAAGAAATTGAACCACTTGAGGAGCTCATCACTGAAACTCGGCCAAGGTTGTACAAGACTGTCAAGAACTTTGCTGCCGATTACTATCAGTACTACCAGAAAGGCCAAAGGCCAATCGAGGCTGCAAGAATCTAG

Coding sequence (CDS)

ATGGCTGGAATTAACCCGCCCGGCGCCGTCCAAGGCGTGGCTTCTAAAGAGAAAGTGCCGGAGAGATACATCCATAAAGAAAGCGATCGAGGAGTTCTAGATGCACCTTTAATGGACGTTCCTGTAATTGACATCGATCTCCTCTCGTCTTCATCCAATTCCGGACCGGAACTCCAGAAATTCCGACAAGGACTTGATTCATGGGGCTGCTTTCAGGTGATAAATCATGGAATGTCCTCTGAATTTCTGGAAGAAGTTCGGCAATTAACGAAACAGTTCTTTGCTCTTCCAATGGAAGAAAAATTGAAATACTCGAGAGAAGAAGATGACACTGAAGGATATGGCAACGCAACGATTCTCTTCAACGACCAAATTCTGGATTGGACTGATCGTTTGTTTATCAATGTTTATCCAGAAGAGAGCCGCCGTTTGAAGTACTGGCCAACAACTCCTGAAAGATTCAGGGAAGTTGCTGATGAGTACACTGAAAATGTGAGGCTGCTAAGTGAGAAAATCTTCAAGGCCATGGCGAAATCATTGGATTTGGAAGATGAGAATAGCTTTGTCAACCAGTTTGTCGAGGGATTTAAACTGGACGCATATTTCAACTTATACCCTCAATGTCGAAATCCCGGTCTCGTTCTCGGTCTCAAGCCGCACTCCGATGCATCATCAATCACCATTCTGTTGCAGGACAAGGAAGTAGAAGGTCTTCAGTTCTTGAAAGACAATGAGTGGTTCAATGCTCCAACTCTTCCTGATGCTCTTCTCGTCAATGTTGGGGATCAAGTAGAGATAACAAGTAATGGGATATTCAAGAGTCCAATTCATAGAGTATTGACAAACACAGAGAGGGAGAGGATTTCATTGGCAGTGTTTTTCCTCCCAGATTCAGAGAAAGAAATTGAACCACTTGAGGAGCTCATCACTGAAACTCGGCCAAGGTTGTACAAGACTGTCAAGAACTTTGCTGCCGATTACTATCAGTACTACCAGAAAGGCCAAAGGCCAATCGAGGCTGCAAGAATCTAG

Protein sequence

MAGINPPGAVQGVASKEKVPERYIHKESDRGVLDAPLMDVPVIDIDLLSSSSNSGPELQKFRQGLDSWGCFQVINHGMSSEFLEEVRQLTKQFFALPMEEKLKYSREEDDTEGYGNATILFNDQILDWTDRLFINVYPEESRRLKYWPTTPERFREVADEYTENVRLLSEKIFKAMAKSLDLEDENSFVNQFVEGFKLDAYFNLYPQCRNPGLVLGLKPHSDASSITILLQDKEVEGLQFLKDNEWFNAPTLPDALLVNVGDQVEITSNGIFKSPIHRVLTNTERERISLAVFFLPDSEKEIEPLEELITETRPRLYKTVKNFAADYYQYYQKGQRPIEAARI

Homology

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BLAST of Lag0034267 vs. NCBI nr
Match: KAA0050082.1 (protein SRG1-like [Cucumis melo var. makuwa])

HSP 1 Score: 544.3 bits (1401), Expect = 7.7e-151
Identity = 262/343 (76.38%), Postives = 297/343 (86.59%), Query Frame = 0


Query: 1   MAGINPPGAVQGVASKEKVPERYIHKESDRGVLDAPLMDVPVIDIDLLSSSSNSGPELQK 60
           MA  NP G VQ VASK +VPERYIHKESDRG  +APLM  PVIDI LLSSSS SGPEL+K
Sbjct: 20  MASTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 79

Query: 61  FRQGLDSWGCFQVINHGMSSEFLEEVRQLTKQFFALPMEEKLKYSREEDDTEGYGNATIL 120
            R GL SWGCFQ INHGM+SE+L+EVRQLTKQFF L MEEKLKY +EE + EGYGN  IL
Sbjct: 80  LRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMIL 139

Query: 121 FNDQILDWTDRLFINVYPEESRRLKYWPTTPERFREVADEYTENVRLLSEKIFKAMAKSL 180
            N QILDWTDRL++ VYP +SRR KYWPT P+RFREV  EYT NV+L+SEKI KAMA+SL
Sbjct: 140 SNQQILDWTDRLYLTVYPHQSRRFKYWPTNPQRFREVVGEYTTNVKLISEKILKAMARSL 199

Query: 181 DLEDENSFVNQFVEGFKLDAYFNLYPQCRNPGLVLGLKPHSDASSITILLQDKEVEGLQF 240
           DL DE+SF+NQ+ E  KLDA FN YP+CRNP LVLG+KPH+D S+ITILLQDKEVEGLQF
Sbjct: 200 DL-DESSFLNQYGEQVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQF 259

Query: 241 LKDNEWFNAPTLPDALLVNVGDQVEITSNGIFKSPIHRVLTNTERERISLAVFFLPDSEK 300
           +KDNEW+NAP +PDALLVNVGDQVEITSNGIFKSP+HRVLTN+ERERISLAVF+LPDSEK
Sbjct: 260 MKDNEWYNAPIVPDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEK 319

Query: 301 EIEPLEELITETRPRLYKTVKNFAADYYQYYQKGQRPIEAARI 344
           EIEPLEELI E++PRLYK+VKNF   Y++YYQ+GQRP+EAARI
Sbjct: 320 EIEPLEELINESQPRLYKSVKNFVGLYFEYYQQGQRPMEAARI 361

[Close]

BLAST of Lag0034267 vs. NCBI nr
Match: XP_011655688.2 (probable 2-oxoglutarate-dependent dioxygenase ANS [Cucumis sativus] >KAE8653027.1 hypothetical protein Csa_019640 [Cucumis sativus])

HSP 1 Score: 541.2 bits (1393), Expect = 6.5e-150
Identity = 262/343 (76.38%), Postives = 298/343 (86.88%), Query Frame = 0


Query: 1   MAGINPPGAVQGVASKEKVPERYIHKESDRGVLDAPLMDVPVIDIDLLSSSSNSGPELQK 60
           MAG NP G VQ VASK +VPERYIHKESDRG  +APLM  PVIDI LLSSSS SGPEL+K
Sbjct: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 60

Query: 61  FRQGLDSWGCFQVINHGMSSEFLEEVRQLTKQFFALPMEEKLKYSREEDDTEGYGNATIL 120
            R GL SWGCFQ INHGM+SE+L+EVR+LTKQFF L MEEKLKY +EE + EGYGN  IL
Sbjct: 61  LRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGLSMEEKLKYLKEELEMEGYGNDMIL 120

Query: 121 FNDQILDWTDRLFINVYPEESRRLKYWPTTPERFREVADEYTENVRLLSEKIFKAMAKSL 180
            N QILDWTDRL++ VYP +SRR KYWPT  +RFREV DEYT NV+L+SEKIFKAMA+SL
Sbjct: 121 SNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFREVIDEYTTNVKLISEKIFKAMARSL 180

Query: 181 DLEDENSFVNQFVEGFKLDAYFNLYPQCRNPGLVLGLKPHSDASSITILLQDKEVEGLQF 240
           DL DE+SF+ Q+ E  KLDA FN YP+CRNP LVLG+KPH+D S+ITILLQDKEVEGLQF
Sbjct: 181 DL-DESSFLKQYGEQIKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQF 240

Query: 241 LKDNEWFNAPTLPDALLVNVGDQVEITSNGIFKSPIHRVLTNTERERISLAVFFLPDSEK 300
           +KDNEWFNAP +PDALLVNVGDQVEITSNGIFKSP+HRVLTN+ERERISLAVF+LPD+EK
Sbjct: 241 MKDNEWFNAPIVPDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDAEK 300

Query: 301 EIEPLEELITETRPRLYKTVKNFAADYYQYYQKGQRPIEAARI 344
           EIEPLEELI+ET+PRLYK+VKNF    ++YYQ+GQRP+EAARI
Sbjct: 301 EIEPLEELISETQPRLYKSVKNFVGLSFEYYQQGQRPMEAARI 342

[Close]

BLAST of Lag0034267 vs. NCBI nr
Match: XP_023516521.1 (protein SRG1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 540.0 bits (1390), Expect = 1.4e-149
Identity = 263/343 (76.68%), Postives = 294/343 (85.71%), Query Frame = 0


Query: 1   MAGINPPGAVQGVASKEKVPERYIHKESDRGVLDAPLMDVPVIDIDLLSSSSNSGPELQK 60
           MAG NP G VQGVASK +VPERYIHKESDRG  DAPLM  PVID+ LLSSSS SGPEL+K
Sbjct: 1   MAGTNPSGTVQGVASKGEVPERYIHKESDRGARDAPLMAAPVIDMALLSSSSKSGPELEK 60

Query: 61  FRQGLDSWGCFQVINHGMSSEFLEEVRQLTKQFFALPMEEKLKYSREEDDTEGYGNATIL 120
            R GL SWGCFQV+NHGMS+EFL+EVR+L KQFF LPMEEK KYSREE++ EGYGN  IL
Sbjct: 61  LRHGLQSWGCFQVVNHGMSAEFLDEVRRLAKQFFDLPMEEKSKYSREENEIEGYGNDMIL 120

Query: 121 FNDQILDWTDRLFINVYPEESRRLKYWPTTPERFREVADEYTENVRLLSEKIFKAMAKSL 180
            N QILDWTDRL++ VYP+ES R KYWPT PERFREV  EYT NV+LLSEKI KAMA SL
Sbjct: 121 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMAISL 180

Query: 181 DLEDENSFVNQFVEGFKLDAYFNLYPQCRNPGLVLGLKPHSDASSITILLQDKEVEGLQF 240
           DL +E+SF+ Q+ E  KLDA FN YP+CRNP LVLG+KPH+D S+ITILLQD+EVEGLQF
Sbjct: 181 DL-NEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQF 240

Query: 241 LKDNEWFNAPTLPDALLVNVGDQVEITSNGIFKSPIHRVLTNTERERISLAVFFLPDSEK 300
           L  NEWFNAP +P ALLVNVGDQ EITSNGIFKSP+HRVLTN+ERERISLAVF+LPD +K
Sbjct: 241 LNGNEWFNAPIVPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDWKK 300

Query: 301 EIEPLEELITETRPRLYKTVKNFAADYYQYYQKGQRPIEAARI 344
           EIEPLE+LI ETRPRLYKTVKNF   Y+QYYQ+GQRP+EAARI
Sbjct: 301 EIEPLEKLIDETRPRLYKTVKNFVGLYFQYYQQGQRPMEAARI 342

[Close]

BLAST of Lag0034267 vs. NCBI nr
Match: TYK10346.1 (protein SRG1-like [Cucumis melo var. makuwa])

HSP 1 Score: 537.7 bits (1384), Expect = 7.2e-149
Identity = 259/343 (75.51%), Postives = 295/343 (86.01%), Query Frame = 0


Query: 1   MAGINPPGAVQGVASKEKVPERYIHKESDRGVLDAPLMDVPVIDIDLLSSSSNSGPELQK 60
           MA  NP G VQ VASK +VPERYIHKESDRG  +APLM  PVIDI LLSSSS SGPEL+K
Sbjct: 20  MASTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 79

Query: 61  FRQGLDSWGCFQVINHGMSSEFLEEVRQLTKQFFALPMEEKLKYSREEDDTEGYGNATIL 120
            R GL SWGCFQ INHGM+SE+L+EVRQLTKQFF L MEEKLKY +EE + EGYGN  IL
Sbjct: 80  LRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMIL 139

Query: 121 FNDQILDWTDRLFINVYPEESRRLKYWPTTPERFREVADEYTENVRLLSEKIFKAMAKSL 180
            N QILDWTDRL++ VYP +SRR KYWP  P+RFREV  EYT NV+L+SEKI KAMA+SL
Sbjct: 140 SNQQILDWTDRLYLTVYPHQSRRFKYWPINPQRFREVVGEYTTNVKLISEKILKAMARSL 199

Query: 181 DLEDENSFVNQFVEGFKLDAYFNLYPQCRNPGLVLGLKPHSDASSITILLQDKEVEGLQF 240
           DL DE+SF+NQ+ E  KLDA FN YP+CRNP LVLG+KPH+D S+ITILLQDKEVEGLQF
Sbjct: 200 DL-DESSFLNQYGEQVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQF 259

Query: 241 LKDNEWFNAPTLPDALLVNVGDQVEITSNGIFKSPIHRVLTNTERERISLAVFFLPDSEK 300
           +KDNEW+NAP + DALLVNVGDQVEITSNGIFKSP+HRVLTN+ERERISLAVF+LPDSEK
Sbjct: 260 MKDNEWYNAPIVTDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEK 319

Query: 301 EIEPLEELITETRPRLYKTVKNFAADYYQYYQKGQRPIEAARI 344
           EIEPLEELI E++PRLYK+VKNF   Y++YYQ+G+RP+EAARI
Sbjct: 320 EIEPLEELINESQPRLYKSVKNFVGLYFEYYQQGERPMEAARI 361

[Close]

BLAST of Lag0034267 vs. NCBI nr
Match: XP_022960723.1 (protein SRG1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 537.7 bits (1384), Expect = 7.2e-149
Identity = 261/343 (76.09%), Postives = 292/343 (85.13%), Query Frame = 0


Query: 1   MAGINPPGAVQGVASKEKVPERYIHKESDRGVLDAPLMDVPVIDIDLLSSSSNSGPELQK 60
           MAG NP G VQ VASK +VPERYIHKESDRG  DAPLM  PVID+ LLSSSS SGPEL+K
Sbjct: 20  MAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAPVIDMALLSSSSESGPELEK 79

Query: 61  FRQGLDSWGCFQVINHGMSSEFLEEVRQLTKQFFALPMEEKLKYSREEDDTEGYGNATIL 120
            R GL SWGCFQV+NHGMS+EFL+E+R+L KQFF LPMEEK KYSREED+ EGYGN  IL
Sbjct: 80  LRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEIEGYGNDMIL 139

Query: 121 FNDQILDWTDRLFINVYPEESRRLKYWPTTPERFREVADEYTENVRLLSEKIFKAMAKSL 180
            N QILDWTDRL++ VYP+ES R KYWPT PERFR V  EYT NV+LLSEKI KAMA SL
Sbjct: 140 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEYTANVKLLSEKILKAMAISL 199

Query: 181 DLEDENSFVNQFVEGFKLDAYFNLYPQCRNPGLVLGLKPHSDASSITILLQDKEVEGLQF 240
           DL +E+SF+ Q+ E  KLDA FN YP+CRNP LVLG+KPH+D S+ITILLQD+EVEGLQF
Sbjct: 200 DL-NEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQF 259

Query: 241 LKDNEWFNAPTLPDALLVNVGDQVEITSNGIFKSPIHRVLTNTERERISLAVFFLPDSEK 300
           L  NEWFNAP +P ALL+NVGDQ EITSNGIFKSP+HRVLTN+ERERISLAVF+LPD EK
Sbjct: 260 LNGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDWEK 319

Query: 301 EIEPLEELITETRPRLYKTVKNFAADYYQYYQKGQRPIEAARI 344
           EIEPLE+LI ETRPRLYKTVKNF   Y+QYYQ+GQRP+EAARI
Sbjct: 320 EIEPLEKLIDETRPRLYKTVKNFVGLYFQYYQQGQRPMEAARI 361

[Close]

BLAST of Lag0034267 vs. ExPASy Swiss-Prot
Match: O80449 (Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 1.8e-62
Identity = 125/307 (40.72%), Postives = 183/307 (59.61%), Query Frame = 0


Query: 19  VPERYIHKESDRGVLDAPLMDVPVIDIDLLSSSSNSG-PE-LQKFRQGLDSWGCFQVINH 78
           VP RY+     R V +    D   I+I +L  +   G PE L+  R   + WG FQ++NH
Sbjct: 23  VPNRYVKPAHQRPVFNTTQSDAG-IEIPVLDMNDVWGKPEGLRLVRSACEEWGFFQMVNH 82

Query: 79  GMSSEFLEEVRQLTKQFFALPMEEKLKYSREEDDTEGYGNATILFNDQILDWTDRLFINV 138
           G++   +E VR   ++FF LP+EEK KY+   D  EGYG+   +  D  LDW+D  F+N 
Sbjct: 83  GVTHSLMERVRGAWREFFELPLEEKRKYANSPDTYEGYGSRLGVVKDAKLDWSDYFFLNY 142

Query: 139 YPEESRRLKYWPTTPERFREVADEYTENVRLLSEKIFKAMAKSLDLEDENSFVNQFVEGF 198
            P   R    WP+ P + RE+ ++Y E VR L E++ + +++SL L+  N  +     G 
Sbjct: 143 LPSSIRNPSKWPSQPPKIRELIEKYGEEVRKLCERLTETLSESLGLK-PNKLMQALGGGD 202

Query: 199 KLDAYF--NLYPQCRNPGLVLGLKPHSDASSITILLQDKEVEGLQFLKDNEWFNAPTLPD 258
           K+ A    N YP+C  P L LGL  HSD   ITILL D++V GLQ  + + W    ++P+
Sbjct: 203 KVGASLRTNFYPKCPQPQLTLGLSSHSDPGGITILLPDEKVAGLQVRRGDGWVTIKSVPN 262

Query: 259 ALLVNVGDQVEITSNGIFKSPIHRVLTNTERERISLAVFFLPDSEKEIEPLEELITETRP 318
           AL+VN+GDQ++I SNGI+KS  H+V+ N+  ER+SLA F+ P S+  + P+EEL+T  RP
Sbjct: 263 ALIVNIGDQLQILSNGIYKSVEHQVIVNSGMERVSLAFFYNPRSDIPVGPIEELVTANRP 322

Query: 319 RLYKTVK 322
            LYK ++
Sbjct: 323 ALYKPIR 327

[Close]

BLAST of Lag0034267 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 7.9e-58
Identity = 116/331 (35.05%), Postives = 189/331 (57.10%), Query Frame = 0


Query: 19  VPERYIHKESDRGVLDAPL---MDVPVIDIDLLSSSSNSGPELQKFRQGLDSWGCFQVIN 78
           VP RY+  + D+  +D      +++P+ID+  L SS+    E++K       WG FQ++N
Sbjct: 29  VPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDSEVEKLDFACKEWGFFQLVN 88

Query: 79  HGMSSEFLEEVRQLTKQFFALPMEEKLKYSREEDDTEGYGNATILFNDQILDWTDRLFIN 138
           HG+ S FL++V+   + FF LPMEEK K+ +  D+ EG+G A ++  DQ LDW D  F  
Sbjct: 89  HGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQAFVVSEDQKLDWADLFFHT 148

Query: 139 VYPEESRRLKYWPTTPERFREVADEYTENVRLLSEKIFKAMAKSLDLEDENSFVNQFVEG 198
           V P E R+   +P  P  FR+  + Y+  V+ +++ +   MA++L+++ E      F + 
Sbjct: 149 VQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKMARALEIKPE-ELEKLFDDV 208

Query: 199 FKLDAY-FNLYPQCRNPGLVLGLKPHSDASSITILLQDKEVEGLQFLKDNEWFNAPTLPD 258
             + +   N YP C  P  V+GL PHSD+  +T+L+Q  +VEGLQ  KD +W     LP+
Sbjct: 209 DSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDVEGLQIKKDGKWVPVKPLPN 268

Query: 259 ALLVNVGDQVEITSNGIFKSPIHRVLTNTERERISLAVFFLPDSEKEIEPLEELITETRP 318
           A +VN+GD +EI +NG ++S  HR + N+E+ER+S+A F      KE+ P + L+   + 
Sbjct: 269 AFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHNVGMYKEVGPAKSLVERQKV 328

Query: 319 RLYK--TVKNFAADYYQYYQKGQRPIEAARI 344
             +K  T+K +    +     G+  ++A RI
Sbjct: 329 ARFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

[Close]

BLAST of Lag0034267 vs. ExPASy Swiss-Prot
Match: Q94LP4 (2-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD11 PE=1 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 1.1e-56
Identity = 120/335 (35.82%), Postives = 190/335 (56.72%), Query Frame = 0


Query: 17  EKVPERYIHKE-SDRGVLD--APLMDVPVIDIDLLSSSSNSGPELQKFRQGLDSWGCFQV 76
           E +PERYI  E S   V++     M +P+ID+  L    +S  E  K R     WG F +
Sbjct: 27  EHIPERYIRPEASSEEVINNYQGDMAIPIIDLKKLLCPQSSEEECVKLRSACQYWGFFLL 86

Query: 77  INHGMSSEFLEEVRQLTKQFFALPMEEKLKYSREEDDTEGYGNATILFNDQILDWTDRLF 136
           INHG+  E +  +++    FF+ P++ K +Y++  +  EGYG + +   DQ LDW D L+
Sbjct: 87  INHGVPDEVIANLKRDIVDFFSQPLDTKKEYTQLPNSLEGYGQSFVFSEDQKLDWADMLY 146

Query: 137 INVYPEESRRLKYWPTTPERFREVADEYTENVRLLSEKIFKAMAKSLDLEDENSFVNQFV 196
           ++V+P +SR L++WPT+P  FR+  D Y+   + L+  +F+ MAK++  + E S ++ F 
Sbjct: 147 LHVHPSDSRDLRFWPTSPASFRQSIDAYSSETKSLALCLFEFMAKAVGAKPE-SLLDLFE 206

Query: 197 E---GFKLDAYFNLYPQCRNPGLVLGLKPHSDASSITILLQDKEVEGLQFLKDNEWFNAP 256
           E   G ++ AY   YP CR    V+GL PHSDA  +T+LL+   V+GLQ  KD +WF+  
Sbjct: 207 EQPRGLRM-AY---YPPCRQADKVMGLSPHSDAGGLTLLLEINNVQGLQIKKDGKWFSID 266

Query: 257 TLPDALLVNVGDQVEITSNGIFKSPIHRVLTNTERERISLAVFFLPDSEKEIEPLEELIT 316
               AL+ N+GD +EI SNG F+S  HR + N  +ERIS A+F  P     I PL E + 
Sbjct: 267 APNGALIANIGDTLEILSNGKFRSVEHRAVINPNKERISAALFHYPSENMVISPLPEFVK 326

Query: 317 ETRPRLYKTVK--NFAADYYQYYQKGQRPIEAARI 344
           + + + Y+++   +F    +     G+  +E  ++
Sbjct: 327 DGKVK-YRSISYLDFMKQIFTQQLDGKNRVEVLKL 355

[Close]

BLAST of Lag0034267 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 1.9e-56
Identity = 123/336 (36.61%), Postives = 184/336 (54.76%), Query Frame = 0


Query: 10  VQGVASK--EKVPERYIHKESDRGVL--DAP-LMDVPVIDIDLLSSSSNSGPE--LQKFR 69
           VQ +A      +P+RYI   S R     DAP   ++P+ID++ L S      +  + +  
Sbjct: 27  VQSLAESNLSSLPDRYIKPASLRPTTTEDAPTATNIPIIDLEGLFSEEGLSDDVIMARIS 86

Query: 70  QGLDSWGCFQVINHGMSSEFLEEVRQLTKQFFALPMEEKLKYSREEDDTEGYGNATILFN 129
           +    WG FQV+NHG+  E ++  R+  ++FF +P+  K  YS      EGYG+   +  
Sbjct: 87  EACRGWGFFQVVNHGVKPELMDAARENWREFFHMPVNAKETYSNSPRTYEGYGSRLGVEK 146

Query: 130 DQILDWTDRLFINVYPEESRRLKYWPTTPERFREVADEYTENVRLLSEKIFKAMAKSLDL 189
              LDW+D  F+++ P   +    WP+ P   REV DEY E +  LS +I + ++ +L L
Sbjct: 147 GASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIREVIDEYGEELVKLSGRIMRVLSTNLGL 206

Query: 190 EDENSFVNQF-VEGFKLDAYFNLYPQCRNPGLVLGLKPHSDASSITILLQDKEVEGLQFL 249
           + E+ F   F  E        N YP+C  P L LGL PHSD   +TILL D +V GLQ  
Sbjct: 207 K-EDKFQEAFGGENIGACLRVNYYPKCPRPELALGLSPHSDPGGMTILLPDDQVFGLQVR 266

Query: 250 KDNEWFNAPTLPDALLVNVGDQVEITSNGIFKSPIHRVLTNTERERISLAVFFLPDSEKE 309
           KD+ W      P A +VN+GDQ++I SN  +KS  HRV+ N+++ER+SLA F+ P S+  
Sbjct: 267 KDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEHRVIVNSDKERVSLAFFYNPKSDIP 326

Query: 310 IEPLEELITETRPRLYKTVKNFAADYYQYYQKGQRP 338
           I+PL+EL++   P LY  +     D Y+ + + Q P
Sbjct: 327 IQPLQELVSTHNPPLYPPM---TFDQYRLFIRTQGP 358

[Close]

BLAST of Lag0034267 vs. ExPASy Swiss-Prot
Match: D4N502 (Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 2.5e-56
Identity = 123/307 (40.07%), Postives = 171/307 (55.70%), Query Frame = 0


Query: 18  KVPERY-IHKESDRGVLDAPLMD---VPVIDI-DLLSSSSNSGP-ELQKFRQGLDSWGCF 77
           ++P RY    ES    + A + D   VPVID+ +LLS     G  EL K       WG F
Sbjct: 28  EIPSRYTCTGESPLNNIGASVTDDETVPVIDLQNLLSPEPVVGKLELDKLHSACKEWGFF 87

Query: 78  QVINHGMSSEFLEEVRQLTKQFFALPMEEKLKYSREEDDTEGYGNATILFNDQILDWTDR 137
           Q++NHG+ +  ++ ++   K FF LPM EK KY +++ D EG+G   I   DQ LDWT+ 
Sbjct: 88  QLVNHGVDALLMDNIKSEIKGFFNLPMNEKTKYGQQDGDFEGFGQPYIESEDQRLDWTEV 147

Query: 138 LFINVYPEESRRLKYWPTTPERFREVADEYTENVRLLSEKIFKAMAKSLDLEDENSFVNQ 197
             +   P   R+   +P  P  FRE  + Y   ++ LS  +F+ + KSL L +     + 
Sbjct: 148 FSMLSLPLHLRKPHLFPELPLPFRETLESYLSKMKKLSTVVFEMLEKSLQLVEIKGMTDL 207

Query: 198 FVEGFKLDAYFNLYPQCRNPGLVLGLKPHSDASSITILLQDKEVEGLQFLKDNEWFNAPT 257
           F +G +     N YP C  P LVLGL  HSD S +TILLQ  EVEGLQ  K+  W +   
Sbjct: 208 FEDGLQ-TMRMNYYPPCPRPELVLGLTSHSDFSGLTILLQLNEVEGLQIRKEERWISIKP 267

Query: 258 LPDALLVNVGDQVEITSNGIFKSPIHRVLTNTERERISLAVFFLPDSEKEIEPLEELITE 317
           LPDA +VNVGD +EI +NGI++S  HR + N+ +ER+S+A F     E EI P+  L+T 
Sbjct: 268 LPDAFIVNVGDILEIMTNGIYRSVEHRAVVNSTKERLSIATFHDSKLESEIGPISSLVTP 327

Query: 318 TRPRLYK 319
             P L+K
Sbjct: 328 ETPALFK 333

[Close]

BLAST of Lag0034267 vs. ExPASy TrEMBL
Match: A0A5A7U2M0 (Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00660 PE=3 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 3.7e-151
Identity = 262/343 (76.38%), Postives = 297/343 (86.59%), Query Frame = 0


Query: 1   MAGINPPGAVQGVASKEKVPERYIHKESDRGVLDAPLMDVPVIDIDLLSSSSNSGPELQK 60
           MA  NP G VQ VASK +VPERYIHKESDRG  +APLM  PVIDI LLSSSS SGPEL+K
Sbjct: 20  MASTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 79

Query: 61  FRQGLDSWGCFQVINHGMSSEFLEEVRQLTKQFFALPMEEKLKYSREEDDTEGYGNATIL 120
            R GL SWGCFQ INHGM+SE+L+EVRQLTKQFF L MEEKLKY +EE + EGYGN  IL
Sbjct: 80  LRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMIL 139

Query: 121 FNDQILDWTDRLFINVYPEESRRLKYWPTTPERFREVADEYTENVRLLSEKIFKAMAKSL 180
            N QILDWTDRL++ VYP +SRR KYWPT P+RFREV  EYT NV+L+SEKI KAMA+SL
Sbjct: 140 SNQQILDWTDRLYLTVYPHQSRRFKYWPTNPQRFREVVGEYTTNVKLISEKILKAMARSL 199

Query: 181 DLEDENSFVNQFVEGFKLDAYFNLYPQCRNPGLVLGLKPHSDASSITILLQDKEVEGLQF 240
           DL DE+SF+NQ+ E  KLDA FN YP+CRNP LVLG+KPH+D S+ITILLQDKEVEGLQF
Sbjct: 200 DL-DESSFLNQYGEQVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQF 259

Query: 241 LKDNEWFNAPTLPDALLVNVGDQVEITSNGIFKSPIHRVLTNTERERISLAVFFLPDSEK 300
           +KDNEW+NAP +PDALLVNVGDQVEITSNGIFKSP+HRVLTN+ERERISLAVF+LPDSEK
Sbjct: 260 MKDNEWYNAPIVPDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEK 319

Query: 301 EIEPLEELITETRPRLYKTVKNFAADYYQYYQKGQRPIEAARI 344
           EIEPLEELI E++PRLYK+VKNF   Y++YYQ+GQRP+EAARI
Sbjct: 320 EIEPLEELINESQPRLYKSVKNFVGLYFEYYQQGQRPMEAARI 361

[Close]

BLAST of Lag0034267 vs. ExPASy TrEMBL
Match: A0A5D3CIZ6 (Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold367G00230 PE=3 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 3.5e-149
Identity = 259/343 (75.51%), Postives = 295/343 (86.01%), Query Frame = 0


Query: 1   MAGINPPGAVQGVASKEKVPERYIHKESDRGVLDAPLMDVPVIDIDLLSSSSNSGPELQK 60
           MA  NP G VQ VASK +VPERYIHKESDRG  +APLM  PVIDI LLSSSS SGPEL+K
Sbjct: 20  MASTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 79

Query: 61  FRQGLDSWGCFQVINHGMSSEFLEEVRQLTKQFFALPMEEKLKYSREEDDTEGYGNATIL 120
            R GL SWGCFQ INHGM+SE+L+EVRQLTKQFF L MEEKLKY +EE + EGYGN  IL
Sbjct: 80  LRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMIL 139

Query: 121 FNDQILDWTDRLFINVYPEESRRLKYWPTTPERFREVADEYTENVRLLSEKIFKAMAKSL 180
            N QILDWTDRL++ VYP +SRR KYWP  P+RFREV  EYT NV+L+SEKI KAMA+SL
Sbjct: 140 SNQQILDWTDRLYLTVYPHQSRRFKYWPINPQRFREVVGEYTTNVKLISEKILKAMARSL 199

Query: 181 DLEDENSFVNQFVEGFKLDAYFNLYPQCRNPGLVLGLKPHSDASSITILLQDKEVEGLQF 240
           DL DE+SF+NQ+ E  KLDA FN YP+CRNP LVLG+KPH+D S+ITILLQDKEVEGLQF
Sbjct: 200 DL-DESSFLNQYGEQVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQF 259

Query: 241 LKDNEWFNAPTLPDALLVNVGDQVEITSNGIFKSPIHRVLTNTERERISLAVFFLPDSEK 300
           +KDNEW+NAP + DALLVNVGDQVEITSNGIFKSP+HRVLTN+ERERISLAVF+LPDSEK
Sbjct: 260 MKDNEWYNAPIVTDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEK 319

Query: 301 EIEPLEELITETRPRLYKTVKNFAADYYQYYQKGQRPIEAARI 344
           EIEPLEELI E++PRLYK+VKNF   Y++YYQ+G+RP+EAARI
Sbjct: 320 EIEPLEELINESQPRLYKSVKNFVGLYFEYYQQGERPMEAARI 361

[Close]

BLAST of Lag0034267 vs. ExPASy TrEMBL
Match: A0A6J1H9T6 (protein SRG1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461440 PE=3 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 3.5e-149
Identity = 261/343 (76.09%), Postives = 292/343 (85.13%), Query Frame = 0


Query: 1   MAGINPPGAVQGVASKEKVPERYIHKESDRGVLDAPLMDVPVIDIDLLSSSSNSGPELQK 60
           MAG NP G VQ VASK +VPERYIHKESDRG  DAPLM  PVID+ LLSSSS SGPEL+K
Sbjct: 20  MAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAPVIDMALLSSSSESGPELEK 79

Query: 61  FRQGLDSWGCFQVINHGMSSEFLEEVRQLTKQFFALPMEEKLKYSREEDDTEGYGNATIL 120
            R GL SWGCFQV+NHGMS+EFL+E+R+L KQFF LPMEEK KYSREED+ EGYGN  IL
Sbjct: 80  LRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEIEGYGNDMIL 139

Query: 121 FNDQILDWTDRLFINVYPEESRRLKYWPTTPERFREVADEYTENVRLLSEKIFKAMAKSL 180
            N QILDWTDRL++ VYP+ES R KYWPT PERFR V  EYT NV+LLSEKI KAMA SL
Sbjct: 140 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEYTANVKLLSEKILKAMAISL 199

Query: 181 DLEDENSFVNQFVEGFKLDAYFNLYPQCRNPGLVLGLKPHSDASSITILLQDKEVEGLQF 240
           DL +E+SF+ Q+ E  KLDA FN YP+CRNP LVLG+KPH+D S+ITILLQD+EVEGLQF
Sbjct: 200 DL-NEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQF 259

Query: 241 LKDNEWFNAPTLPDALLVNVGDQVEITSNGIFKSPIHRVLTNTERERISLAVFFLPDSEK 300
           L  NEWFNAP +P ALL+NVGDQ EITSNGIFKSP+HRVLTN+ERERISLAVF+LPD EK
Sbjct: 260 LNGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDWEK 319

Query: 301 EIEPLEELITETRPRLYKTVKNFAADYYQYYQKGQRPIEAARI 344
           EIEPLE+LI ETRPRLYKTVKNF   Y+QYYQ+GQRP+EAARI
Sbjct: 320 EIEPLEKLIDETRPRLYKTVKNFVGLYFQYYQQGQRPMEAARI 361

[Close]

BLAST of Lag0034267 vs. ExPASy TrEMBL
Match: A0A6J1JKD6 (probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485215 PE=3 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 4.5e-149
Identity = 262/343 (76.38%), Postives = 293/343 (85.42%), Query Frame = 0


Query: 1   MAGINPPGAVQGVASKEKVPERYIHKESDRGVLDAPLMDVPVIDIDLLSSSSNSGPELQK 60
           MAG NP G VQ VASK +VPERYIH ESDRG LDAPLM  PVID+ LLSS S SGPEL+K
Sbjct: 1   MAGTNPSGTVQDVASKGEVPERYIHIESDRGALDAPLMAAPVIDMALLSSPSKSGPELEK 60

Query: 61  FRQGLDSWGCFQVINHGMSSEFLEEVRQLTKQFFALPMEEKLKYSREEDDTEGYGNATIL 120
            R GL SWGCFQV+NHGMS+EFL+EVR+L KQFF LPMEEK KYSREE++ EGYGN  IL
Sbjct: 61  LRHGLQSWGCFQVVNHGMSAEFLDEVRRLAKQFFDLPMEEKSKYSREENEIEGYGNDMIL 120

Query: 121 FNDQILDWTDRLFINVYPEESRRLKYWPTTPERFREVADEYTENVRLLSEKIFKAMAKSL 180
            N QILDWTDRL++ VYP+ES R KYWPT PERFREV  EYT NV+LLSEKI KAMA SL
Sbjct: 121 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMAISL 180

Query: 181 DLEDENSFVNQFVEGFKLDAYFNLYPQCRNPGLVLGLKPHSDASSITILLQDKEVEGLQF 240
           DL +E+SF+ Q+ E  KLDA FN YP+CRNP LVLG+KPH+D S+ITILLQD+EVEGLQF
Sbjct: 181 DL-NEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQF 240

Query: 241 LKDNEWFNAPTLPDALLVNVGDQVEITSNGIFKSPIHRVLTNTERERISLAVFFLPDSEK 300
           L  NEWFNAP +P ALLVNVGDQ EITSNGIFKSP+HRVLTN+ERERISLAVF+LPDSEK
Sbjct: 241 LNGNEWFNAPIVPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEK 300

Query: 301 EIEPLEELITETRPRLYKTVKNFAADYYQYYQKGQRPIEAARI 344
           EIEPLE+LI ETRPRLYK+VKNF   Y+QYYQ+GQRP+EAARI
Sbjct: 301 EIEPLEKLIDETRPRLYKSVKNFVGLYFQYYQQGQRPMEAARI 342

[Close]

BLAST of Lag0034267 vs. ExPASy TrEMBL
Match: A0A1S3CP33 (protein SRG1-like OS=Cucumis melo OX=3656 GN=LOC103503160 PE=3 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 7.7e-149
Identity = 258/343 (75.22%), Postives = 296/343 (86.30%), Query Frame = 0


Query: 1   MAGINPPGAVQGVASKEKVPERYIHKESDRGVLDAPLMDVPVIDIDLLSSSSNSGPELQK 60
           MA  NP G VQ VASK +VPERYIHKESDRG  +APLM  PVIDI LLSSSS SGPEL+K
Sbjct: 20  MASTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 79

Query: 61  FRQGLDSWGCFQVINHGMSSEFLEEVRQLTKQFFALPMEEKLKYSREEDDTEGYGNATIL 120
            R GL SWGCFQ INHGM+SE+L+EVRQLTKQFF L MEEKLKY +EE + EGYGN  IL
Sbjct: 80  LRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMIL 139

Query: 121 FNDQILDWTDRLFINVYPEESRRLKYWPTTPERFREVADEYTENVRLLSEKIFKAMAKSL 180
            N QILDWTDRL++ VYP +SRR KYWPT P+RFREV  EYT NV+L+SEKI KAMA+SL
Sbjct: 140 SNQQILDWTDRLYLTVYPHQSRRFKYWPTNPQRFREVVGEYTTNVKLISEKILKAMARSL 199

Query: 181 DLEDENSFVNQFVEGFKLDAYFNLYPQCRNPGLVLGLKPHSDASSITILLQDKEVEGLQF 240
           DL DE+SF++Q+ E  KLDA FN YP+CRNP LVLG+KPH+D S+ITILLQDK+VEGLQF
Sbjct: 200 DL-DESSFLSQYGEQVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKKVEGLQF 259

Query: 241 LKDNEWFNAPTLPDALLVNVGDQVEITSNGIFKSPIHRVLTNTERERISLAVFFLPDSEK 300
           +KDNEW+NAP + DALLVNVGDQVEITSNGIFKSP+HRVLTN+ERERISLAVF+LPDSEK
Sbjct: 260 MKDNEWYNAPIVTDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEK 319

Query: 301 EIEPLEELITETRPRLYKTVKNFAADYYQYYQKGQRPIEAARI 344
           EIEPLEELI E++PRLYK+VKNF   Y++YYQ+G+RP+EAARI
Sbjct: 320 EIEPLEELINESQPRLYKSVKNFVGLYFEYYQQGERPMEAARI 361

[Close]

BLAST of Lag0034267 vs. TAIR 10
Match: AT5G20400.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 389.0 bits (998), Expect = 3.8e-108
Identity = 183/337 (54.30%), Postives = 249/337 (73.89%), Query Frame = 0


Query: 13  VASKEKVPERYIHKESDRGVLD-----APLMDVPVIDIDLLSSSSNSG-PELQKFRQGLD 72
           VA+ E +PERY+H  +  G +       P MD+P ID++LL SSS +G  EL K    L 
Sbjct: 13  VAAGEGLPERYLHAPTGDGEVQPLNAAVPEMDIPAIDLNLLLSSSEAGQQELSKLHSALS 72

Query: 73  SWGCFQVINHGMSSEFLEEVRQLTKQFFALPMEEKLKYSREEDDTEGYGNATILFNDQIL 132
           +WG  QV+NHG++  FL+++ +LTK+FFALP EEK K +RE D  +GYGN  IL++DQ+L
Sbjct: 73  TWGVVQVMNHGITKAFLDKIYKLTKEFFALPTEEKQKCAREIDSIQGYGNDMILWDDQVL 132

Query: 133 DWTDRLFINVYPEESRRLKYWPTTPERFREVADEYTENVRLLSEKIFKAMAKSLDLEDEN 192
           DW DRL+I  YPE+ R+L +WP  P  FRE   EYT   R++ E+ FKAMA+SL+LE EN
Sbjct: 133 DWIDRLYITTYPEDQRQLNFWPEVPLGFRETLHEYTMKQRIVIEQFFKAMARSLELE-EN 192

Query: 193 SFVNQFVEGFKLDAYFNLYPQCRNPGLVLGLKPHSDASSITILLQDKEVEGLQFLKDNEW 252
           SF++ + E   LD  FN+YP C +P  V+G+KPH+D S+IT+LL DK+V GLQF KD +W
Sbjct: 193 SFLDMYGESATLDTRFNMYPPCPSPDKVIGVKPHADGSAITLLLPDKDVGGLQFQKDGKW 252

Query: 253 FNAPTLPDALLVNVGDQVEITSNGIFKSPIHRVLTNTERERISLAVFFLPDSEKEIEPLE 312
           + AP +PD +L+NVGDQ+EI SNGI+KSP+HRV+TN E+ERIS+A F +P ++KEI+P+ 
Sbjct: 253 YKAPIVPDTILINVGDQMEIMSNGIYKSPVHRVVTNREKERISVATFCIPGADKEIQPVN 312

Query: 313 ELITETRPRLYKTVKNFAADYYQYYQKGQRPIEAARI 344
           EL++E RPRLYKTVK +   Y++YYQ+G+RPIEAA I
Sbjct: 313 ELVSEARPRLYKTVKKYVELYFKYYQQGRRPIEAALI 348

[Close]

BLAST of Lag0034267 vs. TAIR 10
Match: AT1G49390.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 371.3 bits (952), Expect = 8.3e-103
Identity = 177/337 (52.52%), Postives = 240/337 (71.22%), Query Frame = 0


Query: 13  VASKEKVPERYIHKESDRGVLD-----APLMDVPVIDIDLLSSSSNSG-PELQKFRQGLD 72
           VA+ + +PERY+H  +  G         P MD+P ID+ LL SSS  G  E++K    L 
Sbjct: 13  VAAGQGLPERYLHAPTGEGESQPLNGAVPEMDIPAIDLSLLFSSSVDGQEEMKKLHSALS 72

Query: 73  SWGCFQVINHGMSSEFLEEVRQLTKQFFALPMEEKLKYSREEDDTEGYGNATILFNDQIL 132
           +WG  QV+NHG++  FL+++ +LTKQFFALP EEK K +RE  + +GYGN  IL ++Q+L
Sbjct: 73  TWGVVQVMNHGITEAFLDKIYKLTKQFFALPTEEKHKCARETGNIQGYGNDMILSDNQVL 132

Query: 133 DWTDRLFINVYPEESRRLKYWPTTPERFREVADEYTENVRLLSEKIFKAMAKSLDLEDEN 192
           DW DRLF+  YPE+ R+LK+WP  P  F E  DEYT   R+L EK FKAMA+SL+LE EN
Sbjct: 133 DWIDRLFLTTYPEDKRQLKFWPQVPVGFSETLDEYTMKQRVLIEKFFKAMARSLELE-EN 192

Query: 193 SFVNQFVEGFKLDAYFNLYPQCRNPGLVLGLKPHSDASSITILLQDKEVEGLQFLKDNEW 252
            F+  + E   +++ FN +P C  P  V+G+KPH+D S+IT+LL DK+VEGLQFLKD +W
Sbjct: 193 CFLEMYGENAVMNSRFNFFPPCPRPDKVIGIKPHADGSAITLLLPDKDVEGLQFLKDGKW 252

Query: 253 FNAPTLPDALLVNVGDQVEITSNGIFKSPIHRVLTNTERERISLAVFFLPDSEKEIEPLE 312
           + AP +PD +L+ +GDQ+EI SNGI+KSP+HRV+TN E+ERIS+A F +P  +KEI P +
Sbjct: 253 YKAPIVPDTILITLGDQMEIMSNGIYKSPVHRVVTNREKERISVATFCVPGLDKEIHPAD 312

Query: 313 ELITETRPRLYKTVKNFAADYYQYYQKGQRPIEAARI 344
            L+TE RPRLYKTV  +   +Y+YYQ+G+R IEAA I
Sbjct: 313 GLVTEARPRLYKTVTKYVDLHYKYYQQGRRTIEAALI 348

[Close]

BLAST of Lag0034267 vs. TAIR 10
Match: AT5G54000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 359.8 bits (922), Expect = 2.5e-99
Identity = 172/338 (50.89%), Postives = 233/338 (68.93%), Query Frame = 0


Query: 13  VASKEKVPERYIHKESDRGVLDAPL------MDVPVIDIDLLSSSSNSG-PELQKFRQGL 72
           VA+ EK+PERY++  +  G  D P       M + +ID++LL SSS+ G  EL K    +
Sbjct: 13  VAAGEKLPERYLYTPTGDGEGDQPFNGLLPEMKISIIDLNLLFSSSDDGREELSKLHSAI 72

Query: 73  DSWGCFQVINHGMSSEFLEEVRQLTKQFFALPMEEKLKYSREEDDTEGYGNATILFNDQI 132
            +WG  QV+NHG+S   L+++ +LTKQFF LP +EK KY+RE    +G+GN  IL +DQ+
Sbjct: 73  STWGVVQVMNHGISEALLDKIHELTKQFFVLPTKEKQKYAREISSFQGFGNDMILSDDQV 132

Query: 133 LDWTDRLFINVYPEESRRLKYWPTTPERFREVADEYTENVRLLSEKIFKAMAKSLDLEDE 192
           LDW DRL++  YPE+ R+LK+WP  P  FRE   EYT   +L+ EK FKA+A+SL+LED 
Sbjct: 133 LDWVDRLYLITYPEDQRQLKFWPENPSGFRETLHEYTMKQQLVVEKFFKALARSLELED- 192

Query: 193 NSFVNQFVEGFKLDAYFNLYPQCRNPGLVLGLKPHSDASSITILLQDKEVEGLQFLKDNE 252
           N F+    E   L+  FN+YP C  P  VLGLKPHSD S+ T++L DK VEGLQFLKD +
Sbjct: 193 NCFLEMHGENATLETRFNIYPPCPRPDKVLGLKPHSDGSAFTLILPDKNVEGLQFLKDGK 252

Query: 253 WFNAPTLPDALLVNVGDQVEITSNGIFKSPIHRVLTNTERERISLAVFFLPDSEKEIEPL 312
           W+ A  LP  +L+NVGD +E+ SNGI+KSP+HRV+ N ++ERI +A F   D +KEI+PL
Sbjct: 253 WYKASILPHTILINVGDTMEVMSNGIYKSPVHRVVLNGKKERIYVATFCNADEDKEIQPL 312

Query: 313 EELITETRPRLYKTVKNFAADYYQYYQKGQRPIEAARI 344
             L++E RPRLYK VK    +++ YYQ+G+RPIEAA I
Sbjct: 313 NGLVSEARPRLYKAVKKSEKNFFDYYQQGRRPIEAAMI 349

[Close]

BLAST of Lag0034267 vs. TAIR 10
Match: AT5G20550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 357.8 bits (917), Expect = 9.5e-99
Identity = 177/338 (52.37%), Postives = 240/338 (71.01%), Query Frame = 0


Query: 13  VASKEKVPERYIHKES--DRGV-LDA--PLMDVPVIDIDLLSSSSNSG-PELQKFRQGLD 72
           VA+ E +PERY+   +  D G  L+A  P+MD+P ID+ LL S S+ G  EL K    L 
Sbjct: 13  VAAGEGIPERYLQPPAVDDNGQHLNAAVPVMDIPAIDLSLLLSPSDDGREELSKLHSALS 72

Query: 73  SWGCFQVINHGMSSEFLEEVRQLTKQFFALPMEEKLKYSREEDDTEGYGNATILFNDQIL 132
           +WG  QVINHG++   L+++ +LTK+F ALP EEK KY+RE    +GYGN  IL++DQ+L
Sbjct: 73  TWGVVQVINHGITKALLDKIYKLTKEFCALPSEEKQKYAREIGSIQGYGNDMILWDDQVL 132

Query: 133 DWTDRLFINVYPEESRRLKYWPTTPERFREVADEYTENVRLLSEKIFKAMAKSLDLEDEN 192
           DW DRL+I  YPE+ R+LK+WP  P  FRE   EYT    L+  ++FKAMA SL+LE EN
Sbjct: 133 DWIDRLYITTYPEDQRQLKFWPDVPVGFRETLHEYTMKQHLVFNQVFKAMAISLELE-EN 192

Query: 193 SFVNQFVEGFKLDAYFNLYPQCRNPGLVLGLKPHSDASSITILLQDKEVEGLQFLKDNEW 252
            F++   E   +D  FN+YP C  P  V+G++PH+D S+ T+LL DK VEGLQFLKD +W
Sbjct: 193 CFLDMCGENATMDTRFNMYPPCPRPDKVIGVRPHADKSAFTLLLPDKNVEGLQFLKDGKW 252

Query: 253 FNAPTL-PDALLVNVGDQVEITSNGIFKSPIHRVLTNTERERISLAVFFLPDSEKEIEPL 312
           + AP +  D +L+NVGDQ+EI SNGI+KSP+HRV+TNTE+ERIS+A F +P ++KEI+P+
Sbjct: 253 YKAPVVASDTILINVGDQMEIMSNGIYKSPVHRVVTNTEKERISVATFCIPGADKEIQPV 312

Query: 313 EELITETRPRLYKTVKNFAADYYQYYQKGQRPIEAARI 344
           + L++E RPRLYK VKN+     +YY +GQRPI A+ I
Sbjct: 313 DGLVSEARPRLYKPVKNYVDLLNKYYIQGQRPIAASLI 349

[Close]

BLAST of Lag0034267 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 262.3 bits (669), Expect = 5.4e-70
Identity = 139/340 (40.88%), Postives = 204/340 (60.00%), Query Frame = 0


Query: 18  KVPERYIHKESDRGVLDAPLM------DVPVIDIDLLSSSSNSG--PELQKFRQGLDSWG 77
           KVPER+I +E +RGV+ + L        +PVID+  LS   N     E+ K  Q  + WG
Sbjct: 27  KVPERFIREEYERGVVVSSLKTHHLHHQIPVIDLSKLSKPDNDDFFFEILKLSQACEDWG 86

Query: 78  CFQVINHGMSSEFLEEVRQLTKQFFALPMEEKLKYSREEDDTEGYGNATILFNDQILDWT 137
            FQVINHG+  E +E++ ++  +FF +P+EEK KY  E    +GYG A I   DQ LDW 
Sbjct: 87  FFQVINHGIEVEVVEDIEEVASEFFDMPLEEKKKYPMEPGTVQGYGQAFIFSEDQKLDWC 146

Query: 138 DRLFINVYPEESRRLKYWPTTPERFREVADEYTENVRLLSEKIFKAMAKSLDLEDENSFV 197
           +   + V+P + R  K WP+ P RF E  + Y++ +R L +++ K +A SL L++E  F 
Sbjct: 147 NMFALGVHPPQIRNPKLWPSKPARFSESLEGYSKEIRELCKRLLKYIAISLGLKEER-FE 206

Query: 198 NQFVEGFKLDAYFNLYPQCRNPGLVLGLKPHSDASSITILLQDK-EVEGLQFLKDNEWFN 257
             F E  +     N YP C +P LVLGL PHSD S++T+L Q K    GLQ LKDN W  
Sbjct: 207 EMFGEAVQA-VRMNYYPPCSSPDLVLGLSPHSDGSALTVLQQSKNSCVGLQILKDNTWVP 266

Query: 258 APTLPDALLVNVGDQVEITSNGIFKSPIHRVLTNTERERISLAVFFLPDSEKEIEPLEEL 317
              LP+AL++N+GD +E+ SNG +KS  HR +TN E+ER+++  F+ P+ E EIEP+ EL
Sbjct: 267 VKPLPNALVINIGDTIEVLSNGKYKSVEHRAVTNREKERLTIVTFYAPNYEVEIEPMSEL 326

Query: 318 I-TETRPRLYKTVKNFAADYYQYY----QKGQRPIEAARI 344
           +  ET P  Y++  +   DY  +Y     +G++ ++ A+I
Sbjct: 327 VDDETNPCKYRSYNH--GDYSYHYVSNKLQGKKSLDFAKI 362

The following BLAST results are available for this feature:

BLAST of Lag0034267 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0050082.1	7.7e-151	76.38	protein SRG1-like [Cucumis melo var. makuwa]	[more]
XP_011655688.2	6.5e-150	76.38	probable 2-oxoglutarate-dependent dioxygenase ANS [Cucumis sativus] >KAE8653027....	[more]
XP_023516521.1	1.4e-149	76.68	protein SRG1-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
TYK10346.1	7.2e-149	75.51	protein SRG1-like [Cucumis melo var. makuwa]	[more]
XP_022960723.1	7.2e-149	76.09	protein SRG1-like isoform X1 [Cucurbita moschata]	[more]

BLAST of Lag0034267 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80449	1.8e-62	40.72	Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1	[more]
Q39224	7.9e-58	35.05	Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1	[more]
Q94LP4	1.1e-56	35.82	2-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947...	[more]
Q9FFF6	1.9e-56	36.61	Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1	[more]
D4N502	2.5e-56	40.07	Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1	[more]

BLAST of Lag0034267 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U2M0	3.7e-151	76.38	Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00...	[more]
A0A5D3CIZ6	3.5e-149	75.51	Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold367G00...	[more]
A0A6J1H9T6	3.5e-149	76.09	protein SRG1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461440 PE=3 ...	[more]
A0A6J1JKD6	4.5e-149	76.38	probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 OS=Cucurbita maxima...	[more]
A0A1S3CP33	7.7e-149	75.22	protein SRG1-like OS=Cucumis melo OX=3656 GN=LOC103503160 PE=3 SV=1	[more]

BLAST of Lag0034267 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20400.1	3.8e-108	54.30	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G49390.1	8.3e-103	52.52	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G54000.1	2.5e-99	50.89	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G20550.1	9.5e-99	52.37	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G21420.1	5.4e-70	40.88	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 201..295 e-value: 5.4E-23 score: 81.4
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 40..148 e-value: 1.7E-24 score: 86.8
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 10..335 e-value: 9.4E-91 score: 306.5
None	No IPR available	PANTHER	PTHR47991:SF25	SUBFAMILY NOT NAMED	coord: 9..340
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 9..340
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 15..333
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 196..296 score: 13.354265

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0034267.1	Lag0034267.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding