Lag0034266 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0034266
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Locationchr3: 5831932 .. 5836629 (+)
RNA-Seq ExpressionLag0034266
SyntenyLag0034266
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCACTGAACGCATCCGTAACGAAGTTGGCACTTGTAATCCTGGTTTGCATAGCCCTTTTAGCATCACCTTCAATGGCCCGCATGGCGGTGATGGCGGCAGCACCTGATCCGTTGGTAACAGAGGATTTCTCTTTGTTCCCTTGTATATCATCTGTTCCGGAGGTGACGAAATGCATGATAGATGTGTTTAGAAATGCAGTTGCTCCTCATCCTTCATGTTGTACAGCCATTTCTAAGCTCAAAGGTTGTTCTTCTGAGTTTCTCAAAGATATTCCCTCTGTTGACATGATTTTGATTAAGAGCATTTGTGCTTTGTGGGGTGTTTCAATTTCCTAGACTAAAAGTTCTCTAGATTATAATTGGATCTTGATGAATAAGAAAGTTATATGATGATAATAAGTTTTTTTTTTCCCTTTTTTTCTACAGTATTATGTAATAAATTTATGACGATTAGTAAATTTCGTTGAAGCAAAATGTTCTCCCCTTTTAACGAGAAAGAGCATTTTCCCACAATTTGTTGTCCATTTGTTTTCTGAAGAATTTTCCATGATTGATTTCTAAATCAAGAAGATGGCCGAAGGAAGAAAAATGGGTAATTATCAAATACATTAAAAAAAATTAAATTATAAATTTAGTTCTCCGTATCATCTCAATTTGTTTTTATAATATGGGCATAGTCTTGATTAGATTCTTGTAATCTTATAAGTTTGAATTTGGTACCATTAGGCTTTGTTTCAATTTGGATTCTTATAGTTTTAAAATTTTCAATTCGATTCCCTATAGTTAATTTGAATAATAAGTTTACCAATTGTGCTAAATTGCCTTTCCCTCAACTTGAAAAACTTGCCTATTTTCTTCAAATTTGTACTCAAGCATGAACTTTGACATTGTTAACCAACATAATTAGACAAATTGATATTTATGATATATCTTTTTTACAATGCGGAGAATACTTATTTACATGATTCAACAATGGAAAATGACTAAGTTTTTTACCGCTAGTAAATAAATTTAAGGAAAATTGCACGAGGTGACCATTAGTGTTTTCGTAATTGCAAATGTTATAGGCAATTTTGGACCACCCCGATATACAAGGAGCTGACGAGGACAACCGGGGAGAAATCGGGCTGAAAGATGGACCAAGGAGGCAAAACCGGCAAATGGGACGGGCCAAGACCGAAGGGGTCGGGTTTTTGGCCCGACCCCCTGCTCGGCCCGCAGGGGTCGGAGCTGACGAGGACAACCGGGGAGAAATCGGGCTGAAAGATGGACCAAGGAGGCAAAACCGGCAAATGGGACGGGCCAAGACCGAAGGGGTCGGGTGTTCAACGAACCCGGTAACCCGAAAATCCGGCCAACCCAACCCTAATCATAAGGGTTGGGTTAGGTGAGAATTAAATTTGGGTTGGGTTGAGTTTTATTTTAAAAGAACCAATCGGTTTGGTTCTGGTGTCAGGTTCAAGGATTTCTTTTTTGGGTTGAGTAGGGATTTGATTGTCCGATTTTGAAACATATGAACCTATTGCAAACGAATCTGAAACATGAGGGACCAAAAGGGTATTTTACCCTTTCTAATACTAGTTGAGCATGACGTGGAACAAATTTTAGCCTCTGATTTTGCTATATGTGCAAGTTCCTAAAAAACATCTATCTTCATTCATCACCCATTTATTGTAATAAAAACCACATTAATCTCCGGCTTATCACTAATATATACATAGAGGTAAAACAAAAAATATATGTTTTTCTATTCACTTATCTTATTTATACGTTTGAAAAGAGAAGATTATAAGCAGTTGCGAAGTATGTTTGAGTTTCTCAATCTCTCCTCATTGACACCCCAAAGTAAAGCAAGCTGTTGCGTTGGAGTGCAAGAGAGCCGAGTTTGGTTATAAGGCTTCCTACAATATAATATCTAACATAATTGATTCCTCTGCCATTGAATTGCAAGAAGTTCGGCTGGAATCCAGAAACCTATGTCTGTTAGTTTAGTGATCCAATATAATATTTAACTGTTTCCAAAAACTGCCACAGTTTTCAACAAAAATAAAATAAGTTTATGATGCAAAGTTGTGATGAGGCACCGGAATATTTTAATGAAATTTTATTTTTAAGTTTTTAACACACCTCTAAAACAACAAAAACAAACAAACAAATAATAATAATAATAACAAAATATGATATGCACACATCCAGAGACAATAAAATATGGCAAACTACTTTTCTTACAACCTCTTTCTTATACTAGTAAGTAACACATGCAATACATGTGGAATCCACGTTTGTCGTGTTTCTCTGATTATAATTTTATAATTAAGGCTCAGATTCAACTTTCAGTATTTCTCGAGATTTGCAATGCACATGGAATCCACGTTTGTCGTGTCTCTCTTATTATAATTTTATAAGGCTCAGATTCAACTCTCTGTATTTATTGTTGTGGCTTGTTCTTTCAAATTCAAATATTCTACCAGAGAAATCCAATTTTAGTTCCTAATCATTTCCATCCTCTCTGTTGATCTCTGTTTTGCAATCAGAACTGCTCAGTGCCACCATTTCTGCAACATCGGCGATGGCTGGAATTAACCCGTCCGGCGCCGTCCAAGACGTGGCTTCTAAAGGGGAAGTGCCGGAAAGATACATCCATAAAGAAAGCGATCGAGGAGCTCTAGATGCTCCTTTAATGGAAGCTTCTATAATTGACATCGATCTCCTCTCGTCTTCATCCAATTCCGGACCGGAACTCCAGAAATTCCGACATGGACTTCAGTCATGGGGCTGCTTTCTGGTTAGATTTCAGTGAACTTAAAACCCTAATTTCAATCGGATTGTATGGCGATTTCTTTTAAACCCTGGTGTGTTTGGAACCTCATGATTTGAGGATTTCAAATCAATTCTGATGTTTGTTATCACAATAAATAGAATGATTTGAAATTTGAAATGATTTCAAGTCATATTTTTATGTCAAAAATTTTCTCAACTAAGGGTGTATTTGGTTTAACTTTTCAAGGGTTTAATTTTGAAAAAAAAAAAATCATTTTGAAAAAAATTCTAGTGTTTGACAACCACTAAAAATGACTTATTAAAAATGAGATTGAGAAATAATTCATTTTGAGACAAACATTTGAAAGCAACTTTTTTAACCAGAGTGGTTAATTATATAGTAACTGTGGTATAAAAACACTTATGAACGAGTGTAACCAAACATATTAGTTTTTAAATTTTAAACCATTTTTATGCAAATATTTATAATAAAATGCATTCTTTAAAAACACTCAAATTAAAAGGTATTCCAAACGCACCCTAAAACTATGAGATTGGGTGAGATTTCATAGGATTTAGAACTAAATTACTTATTTAATCTTGTCATATTTTAATCTTGTATAGTAAATCAATATTAAAAACCTATATTAGAAAACTTCTTACTTTCTTTTCTTTTCTTTTGTTGTTACCATCCAAACACAAAGTTTTAAATACATTCGTTTTAAATCTTATTTGTTCTAAGCAAGACAACAAATTTAAAATCATTTCTTGAACTTTCCTAATTCCAAACGGAAGGCAATTATTTCAGTGGACTCTACATCTCGATTCCAGAAAAAACGTTTGGACATTGTAAATTGCTTGAAATCAGACTCAAAAAACGTCGGGATTTTGTTATCCTAATGTTTTGAGAAGATGATCGAAAGTTTTCTGGTGTTGTTTCAGGCGAAAAATCATGGAATGTCCTCTGAACTTCTGGAAGAAGTTCGGCAAGTAATGAAAGAGTTCTTTGCTCTTCCAATGGAAGAAAAATTGAAATGCTCGAGAGAAGAAGATGACACTGAAGGATATGGCAACGCAATGATTCTCTCCAACGACCAAGTTCTGGATTGGACTGATCGTTTGAACCTCACTCTTTATCCAGAAGAGAGCCGCCGTTTGAAGTACTGGCCAACAACTCCTGAAAGATTCAGGTAATTTTTTATTTGGTTTTTATTATGAGTAATGTACAAGAATAATCTGTACAAACAAACAATTGGTTTCCAAACATTGCAGGGAAGTTATTTATGAGTACACTGAAAATGTGAGGGCGCTATGTGAGAAAATCCTCAAGGCCATGGCAAGGTCATTGGATTTGGATGAGAATAGCTTTGTCAACGAGTTTGTCGACGGATATAAACTCGCATATTTCAACTTCTACCCTCCATGTCCAAATCCCGATCTCGTTCTGGGTCTCAAGCCGCACTCCGATGGATCATCAATCACCATTCTGTTGCAGGACAAGGAAGTAGAAGGTCTTCAGATCTTGAAAGGCAATGAGTGGTTCAATGCTCCAATTGTTCCTGATGCTCTTCTCGTCCTTGTTGGGGATCAAGGAGAGGTAAACACTACTCTTTACAAAAAGTAGATATAACTAAATCACTTTAGCTAGCTAAAACCACTTATTCTAAAATCACTTGGGTTGACTTGAAATTTGTGTAGATAACAAGTAATGGGATATTCAAGAGTGTAGTTCATAGGGTATTGACAAACACAGAGAGGGAGAGGATTTCATTGGCCACTCTTTACCTTCCACATTCAGAGAAAGAAATTGAACCACTTGAGAAGCTCATCAATGAAACTCGGCCAAGGTTGTACAAGACTGTCAAGAACTTTGCTTCCCTTTACTATCAGTACTACCAGAAAGGCCAGAGGCCAATCGAGGCTGCAAGAATCTAG

mRNA sequence

ATGTCACTGAACGCATCCGTAACGAAGTTGGCACTTGTAATCCTGGTTTGCATAGCCCTTTTAGCATCACCTTCAATGGCCCGCATGGCGGTGATGGCGGCAGCACCTGATCCGTTGGTAACAGAGGATTTCTCTTTGTTCCCTTGTATATCATCTGTTCCGGAGGTGACGAAATGCATGATAGATGTGTTTAGAAATGCAGTTGCTCCTCATCCTTCATGTTGTACAGCCATTTCTAAGCTCAAAGGTTGTTCTTCTGAGTTTCTCAAAGATATTCCCTCTGTTGACATGATTTTGATTAAGAGCATTTGTGCTTTGTGGGGTGCAATTTTGGACCACCCCGATATACAAGGAGCTGACGAGGACAACCGGGGAGAAATCGGGCTGAAAGATGGACCAAGGAGGCAAAACCGGCAAATGGGACGGGCCAAGACCGAAGGGGTCGGGTTTTTGGCCCGACCCCCTGCTCGGCCCGCAGGGGTCGGAGCTGACGAGGACAACCGGGGAGAAATCGGGCTGAAAGATGGACCAAGGAGGCAAAACCGGCAAATGGGACGGGCCAAGACCGAAGGGGTCGGGTGTTCAACGAACCCGGTAACCCGAAAATCCGGCCAACCCAACCCTAATCATAAGGGTTGGGTTAGTGCCACCATTTCTGCAACATCGGCGATGGCTGGAATTAACCCGTCCGGCGCCGTCCAAGACGTGGCTTCTAAAGGGGAAGTGCCGGAAAGATACATCCATAAAGAAAGCGATCGAGGAGCTCTAGATGCTCCTTTAATGGAAGCTTCTATAATTGACATCGATCTCCTCTCGTCTTCATCCAATTCCGGACCGGAACTCCAGAAATTCCGACATGGACTTCAGTCATGGGGCTGCTTTCTGGCGAAAAATCATGGAATGTCCTCTGAACTTCTGGAAGAAGTTCGGCAAGTAATGAAAGAGTTCTTTGCTCTTCCAATGGAAGAAAAATTGAAATGCTCGAGAGAAGAAGATGACACTGAAGGATATGGCAACGCAATGATTCTCTCCAACGACCAAGTTCTGGATTGGACTGATCGTTTGAACCTCACTCTTTATCCAGAAGAGAGCCGCCGTTTGAAGTACTGGCCAACAACTCCTGAAAGATTCAGGGAAGTTATTTATGAGTACACTGAAAATGTGAGGGCGCTATGTGAGAAAATCCTCAAGGCCATGGCAAGGTCATTGGATTTGGATGAGAATAGCTTTGTCAACGAGTTTGTCGACGGATATAAACTCGCATATTTCAACTTCTACCCTCCATGTCCAAATCCCGATCTCGTTCTGGGTCTCAAGCCGCACTCCGATGGATCATCAATCACCATTCTGTTGCAGGACAAGGAAGTAGAAGGTCTTCAGATCTTGAAAGGCAATGAGTGGTTCAATGCTCCAATTGTTCCTGATGCTCTTCTCGTCCTTGTTGGGGATCAAGGAGAGATAACAAGTAATGGGATATTCAAGAGTGTAGTTCATAGGGTATTGACAAACACAGAGAGGGAGAGGATTTCATTGGCCACTCTTTACCTTCCACATTCAGAGAAAGAAATTGAACCACTTGAGAAGCTCATCAATGAAACTCGGCCAAGGTTGTACAAGACTGTCAAGAACTTTGCTTCCCTTTACTATCAGTACTACCAGAAAGGCCAGAGGCCAATCGAGGCTGCAAGAATCTAG

Coding sequence (CDS)

ATGTCACTGAACGCATCCGTAACGAAGTTGGCACTTGTAATCCTGGTTTGCATAGCCCTTTTAGCATCACCTTCAATGGCCCGCATGGCGGTGATGGCGGCAGCACCTGATCCGTTGGTAACAGAGGATTTCTCTTTGTTCCCTTGTATATCATCTGTTCCGGAGGTGACGAAATGCATGATAGATGTGTTTAGAAATGCAGTTGCTCCTCATCCTTCATGTTGTACAGCCATTTCTAAGCTCAAAGGTTGTTCTTCTGAGTTTCTCAAAGATATTCCCTCTGTTGACATGATTTTGATTAAGAGCATTTGTGCTTTGTGGGGTGCAATTTTGGACCACCCCGATATACAAGGAGCTGACGAGGACAACCGGGGAGAAATCGGGCTGAAAGATGGACCAAGGAGGCAAAACCGGCAAATGGGACGGGCCAAGACCGAAGGGGTCGGGTTTTTGGCCCGACCCCCTGCTCGGCCCGCAGGGGTCGGAGCTGACGAGGACAACCGGGGAGAAATCGGGCTGAAAGATGGACCAAGGAGGCAAAACCGGCAAATGGGACGGGCCAAGACCGAAGGGGTCGGGTGTTCAACGAACCCGGTAACCCGAAAATCCGGCCAACCCAACCCTAATCATAAGGGTTGGGTTAGTGCCACCATTTCTGCAACATCGGCGATGGCTGGAATTAACCCGTCCGGCGCCGTCCAAGACGTGGCTTCTAAAGGGGAAGTGCCGGAAAGATACATCCATAAAGAAAGCGATCGAGGAGCTCTAGATGCTCCTTTAATGGAAGCTTCTATAATTGACATCGATCTCCTCTCGTCTTCATCCAATTCCGGACCGGAACTCCAGAAATTCCGACATGGACTTCAGTCATGGGGCTGCTTTCTGGCGAAAAATCATGGAATGTCCTCTGAACTTCTGGAAGAAGTTCGGCAAGTAATGAAAGAGTTCTTTGCTCTTCCAATGGAAGAAAAATTGAAATGCTCGAGAGAAGAAGATGACACTGAAGGATATGGCAACGCAATGATTCTCTCCAACGACCAAGTTCTGGATTGGACTGATCGTTTGAACCTCACTCTTTATCCAGAAGAGAGCCGCCGTTTGAAGTACTGGCCAACAACTCCTGAAAGATTCAGGGAAGTTATTTATGAGTACACTGAAAATGTGAGGGCGCTATGTGAGAAAATCCTCAAGGCCATGGCAAGGTCATTGGATTTGGATGAGAATAGCTTTGTCAACGAGTTTGTCGACGGATATAAACTCGCATATTTCAACTTCTACCCTCCATGTCCAAATCCCGATCTCGTTCTGGGTCTCAAGCCGCACTCCGATGGATCATCAATCACCATTCTGTTGCAGGACAAGGAAGTAGAAGGTCTTCAGATCTTGAAAGGCAATGAGTGGTTCAATGCTCCAATTGTTCCTGATGCTCTTCTCGTCCTTGTTGGGGATCAAGGAGAGATAACAAGTAATGGGATATTCAAGAGTGTAGTTCATAGGGTATTGACAAACACAGAGAGGGAGAGGATTTCATTGGCCACTCTTTACCTTCCACATTCAGAGAAAGAAATTGAACCACTTGAGAAGCTCATCAATGAAACTCGGCCAAGGTTGTACAAGACTGTCAAGAACTTTGCTTCCCTTTACTATCAGTACTACCAGAAAGGCCAGAGGCCAATCGAGGCTGCAAGAATCTAG

Protein sequence

MSLNASVTKLALVILVCIALLASPSMARMAVMAAAPDPLVTEDFSLFPCISSVPEVTKCMIDVFRNAVAPHPSCCTAISKLKGCSSEFLKDIPSVDMILIKSICALWGAILDHPDIQGADEDNRGEIGLKDGPRRQNRQMGRAKTEGVGFLARPPARPAGVGADEDNRGEIGLKDGPRRQNRQMGRAKTEGVGCSTNPVTRKSGQPNPNHKGWVSATISATSAMAGINPSGAVQDVASKGEVPERYIHKESDRGALDAPLMEASIIDIDLLSSSSNSGPELQKFRHGLQSWGCFLAKNHGMSSELLEEVRQVMKEFFALPMEEKLKCSREEDDTEGYGNAMILSNDQVLDWTDRLNLTLYPEESRRLKYWPTTPERFREVIYEYTENVRALCEKILKAMARSLDLDENSFVNEFVDGYKLAYFNFYPPCPNPDLVLGLKPHSDGSSITILLQDKEVEGLQILKGNEWFNAPIVPDALLVLVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKEIEPLEKLINETRPRLYKTVKNFASLYYQYYQKGQRPIEAARI
Homology
BLAST of Lag0034266 vs. NCBI nr
Match: XP_022134811.1 (uncharacterized protein LOC111006992 [Momordica charantia])

HSP 1 Score: 539.7 bits (1389), Expect = 3.1e-149
Identity = 268/342 (78.36%), Postives = 296/342 (86.55%), Query Frame = 0

Query: 224 MAGINPSGAVQDVASKGEVPERYIHKESDRGALDAPLMEASIIDIDLLSSSSNSGPELQK 283
           MAG NP+G+VQDVASKGEVPERYIHKE DRGALDAPLMEA +IDI LLSS SN+GPEL+K
Sbjct: 1   MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK 60

Query: 284 FRHGLQSWGCFLAKNHGMSSELLEEVRQVMKEFFALPMEEKLKCSREEDDTEGYGNAMIL 343
            RHGL SWGCF A NHGMS E LEEVRQV K FFALPME+KLK SREED  EGYGN MI 
Sbjct: 61  LRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIF 120

Query: 344 SNDQVLDWTDRLNLTLYPEESRRLKYWPTTPERFREVIYEYTENVRALCEKILKAMARSL 403
           SN Q+LDWTDRL LT+ PEESRR KYWPT PERFREV++EYT NV+ L EKILKAMARSL
Sbjct: 121 SNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSL 180

Query: 404 DLDENSFVNEFVDGYKL-AYFNFYPPCPNPDLVLGLKPHSDGSSITILLQDKEVEGLQIL 463
           DLDENSF+N++    +L A FNFY  C NP+LVLG+KPH+DGS+ITILLQDKEVEGLQ L
Sbjct: 181 DLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL 240

Query: 464 KGNEWFNAPIVPDALLVLVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKE 523
           KGNEW+NAPI+PDALLV VGDQGEITSNGIFKS VHRVLTN+ERERISLA  YLP  +KE
Sbjct: 241 KGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKE 300

Query: 524 IEPLEKLINETRPRLYKTVKNFASLYYQYYQKGQRPIEAARI 565
           IEPLEKLINET PRLY+TVKNF  L++QYYQ+GQRP EAA+I
Sbjct: 301 IEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKI 342

BLAST of Lag0034266 vs. NCBI nr
Match: XP_022960723.1 (protein SRG1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 536.2 bits (1380), Expect = 3.4e-148
Identity = 265/351 (75.50%), Postives = 299/351 (85.19%), Query Frame = 0

Query: 215 SATISATSAMAGINPSGAVQDVASKGEVPERYIHKESDRGALDAPLMEASIIDIDLLSSS 274
           ++  SA  AMAG NPSG VQDVASKGEVPERYIHKESDRGA DAPLM A +ID+ LLSSS
Sbjct: 11  NSVASAPPAMAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAPVIDMALLSSS 70

Query: 275 SNSGPELQKFRHGLQSWGCFLAKNHGMSSELLEEVRQVMKEFFALPMEEKLKCSREEDDT 334
           S SGPEL+K RHGLQSWGCF   NHGMS+E L+E+R++ K+FF LPMEEK K SREED+ 
Sbjct: 71  SESGPELEKLRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEI 130

Query: 335 EGYGNAMILSNDQVLDWTDRLNLTLYPEESRRLKYWPTTPERFREVIYEYTENVRALCEK 394
           EGYGN MILSN Q+LDWTDRL LT+YP+ES R KYWPT PERFR V++EYT NV+ L EK
Sbjct: 131 EGYGNDMILSNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEYTANVKLLSEK 190

Query: 395 ILKAMARSLDLDENSFVNEFVDGYKL-AYFNFYPPCPNPDLVLGLKPHSDGSSITILLQD 454
           ILKAMA SLDL+E+SF+ ++ +  KL A FNFYP C NPDLVLG+KPH+DGS+ITILLQD
Sbjct: 191 ILKAMAISLDLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQD 250

Query: 455 KEVEGLQILKGNEWFNAPIVPDALLVLVGDQGEITSNGIFKSVVHRVLTNTERERISLAT 514
           +EVEGLQ L GNEWFNAPIVP ALL+ VGDQ EITSNGIFKS VHRVLTN+ERERISLA 
Sbjct: 251 EEVEGLQFLNGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTNSERERISLAV 310

Query: 515 LYLPHSEKEIEPLEKLINETRPRLYKTVKNFASLYYQYYQKGQRPIEAARI 565
            YLP  EKEIEPLEKLI+ETRPRLYKTVKNF  LY+QYYQ+GQRP+EAARI
Sbjct: 311 FYLPDWEKEIEPLEKLIDETRPRLYKTVKNFVGLYFQYYQQGQRPMEAARI 361

BLAST of Lag0034266 vs. NCBI nr
Match: KAA0050082.1 (protein SRG1-like [Cucumis melo var. makuwa])

HSP 1 Score: 533.9 bits (1374), Expect = 1.7e-147
Identity = 264/351 (75.21%), Postives = 301/351 (85.75%), Query Frame = 0

Query: 215 SATISATSAMAGINPSGAVQDVASKGEVPERYIHKESDRGALDAPLMEASIIDIDLLSSS 274
           +A +S   AMA  NPSG VQDVASKGEVPERYIHKESDRGA +APLM A +IDI LLSSS
Sbjct: 11  NAVVSNLPAMASTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSS 70

Query: 275 SNSGPELQKFRHGLQSWGCFLAKNHGMSSELLEEVRQVMKEFFALPMEEKLKCSREEDDT 334
           S SGPEL+K RHGLQSWGCF A NHGM+SE L+EVRQ+ K+FF L MEEKLK  +EE + 
Sbjct: 71  SKSGPELEKLRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEM 130

Query: 335 EGYGNAMILSNDQVLDWTDRLNLTLYPEESRRLKYWPTTPERFREVIYEYTENVRALCEK 394
           EGYGN MILSN Q+LDWTDRL LT+YP +SRR KYWPT P+RFREV+ EYT NV+ + EK
Sbjct: 131 EGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNPQRFREVVGEYTTNVKLISEK 190

Query: 395 ILKAMARSLDLDENSFVNEFVDGYKL-AYFNFYPPCPNPDLVLGLKPHSDGSSITILLQD 454
           ILKAMARSLDLDE+SF+N++ +  KL A FNFYP C NPDLVLG+KPH+DGS+ITILLQD
Sbjct: 191 ILKAMARSLDLDESSFLNQYGEQVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQD 250

Query: 455 KEVEGLQILKGNEWFNAPIVPDALLVLVGDQGEITSNGIFKSVVHRVLTNTERERISLAT 514
           KEVEGLQ +K NEW+NAPIVPDALLV VGDQ EITSNGIFKS VHRVLTN+ERERISLA 
Sbjct: 251 KEVEGLQFMKDNEWYNAPIVPDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAV 310

Query: 515 LYLPHSEKEIEPLEKLINETRPRLYKTVKNFASLYYQYYQKGQRPIEAARI 565
            YLP SEKEIEPLE+LINE++PRLYK+VKNF  LY++YYQ+GQRP+EAARI
Sbjct: 311 FYLPDSEKEIEPLEELINESQPRLYKSVKNFVGLYFEYYQQGQRPMEAARI 361

BLAST of Lag0034266 vs. NCBI nr
Match: XP_022987763.1 (probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 [Cucurbita maxima])

HSP 1 Score: 532.7 bits (1371), Expect = 3.8e-147
Identity = 263/342 (76.90%), Postives = 295/342 (86.26%), Query Frame = 0

Query: 224 MAGINPSGAVQDVASKGEVPERYIHKESDRGALDAPLMEASIIDIDLLSSSSNSGPELQK 283
           MAG NPSG VQDVASKGEVPERYIH ESDRGALDAPLM A +ID+ LLSS S SGPEL+K
Sbjct: 1   MAGTNPSGTVQDVASKGEVPERYIHIESDRGALDAPLMAAPVIDMALLSSPSKSGPELEK 60

Query: 284 FRHGLQSWGCFLAKNHGMSSELLEEVRQVMKEFFALPMEEKLKCSREEDDTEGYGNAMIL 343
            RHGLQSWGCF   NHGMS+E L+EVR++ K+FF LPMEEK K SREE++ EGYGN MIL
Sbjct: 61  LRHGLQSWGCFQVVNHGMSAEFLDEVRRLAKQFFDLPMEEKSKYSREENEIEGYGNDMIL 120

Query: 344 SNDQVLDWTDRLNLTLYPEESRRLKYWPTTPERFREVIYEYTENVRALCEKILKAMARSL 403
           SN Q+LDWTDRL LT+YP+ES R KYWPT PERFREV++EYT NV+ L EKILKAMA SL
Sbjct: 121 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMAISL 180

Query: 404 DLDENSFVNEFVDGYKL-AYFNFYPPCPNPDLVLGLKPHSDGSSITILLQDKEVEGLQIL 463
           DL+E+SF+ ++ +  KL A FNFYP C NPDLVLG+KPH+DGS+ITILLQD+EVEGLQ L
Sbjct: 181 DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL 240

Query: 464 KGNEWFNAPIVPDALLVLVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKE 523
            GNEWFNAPIVP ALLV VGDQ EITSNGIFKS VHRVLTN+ERERISLA  YLP SEKE
Sbjct: 241 NGNEWFNAPIVPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 300

Query: 524 IEPLEKLINETRPRLYKTVKNFASLYYQYYQKGQRPIEAARI 565
           IEPLEKLI+ETRPRLYK+VKNF  LY+QYYQ+GQRP+EAARI
Sbjct: 301 IEPLEKLIDETRPRLYKSVKNFVGLYFQYYQQGQRPMEAARI 342

BLAST of Lag0034266 vs. NCBI nr
Match: XP_023516521.1 (protein SRG1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 530.0 bits (1364), Expect = 2.5e-146
Identity = 262/342 (76.61%), Postives = 294/342 (85.96%), Query Frame = 0

Query: 224 MAGINPSGAVQDVASKGEVPERYIHKESDRGALDAPLMEASIIDIDLLSSSSNSGPELQK 283
           MAG NPSG VQ VASKGEVPERYIHKESDRGA DAPLM A +ID+ LLSSSS SGPEL+K
Sbjct: 1   MAGTNPSGTVQGVASKGEVPERYIHKESDRGARDAPLMAAPVIDMALLSSSSKSGPELEK 60

Query: 284 FRHGLQSWGCFLAKNHGMSSELLEEVRQVMKEFFALPMEEKLKCSREEDDTEGYGNAMIL 343
            RHGLQSWGCF   NHGMS+E L+EVR++ K+FF LPMEEK K SREE++ EGYGN MIL
Sbjct: 61  LRHGLQSWGCFQVVNHGMSAEFLDEVRRLAKQFFDLPMEEKSKYSREENEIEGYGNDMIL 120

Query: 344 SNDQVLDWTDRLNLTLYPEESRRLKYWPTTPERFREVIYEYTENVRALCEKILKAMARSL 403
           SN Q+LDWTDRL LT+YP+ES R KYWPT PERFREV++EYT NV+ L EKILKAMA SL
Sbjct: 121 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMAISL 180

Query: 404 DLDENSFVNEFVDGYKL-AYFNFYPPCPNPDLVLGLKPHSDGSSITILLQDKEVEGLQIL 463
           DL+E+SF+ ++ +  KL A FNFYP C NPDLVLG+KPH+DGS+ITILLQD+EVEGLQ L
Sbjct: 181 DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL 240

Query: 464 KGNEWFNAPIVPDALLVLVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKE 523
            GNEWFNAPIVP ALLV VGDQ EITSNGIFKS VHRVLTN+ERERISLA  YLP  +KE
Sbjct: 241 NGNEWFNAPIVPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDWKKE 300

Query: 524 IEPLEKLINETRPRLYKTVKNFASLYYQYYQKGQRPIEAARI 565
           IEPLEKLI+ETRPRLYKTVKNF  LY+QYYQ+GQRP+EAARI
Sbjct: 301 IEPLEKLIDETRPRLYKTVKNFVGLYFQYYQQGQRPMEAARI 342

BLAST of Lag0034266 vs. ExPASy Swiss-Prot
Match: O80449 (Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 1.9e-61
Identity = 134/342 (39.18%), Postives = 192/342 (56.14%), Query Frame = 0

Query: 229 PSGAVQDVASKG--EVPERYIHKESDRGALDAPLMEASIIDIDLLSSSSNSG-PE-LQKF 288
           P  +VQ ++  G   VP RY+     R   +    +A  I+I +L  +   G PE L+  
Sbjct: 8   PIVSVQSLSQTGVPTVPNRYVKPAHQRPVFNTTQSDAG-IEIPVLDMNDVWGKPEGLRLV 67

Query: 289 RHGLQSWGCFLAKNHGMSSELLEEVRQVMKEFFALPMEEKLKCSREEDDTEGYGNAMILS 348
           R   + WG F   NHG++  L+E VR   +EFF LP+EEK K +   D  EGYG+ + + 
Sbjct: 68  RSACEEWGFFQMVNHGVTHSLMERVRGAWREFFELPLEEKRKYANSPDTYEGYGSRLGVV 127

Query: 349 NDQVLDWTDRLNLTLYPEESRRLKYWPTTPERFREVIYEYTENVRALCEKILKAMARSLD 408
            D  LDW+D   L   P   R    WP+ P + RE+I +Y E VR LCE++ + ++ SL 
Sbjct: 128 KDAKLDWSDYFFLNYLPSSIRNPSKWPSQPPKIRELIEKYGEEVRKLCERLTETLSESLG 187

Query: 409 LDENSFVNEFVDGYKLA---YFNFYPPCPNPDLVLGLKPHSDGSSITILLQDKEVEGLQI 468
           L  N  +     G K+      NFYP CP P L LGL  HSD   ITILL D++V GLQ+
Sbjct: 188 LKPNKLMQALGGGDKVGASLRTNFYPKCPQPQLTLGLSSHSDPGGITILLPDEKVAGLQV 247

Query: 469 LKGNEWFNAPIVPDALLVLVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEK 528
            +G+ W     VP+AL+V +GDQ +I SNGI+KSV H+V+ N+  ER+SLA  Y P S+ 
Sbjct: 248 RRGDGWVTIKSVPNALIVNIGDQLQILSNGIYKSVEHQVIVNSGMERVSLAFFYNPRSDI 307

Query: 529 EIEPLEKLINETRPRLYKTVK--NFASLYYQYYQKGQRPIEA 562
            + P+E+L+   RP LYK ++   + SL  Q    G+  +++
Sbjct: 308 PVGPIEELVTANRPALYKPIRFDEYRSLIRQKGPCGKNQVDS 348

BLAST of Lag0034266 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 5.8e-58
Identity = 118/330 (35.76%), Postives = 188/330 (56.97%), Query Frame = 0

Query: 242 VPERYIHKESDRGALDAPL---MEASIIDIDLLSSSSNSGPELQKFRHGLQSWGCFLAKN 301
           VP RY+  + D+  +D      +E  IID+  L SS+    E++K     + WG F   N
Sbjct: 29  VPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDSEVEKLDFACKEWGFFQLVN 88

Query: 302 HGMSSELLEEVRQVMKEFFALPMEEKLKCSREEDDTEGYGNAMILSNDQVLDWTDRLNLT 361
           HG+ S  L++V+  +++FF LPMEEK K  +  D+ EG+G A ++S DQ LDW D    T
Sbjct: 89  HGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQAFVVSEDQKLDWADLFFHT 148

Query: 362 LYPEESRRLKYWPTTPERFREVIYEYTENVRALCEKILKAMARSLDLDENSFVNEF--VD 421
           + P E R+   +P  P  FR+ +  Y+  V+++ + ++  MAR+L++        F  VD
Sbjct: 149 VQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKMARALEIKPEELEKLFDDVD 208

Query: 422 GYKLAYFNFYPPCPNPDLVLGLKPHSDGSSITILLQDKEVEGLQILKGNEWFNAPIVPDA 481
             +    N+YPPCP PD V+GL PHSD   +T+L+Q  +VEGLQI K  +W     +P+A
Sbjct: 209 SVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDVEGLQIKKDGKWVPVKPLPNA 268

Query: 482 LLVLVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKEIEPLEKLINETRPR 541
            +V +GD  EI +NG ++S+ HR + N+E+ER+S+AT +     KE+ P + L+   +  
Sbjct: 269 FIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHNVGMYKEVGPAKSLVERQKVA 328

Query: 542 LYK--TVKNFASLYYQYYQKGQRPIEAARI 565
            +K  T+K +    +     G+  ++A RI
Sbjct: 329 RFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

BLAST of Lag0034266 vs. ExPASy Swiss-Prot
Match: D4N502 (Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 8.7e-54
Identity = 123/317 (38.80%), Postives = 178/317 (56.15%), Query Frame = 0

Query: 232 AVQDVA--SKGEVPERY-IHKESDRGALDAPLME---ASIIDI-DLLSSSSNSGP-ELQK 291
           +VQ++A  +  E+P RY    ES    + A + +     +ID+ +LLS     G  EL K
Sbjct: 17  SVQELAKLTLAEIPSRYTCTGESPLNNIGASVTDDETVPVIDLQNLLSPEPVVGKLELDK 76

Query: 292 FRHGLQSWGCFLAKNHGMSSELLEEVRQVMKEFFALPMEEKLKCSREEDDTEGYGNAMIL 351
                + WG F   NHG+ + L++ ++  +K FF LPM EK K  +++ D EG+G   I 
Sbjct: 77  LHSACKEWGFFQLVNHGVDALLMDNIKSEIKGFFNLPMNEKTKYGQQDGDFEGFGQPYIE 136

Query: 352 SNDQVLDWTDRLNLTLYPEESRRLKYWPTTPERFREVIYEYTENVRALCEKILKAMARSL 411
           S DQ LDWT+  ++   P   R+   +P  P  FRE +  Y   ++ L   + + + +SL
Sbjct: 137 SEDQRLDWTEVFSMLSLPLHLRKPHLFPELPLPFRETLESYLSKMKKLSTVVFEMLEKSL 196

Query: 412 DLDE-NSFVNEFVDGYKLAYFNFYPPCPNPDLVLGLKPHSDGSSITILLQDKEVEGLQIL 471
            L E     + F DG +    N+YPPCP P+LVLGL  HSD S +TILLQ  EVEGLQI 
Sbjct: 197 QLVEIKGMTDLFEDGLQTMRMNYYPPCPRPELVLGLTSHSDFSGLTILLQLNEVEGLQIR 256

Query: 472 KGNEWFNAPIVPDALLVLVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKE 531
           K   W +   +PDA +V VGD  EI +NGI++SV HR + N+ +ER+S+AT +    E E
Sbjct: 257 KEERWISIKPLPDAFIVNVGDILEIMTNGIYRSVEHRAVVNSTKERLSIATFHDSKLESE 316

Query: 532 IEPLEKLINETRPRLYK 540
           I P+  L+    P L+K
Sbjct: 317 IGPISSLVTPETPALFK 333

BLAST of Lag0034266 vs. ExPASy Swiss-Prot
Match: Q94LP4 (2-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD11 PE=1 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 6.3e-52
Identity = 112/331 (33.84%), Postives = 181/331 (54.68%), Query Frame = 0

Query: 242 VPERYIHKESDRGALDAPL---MEASIIDIDLLSSSSNSGPELQKFRHGLQSWGCFLAKN 301
           +PERYI  E+    +       M   IID+  L    +S  E  K R   Q WG FL  N
Sbjct: 29  IPERYIRPEASSEEVINNYQGDMAIPIIDLKKLLCPQSSEEECVKLRSACQYWGFFLLIN 88

Query: 302 HGMSSELLEEVRQVMKEFFALPMEEKLKCSREEDDTEGYGNAMILSNDQVLDWTDRLNLT 361
           HG+  E++  +++ + +FF+ P++ K + ++  +  EGYG + + S DQ LDW D L L 
Sbjct: 89  HGVPDEVIANLKRDIVDFFSQPLDTKKEYTQLPNSLEGYGQSFVFSEDQKLDWADMLYLH 148

Query: 362 LYPEESRRLKYWPTTPERFREVIYEYTENVRALCEKILKAMARSLDLDENSFVNEFVD-- 421
           ++P +SR L++WPT+P  FR+ I  Y+   ++L   + + MA+++     S ++ F +  
Sbjct: 149 VHPSDSRDLRFWPTSPASFRQSIDAYSSETKSLALCLFEFMAKAVGAKPESLLDLFEEQP 208

Query: 422 -GYKLAYFNFYPPCPNPDLVLGLKPHSDGSSITILLQDKEVEGLQILKGNEWFNAPIVPD 481
            G ++AY   YPPC   D V+GL PHSD   +T+LL+   V+GLQI K  +WF+      
Sbjct: 209 RGLRMAY---YPPCRQADKVMGLSPHSDAGGLTLLLEINNVQGLQIKKDGKWFSIDAPNG 268

Query: 482 ALLVLVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKEIEPLEKLINETRP 541
           AL+  +GD  EI SNG F+SV HR + N  +ERIS A  + P     I PL + + + + 
Sbjct: 269 ALIANIGDTLEILSNGKFRSVEHRAVINPNKERISAALFHYPSENMVISPLPEFVKDGKV 328

Query: 542 RLYKTVK--NFASLYYQYYQKGQRPIEAARI 565
           + Y+++   +F    +     G+  +E  ++
Sbjct: 329 K-YRSISYLDFMKQIFTQQLDGKNRVEVLKL 355

BLAST of Lag0034266 vs. ExPASy Swiss-Prot
Match: D4N501 (Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=3469 GN=DIOX2 PE=2 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 2.4e-51
Identity = 116/335 (34.63%), Postives = 187/335 (55.82%), Query Frame = 0

Query: 232 AVQDVA--SKGEVPERYI-HKESDRGALDAPLME----ASIIDIDLLSSSS--NSGPELQ 291
           +VQ++A  +  E+P RYI   E+ +  + A +++      +IDI+ L SS       EL 
Sbjct: 17  SVQELAKLTLAEIPSRYICTVENLQLPVGASVIDDHETVPVIDIENLISSEPVTEKLELD 76

Query: 292 KFRHGLQSWGCFLAKNHGMSSELLEEVRQVMKEFFALPMEEKLKCSREEDDTEGYGNAMI 351
           +     + WG F   NHG+ + L++ V+  ++ FF L M EK+K  +++ D EG+G A +
Sbjct: 77  RLHSACKEWGFFQVVNHGVDTSLVDNVKSDIQGFFNLSMNEKIKYGQKDGDVEGFGQAFV 136

Query: 352 LSNDQVLDWTDRLNLTLYPEESRRLKYWPTTPERFREVIYEYTENVRALCEKILKAMARS 411
            S DQ LDW D   +   P   R+   +   P   RE I  Y+  ++ L   + + M ++
Sbjct: 137 ASEDQTLDWADIFMILTLPLHLRKPHLFSKLPLPLRETIESYSSEMKKLSMVLFEKMEKA 196

Query: 412 LDLD--ENSFVNE-FVDGYKLAYFNFYPPCPNPDLVLGLKPHSDGSSITILLQDKEVEGL 471
           L +   E   ++E F D  ++   N+YPPCP P+L +GL PHSD   +TILLQ  EVEGL
Sbjct: 197 LQVQAVEIKEISEVFKDMTQVMRMNYYPPCPQPELAIGLTPHSDFGGLTILLQLNEVEGL 256

Query: 472 QILKGNEWFNAPIVPDALLVLVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHS 531
           QI     W +   +P+A +V VGD  EI +NG+++SV HR + N+ +ER+S+AT + P+ 
Sbjct: 257 QIKNEGRWISVKPLPNAFVVNVGDVLEIMTNGMYRSVDHRAVVNSTKERLSIATFHDPNL 316

Query: 532 EKEIEPLEKLINETRPRLYKTVKNFASLYYQYYQK 555
           E EI P+  LI    P L+++   +  L  +++ +
Sbjct: 317 ESEIGPISSLITPNTPALFRSGSTYGELVEEFHSR 351

BLAST of Lag0034266 vs. ExPASy TrEMBL
Match: A0A6J1BYU1 (uncharacterized protein LOC111006992 OS=Momordica charantia OX=3673 GN=LOC111006992 PE=4 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 1.5e-149
Identity = 268/342 (78.36%), Postives = 296/342 (86.55%), Query Frame = 0

Query: 224 MAGINPSGAVQDVASKGEVPERYIHKESDRGALDAPLMEASIIDIDLLSSSSNSGPELQK 283
           MAG NP+G+VQDVASKGEVPERYIHKE DRGALDAPLMEA +IDI LLSS SN+GPEL+K
Sbjct: 1   MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK 60

Query: 284 FRHGLQSWGCFLAKNHGMSSELLEEVRQVMKEFFALPMEEKLKCSREEDDTEGYGNAMIL 343
            RHGL SWGCF A NHGMS E LEEVRQV K FFALPME+KLK SREED  EGYGN MI 
Sbjct: 61  LRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIF 120

Query: 344 SNDQVLDWTDRLNLTLYPEESRRLKYWPTTPERFREVIYEYTENVRALCEKILKAMARSL 403
           SN Q+LDWTDRL LT+ PEESRR KYWPT PERFREV++EYT NV+ L EKILKAMARSL
Sbjct: 121 SNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSL 180

Query: 404 DLDENSFVNEFVDGYKL-AYFNFYPPCPNPDLVLGLKPHSDGSSITILLQDKEVEGLQIL 463
           DLDENSF+N++    +L A FNFY  C NP+LVLG+KPH+DGS+ITILLQDKEVEGLQ L
Sbjct: 181 DLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL 240

Query: 464 KGNEWFNAPIVPDALLVLVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKE 523
           KGNEW+NAPI+PDALLV VGDQGEITSNGIFKS VHRVLTN+ERERISLA  YLP  +KE
Sbjct: 241 KGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKE 300

Query: 524 IEPLEKLINETRPRLYKTVKNFASLYYQYYQKGQRPIEAARI 565
           IEPLEKLINET PRLY+TVKNF  L++QYYQ+GQRP EAA+I
Sbjct: 301 IEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKI 342

BLAST of Lag0034266 vs. ExPASy TrEMBL
Match: A0A6J1H9T6 (protein SRG1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461440 PE=3 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 1.7e-148
Identity = 265/351 (75.50%), Postives = 299/351 (85.19%), Query Frame = 0

Query: 215 SATISATSAMAGINPSGAVQDVASKGEVPERYIHKESDRGALDAPLMEASIIDIDLLSSS 274
           ++  SA  AMAG NPSG VQDVASKGEVPERYIHKESDRGA DAPLM A +ID+ LLSSS
Sbjct: 11  NSVASAPPAMAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAPVIDMALLSSS 70

Query: 275 SNSGPELQKFRHGLQSWGCFLAKNHGMSSELLEEVRQVMKEFFALPMEEKLKCSREEDDT 334
           S SGPEL+K RHGLQSWGCF   NHGMS+E L+E+R++ K+FF LPMEEK K SREED+ 
Sbjct: 71  SESGPELEKLRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEI 130

Query: 335 EGYGNAMILSNDQVLDWTDRLNLTLYPEESRRLKYWPTTPERFREVIYEYTENVRALCEK 394
           EGYGN MILSN Q+LDWTDRL LT+YP+ES R KYWPT PERFR V++EYT NV+ L EK
Sbjct: 131 EGYGNDMILSNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEYTANVKLLSEK 190

Query: 395 ILKAMARSLDLDENSFVNEFVDGYKL-AYFNFYPPCPNPDLVLGLKPHSDGSSITILLQD 454
           ILKAMA SLDL+E+SF+ ++ +  KL A FNFYP C NPDLVLG+KPH+DGS+ITILLQD
Sbjct: 191 ILKAMAISLDLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQD 250

Query: 455 KEVEGLQILKGNEWFNAPIVPDALLVLVGDQGEITSNGIFKSVVHRVLTNTERERISLAT 514
           +EVEGLQ L GNEWFNAPIVP ALL+ VGDQ EITSNGIFKS VHRVLTN+ERERISLA 
Sbjct: 251 EEVEGLQFLNGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTNSERERISLAV 310

Query: 515 LYLPHSEKEIEPLEKLINETRPRLYKTVKNFASLYYQYYQKGQRPIEAARI 565
            YLP  EKEIEPLEKLI+ETRPRLYKTVKNF  LY+QYYQ+GQRP+EAARI
Sbjct: 311 FYLPDWEKEIEPLEKLIDETRPRLYKTVKNFVGLYFQYYQQGQRPMEAARI 361

BLAST of Lag0034266 vs. ExPASy TrEMBL
Match: A0A5A7U2M0 (Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00660 PE=3 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 8.2e-148
Identity = 264/351 (75.21%), Postives = 301/351 (85.75%), Query Frame = 0

Query: 215 SATISATSAMAGINPSGAVQDVASKGEVPERYIHKESDRGALDAPLMEASIIDIDLLSSS 274
           +A +S   AMA  NPSG VQDVASKGEVPERYIHKESDRGA +APLM A +IDI LLSSS
Sbjct: 11  NAVVSNLPAMASTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSS 70

Query: 275 SNSGPELQKFRHGLQSWGCFLAKNHGMSSELLEEVRQVMKEFFALPMEEKLKCSREEDDT 334
           S SGPEL+K RHGLQSWGCF A NHGM+SE L+EVRQ+ K+FF L MEEKLK  +EE + 
Sbjct: 71  SKSGPELEKLRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEM 130

Query: 335 EGYGNAMILSNDQVLDWTDRLNLTLYPEESRRLKYWPTTPERFREVIYEYTENVRALCEK 394
           EGYGN MILSN Q+LDWTDRL LT+YP +SRR KYWPT P+RFREV+ EYT NV+ + EK
Sbjct: 131 EGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNPQRFREVVGEYTTNVKLISEK 190

Query: 395 ILKAMARSLDLDENSFVNEFVDGYKL-AYFNFYPPCPNPDLVLGLKPHSDGSSITILLQD 454
           ILKAMARSLDLDE+SF+N++ +  KL A FNFYP C NPDLVLG+KPH+DGS+ITILLQD
Sbjct: 191 ILKAMARSLDLDESSFLNQYGEQVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQD 250

Query: 455 KEVEGLQILKGNEWFNAPIVPDALLVLVGDQGEITSNGIFKSVVHRVLTNTERERISLAT 514
           KEVEGLQ +K NEW+NAPIVPDALLV VGDQ EITSNGIFKS VHRVLTN+ERERISLA 
Sbjct: 251 KEVEGLQFMKDNEWYNAPIVPDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAV 310

Query: 515 LYLPHSEKEIEPLEKLINETRPRLYKTVKNFASLYYQYYQKGQRPIEAARI 565
            YLP SEKEIEPLE+LINE++PRLYK+VKNF  LY++YYQ+GQRP+EAARI
Sbjct: 311 FYLPDSEKEIEPLEELINESQPRLYKSVKNFVGLYFEYYQQGQRPMEAARI 361

BLAST of Lag0034266 vs. ExPASy TrEMBL
Match: A0A6J1JKD6 (probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485215 PE=3 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 1.8e-147
Identity = 263/342 (76.90%), Postives = 295/342 (86.26%), Query Frame = 0

Query: 224 MAGINPSGAVQDVASKGEVPERYIHKESDRGALDAPLMEASIIDIDLLSSSSNSGPELQK 283
           MAG NPSG VQDVASKGEVPERYIH ESDRGALDAPLM A +ID+ LLSS S SGPEL+K
Sbjct: 1   MAGTNPSGTVQDVASKGEVPERYIHIESDRGALDAPLMAAPVIDMALLSSPSKSGPELEK 60

Query: 284 FRHGLQSWGCFLAKNHGMSSELLEEVRQVMKEFFALPMEEKLKCSREEDDTEGYGNAMIL 343
            RHGLQSWGCF   NHGMS+E L+EVR++ K+FF LPMEEK K SREE++ EGYGN MIL
Sbjct: 61  LRHGLQSWGCFQVVNHGMSAEFLDEVRRLAKQFFDLPMEEKSKYSREENEIEGYGNDMIL 120

Query: 344 SNDQVLDWTDRLNLTLYPEESRRLKYWPTTPERFREVIYEYTENVRALCEKILKAMARSL 403
           SN Q+LDWTDRL LT+YP+ES R KYWPT PERFREV++EYT NV+ L EKILKAMA SL
Sbjct: 121 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMAISL 180

Query: 404 DLDENSFVNEFVDGYKL-AYFNFYPPCPNPDLVLGLKPHSDGSSITILLQDKEVEGLQIL 463
           DL+E+SF+ ++ +  KL A FNFYP C NPDLVLG+KPH+DGS+ITILLQD+EVEGLQ L
Sbjct: 181 DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL 240

Query: 464 KGNEWFNAPIVPDALLVLVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKE 523
            GNEWFNAPIVP ALLV VGDQ EITSNGIFKS VHRVLTN+ERERISLA  YLP SEKE
Sbjct: 241 NGNEWFNAPIVPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 300

Query: 524 IEPLEKLINETRPRLYKTVKNFASLYYQYYQKGQRPIEAARI 565
           IEPLEKLI+ETRPRLYK+VKNF  LY+QYYQ+GQRP+EAARI
Sbjct: 301 IEPLEKLIDETRPRLYKSVKNFVGLYFQYYQQGQRPMEAARI 342

BLAST of Lag0034266 vs. ExPASy TrEMBL
Match: A0A5D3CIZ6 (Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold367G00230 PE=3 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 7.7e-146
Identity = 261/351 (74.36%), Postives = 299/351 (85.19%), Query Frame = 0

Query: 215 SATISATSAMAGINPSGAVQDVASKGEVPERYIHKESDRGALDAPLMEASIIDIDLLSSS 274
           +A +S   AMA  NPSG VQDVASKGEVPERYIHKESDRGA +APLM A +IDI LLSSS
Sbjct: 11  NAVVSNLPAMASTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSS 70

Query: 275 SNSGPELQKFRHGLQSWGCFLAKNHGMSSELLEEVRQVMKEFFALPMEEKLKCSREEDDT 334
           S SGPEL+K RHGLQSWGCF A NHGM+SE L+EVRQ+ K+FF L MEEKLK  +EE + 
Sbjct: 71  SKSGPELEKLRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEM 130

Query: 335 EGYGNAMILSNDQVLDWTDRLNLTLYPEESRRLKYWPTTPERFREVIYEYTENVRALCEK 394
           EGYGN MILSN Q+LDWTDRL LT+YP +SRR KYWP  P+RFREV+ EYT NV+ + EK
Sbjct: 131 EGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPINPQRFREVVGEYTTNVKLISEK 190

Query: 395 ILKAMARSLDLDENSFVNEFVDGYKL-AYFNFYPPCPNPDLVLGLKPHSDGSSITILLQD 454
           ILKAMARSLDLDE+SF+N++ +  KL A FNFYP C NPDLVLG+KPH+DGS+ITILLQD
Sbjct: 191 ILKAMARSLDLDESSFLNQYGEQVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQD 250

Query: 455 KEVEGLQILKGNEWFNAPIVPDALLVLVGDQGEITSNGIFKSVVHRVLTNTERERISLAT 514
           KEVEGLQ +K NEW+NAPIV DALLV VGDQ EITSNGIFKS VHRVLTN+ERERISLA 
Sbjct: 251 KEVEGLQFMKDNEWYNAPIVTDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAV 310

Query: 515 LYLPHSEKEIEPLEKLINETRPRLYKTVKNFASLYYQYYQKGQRPIEAARI 565
            YLP SEKEIEPLE+LINE++PRLYK+VKNF  LY++YYQ+G+RP+EAARI
Sbjct: 311 FYLPDSEKEIEPLEELINESQPRLYKSVKNFVGLYFEYYQQGERPMEAARI 361

BLAST of Lag0034266 vs. TAIR 10
Match: AT5G20400.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 371.7 bits (953), Expect = 1.0e-102
Identity = 182/340 (53.53%), Postives = 245/340 (72.06%), Query Frame = 0

Query: 233 VQDVASKGE-VPERYIHKESDRGALD-----APLMEASIIDIDLLSSSSNSG-PELQKFR 292
           VQ+V + GE +PERY+H  +  G +       P M+   ID++LL SSS +G  EL K  
Sbjct: 9   VQEVVAAGEGLPERYLHAPTGDGEVQPLNAAVPEMDIPAIDLNLLLSSSEAGQQELSKLH 68

Query: 293 HGLQSWGCFLAKNHGMSSELLEEVRQVMKEFFALPMEEKLKCSREEDDTEGYGNAMILSN 352
             L +WG     NHG++   L+++ ++ KEFFALP EEK KC+RE D  +GYGN MIL +
Sbjct: 69  SALSTWGVVQVMNHGITKAFLDKIYKLTKEFFALPTEEKQKCAREIDSIQGYGNDMILWD 128

Query: 353 DQVLDWTDRLNLTLYPEESRRLKYWPTTPERFREVIYEYTENVRALCEKILKAMARSLDL 412
           DQVLDW DRL +T YPE+ R+L +WP  P  FRE ++EYT   R + E+  KAMARSL+L
Sbjct: 129 DQVLDWIDRLYITTYPEDQRQLNFWPEVPLGFRETLHEYTMKQRIVIEQFFKAMARSLEL 188

Query: 413 DENSFVNEFVDGYKL-AYFNFYPPCPNPDLVLGLKPHSDGSSITILLQDKEVEGLQILKG 472
           +ENSF++ + +   L   FN YPPCP+PD V+G+KPH+DGS+IT+LL DK+V GLQ  K 
Sbjct: 189 EENSFLDMYGESATLDTRFNMYPPCPSPDKVIGVKPHADGSAITLLLPDKDVGGLQFQKD 248

Query: 473 NEWFNAPIVPDALLVLVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKEIE 532
            +W+ APIVPD +L+ VGDQ EI SNGI+KS VHRV+TN E+ERIS+AT  +P ++KEI+
Sbjct: 249 GKWYKAPIVPDTILINVGDQMEIMSNGIYKSPVHRVVTNREKERISVATFCIPGADKEIQ 308

Query: 533 PLEKLINETRPRLYKTVKNFASLYYQYYQKGQRPIEAARI 565
           P+ +L++E RPRLYKTVK +  LY++YYQ+G+RPIEAA I
Sbjct: 309 PVNELVSEARPRLYKTVKKYVELYFKYYQQGRRPIEAALI 348

BLAST of Lag0034266 vs. TAIR 10
Match: AT1G49390.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 359.8 bits (922), Expect = 4.1e-99
Identity = 178/340 (52.35%), Postives = 238/340 (70.00%), Query Frame = 0

Query: 233 VQDVASKGE-VPERYIHKESDRGALD-----APLMEASIIDIDLLSSSSNSG-PELQKFR 292
           VQ+V + G+ +PERY+H  +  G         P M+   ID+ LL SSS  G  E++K  
Sbjct: 9   VQEVVAAGQGLPERYLHAPTGEGESQPLNGAVPEMDIPAIDLSLLFSSSVDGQEEMKKLH 68

Query: 293 HGLQSWGCFLAKNHGMSSELLEEVRQVMKEFFALPMEEKLKCSREEDDTEGYGNAMILSN 352
             L +WG     NHG++   L+++ ++ K+FFALP EEK KC+RE  + +GYGN MILS+
Sbjct: 69  SALSTWGVVQVMNHGITEAFLDKIYKLTKQFFALPTEEKHKCARETGNIQGYGNDMILSD 128

Query: 353 DQVLDWTDRLNLTLYPEESRRLKYWPTTPERFREVIYEYTENVRALCEKILKAMARSLDL 412
           +QVLDW DRL LT YPE+ R+LK+WP  P  F E + EYT   R L EK  KAMARSL+L
Sbjct: 129 NQVLDWIDRLFLTTYPEDKRQLKFWPQVPVGFSETLDEYTMKQRVLIEKFFKAMARSLEL 188

Query: 413 DENSFVNEFVDGYKL-AYFNFYPPCPNPDLVLGLKPHSDGSSITILLQDKEVEGLQILKG 472
           +EN F+  + +   + + FNF+PPCP PD V+G+KPH+DGS+IT+LL DK+VEGLQ LK 
Sbjct: 189 EENCFLEMYGENAVMNSRFNFFPPCPRPDKVIGIKPHADGSAITLLLPDKDVEGLQFLKD 248

Query: 473 NEWFNAPIVPDALLVLVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKEIE 532
            +W+ APIVPD +L+ +GDQ EI SNGI+KS VHRV+TN E+ERIS+AT  +P  +KEI 
Sbjct: 249 GKWYKAPIVPDTILITLGDQMEIMSNGIYKSPVHRVVTNREKERISVATFCVPGLDKEIH 308

Query: 533 PLEKLINETRPRLYKTVKNFASLYYQYYQKGQRPIEAARI 565
           P + L+ E RPRLYKTV  +  L+Y+YYQ+G+R IEAA I
Sbjct: 309 PADGLVTEARPRLYKTVTKYVDLHYKYYQQGRRTIEAALI 348

BLAST of Lag0034266 vs. TAIR 10
Match: AT5G54000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 337.4 bits (864), Expect = 2.2e-92
Identity = 170/341 (49.85%), Postives = 229/341 (67.16%), Query Frame = 0

Query: 233 VQDVASKGE-VPERYIHKESDRGALDAPL------MEASIIDIDLLSSSSNSG-PELQKF 292
           VQ+V + GE +PERY++  +  G  D P       M+ SIID++LL SSS+ G  EL K 
Sbjct: 9   VQEVVAAGEKLPERYLYTPTGDGEGDQPFNGLLPEMKISIIDLNLLFSSSDDGREELSKL 68

Query: 293 RHGLQSWGCFLAKNHGMSSELLEEVRQVMKEFFALPMEEKLKCSREEDDTEGYGNAMILS 352
              + +WG     NHG+S  LL+++ ++ K+FF LP +EK K +RE    +G+GN MILS
Sbjct: 69  HSAISTWGVVQVMNHGISEALLDKIHELTKQFFVLPTKEKQKYAREISSFQGFGNDMILS 128

Query: 353 NDQVLDWTDRLNLTLYPEESRRLKYWPTTPERFREVIYEYTENVRALCEKILKAMARSLD 412
           +DQVLDW DRL L  YPE+ R+LK+WP  P  FRE ++EYT   + + EK  KA+ARSL+
Sbjct: 129 DDQVLDWVDRLYLITYPEDQRQLKFWPENPSGFRETLHEYTMKQQLVVEKFFKALARSLE 188

Query: 413 LDENSFVNEFVDGYKL-AYFNFYPPCPNPDLVLGLKPHSDGSSITILLQDKEVEGLQILK 472
           L++N F+    +   L   FN YPPCP PD VLGLKPHSDGS+ T++L DK VEGLQ LK
Sbjct: 189 LEDNCFLEMHGENATLETRFNIYPPCPRPDKVLGLKPHSDGSAFTLILPDKNVEGLQFLK 248

Query: 473 GNEWFNAPIVPDALLVLVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKEI 532
             +W+ A I+P  +L+ VGD  E+ SNGI+KS VHRV+ N ++ERI +AT      +KEI
Sbjct: 249 DGKWYKASILPHTILINVGDTMEVMSNGIYKSPVHRVVLNGKKERIYVATFCNADEDKEI 308

Query: 533 EPLEKLINETRPRLYKTVKNFASLYYQYYQKGQRPIEAARI 565
           +PL  L++E RPRLYK VK     ++ YYQ+G+RPIEAA I
Sbjct: 309 QPLNGLVSEARPRLYKAVKKSEKNFFDYYQQGRRPIEAAMI 349

BLAST of Lag0034266 vs. TAIR 10
Match: AT5G20550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 330.9 bits (847), Expect = 2.0e-90
Identity = 172/341 (50.44%), Postives = 235/341 (68.91%), Query Frame = 0

Query: 233 VQDVASKGE-VPERYIHKES--DRGA-LDA--PLMEASIIDIDLLSSSSNSG-PELQKFR 292
           VQ+V + GE +PERY+   +  D G  L+A  P+M+   ID+ LL S S+ G  EL K  
Sbjct: 9   VQEVVAAGEGIPERYLQPPAVDDNGQHLNAAVPVMDIPAIDLSLLLSPSDDGREELSKLH 68

Query: 293 HGLQSWGCFLAKNHGMSSELLEEVRQVMKEFFALPMEEKLKCSREEDDTEGYGNAMILSN 352
             L +WG     NHG++  LL+++ ++ KEF ALP EEK K +RE    +GYGN MIL +
Sbjct: 69  SALSTWGVVQVINHGITKALLDKIYKLTKEFCALPSEEKQKYAREIGSIQGYGNDMILWD 128

Query: 353 DQVLDWTDRLNLTLYPEESRRLKYWPTTPERFREVIYEYTENVRALCEKILKAMARSLDL 412
           DQVLDW DRL +T YPE+ R+LK+WP  P  FRE ++EYT     +  ++ KAMA SL+L
Sbjct: 129 DQVLDWIDRLYITTYPEDQRQLKFWPDVPVGFRETLHEYTMKQHLVFNQVFKAMAISLEL 188

Query: 413 DENSFVNEFVDGYKL-AYFNFYPPCPNPDLVLGLKPHSDGSSITILLQDKEVEGLQILKG 472
           +EN F++   +   +   FN YPPCP PD V+G++PH+D S+ T+LL DK VEGLQ LK 
Sbjct: 189 EENCFLDMCGENATMDTRFNMYPPCPRPDKVIGVRPHADKSAFTLLLPDKNVEGLQFLKD 248

Query: 473 NEWFNAPIV-PDALLVLVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKEI 532
            +W+ AP+V  D +L+ VGDQ EI SNGI+KS VHRV+TNTE+ERIS+AT  +P ++KEI
Sbjct: 249 GKWYKAPVVASDTILINVGDQMEIMSNGIYKSPVHRVVTNTEKERISVATFCIPGADKEI 308

Query: 533 EPLEKLINETRPRLYKTVKNFASLYYQYYQKGQRPIEAARI 565
           +P++ L++E RPRLYK VKN+  L  +YY +GQRPI A+ I
Sbjct: 309 QPVDGLVSEARPRLYKPVKNYVDLLNKYYIQGQRPIAASLI 349

BLAST of Lag0034266 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 258.8 bits (660), Expect = 9.9e-69
Identity = 139/352 (39.49%), Postives = 206/352 (58.52%), Query Frame = 0

Query: 231 GAVQDV-----ASKGEVPERYIHKESDRGALDAPLM------EASIIDIDLLSSSSNSG- 290
           G + DV     +   +VPER+I +E +RG + + L       +  +ID+  LS   N   
Sbjct: 12  GKIDDVQELIKSKPNKVPERFIREEYERGVVVSSLKTHHLHHQIPVIDLSKLSKPDNDDF 71

Query: 291 -PELQKFRHGLQSWGCFLAKNHGMSSELLEEVRQVMKEFFALPMEEKLKCSREEDDTEGY 350
             E+ K     + WG F   NHG+  E++E++ +V  EFF +P+EEK K   E    +GY
Sbjct: 72  FFEILKLSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDMPLEEKKKYPMEPGTVQGY 131

Query: 351 GNAMILSNDQVLDWTDRLNLTLYPEESRRLKYWPTTPERFREVIYEYTENVRALCEKILK 410
           G A I S DQ LDW +   L ++P + R  K WP+ P RF E +  Y++ +R LC+++LK
Sbjct: 132 GQAFIFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARFSESLEGYSKEIRELCKRLLK 191

Query: 411 AMARSLDLDENSFVNEFVDGYKLAYFNFYPPCPNPDLVLGLKPHSDGSSITILLQDK-EV 470
            +A SL L E  F   F +  +    N+YPPC +PDLVLGL PHSDGS++T+L Q K   
Sbjct: 192 YIAISLGLKEERFEEMFGEAVQAVRMNYYPPCSSPDLVLGLSPHSDGSALTVLQQSKNSC 251

Query: 471 EGLQILKGNEWFNAPIVPDALLVLVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYL 530
            GLQILK N W     +P+AL++ +GD  E+ SNG +KSV HR +TN E+ER+++ T Y 
Sbjct: 252 VGLQILKDNTWVPVKPLPNALVINIGDTIEVLSNGKYKSVEHRAVTNREKERLTIVTFYA 311

Query: 531 PHSEKEIEPLEKLI-NETRPRLYKTVKNFASLYYQYYQ---KGQRPIEAARI 565
           P+ E EIEP+ +L+ +ET P  Y++  N     Y Y     +G++ ++ A+I
Sbjct: 312 PNYEVEIEPMSELVDDETNPCKYRSY-NHGDYSYHYVSNKLQGKKSLDFAKI 362

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134811.13.1e-14978.36uncharacterized protein LOC111006992 [Momordica charantia][more]
XP_022960723.13.4e-14875.50protein SRG1-like isoform X1 [Cucurbita moschata][more]
KAA0050082.11.7e-14775.21protein SRG1-like [Cucumis melo var. makuwa][more]
XP_022987763.13.8e-14776.90probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 [Cucurbita maxima][more]
XP_023516521.12.5e-14676.61protein SRG1-like isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
O804491.9e-6139.18Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1[more]
Q392245.8e-5835.76Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1[more]
D4N5028.7e-5438.80Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1[more]
Q94LP46.3e-5233.842-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947... [more]
D4N5012.4e-5134.63Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=34... [more]
Match NameE-valueIdentityDescription
A0A6J1BYU11.5e-14978.36uncharacterized protein LOC111006992 OS=Momordica charantia OX=3673 GN=LOC111006... [more]
A0A6J1H9T61.7e-14875.50protein SRG1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461440 PE=3 ... [more]
A0A5A7U2M08.2e-14875.21Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00... [more]
A0A6J1JKD61.8e-14776.90probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 OS=Cucurbita maxima... [more]
A0A5D3CIZ67.7e-14674.36Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold367G00... [more]
Match NameE-valueIdentityDescription
AT5G20400.11.0e-10253.532-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G49390.14.1e-9952.352-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G54000.12.2e-9249.852-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G20550.12.0e-9050.442-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G21420.19.9e-6939.492-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 232..555
e-value: 3.2E-90
score: 304.8
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 422..515
e-value: 1.1E-22
score: 80.4
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 265..371
e-value: 8.2E-23
score: 81.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 118..210
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 118..141
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 163..185
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 193..210
NoneNo IPR availablePANTHERPTHR47991:SF572OG-FE(II) OXYGENASE FAMILY OXIDOREDUCTASEcoord: 226..560
NoneNo IPR availablePANTHERPTHR47991OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASEcoord: 226..560
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 237..544
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 414..517
score: 12.558978

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0034266.1Lag0034266.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding