Lag0034259 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0034259
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionprotein PHOX1-like
Locationchr3: 5797211 .. 5799543 (+)
RNA-Seq ExpressionLag0034259
SyntenyLag0034259
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGAAGCAAGGTGGTAAGAAGAAGCAGATTGGTGATAAGTTTCGTGAGGCGATTTCGAAACATCGCCAAGGTGGAGATGGTAGTCCAAAAAGTTATGATAAAGACCATGTTATTTTCATTACTATGTCTCAGGTTTTAAAGGAAGAGGGGAATAGATTGTTTCAGTCTAGAGATCTTGAAGGAGCAATATTGAAATATGAAAAGGCCCTCAAATTACTTCCAAGAAATCATATAGATGTATCCTATCTTCGGAGTAACATGGCAGCATGCTATATGCAGATGGGGCTTAGTGAGTATCCCCGGGCGGTTCATGAGTGCAATTTGGCTCTTGAAGTTACGCCGAAGTACAGTAAGGCATTGTTGAAGCGGGCAAGATGTTATGAAGCTTTGCATAGGCTGGACTTGGCTCTAAGAGATGTTAATGCAGTTTTAAACATGGAGCCAAAAAATATCATGGCTTTAGAAATATCAGAGAGACTAACAGAGGCACTTGAGATGAAAGGATCAAAGGATGTCGATGCTGAGATCAAACCGCCTCTTGATTTTGTTGAATTGCCTGCTGCAGTGTTGCCACAAAAGAGACCAAAAGAAAAGAGCCGAAAGAAGAAAAACAACCAAAAGACTAAGGAAACCATTGATGAGAAGAAGGCTGATGAAATTGTGGAGGAAAAGGCTGGTGATGAAATGGTTGAGGAGAAGGCTGACGAAATAATAGTGGAGGAGGAGAAGGCTGACGAAATAATAGTGGAGGAGGAGAAGGCTGAAGAAACAGGGGAGGAGAAGAAGGCCGAGGATAAACTAGTTGTGGAGGAGAAAATCGCAAAGACACAGGAGGAAACACCTATGAAAACTGTGAAATTGGTGTTTGAAGAAGATATTCGATGGGCTCAATTGCCAATTGGTTGCACTCTACTGCAACTAAGGGAGGTTATACGTGATCGTTTCCCAACCTCCACGGCAGTCCTTATCAAGTACAGGGATGAAGAAGGTGATTTGGTAACAATTACTACCAATGAAGAATTGCGATTGGCTGAAACTTCGAACGAGTCACAGGGTTCTGTTAGATTTTATATATTTGAAGTTAGTCCAGAGAAAGACCCATTCTATGAAAGGCTTAACACTAATGAGGTTGCCAAGTGTAAAGTCGAACAAAGTAGCGTGTTTGAGAATGGTCATGTATTAAGAGCAAAAGAAATAAAGATGTCATCTTGCATTGACGACTGGATAATTCAATTTGCTCAGTTATTTATAAACCACGTAGGATTTGAATCTGGCCCATACTTGGATCTCCATGACCTTGGGATGAAGCTTTATACTGAGGCTGTGGAAGAGACAGTAACAAGTGAAGAAGCTCAGGGTCTTTTTGATTTAGCAGCAGAGAAGTTTCACGAGATGGCAGCCTTAGCATTGTTCTACTGGGGAAATGTTATCATGTCAAAGGCGAGGAAGAAGGTCTACTTCACAGATGGTGCTTCAAAAGCACCTTTGTTTGACCAGATCAAAGCTGCATATGATTGGGTCGAAAAAGAATATGCTGAAGCAGGAAGGATATATCAAATGGCGATCGAAATCAAACCAGACTTTTATGAAGGCTATCTAGCACTAGGGCAGCAACAGTTTGAGCAGGCAAAACTTTCTTGGCTTTATGCAGTTAGCAGCAATGTTGATTCGAAAACATTGCCTTGTACTGAGGTTCTGAAACTTTATAACAATGCTGAGGAGAACATGGAAACAGGCATGAAAATGTGGGAAGAATGGGAAGAGCAGCGTACTGGCGAATTCTCTAAATCGAGCAATGTTAAAACCCAGTTGCAGAAGATGGGATTAGATGGGCTGATTAAGGATATATCAGCTGCTGAGGCTGCAGAACAGGCTAAAAATATGAGGTCTCATATAAACCTCTTGTGGGGTACCATGCTCTATGAACGATCAATATTGGAATTTAAGATGGGGCTACCAGTGTGGCATGAATGTCTGGAAGTTGCAGTTGAGAAATTTGAGCTTGCTGGAGCTTCTGCAACAGATATCGCTGTTATGATAAAGAACCACTGCTCAAGCAACAACGCACATGAAGGTACAGTTTAAATATAATTTGTTAATTGTGTAGTATGTGTCCATCCATGTTTAAAAACCCTGTCTTTATTATGTGGAAAATGTTTTTCATATCTCCTCCAATTACAGGTCTCGGGTTCAAAATTGATGAGATAGTACAGGCATGGAACGAGATGTATGAAGCTCGAAAGTTGCTAACTGGAGTTCCATCATTCCGCTTAGAGCCATTATTTAGGCGAAGGGTCTCGAAAATCTACCACGTCTTGGAGGAAGCTTGA

mRNA sequence

ATGGGGAAGCAAGGTGGTAAGAAGAAGCAGATTGGTGATAAGTTTCGTGAGGCGATTTCGAAACATCGCCAAGGTGGAGATGGTAGTCCAAAAAGTTATGATAAAGACCATGTTATTTTCATTACTATGTCTCAGGTTTTAAAGGAAGAGGGGAATAGATTGTTTCAGTCTAGAGATCTTGAAGGAGCAATATTGAAATATGAAAAGGCCCTCAAATTACTTCCAAGAAATCATATAGATGTATCCTATCTTCGGAGTAACATGGCAGCATGCTATATGCAGATGGGGCTTAGTGAGTATCCCCGGGCGGTTCATGAGTGCAATTTGGCTCTTGAAGTTACGCCGAAGTACAGTAAGGCATTGTTGAAGCGGGCAAGATGTTATGAAGCTTTGCATAGGCTGGACTTGGCTCTAAGAGATGTTAATGCAGTTTTAAACATGGAGCCAAAAAATATCATGGCTTTAGAAATATCAGAGAGACTAACAGAGGCACTTGAGATGAAAGGATCAAAGGATGTCGATGCTGAGATCAAACCGCCTCTTGATTTTGTTGAATTGCCTGCTGCAGTGTTGCCACAAAAGAGACCAAAAGAAAAGAGCCGAAAGAAGAAAAACAACCAAAAGACTAAGGAAACCATTGATGAGAAGAAGGCTGATGAAATTGTGGAGGAAAAGGCTGGTGATGAAATGGTTGAGGAGAAGGCTGACGAAATAATAGTGGAGGAGGAGAAGGCTGACGAAATAATAGTGGAGGAGGAGAAGGCTGAAGAAACAGGGGAGGAGAAGAAGGCCGAGGATAAACTAGTTGTGGAGGAGAAAATCGCAAAGACACAGGAGGAAACACCTATGAAAACTGTGAAATTGGTGTTTGAAGAAGATATTCGATGGGCTCAATTGCCAATTGGTTGCACTCTACTGCAACTAAGGGAGGTTATACGTGATCGTTTCCCAACCTCCACGGCAGTCCTTATCAAGTACAGGGATGAAGAAGGTGATTTGGTAACAATTACTACCAATGAAGAATTGCGATTGGCTGAAACTTCGAACGAGTCACAGGGTTCTGTTAGATTTTATATATTTGAAGTTAGTCCAGAGAAAGACCCATTCTATGAAAGGCTTAACACTAATGAGGTTGCCAAGTGTAAAGTCGAACAAAGTAGCGTGTTTGAGAATGGTCATGTATTAAGAGCAAAAGAAATAAAGATGTCATCTTGCATTGACGACTGGATAATTCAATTTGCTCAGTTATTTATAAACCACGTAGGATTTGAATCTGGCCCATACTTGGATCTCCATGACCTTGGGATGAAGCTTTATACTGAGGCTGTGGAAGAGACAGTAACAAGTGAAGAAGCTCAGGGTCTTTTTGATTTAGCAGCAGAGAAGTTTCACGAGATGGCAGCCTTAGCATTGTTCTACTGGGGAAATGTTATCATGTCAAAGGCGAGGAAGAAGGTCTACTTCACAGATGGTGCTTCAAAAGCACCTTTGTTTGACCAGATCAAAGCTGCATATGATTGGGTCGAAAAAGAATATGCTGAAGCAGGAAGGATATATCAAATGGCGATCGAAATCAAACCAGACTTTTATGAAGGCTATCTAGCACTAGGGCAGCAACAGTTTGAGCAGGCAAAACTTTCTTGGCTTTATGCAGTTAGCAGCAATGTTGATTCGAAAACATTGCCTTGTACTGAGGTTCTGAAACTTTATAACAATGCTGAGGAGAACATGGAAACAGGCATGAAAATGTGGGAAGAATGGGAAGAGCAGCGTACTGGCGAATTCTCTAAATCGAGCAATGTTAAAACCCAGTTGCAGAAGATGGGATTAGATGGGCTGATTAAGGATATATCAGCTGCTGAGGCTGCAGAACAGGCTAAAAATATGAGGTCTCATATAAACCTCTTGTGGGGTACCATGCTCTATGAACGATCAATATTGGAATTTAAGATGGGGCTACCAGTGTGGCATGAATGTCTGGAAGTTGCAGTTGAGAAATTTGAGCTTGCTGGAGCTTCTGCAACAGATATCGCTGTTATGATAAAGAACCACTGCTCAAGCAACAACGCACATGAAGGTCTCGGGTTCAAAATTGATGAGATAGTACAGGCATGGAACGAGATGTATGAAGCTCGAAAGTTGCTAACTGGAGTTCCATCATTCCGCTTAGAGCCATTATTTAGGCGAAGGGTCTCGAAAATCTACCACGTCTTGGAGGAAGCTTGA

Coding sequence (CDS)

ATGGGGAAGCAAGGTGGTAAGAAGAAGCAGATTGGTGATAAGTTTCGTGAGGCGATTTCGAAACATCGCCAAGGTGGAGATGGTAGTCCAAAAAGTTATGATAAAGACCATGTTATTTTCATTACTATGTCTCAGGTTTTAAAGGAAGAGGGGAATAGATTGTTTCAGTCTAGAGATCTTGAAGGAGCAATATTGAAATATGAAAAGGCCCTCAAATTACTTCCAAGAAATCATATAGATGTATCCTATCTTCGGAGTAACATGGCAGCATGCTATATGCAGATGGGGCTTAGTGAGTATCCCCGGGCGGTTCATGAGTGCAATTTGGCTCTTGAAGTTACGCCGAAGTACAGTAAGGCATTGTTGAAGCGGGCAAGATGTTATGAAGCTTTGCATAGGCTGGACTTGGCTCTAAGAGATGTTAATGCAGTTTTAAACATGGAGCCAAAAAATATCATGGCTTTAGAAATATCAGAGAGACTAACAGAGGCACTTGAGATGAAAGGATCAAAGGATGTCGATGCTGAGATCAAACCGCCTCTTGATTTTGTTGAATTGCCTGCTGCAGTGTTGCCACAAAAGAGACCAAAAGAAAAGAGCCGAAAGAAGAAAAACAACCAAAAGACTAAGGAAACCATTGATGAGAAGAAGGCTGATGAAATTGTGGAGGAAAAGGCTGGTGATGAAATGGTTGAGGAGAAGGCTGACGAAATAATAGTGGAGGAGGAGAAGGCTGACGAAATAATAGTGGAGGAGGAGAAGGCTGAAGAAACAGGGGAGGAGAAGAAGGCCGAGGATAAACTAGTTGTGGAGGAGAAAATCGCAAAGACACAGGAGGAAACACCTATGAAAACTGTGAAATTGGTGTTTGAAGAAGATATTCGATGGGCTCAATTGCCAATTGGTTGCACTCTACTGCAACTAAGGGAGGTTATACGTGATCGTTTCCCAACCTCCACGGCAGTCCTTATCAAGTACAGGGATGAAGAAGGTGATTTGGTAACAATTACTACCAATGAAGAATTGCGATTGGCTGAAACTTCGAACGAGTCACAGGGTTCTGTTAGATTTTATATATTTGAAGTTAGTCCAGAGAAAGACCCATTCTATGAAAGGCTTAACACTAATGAGGTTGCCAAGTGTAAAGTCGAACAAAGTAGCGTGTTTGAGAATGGTCATGTATTAAGAGCAAAAGAAATAAAGATGTCATCTTGCATTGACGACTGGATAATTCAATTTGCTCAGTTATTTATAAACCACGTAGGATTTGAATCTGGCCCATACTTGGATCTCCATGACCTTGGGATGAAGCTTTATACTGAGGCTGTGGAAGAGACAGTAACAAGTGAAGAAGCTCAGGGTCTTTTTGATTTAGCAGCAGAGAAGTTTCACGAGATGGCAGCCTTAGCATTGTTCTACTGGGGAAATGTTATCATGTCAAAGGCGAGGAAGAAGGTCTACTTCACAGATGGTGCTTCAAAAGCACCTTTGTTTGACCAGATCAAAGCTGCATATGATTGGGTCGAAAAAGAATATGCTGAAGCAGGAAGGATATATCAAATGGCGATCGAAATCAAACCAGACTTTTATGAAGGCTATCTAGCACTAGGGCAGCAACAGTTTGAGCAGGCAAAACTTTCTTGGCTTTATGCAGTTAGCAGCAATGTTGATTCGAAAACATTGCCTTGTACTGAGGTTCTGAAACTTTATAACAATGCTGAGGAGAACATGGAAACAGGCATGAAAATGTGGGAAGAATGGGAAGAGCAGCGTACTGGCGAATTCTCTAAATCGAGCAATGTTAAAACCCAGTTGCAGAAGATGGGATTAGATGGGCTGATTAAGGATATATCAGCTGCTGAGGCTGCAGAACAGGCTAAAAATATGAGGTCTCATATAAACCTCTTGTGGGGTACCATGCTCTATGAACGATCAATATTGGAATTTAAGATGGGGCTACCAGTGTGGCATGAATGTCTGGAAGTTGCAGTTGAGAAATTTGAGCTTGCTGGAGCTTCTGCAACAGATATCGCTGTTATGATAAAGAACCACTGCTCAAGCAACAACGCACATGAAGGTCTCGGGTTCAAAATTGATGAGATAGTACAGGCATGGAACGAGATGTATGAAGCTCGAAAGTTGCTAACTGGAGTTCCATCATTCCGCTTAGAGCCATTATTTAGGCGAAGGGTCTCGAAAATCTACCACGTCTTGGAGGAAGCTTGA

Protein sequence

MGKQGGKKKQIGDKFREAISKHRQGGDGSPKSYDKDHVIFITMSQVLKEEGNRLFQSRDLEGAILKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAVHECNLALEVTPKYSKALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALEMKGSKDVDAEIKPPLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKAGDEMVEEKADEIIVEEEKADEIIVEEEKAEETGEEKKAEDKLVVEEKIAKTQEETPMKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITTNEELRLAETSNESQGSVRFYIFEVSPEKDPFYERLNTNEVAKCKVEQSSVFENGHVLRAKEIKMSSCIDDWIIQFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFDLAAEKFHEMAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGRIYQMAIEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEENMETGMKMWEEWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINLLWGTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA
Homology
BLAST of Lag0034259 vs. NCBI nr
Match: XP_022969128.1 (protein PHOX1-like [Cucurbita maxima] >XP_022969137.1 protein PHOX1-like [Cucurbita maxima] >XP_022969145.1 protein PHOX1-like [Cucurbita maxima])

HSP 1 Score: 1247.6 bits (3227), Expect = 0.0e+00
Identity = 658/764 (86.13%), Postives = 688/764 (90.05%), Query Frame = 0

Query: 1   MGKQGG-KKKQIGDKFREAISKHRQGGDGSPKSYDKDHVIFITMSQVLKEEGNRLFQSRD 60
           MGKQ G KKKQIGDKFRE I+KHRQ GDGSP + DKDH IFITMS  LKEEGNRLFQ+RD
Sbjct: 1   MGKQSGKKKKQIGDKFREVIAKHRQSGDGSPSNNDKDHAIFITMSLALKEEGNRLFQARD 60

Query: 61  LEGAILKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAVHECNLALEVTPKYSK 120
           LEGA+LKYEKALKLLPRNHIDVSYLRSNMAACYMQMG+SEYPRA+HECNLALEVTPKYSK
Sbjct: 61  LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGISEYPRAIHECNLALEVTPKYSK 120

Query: 121 ALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALEMKGSKDVDAEIKP 180
           ALLKRARCYEALHRLDLALRDVN VLNMEP NIMALEISERLT+ALE KGSK+ DAEIK 
Sbjct: 121 ALLKRARCYEALHRLDLALRDVNTVLNMEPNNIMALEISERLTKALETKGSKEDDAEIKL 180

Query: 181 PLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKAGDEMVE-EKADEI 240
           PLDFVELPA VLPQK  KEK+RKKKNNQKTKETIDEKKADEIVEEK G+E VE EKA   
Sbjct: 181 PLDFVELPAEVLPQKGSKEKNRKKKNNQKTKETIDEKKADEIVEEKVGNETVEVEKAGNE 240

Query: 241 IVEEEKA-DEII-------------------VEEEKAEETGEEKKAEDKLVVEEKIAKTQ 300
            VEEEKA +E +                   VEEEKA+ET EEKKAEDKLVVEEKIA+  
Sbjct: 241 TVEEEKAGNETVEEETAGNGTVAEEKAGNGTVEEEKAKETVEEKKAEDKLVVEEKIARAL 300

Query: 301 EETPMKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITT 360
           EETPMKTVKLVF EDIRWAQ+P+ CTLLQLRE IRDRFPT  AVLIKYRDEEGDLVTIT 
Sbjct: 301 EETPMKTVKLVFGEDIRWAQVPVDCTLLQLREAIRDRFPTCPAVLIKYRDEEGDLVTITA 360

Query: 361 NEELRLAETSNESQGSVRFYIFEVSPEKDPFYERL-NTNEVAKCKVEQSSVFENGHVLRA 420
           NEELR+AET+  SQ SVRFYIFEV PE+DPFYE+    +EVAKCKVEQSS+FENGH+LRA
Sbjct: 361 NEELRMAETAKASQASVRFYIFEVKPEQDPFYEKFKKDDEVAKCKVEQSSIFENGHLLRA 420

Query: 421 KEIKMSSCIDDWIIQFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFD 480
           KEIK SSCIDDWIIQFAQLFINHVGFESGP LDLHDLGMKLY+EAVEETVTSEEAQGLF 
Sbjct: 421 KEIKTSSCIDDWIIQFAQLFINHVGFESGPCLDLHDLGMKLYSEAVEETVTSEEAQGLFK 480

Query: 481 LAAEKFHEMAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGR 540
           LAAEKFHEM ALALFYWGNVIMS ARKKVYFTDGASK PLF+QIK+AYDWVE EY EAGR
Sbjct: 481 LAAEKFHEMTALALFYWGNVIMSSARKKVYFTDGASKGPLFEQIKSAYDWVENEYVEAGR 540

Query: 541 IYQMAIEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEENM 600
            YQMA+EIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVD KTLPCTEVLKLYN AEE+M
Sbjct: 541 KYQMAVEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDPKTLPCTEVLKLYNYAEESM 600

Query: 601 ETGMKMWEEWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINLLW 660
           ETGMKMWEEWEEQRT EFSK+S VKTQLQKMGLDGLIKDISA EAAEQAKNMRSHINLLW
Sbjct: 601 ETGMKMWEEWEEQRTSEFSKTSTVKTQLQKMGLDGLIKDISADEAAEQAKNMRSHINLLW 660

Query: 661 GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK 720
           GTMLYERSILEFKMG+PVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK
Sbjct: 661 GTMLYERSILEFKMGIPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK 720

Query: 721 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 742
           IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA
Sbjct: 721 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 764

BLAST of Lag0034259 vs. NCBI nr
Match: KAG6587865.1 (Protein PHOX1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021753.1 Protein PHOX1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1243.4 bits (3216), Expect = 0.0e+00
Identity = 657/764 (85.99%), Postives = 687/764 (89.92%), Query Frame = 0

Query: 1   MGKQGG-KKKQIGDKFREAISKHRQGGDGSPKSYDKDHVIFITMSQVLKEEGNRLFQSRD 60
           MGKQ G KKKQIGDKFRE I+KHRQ GDGSP + DKDH IFITMS  LKEEGNRLFQ+RD
Sbjct: 1   MGKQSGKKKKQIGDKFREVIAKHRQSGDGSPSNNDKDHAIFITMSLALKEEGNRLFQARD 60

Query: 61  LEGAILKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAVHECNLALEVTPKYSK 120
           LEGA+LKYEKALKLLPRNHIDVSYLRSNMAACYMQMG+SEYPRA+HECNLALEVTPKYSK
Sbjct: 61  LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGISEYPRAIHECNLALEVTPKYSK 120

Query: 121 ALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALEMKGSKDVDAEIKP 180
           ALLKRARCYEALHRLDLALRDVN VLNMEP NIMALEISERLT+ALE KGSK+ DAEIK 
Sbjct: 121 ALLKRARCYEALHRLDLALRDVNTVLNMEPNNIMALEISERLTKALETKGSKEDDAEIKL 180

Query: 181 PLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKAGDEMVE-EKADEI 240
           PLDFVELPA VLPQK  KEK+RKKKNNQKTKETI EKKADEIVEEK G+E VE EKA   
Sbjct: 181 PLDFVELPAEVLPQKGSKEKNRKKKNNQKTKETIIEKKADEIVEEKVGNETVEVEKAGNE 240

Query: 241 IVEEEKA-DEII-------------------VEEEKAEETGEEKKAEDKLVVEEKIAKTQ 300
            VEEEKA +E +                   VEEEKA+E+ EEKKAEDKLVVEEKIA+  
Sbjct: 241 TVEEEKAGNETVEEETAGNGTVAEEKAGNGTVEEEKAKESVEEKKAEDKLVVEEKIARAL 300

Query: 301 EETPMKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITT 360
           EETPMKTVKLVF EDIRWAQ+P+ CTLLQLRE IRDRFPT  AVLIKYRDEEGDLVTIT 
Sbjct: 301 EETPMKTVKLVFGEDIRWAQVPVDCTLLQLREAIRDRFPTCPAVLIKYRDEEGDLVTITA 360

Query: 361 NEELRLAETSNESQGSVRFYIFEVSPEKDPFYERL-NTNEVAKCKVEQSSVFENGHVLRA 420
           NEELR+AET+  SQ SVRFYIFEV PE+DPFYE+    +EVAKCKVEQSS+FENGH+LRA
Sbjct: 361 NEELRMAETAKASQASVRFYIFEVKPEQDPFYEKFKKDDEVAKCKVEQSSIFENGHLLRA 420

Query: 421 KEIKMSSCIDDWIIQFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFD 480
           KEIK SSCIDDWIIQFAQLFINHVGFESGP LDLHDLGMKLY+EAVEETVTSEEAQGLF 
Sbjct: 421 KEIKTSSCIDDWIIQFAQLFINHVGFESGPCLDLHDLGMKLYSEAVEETVTSEEAQGLFK 480

Query: 481 LAAEKFHEMAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGR 540
           LAAEKFHEM ALALFYWGNVIMS ARKKVYFTDGASK PLF+QIK+AYDWVE EY EAGR
Sbjct: 481 LAAEKFHEMTALALFYWGNVIMSSARKKVYFTDGASKGPLFEQIKSAYDWVENEYVEAGR 540

Query: 541 IYQMAIEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEENM 600
            YQMA+EIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVD KTLPCTEVLKLYN AEE+M
Sbjct: 541 KYQMAVEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDPKTLPCTEVLKLYNYAEESM 600

Query: 601 ETGMKMWEEWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINLLW 660
           ETGMKMWEEWEEQRT EFSK+S VKTQLQKMGLDGLIKDISA EAAEQAKNMRSHINLLW
Sbjct: 601 ETGMKMWEEWEEQRTSEFSKTSTVKTQLQKMGLDGLIKDISADEAAEQAKNMRSHINLLW 660

Query: 661 GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK 720
           GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK
Sbjct: 661 GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK 720

Query: 721 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 742
           IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA
Sbjct: 721 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 764

BLAST of Lag0034259 vs. NCBI nr
Match: XP_022932878.1 (protein PHOX1-like [Cucurbita moschata] >XP_022932887.1 protein PHOX1-like [Cucurbita moschata] >XP_022932896.1 protein PHOX1-like [Cucurbita moschata])

HSP 1 Score: 1242.6 bits (3214), Expect = 0.0e+00
Identity = 656/764 (85.86%), Postives = 687/764 (89.92%), Query Frame = 0

Query: 1   MGKQGG-KKKQIGDKFREAISKHRQGGDGSPKSYDKDHVIFITMSQVLKEEGNRLFQSRD 60
           MGKQ G KKKQIGDKFRE I+KHRQ GDGSP + DKDH IFITMS  LKEEGNRLFQ+RD
Sbjct: 1   MGKQSGKKKKQIGDKFREVIAKHRQSGDGSPSNNDKDHAIFITMSLALKEEGNRLFQARD 60

Query: 61  LEGAILKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAVHECNLALEVTPKYSK 120
           LEGA+LKYEKALKLLPRNHIDVSYLRSNMAACYMQMG+SEYPRA+HECNLALEVTPKYSK
Sbjct: 61  LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGISEYPRAIHECNLALEVTPKYSK 120

Query: 121 ALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALEMKGSKDVDAEIKP 180
           ALLKRARCYEALHRLDLALRDVN VLNMEP NIMALEISERLT+ALE KGSK+ DAEIK 
Sbjct: 121 ALLKRARCYEALHRLDLALRDVNTVLNMEPNNIMALEISERLTKALETKGSKEDDAEIKL 180

Query: 181 PLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKAGDEMVE-EKADEI 240
           PLDFVELPA VLPQK  KEK+RKKKNNQKTKETI EKKADEIVEEK G+E VE EKA   
Sbjct: 181 PLDFVELPAEVLPQKGSKEKNRKKKNNQKTKETIIEKKADEIVEEKVGNETVEVEKAGNE 240

Query: 241 IVEEEKA-DEII-------------------VEEEKAEETGEEKKAEDKLVVEEKIAKTQ 300
            VEEEKA +E +                   VEEEKA+E+ EEKKAEDKLVVEEKIA+  
Sbjct: 241 TVEEEKAGNETVEEETAGNGTVAEEKAGNGTVEEEKAKESVEEKKAEDKLVVEEKIARAL 300

Query: 301 EETPMKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITT 360
           EETPMKTVKLVF EDIRWAQ+P+ CTLLQLRE IRDRFPT  AVLIKYRDEEGDLVTIT 
Sbjct: 301 EETPMKTVKLVFGEDIRWAQVPVDCTLLQLREAIRDRFPTCPAVLIKYRDEEGDLVTITA 360

Query: 361 NEELRLAETSNESQGSVRFYIFEVSPEKDPFYERL-NTNEVAKCKVEQSSVFENGHVLRA 420
           NEELR+AET+  SQ SVRFYIFEV PE+DPFYE+    +EVAKCKVEQSS+FENGH+LRA
Sbjct: 361 NEELRMAETAKASQASVRFYIFEVKPEQDPFYEKFKKDDEVAKCKVEQSSIFENGHLLRA 420

Query: 421 KEIKMSSCIDDWIIQFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFD 480
           KEIK SSCIDDWIIQFAQLFINHVGFESGP LDLHDLGMKLY+EAVEETVTSEEAQGLF 
Sbjct: 421 KEIKTSSCIDDWIIQFAQLFINHVGFESGPCLDLHDLGMKLYSEAVEETVTSEEAQGLFK 480

Query: 481 LAAEKFHEMAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGR 540
           LAAEKFHEM ALALFYWGNVIMS ARKKVYFTDGASK PLF+Q+K+AYDWVE EY EAGR
Sbjct: 481 LAAEKFHEMTALALFYWGNVIMSSARKKVYFTDGASKEPLFEQVKSAYDWVENEYVEAGR 540

Query: 541 IYQMAIEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEENM 600
            YQMA+EIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVD KTLPCTEVLKLYN AEE+M
Sbjct: 541 KYQMAVEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDPKTLPCTEVLKLYNYAEESM 600

Query: 601 ETGMKMWEEWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINLLW 660
           ETGMKMWEEWEEQRT EFSK+S VKTQLQKMGLDGLIKDISA EAAEQAKNMRSHINLLW
Sbjct: 601 ETGMKMWEEWEEQRTSEFSKTSTVKTQLQKMGLDGLIKDISADEAAEQAKNMRSHINLLW 660

Query: 661 GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK 720
           GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK
Sbjct: 661 GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK 720

Query: 721 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 742
           IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA
Sbjct: 721 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 764

BLAST of Lag0034259 vs. NCBI nr
Match: XP_038879044.1 (protein PHOX1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1241.1 bits (3210), Expect = 0.0e+00
Identity = 648/745 (86.98%), Postives = 695/745 (93.29%), Query Frame = 0

Query: 1   MGKQGG-KKKQIGDKFREAISKHRQGGDGSPKSYDKDHVIFITMSQVLKEEGNRLFQSRD 60
           MGKQ G KKKQIGDKFREA+SKHRQ GDG+P SYDKDHVIFITMSQ+LKEEGN+LFQSRD
Sbjct: 1   MGKQSGKKKKQIGDKFREAVSKHRQNGDGNP-SYDKDHVIFITMSQILKEEGNKLFQSRD 60

Query: 61  LEGAILKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAVHECNLALEVTPKYSK 120
           LEGA+LKY+KALKLLPRNHIDVSYLRSNMAACYMQMGL+EYPRA+HECNLALEVTPKYSK
Sbjct: 61  LEGAMLKYDKALKLLPRNHIDVSYLRSNMAACYMQMGLTEYPRAIHECNLALEVTPKYSK 120

Query: 121 ALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALEMKGSKDVDAEIKP 180
           ALLKRARCYE LHRLDLALRDV AVLNMEP NIMALEISERLT+ALEMKGSK+ DA+IK 
Sbjct: 121 ALLKRARCYEGLHRLDLALRDVKAVLNMEPNNIMALEISERLTKALEMKGSKEEDADIKL 180

Query: 181 PLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIV--EEKAGDEMV-EEKAD 240
           PLDFVELP+ + PQKR KEK+RKKKNNQKTKETIDEKKADE V  EEK  + MV EEK D
Sbjct: 181 PLDFVELPSTMSPQKR-KEKNRKKKNNQKTKETIDEKKADETVEGEEKPDEIMVGEEKVD 240

Query: 241 EIIVEEEKADEIIVEEEKAEETGEEKKAEDKLVVEEKIAKTQEETPMKTVKLVFEEDIRW 300
           E +VEEEK DE +VEEEK +E  EEKKAEDKLVVEEKI +TQEETPMKTVKLVF EDIRW
Sbjct: 241 E-MVEEEKVDE-MVEEEKVDEMVEEKKAEDKLVVEEKITRTQEETPMKTVKLVFGEDIRW 300

Query: 301 AQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITTNEELRLAETSNESQGSVR 360
           AQLPI CTLLQLREVIRDRFPT TAVLIKYRDEEGDLVTITTNEELRLAETS ESQGSVR
Sbjct: 301 AQLPIDCTLLQLREVIRDRFPTCTAVLIKYRDEEGDLVTITTNEELRLAETSKESQGSVR 360

Query: 361 FYIFEVSPEKDPFYERLNTNEVAKCKVEQSSVFENGHVLRAKEIKMSSCIDDWIIQFAQL 420
           FYIFEV+PE+DPFYER   NEVAKC+VE++S+FENGHVL+ KEIKMSSCI+DWIIQFAQL
Sbjct: 361 FYIFEVNPEQDPFYERFKNNEVAKCEVEENSIFENGHVLKPKEIKMSSCINDWIIQFAQL 420

Query: 421 FINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFDLAAEKFHEMAALALFYWGN 480
           FINHVGF+SGPYLDLHDLGMKLY+EAVEETVTSEEAQGLF+LAAEKFHEMAALALF WGN
Sbjct: 421 FINHVGFDSGPYLDLHDLGMKLYSEAVEETVTSEEAQGLFELAAEKFHEMAALALFNWGN 480

Query: 481 VIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGRIYQMAIEIKPDFYEGYLAL 540
           VIMS+ARKKVYF DG SK  + +QIKAA+DWVEKEYAEA R YQMA+EIKPDFYEGYLAL
Sbjct: 481 VIMSRARKKVYFADGGSKVHVLEQIKAAFDWVEKEYAEAERKYQMAVEIKPDFYEGYLAL 540

Query: 541 GQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEENMETGMKMWEEWEEQRTGEFS 600
           G+QQFEQAK+SW YAVSS+VD KT PCT+V++LYN+AEENMETGMK+WEEWEEQRTGE S
Sbjct: 541 GKQQFEQAKVSWHYAVSSDVDPKTWPCTKVMQLYNSAEENMETGMKLWEEWEEQRTGELS 600

Query: 601 KSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINLLWGTMLYERSILEFKMGLPVW 660
           KSSNV+TQLQKMGLDGLIKDIS  EAAEQAKNMRSHINLLWGTMLYERSILEFKMGLP W
Sbjct: 601 KSSNVRTQLQKMGLDGLIKDISVDEAAEQAKNMRSHINLLWGTMLYERSILEFKMGLPTW 660

Query: 661 HECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIDEIVQAWNEMYEARKLLT 720
           HECLEVAVEKFELAGASATDIAVMIKNHCSSNN+HEGLGFKIDEIVQAWNEMYEARKLL+
Sbjct: 661 HECLEVAVEKFELAGASATDIAVMIKNHCSSNNSHEGLGFKIDEIVQAWNEMYEARKLLS 720

Query: 721 GVPSFRLEPLFRRRVSKIYHVLEEA 742
           GVPSFRLEPLFRRRVSKIYHVLE+A
Sbjct: 721 GVPSFRLEPLFRRRVSKIYHVLEQA 741

BLAST of Lag0034259 vs. NCBI nr
Match: XP_023530345.1 (protein PHOX1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1239.9 bits (3207), Expect = 0.0e+00
Identity = 654/764 (85.60%), Postives = 686/764 (89.79%), Query Frame = 0

Query: 1   MGKQGG-KKKQIGDKFREAISKHRQGGDGSPKSYDKDHVIFITMSQVLKEEGNRLFQSRD 60
           MGKQ G KKKQIGDKFRE I+KHRQ GDGSP + DKDH IFITMS  LKEEGNRLFQ+RD
Sbjct: 1   MGKQSGKKKKQIGDKFREVIAKHRQSGDGSPSNNDKDHAIFITMSLALKEEGNRLFQARD 60

Query: 61  LEGAILKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAVHECNLALEVTPKYSK 120
           LEGA+LKYEKALKLLPRNHIDVSYLRSNMAACYMQMG+SEYPRA+HECNLALEVTPKYSK
Sbjct: 61  LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGISEYPRAIHECNLALEVTPKYSK 120

Query: 121 ALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALEMKGSKDVDAEIKP 180
           ALLKRARCYEALHRLDLALRDVN VLNMEP NIMALEISERLT+ALE KGSK+ DAEIK 
Sbjct: 121 ALLKRARCYEALHRLDLALRDVNTVLNMEPNNIMALEISERLTKALETKGSKEDDAEIKL 180

Query: 181 PLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKAGDEMVE-EKADEI 240
           PLDFVELPA VLPQK  KEK+RKKKNNQKTKETIDEKKADEIVEEK G+E VE EKA   
Sbjct: 181 PLDFVELPAEVLPQKGSKEKNRKKKNNQKTKETIDEKKADEIVEEKVGNETVEVEKAGNE 240

Query: 241 IVEEEKA-DEII-------------------VEEEKAEETGEEKKAEDKLVVEEKIAKTQ 300
            VEEEKA +E +                   VEEEKA+E+ EEKKAEDKLVVEEKIA+  
Sbjct: 241 TVEEEKAGNETVEEETAGNGTVAEEKAGNGTVEEEKAKESVEEKKAEDKLVVEEKIARAL 300

Query: 301 EETPMKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITT 360
           EETPMKTVKLVF EDIRWAQ+P+ CTLLQLRE IRDRFPT  AVLIKYRDEEGDLVTIT 
Sbjct: 301 EETPMKTVKLVFGEDIRWAQVPVDCTLLQLREAIRDRFPTCPAVLIKYRDEEGDLVTITA 360

Query: 361 NEELRLAETSNESQGSVRFYIFEVSPEKDPFYERL-NTNEVAKCKVEQSSVFENGHVLRA 420
           NEELR+AET+  SQ SVRFYIFEV PE+DPFYE+    +EVAKCKVEQSS+FENGH+LRA
Sbjct: 361 NEELRMAETAKASQASVRFYIFEVKPEQDPFYEKFKKDDEVAKCKVEQSSIFENGHLLRA 420

Query: 421 KEIKMSSCIDDWIIQFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFD 480
           KEIK SSCIDDWIIQFAQLFINHVGFESGP LDLHDLGMKLY+EAVEETVTSEEAQGLF 
Sbjct: 421 KEIKTSSCIDDWIIQFAQLFINHVGFESGPCLDLHDLGMKLYSEAVEETVTSEEAQGLFK 480

Query: 481 LAAEKFHEMAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGR 540
           LAAEKFHEM ALALFYWGNVIMS ARKKVYFTDGASK PLF+QIK+AYDWVE +Y EAGR
Sbjct: 481 LAAEKFHEMTALALFYWGNVIMSSARKKVYFTDGASKGPLFEQIKSAYDWVENKYVEAGR 540

Query: 541 IYQMAIEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEENM 600
            YQMA+EIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVD KTL CTEVLKLYN AEE+M
Sbjct: 541 KYQMAVEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDPKTLSCTEVLKLYNYAEESM 600

Query: 601 ETGMKMWEEWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINLLW 660
           ETGMKMWEEWEEQRT EFSK+S VKTQLQKMGLDGLIKDISA EA EQAKNMRSHINLLW
Sbjct: 601 ETGMKMWEEWEEQRTSEFSKTSTVKTQLQKMGLDGLIKDISADEATEQAKNMRSHINLLW 660

Query: 661 GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK 720
           GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEG+GFK
Sbjct: 661 GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGIGFK 720

Query: 721 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 742
           IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA
Sbjct: 721 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 764

BLAST of Lag0034259 vs. ExPASy Swiss-Prot
Match: F4IRM4 (Protein PHOX1 OS=Arabidopsis thaliana OX=3702 GN=PHOX1 PE=1 SV=1)

HSP 1 Score: 719.2 bits (1855), Expect = 4.9e-206
Identity = 408/754 (54.11%), Postives = 522/754 (69.23%), Query Frame = 0

Query: 1   MGKQGGKKKQIGDKFREAISKHRQGGD--------GSPKSYDKDHVIFITMSQVLKEEGN 60
           MGK  GKKK               GG          + KS+D D  IFI  +  LKEEGN
Sbjct: 1   MGKPTGKKKNNNYTEMPPTESSTTGGGKTGKSFDRSATKSFDDDMTIFINRALELKEEGN 60

Query: 61  RLFQSRDLEGAILKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAVHECNLALE 120
           +LFQ RD EGA+ +Y+KA+KLLPR+H DV+YLR++MA+CYMQMGL EYP A++ECNLALE
Sbjct: 61  KLFQKRDYEGAMFRYDKAVKLLPRDHGDVAYLRTSMASCYMQMGLGEYPNAINECNLALE 120

Query: 121 VTPKYSKALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALEMKGSKD 180
            +P++SKALLKRARCYEAL++LD A RD   VLNMEP+N+ A EI ER+ + L  KG  D
Sbjct: 121 ASPRFSKALLKRARCYEALNKLDFAFRDSRVVLNMEPENVSANEIFERVKKVLVGKGI-D 180

Query: 181 VDAEIKPPLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKAGDEMVE 240
           VD E++  L  V+   A   +K  KE+ RKKK    T                 G    E
Sbjct: 181 VD-EMEKNLVNVQPVGAARLRKIVKERLRKKKKKSMTMTN--------------GGNDGE 240

Query: 241 EKADEIIVEEEKADE-IIVEEEKAEETGEEKKAEDKLVVEEK--IAK--TQEETPMKTVK 300
            K+ E +VE+ K D    V+  +  +  EEKK EDK+ V +K  IA    ++ T  +TVK
Sbjct: 241 RKSVEAVVEDAKVDNGEEVDSGRKGKAIEEKKLEDKVAVMDKEVIASEIKEDATVTRTVK 300

Query: 301 LVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITTNEELRLAET 360
           LV  +DIRWAQLP+  +++ +R+VI+DRFP     LIKYRD EGDLVTITT +ELRLA +
Sbjct: 301 LVHGDDIRWAQLPLDSSVVLVRDVIKDRFPALKGFLIKYRDSEGDLVTITTTDELRLAAS 360

Query: 361 SNESQGSVRFYIFEVSPEKDPFYERLNTNE-VAKCKVEQSSVFENGHVLRAKEI-KMSSC 420
           + E  GS R YI EVSP ++P Y+ ++ +E   K     SSV +NG V    E  K S+ 
Sbjct: 361 TREKLGSFRLYIAEVSPNQEPTYDVIDNDESTDKFAKGSSSVADNGSVGDFVESEKASTS 420

Query: 421 IDDWIIQFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFDLAAEKFHE 480
           ++ WI QFAQLF NHVGF+S  YL+LH+LGMKLYTEA+E+ VT E+AQ LFD+AA+KF E
Sbjct: 421 LEHWIFQFAQLFKNHVGFDSDSYLELHNLGMKLYTEAMEDIVTGEDAQELFDIAADKFQE 480

Query: 481 MAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGRIYQMAIEI 540
           MAALA+F WGNV MSKAR+++YF +  S+  + ++++A ++W + EY +A   Y+ A++I
Sbjct: 481 MAALAMFNWGNVHMSKARRQIYFPEDGSRETILEKVEAGFEWAKNEYNKAAEKYEGAVKI 540

Query: 541 KPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEENMETGMKMWE 600
           K DFYE  LALGQQQFEQAKL W +A+S  VD ++    +VLKLYN AEE+ME GM++WE
Sbjct: 541 KSDFYEALLALGQQQFEQAKLCWYHALSGEVDIESDASQDVLKLYNKAEESMEKGMQIWE 600

Query: 601 EWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINLLWGTMLYERS 660
           E EE+R    S     K  LQK+GLDG+  + S  E+AEQ  NM S INLLWG++LYERS
Sbjct: 601 EMEERRLNGISNFDKHKELLQKLGLDGIFSEASDEESAEQTANMSSQINLLWGSLLYERS 660

Query: 661 ILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIDEIVQAW 720
           I+E+K+GLP W ECLEVAVEKFELAGASATDIAVM+KNHCSS+NA EG+GFKIDEIVQAW
Sbjct: 661 IVEYKLGLPTWDECLEVAVEKFELAGASATDIAVMVKNHCSSDNALEGMGFKIDEIVQAW 720

Query: 721 NEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLE 740
           NEMY+A++   GVPSFRLEPLFRRR  K++ +LE
Sbjct: 721 NEMYDAKRWQIGVPSFRLEPLFRRRSPKLHDILE 738

BLAST of Lag0034259 vs. ExPASy Swiss-Prot
Match: F4JTI1 (Protein PHOX4 OS=Arabidopsis thaliana OX=3702 GN=PHOX4 PE=2 SV=1)

HSP 1 Score: 706.1 bits (1821), Expect = 4.3e-202
Identity = 407/778 (52.31%), Postives = 530/778 (68.12%), Query Frame = 0

Query: 1   MGKQGGKKKQIGDKFREAISKHRQGGDGSPKSY--------DKDHVIFITMSQVLKEEGN 60
           MGK   KKK   +  ++A      GG  S K+Y        D+D  IFI+ +  LKEEGN
Sbjct: 1   MGKPTAKKKN-PETPKDASGGGGGGGGKSGKTYHRSTSRVFDEDMEIFISRALELKEEGN 60

Query: 61  RLFQSRDLEGAILKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAVHECNLALE 120
           +LFQ RD EGA+L ++KALKLLP++HIDV+YLR++MA+CYMQMGL EYP A+ ECNLALE
Sbjct: 61  KLFQKRDHEGAMLSFDKALKLLPKDHIDVAYLRTSMASCYMQMGLGEYPNAISECNLALE 120

Query: 121 VTPKYSKALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALEMKGSKD 180
            +P+YSKAL++R+RCYEAL++LD A RD   VLNMEP N+ A EI +R+ + L  KG  D
Sbjct: 121 ASPRYSKALVRRSRCYEALNKLDYAFRDARIVLNMEPGNVSANEIFDRVKKVLVDKGI-D 180

Query: 181 VDAEIKPPLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKAGDEM-- 240
           VD   K  +D   + AA L +K  KE+ RK K  +K+    +E K+ ++V    GDE   
Sbjct: 181 VDEMEKDFVDVQPVCAARL-KKIVKERLRKSKKKKKSGGKDEELKSPKVVVVDKGDEAEG 240

Query: 241 ----VEEKADEIIVE-------EE-----KADEIIVEEEKAEETGEEKKAEDKLVVEEKI 300
                EEK+D+  ++       EE     K+D+   ++    + GEE+K EDK+VV +K 
Sbjct: 241 RNKPKEEKSDKSDIDGKIGGKREEKKTSFKSDKGQKKKSGGNKAGEERKVEDKVVVMDKE 300

Query: 301 -----------AKTQEETPMKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVL 360
                      +K +  T  +T+KLV  +DIRWAQLP+  T+  +R+VIRDRFP     L
Sbjct: 301 VIASEIVDGGGSKKEGATVTRTIKLVHGDDIRWAQLPLDSTVRLVRDVIRDRFPALRGFL 360

Query: 361 IKYRDEEGDLVTITTNEELRLAETSNESQGSVRFYIFEVSPEKDPFYERL-NTNEVAKCK 420
           IKYRD EGDLVTITT +ELRLA ++++  GS+R YI EV+P+++P Y+ + NT    K  
Sbjct: 361 IKYRDTEGDLVTITTTDELRLAASTHDKLGSLRLYIAEVNPDQEPTYDGMSNTESTDKVS 420

Query: 421 VEQSSVFENGHVLR-AKEIKMSSCIDDWIIQFAQLFINHVGFESGPYLDLHDLGMKLYTE 480
              SS+ +NG V       K S C ++WI QFAQLF NHVGF+S  Y+DLHDLGMKLYTE
Sbjct: 421 KRLSSLADNGSVGEYVGSDKASGCFENWIFQFAQLFKNHVGFDSDSYVDLHDLGMKLYTE 480

Query: 481 AVEETVTSEEAQGLFDLAAEKFHEMAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQI 540
           A+E+ VT E+AQ LF +AA+KF EM ALAL  WGNV MSKARK+V   + AS+  + + +
Sbjct: 481 AMEDAVTGEDAQELFQIAADKFQEMGALALLNWGNVHMSKARKQVCIPEDASREAIIEAV 540

Query: 541 KAAYDWVEKEYAEAGRIYQMAIEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTL 600
           +AA+ W + EY +A   Y+ AI++KPDFYE  LALGQ+QFE AKL W +A+ S VD ++ 
Sbjct: 541 EAAFVWTQNEYNKAAEKYEEAIKVKPDFYEALLALGQEQFEHAKLCWYHALKSKVDLESE 600

Query: 601 PCTEVLKLYNNAEENMETGMKMWEEWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAE 660
              EVLKLYN AE++ME GM++WEE EE R    SK    K  L+K+ LD L  + S  E
Sbjct: 601 ASQEVLKLYNKAEDSMERGMQIWEEMEECRLNGISKLDKHKNMLRKLELDELFSEASEEE 660

Query: 661 AAEQAKNMRSHINLLWGTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMI 720
             EQ  NM S INLLWG++LYERSI+E+K+GLP W ECLEVAVEKFELAGASATDIAVM+
Sbjct: 661 TVEQTANMSSQINLLWGSLLYERSIVEYKLGLPTWDECLEVAVEKFELAGASATDIAVMV 720

Query: 721 KNHCSSNNAHEGLGFKIDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLE 740
           KNHCSS +A EG+GFKIDEIVQAWNEMY+A++   GVPSFRLEP+FRRR  K++ +LE
Sbjct: 721 KNHCSSESALEGMGFKIDEIVQAWNEMYDAKRWQMGVPSFRLEPMFRRRAPKLHDILE 775

BLAST of Lag0034259 vs. ExPASy Swiss-Prot
Match: K7TQE3 (HSP-interacting protein OS=Zea mays OX=4577 GN=HIP PE=1 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 2.2e-158
Identity = 324/736 (44.02%), Postives = 468/736 (63.59%), Query Frame = 0

Query: 34  DKDHVIFITMSQVLKEEGNRLFQSRDLEGAILKYEKALKLLPR-NHIDVSYLRSNMAACY 93
           D D  +F+ +S+ LKEEG RLF  RD EGA  KY+KA++LLP    ++ ++LR+++A CY
Sbjct: 16  DGDDAVFLELSRELKEEGTRLFNRRDFEGAAFKYDKAVQLLPAGRRVEAAHLRASIAHCY 75

Query: 94  MQMGLSEYPRAVHECNLALEVTPKYSKALLKRARCYEALHRLDLALRDVNAVLNMEPKNI 153
           M+M  +E+  A+HECNLALE  P+YS+ALL+RA C+EAL R DLA  D+  VL  EP N 
Sbjct: 76  MRMSPAEFHHAIHECNLALEAVPRYSRALLRRAACFEALGRPDLAWGDIRTVLRWEPGNR 135

Query: 154 MALEISERLTEALEMKGSKDVDAEIKPPLDFVELPAAVLPQKRPKEKSRKKKNNQKTKET 213
            A +IS+R+  ALE KG   V  ++ P  D  E+ +A       K + RKK  N++    
Sbjct: 136 AARQISDRVRTALEDKGI-SVALDVLPE-DENEIASA-------KGEERKKSRNKRFDSV 195

Query: 214 IDEKKADEIV--------EEKAGDEMVEEKADEIIVEEEKADEIIVEEEKAEETGEE--- 273
              ++ +  +        E++AG        +     E+     + + E++ ETGE+   
Sbjct: 196 AGGREGENGIALLESASTEKQAGPRQTNGTGNHQDHTEDSESNGLEKLEQSTETGEKDMG 255

Query: 274 ------------KKAEDKLVVEEKIAKTQE-----ETPMKTVKLVFEEDIRWAQLPIGCT 333
                       +  E K      +   Q+     E  MK VKLVF EDIR AQ+P  C+
Sbjct: 256 KKRGAHAAGKKPRCGESKQQKHSAVNHCQDNIGAKEEVMKDVKLVFGEDIRCAQMPANCS 315

Query: 334 LLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITTNEELRLAETSNESQGSVRFYIFEVSP 393
           L QLRE+++++FP+  A LIKY+D+E DLVTIT +EEL  A     SQ  +RFY+ EV+ 
Sbjct: 316 LPQLREIVQNKFPSLKAFLIKYKDKEEDLVTITLSEELSWASNLAVSQVPIRFYVVEVNH 375

Query: 394 EKDPFYERLNTN-EVAKCKVEQSSVFENGHVLRAKEIKMSSCIDDWIIQFAQLFINHVGF 453
            ++   + +      A  +  +  + +NG +    +++     DDW++QFAQ+F NHVGF
Sbjct: 376 VQELGVDGVRRRPSFATLERNRDIMLDNGTI--GHDVEHKHYADDWMVQFAQIFKNHVGF 435

Query: 454 ESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFDLAAEKFHEMAALALFYWGNVIMSKAR 513
            S  YLDLHDLG++L+ EA+E+T+  EEAQ +F++A  KF EMAALALF  GNV MS+AR
Sbjct: 436 SSDAYLDLHDLGLRLHYEAMEDTIQREEAQEIFEVAESKFKEMAALALFNCGNVHMSRAR 495

Query: 514 KKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGRIYQMAIEIKPDFYEGYLALGQQQFEQ 573
           ++    +   +  + +++  +YDW   EYA+AG +++ A++ K DF+EG +ALGQQ+FEQ
Sbjct: 496 RRPCLAEDPLQEFILEKVNVSYDWACTEYAKAGAMFEEAVKTKSDFFEGLIALGQQKFEQ 555

Query: 574 AKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEENMETGMKMWEEWEEQRTGEFSKSSNVKT 633
           AKLSW YA++  ++ +    TEVL+L+N+AE+NME GM MWE  E  R    SK S  K 
Sbjct: 556 AKLSWYYALACKINME----TEVLELFNHAEDNMEKGMDMWERMETLRLKGLSKPSKEKV 615

Query: 634 QLQKMGLDGLIKDISAAEAAEQAKNMRSHINLLWGTMLYERSILEFKMGLPVWHECLEVA 693
            L+KM L+G +KDISA EA EQA ++RSHIN+LWGT+LYERS++EF +GLP W E L VA
Sbjct: 616 VLEKMVLEGFVKDISADEAFEQASSIRSHINILWGTILYERSVVEFNLGLPSWEESLTVA 675

Query: 694 VEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIDEIVQAWNEMYEARKLLTGVPSFRL 740
           +EKF++ GAS  DI V++KNHC++    EGL FK++EIVQAW+EM++A+   +G   FRL
Sbjct: 676 MEKFKIGGASQADINVIVKNHCANETTQEGLSFKVEEIVQAWSEMHDAKNWRSGPLYFRL 735

BLAST of Lag0034259 vs. ExPASy Swiss-Prot
Match: F4K487 (Protein PHOX3 OS=Arabidopsis thaliana OX=3702 GN=PHOX3 PE=1 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 1.5e-146
Identity = 301/703 (42.82%), Postives = 459/703 (65.29%), Query Frame = 0

Query: 41  ITMSQVLKEEGNRLFQSRDLEGAILKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEY 100
           ++ +Q LKEEGN+LFQ RD +GA+ KY +A+K+LP++H++VS++R+N+A+CYMQ+   E+
Sbjct: 123 VSKAQGLKEEGNKLFQKRDYDGAMFKYGEAIKILPKDHVEVSHVRANVASCYMQLEPGEF 182

Query: 101 PRAVHECNLALEVTPKYSKALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISER 160
            +A+HEC+LAL VTP ++KALLKRARCYEAL++LDLALRDV  V  ++PKN MA EI E+
Sbjct: 183 AKAIHECDLALSVTPDHNKALLKRARCYEALNKLDLALRDVCMVSKLDPKNPMASEIVEK 242

Query: 161 LTEALEMKGSKDVDAEIKPPLDFVE----LPAAVLPQKRPKEKSRKKKNNQKTKETIDEK 220
           L   LE KG +  ++ I+ P D+VE     PAA+  +       + KK+NQ     ++EK
Sbjct: 243 LKRTLESKGLRINNSVIELPPDYVEPVGASPAALWAKLGKVRVKKTKKSNQ-----VEEK 302

Query: 221 KADEIVEEKAGDEMVEEKADEIIVEEEKAD-EIIVEEEKAEETGEEKKAEDKLVVEEKIA 280
              E      G+++  EK + ++ E+ K   ++ V+ +++++  +  K ++K+++EE++ 
Sbjct: 303 SEGE------GEDVEPEKKNNVLAEKGKEKIKMKVKGKQSDKRSDTSKEQEKVIIEEELL 362

Query: 281 KTQEETPMKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVT 340
               E   K VK V+ +DIR A+LPI CTL +LREV+ +RFP+  AV IKYRD+EGDLVT
Sbjct: 363 VIGVEDVNKDVKFVYSDDIRLAELPINCTLFKLREVVHERFPSLRAVHIKYRDQEGDLVT 422

Query: 341 ITTNEELRLAETSNESQGSVRFYIFEVSPEKDPFYERLNTNEVAKCKVEQSSVFENGHVL 400
           ITT+EELR++E S+ SQG++RFY+ EVSPE+DPF+ RL   E+ K K+   S        
Sbjct: 423 ITTDEELRMSEVSSRSQGTMRFYVVEVSPEQDPFFGRL--VEMKKLKITADS-------F 482

Query: 401 RAKEIKMSSC-IDDWIIQFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQG 460
           +AK      C ++DW+I+FA LF      +S   L+L +LGMKL +EA+EE VTS+ AQG
Sbjct: 483 KAKVNGRGGCKVEDWMIEFAHLFKIQARIDSDRCLNLQELGMKLNSEAMEEVVTSDAAQG 542

Query: 461 LFDLAAEKFHEMAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAE 520
            FD AA++F E+AA +L   G V MS ARK++    G S   + +Q+K AY+  +KE+A 
Sbjct: 543 PFDRAAQQFQEVAARSLLNLGYVHMSGARKRLSLLQGVSGESVSEQVKTAYECAKKEHAN 602

Query: 521 AGRIYQMAIEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAE 580
           A   Y+ A++IKP+ +E +LALG QQFE+A+LSW Y + S++D KT P  +V++ Y +AE
Sbjct: 603 AKEKYEEAMKIKPECFEVFLALGLQQFEEARLSWYYVLVSHLDLKTWPYADVVQFYQSAE 662

Query: 581 ENMETGMKMWEEWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHIN 640
            N++  M++ E  E  +  E S++        +  L    ++      A++A  ++S I+
Sbjct: 663 SNIKKSMEVLENLETGKESEPSQAGKTDCLTHEKDLGSSTQN----NPAKEAGRLKSWID 722

Query: 641 LLWGTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGL 700
           +L   +LYERSI+E+K+  P W E LE A+EKFELAG    D+  +I     + N    +
Sbjct: 723 ILLCAVLYERSIMEYKLDQPFWRESLEAAMEKFELAGTCKDDVVEIISEDYVAGNTLRDI 782

Query: 701 GFKIDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHV 738
            F ++EI+Q ++E+YEA+    G+PS +LE + +RR   I+HV
Sbjct: 783 RFHMEEIIQIFDEIYEAKHWTNGIPSDQLEEILKRRAENIFHV 801

BLAST of Lag0034259 vs. ExPASy Swiss-Prot
Match: O48802 (Protein CLMP1 OS=Arabidopsis thaliana OX=3702 GN=CLMP1 PE=1 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 7.8e-119
Identity = 290/750 (38.67%), Postives = 416/750 (55.47%), Query Frame = 0

Query: 1   MGKQGGKKKQIG--DKFREAISKHRQGGDGSPKS-----YDKDHVIFITMSQVLKEEGNR 60
           MGK GG+KK+ G  +     ++     G   P +      D D  IF+  +  LKEEGN+
Sbjct: 1   MGKSGGRKKKSGGSNSNSSQVNSSETSGLSKPSTIVNGGVDFDASIFLKRAHELKEEGNK 60

Query: 61  LFQSRDLEGAILKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAVHECNLALEV 120
            FQ+RD  GA+ +YE  +KL+P++H D +   SN AAC MQM   +Y   + EC++AL+ 
Sbjct: 61  KFQARDYVGALEQYENGIKLIPKSHPDRAVFHSNRAACLMQMKPIDYESVISECSMALKS 120

Query: 121 TPKYSKALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALEMKGSKDV 180
            P +++ALL+RAR +EA+ + DLA++DVN +L  +P +  A EIS+RL  AL     +D+
Sbjct: 121 QPGFTRALLRRARAFEAVGKFDLAVQDVNVLLGSDPNHKDAGEISKRLKTAL--GPHQDL 180

Query: 181 DAEIKPPLDFVEL----PAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKAGDE 240
            +   P           P A L    P     KK              ++  VE      
Sbjct: 181 QSRPSPAALGASAALGGPIAGLGPCLPSRNVHKKGVTSPVGSVSLPNASNGKVERPQVVN 240

Query: 241 MVEEKADEIIVEEEKADEIIVEE-------EKAEETGEEKKAEDKLVVEEKIAKTQEETP 300
            V E      V + +A  ++++         K EE G    A    V E++I        
Sbjct: 241 PVTENGGS--VSKGQASRVVLKPVSHSPKGSKVEELGSSSVAVVGKVQEKRIR------- 300

Query: 301 MKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITTNEEL 360
            + +K V++ DIR  Q+P+ C   +LRE++  RFP+S AVLIKY+D +GDLVTIT+  EL
Sbjct: 301 WRPLKFVYDHDIRLGQMPVNCRFKELREIVSSRFPSSKAVLIKYKDNDGDLVTITSTAEL 360

Query: 361 RLAETS-------------NESQGSVRFYIFEVSPEKDPFY---------ERLNTNEVAK 420
           +LAE++             ++S G +R ++ +VSPE++P           E+    EV  
Sbjct: 361 KLAESAADCILTKEPDTDKSDSVGMLRLHVVDVSPEQEPMLLEEEEEEVEEKPVIEEVIS 420

Query: 421 CKVEQSSVFENGHVLRAKEI---KMSSC---------IDDWIIQFAQLFINHVGFESGPY 480
              E  S  E       KE+   K SS          +DDW+  FA LF  HVG +   +
Sbjct: 421 SPTESLSETEINTEKTDKEVEKEKASSSEDPETKELEMDDWLFDFAHLFRTHVGIDPDAH 480

Query: 481 LDLHDLGMKLYTEAVEETVTSEEAQGLFDLAAEKFHEMAALALFYWGNVIMSKARKKVYF 540
           +DLH+LGM+L +EA+EETVTSE+AQ LFD A+ KF E+AALA F WGNV M  ARK++  
Sbjct: 481 IDLHELGMELCSEALEETVTSEKAQPLFDKASAKFQEVAALAFFNWGNVHMCAARKRIPL 540

Query: 541 TDGASKAPLFDQIKAAYDWVEKEYAEAGRIYQMAIEIKPDFYEGYLALGQQQFEQAKLSW 600
            + A K  +  Q++ AY+WV++ Y  A   Y+ A+ IKPDFYEG LALGQQQFE AKL W
Sbjct: 541 DESAGKEVVAAQLQTAYEWVKERYTLAKEKYEQALSIKPDFYEGLLALGQQQFEMAKLHW 600

Query: 601 LYAVSSNVDSKTLPCTEVLKLYNNAEENMETGMKMWEEWEEQRTGEF-----SKSSNVKT 660
            Y ++  +D      +E L L+++AE  M+   +MWE+ EEQR  +      +K   V  
Sbjct: 601 SYLLAQKIDISGWDPSETLNLFDSAEAKMKDATEMWEKLEEQRMDDLKNPNSNKKEEVSK 660

Query: 661 QLQKMGLDG---LIKDISAAEAAEQAKNMRSHINLLWGTMLYERSILEFKMGLPVWHECL 691
           + +K G DG   + + I+A EAAEQA  MRS I+L WG ML+ERS +E K+G   W++ L
Sbjct: 661 RRKKQGGDGNEEVSETITAEEAAEQATAMRSQIHLFWGNMLFERSQVECKIGKDGWNKNL 720

BLAST of Lag0034259 vs. ExPASy TrEMBL
Match: A0A6J1HVI3 (protein PHOX1-like OS=Cucurbita maxima OX=3661 GN=LOC111468214 PE=4 SV=1)

HSP 1 Score: 1247.6 bits (3227), Expect = 0.0e+00
Identity = 658/764 (86.13%), Postives = 688/764 (90.05%), Query Frame = 0

Query: 1   MGKQGG-KKKQIGDKFREAISKHRQGGDGSPKSYDKDHVIFITMSQVLKEEGNRLFQSRD 60
           MGKQ G KKKQIGDKFRE I+KHRQ GDGSP + DKDH IFITMS  LKEEGNRLFQ+RD
Sbjct: 1   MGKQSGKKKKQIGDKFREVIAKHRQSGDGSPSNNDKDHAIFITMSLALKEEGNRLFQARD 60

Query: 61  LEGAILKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAVHECNLALEVTPKYSK 120
           LEGA+LKYEKALKLLPRNHIDVSYLRSNMAACYMQMG+SEYPRA+HECNLALEVTPKYSK
Sbjct: 61  LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGISEYPRAIHECNLALEVTPKYSK 120

Query: 121 ALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALEMKGSKDVDAEIKP 180
           ALLKRARCYEALHRLDLALRDVN VLNMEP NIMALEISERLT+ALE KGSK+ DAEIK 
Sbjct: 121 ALLKRARCYEALHRLDLALRDVNTVLNMEPNNIMALEISERLTKALETKGSKEDDAEIKL 180

Query: 181 PLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKAGDEMVE-EKADEI 240
           PLDFVELPA VLPQK  KEK+RKKKNNQKTKETIDEKKADEIVEEK G+E VE EKA   
Sbjct: 181 PLDFVELPAEVLPQKGSKEKNRKKKNNQKTKETIDEKKADEIVEEKVGNETVEVEKAGNE 240

Query: 241 IVEEEKA-DEII-------------------VEEEKAEETGEEKKAEDKLVVEEKIAKTQ 300
            VEEEKA +E +                   VEEEKA+ET EEKKAEDKLVVEEKIA+  
Sbjct: 241 TVEEEKAGNETVEEETAGNGTVAEEKAGNGTVEEEKAKETVEEKKAEDKLVVEEKIARAL 300

Query: 301 EETPMKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITT 360
           EETPMKTVKLVF EDIRWAQ+P+ CTLLQLRE IRDRFPT  AVLIKYRDEEGDLVTIT 
Sbjct: 301 EETPMKTVKLVFGEDIRWAQVPVDCTLLQLREAIRDRFPTCPAVLIKYRDEEGDLVTITA 360

Query: 361 NEELRLAETSNESQGSVRFYIFEVSPEKDPFYERL-NTNEVAKCKVEQSSVFENGHVLRA 420
           NEELR+AET+  SQ SVRFYIFEV PE+DPFYE+    +EVAKCKVEQSS+FENGH+LRA
Sbjct: 361 NEELRMAETAKASQASVRFYIFEVKPEQDPFYEKFKKDDEVAKCKVEQSSIFENGHLLRA 420

Query: 421 KEIKMSSCIDDWIIQFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFD 480
           KEIK SSCIDDWIIQFAQLFINHVGFESGP LDLHDLGMKLY+EAVEETVTSEEAQGLF 
Sbjct: 421 KEIKTSSCIDDWIIQFAQLFINHVGFESGPCLDLHDLGMKLYSEAVEETVTSEEAQGLFK 480

Query: 481 LAAEKFHEMAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGR 540
           LAAEKFHEM ALALFYWGNVIMS ARKKVYFTDGASK PLF+QIK+AYDWVE EY EAGR
Sbjct: 481 LAAEKFHEMTALALFYWGNVIMSSARKKVYFTDGASKGPLFEQIKSAYDWVENEYVEAGR 540

Query: 541 IYQMAIEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEENM 600
            YQMA+EIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVD KTLPCTEVLKLYN AEE+M
Sbjct: 541 KYQMAVEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDPKTLPCTEVLKLYNYAEESM 600

Query: 601 ETGMKMWEEWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINLLW 660
           ETGMKMWEEWEEQRT EFSK+S VKTQLQKMGLDGLIKDISA EAAEQAKNMRSHINLLW
Sbjct: 601 ETGMKMWEEWEEQRTSEFSKTSTVKTQLQKMGLDGLIKDISADEAAEQAKNMRSHINLLW 660

Query: 661 GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK 720
           GTMLYERSILEFKMG+PVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK
Sbjct: 661 GTMLYERSILEFKMGIPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK 720

Query: 721 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 742
           IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA
Sbjct: 721 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 764

BLAST of Lag0034259 vs. ExPASy TrEMBL
Match: A0A6J1EXN1 (protein PHOX1-like OS=Cucurbita moschata OX=3662 GN=LOC111439405 PE=4 SV=1)

HSP 1 Score: 1242.6 bits (3214), Expect = 0.0e+00
Identity = 656/764 (85.86%), Postives = 687/764 (89.92%), Query Frame = 0

Query: 1   MGKQGG-KKKQIGDKFREAISKHRQGGDGSPKSYDKDHVIFITMSQVLKEEGNRLFQSRD 60
           MGKQ G KKKQIGDKFRE I+KHRQ GDGSP + DKDH IFITMS  LKEEGNRLFQ+RD
Sbjct: 1   MGKQSGKKKKQIGDKFREVIAKHRQSGDGSPSNNDKDHAIFITMSLALKEEGNRLFQARD 60

Query: 61  LEGAILKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAVHECNLALEVTPKYSK 120
           LEGA+LKYEKALKLLPRNHIDVSYLRSNMAACYMQMG+SEYPRA+HECNLALEVTPKYSK
Sbjct: 61  LEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGISEYPRAIHECNLALEVTPKYSK 120

Query: 121 ALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALEMKGSKDVDAEIKP 180
           ALLKRARCYEALHRLDLALRDVN VLNMEP NIMALEISERLT+ALE KGSK+ DAEIK 
Sbjct: 121 ALLKRARCYEALHRLDLALRDVNTVLNMEPNNIMALEISERLTKALETKGSKEDDAEIKL 180

Query: 181 PLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKAGDEMVE-EKADEI 240
           PLDFVELPA VLPQK  KEK+RKKKNNQKTKETI EKKADEIVEEK G+E VE EKA   
Sbjct: 181 PLDFVELPAEVLPQKGSKEKNRKKKNNQKTKETIIEKKADEIVEEKVGNETVEVEKAGNE 240

Query: 241 IVEEEKA-DEII-------------------VEEEKAEETGEEKKAEDKLVVEEKIAKTQ 300
            VEEEKA +E +                   VEEEKA+E+ EEKKAEDKLVVEEKIA+  
Sbjct: 241 TVEEEKAGNETVEEETAGNGTVAEEKAGNGTVEEEKAKESVEEKKAEDKLVVEEKIARAL 300

Query: 301 EETPMKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITT 360
           EETPMKTVKLVF EDIRWAQ+P+ CTLLQLRE IRDRFPT  AVLIKYRDEEGDLVTIT 
Sbjct: 301 EETPMKTVKLVFGEDIRWAQVPVDCTLLQLREAIRDRFPTCPAVLIKYRDEEGDLVTITA 360

Query: 361 NEELRLAETSNESQGSVRFYIFEVSPEKDPFYERL-NTNEVAKCKVEQSSVFENGHVLRA 420
           NEELR+AET+  SQ SVRFYIFEV PE+DPFYE+    +EVAKCKVEQSS+FENGH+LRA
Sbjct: 361 NEELRMAETAKASQASVRFYIFEVKPEQDPFYEKFKKDDEVAKCKVEQSSIFENGHLLRA 420

Query: 421 KEIKMSSCIDDWIIQFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFD 480
           KEIK SSCIDDWIIQFAQLFINHVGFESGP LDLHDLGMKLY+EAVEETVTSEEAQGLF 
Sbjct: 421 KEIKTSSCIDDWIIQFAQLFINHVGFESGPCLDLHDLGMKLYSEAVEETVTSEEAQGLFK 480

Query: 481 LAAEKFHEMAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGR 540
           LAAEKFHEM ALALFYWGNVIMS ARKKVYFTDGASK PLF+Q+K+AYDWVE EY EAGR
Sbjct: 481 LAAEKFHEMTALALFYWGNVIMSSARKKVYFTDGASKEPLFEQVKSAYDWVENEYVEAGR 540

Query: 541 IYQMAIEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEENM 600
            YQMA+EIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVD KTLPCTEVLKLYN AEE+M
Sbjct: 541 KYQMAVEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDPKTLPCTEVLKLYNYAEESM 600

Query: 601 ETGMKMWEEWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINLLW 660
           ETGMKMWEEWEEQRT EFSK+S VKTQLQKMGLDGLIKDISA EAAEQAKNMRSHINLLW
Sbjct: 601 ETGMKMWEEWEEQRTSEFSKTSTVKTQLQKMGLDGLIKDISADEAAEQAKNMRSHINLLW 660

Query: 661 GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK 720
           GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK
Sbjct: 661 GTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFK 720

Query: 721 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 742
           IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA
Sbjct: 721 IDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 764

BLAST of Lag0034259 vs. ExPASy TrEMBL
Match: A0A6J1BZI4 (protein PHOX1-like OS=Momordica charantia OX=3673 GN=LOC111007136 PE=4 SV=1)

HSP 1 Score: 1222.2 bits (3161), Expect = 0.0e+00
Identity = 639/754 (84.75%), Postives = 685/754 (90.85%), Query Frame = 0

Query: 1   MGKQGG-KKKQIGDKFREAISKHRQGGDGSPKSYDKDHVIFITMSQVLKEEGNRLFQSRD 60
           MGKQ G KKKQIGDKFREAISK RQGGD S +SYDKD+ IFI MSQ LKEEGN+LFQSRD
Sbjct: 1   MGKQSGKKKKQIGDKFREAISKQRQGGDSSLRSYDKDNSIFIIMSQALKEEGNKLFQSRD 60

Query: 61  LEGAILKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAVHECNLALEVTPKYSK 120
           LEGA+ KYEKALKLLP+NHIDVSYLRSNMAACYMQMGLSEYPRA+HECNLALEVTPKYSK
Sbjct: 61  LEGAMFKYEKALKLLPKNHIDVSYLRSNMAACYMQMGLSEYPRAIHECNLALEVTPKYSK 120

Query: 121 ALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALEMKGSKDVDAEIKP 180
           ALLKRARCYEALHRLDLALRDVN VLNMEPKNIMALEIS+R+T+ALEMK S + DAEI+ 
Sbjct: 121 ALLKRARCYEALHRLDLALRDVNTVLNMEPKNIMALEISDRVTKALEMKKSNEDDAEIQL 180

Query: 181 PLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKAGDEM-VEEK---- 240
           PLDFVELP+ VLPQK  KEK+RKKK NQKTKETI EKK DE VEE+  DE+ V EK    
Sbjct: 181 PLDFVELPSPVLPQKIHKEKNRKKK-NQKTKETIGEKKTDETVEEEKADEIKVREKETDE 240

Query: 241 -------ADEIIVEEEKADEIIVEEEKAEETGEEKKAEDKLVVEEKIAKTQEETPMKTVK 300
                  ADEI V E+KADEII  E++A+ET EEKKAEDKLVVEEKI +T EETPMK VK
Sbjct: 241 IIVRDKEADEITVREKKADEIIAGEKRADETVEEKKAEDKLVVEEKIRRTHEETPMKAVK 300

Query: 301 LVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITTNEELRLAET 360
           LVF EDIRWAQLPI CTLL+LREVIRDRFPT  AVLIKYRDEEGDLVTITTNEELRLAE 
Sbjct: 301 LVFGEDIRWAQLPIDCTLLRLREVIRDRFPTCRAVLIKYRDEEGDLVTITTNEELRLAEA 360

Query: 361 SNESQGSVRFYIFEVSPEKDPFYERLNTNEVAKCKVEQSSVFENGHVLRAKEIKMSSCID 420
           + ESQGSVRFYIFEV+PE+DPFY R   +EV KCKVEQ+S+FENGHVLR KEI MSSCID
Sbjct: 361 TTESQGSVRFYIFEVNPEQDPFYGRFKNDEVEKCKVEQNSMFENGHVLRGKEIMMSSCID 420

Query: 421 DWIIQFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFDLAAEKFHEMA 480
           DWI+QFAQLFINHVGFESG YLDL +LGMKLY+EA+EETVTSEEAQGLF++AAEKFHEMA
Sbjct: 421 DWIVQFAQLFINHVGFESGSYLDLQELGMKLYSEAMEETVTSEEAQGLFEIAAEKFHEMA 480

Query: 481 ALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGRIYQMAIEIKP 540
           ALA+FYWGNVIMS+ARKKVYFTD ASKAP+F+QIKAAYDWV+KEY EAG+ YQ+A+EIKP
Sbjct: 481 ALAMFYWGNVIMSRARKKVYFTDSASKAPVFEQIKAAYDWVKKEYVEAGQKYQIAVEIKP 540

Query: 541 DFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEENMETGMKMWEEW 600
           DFYEGYLALGQQQFEQAKLSW YAVSSNVD KT PCTEVL+LYNNAE+NMETGMK+WEEW
Sbjct: 541 DFYEGYLALGQQQFEQAKLSWQYAVSSNVDLKTWPCTEVLQLYNNAEDNMETGMKIWEEW 600

Query: 601 EEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINLLWGTMLYERSIL 660
           EEQR  E SKSSNVKTQLQKMGLDGLIKDISA EAAEQAKNMRSHINLLWGTMLYERSIL
Sbjct: 601 EEQRASELSKSSNVKTQLQKMGLDGLIKDISADEAAEQAKNMRSHINLLWGTMLYERSIL 660

Query: 661 EFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIDEIVQAWNE 720
           EFK+GLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIDEIVQAWNE
Sbjct: 661 EFKLGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIDEIVQAWNE 720

Query: 721 MYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 742
           MYEARKLLTGVPSFRLEPLFRRRVSKIYH+LEEA
Sbjct: 721 MYEARKLLTGVPSFRLEPLFRRRVSKIYHILEEA 753

BLAST of Lag0034259 vs. ExPASy TrEMBL
Match: A0A6J1JHE2 (protein PHOX1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485121 PE=4 SV=1)

HSP 1 Score: 1215.3 bits (3143), Expect = 0.0e+00
Identity = 633/742 (85.31%), Postives = 684/742 (92.18%), Query Frame = 0

Query: 1   MGKQ-GGKKKQIGDKFREAISKHRQGGDGSPKSYDKDHVIFITMSQVLKEEGNRLFQSRD 60
           MGKQ G KKKQIGDK+REAISK RQGG GS  SYDKDHVIFITMSQ LKEEGN+LFQSRD
Sbjct: 1   MGKQTGKKKKQIGDKYREAISKRRQGGVGS-SSYDKDHVIFITMSQALKEEGNKLFQSRD 60

Query: 61  LEGAILKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAVHECNLALEVTPKYSK 120
           +EGA+LKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYP+A+HECNLALEVTPKYSK
Sbjct: 61  VEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPQAIHECNLALEVTPKYSK 120

Query: 121 ALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALEMKGSKDVDAEIKP 180
           ALLKRARCYEALHRLDLALRDVNAVLN+EP+NIMALEISERLT+AL M+GSK+ DAEIK 
Sbjct: 121 ALLKRARCYEALHRLDLALRDVNAVLNLEPRNIMALEISERLTKALMMQGSKEDDAEIKL 180

Query: 181 PLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKAGDEMVEEKADEII 240
           PLDFVELP+++LPQ+RPKEK+RK KNNQKTKETIDEKKA E VEEKA + +VEE+  +  
Sbjct: 181 PLDFVELPSSLLPQERPKEKNRKMKNNQKTKETIDEKKAHETVEEKADEIIVEEEKADET 240

Query: 241 VEEEKADEIIVEEEKAEETGEEKKAEDKLVVEEKIAKTQEETPMKTVKLVFEEDIRWAQL 300
           VEE+KADEIIVEEEKA+ET EEKK EDKLVVEEKI +TQEETPMK+VKLVF +DIRWAQ+
Sbjct: 241 VEEKKADEIIVEEEKADETVEEKKPEDKLVVEEKINRTQEETPMKSVKLVFGDDIRWAQV 300

Query: 301 PIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITTNEELRLAETSNESQGSVRFYI 360
           PI CTLLQLREVI+DRFPT TAVLIKYRDEEGDLVTIT +EELRLAETS ESQGSVRFYI
Sbjct: 301 PIDCTLLQLREVIQDRFPTCTAVLIKYRDEEGDLVTITADEELRLAETSKESQGSVRFYI 360

Query: 361 FEVSPEKDPFYERLNTNEVAKCKVEQSSVFENGHVLRAKEIKMSSCIDDWIIQFAQLFIN 420
           FEV+PE+DPFY R   +E +KCKVE     EN +VLRAKE+K+SSCIDDW+I FAQLFIN
Sbjct: 361 FEVNPEQDPFYGRFKNDEDSKCKVE-----ENSYVLRAKEMKISSCIDDWLIHFAQLFIN 420

Query: 421 HVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFDLAAEKFHEMAALALFYWGNVIM 480
           HVGFESGPYLDLHDLGMKLY+EAVEETVTSEEAQGLF+ AAEKFHEMAALALFYWGNVIM
Sbjct: 421 HVGFESGPYLDLHDLGMKLYSEAVEETVTSEEAQGLFESAAEKFHEMAALALFYWGNVIM 480

Query: 481 SKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGRIYQMAIEIKPDFYEGYLALGQQ 540
           S+ARKKVYFTDG SK  L +QIKAAYDWVEK Y EAG  YQMA++IKPDFYEGYLALGQQ
Sbjct: 481 SRARKKVYFTDGVSKVSLSEQIKAAYDWVEKAYVEAGNKYQMAVKIKPDFYEGYLALGQQ 540

Query: 541 QFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEENMETGMKMWEEWEEQRTGEFSKSS 600
           QFEQAKLSW YAVSSNVD KT PCT+V+ LYNNAE+NMETGM+MWEEWE+Q T E SKSS
Sbjct: 541 QFEQAKLSWHYAVSSNVDPKTWPCTKVIHLYNNAEDNMETGMRMWEEWEKQHTAELSKSS 600

Query: 601 NVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINLLWGTMLYERSILEFKMGLPVWHEC 660
           +V+T LQKMGLDGLIKDISA EAAEQAKNMRSHINLLWGTMLYERSILEFKMGLP WHEC
Sbjct: 601 DVETPLQKMGLDGLIKDISADEAAEQAKNMRSHINLLWGTMLYERSILEFKMGLPSWHEC 660

Query: 661 LEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIDEIVQAWNEMYEARKLLTGVP 720
           LEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKI+EIVQAWNEMYEARKLLTGVP
Sbjct: 661 LEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIEEIVQAWNEMYEARKLLTGVP 720

Query: 721 SFRLEPLFRRRVSKIYHVLEEA 742
           SFRLEPLFRRRVSKIYHVLE+A
Sbjct: 721 SFRLEPLFRRRVSKIYHVLEQA 736

BLAST of Lag0034259 vs. ExPASy TrEMBL
Match: A0A6J1JJD6 (protein PHOX1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485121 PE=4 SV=1)

HSP 1 Score: 1213.4 bits (3138), Expect = 0.0e+00
Identity = 642/761 (84.36%), Postives = 688/761 (90.41%), Query Frame = 0

Query: 1   MGKQ-GGKKKQIGDKFREAISKHRQGGDGSPKSYDKDHVIFITMSQVLKEEGNRLFQSRD 60
           MGKQ G KKKQIGDK+REAISK RQGG GS  SYDKDHVIFITMSQ LKEEGN+LFQSRD
Sbjct: 1   MGKQTGKKKKQIGDKYREAISKRRQGGVGS-SSYDKDHVIFITMSQALKEEGNKLFQSRD 60

Query: 61  LEGAILKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAVHECNLALEVTPKYSK 120
           +EGA+LKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYP+A+HECNLALEVTPKYSK
Sbjct: 61  VEGAMLKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPQAIHECNLALEVTPKYSK 120

Query: 121 ALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALEMKGSKDVDAEIKP 180
           ALLKRARCYEALHRLDLALRDVNAVLN+EP+NIMALEISERLT+AL M+GSK+ DAEIK 
Sbjct: 121 ALLKRARCYEALHRLDLALRDVNAVLNLEPRNIMALEISERLTKALMMQGSKEDDAEIKL 180

Query: 181 PLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKA---------GDEM 240
           PLDFVELP+++LPQ+RPKEK+RK KNNQKTKETIDEKKA E VEEKA          DE 
Sbjct: 181 PLDFVELPSSLLPQERPKEKNRKMKNNQKTKETIDEKKAHETVEEKADEIIVEEEKADET 240

Query: 241 VEE-KADEIIVEEE---------KADEIIVEEEKAEETGEEKKAEDKLVVEEKIAKTQEE 300
           VEE KADEIIVEEE         KADEIIVEEEKA+ET EEKK EDKLVVEEKI +TQEE
Sbjct: 241 VEEKKADEIIVEEEKADETVEEKKADEIIVEEEKADETVEEKKPEDKLVVEEKINRTQEE 300

Query: 301 TPMKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITTNE 360
           TPMK+VKLVF +DIRWAQ+PI CTLLQLREVI+DRFPT TAVLIKYRDEEGDLVTIT +E
Sbjct: 301 TPMKSVKLVFGDDIRWAQVPIDCTLLQLREVIQDRFPTCTAVLIKYRDEEGDLVTITADE 360

Query: 361 ELRLAETSNESQGSVRFYIFEVSPEKDPFYERLNTNEVAKCKVEQSSVFENGHVLRAKEI 420
           ELRLAETS ESQGSVRFYIFEV+PE+DPFY R   +E +KCKVE     EN +VLRAKE+
Sbjct: 361 ELRLAETSKESQGSVRFYIFEVNPEQDPFYGRFKNDEDSKCKVE-----ENSYVLRAKEM 420

Query: 421 KMSSCIDDWIIQFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFDLAA 480
           K+SSCIDDW+I FAQLFINHVGFESGPYLDLHDLGMKLY+EAVEETVTSEEAQGLF+ AA
Sbjct: 421 KISSCIDDWLIHFAQLFINHVGFESGPYLDLHDLGMKLYSEAVEETVTSEEAQGLFESAA 480

Query: 481 EKFHEMAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGRIYQ 540
           EKFHEMAALALFYWGNVIMS+ARKKVYFTDG SK  L +QIKAAYDWVEK Y EAG  YQ
Sbjct: 481 EKFHEMAALALFYWGNVIMSRARKKVYFTDGVSKVSLSEQIKAAYDWVEKAYVEAGNKYQ 540

Query: 541 MAIEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEENMETG 600
           MA++IKPDFYEGYLALGQQQFEQAKLSW YAVSSNVD KT PCT+V+ LYNNAE+NMETG
Sbjct: 541 MAVKIKPDFYEGYLALGQQQFEQAKLSWHYAVSSNVDPKTWPCTKVIHLYNNAEDNMETG 600

Query: 601 MKMWEEWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINLLWGTM 660
           M+MWEEWE+Q T E SKSS+V+T LQKMGLDGLIKDISA EAAEQAKNMRSHINLLWGTM
Sbjct: 601 MRMWEEWEKQHTAELSKSSDVETPLQKMGLDGLIKDISADEAAEQAKNMRSHINLLWGTM 660

Query: 661 LYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIDE 720
           LYERSILEFKMGLP WHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKI+E
Sbjct: 661 LYERSILEFKMGLPSWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIEE 720

Query: 721 IVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEEA 742
           IVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLE+A
Sbjct: 721 IVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLEQA 755

BLAST of Lag0034259 vs. TAIR 10
Match: AT2G25290.1 (Octicosapeptide/Phox/Bem1p (PB1) domain-containing protein / tetratricopeptide repeat (TPR)-containing protein )

HSP 1 Score: 719.2 bits (1855), Expect = 3.5e-207
Identity = 408/754 (54.11%), Postives = 522/754 (69.23%), Query Frame = 0

Query: 1   MGKQGGKKKQIGDKFREAISKHRQGGD--------GSPKSYDKDHVIFITMSQVLKEEGN 60
           MGK  GKKK               GG          + KS+D D  IFI  +  LKEEGN
Sbjct: 1   MGKPTGKKKNNNYTEMPPTESSTTGGGKTGKSFDRSATKSFDDDMTIFINRALELKEEGN 60

Query: 61  RLFQSRDLEGAILKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAVHECNLALE 120
           +LFQ RD EGA+ +Y+KA+KLLPR+H DV+YLR++MA+CYMQMGL EYP A++ECNLALE
Sbjct: 61  KLFQKRDYEGAMFRYDKAVKLLPRDHGDVAYLRTSMASCYMQMGLGEYPNAINECNLALE 120

Query: 121 VTPKYSKALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALEMKGSKD 180
            +P++SKALLKRARCYEAL++LD A RD   VLNMEP+N+ A EI ER+ + L  KG  D
Sbjct: 121 ASPRFSKALLKRARCYEALNKLDFAFRDSRVVLNMEPENVSANEIFERVKKVLVGKGI-D 180

Query: 181 VDAEIKPPLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKAGDEMVE 240
           VD E++  L  V+   A   +K  KE+ RKKK    T                 G    E
Sbjct: 181 VD-EMEKNLVNVQPVGAARLRKIVKERLRKKKKKSMTMTN--------------GGNDGE 240

Query: 241 EKADEIIVEEEKADE-IIVEEEKAEETGEEKKAEDKLVVEEK--IAK--TQEETPMKTVK 300
            K+ E +VE+ K D    V+  +  +  EEKK EDK+ V +K  IA    ++ T  +TVK
Sbjct: 241 RKSVEAVVEDAKVDNGEEVDSGRKGKAIEEKKLEDKVAVMDKEVIASEIKEDATVTRTVK 300

Query: 301 LVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITTNEELRLAET 360
           LV  +DIRWAQLP+  +++ +R+VI+DRFP     LIKYRD EGDLVTITT +ELRLA +
Sbjct: 301 LVHGDDIRWAQLPLDSSVVLVRDVIKDRFPALKGFLIKYRDSEGDLVTITTTDELRLAAS 360

Query: 361 SNESQGSVRFYIFEVSPEKDPFYERLNTNE-VAKCKVEQSSVFENGHVLRAKEI-KMSSC 420
           + E  GS R YI EVSP ++P Y+ ++ +E   K     SSV +NG V    E  K S+ 
Sbjct: 361 TREKLGSFRLYIAEVSPNQEPTYDVIDNDESTDKFAKGSSSVADNGSVGDFVESEKASTS 420

Query: 421 IDDWIIQFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFDLAAEKFHE 480
           ++ WI QFAQLF NHVGF+S  YL+LH+LGMKLYTEA+E+ VT E+AQ LFD+AA+KF E
Sbjct: 421 LEHWIFQFAQLFKNHVGFDSDSYLELHNLGMKLYTEAMEDIVTGEDAQELFDIAADKFQE 480

Query: 481 MAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGRIYQMAIEI 540
           MAALA+F WGNV MSKAR+++YF +  S+  + ++++A ++W + EY +A   Y+ A++I
Sbjct: 481 MAALAMFNWGNVHMSKARRQIYFPEDGSRETILEKVEAGFEWAKNEYNKAAEKYEGAVKI 540

Query: 541 KPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEENMETGMKMWE 600
           K DFYE  LALGQQQFEQAKL W +A+S  VD ++    +VLKLYN AEE+ME GM++WE
Sbjct: 541 KSDFYEALLALGQQQFEQAKLCWYHALSGEVDIESDASQDVLKLYNKAEESMEKGMQIWE 600

Query: 601 EWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINLLWGTMLYERS 660
           E EE+R    S     K  LQK+GLDG+  + S  E+AEQ  NM S INLLWG++LYERS
Sbjct: 601 EMEERRLNGISNFDKHKELLQKLGLDGIFSEASDEESAEQTANMSSQINLLWGSLLYERS 660

Query: 661 ILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIDEIVQAW 720
           I+E+K+GLP W ECLEVAVEKFELAGASATDIAVM+KNHCSS+NA EG+GFKIDEIVQAW
Sbjct: 661 IVEYKLGLPTWDECLEVAVEKFELAGASATDIAVMVKNHCSSDNALEGMGFKIDEIVQAW 720

Query: 721 NEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLE 740
           NEMY+A++   GVPSFRLEPLFRRR  K++ +LE
Sbjct: 721 NEMYDAKRWQIGVPSFRLEPLFRRRSPKLHDILE 738

BLAST of Lag0034259 vs. TAIR 10
Match: AT2G25290.2 (Octicosapeptide/Phox/Bem1p (PB1) domain-containing protein / tetratricopeptide repeat (TPR)-containing protein )

HSP 1 Score: 719.2 bits (1855), Expect = 3.5e-207
Identity = 408/754 (54.11%), Postives = 522/754 (69.23%), Query Frame = 0

Query: 1   MGKQGGKKKQIGDKFREAISKHRQGGD--------GSPKSYDKDHVIFITMSQVLKEEGN 60
           MGK  GKKK               GG          + KS+D D  IFI  +  LKEEGN
Sbjct: 1   MGKPTGKKKNNNYTEMPPTESSTTGGGKTGKSFDRSATKSFDDDMTIFINRALELKEEGN 60

Query: 61  RLFQSRDLEGAILKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAVHECNLALE 120
           +LFQ RD EGA+ +Y+KA+KLLPR+H DV+YLR++MA+CYMQMGL EYP A++ECNLALE
Sbjct: 61  KLFQKRDYEGAMFRYDKAVKLLPRDHGDVAYLRTSMASCYMQMGLGEYPNAINECNLALE 120

Query: 121 VTPKYSKALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALEMKGSKD 180
            +P++SKALLKRARCYEAL++LD A RD   VLNMEP+N+ A EI ER+ + L  KG  D
Sbjct: 121 ASPRFSKALLKRARCYEALNKLDFAFRDSRVVLNMEPENVSANEIFERVKKVLVGKGI-D 180

Query: 181 VDAEIKPPLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKAGDEMVE 240
           VD E++  L  V+   A   +K  KE+ RKKK    T                 G    E
Sbjct: 181 VD-EMEKNLVNVQPVGAARLRKIVKERLRKKKKKSMTMTN--------------GGNDGE 240

Query: 241 EKADEIIVEEEKADE-IIVEEEKAEETGEEKKAEDKLVVEEK--IAK--TQEETPMKTVK 300
            K+ E +VE+ K D    V+  +  +  EEKK EDK+ V +K  IA    ++ T  +TVK
Sbjct: 241 RKSVEAVVEDAKVDNGEEVDSGRKGKAIEEKKLEDKVAVMDKEVIASEIKEDATVTRTVK 300

Query: 301 LVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITTNEELRLAET 360
           LV  +DIRWAQLP+  +++ +R+VI+DRFP     LIKYRD EGDLVTITT +ELRLA +
Sbjct: 301 LVHGDDIRWAQLPLDSSVVLVRDVIKDRFPALKGFLIKYRDSEGDLVTITTTDELRLAAS 360

Query: 361 SNESQGSVRFYIFEVSPEKDPFYERLNTNE-VAKCKVEQSSVFENGHVLRAKEI-KMSSC 420
           + E  GS R YI EVSP ++P Y+ ++ +E   K     SSV +NG V    E  K S+ 
Sbjct: 361 TREKLGSFRLYIAEVSPNQEPTYDVIDNDESTDKFAKGSSSVADNGSVGDFVESEKASTS 420

Query: 421 IDDWIIQFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFDLAAEKFHE 480
           ++ WI QFAQLF NHVGF+S  YL+LH+LGMKLYTEA+E+ VT E+AQ LFD+AA+KF E
Sbjct: 421 LEHWIFQFAQLFKNHVGFDSDSYLELHNLGMKLYTEAMEDIVTGEDAQELFDIAADKFQE 480

Query: 481 MAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGRIYQMAIEI 540
           MAALA+F WGNV MSKAR+++YF +  S+  + ++++A ++W + EY +A   Y+ A++I
Sbjct: 481 MAALAMFNWGNVHMSKARRQIYFPEDGSRETILEKVEAGFEWAKNEYNKAAEKYEGAVKI 540

Query: 541 KPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEENMETGMKMWE 600
           K DFYE  LALGQQQFEQAKL W +A+S  VD ++    +VLKLYN AEE+ME GM++WE
Sbjct: 541 KSDFYEALLALGQQQFEQAKLCWYHALSGEVDIESDASQDVLKLYNKAEESMEKGMQIWE 600

Query: 601 EWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINLLWGTMLYERS 660
           E EE+R    S     K  LQK+GLDG+  + S  E+AEQ  NM S INLLWG++LYERS
Sbjct: 601 EMEERRLNGISNFDKHKELLQKLGLDGIFSEASDEESAEQTANMSSQINLLWGSLLYERS 660

Query: 661 ILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIDEIVQAW 720
           I+E+K+GLP W ECLEVAVEKFELAGASATDIAVM+KNHCSS+NA EG+GFKIDEIVQAW
Sbjct: 661 IVEYKLGLPTWDECLEVAVEKFELAGASATDIAVMVKNHCSSDNALEGMGFKIDEIVQAW 720

Query: 721 NEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLE 740
           NEMY+A++   GVPSFRLEPLFRRR  K++ +LE
Sbjct: 721 NEMYDAKRWQIGVPSFRLEPLFRRRSPKLHDILE 738

BLAST of Lag0034259 vs. TAIR 10
Match: AT2G25290.3 (Octicosapeptide/Phox/Bem1p (PB1) domain-containing protein / tetratricopeptide repeat (TPR)-containing protein )

HSP 1 Score: 719.2 bits (1855), Expect = 3.5e-207
Identity = 408/754 (54.11%), Postives = 522/754 (69.23%), Query Frame = 0

Query: 1   MGKQGGKKKQIGDKFREAISKHRQGGD--------GSPKSYDKDHVIFITMSQVLKEEGN 60
           MGK  GKKK               GG          + KS+D D  IFI  +  LKEEGN
Sbjct: 1   MGKPTGKKKNNNYTEMPPTESSTTGGGKTGKSFDRSATKSFDDDMTIFINRALELKEEGN 60

Query: 61  RLFQSRDLEGAILKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAVHECNLALE 120
           +LFQ RD EGA+ +Y+KA+KLLPR+H DV+YLR++MA+CYMQMGL EYP A++ECNLALE
Sbjct: 61  KLFQKRDYEGAMFRYDKAVKLLPRDHGDVAYLRTSMASCYMQMGLGEYPNAINECNLALE 120

Query: 121 VTPKYSKALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALEMKGSKD 180
            +P++SKALLKRARCYEAL++LD A RD   VLNMEP+N+ A EI ER+ + L  KG  D
Sbjct: 121 ASPRFSKALLKRARCYEALNKLDFAFRDSRVVLNMEPENVSANEIFERVKKVLVGKGI-D 180

Query: 181 VDAEIKPPLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKAGDEMVE 240
           VD E++  L  V+   A   +K  KE+ RKKK    T                 G    E
Sbjct: 181 VD-EMEKNLVNVQPVGAARLRKIVKERLRKKKKKSMTMTN--------------GGNDGE 240

Query: 241 EKADEIIVEEEKADE-IIVEEEKAEETGEEKKAEDKLVVEEK--IAK--TQEETPMKTVK 300
            K+ E +VE+ K D    V+  +  +  EEKK EDK+ V +K  IA    ++ T  +TVK
Sbjct: 241 RKSVEAVVEDAKVDNGEEVDSGRKGKAIEEKKLEDKVAVMDKEVIASEIKEDATVTRTVK 300

Query: 301 LVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVTITTNEELRLAET 360
           LV  +DIRWAQLP+  +++ +R+VI+DRFP     LIKYRD EGDLVTITT +ELRLA +
Sbjct: 301 LVHGDDIRWAQLPLDSSVVLVRDVIKDRFPALKGFLIKYRDSEGDLVTITTTDELRLAAS 360

Query: 361 SNESQGSVRFYIFEVSPEKDPFYERLNTNE-VAKCKVEQSSVFENGHVLRAKEI-KMSSC 420
           + E  GS R YI EVSP ++P Y+ ++ +E   K     SSV +NG V    E  K S+ 
Sbjct: 361 TREKLGSFRLYIAEVSPNQEPTYDVIDNDESTDKFAKGSSSVADNGSVGDFVESEKASTS 420

Query: 421 IDDWIIQFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQGLFDLAAEKFHE 480
           ++ WI QFAQLF NHVGF+S  YL+LH+LGMKLYTEA+E+ VT E+AQ LFD+AA+KF E
Sbjct: 421 LEHWIFQFAQLFKNHVGFDSDSYLELHNLGMKLYTEAMEDIVTGEDAQELFDIAADKFQE 480

Query: 481 MAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAEAGRIYQMAIEI 540
           MAALA+F WGNV MSKAR+++YF +  S+  + ++++A ++W + EY +A   Y+ A++I
Sbjct: 481 MAALAMFNWGNVHMSKARRQIYFPEDGSRETILEKVEAGFEWAKNEYNKAAEKYEGAVKI 540

Query: 541 KPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAEENMETGMKMWE 600
           K DFYE  LALGQQQFEQAKL W +A+S  VD ++    +VLKLYN AEE+ME GM++WE
Sbjct: 541 KSDFYEALLALGQQQFEQAKLCWYHALSGEVDIESDASQDVLKLYNKAEESMEKGMQIWE 600

Query: 601 EWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHINLLWGTMLYERS 660
           E EE+R    S     K  LQK+GLDG+  + S  E+AEQ  NM S INLLWG++LYERS
Sbjct: 601 EMEERRLNGISNFDKHKELLQKLGLDGIFSEASDEESAEQTANMSSQINLLWGSLLYERS 660

Query: 661 ILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGLGFKIDEIVQAW 720
           I+E+K+GLP W ECLEVAVEKFELAGASATDIAVM+KNHCSS+NA EG+GFKIDEIVQAW
Sbjct: 661 IVEYKLGLPTWDECLEVAVEKFELAGASATDIAVMVKNHCSSDNALEGMGFKIDEIVQAW 720

Query: 721 NEMYEARKLLTGVPSFRLEPLFRRRVSKIYHVLE 740
           NEMY+A++   GVPSFRLEPLFRRR  K++ +LE
Sbjct: 721 NEMYDAKRWQIGVPSFRLEPLFRRRSPKLHDILE 738

BLAST of Lag0034259 vs. TAIR 10
Match: AT4G32070.1 (Octicosapeptide/Phox/Bem1p (PB1) domain-containing protein / tetratricopeptide repeat (TPR)-containing protein )

HSP 1 Score: 690.6 bits (1781), Expect = 1.3e-198
Identity = 407/807 (50.43%), Postives = 530/807 (65.68%), Query Frame = 0

Query: 1   MGKQGGKKKQIGDKFREAISKHRQGGDGSPKSY--------DKDHVIFITMSQVLKEEGN 60
           MGK   KKK   +  ++A      GG  S K+Y        D+D  IFI+ +  LKEEGN
Sbjct: 1   MGKPTAKKKN-PETPKDASGGGGGGGGKSGKTYHRSTSRVFDEDMEIFISRALELKEEGN 60

Query: 61  RLFQSRDLEGAILKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEYPRAVHECNLALE 120
           +LFQ RD EGA+L ++KALKLLP++HIDV+YLR++MA+CYMQMGL EYP A+ ECNLALE
Sbjct: 61  KLFQKRDHEGAMLSFDKALKLLPKDHIDVAYLRTSMASCYMQMGLGEYPNAISECNLALE 120

Query: 121 VTPKYSKALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISERLTEALEMKGSKD 180
            +P+YSKAL++R+RCYEAL++LD A RD   VLNMEP N+ A EI +R+ + L  KG  D
Sbjct: 121 ASPRYSKALVRRSRCYEALNKLDYAFRDARIVLNMEPGNVSANEIFDRVKKVLVDKGI-D 180

Query: 181 VDAEIKPPLDFVELPAAVLPQKRPKEKSRKKKNNQKTKETIDEKKADEIVEEKAGDEM-- 240
           VD   K  +D   + AA L +K  KE+ RK K  +K+    +E K+ ++V    GDE   
Sbjct: 181 VDEMEKDFVDVQPVCAARL-KKIVKERLRKSKKKKKSGGKDEELKSPKVVVVDKGDEAEG 240

Query: 241 ----VEEKADEIIVE-------EE-----KADEIIVEEEKAEETGEEKKAEDKLVVEEKI 300
                EEK+D+  ++       EE     K+D+   ++    + GEE+K EDK+VV +K 
Sbjct: 241 RNKPKEEKSDKSDIDGKIGGKREEKKTSFKSDKGQKKKSGGNKAGEERKVEDKVVVMDKE 300

Query: 301 -----------AKTQEETPMKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVL 360
                      +K +  T  +T+KLV  +DIRWAQLP+  T+  +R+VIRDRFP     L
Sbjct: 301 VIASEIVDGGGSKKEGATVTRTIKLVHGDDIRWAQLPLDSTVRLVRDVIRDRFPALRGFL 360

Query: 361 IKYRDEEGDLVTITTNEELRLAETSNESQGSVRFYIFEVSPEKDPFYERL-NTNEVAKCK 420
           IKYRD EGDLVTITT +ELRLA ++++  GS+R YI EV+P+++P Y+ + NT    K  
Sbjct: 361 IKYRDTEGDLVTITTTDELRLAASTHDKLGSLRLYIAEVNPDQEPTYDGMSNTESTDKVS 420

Query: 421 VEQSSVFENGHVLR-AKEIKMSSCIDDWIIQFAQLFINHVGFESGPYLDLHDLGMKLYTE 480
              SS+ +NG V       K S C ++WI QFAQLF NHVGF+S  Y+DLHDLGMKLYTE
Sbjct: 421 KRLSSLADNGSVGEYVGSDKASGCFENWIFQFAQLFKNHVGFDSDSYVDLHDLGMKLYTE 480

Query: 481 AVEETVTSEEAQGLFDLAAEKFHEMAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQI 540
           A+E+ VT E+AQ LF +AA+KF EM ALAL  WGNV MSKARK+V   + AS+  + + +
Sbjct: 481 AMEDAVTGEDAQELFQIAADKFQEMGALALLNWGNVHMSKARKQVCIPEDASREAIIEAV 540

Query: 541 KAAYDWVEKEYAEAGRIYQMAIEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTL 600
           +AA+ W + EY +A   Y+ AI++KPDFYE  LALGQ+QFE AKL W +A+ S VD ++ 
Sbjct: 541 EAAFVWTQNEYNKAAEKYEEAIKVKPDFYEALLALGQEQFEHAKLCWYHALKSKVDLESE 600

Query: 601 PCTEVLKLYNNAEENMETGMKMWEEWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAE 660
              EVLKLYN AE++ME GM++WEE EE R    SK    K  L+K+ LD L  + S  E
Sbjct: 601 ASQEVLKLYNKAEDSMERGMQIWEEMEECRLNGISKLDKHKNMLRKLELDELFSEASEEE 660

Query: 661 AAEQAKNMRSHINLLWGTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMI 720
             EQ  NM S INLLWG++LYERSI+E+K+GLP W ECLEVAVEKFELAGASATDIAVM+
Sbjct: 661 TVEQTANMSSQINLLWGSLLYERSIVEYKLGLPTWDECLEVAVEKFELAGASATDIAVMV 720

Query: 721 KNHCSSNNAHE-----------------------------GLGFKIDEIVQAWNEMYEAR 740
           KNHCSS +A E                             G+GFKIDEIVQAWNEMY+A+
Sbjct: 721 KNHCSSESALEGNQFLARIPNSGQVTTQWFSVYNNLRTNAGMGFKIDEIVQAWNEMYDAK 780

BLAST of Lag0034259 vs. TAIR 10
Match: AT5G20360.1 (Octicosapeptide/Phox/Bem1p (PB1) domain-containing protein / tetratricopeptide repeat (TPR)-containing protein )

HSP 1 Score: 521.5 bits (1342), Expect = 1.1e-147
Identity = 301/703 (42.82%), Postives = 459/703 (65.29%), Query Frame = 0

Query: 41  ITMSQVLKEEGNRLFQSRDLEGAILKYEKALKLLPRNHIDVSYLRSNMAACYMQMGLSEY 100
           ++ +Q LKEEGN+LFQ RD +GA+ KY +A+K+LP++H++VS++R+N+A+CYMQ+   E+
Sbjct: 123 VSKAQGLKEEGNKLFQKRDYDGAMFKYGEAIKILPKDHVEVSHVRANVASCYMQLEPGEF 182

Query: 101 PRAVHECNLALEVTPKYSKALLKRARCYEALHRLDLALRDVNAVLNMEPKNIMALEISER 160
            +A+HEC+LAL VTP ++KALLKRARCYEAL++LDLALRDV  V  ++PKN MA EI E+
Sbjct: 183 AKAIHECDLALSVTPDHNKALLKRARCYEALNKLDLALRDVCMVSKLDPKNPMASEIVEK 242

Query: 161 LTEALEMKGSKDVDAEIKPPLDFVE----LPAAVLPQKRPKEKSRKKKNNQKTKETIDEK 220
           L   LE KG +  ++ I+ P D+VE     PAA+  +       + KK+NQ     ++EK
Sbjct: 243 LKRTLESKGLRINNSVIELPPDYVEPVGASPAALWAKLGKVRVKKTKKSNQ-----VEEK 302

Query: 221 KADEIVEEKAGDEMVEEKADEIIVEEEKAD-EIIVEEEKAEETGEEKKAEDKLVVEEKIA 280
              E      G+++  EK + ++ E+ K   ++ V+ +++++  +  K ++K+++EE++ 
Sbjct: 303 SEGE------GEDVEPEKKNNVLAEKGKEKIKMKVKGKQSDKRSDTSKEQEKVIIEEELL 362

Query: 281 KTQEETPMKTVKLVFEEDIRWAQLPIGCTLLQLREVIRDRFPTSTAVLIKYRDEEGDLVT 340
               E   K VK V+ +DIR A+LPI CTL +LREV+ +RFP+  AV IKYRD+EGDLVT
Sbjct: 363 VIGVEDVNKDVKFVYSDDIRLAELPINCTLFKLREVVHERFPSLRAVHIKYRDQEGDLVT 422

Query: 341 ITTNEELRLAETSNESQGSVRFYIFEVSPEKDPFYERLNTNEVAKCKVEQSSVFENGHVL 400
           ITT+EELR++E S+ SQG++RFY+ EVSPE+DPF+ RL   E+ K K+   S        
Sbjct: 423 ITTDEELRMSEVSSRSQGTMRFYVVEVSPEQDPFFGRL--VEMKKLKITADS-------F 482

Query: 401 RAKEIKMSSC-IDDWIIQFAQLFINHVGFESGPYLDLHDLGMKLYTEAVEETVTSEEAQG 460
           +AK      C ++DW+I+FA LF      +S   L+L +LGMKL +EA+EE VTS+ AQG
Sbjct: 483 KAKVNGRGGCKVEDWMIEFAHLFKIQARIDSDRCLNLQELGMKLNSEAMEEVVTSDAAQG 542

Query: 461 LFDLAAEKFHEMAALALFYWGNVIMSKARKKVYFTDGASKAPLFDQIKAAYDWVEKEYAE 520
            FD AA++F E+AA +L   G V MS ARK++    G S   + +Q+K AY+  +KE+A 
Sbjct: 543 PFDRAAQQFQEVAARSLLNLGYVHMSGARKRLSLLQGVSGESVSEQVKTAYECAKKEHAN 602

Query: 521 AGRIYQMAIEIKPDFYEGYLALGQQQFEQAKLSWLYAVSSNVDSKTLPCTEVLKLYNNAE 580
           A   Y+ A++IKP+ +E +LALG QQFE+A+LSW Y + S++D KT P  +V++ Y +AE
Sbjct: 603 AKEKYEEAMKIKPECFEVFLALGLQQFEEARLSWYYVLVSHLDLKTWPYADVVQFYQSAE 662

Query: 581 ENMETGMKMWEEWEEQRTGEFSKSSNVKTQLQKMGLDGLIKDISAAEAAEQAKNMRSHIN 640
            N++  M++ E  E  +  E S++        +  L    ++      A++A  ++S I+
Sbjct: 663 SNIKKSMEVLENLETGKESEPSQAGKTDCLTHEKDLGSSTQN----NPAKEAGRLKSWID 722

Query: 641 LLWGTMLYERSILEFKMGLPVWHECLEVAVEKFELAGASATDIAVMIKNHCSSNNAHEGL 700
           +L   +LYERSI+E+K+  P W E LE A+EKFELAG    D+  +I     + N    +
Sbjct: 723 ILLCAVLYERSIMEYKLDQPFWRESLEAAMEKFELAGTCKDDVVEIISEDYVAGNTLRDI 782

Query: 701 GFKIDEIVQAWNEMYEARKLLTGVPSFRLEPLFRRRVSKIYHV 738
            F ++EI+Q ++E+YEA+    G+PS +LE + +RR   I+HV
Sbjct: 783 RFHMEEIIQIFDEIYEAKHWTNGIPSDQLEEILKRRAENIFHV 801

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022969128.10.0e+0086.13protein PHOX1-like [Cucurbita maxima] >XP_022969137.1 protein PHOX1-like [Cucurb... [more]
KAG6587865.10.0e+0085.99Protein PHOX1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021753.1 Pro... [more]
XP_022932878.10.0e+0085.86protein PHOX1-like [Cucurbita moschata] >XP_022932887.1 protein PHOX1-like [Cucu... [more]
XP_038879044.10.0e+0086.98protein PHOX1-like isoform X1 [Benincasa hispida][more]
XP_023530345.10.0e+0085.60protein PHOX1-like isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
F4IRM44.9e-20654.11Protein PHOX1 OS=Arabidopsis thaliana OX=3702 GN=PHOX1 PE=1 SV=1[more]
F4JTI14.3e-20252.31Protein PHOX4 OS=Arabidopsis thaliana OX=3702 GN=PHOX4 PE=2 SV=1[more]
K7TQE32.2e-15844.02HSP-interacting protein OS=Zea mays OX=4577 GN=HIP PE=1 SV=1[more]
F4K4871.5e-14642.82Protein PHOX3 OS=Arabidopsis thaliana OX=3702 GN=PHOX3 PE=1 SV=1[more]
O488027.8e-11938.67Protein CLMP1 OS=Arabidopsis thaliana OX=3702 GN=CLMP1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HVI30.0e+0086.13protein PHOX1-like OS=Cucurbita maxima OX=3661 GN=LOC111468214 PE=4 SV=1[more]
A0A6J1EXN10.0e+0085.86protein PHOX1-like OS=Cucurbita moschata OX=3662 GN=LOC111439405 PE=4 SV=1[more]
A0A6J1BZI40.0e+0084.75protein PHOX1-like OS=Momordica charantia OX=3673 GN=LOC111007136 PE=4 SV=1[more]
A0A6J1JHE20.0e+0085.31protein PHOX1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485121 PE=4 S... [more]
A0A6J1JJD60.0e+0084.36protein PHOX1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485121 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT2G25290.13.5e-20754.11Octicosapeptide/Phox/Bem1p (PB1) domain-containing protein / tetratricopeptide r... [more]
AT2G25290.23.5e-20754.11Octicosapeptide/Phox/Bem1p (PB1) domain-containing protein / tetratricopeptide r... [more]
AT2G25290.33.5e-20754.11Octicosapeptide/Phox/Bem1p (PB1) domain-containing protein / tetratricopeptide r... [more]
AT4G32070.11.3e-19850.43Octicosapeptide/Phox/Bem1p (PB1) domain-containing protein / tetratricopeptide r... [more]
AT5G20360.11.1e-14742.82Octicosapeptide/Phox/Bem1p (PB1) domain-containing protein / tetratricopeptide r... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 199..219
NoneNo IPR availableGENE3D3.10.20.90coord: 283..365
e-value: 2.6E-9
score: 38.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 194..215
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..32
NoneNo IPR availablePANTHERPTHR46183:SF16PROTEIN PHOX3coord: 1..740
NoneNo IPR availableCDDcd05992PB1coord: 285..360
e-value: 3.76841E-10
score: 54.9765
NoneNo IPR availableSUPERFAMILY54277CAD & PB1 domainscoord: 280..375
IPR000270PB1 domainSMARTSM00666PB1_newcoord: 283..362
e-value: 1.8E-15
score: 67.4
IPR000270PB1 domainPFAMPF00564PB1coord: 285..361
e-value: 7.1E-15
score: 54.7
IPR000270PB1 domainPROSITEPS51745PB1coord: 283..362
score: 15.641794
IPR019734Tetratricopeptide repeatSMARTSM00028tpr_5coord: 44..77
e-value: 5.4
score: 16.0
coord: 82..117
e-value: 46.0
score: 9.1
coord: 118..151
e-value: 0.018
score: 24.2
IPR019734Tetratricopeptide repeatPROSITEPS50005TPRcoord: 44..77
score: 9.4994
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 42..171
e-value: 1.8E-28
score: 100.8
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 450..546
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 42..156
IPR044517Protein PHOX1-4PANTHERPTHR46183PROTEIN CLMP1coord: 1..740

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0034259.1Lag0034259.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding